Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is one of the important zoonotic and opportunistic pathogens. In recent years, there has been growing evidence that supports the potential role of S. zooepidemicus in severe diseases in horses and other animals, including humans. Furthermore, the clinical isolation and drug resistance rates of S. zooepidemicus have been increasing yearly, leading to interest in its in-depth genomic analysis. In order to deepen the understanding of the S. zooepidemicus characteristics and genomic features, we investigated the genomic islands, mobile genetic elements, virulence and resistance genes, and phenotype of S. zooepidemicus strain ZHZ 211 (ST147), isolated from an equine farm in China. We obtained a 2.18 Mb, high-quality chromosome and found eight genomic islands. According to a comparative genomic investigation with other reference strains, ZHZ 211 has more virulence factors, like an iron uptake system, adherence, exoenzymes, and antiphagocytosis. More interestingly, ZHZ 211 has acquired a mobile genetic element (MGE), prophage Ph01, which was found to be in the chromosome of this strain and included two hyaluronidase (hyl) genes, important virulence factors of the strain. Moreover, two transposons and two virulence (virD4) genes were found to be located in the same genome island of ZHZ 211. In vitro phenotypic results showed that ZHZ 211 grows faster and is resistant to clarithromycin, enrofloxacin, and sulfonamides. The higher biofilm-forming capabilities of ZHZ 211 may provide a competitive advantage for survival in its niche. The results expand our understanding of the genomic, pathogenicity, and resistance characterization of Streptococcus zooepidemicus and facilitate further exploration of its molecular pathogenic mechanism. Full article

Electromobility promises to efficiently mitigate consequences of increasing traffic volume and its accompanied greenhouse gas emissions. On an individual level, electrified bikes allow emission free electrified mobility at moderate costs, and consequently their stock has increased significantly in recent years. This simultaneously increases the demand for spare parts, which are often manufacturer- or application-specific, and due to many variants, challenging to provide for the market. This article evaluates powder-based and extrusion-based metal additive manufacturing of a typical electrified bike component. The overarching objective is to establish a sustainable spare parts supply in the field of electromobility by manufacturing spare parts in a resource-efficient and decentralized manner. This approach aims to eliminate the need for physical storage space and long transport routes for the provision of spare parts, while significantly increasing the service life of e-bikes. The investigation demonstrates how these parts can be additively manufactured function equivalent and with sufficient mechanical properties, also taking economical aspects into account. Furthermore, the needed resources and related environmental consequences for metal-based additive manufacturing spare-part production are compared for both process routes. The results show that both routes are capable of producing spare-parts at comparatively the same mechanical performance, with the mechanical performance of the initial part clearly surpassed. Furthermore, it can be observed that both routes exhibit comparable resource costs, with the powder bed fusion of metals using laser beams showing significantly lower energy and gas costs by more than ten times, but higher material costs that are approximately twice as high as those of atomic diffusion additive manufacturing. Therefore, additive manufacturing offers a promising opportunity to rapidly produce parts in small quantities which are resource efficient. Full article

For radially extended Bessel modes, the helicity density distributions of magnetic fields associated with relativistic electron vortex beams are investigated for first time in the literature. The form of the distribution is defined by the electron beam’s cylindrically symmetric density flux, which varies with the winding number ℓ and the electron spin. Different helicity distributions are obtained for different signs of the winding number $\pm \ell$, confirming the chiral nature of the magnetic fields associated with the electron vortex beam. The total current helicity for the spin-down state is smaller than that of the spin-up state. The different fields and helicities associated with opposite winding numbers and/or spin values will play an important role in the investigation of the interaction of relativistic electron vortices with matter and especially chiral matter. A comparison of the calculated quantities with the corresponding ones in the case of non-relativistic spin-polarized electron beams is performed. Full article

In this study, we synthesized a transition metal sulfide (TMS) with a spinel structure, i.e., MnIn₂S₄ (MIS), using a two-step hydrothermal and sintering process. In the context of lithium-ion battery (LIB) applications, ternary TMSs are being considered as interesting options for anode materials. This consideration arises from their notable attributes, including high theoretical capacity, excellent cycle stability, and cost-effectiveness. However, dramatic volume changes result in the electrochemical performance being severely limited, so we introduced single-walled carbon nanotubes (SWCNTs) and prepared an MIS/SWCNT composite to enhance the structural stability and electronic conductivity. The synthesized MIS/SWCNT composite exhibits better cycle performance than bare MIS. Undergoing 100 cycles, MIS only yields a reversible capacity of 117 mAh/g at 0.1 A/g. However, the MIS/SWCNT composite exhibits a reversible capacity as high as 536 mAh/g after 100 cycles. Moreover, the MIS/SWCNT composite shows a better rate capability. The current density increases with cycling, and the SWCNT composite exhibits high reversible capacities of 232 and 102 mAh/g at 2 A/g and 5 A/g, respectively. Under the same conditions, pristine MIS can only deliver reversible capacities of 21 and 4 mAh/g. The results indicate that MIS/SWCNT composites are promising anode materials for LIBs. Full article

To ensure a comfortable post-retirement life and the ability to cover living expenses, it is of utmost importance for individuals to have a clear understanding of how long their pre-retirement savings will last. In this research, we employ a ruin-theory approach to model the inflows and the outflows of retirees’ portfolios. We track all transactions within the portfolios of retired clients sourced by a registered investment provider to Canada’s Financial Wellness Lab at Western University. By utilizing an advanced ruin model, we calculate the mean and the median time it takes for savings to be exhausted, the probabilities of exhaustion of funds within the retirees’ expected remaining lifetime while accounting for the observed withdrawal rates, and the deficit at ruin if a retiree has used up all of their savings. We also account for gender as well as for the risk tolerance of retired clients using a K-Means clustering algorithm. This allows us to compare the financial outcomes for female and male retirees and to enhance some findings in the literature. In the final phase of our study, we compare the results obtained by our methodology to the 4% rule which is a widely used approach for post-retirement spending. Our results show that most retirees can withdraw safely more than they currently do (around 2.5%). A withdrawal rate of about 4.5% is proved to be safe, but it might not provide sufficient income for most retirees since it yields approximately CAD 20,000 per year for male retirees in the highest risk tolerance group who withdraw about 4.5% annually. Full article

Changes in trace element concentrations are being wildly considered when it comes to neurodegenerative disorders, such as Alzheimer’s disease and Parkinson’s disease. This study aims to present the role that trace elements play in the central nervous system. Moreover, we reviewed the mechanisms involved in their neurotoxicity. Low zinc concentrations, as well as high levels of copper, manganese, and iron, activate the signalling pathways of the inflammatory, oxidative and nitrosative stress response. Neurodegeneration occurs due to the association between metals and proteins, which is then followed by aggregate formation, mitochondrial disorder, and, ultimately, cell death. In Alzheimer’s disease, low Zn levels suppress the neurotoxicity induced by β-amyloid through the selective precipitation of aggregation intermediates. High concentrations of copper, iron and manganese cause the aggregation of intracellular α-synuclein, which results in synaptic dysfunction and axonal transport disruption. Parkinson’s disease is caused by the accumulation of Fe in the midbrain dopaminergic nucleus, and the pathogenesis of multiple sclerosis derives from Zn deficiency, leading to an imbalance between T cell functions. Aluminium disturbs the homeostasis of other metals through a rise in the production of oxygen reactive forms, which then leads to cellular death. Selenium, in association with iron, plays a distinct role in the process of ferroptosis. Outlining the influence that metals have on oxidoreduction processes is crucial to recognising the pathophysiology of neurodegenerative diseases and may provide possible new methods for both their avoidance and therapy. Full article

In this paper, a single-stage step-down power factor corrector without a full-bridge rectifier is developed, which is designed to operate in discontinuous conduction mode (DCM). In terms of control, the DCM has the advantages of simple control and easy realization, no slope compensation, zero current switching, and no diode reverse current. By sampling the output voltage and using the voltage-follower control to generate the necessary control force to drive the power switch, not only can the output voltage be stabilized at the desired value, but also the input current can be, as much as possible, in the form of a sinusoidal waveform and can follow the phase of the input voltage. Moreover, the harmonic distortion meets the requirements of the IEC6100-3-2 Class D harmonics standard, and, thus, the proposed rectifier is appropriate for the computer, computer monitor, and television receiver. Eventually, by means of mathematical deductions, simulations by PSIM version 9.1, and experimental results, the feasibility and effectiveness of the proposed circuit can be verified. Full article

In the first decades of the 20th century, the Sámi movement developed a vision for how education could play a central role in the future of the Sámi people. Faced with expanding colonial school systems, teachers and intellectuals imagined what education could look like if it was to contribute to the flourishing of Sámi livelihoods. One key contributor to this project was Per Pavelsen Fokstad (1890–1973). This article outlines key elements in Fokstad’s philosophy of education and discusses his contribution to education theory in both his contemporary cultural interface and the one that we work in over 100 years later. The analyses are based on a hermeneutical reading of Fokstad’s published texts. The analyses show how Fokstad outlined a philosophy of education based in the mother tongue as a catalyst for the child’s development of a sense of self, a feeling of community, and a connection to land. This philosophy was revolutionary in his own time due to its redefinition of what was worth learning and knowing, and has grown in significance since. Full article

This study introduces a Finite Element (FE) hybrid methodology for analyzing deepwater offshore oil and gas floating production systems. In these systems, flexible risers convey the production and are connected to a balcony on one side of the platform. The proposed methodology couples, in a cost-effective manner, the hydrodynamic model of the platform with the FE model that represents the risers and the mooring lines, considering all nonlinear dynamic interactions. The results obtained and the associated computational performance are then compared with those from traditional uncoupled analyses, which may present inaccurate results for deepwater scenarios, and from fully coupled analyses that may demand high computational costs. Moreover, particular attention is dedicated to integrating global and local stress analyses to calculate the fatigue resistance of the flexible riser. The results demonstrate that the coupled global analyses adequately capture the asymmetric behavior due to all risers being connected to one of the sides of the platform, thus resulting in a more accurate distribution of fatigue damage when compared to the uncoupled methodology. Also, fatigue life is significantly affected by adequately considering the coupling effects. Full article

Academic medical institutions seek to recruit and retain a diverse workforce to foster equitable, supportive environments in which early-stage investigators, especially those who are underrepresented in medicine, can thrive. Intersectionality is a critical theoretical framework rooted in Black feminist activism and scholarship that elucidates how power and privilege are differentially structured for groups at different intersectional sociodemographic positions. As a dynamic method of analyzing multiple axes of power and inequality, intersectionality has the potential to offer a critical lens through which to view the mentor–mentee relationship. In this article, we seek to elaborate upon and extend the concept of intersectional mentoring, elucidate its essential components, and explore its application in the context of mentoring early-stage investigators in academic medicine. We propose that intersectional mentorship requires an orientation toward deep cultural humility, lifetime learning about the impact of systemic oppressions on present-day opportunities and experiences of mentees, and changing systems that perpetuate inequities by centering praxis—the application of principles of intersectionality through action to transform power dynamics in academic culture and institutions. Intersectional mentorship can help build a more equitable and representative workforce to advance intersectionally relevant and innovative approaches to achieving health equity. Full article

Cancer comprises malignant cells surrounded by the tumor microenvironment (TME), a dynamic ecosystem composed of heterogeneous cell populations that exert unique influences on tumor development. The immune community within the TME plays a substantial role in tumorigenesis and tumor evolution. The innate and adaptive immune cells “talk” to the tumor through ligand–receptor interactions and signaling molecules, forming a complex communication network to influence the cellular and molecular basis of cancer. Such intricate intratumoral immune composition and interactions foster the application of immunotherapies, which empower the immune system against cancer to elicit durable long-term responses in cancer patients. Single-cell technologies have allowed for the dissection and characterization of the TME to an unprecedented level, while recent advancements in bioinformatics tools have expanded the horizon and depth of high-dimensional single-cell data analysis. This review will unravel the intertwined networks between malignancy and immunity, explore the utilization of computational tools for a deeper understanding of tumor–immune communications, and discuss the application of these approaches to aid in diagnosis or treatment decision making in the clinical setting, as well as the current challenges faced by the researchers with their potential future improvements. Full article

Pulmonary hypertension (PH) can arise from several distinct disease processes, with a percentage presenting with combined pre- and postcapillary pulmonary hypertension (cpcPH). Patients with cpcPH are unsuitable candidates for PH-directed therapies due to elevated pulmonary capillary wedge pressures (PCWPs); however, the PCWP is dynamic and is affected by both preload and afterload. Many patients that are diagnosed with cpcPH are hypertensive at the time of right heart catheterization which has the potential to increase the PCWP and, therefore, mimic a more postcapillary-predominant phenotype. In this small pilot study, we examine the effect of nitroprusside combined with dynamic preload augmentation with a passive leg raise maneuver in hypertensive cpcPH patients at the time of right heart catheterization to identify a more precapillary-dominant PH phenotype. Patients that met the criteria of PCWP ≤ 15 mmHg with nitroprusside infusion and PCWP ≤ 18 mmHg with nitroprusside infusion and simultaneous leg raise were started on pulmonary vascular-targeted therapy. Long-term PH therapy was well tolerated, with increased six-minute walk distance, improved WHO functional class, decreased NT-proBNP, and improved REVEAL 2.0 Lite Risk Score in this precapillary-dominant PH phenotype. This small study highlights the importance of characterizing patient physiology beyond resting conditions at the time of right heart catheterization. Full article

This study aimed to discuss the influence of specimen sizes on the compressive strength parameters of wood, specifically focusing on their compression strength, elastic modulus, and Poisson’s ratio. Therefore, three different-sized specimens (20 mm × 20 mm × 30 mm, 40 mm × 40 mm × 60 mm, 60 mm × 90 mm × 90 mm) were manufactured and tested in the longitudinal, radial, and tangential directions, following the standard testing method for acquiring the compressive strength of wood. Subsequently, based on the experimental results, compressive parameters, failure mechanisms, load–displacement curves, and stress–strain relationships were systematically analyzed for the three different-sized specimens. Meanwhile, the influence of specimen size on the compressive strength parameters of wood was also evaluated through finite element numerical simulations, utilizing the obtained mechanical parameters. The results revealed a significant correlation between compressive strength and specimen size, indicating a decrease in compressive strength with an increasing specimen size. Conversely, the elastic modulus and Poisson’s ratio exhibited less sensitivity to specimen size changes. Notably, the compressive strength parameters derived from small-sized specimens (20 mm × 20 mm × 30 mm) exhibited a lack of rationality, while those obtained from medium-sized (40 mm × 40 mm × 60 mm), and large-sized specimens (60 mm × 90 mm × 90 mm) demonstrated greater reliability, providing precise results in finite element numerical simulations. Full article

Although endometriosis is a benign disease, it is associated with cancer-related gene mutations, such as KRAS or PIK3CA. Endometriosis is associated with elevated levels of inflammatory factors that cause severe pain. In a previous study, we demonstrated that KRAS or PIK3CA mutations are associated with the activation of cell proliferation, migration, and invasion in a patient-derived immortalized endometriotic cell line, HMOsisEC10. In this study, we investigated the effects of these mutations on progesterone resistance. Since the HMOsisEC10 had suppressed progesterone receptor (PR) expression, we transduced PR-B to HMOsisEc10 cell lines including KRAS mutant and PIK3CA mutant cell lines. We conducted a migration assay, invasion assay, and MTT assay using dienogest and medroxyprogestrone acetate. All cell lines showed progesterone sensitivity with or without mutations. Regarding inflammatory factors, real-time quantitative RT-PCR revealed that the KRAS mutation cell line exhibited no suppression of Cox-2 and mPGES-1 on progesterone treatment, whereas IL-6, MCP-1, VEGF, and CYP19A1 were significantly suppressed by progesterone in both mutated cell lines. Our results suggest that KRAS mutation and PIK3CA mutation in endometriotic cells may not be associated with progesterone resistance in terms of aggressiveness. However, KRAS mutations may be associated with progesterone resistance in the context of pain. Full article

Polycaprolactone (PCL) scaffolds have demonstrated an effectiveness in articular cartilage regeneration due to their biomechanical properties. On the other hand, alginate hydrogels generate a 3D environment with great chondrogenic potential. Our aim is to generate a mixed PCL/alginate scaffold that combines the chondrogenic properties of the two biomaterials. Porous PCL scaffolds were manufactured using a modified salt-leaching method and embedded in a culture medium or alginate in the presence or absence of chondrocytes. The chondrogenic capacity was studied in vitro. Type II collagen and aggrecan were measured by immunofluorescence, cell morphology by F-actin fluorescence staining and gene expression of COL1A1, COL2A1, ACAN, COL10A1, VEGF, RUNX1 and SOX6 by reverse transcription polymerase chain reaction (RT-PCR). The biocompatibility of the scaffolds was determined in vivo using athymic nude mice and assessed by histopathological and morphometric analysis. Alginate improved the chondrogenic potential of PCL in vitro by increasing the expression of type II collagen and aggrecan, as well as other markers related to chondrogenesis. All scaffolds showed good biocompatibility in the in vivo model. The presence of cells in the scaffolds induced an increase in vascularization of the PCL/alginate scaffolds. The results presented here reinforce the benefits of the combined use of PCL and alginate for the regeneration of articular cartilage. Full article

Genetic biomarkers have played a pivotal role in the classification, prognostication, and guidance of clinical cancer therapies. Large-scale and multi-dimensional analyses of entire cancer genomes, as exemplified by projects like The Cancer Genome Atlas (TCGA), have yielded an extensive repository of data that holds the potential to unveil the underlying biology of these malignancies. Mutations stand out as the principal catalysts of cellular transformation. Nonetheless, other global genomic processes, such as alterations in gene expression and chromosomal re-arrangements, also play crucial roles in conferring cellular immortality. The incorporation of multi-omics data specific to cancer has demonstrated the capacity to enhance our comprehension of the molecular mechanisms underpinning carcinogenesis. This report elucidates how the integration of comprehensive data on methylation, gene expression, and copy number variations can effectively facilitate the unsupervised clustering of cancer samples. We have identified regressors that can effectively classify tumor and normal samples with an optimal integration of RNA sequencing, DNA methylation, and copy number variation while also achieving significant $p$-values. Further, these regressors were trained using linear and logistic regression with $k$-means clustering. For comparison, we employed autoencoder- and stacking-based omics integration and computed silhouette scores to evaluate the clusters. The proof of concept is illustrated using liver cancer data. Our analysis serves to underscore the feasibility of unsupervised cancer classification by considering genetic markers beyond mutations, thereby emphasizing the clinical relevance of additional global cellular parameters that contribute to the transformative process in cells. This work is clinically relevant because changes in gene expression and genomic re-arrangements have been shown to be signatures of cellular transformation across cancers, as well as in liver cancers. Full article

Background: Surgeons have limited knowledge of the lumbar intervertebral foramina. This study aimed to classify osteophytes in the lumbar intervertebral foramen and to determine their pathoanatomical characteristics, discuss their potential biomechanical effects, and contribute to developing surgical methods. Methods: We conducted a retrospective, non-randomized, single-center study involving 1224 patients. The gender, age, and anatomical location of the osteophytes in the lumbar intervertebral foramina of the patients were recorded. Results: Two hundred and forty-nine (20.34%) patients had one or more osteophytes in their lumbar 4 and 5 foramina. Of the 4896 foramina, 337 (6.88%) contained different types of osteophytes. Moreover, four anatomical types of osteophytes were found: mixed osteophytes in 181 (3.69%) foramina, osteophytes from the lower endplate of the superior vertebrae in 91 (1.85%) foramina, osteophytes from the junction of the pedicle and lamina of the upper vertebrae in 39 foramina (0.79%), and osteophytes from the upper endplate of the lower vertebrae in 26 (0.53%) foramina. The L4 foramen contained a significantly higher number of osteophytes than the L5 foramen. Osteophyte development increased significantly with age, with no difference between males and females. Conclusions: The findings show that osteophytic extrusions, which alter the natural anatomical structure of the lumbar intervertebral foramina, are common and can narrow the foramen. Full article

A recent measure was developed to assess the Quality of Life (QoL) of young people with advanced cancer and is available for parents and professionals (Advance QoL). The present study aimed to elaborate self-reported versions for children and adolescents with advanced cancer. We adopted a four-phase research plan: (1) to elaborate the Advance QoL questionnaire for youth (8–12 and 13–18 years old) with a team of young research partners; (2) to evaluate the understandability of these versions in a sample of 12 young patients from the target population using cognitive interviews; (3) to assess social validity in the same group using a questionnaire and the content validity index (CVI); and (4) to refine the questionnaires according to these results. Four major themes were identified: (1) issues affecting the understanding of the tool; (2) issues that did not affect the understanding of the tool; (3) modifications to improve the tool; and (4) positive features of the tool. Advance QoL was well received, and feedback was positive. Adjustments were made according to young people’s comments and two self-reported versions are now available. It is essential to measure the key domains of QoL in advanced cancer. Advance QoL self-report versions will help target the specific needs of young people with this condition and their families. Full article

Although current policies discourage the use of corporal punishment (CP), its use is still widespread in the US. The objective of this study was to assess the proportion of parents who used CP during the pandemic and identify related risk and protective factors. We analyzed results of a nationwide cross-sectional internet panel survey of 9000 US caregivers who responded in three waves from November 2020 to July 2021. One in six respondents reported having spanked their child in the past week. Spanking was associated with intimate partner violence and the use of multiple discipline strategies and not significantly associated with region or racial self-identification. Parents who spanked sought out more kinds of support, suggesting an opportunity to reduce spanking through more effective parenting resources. Additionally, these results suggest that parents who report using CP may be at risk for concurrent domestic violence. Full article

Television plays a significant role in shaping the beliefs, perceptions, and attitudes of young individuals. Understanding the factors that influence these beliefs is essential for comprehending the impact of television programming on the youth population. This paper aims to investigate the correlations in beliefs regarding television programming among Greek youths aged 15–18 with their demographic characteristics, parental educational levels, school performance, and daily television viewing habits. To study the above, a structured questionnaire was electronically distributed to 204 Greek teenagers after obtaining parental consent. By identifying the factors associated with variations in beliefs, this study contributes to the existing literature on media influence and youth development. The findings indicate youth’s moderate levels of reflection regarding different aspects of television shows, including their presentation to the public and their influence on perceptions and attitudes. What is more, the youth audience haslow trust in TV news, and they moderately believe that the content of news is greatly influenced by governments and political elite agendas, while they consider TV as a major influential media type in shaping people’s political opinions. Notably, youngsters who considered themselves high-achieving students, as well as those who engaged in more frequent television viewing, including documentaries, news, and talk shows, exhibit increased concern and skepticism regarding the information presented in the television programs they watch. This is an important finding, because despite the low trust in TV as a medium, it continues to attract the attention of young people. Full article