Polymer nanocomposite films based on poly(vinyl pyrrolidone) incorporated with different amounts of copper oxide (CuO) nanoparticles were prepared by the solution casting technique. The PVP/CuO nanocomposites were analyzed by X-ray diffractometry (XRD), scanning electron microscopy, UV–Visible absorption spectroscopy and dielectric spectroscopy. The XRD analysis showed that the monoclinic structure of cupric oxide was maintained in the PVP host matrix. The key optical parameters, such as optical energy gap E_g, Urbach energy E_U, absorption coefficient and refractive index, were estimated based on the UV-Vis data. The optical characteristics of the nanocomposite films revealed that their transmittance and absorption were influenced by the addition of CuO nanoparticles in the PVP matrix. Incorporation of CuO nanoparticles into the PVP matrix led to a significant decrease in band gap energy and an increase in the refractive index. The dielectric and electrical behaviors of the PVP/CuO nanocomposites were analyzed over a frequency range between 10 Hz and 1 MHz. The effect of CuO loading on the dielectric parameters (dielectric constant and dielectric loss) of the metal oxide nanocomposites was also discussed. Full article

Some multi-gene panel tests have been implemented in clinical settings to guide targeted therapy in non-small-cell lung cancer (NSCLC) in Japan. The current performance of multi-gene panel tests under the condition that the Oncomine Dx Target Test (ODxTT) and Amoy Dx^® Pan Lung Cancer PCR panel (AmoyDx-multi) are available remains relatively unknown. We retrospectively reviewed consecutive patients with NSCLC, whose FFPE samples were considered for genetic testing. We assessed the submission rates, the success rates, and the driver oncogene detection rates of multi-gene panel tests. A total of 225 patients were histologically newly diagnosed with NSCLC or diagnosed with a recurrence of NSCLC without a previous multi-gene panel test at our institution. Among the 225 patients, the FFPE samples of 212 patients (94.2%) were submitted for multi-gene panel testing, including 191 samples (84.9%) for the ODxTT and 21 samples (9.3%) for the AmoyDx-multi. Among the 212 samples submitted to multi-gene panel tests, the success rate was 99.5% (211/212). The detection rate of driver oncogene alterations for all histologies was 52.4% (111/212), and that for adenocarcinoma was 69.7% (106/152). A favorable submission rate and success rate of multi-gene panel tests were shown, along with a favorable detection rate in recent clinical settings. Full article

Gas chromatography–ion mobility spectroscopy (GC-IMS) was used to analyze the volatile components in dried Hypsizygus marmoreus of different drying methods, including hot air drying (HAD), heat pump drying (HPD), heated freeze-drying (HFD), and unheated freeze-drying (UFD). A total of 116 signal peaks corresponding to 96 volatile compounds were identified, including 25 esters, 24 aldehydes, 23 alcohols, 13 ketones, 10 heterocyclic compounds, 8 carboxylic acids, 7 terpenes, 3 sulfur-containing compounds, 2 nitrogen-containing compounds, and 1 aromatic hydrocarbon. The total content of volatile compounds in H. marmoreus dried by the four methods, from highest to lowest, was as follows: HAD, HPD, HFD, and UFD. The main volatile compounds included carboxylic acids, alcohols, esters, and aldehydes. Comparing the peak intensities of volatile compounds in dried H. marmoreus using different drying methods, it was found that the synthesis of esters, aldehydes, and terpenes increased under hot drying methods such as HAD and HPD, while the synthesis of compounds containing sulfur and nitrogen increased under freeze-drying methods such as HFD and UFD. Nine common key characteristic flavor compounds of dried H. marmoreus were screened using relative odor activity values (ROAV > 1), including ethyl 3-methylbutanoate, acetic acid, 2-methylbutanal, propanal, methyl 2-propenyl sulfate, trimethylamine, 3-octanone, acetaldehide, and thiophene. In the odor description of volatile compounds with ROAV > 0.1, it was found that important flavor components such as trimethylamine, 3-octanone, (E)-2-octenal, and dimethyl disulfide are related to the aroma of seafood. Their ROAV order is HFD > UFD > HPD > HAD, indicating that H. marmoreus using the HFD method have the strongest seafood flavor. The research findings provide theoretical guidance for selecting drying methods and refining the processing of H. marmoreus. Full article

This study proposes an innovative meshless approach that merges the peridynamic differential operator (PDDO) with the generalized finite difference method (GFDM). Based on the PDDO theory, this method introduces a new nonlocal differential operator that aims to reduce the pre-assumption required for the PDDO method and simplify the calculation process. By discretizing through the particle approximation method, this technique proficiently preserves the PDDO’s nonlocal features, enhancing the numerical simulation’s flexibility and usability. Through the numerical simulation of classical elastic static problems, this article focuses on the evaluation of the calculation accuracy, calculation efficiency, robustness, and convergence of the method. This method is significantly stronger than the finite element method in many performance indicators. In fact, this study demonstrates the practicability and superiority of the proposed method in the field of elastic statics and provides a new approach to more complex problems. Full article

Recently, lumped Mach-Zehnder Modulators (MZMs) have received renewed attention due to their potential for low power consumption and compact size. However, the practicality of lumped MZMs with conventional lumped electrodes (C-LEs) is limited by their lower electro-optical (EO) bandwidth. The reduction in EO bandwidth results from the inherent trade-off between EO bandwidth and half-wave voltage length product (${\mathrm{V}}_{\mathsf{\pi }}\mathrm{L}$) within the C-LE architecture. This paper proposes a thin-film lithium niobate (TFLN)-based lumped MZM with capacitively-loaded lumped electrodes (CL-LEs). The purely linear EO effect of the LN eliminates the parasitic capacitance in the doped PN junction and enhances the EO bandwidth. Furthermore, the CL-LE structure can break the limitation between EO bandwidth and ${\mathrm{V}}_{\mathsf{\pi }}\mathrm{L}$ inherent in the C-LE design. Simulations show the proposed device achieves a high EO bandwidth of 32.4 GHz and a low ${\mathrm{V}}_{\mathsf{\pi }}\mathrm{L}$ of 1.15 V·cm. Due to the reduced capacitance and lower ${\mathrm{V}}_{\mathsf{\pi }}\mathrm{L}$, the power consumption of the device is as low as 0.1 pJ/bit. Simulation results indicate that the open-eye diagrams are achieved at 64 Gb/s for 1.5 mm TFLN lumped MZM, with an ER of 2.97 dB. Consequently, the proposed device architecture substantially enhances the performance of lumped MZMs, showing promise for application in short-reach optical interconnects within data centers. Full article

Although advanced production systems have been developed in the last 20 years, water scarcity is still a growing problem in agriculture. This study aims to evaluate the effect of different strategies that combine the application of seaweed and microbial biostimulants with regulated deficit irrigation (RDI) strategies on the irrigation water productivity (WP_I), fruit quality parameters and soil enzymatic activity in pepper plants (Capsicum annum sp.) under two commercial greenhouse conditions. In each trial, two treatments were applied: (i) irrigation according to Farmer criteria without biostimulant applications and (ii) a combined treatment of RDI and the same biostimulation program, composed of Bacillus paralicheniformis and Ascophillum nodosum extracts. RDI was applied in different phenological stages in each greenhouse after the establishment until the 1st harvest in trial 1 or during the ripening and harvest period in trial 2. On average, the irrigation was reduced by 600 m³ ha⁻¹ compared to the Farmer irrigation schedule. In both trials, biostimulation promoted an increase in fruit numbers, punctually in trial 1, leading to yield precocity, or generally in trial 2, obtaining a higher yield. Globally, WP_I was increased when RDI was combined with biostimulation. This combined treatment also enhanced the root water absorption and improved the soil enzymatic activity in both greenhouses, suggesting that nutrients in the soil would become more available to plants. Thus, the combined action of biostimulation under different RDI strategies has been proved to be a useful strategy to improve agricultural sustainability. Full article

Performance assessment of nuclear waste repositories requires state-of-the-art knowledge of radionuclide transport properties. Additionally, the short-term development under thermal pulses and the long-term development of the near field—due to influences such as gas generation—must be evaluated. Key thermal-hydro-mechanical-chemical processes are strongly coupled on different spatial and temporal scales. To understand these coupling mechanisms, numerous material models and numerical codes have been developed. However, the existing constitutive approaches—which have been adapted to describe small-scale laboratory experiments and validated against real-scale field observations—are often unable to capture long-term material behavior with sufficient precision. To build the confidence, a more comprehensive understanding of the system at micro- and macroscopic scales is required. Most observed macroscopic processes result from microscopic changes in the crystal structure and/or crystalline aggregates, as well as changes in material properties under the influence of various factors. To characterize these physical fields in crystals, microscopic investigations, such as visualization, or geophysical methods are introduced to verify the understanding at the microscale. Two cases are demonstrated for the presented concept using microscale information: one deals with the mechanically and thermally driven migration of fluid inclusions in rock salt, the other with dilatancy-controlled gas transport in water-saturated clay material. Full article

Wood volume is an important indicator in timber trading, and log diameter is one of the primary parameters used to calculate wood volume. Currently, the most common methods for measuring log diameters are manual measurement or visual estimation by log scalers, which are laborious, time consuming, costly, and error prone owing to the irregular placement of logs and large numbers of roots. Additionally, this approach can easily lead to misrepresentation of data for profit. This study proposes a model for automatic log diameter measurement that is based on deep learning and uses images to address the existing problems. The specific measures to improve the performance and accuracy of log-diameter detection are as follows: (1) A dual network model is constructed combining the Yolov3 algorithm and DeepLabv3+ architecture to adapt to different log-end color states that considers the complexity of log-end faces. (2) AprilTag vision library is added to estimate the camera position during image acquisition to achieve real-time adjustment of the shooting angle and reduce the effect of log-image deformation on the results. (3) The backbone network is replaced with a MobileNetv2 convolutional neural network to migrate the model to mobile devices, which reduces the number of network parameters while maintaining detection accuracy. The training results show that the mean average precision of log-diameter detection reaches 97.28% and the mean intersection over union (mIoU) of log segmentation reaches 92.22%. Comparisons with other measurement models demonstrate that the proposed model is accurate and stable in measuring log diameter under different environments and lighting conditions, with an average accuracy of 96.26%. In the forestry test, the measurement errors for the volume of an entire truckload of logs and a single log diameter are 1.20% and 0.73%, respectively, which are less than the corresponding error requirements specified in the industry standards. These results indicate that the proposed method can provide a viable and cost-effective solution for measuring log diameters and offering the potential to improve the efficiency of log measurement and promote fair trade practices in the lumber industry. Full article

TsaB/YeaZ represents a promising target for novel antibacterial agents due to its indispensable role in bacterial survival, high conservation within bacterial species, and absence of eukaryotic homologs. Previous studies have elucidated the role of the essential staphylococcal protein, TsaB/YeaZ, in binding DNA to mediate the transcription of the ilv-leu operon, responsible for encoding key enzymes involved in the biosynthesis of branched-chain amino acids—namely isoleucine, leucine, and valine (ILV). However, the regulation of ILV biosynthesis does not account for the essentiality of TsaB/YeaZ for bacterial growth. In this study, we investigated the impact of TsaB/YeaZ depletion on bacterial morphology and gene expression profiles using electron microscopy and deep transcriptomic analysis, respectively. Our results revealed significant alterations in bacterial size and surface smoothness upon TsaB/YeaZ depletion. Furthermore, we pinpointed specific genes and enriched biological pathways significantly affected by TsaB/YeaZ during the early and middle exponential phases and early stationary phases of growth. Crucially, our research uncovered a regulatory role for TsaB/YeaZ in bacterial autolysis. These discoveries offer fresh insights into the multifaceted biological functions of TsaB/YeaZ within S. aureus. Full article

An invasive spider from East Asia has established in the U.S. southeast (the “jorō spider,” Trichonephila clavata) and is rapidly expanding its range. Studies assessing the impact of this species are needed, including how expansive its diet is. An open question is whether monarch butterflies, Danaus plexippus, are a potential prey item for this spider, given that jorō spiders do not coexist with monarchs in their native range. Since monarch larvae feed on milkweed, they sequester cardiac glycosides into their adult tissues, rendering them unpalatable to many predators. At sites within northeast Georgia, we staged a series of trials (n = 61) where we tossed monarchs into jorō spider webs and, for comparison, performed similar trials with another aposematic species, gulf fritillary (Agraulis vanilla), and a palatable species, tiger swallowtail (Papilio glaucus). We recorded the outcome of the trials, which included whether the spider attacked or did not attack the prey. We also conducted a visual survey during the same fall season to look for evidence of jorō spiders consuming monarchs naturally. Our findings revealed that jorō spiders avoided eating monarchs; spiders only attacked monarchs 20% of the time, which was significantly less than the attack rates of similarly sized or larger butterflies: 86% for gulf fritillaries and 58% for tiger swallowtails. Some jorō spiders even removed monarchs from their webs. From our visual surveys of the surrounding area, we found no evidence of natural monarch consumption and, in general, butterflies made up only a fraction of the jorō spider diet. We conclude that jorō spiders appear to recognize monarch butterflies as being unpalatable, even without having a prior history with the species. This invokes questions about how these spiders can immediately recognize their unpalatability without touching the butterflies. Full article

Study design: Prospective study. Objective: To evaluate the influence of preoperatively assessed psychosomatic traits on postoperative pain, disability, and quality of life outcomes. Summary of background data: Anterior cervical discectomy and fusion (ACDF) is a widely employed surgical procedure for treating cervical spondylosis. Despite its effectiveness, various studies have reported non-success rates in terms of alleviating disability and pain. Psychological factors have become increasingly recognized as critical determinants of surgical outcomes in various medical disciplines. The full extent of their impact within the context of ACDF remains insufficiently explored. This case series aims to assess the influence of preoperative psychological profiling on long-term pain, disability and quality of life outcomes. Methods: We conducted a prospective cohort study of prospectively collected data from 76 consecutive patients who underwent ACDF with PEEK inter-fixed cages from July 2019 to November 2021. The preoperative psychological traits were assessed using the Symptom Checklist 90 (SCL-90) questionnaire. The Oswestry Disability Index (ODI), Visual Analogue Scale (VAS), Neck Disability index (NDI), EuroQol-5D (EQ-5D), and Short Form-36 (SF-36) were collected preoperatively, one month postoperatively, and at least one year after the surgical procedure. Results: The correlation analyses revealed associations between psychosomatic traits and multiple preoperative and postoperative outcome measures. The univariate analyses and linear regression analyses demonstrated the influence of the Global Severity Index (GSI) over the final follow-up scores for the ODI, VAS, NDI, EQ-5D, and SF-36. The GSI consistently exhibited a stronger correlation with the final follow-up pain, disability, and quality of life outcomes with respect to the correspondent preoperative values. Conclusion: This study highlights the importance of psychosomatic traits as predictive factors for ACDF outcomes and emphasizes their relevance in preoperative assessment for informing patients about realistic expectations. The findings underscore the need to consider psychological profiles in the preoperative workup, opening avenues for research into medications and psychological therapies. Recognizing the influence of psychosocial elements informs treatment strategies, fostering tailored surgical approaches and patient care. Full article

Nanoporous gold (np-Au) has found its use in applications ranging from catalysis to biosensing, where pore morphology plays a critical role in performance. While the morphology evolution of bulk np-Au has been widely studied, knowledge about its thin-film form is limited. This work hypothesizes that the mechanical compliance of the thin film substrate can play a critical role in the morphology evolution. Via experimental and finite-element-analysis approaches, we investigate the morphological variation in np-Au thin films deposited on compliant silicone (PDMS) substrates of a range of thicknesses anchored on rigid glass supports and compare those to the morphology of np-Au deposited on glass. More macroscopic (10 s to 100 s of microns) cracks and discrete islands form in the np-Au films on PDMS compared to on glass. Conversely, uniformly distributed microscopic (100 s of nanometers) cracks form in greater numbers in the np-Au films on glass than those on PDMS, with the cracks located within the discrete islands. The np-Au films on glass also show larger ligament and pore sizes, possibly due to higher residual stresses compared to the np-Au/PDMS films. The effective elastic modulus of the substrate layers decreases with increasing PDMS thickness, resulting in secondary np-Au morphology effects, including a reduction in macroscopic crack-to-crack distance, an increase in microscopic crack coverage, and a widening of the microscopic cracks. However, changes in the ligament/pore widths with PDMS thickness are negligible, allowing for independent optimization for cracking. We expect these results to inform the integration of functional np-Au films on compliant substrates into emerging applications, including flexible electronics. Full article

Introduction: Collared femoral stems in total hip arthroplasty (THA) offer reduced subsidence and periprosthetic fractures but raise concerns about fit accuracy and stem sizing. This study compares collared and non-collared stems to assess the stem–canal fill ratio (CFR) and fixation indicators, aiming to guide implant selection and enhance THA outcomes. Methods: This retrospective single-center study examined primary THA patients who received Corail cementless stems between August 2015 and October 2020, with a minimum of two years of radiological follow-up. The study compared preoperative bone quality assessments, including the Dorr classification, the canal flare index (CFI), the morphological cortical index (MCI), and the canal bone ratio (CBR), as well as postoperative radiographic evaluations, such as the CFR and component fixation, between patients who received a collared or a non-collared femoral stem. Results: The study analyzed 202 THAs, with 103 in the collared cohort and 99 in the non-collared cohort. Patients’ demographics showed differences in age (p = 0.02) and ASA classification (p = 0.01) but similar preoperative bone quality between groups, as suggested by the Dorr classification (p = 0.15), CFI (p = 0.12), MCI (p = 0.26), and CBR (p = 0.50). At the two-year follow-up, femoral stem CFRs (p = 0.59 and p = 0.27) were comparable between collared and non-collared cohorts. Subsidence rates were almost doubled for non-collared patients (19.2 vs. 11.7%, p = 0.17), however, not to a level of clinical significance. Conclusion: The findings of this study show that both collared and non-collared Corail stems produce comparable outcomes in terms of the CFR and radiographic indicators for stem fixation. These findings reduce concerns about stem under-sizing and micro-motion in collared stems. While this study provides insights into the collar design debate in THA, further research remains necessary. Full article

Oxygen-15 (β+, t_1/2 = 122 s) radiolabeled diatomic oxygen, in conjunction with positron emission tomography, is the gold standard to quantitatively measure the metabolic rate of oxygen consumption in the living human brain. We present herein a protocol for safe and effective delivery of [¹⁵O]O₂ over 200 m to a human subject for inhalation. A frugal quality control testing procedure was devised and validated. This protocol can act as a blueprint for other sites seeking to implement similar imaging programs. Full article

This study addresses the urgent environmental issue of climate change, focusing specifically on the role of local food, which is produced and consumed locally, in helping to reduce carbon emissions by eliminating the need for long-distance transportation. Through quantitative analysis, this study elucidates the potential benefits of environmental impact achieved through the consumption of local food, i.e., the significant reduction in carbon emissions. Specifically, the consumption of local food has been found to yield an annual decrease of 2,421,296 emissions, representing 2.5% of the Republic of Korea’s total greenhouse gas emissions from the transportation sector. This reduction further translates into an estimated economic value of USD 54.23 million (KRW 70.5 billion). These findings underscore the potential of local food consumption as a tangible strategy to overcome environmental problems. Moreover, the academic contribution of this study lies in its comprehensive analysis of the economic and empirical impacts of local food consumption on the environment. Moving forward, we propose policies such as supporting local food distribution networks, providing public education on local food, fostering local food industries, and implementing incentives to revitalize local food consumption. Full article

In the age of information technology and the additional computational search tools and software available, this systematic review aimed to identify potential therapeutic targets for obesity, evaluated in silico and subsequently validated in vivo. The systematic review was initially guided by the research question “What therapeutic targets have been used in in silico analysis for the treatment of obesity?” and structured based on the acronym PECo (P, problem; E, exposure; Co, context). The systematic review protocol was formulated and registered in PROSPERO (CRD42022353808) in accordance with the Preferred Reporting Items Checklist for Systematic Review and Meta-Analysis Protocols (PRISMA-P), and the PRISMA was followed for the systematic review. The studies were selected according to the eligibility criteria, aligned with PECo, in the following databases: PubMed, ScienceDirect, Scopus, Web of Science, BVS, and EMBASE. The search strategy yielded 1142 articles, from which, based on the evaluation criteria, 12 were included in the systematic review. Only seven these articles allowed the identification of both in silico and in vivo reassessed therapeutic targets. Among these targets, five were exclusively experimental, one was exclusively theoretical, and one of the targets presented an experimental portion and a portion obtained by modeling. The predominant methodology used was molecular docking and the most studied target was Human Pancreatic Lipase (HPL) (n = 4). The lack of methodological details resulted in more than 50% of the papers being categorized with an “unclear risk of bias” across eight out of the eleven evaluated criteria. From the current systematic review, it seems evident that integrating in silico methodologies into studies of potential drug targets for the exploration of new therapeutic agents provides an important tool, given the ongoing challenges in controlling obesity. Full article

The junction temperature of a power device in a natural air-cooled power converter fluctuates randomly due to the variation in airflow rate in ambient environments. Most of the existing thermal analysis methods do not pay attention to the uncertain influence of airflow on the heat-dissipation capacity of such converters, making it difficult to accurately evaluate the thermal safety of these devices. To address this issue, a thermal safety assessment method for power devices in natural air-cooled converters is proposed in this paper. In the proposed method, convective heat resistance samples of converter housing are extracted with an equivalent thermal network model and the historical operation temperature of the converter. Wavelet packet transform is used to analyze the time–frequency domain characteristics of the convective heat resistance, and Monte Carlo simulation is employed to simulate the random influence of the airflow rate on the device junction temperature. The thermal safety of power devices is assessed in the form of over-temperature probability, which is expressed by a two-variable growth function. An experimental platform is designed to validate the effectiveness of the proposed method. The results show that the proposed method can accurately estimate the over-temperature risk of a power device in a natural air-cooled converter under different ambient temperature and current levels, thus effectively improving the thermal reliability of converters. Full article

Data on germline mutations in soft tissue and bone sarcomas are scarce. We sought to identify the prevalence of germline mutations in adult sarcoma patients treated at a tertiary cancer center. Newly diagnosed patients were offered germline genetic testing via an 84-gene panel. The prevalence of pathogenic germline variants (PGVs) and their association with disease-, and patient- related factors are reported. A total of 87 patients were enrolled, the median age was 48 (19–78) years, and 47 (54%) were females. Gastrointestinal stromal tumors (n = 12, 13.8%), liposarcoma (n = 10, 11.5%), and Ewing sarcoma (n = 10, 11.5%) were the main subtypes. A total of 20 PGVs were detected in 18 (20.7%) patients. Variants of uncertain significance, in the absence of PGVs, were detected in 40 (45.9%) patients. Young age (p = 0.031), presence of a second primary cancer (p = 0.019), and female gender (p = 0.042) were correlated with the presence of PGVs. All identified PGVs have potential clinical actionability and cascade testing, and eight (44.44%) suggested eligibility for a targeted therapy. Almost one in five adult patients with soft tissue and bone sarcomas harbor pathogenic or likely pathogenic variants. Many of these variants are potentially actionable, and almost all have implications on cancer screening and family counselling. In this cohort from the Middle East, younger age, presence of a second primary tumor, and female gender were significantly associated with higher PGVs rates. Larger studies able to correlate treatment outcomes with genetic variants are highly needed. Full article

Proton magnetic resonance spectroscopy (¹H MRS) presents a powerful tool for revealing molecular-level metabolite information, complementary to the anatomical insight delivered by magnetic resonance imaging (MRI), thus playing a significant role in in vivo/in vitro biological studies. However, its further applications are generally confined by spectral congestion caused by numerous biological metabolites contained within the limited proton frequency range. Herein, we propose a pure-shift-based ¹H localized MRS method as a proof of concept for high-resolution studies of biological samples. Benefitting from the spectral simplification from multiplets to singlet peaks, this method addresses the challenge of spectral congestion encountered in conventional MRS experiments and facilitates metabolite analysis from crowded NMR resonances. The performance of the proposed pure-shift ¹H MRS method is demonstrated on different kinds of samples, including brain metabolite phantom and in vitro biological samples of intact pig brain tissue and grape tissue, using a 7.0 T animal MRI scanner. This proposed MRS method is readily implemented in common commercial NMR/MRI instruments because of its generally adopted pulse-sequence modules. Therefore, this study takes a meaningful step for MRS studies toward potential applications in metabolite analysis and disease diagnosis. Full article

To develop imaging biomarkers for tumors aggressiveness, our previous optical redox imaging (ORI) studies of the reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavoproteins (Fp, containing flavin adenine dinucleotide, i.e., FAD) in tumor xenografts of human melanoma associated the high optical redox ratio (ORR = Fp/(Fp + NADH)) and its heterogeneity to the high invasive/metastatic potential, without having reported quantitative results for NADH and Fp. Here, we implemented a calibration procedure to facilitate imaging the nominal concentrations of tissue NADH and Fp in the mouse xenografts of two human melanoma lines, an indolent less metastatic A375P and a more metastatic C8161. Images of the redox indices (NADH, Fp, ORR) revealed the existence of more oxidized areas (OAs) and more reduced areas (RAs) within individual tumors. ORR was found to be higher and NADH lower in C8161 compared to that of A375P xenografts, both globally for the whole tumors and locally in OAs. The ORR in the OA can differentiate xenografts with a higher statistical significance than the global averaged ORR. H&E staining of the tumors indicated that the redox differences we identified were more likely due to intrinsically different cell metabolism, rather than variations in cell density. Full article

Herein, we report a series of 1,3-diarylpyrazoles that are analogues of compound 26/HIT 8. We previously identified this molecule as a ‘hit’ during a high-throughput screening campaign for autophagy inducers. A variety of synthetic strategies were utilized to modify the 1,3-diarylpyrazole core at its 1-, 3-, and 4-position. Compounds were assessed in vitro to identify their cytotoxicity properties. Of note, several compounds in the series displayed relevant cytotoxicity, which warrants scrutiny while interpreting biological activities that have been reported for structurally related molecules. In addition, antiparasitic activities were recorded against a range of human-infective protozoa, including Trypanosoma cruzi, T. brucei rhodesiense, and Leishmania infantum. The most interesting compounds displayed low micromolar whole-cell potencies against individual or several parasitic species, while lacking cytotoxicity against human cells. Full article

In the realm of education, discussions surrounding the concept of “visibility” typically concentrate on students and marginalized populations, often overlooking highly positioned individuals, such as leading teachers. This research delves into the fundamental importance of visibility in the transformative process of teacher professional development as leaders. It investigates its character among teacher leaders and the experience of being seen and scrutinizes its central role in shaping a cohesive professional identity. Over a two-year period, data were gathered from 42 leading teachers in elementary and high schools using a phenomenological approach. The findings reveal a paradox: despite the visibility of teachers in their communities, expressions of a lack of professional visibility persist in their continuing professional development (CPD). However, visibility is crucial for research participants, serving as a catalyst for shaping their professional identity. This identity formation involves four key processes: recognizing one’s developmental journey, reflecting on professional experiences within a timeframe, fostering introspection, and reconstructing identity based on reflection outcomes. This iterative process signifies continuous evolution and analysis. Full article