Methanotrophy is a biological process that effectively reduces global methane emissions by utilizing microorganisms that can utilize methane as a source of energy under both oxic and anoxic conditions, using a variety of different electron acceptors. Methanotrophic microbes, which utilize methane as their primary source of carbon and energy, are microorganisms found in various environments, such as soil, sediments, freshwater, and marine ecosystems. These microbes play a significant role in the global carbon cycle by consuming methane, a potent greenhouse gas, and converting it into carbon dioxide, which is less harmful. However, methane is known to be the primary contributor to ozone formation and is considered a major greenhouse gas. Methane alone contributes to 30% of global warming; its emissions increased by over 32% over the last three decades and thus affect humans, animals, and vegetation adversely. There are different sources of methane emissions, like agricultural activities, wastewater management, landfills, coal mining, wetlands, and certain industrial processes. In view of the adverse effects of methane, urgent measures are required to reduce emissions. Methanotrophs have attracted attention as multifunctional bacteria with potential applications in biological methane mitigation and environmental bioremediation. Methanotrophs utilize methane as a carbon and energy source and play significant roles in biogeochemical cycles by oxidizing methane, which is coupled to the reduction of various electron acceptors. Methanotrophy, a natural process that converts methane into carbon dioxide, presents a promising solution to mitigate global methane emissions and reduce their impact on climate change. Nonetheless, additional research is necessary to enhance and expand these approaches for extensive use. In this review, we summarize the key sources of methane, mitigation strategies, microbial aspects, and the application of methanotrophs in global methane sinks with increasing anthropogenic methane emissions. Full article

Unsteady aerodynamic prediction at high angles of attack is of great importance to the design and development of advanced fighters. In this paper, a weighted feature fusion model (WFFM) that combines the state-space model and neural networks is proposed to build an unsteady aerodynamic model for the precise simulation and control of post-stall maneuvers. In the proposed model, the influences of the physical model on neural networks are considered and adjusted by introducing a standardization layer and a new weighting method. A long short-term memory (LSTM) network is used to fuse two mappings: one from flight states to aerodynamic loads, and the other from low-fidelity data to high-fidelity data. Data from wind tunnel oscillation experiments at high angles of attack using a new kind of wire-driven parallel robot and the traditional tail support are used for verifying the proposed aerodynamic model. The output of the WFFM is also compared with predictions from other models, such as the state-space model, single LSTM model, and feature fusion model not including a feature weighting layer. Results demonstrate improved accuracy of the proposed model in the interpolation and extrapolation tests. Furthermore, the WFFM is applied to the flight simulation of F-16 with different control inputs. Compared with conventional models, the WFFM shows improved accuracy and better generalization capability. Full article

This study addresses the challenge of accurately determining the arrival time of stress wave signals in SHPB test data processing. To eliminate human error, we introduce the time-window energy ratio method and evaluate six filters for noise reduction using box fractal dimensions. A mathematical model is established to optimize the stress equilibrium and impact process, which is solved using particle swarm optimization, resulting in the PSO-TWER method. We explore the impact of inertia weight and calculation methods on optimization outcomes, defining a stress equilibrium evaluation index. The results indicate that time-window length significantly affects arrival-time outputs, and the dynamic inertia weight factor enhances optimization convergence. The method accurately determines arrival times and effectively screens test data, providing a robust approach for SHPB test data processing. Full article

Pancreatic cancer is a type of gastrointestinal tumor with a growing incidence and mortality worldwide. Pancreatic ductal adenocarcinoma (PDAC) constitutes 90% of cases, and late-stage diagnosis is common, leading to a 5-year survival rate of less than 10% in high-income countries. The use of biomarkers has different proven translational applications, facilitating early diagnosis, accurate prognosis and identification of potential therapeutic targets. Several studies have shown a correlation between the tissue expression levels of various molecules, measured through immunohistochemistry (IHC), and survival rates in PDAC. Following the hallmarks of cancer, epigenetic and metabolic reprogramming, together with immune evasion and tumor-promoted inflammation, plays a critical role in cancer initiation and development. In this study, we aim to explore via IHC and Kaplan–Meier analyses the prognostic value of various epigenetic-related markers (histones 3 and 4 (H3/H4), histone acetyl transferase 1 (HAT-1), Anti-Silencing Function 1 protein (ASF1), Nuclear Autoantigenic Sperm Protein (NASP), Retinol Binding Protein 7 (RBBP7), importin 4 (IPO4) and IPO5), metabolic regulators (Phosphoglycerate mutase (PGAM)) and inflammatory mediators (allograft inflammatory factor 1 (AIF-1), interleukin 10 (IL-10), IL-12A and IL-18) in patients with PDAC. Also, through a correlation analysis, we have explored the possible interconnections in the expression levels of these molecules. Our results show that higher expression levels of these molecules are directly associated with poorer survival rates in PDAC patients, except in the case of IL-10, which shows an inverse association with mortality. HAT1 was the molecule more clearly associated with mortality, with a hazard risk of 21.74. The correlogram demonstrates an important correlation between almost all molecules studied (except in the case of IL-18), highlighting potential interactions between these molecules. Overall, our study demonstrates the relevance of including different markers from IHC techniques in order to identify unexplored molecules to develop more accurate prognosis methods and possible targeted therapies. Additionally, our correlation analysis reveals potential interactions among these markers, offering insights into PDAC’s pathogenesis and paving the way for targeted therapies tailored to individual patient profiles. Future studies should be conducted to confirm the prognostic value of these components in PDAC in a broader sample size, as well as to evaluate the possible biological networks connecting them. Full article

This paper addresses the design of beam position monitor (BPM) devices suitable for fourth-generation diffraction-limited X-ray storage rings. Detailed investigations of the electromagnetic (EM) phenomena occurring inside the component under various working conditions are carried out by considering different BPM EM models defined by their geometry and materials. Moving from a theoretical characterization of the common round geometry, rhomboidal structures are studied through a careful numerical analysis relying on advanced computer-aided tools. Several critical elements, such as wakefields, pick-up signal extraction, and trapped and propagating modes, are explored from the simulation point of view and from the experimental one, by deploying a manufactured microwave test bench, which is employed to measure the radio frequency behavior of a BPM prototype built at Elettra Sincrotrone Trieste. The aim of the proposed study is to identify a satisfactory tradeoff between achievable performance and practical realizability for BPM devices operating in last-generation light sources. Full article

This study aimed to compare different pancreatic enzyme preparations (PEPs) available in Germany regarding particle geometry and size, and to evaluate enzyme activity under physiologically relevant conditions in vitro. Pancreatic endocrine insufficiency is characterized by deficiency of pancreatic enzymes resulting in maldigestion. It is orally treated by pancreatic enzyme replacement therapy. The formulations differ in their physical properties and enzyme release behavior, potentially resulting in inconsistent dosages and poor interchangeability of products. A total of 25 products were analyzed for particle size and number of particles per capsule. Enzyme activities of lipase, amylase, and protease were measured by digestion of olive oil emulsion, starch, and casein, respectively. To analyze enzyme release, gastric environments were simulated by incubating PEPs at pH 1, 4, or 5. Duodenal conditions were simulated by subsequent incubation at pH 6. Regarding physical properties and enzyme release kinetics, considerable differences between different PEPs were found. Furthermore, compared to the label claim, excess lipase activity was observed for most products, reaching up to 148%. These in vitro results suggest poor interchangeability of PEPs, potentially explained by physical and release characteristics. Physicians and patients should be aware of the potential gap between label claims and the real-life performance of different PEPs. Full article

In this manuscript, we discuss fractional fuzzy Goursat problems with Caputo’s $gH$-differentiability. The second-order mixed derivative term in Goursat problems and two types of Caputo’s $gH$-differentiability pose challenges to dealing with Goursat problems. Therefore, in this study, we convert Goursat problems to equivalent systems fuzzy integral equations to deal properly with the mixed derivative term and two types of Caputo’s $gH$-differentiability. In this study, we utilize the concept of metric fixed point theory to discuss the existence of a unique solution of fractional fuzzy Goursat problems. For the useability of established theoretical work, we provide some numerical problems. We also discuss the solutions to numerical problems by conformable double Laplace transform. To show the validity of the solutions we provide 3D plots. We discuss, as an application, why fractional partial fuzzy differential equations are the generalization of usual partial fuzzy differential equations by providing a suitable reason. Moreover, we show the advantages of the proposed fractional transform over the usual Laplace transform. Full article

FGFR3::TACC3 fusion is a driver, potentially targetable, genetic alteration identified in approximately 4% of high-grade diffuse gliomas and rare cases with low-grade histology. Herein, we review the genetic and epigenetic features of these tumors and highlight the challenges in their classification and grading. Diffuse gliomas with FGFR3::TACC3 fusion display unique histopathological and molecular features, including an oligodendroglioma-like appearance, calcifications, and CD34 extravascular immunoreactivity. High-grade tumors exhibit molecular alterations and a DNA methylation profile typical of glioblastoma, suggesting that they may represent a subtype clinically characterized by a slightly better prognosis. Tumors with low-grade morphology are genetically and epigenetically heterogeneous. Some, exclusive to adults, have molecular alterations typical of glioblastoma, although most do not match any methylation classes, using version 12.5 of the Heidelberg classifier. Another group, which mostly affects children or adolescents, lacks the molecular features of glioblastoma and has a DNA methylation profile similar to that of low-grade glioneuronal tumors. In conclusion, diffuse gliomas with FGFR3::TACC3 fusion do not constitute a distinct nosological entity, owing to their genetic and epigenetic diversity. Further studies are warranted to clarify the biological aggressiveness of tumors with low-grade histology to refine the grading and determine the optimal treatment strategy. Full article

Colorectal cancer (CRC) accounts for about 10% of all cancer cases and 9% of cancer-related deaths globally. In the United States alone, CRC represents approximately 12.6% of all cancer cases, with a mortality rate of about 8%. CRC is now the first leading cause of cancer death in men younger than age 50 and second in women younger than age 50. This review delves into the genetic landscape of CRC, highlighting key mutations and their implications in disease progression and treatment. We provide an overview of the current and emerging therapeutic strategies tailored to individual genomic profiles. Full article

Dexmedetomidine is an a₂-agonist commonly used in veterinary practice. Occasionally, the administered dose of dexmedetomidine may result in insufficient sedation, and an additional dose or drug may be required. The sedative effects of seven different drugs administered at subsequent time points after an initial, insufficient dose of dexmedetomidine were evaluated. Seven adult cats participated in this crossover, blind, randomised study. The groups consisted of two consecutive doses of dexmedetomidine (15 + 10 μg/kg) (DD) or a dose of dexmedetomidine (15 μg/kg) followed by either NS 0.9% (DC-control group), tramadol 2 mg/kg (DT), butorphanol 0.2 mg/kg (DBT), buprenorphine 20 μg/kg (DBP), ketamine 2 mg/kg (DK), or midazolam 0.1 mg/kg (DM). Sedation was evaluated using the Grint sedation scale. In all groups, atipamezole was administered at the end of the evaluation, and recovery was assessed using the Lozano and Sams recovery scales. The DC and DM groups exhibited minimal sedative effects. The maximum sedative effect was observed in the DD and DK groups, while sedation in the DD and DK groups was significantly higher compared to the DC group. Recovery in all groups was uneventful, except in the DM group, where it was prolonged and difficult, although no statistically significant difference was detected. Therefore, insufficient sedation with dexmedetomidine can be enhanced by a subsequent dose of dexmedetomidine, ketamine, or butorphanol, whereas the addition of midazolam reduces sedation and prolongs recovery. Full article

An engineering approach was applied to modify the core layers of charge-trapping flash (CTF) memory—the blocking layer, charge-trapping layer, and tunneling layer. The doping of Ti in the charge-trapping layer and the use of Si-doped HfO₂ for the tunneling layer could optimize charge capture and leakage control. This design enhances programming and erasing speeds and increases overall device stability by creating more corner fields and using the Coulomb blockade effect. Experimental results demonstrate a larger memory window and better charge retention for the new device at the same charge-trapping layer thickness. These findings signify the advancement of the new CTF memory in balancing fast programming and long-term charge retention. The long-standing contradiction between charge capturing and retention could be partially resolved by using this engineering method. Full article

Individuals with intellectual disabilities have a shorter lifespan and significantly higher prevalence of conditions such as hypertension and cardiovascular diseases than healthy individuals. Thus, assessing the elements that contribute to their physical fitness is crucial. This cross-sectional study examined the relationship between the blood pressure and physical fitness of people with intellectual disabilities in South Korea, considering differences across sexes, age groups, physical attributes, and disability levels. It used data from 8502 individuals with intellectual disabilities aged 20–59 years who participated in a survey of a National Fitness Standard Center (NFSC) between 2018 and 2021. A series of t-tests, one-way analysis of variance, logistic regression, and the four-quartile method were used for data analyses. The results showed differences in physical fitness levels between men and women considering all aspects except for BMI (Body Mass Index), with men showing higher blood pressure levels. Lower grip strength, lower PEI (physical efficiency index) scores, and higher BMI were associated with increased blood pressure. Additionally, individuals with higher levels of disability tended to have lower levels of physical fitness, while higher physical fitness levels were associated with lower blood pressure. Therefore, low fitness levels and hypertension risk may be important health indicators for people with intellectual disabilities. Full article

Mass spectrometry has become the most prominent yet evolving technology in quantitative proteomics. Today, a number of label-free and label-based approaches are available for the relative and absolute quantification of proteins and peptides. However, the label-based methods rely solely on the employment of stable isotopes, which are expensive and often limited in availability. Here we propose a label-based quantification strategy, where the mass difference is identified by the differential alkylation of cysteines using iodoacetamide and acrylamide. The alkylation reactions were performed under identical experimental conditions; therefore, the method can be easily integrated into standard proteomic workflows. Using high-resolution mass spectrometry, the feasibility of this approach was assessed with a set of tryptic peptides of human serum albumin. Several critical questions, such as the efficiency of labeling and the effect of the differential alkylation on the peptide retention and fragmentation, were addressed. The concentration of the quality control samples calculated against the calibration curves were within the ±20% acceptance range. It was also demonstrated that heavy labeled peptides exhibit a similar extraction recovery and matrix effect to light ones. Consequently, the approach presented here may be a viable and cost-effective alternative of stable isotope labeling strategies for the quantification of cysteine-containing proteins. Full article

This research delves into the early nucleation stages of phycocyanin, a protein pivotal for its fluorescent properties and crystalline stability and holding considerable potential for biotechnological applications. The paper contrasts traditional crystallization methods with the innovative Langmuir–Blodgett nanotemplate approach, aiming to enhance molecular assembly and nucleation processes. The study employs Langmuir–Blodgett nanotemplates alongside second-order nonlinear imaging of chiral crystal (SONICC) spectroscopy. This combination is designed to orderly organize phycocyanin molecules and provide a sensitive visualization of early-stage crystal formation, capturing the intricate dynamics of protein crystallization. The experiments were conducted under controlled conditions, where surface pressure was maintained at 26 mN/m and barrier speed at 70 cm/min to optimize the monolayer formation at the air–water interface. The Langmuir–Blodgett method, compared to traditional vapor diffusion techniques, shows improvements in the uniformity and efficiency of nucleation. The sensitivity of SONICC spectroscopy significantly enhances the visualization of the nucleation process, revealing a more structured and uniform crystalline assembly in the early stages of formation. This method demonstrates a substantial improvement in nucleation dynamics, leading to a more orderly growth process and potentially larger, well-ordered crystals. Integrating Langmuir–Blodgett nanotemplates with SONICC spectroscopy offers a significant step in understanding protein crystallization processes with insights into the nucleation and growth of protein crystals and broad implications for refining crystallography methodologies of protein-based biomaterials, contributing to the advancement of structural biology and materials science. Full article

Trachinus draco and Trachinus radiatus are two bycatch species of low commercial value and no sufficient knowledge on their biological features. In the present study, the weight–length relationship, age, growth, and ten otolith morphometric variables of these species were investigated in the southwestern Aegean Sea for the first time. Positive allometric and isometric growth in the weight were defined in T. draco and T. radiatus. The weight–length relationship was described by the parameters α = 0.002415 and b = 3.35745 in T. draco and α = 0.007582 and b = 3.09452 in T. radiatus. The von Bertalanffy growth function parameters were L_∞ = 44.51 cm, k = 0.15 year⁻¹, and t₀ = −1.31 years for T. draco and L_∞ = 58.47 cm, k = 0.16 year⁻¹, and t₀ = −0.78 years for T. radiatus. Ten otolith variables (radius, length, width, area, perimeter, roundness, circularity, form factor, rectangularity, and ellipticity) showed a significant relationship with size for both species, except the ellipticity in T. radiatus. The mean values of all the otolith variables were higher in T. radiatus than in T. draco. The otolith of T. radiatus was found to become more rectangular with size as compared to the otolith of T. radiatus. The results of this work can support further research on the behavioral and ecological features of the two species. Full article

The mitochondrial protein IF1 is upregulated in many tumors and acts as a pro-oncogenic protein through its interaction with the ATP synthase and the inhibition of apoptosis. We have recently characterized the molecular nature of the IF1–Oligomycin Sensitivity Conferring Protein (OSCP) subunit interaction; however, it remains to be determined whether this interaction could be targeted for novel anti-cancer therapeutic intervention. We generated mitochondria-targeting peptides to displace IF1 from the OSCP interaction. The use of one selective peptide led to displacement of the inhibitor IF1 from ATP synthase, as shown by immunoprecipitation. NMR spectroscopy analysis, aimed at clarifying whether these peptides were able to directly bind to the OSCP protein, identified a second peptide which showed affinity for the N-terminal region of this subunit overlapping the IF1 binding region. In situ treatment with the membrane-permeable derivatives of these peptides in HeLa cells, that are silenced for the IF1 inhibitor protein, showed significant inhibition in mitochondrial permeability transition and no effects on mitochondrial respiration. These peptides mimic the effects of the IF1 inhibitor protein in cancer HeLa cells and confirm that the IF1–OSCP interaction inhibits apoptosis. A third peptide was identified which counteracts the anti-apoptotic role of IF1, showing that OSCP is a promising target for anti-cancer therapies. Full article

In this study, two “on–off” probes (BF₂-cur-Ben and BF₂-cur-But) recognizing acetylcholinesterase (AChE) were designed and synthesized. The obtained probes can achieve recognition of AChE with good selectivity and pH-independence with a linear range of 0.5~7 U/mL and 0.5~25 U/mL respectively. BF₂-cur-Ben has a lower limit of detection (LOD) (0.031 U/mL), higher enzyme affinity (K_m = 16 ± 1.6 μM), and higher inhibitor sensitivity. A responsive mechanism of the probes for AChE was proposed based on HPLC and mass spectra (MS) experiments, as well as calculations. In molecular simulation, BF₂-cur-Ben forms more hydrogen bonds (seven, while BF₂-cur-But has only four) and thus has a more stable enzyme affinity, which is mirrored by the results of the comparison of K_m values. These two probes could enable recognition of intracellular AChE and probe BF₂-cur-Ben has superior cell membrane penetration due to its higher log p value. These probes can monitor the overexpression of AChE during apoptosis of lung cancer cells. The ability of BF₂-cur-Ben to monitor AChE in vivo was confirmed by a zebrafish experiment. Full article

To meet the requirements of achieving higher efficiency and lower NOx pollution, the flame temperature in gas turbine combustors increases continually; thus, the effusion-cooling technology has been used to ensure the combustor liner remains within the allowed temperature, by which the combustion characteristics and emission behavior are possibly influenced. In order to investigate the effects of dome cooling air flow on combustion characteristics and NOx emissions, three-dimensional combustion simulations for a swirl-stabilized can-type gas turbine combustor are carried out in this work by using the computational fluid dynamics (CFD) method. Through adjusting the ratio of the dome cooling air flow and the dilution cooling air flow, the characteristics of flow field, temperature distribution and NOx emissions under each work condition are analyzed. At different ratios of the dome-cooling air flow to the total air flow, the flow velocity field in the region near the center of the combustion chamber is not changed much, while the velocity field near the chamber wall shows a more significant difference. The temperature in the outer recirculation zone within the combustion chamber is effectively reduced as the dome cooling air flow increases. By analyzing the distribution characteristics of the concentration of OH*, it is demonstrated that the dome cooling air flow does not have a direct effect on the reaction of combustion. It is also found that as the ratio of the dome cooling air flow to the total air flow increases from 0 to 0.15, the value of the NOx emissions drops from 28.4 to 26.3 ppmv, about a 7.4% decrease. The distribution of the NOx generation rate in the combustion chamber does not vary significantly with the increasing dome cooling air flow. Furthermore, by calculating the residence time in different stages, when the the ratio of the dome cooling air flow to the total air flow varies from 0 to 0.15, the residence time in the pilot stage decreases obviously, from 42 ms to 18 ms. This means that reduction in residence time is the main factor in the decrease of NOx emissions when the dome cooling air flow increases. Full article

The perpetual significance of the pharmaceutical industry in society necessitates ongoing research efforts to enhance the efficacy of its manufacturing processes. Given that drug product manufacturing typically involves powder processing, a thorough understanding of powder characterization is needed for optimal process performance. Powder rheology is commonly examined in pharmaceutical manufacturing to elucidate the relationship between powder properties and the performance of pharmaceutical processes. This paper provides a brief discussion of recent literature regarding the various powder properties and characterization techniques encompassed in powder rheology. The powder properties are categorized into particle size, particle morphology, friability, electrostatics, permeability, wettability, cohesion, bulk density, and agglomeration sections. A distinct focus is placed on the segment about powder wettability. This review informs readers about the fundamental properties of powders known to influence pharmaceutical processes. It discusses the interrelationships among these properties, powder characterization techniques, and ideal states of powder properties that lead to optimal process performance. Full article

This paper explores the life and teachings of the renowned Chinese Buddhist monk Master Hongyi (1880–1942), focusing on his transformative encounters with impermanence and their relevance for contemporary death education. Drawing upon historical records, personal writings, and the accounts of his contemporaries, this study traces Master Hongyi’s profound spiritual journey from intense grief and existential crisis to enlightened equanimity in the face of mortality. It examines how his skillful application of Buddhist practices enabled him to find meaning, purpose, and liberation amidst the challenges of aging, illness, and dying. Through an in-depth analysis of Master Hongyi’s wisdom and lived experience, this paper proposes the “Hongyi Model”, an innovative paradigm for integrating the spiritual, psychological, and artistic dimensions of his approach into modern death education. The findings underscore the transformative potential of Buddhist teachings for fostering a more authentic, meaningful, and spiritually grounded engagement with mortality, offering valuable insights for educators, counselors, and healthcare professionals working in end-of-life care. Full article

Cervical cancer is caused by a persistent and high-grade infection. It is caused by the Human Papillomavirus (HPV), which, when entering cervical cells, alters their physiology and generates serious lesions. HPV 18 is among those most involved in carcinogenesis in this region, but there are still no drug treatments that cause cure or total remission of lesions caused by HPV. It is known that L-asparaginase is an amidohydrolase, which plays a significant role in the pharmaceutical industry, particularly in the treatment of specific cancers. Due to its antitumor properties, some studies have demonstrated its cytotoxic effect against cervical cancer cells. However, the commercial version of this enzyme has side effects, such as hypersensitivity, allergic reactions, and silent inactivation due to the formation of antibodies. To mitigate these adverse effects, several alternatives have been explored, including the use of L-asparaginase from other microbiological sources, which is the case with the use of the fungus Aspergillus niger, a high producer of L-asparaginase. The study investigated the influence of the type of fermentation, precipitant, purification, characterization, and in vitro cytotoxicity of L-asparaginase. The results revealed that semisolid fermentation produced higher enzymatic activity and protein concentration of A. niger. The characterized enzyme showed excellent stability at pH 9.0, temperature of 50 °C, resistance to surfactants and metallic ions, and an increase in enzymatic activity with the organic solvent ethanol. Furthermore, it exhibited low cytotoxicity in GM and RAW cells and significant cytotoxicity in HeLa cells. These findings indicate that L-asparaginase derived from A. niger may be a promising alternative for pharmaceutical production. Its attributes, including stability, activity, and low toxicity in healthy cells, suggest that this modified enzyme could overcome challenges associated with antitumor therapy. Full article

Speleothem δ¹³C in monsoonal China differs from speleothem δ¹⁸O, which is widely used as a climatic proxy for several complex control reasons. Nevertheless, δ¹³C records have the potential to reveal the implications of hydroclimatic changes. This study reports a speleothem δ¹³C record from Didonghe (DDH) Cave in central China spanning 34 to 13 kyr BP. After we investigated the factors that influence speleothem δ¹³C, we found that the δ¹³C record showed that DDH Cave can prompt directional shifts via local hydroclimatic changes, such as in vegetation types, biomass, and rock–water interaction processes, suggesting that δ¹³C is mainly controlled by the local hydroclimate. Ensemble empirical mode decomposition (EEMD) results revealed a coupling relationship between δ¹⁸O and δ¹³C on multiple timescales, which suggested that changes in precipitation caused by large-scale monsoonal circulation are controlled by regional hydrological conditions to a great extent. However, the hydrological conditions of the cave were relatively mild and humid during the last glacial maximum (LGM), which revealed the impact of evaporation on changes in the region’s hydrological conditions. We also found that the δ¹⁸O and δ¹³C profiles decoupled when δ¹³C changed with a shift in the location of the westerly during HS1. The δ¹³C record correlates well with other paleoclimate records, suggesting that regional hydrological conditions are also modulated by the Earth’s internal and external driving factors. Full article