The 2023 MDPI Annual Report has
been released!
 
20 pages, 472 KiB  
Article
Are Consumers Equally Willing to Pay More for Brands That Aim for Sustainability, Positive Societal Contribution, and Inclusivity as for Brands That Are Perceived as Exclusive? Generational, Gender, and Country Differences
by Frank Goedertier, Bert Weijters and Joeri Van den Bergh
Sustainability 2024, 16(9), 3879; https://doi.org/10.3390/su16093879 (registering DOI) - 06 May 2024
Abstract
This study explores consumer preferences for brands that emphasize sustainability and inclusivity, and for brands perceived as exclusive and trendy. Consumer data obtained via a large-scale survey involving 24,798 participants across 20 countries and one special administrative region (SAR) are used to understand [...] Read more.
This study explores consumer preferences for brands that emphasize sustainability and inclusivity, and for brands perceived as exclusive and trendy. Consumer data obtained via a large-scale survey involving 24,798 participants across 20 countries and one special administrative region (SAR) are used to understand how willingness to pay (WTP) for these brand types varies globally, accounting for demographic factors like generation, gender, and country. A substantial body of literature highlights growing consumer interest in brands that stand for sustainability and inclusivity, challenging traditional notions that luxury and exclusivity primarily drive brand value. Despite persistent skepticism among some business executives about consumers’ actual versus claimed willingness to spend more for sustainable and inclusive brands, academics and commercial researchers increasingly signal a shift in purchasing behavior that is influenced by socio-ecological factors. This research aims to provide empirical data on consumer WTP across different demographics and countries/regions, thereby contributing to academic discussions and offering insights for managerial decision making. The study frames its investigation around four research questions, to explore how consumers’ WTP for exclusive and inclusive brands varies across generations, genders, and countries/regions. It employs a robust methodological approach, using confirmatory factor analysis (CFA) and structural equation modeling (SEM) to analyze the data. This ensures that the constructs of brand inclusiveness and exclusivity are comparable across diverse cultural contexts. Significant gender, generational, and country/region differences are observed. When comparing generations, the findings indicate that GenZ consumers have a higher WTP for sustainable/inclusive brands (compared to older, GenX, and Baby Boomer generations). Similar patterns are found when considering WTP for exclusive, on-trend brands. In terms of gender, women are observed to have a higher WTP for sustainable/inclusive brands, but a lower WTP for exclusive, on-trend brands compared to men. Finally, compared to consumers originating from certain European countries, we find that consumers living in certain Asian countries/regions have a significantly higher WTP for inclusive and sustainable brands, as well as for exclusive/on-trend brands. The study underscores the complexities of consumer behavior in the global market, highlighting the coexistence of traditional preferences for exclusive, trendy brands and preferences for brands that embrace sustainability and inclusivity. Full article
29 pages, 10645 KiB  
Article
Seeds Priming with Melatonin Improves Root Hydraulic Conductivity of Wheat Varieties under Drought, Salinity, and Combined Stress
by Yuanyuan Fu, Penghui Li, Zhuanyun Si, Shoutian Ma and Yang Gao
Int. J. Mol. Sci. 2024, 25(9), 5055; https://doi.org/10.3390/ijms25095055 (registering DOI) - 06 May 2024
Abstract
Drought and salinity stress reduce root hydraulic conductivity of plant seedlings, and melatonin application positively mitigates stress-induced damage. However, the underlying effect of melatonin priming on root hydraulic conductivity of seedlings under drought–salinity combined remains greatly unclear. In the current report, we investigated [...] Read more.
Drought and salinity stress reduce root hydraulic conductivity of plant seedlings, and melatonin application positively mitigates stress-induced damage. However, the underlying effect of melatonin priming on root hydraulic conductivity of seedlings under drought–salinity combined remains greatly unclear. In the current report, we investigated the influence of seeds of three wheat lines’ 12 h priming with 100 μM of melatonin on root hydraulic conductivity (Lpr) and relevant physiological indicators of seedlings under PEG, NaCl, and PEG + NaCl combined stress. A previous study found that the combined PEG and NaCl stress remarkably reduced the Lpr of three wheat varieties, and its value could not be detected. Melatonin priming mitigated the adverse effects of combined PEG + NaCl stress on Lpr of H4399, Y1212, and X19 to 0.0071 mL·h−1·MPa−1, 0.2477 mL·h−1·MPa−1, and 0.4444 mL·h−1·MPa−1, respectively, by modulating translation levels of aquaporin genes and contributed root elongation and seedlings growth. The root length of H4399, Y1212, and X19 was increased by 129.07%, 141.64%, and 497.58%, respectively, after seeds pre-treatment with melatonin under PEG + NaCl combined stress. Melatonin -priming appreciably regulated antioxidant enzyme activities, reduced accumulation of osmotic regulators, decreased levels of malondialdehyde (MDA), and increased K+ content in stems and root of H4399, Y1212, and X19 under PEG + NaCl stress. The path investigation displayed that seeds primed with melatonin altered the modification of the path relationship between Lpr and leaf area under stress. The present study suggested that melatonin priming was a strategy as regards the enhancement of root hydraulic conductivity under PEG, NaCl, and PEG + NaCl stress, which efficiently enhanced wheat resistant to drought–salinity stress. Full article
(This article belongs to the Special Issue Plant Adaptation Mechanism to Stress)
19 pages, 2883 KiB  
Article
Genetic Variability and Genetic Differentiation of Populations in the Grooved Carpet Shell Clam (Ruditapes decussatus) Based on Intron Polymorphisms
by Carlos Saavedra and David Cordero
Oceans 2024, 5(2), 257-275; https://doi.org/10.3390/oceans5020016 (registering DOI) - 06 May 2024
Abstract
The grooved carpet-shell clam is one of the most economically relevant shellfish species living in the Mediterranean and nearby Atlantic coasts. Previous studies using different types of genetic markers showed a remarkable genetic divergence of the eastern Mediterranean, western Mediterranean, and Atlantic populations, [...] Read more.
The grooved carpet-shell clam is one of the most economically relevant shellfish species living in the Mediterranean and nearby Atlantic coasts. Previous studies using different types of genetic markers showed a remarkable genetic divergence of the eastern Mediterranean, western Mediterranean, and Atlantic populations, but important details remained unclear. Here, data from six nuclear introns scored for restriction fragment size polymorphisms in eight populations that have not been studied before have been pooled for the analysis with data scattered through three previous studies, totaling 32 samples from 29 locations. The results show lower levels of heterozygosity, higher mean number of alleles, and alleles with restricted distribution in the Mediterranean populations, suggesting the existence of a large, isolated population in the eastern Mediterranean at the middle Pleistocene. The data also confirm the similarity of populations from Tunisia to Western Mediterranean populations. Finally, a genetic mosaic is apparent in the Atlantic coasts of the Iberian Peninsula, with a divergence of Rias Baixas populations from more northern populations and Central Portugal populations. The effects of oceanic fronts, seasonal upwellings, river plumes, and/or fishery management operations could explain this and other features of the Atlantic populations. Full article
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9 pages, 374 KiB  
Article
Effect of Fluid Intake on Acute Changes in Plasma Volume: A Randomized Controlled Crossover Pilot Trial
by Janis Schierbauer, Sabrina Sanfilippo, Auguste Grothoff, Ulrich Fehr, Nadine Wachsmuth, Thomas Voit, Paul Zimmermann and Othmar Moser
Metabolites 2024, 14(5), 263; https://doi.org/10.3390/metabo14050263 (registering DOI) - 06 May 2024
Abstract
Plasma volume (PV) undergoes constant and dynamic changes, leading to a large intra-day variability in healthy individuals. Hydration is known to induce PV changes; however, the response to the intake of osmotically different fluids is still not fully understood. In a randomized controlled [...] Read more.
Plasma volume (PV) undergoes constant and dynamic changes, leading to a large intra-day variability in healthy individuals. Hydration is known to induce PV changes; however, the response to the intake of osmotically different fluids is still not fully understood. In a randomized controlled crossover trial, 18 healthy individuals (10 females) orally received an individual amount of an isotonic sodium-chloride (ISO), Ringer (RIN), or glucose (GLU) solution. Hemoglobin mass (Hbmass) was determined with the optimized carbon monoxide re-breathing method. Fluid-induced changes in PV were subsequently calculated based on capillary hemoglobin concentration ([Hb]) and hematocrit (Hct) before and then every 10 minutes until 120 min (t0–120) after the fluid intake and compared to a control trial arm (CON), where no fluid was administered. Within GLU and CON trial arms, no statistically significant differences from baseline until t120 were found (p > 0.05). In the ISO trial arm, PV was significantly increased at t70 (+138 mL, p = 0.01), t80 (+191 mL, p < 0.01), and t110 (+182 mL, p = 0.01) when compared to t0. Moreover, PV in the ISO trial arm was significantly higher at t70 (p = 0.02), t110 (p = 0.04), and t120 (p = 0.01) when compared to the same time points in the CON trial arm. Within the RIN trial arm, PV was significantly higher between t70 and t90 (+183 mL, p = 0.01) and between t110 (+194 mL, p = 0.03) and t120 (+186 mL, p < 0.01) when compared to t0. These results demonstrated that fluids with a higher content of osmotically active particles lead to acute hemodilution, which is associated with a decrease in [Hb] and Hct. These findings underpin the importance of the hydration state on PV and especially on PV constituent levels in healthy individuals. Full article
28 pages, 4708 KiB  
Article
Neural Network Approximation of Helicopter Turboshaft Engine Parameters for Improved Efficiency
by Serhii Vladov, Ruslan Yakovliev, Maryna Bulakh and Victoria Vysotska
Energies 2024, 17(9), 2233; https://doi.org/10.3390/en17092233 (registering DOI) - 06 May 2024
Abstract
The work is devoted to the development of a method for neural network approximation of helicopter turboshaft engine parameters, which is the basis for researching engine energy characteristics to improve efficiency, reliability, and flight safety. It is proposed to use a three-layer direct [...] Read more.
The work is devoted to the development of a method for neural network approximation of helicopter turboshaft engine parameters, which is the basis for researching engine energy characteristics to improve efficiency, reliability, and flight safety. It is proposed to use a three-layer direct propagation neural network with linear neurons in the output layer for training in which the scale conjugate gradient algorithm is modified by introducing a moment coefficient into the analytical expression. This modification helps in calculating new model parameters to avoid falling into a local minimum. The dependence of the energy released during helicopter turboshaft engine compressor rotation on the gas-generator rotor r.p.m. was obtained. This enables the determination of the optimal gas-generator rotor r.p.m. region for a specific type of helicopter turboshaft engine. The optimal ratio of energy consumption and compressor operating efficiency is achieved, thereby ensuring helicopter turboshaft engines’ optimal performance and reliability. Experimental data support the high efficiency of using a three-layer feed-forward neural network with linear neurons in the output layer, trained using a modified scale conjugate gradient algorithm, for approximating parameters of helicopter turboshaft engines compared to the analogues. Specifically, this method better predicts the relations between the energy release during compressor rotation and gas-generator rotor r.p.m. The efficiency coefficient of the proposed method was 0.994, which exceeded that of the closest analogue (0.914) by 1.09 times. Full article
(This article belongs to the Section I2: Energy and Combustion Science)
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11 pages, 7896 KiB  
Case Report
Lichen Amyloidosis in an Atopic Patient Treated with Dupilumab: A New Therapeutic Option
by Benedetta Tirone, Gerardo Cazzato, Francesca Ambrogio, Caterina Foti and Marco Bellino
Diseases 2024, 12(5), 94; https://doi.org/10.3390/diseases12050094 (registering DOI) - 06 May 2024
Abstract
Lichen amyloidosis (LA) is a type of cutaneous amyloidosis characterized by brownish hyperkeratotic and itchy papules on the lower leg, back, forearm, or thigh. It is associated with itching and atopic dermatitis (AD) according to an etiopathogenetic mechanism that has not yet been [...] Read more.
Lichen amyloidosis (LA) is a type of cutaneous amyloidosis characterized by brownish hyperkeratotic and itchy papules on the lower leg, back, forearm, or thigh. It is associated with itching and atopic dermatitis (AD) according to an etiopathogenetic mechanism that has not yet been fully elucidated. Currently, the available therapies for this condition include oral antihistamines, laser, cyclosporine, topical corticosteroids, and phototherapy, but, in light of the overlap with AD, Dupilumab may also be indicated. We report the case of a female, 52 years old, who had been suffering from AD and LA for about 27 years. She had lesions attributable to both diseases on the trunk and lower limbs associated with severe itching and had proved resistant to cyclosporine therapy. It was decided to opt for Dupilumab with the induction of 2 fl of 300 mg and maintenance with 1 fl every other week. The therapy proved to be effective, returning a total resolution of both diseases one year after the beginning of the treatment. Dupilumab demonstrated efficacy and safety in the LA related to AD and led to clinical and quality of life improvements in this patient. Therefore, Dupilumab should be considered when treating LA. Further studies should be conducted focusing on the efficacy of the drug on LA (whether or not related to AD), changes in the skin lesions after discontinuation, and the safety of long-term application. Full article
17 pages, 379 KiB  
Article
Can Crisis Periods Affect the ESG Reporting Scope? The Portuguese Euronext Entities Case
by Catarina Cepeda
J. Risk Financial Manag. 2024, 17(5), 191; https://doi.org/10.3390/jrfm17050191 (registering DOI) - 06 May 2024
Abstract
Portuguese companies are increasingly responding to the demand of stakeholders for transparent information about companies’ environmental, social, and governance (ESG) performance by issuing non-financial reports (NFRs). While the number of NFRs published annually has been increasing over the last two decades, their quality [...] Read more.
Portuguese companies are increasingly responding to the demand of stakeholders for transparent information about companies’ environmental, social, and governance (ESG) performance by issuing non-financial reports (NFRs). While the number of NFRs published annually has been increasing over the last two decades, their quality and companies’ ESG performance have been questioned, especially in times of crisis. To address these concerns, several jurisdictions have introduced mandatory NFR rules, such as the European Directive 2014/95/EU. Employing an institutional theory lens, this paper’s research objective is to evaluate whether the last decade’s crises and whether the fact that NFRs became mandatory for certain entities positively affected companies’ activities covered in the ESG reporting scope. We used panel data regression models on 45 listed companies in Portugal during the period 2008–2021. Our results show that the ESG reporting scope is not positively influenced by the transition from NFRs to a mandatory and global financial crisis (GFC). However, the COVID-19 crisis positively affected NFR quality. These results have major implications for practitioners, reflecting the importance of promoting these tools in an organization to improve non-financial performance and companies’ sustainability. Full article
(This article belongs to the Special Issue Financial Reporting and Auditing)
20 pages, 663 KiB  
Review
BCM-7: Opioid-like Peptide with Potential Role in Disease Mechanisms
by Ecem Bolat, Furkan Eker, Selin Yılmaz, Sercan Karav, Emel Oz, Charles Brennan, Charalampos Proestos, Maomao Zeng and Fatih Oz
Molecules 2024, 29(9), 2161; https://doi.org/10.3390/molecules29092161 (registering DOI) - 06 May 2024
Abstract
Bovine milk is an essential supplement due to its rich energy- and nutrient-rich qualities. Caseins constitute the vast majority of the proteins in milk. Among these, β-casein comprises around 37% of all caseins, and it is an important type of casein with several [...] Read more.
Bovine milk is an essential supplement due to its rich energy- and nutrient-rich qualities. Caseins constitute the vast majority of the proteins in milk. Among these, β-casein comprises around 37% of all caseins, and it is an important type of casein with several different variants. The A1 and A2 variants of β-casein are the most researched genotypes due to the changes in their composition. It is accepted that the A2 variant is ancestral, while a point mutation in the 67th amino acid created the A1 variant. The digestion derived of both A1 and A2 milk is BCM-7. Digestion of A2 milk in the human intestine also forms BCM-9 peptide molecule. The opioid-like characteristics of BCM-7 are highlighted for their potential triggering effect on several diseases. Most research has been focused on gastrointestinal-related diseases; however other metabolic and nervous system-based diseases are also potentially triggered. By manipulating the mechanisms of these diseases, BCM-7 can induce certain situations, such as conformational changes, reduction in protein activity, and the creation of undesired activity in the biological system. Furthermore, the genotype of casein can also play a role in bone health, such as altering fracture rates, and calcium contents can change the characteristics of dietary products. The context between opioid molecules and BCM-7 points to a potential triggering mechanism for the central nervous system and other metabolic diseases discussed. Full article
(This article belongs to the Section Food Chemistry)
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15 pages, 1967 KiB  
Article
Assessing the Preservation of Lumbar Lordotic Curvature in Everyday Sitting Conditions Assessed with an Inertial Measurement System
by Ju Chan Kim, Jeong-Gil Kim, Beom Suk Kim, Cheol Ki Kim, Minseok Choi, Joonnyong Lee and Sun Gun Chung
J. Clin. Med. 2024, 13(9), 2728; https://doi.org/10.3390/jcm13092728 (registering DOI) - 06 May 2024
Abstract
Lumbar lordotic curvature (LLC), closely associated with low back pain (LBP) when decreased, is infrequently assessed in clinical settings due to the spatiotemporal limitations of radiographic methods. To overcome these constraints, this study used an inertial measurement system to compare the magnitude and [...] Read more.
Lumbar lordotic curvature (LLC), closely associated with low back pain (LBP) when decreased, is infrequently assessed in clinical settings due to the spatiotemporal limitations of radiographic methods. To overcome these constraints, this study used an inertial measurement system to compare the magnitude and maintenance of LLC across various sitting conditions, categorized into three aspects: verbal instructions, chair type, and desk task types. Methods: Twenty-nine healthy participants were instructed to sit for 3 min with two wireless sensors placed on the 12th thoracic vertebra and the 2nd sacral vertebra. The lumbar lordotic angle (LLA) was measured using relative angles for the mediolateral axis and comparisons were made within each sitting category. Results: The maintenance of LLA (LLAdev) was significantly smaller when participants were instructed to sit upright (−3.7 ± 3.9°) compared to that of their habitual sitting posture (−1.2 ± 2.4°) (p = 0.001), while the magnitude of LLA (LLAavg) was significantly larger with an upright sitting posture (p = 0.001). LLAdev was significantly larger when using an office chair (−0.4 ± 1.1°) than when using a stool (−3.2 ± 7.1°) (p = 0.033), and LLAavg was also significantly larger with the office chair (p < 0.001). Among the desk tasks, LLAavg was largest during keyboard tasks (p < 0.001), followed by mouse and writing tasks; LLAdev showed a similar trend without statistical significance (keyboard, −1.2 ± 3.0°; mouse, −1.8 ± 2.2°; writing, −2.9 ± 3.1°) (p = 0.067). Conclusions: Our findings suggest that strategies including the use of an office chair and preference for computer work may help preserve LLC, whereas in the case of cueing, repetition may be necessary. Full article
(This article belongs to the Section Clinical Rehabilitation)
27 pages, 21112 KiB  
Article
CHANG-ES XXXI—A Decade of CHANG-ES: What We Have Learned from Radio Observations of Edge-on Galaxies
by Judith Irwin, Rainer Beck, Tanden Cook, Ralf-Jürgen Dettmar, Jayanne English, Volker Heesen, Richard Henriksen, Yan Jiang, Jiang-Tao Li, Li-Yuan Lu, Crystal Mele, Ancla Müller, Eric Murphy, Troy Porter, Richard Rand, Nathan Skeggs, Michael Stein, Yelena Stein, Jeroen Stil, Andrew Strong, Rene Walterbos, Q. Daniel Wang, Theresa Wiegert and Yang Yangadd Show full author list remove Hide full author list
Galaxies 2024, 12(3), 22; https://doi.org/10.3390/galaxies12030022 (registering DOI) - 06 May 2024
Abstract
CHANG-ES (Continuum Halos in Nearby Galaxies—an EVLA Survey) is an ambitious project to target 35 nearby disk galaxies that are edge-on to the line of sight. The orientation permits both the disk and halo regions to be studied. The observations were initially at [...] Read more.
CHANG-ES (Continuum Halos in Nearby Galaxies—an EVLA Survey) is an ambitious project to target 35 nearby disk galaxies that are edge-on to the line of sight. The orientation permits both the disk and halo regions to be studied. The observations were initially at 1.5 GHz (L-band) and 6.0 GHz (C-band) in a variety of VLA array configurations, and in all four Stokes parameters, which allowed for spatially resolved images in total intensity plus polarization. The inclusion of polarization is unique to an edge-on galaxy survey and reveals the galaxies’ halo magnetic fields. This paper will summarize the results to date, some of which are new phenomena, never seen prior to CHANG-ES. For example, we see that ‘X-type’ fields, as well as rotation measure reversals, are common features of spiral galaxies. Further observations at 3.0 GHz (S-band) as well as future scientific opportunities will also be described. Full article
(This article belongs to the Special Issue The 10th Anniversary of Galaxies: New Perspectives on Radio Surveys)
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27 pages, 14481 KiB  
Article
Porous and Ag-, Cu-, Zn-Doped Al2O3 Fabricated via Barrier Anodizing of Pure Al and Alloys
by Alexander Poznyak, Gerhard Knörnschild, Alexander Hoha and Andrei Pligovka
Coatings 2024, 14(5), 576; https://doi.org/10.3390/coatings14050576 (registering DOI) - 06 May 2024
Abstract
The paper breaks the general concepts and shows that pore formation is possible in anodic aluminum barrier oxide by anodizing of pure Al, and also presents the results of electrochemical anodizing in boric acid and citrate buffer aqueous solutions of homogeneous binary alloys [...] Read more.
The paper breaks the general concepts and shows that pore formation is possible in anodic aluminum barrier oxide by anodizing of pure Al, and also presents the results of electrochemical anodizing in boric acid and citrate buffer aqueous solutions of homogeneous binary alloys AlCu (4 wt.%), AlZn (3 wt.%) and AlAg (5.2 wt.% and 16.2 wt.%). Barrier anodizing allowed obtaining Al2O3 thin films doped with copper, zinc and silver. The anodizing behavior and the effect of anodic current density on the charge were studied, and scanning electron microscopy, X-ray photoelectron spectroscopy and Auger electron spectroscopy analyses were performed. The doped alumina thin films, which are a mixture of Al2O3, Cu2O, ZnO, Ag2O, AgO and promising double metal oxides CuAlO2, AgAlO2 and ZnAl2O4, are promising for use as resistive switching, photoelectron, mechanical, photo-thermoelectric and fluorescence materials; sensors; and transparent conductive and photocatalyst films. Full article
(This article belongs to the Section Surface Characterization, Deposition and Modification)
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19 pages, 1004 KiB  
Article
Dietary Protein Optimization for Growth and Immune Enhancement in Juvenile Hybrid Sturgeon (Acipenser baerii × A. schrenckii): Balancing Growth Performance, Serum Biochemistry, and Expression of Immune-Related Genes
by Chang’an Wang, Entong Liu, Hui Zhang, Honghe Shi, Guangwen Qiu, Shaoxia Lu, Shicheng Han, Haibo Jiang and Hongbai Liu
Biology 2024, 13(5), 324; https://doi.org/10.3390/biology13050324 (registering DOI) - 06 May 2024
Abstract
This study aimed to evaluate the effects of dietary protein levels on growth performance, serum indices, body amino acid composition, and intestinal gene expression in juvenile hybrid sturgeon (Acipenser baerii × A. schrenckii). Hybrid sturgeons (initial weight 29.21 ± 2.04 g) [...] Read more.
This study aimed to evaluate the effects of dietary protein levels on growth performance, serum indices, body amino acid composition, and intestinal gene expression in juvenile hybrid sturgeon (Acipenser baerii × A. schrenckii). Hybrid sturgeons (initial weight 29.21 ± 2.04 g) were fed isolipidic diets containing 30%, 33%, 36%, 39%, 42% or 45% crude protein for 12 weeks (n = 18 tanks, 30 fish/tank). Results showed significant differences between treatments, where weight gain and protein efficiency ratio peaked optimally between 35.9% and 38.3% dietary protein. Serum parameters such as glucose, alanine aminotransferase, aspartate aminotransferase, superoxide dismutase, and lipid peroxidation levels varied significantly with changes in dietary protein levels. Specifically, the highest enzymatic activities and growth parameters were observed in groups fed with 33% to 39% protein, enhancing whole-body concentrations of lysine, leucine, phenylalanine, proline, and glutamic acid. Immune parameters such as immunoglobulin M and lysozyme activity also showed peak levels at higher protein concentrations, particularly notable at 42% for lysozyme and 36% for both component 3 and immunoglobulin M. Gene expression related to immune and growth pathways, including MyD88, TLR1, IL-8, IL-6, NF-κB, and IL1β, was significantly upregulated at protein levels of 33% to 36%, with a noted peak in expression at 39% for TLR1, IL-10, and TOR signaling genes, before diminishing at higher protein levels. Overall, the dietary protein requirement for juvenile hybrid sturgeon ranges from 35.9% to 38.3% crude protein. Full article
21 pages, 3013 KiB  
Article
Retinoic Acid-mediated Control of Energy Metabolism is Essential for Lung Branching Morphogenesis
by Hugo Fernandes-Silva, Marco G. Alves, Marcia R. Garcez, Jorge Correia-Pinto, Pedro F. Oliveira, Catarina C. F. Homem and Rute S. Moura
Int. J. Mol. Sci. 2024, 25(9), 5054; https://doi.org/10.3390/ijms25095054 (registering DOI) - 06 May 2024
Abstract
Lung branching morphogenesis relies on intricate epithelial–mesenchymal interactions and signaling networks. Still, the interplay between signaling and energy metabolism in shaping embryonic lung development remains unexplored. Retinoic acid (RA) signaling influences lung proximal­–distal patterning and branching morphogenesis, but its role as a metabolic [...] Read more.
Lung branching morphogenesis relies on intricate epithelial–mesenchymal interactions and signaling networks. Still, the interplay between signaling and energy metabolism in shaping embryonic lung development remains unexplored. Retinoic acid (RA) signaling influences lung proximal­–distal patterning and branching morphogenesis, but its role as a metabolic modulator is unknown. Hence, this study investigates how RA signaling affects the metabolic profile of lung branching. We performed ex vivo lung explant culture of embryonic chicken lungs treated with DMSO, 1 µM RA, or 10 µM BMS493. Extracellular metabolite consumption/production was evaluated by using 1H-NMR spectroscopy. Mitochondrial respiration and biogenesis were also analyzed. Proliferation was assessed using an EdU-based assay. The expression of crucial metabolic/signaling components was examined through Western blot, qPCR, and in situ hybridization. RA signaling stimulation redirects glucose towards pyruvate and succinate production rather than to alanine or lactate. Inhibition of RA signaling reduces lung branching, resulting in a cystic-like phenotype while promoting mitochondrial function. Here, RA signaling emerges as a regulator of tissue proliferation and lactate dehydrogenase expression. Furthermore, RA governs fatty acid metabolism through an AMPK-dependent mechanism. These findings underscore RA’s pivotal role in shaping lung metabolism during branching morphogenesis, contributing to our understanding of lung development and cystic-related lung disorders. Full article
24 pages, 6122 KiB  
Article
Effects of Dietary Callicarpa nudiflora Aqueous Extract Supplementation on Growth Performance, Growth Hormone, Antioxidant and Immune Function, and Intestinal Health of Broilers
by Mengjie Liu, Gengxiong Huang, Yulin Lin, Yiwen Huang, Zhaoying Xuan, Jianchi Lun, Shiqi He, Jing Zhou, Xiaoli Chen, Qian Qu, Weijie Lv and Shining Guo
Antioxidants 2024, 13(5), 572; https://doi.org/10.3390/antiox13050572 (registering DOI) - 06 May 2024
Abstract
C. nudiflora is notably rich in flavonoids and phenylethanoid glycosides, making it a significant natural source of antioxidants. We examined the effects of C. nudiflora aqueous extract (CNE) on growth performance, antioxidant function, immunity, intestinal barrier function, nutrient transporters, and microbiota of broilers. [...] Read more.
C. nudiflora is notably rich in flavonoids and phenylethanoid glycosides, making it a significant natural source of antioxidants. We examined the effects of C. nudiflora aqueous extract (CNE) on growth performance, antioxidant function, immunity, intestinal barrier function, nutrient transporters, and microbiota of broilers. A total of 360 one-day-old broilers were randomly assigned to four treatment groups: a basal diet with 0 (control, CON), 300 mg/kg (CNEL), 500 mg/kg (CNEM), and 700 mg/kg (CNEH) CNE for 42 days. CNEL and CNEM groups quadratically increased body weight and average daily gain but decreased feed-to-gain ratios during the starter and whole phases. Regarding the immune response of broilers, CNE treatment linearly down-regulated jejunal myeloid differentiation factor 88 (MyD88) expression and interleukin-1β (IL-1β) and interferon-γ expression in the liver (d 21), while decreasing jejunal IL-1β expression and the concentration of serum tumor necrosis factor-α and interleukin-6 (d 42). The CNEM and CNEH groups had lower MyD88 and nuclear factor kappa B expression in the liver (d 21) compared to the CON group. Broilers in the CNEL and CNEM groups had higher spleen index and thymus index (d 21) and interleukin-10 expression from the liver and jejunal mucosa (d 42) than that in the CON group. For the antioxidant capacity of broilers, CNE treatment linearly decreased the content of malonaldehyde and increased the activity of total antioxidant capacity in serum (d 42). CNEM and CNEH groups linearly increased the activity of superoxide dismutase in serum and heme oxygenase-1 expression in the liver, while increasing the activity of glutathione peroxidase in serum, jejunal nuclear factor E2-related factor 2 expression, and NAD(P)H quinone oxidoreductase 1 expression in the liver (d 42). As for the growth hormone of broilers, CNEM group increased the level of serum insulin-like growth factor 1 and up-regulated jejunal glucagon-like peptide 2 (GLP-2) expression (d 21). Broilers in the CNEM and CNEH groups had higher jejunal GLP-2 expression and growth hormone (GH) expression in the liver and the level of serum GH (d 42) than that in the CON group. Additionally, the villus height and jejunal Occludin and Claudin-1 expression in the CNEM group increased. CNE-containing diets resulted in a linear increase in the expression of jejunal zonula occluden-1 (d 21), villus height to crypt depth ratio, jejunal Occludin, excitatory amino acid transporters-3, and peptide-transporter 1 (d 42). The regulation of Oscillospira, Ruminococcaceae_Ruminococcus, and Butyricicoccus genera indicated that CNEH altered the composition of the cecal microbiota. In general, supplementing broilers with C. nudiflora aqueous extract could boost hormones, immune and antioxidant function, and gut health, improving their growth performance. Hence, CNE was a promising poultry feed additive, with 500 mg/kg appearing to be the optimal dose. Full article
(This article belongs to the Special Issue Dietary Antioxidants and Animal Nutrition)
12 pages, 1828 KiB  
Article
New Understanding of Meta-Topolin Riboside Metabolism in Micropropagated Woody Plants
by Maroua Grira, Els Prinsen and Stefaan Werbrouck
Plants 2024, 13(9), 1281; https://doi.org/10.3390/plants13091281 (registering DOI) - 06 May 2024
Abstract
Topolin cytokinins have emerged as valuable tools in micropropagation. This study investigates the metabolism of meta-topolin riboside (mTR) in three distinct tree species: Handroanthus guayacan and Tabebuia rosea (Bignoniaceae), and Tectona grandis (Lamiaceae). Employing labeled N15 mTR, we [...] Read more.
Topolin cytokinins have emerged as valuable tools in micropropagation. This study investigates the metabolism of meta-topolin riboside (mTR) in three distinct tree species: Handroanthus guayacan and Tabebuia rosea (Bignoniaceae), and Tectona grandis (Lamiaceae). Employing labeled N15 mTR, we unraveled the complex mechanisms underlying cytokinin homeostasis, identifying N9-glucosylation as the principal deactivation pathway. Our findings demonstrate a capacity in T. rosea and H. guayacan to reposition the hydroxyl group on the cytokinin molecule, a previously unexplored metabolic pathway. Notably, this study reveals remarkable interfamilial and interspecies differences in mTR metabolism, challenging established perspectives on the role of callus tissue in cytokinin storage. These insights not only illuminate the metabolic intricacies of mTR, a cytokinin with interesting applications in plant tissue culture, but also enhances our understanding of cytokinin dynamics in plant systems, thereby enriching the scientific discourse on plant physiology and cytokinin biology. Full article
(This article belongs to the Special Issue Plant Propagation)
17 pages, 5048 KiB  
Article
Asymmetric Tilt-Induced Quantum Beating of Conductance Oscillation in Magnetically Modulated Dirac Matter Systems
by Nawapan Sukprasert, Patchara Rakrong, Chaiyawan Saipaopan, Wachiraporn Choopan and Watchara Liewrian
Nanomaterials 2024, 14(9), 811; https://doi.org/10.3390/nano14090811 (registering DOI) - 06 May 2024
Abstract
Herein, we investigate the effect of tilt mismatch on the quantum oscillations of spin transport properties in two-dimensional asymmetrically tilted Dirac cone systems. This study involves the examination of conductance oscillation in two distinct junction types: transverse- and longitudinal-tilted Dirac cones (TTDCs and [...] Read more.
Herein, we investigate the effect of tilt mismatch on the quantum oscillations of spin transport properties in two-dimensional asymmetrically tilted Dirac cone systems. This study involves the examination of conductance oscillation in two distinct junction types: transverse- and longitudinal-tilted Dirac cones (TTDCs and LTDCs). Our findings reveal an unusual quantum oscillation of spin-polarized conductance within the TTDC system, characterized by two distinct anomaly patterns within a single period, labeled as the linear conductance phase and the oscillatory conductance phase. Interestingly, these phases emerge in association with tilt-induced orbital pseudo-magnetization and exchange interaction. Our study also demonstrates that the structure of the LTDC can modify the frequency of spin conductance oscillation, and the asymmetric effect within this structure results in a quantum beating pattern in oscillatory spin conductance. We note that an enhancement in the asymmetric longitudinal tilt velocity ratio within the structure correspondingly amplifies the beating frequency. Our research potentially contributes valuable insights for detecting the asymmetry of tilted Dirac fermions in type-I Dirac semimetal-based spintronics and quantum devices. Full article
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22 pages, 3260 KiB  
Article
The Effects of Tremella fuciformis Polysaccharide on the Physicochemical, Multiscale Structure and Digestive Properties of Cyperus esculentus Starch
by Shanshan Zhang, Yingxu Liu, Tong Sun, Hongcheng Liu and Dawei Wang
Foods 2024, 13(9), 1425; https://doi.org/10.3390/foods13091425 (registering DOI) - 06 May 2024
Abstract
In this study, we have investigated the effects of Tremella fuciformis polysaccharide (TP) on the pasting, rheological, structural and in vitro digestive properties of Cyperus esculentus starch (CS). The results showed that the addition of TP significantly changed the pasting characteristics of CS, [...] Read more.
In this study, we have investigated the effects of Tremella fuciformis polysaccharide (TP) on the pasting, rheological, structural and in vitro digestive properties of Cyperus esculentus starch (CS). The results showed that the addition of TP significantly changed the pasting characteristics of CS, increased the pasting temperature and pasting viscosity, inhibited pasting, reduced the exudation of straight-chain starch and was positively correlated with the amount of TP added. The addition of the appropriate amount of TP could increase its apparent viscosity and enhance its viscoelasticity. The composite system of CS/TP exhibited higher short-range ordered structure and solid dense structure, which protected the crystal structure of CS, but was related to the amount of TP added. In addition, the introduction of TP not only decreased the in vitro digestion rate of CS and increased the content of slow-digestible starch (SDS) and resistant starch (RS), but also reduced the degree of digestion. Correlation studies established that TP could improve the viscoelasticity, relative crystallinity and short-range order of the CS/TP composite gel, maintain the integrity of the starch granule and crystalline structure, reduce the degree of starch pasting and strengthen the gel network structure of CS, which could help to lower the digestibility of CS. Full article
(This article belongs to the Special Issue Starch and Food Processing: Structure, Functionality and Nutrition)
16 pages, 423 KiB  
Article
Associations of eHealth Literacy with Social Activity among Community-Dwelling Older Adults: A Cross-Sectional Study
by Mamiko Eto and Koji Yamatsu
Eur. J. Investig. Health Psychol. Educ. 2024, 14(5), 1279-1294; https://doi.org/10.3390/ejihpe14050084 (registering DOI) - 06 May 2024
Abstract
Older adults who use digital technology are desired to adapt to digitalization and literacy. One required aspect is eHealth literacy, measured with the eHealth Literacy Scale (eHEALS). Many studies on eHealth literacy have used the eHEALS to examine the health behaviors of college [...] Read more.
Older adults who use digital technology are desired to adapt to digitalization and literacy. One required aspect is eHealth literacy, measured with the eHealth Literacy Scale (eHEALS). Many studies on eHealth literacy have used the eHEALS to examine the health behaviors of college students, relatively younger adults, and Internet users. However, the relevance of eHealth literacy to social activity has not yet been studied in older adults. The purposes of this study were to examine the relationship between eHealth literacy and health behaviors and social activities (community, cultural, and sports activities) and to investigate the factors associated with eHEALS scores among community-dwelling older adults. The mean eHEALS score was 12.4 points (SD 8.2), with the majority (73.3%) having the lowest score (the lowest score is 8 points). Males (17.6, SD 10.5) scored significantly higher than females (11.8, SD 7.7). The eHEALS score had a significant relationship with both cultural and community activity. Five factors significantly associated with having the lowest eHEALS score were cultural activity at least once a week, no cultural activity, no community activity, total IADL score, and intellectual activity. These results suggest that eHealth literacy is associated with community activity and cultural activity among older adults. Full article
11 pages, 1035 KiB  
Article
The Incidence of Type 2 Diabetes Mellitus and Weight Gain in People Living with HIV Receiving a Dolutegravir-Based Antiretroviral Therapy in Addis Ababa, Ethiopia: A Pilot Single-Arm Historical Cohort Study
by Tariku Shimels, Arebu Issa Bilal, Desta Samuel, Desalew Gedamu, Eden Solomon and Zewdneh Shewamene
Venereology 2024, 3(2), 96-106; https://doi.org/10.3390/venereology3020008 (registering DOI) - 06 May 2024
Abstract
Introduction: The development of antiretroviral therapy (ART) has immensely improved the quality of life of people living with HIV/AIDS. Despite such a change, concerns continue to persist regarding the safety of the latest drugs added to the regimens. This study aims to evaluate [...] Read more.
Introduction: The development of antiretroviral therapy (ART) has immensely improved the quality of life of people living with HIV/AIDS. Despite such a change, concerns continue to persist regarding the safety of the latest drugs added to the regimens. This study aims to evaluate the incidence of type 2 diabetes mellitus (T2DM) and weight gain in individuals receiving antiretroviral therapy containing dolutegravir at a general hospital in Addis Ababa, Ethiopia. Methods: A retrospective cohort study was conducted at RDDMH from 1 February to 30 March 2022. The study included PLHIV who had dolutegravir substituted into their combined regimen in November 2019. Collected data underwent cleaning, entry, and analysis using Statistical Package for Social Sciences (SPSS) v. 26.0 and R programing. Descriptive statistics were employed for univariate and bivariate analysis. The Kaplan–Meier model in R was used to illustrate the hazard function. A significance level of p < 0.05 and a 95% confidence interval were employed for statistical reporting. Results: The study followed 185 PLHIV who were on ART who either substituted their previous regimens or initiated a new dolutegravir-based regimen for 12 months. Most were females (59.5%), aged over 38 years (57.5%), married (50.8%), and had lived with HIV for 7 or more years (51.9%). The incidence proportion of T2DM in this sample was 7.0% (95% CI: 3.8–10.3). The age category (X2(1, N = 185) = 12.29, p < 0.001) exhibited a statistically significant relationship with the incidence of T2DM. The cumulative rate of T2DM in the age group over 38 years was approximately 15.4%. The pairwise Wilcoxon signed rank test revealed statistically significant differences in BMI scores between time points. Conclusion: This study observed a noteworthy incidence of T2DM among PLHIV receiving a dolutegravir-based first-line ART. Healthcare providers should prioritize early follow-up and management options for PLHIV who are on dolutegravir-based ART regimens. Full article
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19 pages, 10853 KiB  
Article
Microstructure and Phase Composition of Novel Crossover Al-Zn-Mg-Cu-Zr-Y(Er) Alloys with Equal Zn/Mg/Cu Ratio and Cr Addition
by Maria V. Glavatskikh, Ruslan Yu. Barkov, Leonid E. Gorlov, Maxim G. Khomutov and Andrey V. Pozdniakov
Metals 2024, 14(5), 547; https://doi.org/10.3390/met14050547 (registering DOI) - 06 May 2024
Abstract
The effect of 0.2%Cr addition on the structure, phase composition, and mechanical properties of the novel cast and wrought Al-2.5Zn-2.5Mg-2.5Cu-0.2Zr-Er(Y) alloys were investigated in detail. Chromium is distributed between primary crystals (5.7–6.8%) of the intermetallic phase and the aluminum solid solution (0.2%) (Al). [...] Read more.
The effect of 0.2%Cr addition on the structure, phase composition, and mechanical properties of the novel cast and wrought Al-2.5Zn-2.5Mg-2.5Cu-0.2Zr-Er(Y) alloys were investigated in detail. Chromium is distributed between primary crystals (5.7–6.8%) of the intermetallic phase and the aluminum solid solution (0.2%) (Al). The primary crystals contain for the main part Cr, Ti, Er(Y). The experimental phase composition is in good correlation with the thermodynamic computation data. The micron-sized solidification origin phases (Al8Cu4Er(or Y) and Mg2Si) and supersaturated (Al) with nano-sized Al3(Zr,Ti) and E (Al18Mg3Cr2) precipitates are presented in the microstructure of the novel alloys after solution treatment. The nucleation of η (MgZn2) (0.5%), S (Al2CuMg) (0.4%), and T (Al,Zn,Mg,Cu) (8.8%) phase precipitates at 180 °С, providing the achievement of a maximum hardness of 135 HV in the Al2.5Zn2.5Mg2.5CuYCr alloy. The corrosion potential of the novel alloy is similar to the Ecor of the referenced alloy, but the corrosion current density (0.68–0.98 µA/sm2) is still significantly lower due to the formation of E (Al18Mg3Cr2) precipitates and S phase precipitates of the aging origin, in addition to the T phase. The formation of E (Al18Mg3Cr2) precipitates under the solution treatment provides a lower proportion of recrystallized grains (2.5–5% vs. 22.4–25.1%) and higher hardness (110 HV vs. 85–95 HV) in the Cr-rich alloys compared to the referenced alloys. Solution treated, hot and cold rolled, recrystallized, water quenched and aged at 210 °С alloys demonstrate an excellent microstructure stability and tensile properties: YS = 299–300 MPa, UTS = 406–414 MPa, and El. = 9–12.3%. Full article
27 pages, 985 KiB  
Article
Probability Distributions for Modeling Stock Market Returns—An Empirical Inquiry
by Jayanta K. Pokharel, Gokarna Aryal, Netra Khanal and Chris P. Tsokos
Int. J. Financial Stud. 2024, 12(2), 43; https://doi.org/10.3390/ijfs12020043 (registering DOI) - 06 May 2024
Abstract
Investing in stocks and shares is a common strategy to pursue potential gains while considering future financial needs, such as retirement and children’s education. Effectively managing investment risk requires thoroughly analyzing stock market returns and making informed predictions. Traditional models often utilize normal [...] Read more.
Investing in stocks and shares is a common strategy to pursue potential gains while considering future financial needs, such as retirement and children’s education. Effectively managing investment risk requires thoroughly analyzing stock market returns and making informed predictions. Traditional models often utilize normal variance distributions to describe these returns. However, stock market returns often deviate from normality, exhibiting skewness, higher kurtosis, heavier tails, and a more pronounced center. This paper investigates the Laplace distribution and its generalized forms, including asymmetric Laplace, skewed Laplace, and the Kumaraswamy Laplace distribution, for modeling stock market returns. Our analysis involves a comparative study with the widely-used Variance-Gamma distribution, assessing their fit with the weekly returns of the S&P 500 Index and its eleven business sectors, drawing parallel inferences from international stock market indices like IBOVESPA and KOSPI for emerging and developed economies, as well as the 20+ Years Treasury Bond ETFs and individual stocks across varied time horizons. The empirical findings indicate the superior performance of the Kumaraswamy Laplace distribution, which establishes it as a robust alternative for precise return predictions and efficient risk mitigation in investments. Full article
18 pages, 769 KiB  
Article
How Credible Is the 25-Year Photovoltaic (PV) Performance Warranty?—A Techno-Financial Evaluation and Implications for the Sustainable Development of the PV Industry
by Pao-Hsiang Hsi and Joseph C. P. Shieh
Sustainability 2024, 16(9), 3880; https://doi.org/10.3390/su16093880 (registering DOI) - 06 May 2024
Abstract
To support the bankability of PV projects, PV manufacturers have been offering some of the longest warranties in the world, typically in the range of 25–30 years. During the warranty period, PV manufacturers guarantee that the degradation of PV modules will not exceed [...] Read more.
To support the bankability of PV projects, PV manufacturers have been offering some of the longest warranties in the world, typically in the range of 25–30 years. During the warranty period, PV manufacturers guarantee that the degradation of PV modules will not exceed 0.4%-0.6% each year, or the buyer can at any time make a claim to the manufacturer for replacement or compensation for the shortfall. Due to PV popularity, the performance warranty terms have become more and more competitive each year. However, long-term PV operating data are very limited, and the bankruptcy of PV manufacturers has been quite common. Without a proper methodology to assess the adequacy of the warranty fund (WF) reserves of PV manufacturers, the 25-year performance warranty can reflect an empty promise. To ensure sustainable development of the PV industry, this study develops a probability-weighted expected value method to determine the necessary WF reserve based on benchmark field degradation data and a prevailing degradation cap of 0.55% per year. The simulation results show that, unless the manufacturer’s degradation pattern is significantly better than the benchmark degradation profile, 1.302% of the sales value is required for the WF reserve. To the best of our knowledge, this is the first study that provides WF reserve requirement estimation for 25-year PV performance warranties. The results will provide transparency for PV investors and motivation for PV manufacturers to engage in continuous quality improvement, as all such achievements can now be reflected in the annual report results of manufacturers. Full article
(This article belongs to the Special Issue Solar Energy Utilization and Sustainable Development)
15 pages, 309 KiB  
Article
A Compact Model for the Clustered Orienteering Problem
by Roberto Montemanni and Derek H. Smith
Logistics 2024, 8(2), 48; https://doi.org/10.3390/logistics8020048 (registering DOI) - 06 May 2024
Abstract
Background: The Clustered Orienteering Problem is an optimization problem faced in last-mile logistics. The aim is, given an available time window, to visit vertices and to collect as much profit as possible in the given time. The vertices to visit have to be [...] Read more.
Background: The Clustered Orienteering Problem is an optimization problem faced in last-mile logistics. The aim is, given an available time window, to visit vertices and to collect as much profit as possible in the given time. The vertices to visit have to be selected among a set of service requests. In particular, the vertices belong to clusters, the profits are associated with clusters, and the price relative to a cluster is collected only if all the vertices of a cluster are visited. Any solving methods providing better solutions also imply a new step towards sustainable logistics since companies can rely on more efficient delivery patterns, which, in turn, are associated with an improved urban environment with benefits both to the population and the administration thanks to an optimized and controlled last-mile delivery flow. Methods: In this paper, we propose a constraint programming model for the problem, and we empirically evaluate the potential of the new model by solving it with out-of-the-box software. Results: The results indicate that, when compared to the exact methods currently available in the literature, the new approach proposed stands out. Moreover, when comparing the quality of the heuristic solutions retrieved by the new model with those found by tailored methods, a good performance can be observed. In more detail, many new best-known upper bounds for the cost of the optimal solutions are reported, and several instances are solved to optimality for the first time. Conclusions: The paper provides a new practical and easy-to-implement tool to effectively deal with an optimization problem commonly faced in last-mile logistics. Full article
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