DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation. In this study, the muscle development-related gene POLB was screened by selection signature and RNA-seq analysis and then validated for the proliferation and apoptosis of bovine primary myocytes. It was also found that overexpression of the POLB gene had a pro-apoptosis effect, but interfering with the expression of the gene had no significant effect on cells. Then, the analysis of related apoptotic genes revealed that POLB overexpression affected CASP9 gene expression. Full article

In East Asia, where concentrated summer precipitation often leads to climate disasters, understanding the factors that cause such extreme rainfall is crucial for effective forecasting and preparedness. The western North Pacific subtropical high (WNPSH) is a key driver of summer precipitation variability, and therefore, its monitoring is critical to predicting the wet or dry periods during the East Asian summer monsoon. Using the Geo-KOMPSAT 2A (GK2A) satellite cloud amount data and ERA5 reanalysis data during the years 2020–2023, this study identified three leading empirical orthogonal function (EOF) modes and investigated the associated WNPSH variability at synoptic and subseasonal scales. The analysis includes a linear regression of meteorological fields onto the principal component (PC) time series. All three modes play a role in the spatiotemporal variability of the WNPSH, exhibiting lead–lag relationships. In particular, the second mode is responsible for its northwestward shift and intensification. As the WNPSH moves northwestward, the position of the monsoon rain band also shifts, and its intensity is modulated mainly by the moisture transport along the WNPSH boundary. Our results highlight the potential of high-resolution, real-time data from the GK2A satellite to elucidate WNPSH variability and its impact on the East Asian summer monsoon. By addressing the variability of the WNSPH using GK2A data, we pave the way for the development of a real-time monitoring framework with GK2A, which will improve our predictability and readiness for extreme weather events in East Asia. Full article

This research investigates how the Lahore Metrobus system (MBS) influences perceptions of urban space quality through the lens of urban design and transport infrastructure. Lahore is undergoing significant urban changes with the introduction of a newly constructed mass transit system, which thus necessitates a joint development strategy to preserve its cultural heritage. Recent evolutions in transport planning has heightened the interest in analyzing the way mobility factors affect the perceptions of a place’s quality. The gap in previous work lies in the limited focus on qualitative, human-centered perspectives regarding the impacts of public projects like the MBS on urban space quality. This study uniquely fills this void by examining the influence of the MBS on people’s lives and the quality of urban spaces. Comparing pre- and post-Metrobus scenarios, along with questionnaire surveys of riders and shopkeepers, exposes the neglect of the social image during design and construction. The system was implemented with a focus solely on hard, core infrastructure, thereby neglecting soft components such as area development, social and cultural value, and human-centered design. The government should unite transport agencies, stakeholders, and the public to craft a joint policy for enhancing revenue, ridership, and fostering transit-oriented development (TOD). Research findings will help in achieving social and cultural sustainability for upcoming transport lines in Pakistan, as well as directions for other developing nations looking to implement mass transit networks. Full article

Metallodrugs are an important group of medicinal agents used for the treatment of various diseases ranging from cancers to viral, bacterial, and parasitic diseases. Their distinctive features include the availability of a metal centre, redox activity, as well as the ability to multitarget. Diruthenium paddlewheel complexes are an intensely developing group of metal scaffolds, which can securely coordinate bidentate xenobiotics and transport them to target tissues, releasing them by means of substitution reactions with biomolecular nucleophiles. It is of the utmost importance to gain a complete comprehension of which chemical reactions happen with them in physiological milieu to design novel drugs based on these bimetallic scaffolds. This review presents the data obtained in experiments and calculations, which clarify the chemistry these complexes undergo once administered in the proteic environment. This study demonstrates how diruthenium paddlewheel complexes may indeed embody a new paradigm in the design of metal-based drugs of dual-action by presenting and discussing the protein metalation by these complexes. Full article

Quantum physics is intrinsically probabilistic, where the Born rule yields the probabilities associated with a state that deterministically evolves. The entropy of a quantum state quantifies the amount of randomness (or information loss) of such a state. The degrees of freedom of a quantum state are position and spin. We focus on the spin degree of freedom and elucidate the spin-entropy. Then, we present some of its properties and show how entanglement increases spin-entropy. A dynamic model for the time evolution of spin-entropy concludes the paper. Full article

The dietary patterns of older adults, particularly in relation to meat, egg, and dairy (MED) consumption, significantly impact frailty, a state of heightened vulnerability to adverse health outcomes. This paper investigates the association between MED consumption and frailty among older Chinese adults, considering rural/urban disparities and gender differences. Analyzing data from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) spanning from 2008 to 2018, this study explores how MED consumption influences frailty levels over time. The results show that moderate MED consumption is associated with slower frailty progression, suggesting a protective effect against frailty among older adults. However, excessive MED consumption, particularly among rural residents and females, is linked to accelerated frailty progression. Urban residents and males report higher MED consumption levels, possibly due to their greater access to diverse food options and traditional dietary preferences. The findings underscore the complex interplay between dietary habits, demographic factors, and frailty outcomes. Understanding these dynamics is crucial for developing targeted interventions to mitigate frailty risk factors and promote healthy aging among Chinese older adults. Full article

The study aims to improve the quality of wooden breast meat (WBM) via the use of newly developed marinades based on selected strains of lactic acid bacteria (LAB) in combination with the by-products of the dairy and fruit/berry industries. Six distinct marinades were produced based on milk permeate (MP) fermented with Lacticaseibacillus casei (Lc) and Liquorilactobacillus uvarum (Lu) with the addition of apple (ApBp) and blackcurrant (BcBp) processing by-products. The microbiological and acidity parameters of the fermented marinades were evaluated. The effects of marinades on the microbiological, technical, and physicochemical properties of meat were assessed following 24 and 48 h of WBM treatment. It was established that LAB viable counts in marinades were higher than 7.00 log₁₀ colony-forming units (CFU)/mL and, after 48 h of marination, enterobacteria and molds/yeasts in WBM were absent. Marinated (24 and 48 h) WBM showed lower dry-matter and protein content, as well as water holding capacity, and exhibited higher drip loss (by 8.76%) and cooking loss (by 12.3%) in comparison with controls. After WBM treatment, biogenic amines decreased; besides, the absence of spermidine and phenylethylamine was observed in meat marinated for 48 h with a marinade prepared with Lu. Overall, this study highlights the potential advantages of the developed sustainable marinades in enhancing the safety and quality attributes of WBM. Full article

With abundant information and interconnectedness among people, identifying knowledgeable individuals in specific domains has become crucial for organizations. Artificial intelligence (AI) algorithms have been employed to evaluate the knowledge and locate experts in specific areas, alleviating the manual burden of expert profiling and identification. However, there is a limited body of research exploring the application of AI algorithms for expert finding in the medical and biomedical fields. This study aims to conduct a scoping review of existing literature on utilizing AI algorithms for expert identification in medical domains. We systematically searched five platforms using a customized search string, and 21 studies were identified through other sources. The search spanned studies up to 2023, and study eligibility and selection adhered to the PRISMA 2020 statement. A total of 571 studies were assessed from the search. Out of these, we included six studies conducted between 2014 and 2020 that met our review criteria. Four studies used a machine learning algorithm as their model, while two utilized natural language processing. One study combined both approaches. All six studies demonstrated significant success in expert retrieval compared to baseline algorithms, as measured by various scoring metrics. AI enhances expert finding accuracy and effectiveness. However, more work is needed in intelligent medical expert retrieval. Full article

Livestock management is challenging for resource-poor (R-P) farmers due to unavailability of quality feed, limited professional advice, and rumor-spreading about animal health condition in a herd. This research seeks to improve animal health in southern Africa by promoting sericea lespedeza (Lespedeza cuneata), a nutraceutical forage legume. An automated geospatial model for precision agriculture (PA) can identify suitable locations for its cultivation. Additionally, a novel approach of radio-frequency identifier (RFID) supported telemetry technology can track animal movement, and the analyses of data using artificial intelligence can determine sickness of small ruminants. This RFID-based system is being connected to a smartphone app (under construction) to alert farmers of potential livestock health issues in real time so they can take immediate corrective measures. An accompanying Decision Support System (DSS) site is being developed for R-P farmers to obtain all possible support on livestock production, including the designed PA and RFID-based DSS. Full article

The cloning of resistance-related genes CsROP5/CsROP10 and the analysis of their mechanism of action provide a theoretical basis for the development of molecular breeding of disease-resistant cucumbers. The structure domains of two Rho-related guanosine triphosphatases from plant (ROP) genes were systematically analyzed using the bioinformatics method in cucumber plants, and the genes CsROP5 (Cucsa.322750) and CsROP10 (Cucsa.197080) were cloned. The functions of the two genes were analyzed using reverse-transcription quantitative PCR (RT-qPCR), virus-induced gene silencing (VIGS), transient overexpression, cucumber genetic transformation, and histochemical staining technology. The conserved elements of the CsROP5/CsROP10 proteins include five sequence motifs (G1-G5), a recognition site for serine/threonine kinases, and a hypervariable region (HVR). The knockdown of CsROP10 through VIGS affected the transcript levels of ABA-signaling-pathway-related genes (CsPYL, CsPP2Cs, CsSnRK2s, and CsABI5), ROS-signaling-pathway-related genes (CsRBOHD and CsRBOHF), and defense-related genes (CsPR2 and CsPR3), thereby improving cucumber resistance to Corynespora cassiicola. Meanwhile, inhibiting the expression of CsROP5 regulated the expression levels of ROS-signaling-pathway-related genes (CsRBOHD and CsRBOHF) and defense-related genes (CsPR2 and CsPR3), thereby enhancing the resistance of cucumber to C. cassiicola. Overall, CsROP5 and CsROP10 may participate in cucumber resistance to C. cassiicola through the ROS and ABA signaling pathways. Full article

Background: Cognitive impairment (CI) is a common mental health disorder among older adults, and dietary patterns have an impact on cognitive function. However, no systematic researches have constructed anti-inflammatory diet (AID) and protein-enriched diet (PED) to explore their association with CI among older adults in China. Methods: The data used in this study were obtained from the 2018 waves of the China Longitudinal Health and Longevity Survey (CLHLS). We construct AID, PED, and calculate scores for CI. We use binary logistic regression to explore the relationship between them, and use restrictive cubic splines to determine whether the relationships are non-linear. Subgroup analysis and sensitivity analysis were used to demonstrate the robustness of the results. Results: A total of 8692 participants (mean age is 83.53 years) were included in the analysis. We found that participants with a higher AID (OR = 0.789, 95% confidence interval: 0.740–0.842, p < 0.001) and PED (OR = 0.910, 95% confidence interval: 0.866–0.956, p < 0.001) score showed lower odds of suffering from CI. Besides, the relationship between the two dietary patterns and CI is linear, and the results of subgroup analysis and sensitivity analysis are also significant. Conclusion: Higher intakes of AID and PED are associated with a lower risk of CI among older adults, which has important implications for future prevention and control of CI from a dietary and nutritional perspective. Full article

Smoke tree (Cotinus coggygria) is an important ornamental tree that represents the autumnal landscape of red leaves in Northern China, especially in Beijing. However, Verticillium wilt, caused by the fungus (Verticillium dahliae), has resulted in a high mortality rate for smoke trees, posing a serious threat to the highly valued landscape of red leaves in Beijing. To explore an efficient control measure for Verticillium wilt, we systematically analyzed the applicability and efficacy of multiple treatments for three consecutive years in Xiangshan Park and Badaling Forest Park. From 2021 to 2023, diseased smoke trees in Xiangshan Park were subjected to three application methods (agent irrigation, trunk injection, or a combination of the two) and five candidate agents, namely Bacillus subtilis, azoxystrobin, propiconazole, carbendazim, and prochloraz. Analyses of the data for three consecutive years revealed a decreasing trend in the annual disease incidence rate. Specifically, the combined application of agent irrigation and trunk injection exhibited the highest control effect and a significant improvement in the landscape of red leaves in Beijing. Furthermore, the combination of propiconazole via irrigation plus the trunk injection of carbendazim and prochloraz had the greatest control effect. These suppressive measurements were further used and demonstrated to be effective in Badaling Forest Park. Overall, our study provides an effective disease management means for controlling Verticillium wilt in smoke trees. Full article

Actinomycetes have long been recognized as an important source of antibacterial natural products. In recent years, actinomycetes in extreme environments have become one of the main research directions. Streptomyces sp. KN37 was isolated from the cold region of Kanas in Xinjiang. It demonstrated potent antimicrobial activity, but the primary active compounds remained unclear. Therefore, we aimed to combine genomics with traditional isolation methods to obtain bioactive compounds from the strain KN37. Whole-genome sequencing and KEGG enrichment analysis indicated that KN37 possesses the potential for synthesizing secondary metabolites, and 41 biosynthetic gene clusters were predicted, some of which showed high similarity to known gene clusters responsible for the biosynthesis of antimicrobial antibiotics. The traditional isolation methods and activity-guided fractionation were employed to isolate and purify seven compounds with strong bioactivity from the fermentation broth of the strain KN37. These compounds were identified as 4-(Diethylamino)salicylaldehyde (1), 4-Nitrosodiphenylamine (2), N-(2,4-Dimethylphenyl)formamide (3), 4-Nitrocatechol (4), Methylsuccinic acid (5), Phenyllactic acid (6) and 5,6-Dimethylbenzimidazole (7). Moreover, 4-(Diethylamino)salicylaldehyde exhibited the most potent inhibitory effect against Rhizoctonia solani, with an EC₅₀ value of 14.487 mg/L, while 4-Nitrosodiphenylamine showed great antibacterial activity against Erwinia amylovora, with an EC₅₀ value of 5.715 mg/L. This study successfully isolated several highly active antimicrobial compounds from the metabolites of the strain KN37, which could contribute as scaffolds for subsequent chemical synthesis. On the other hand, the newly predicted antibiotic-like substances have not yet been isolated, but they still hold significant research value. They are instructive in the study of active natural product biosynthetic pathways, activation of silent gene clusters, and engineering bacteria construction. Full article

The tourism industry has recently undergone a significant evolution because of the implementation of Information and Communication Technologies (ICT). These changes have raised new conceptions of destinations, thus giving rise to smart destinations. In this context, digital tracking of tourists has become of great interest. This article aims to analyze the research landscape on tourist tracking through a bibliometric study, which allows the evaluation of the performance of publications, recognition of the prominent SciVal topics, analysis of the evolution of the techniques, and recognition of their use as a tool that contributes to the development of smart destinations. Unlike prior studies in this area, in addition to the performance analysis of publications, this paper focuses only on empirical studies on tourist tracking, identifies the prominent SciVal topics, and analyzes the linkage thereof with the Sustainable Development Goals (SDGs). The bibliometric study was carried out based on articles on tourist tracking extracted from the Scopus database and published between 2007 and 2023. This research is mainly quantitative, based on research performance analysis (journals, authors, institutions, and countries) and science mapping (citation analysis, co-word analysis, co-authorship analysis). Results show the quality of the publications made in the 2007–2023 period and indicate that the most used techniques have been GPS devices, social media, and app-based GPS/GNSS. In addition, it was found that the evolution of the techniques occurred at the same time as the development of ICT and that the prominent SciVal topic that covers the largest number of publications is associated with destination management. It is concluded that the application of tourist tracking techniques contributes to the transformation of destinations into smart destinations, as they provide useful relevant information to tourism market stakeholders for the development of strategies that improve decision-making and allow for the sustainable management of destinations. Full article

A series of flavanols were synthesized to assess their biological activity against human non-small cell lung cancer cells (A549). Among the sixteen synthesized compounds, it was observed that compounds 6k (3.14 ± 0.29 µM) and 6l (0.46 ± 0.02 µM) exhibited higher potency compared to 5-fluorouracil (5-Fu, 4.98 ± 0.41 µM), a clinical anticancer drug which was used as a positive control. Moreover, compound 6l (4’-bromoflavonol) markedly induced apoptosis of A549 cells through the mitochondrial- and caspase-3-dependent pathways. Consequently, compound 6l might be developed as a candidate for treating or preventing lung cancer. Full article

Specific environmental characteristics can encourage active leisure travel and increase physical activity. However, existing environment-travel studies tend to ignore the differences in environmental characteristics associated with route choice and travel distance, of which the latter could be more important for health benefits, since longer trips are associated with increased exercise. Additionally, the most recent studies focus on leisure walking and leisure cycling, and activities such as hiking, climbing, and running are examined less frequently. This study, therefore, compares the similarities and differences of the environmental factors associated with route selection and travel distance through non-parametric tests and Cox proportional hazard models. The results show that two intersecting sets of environmental elements relate to both the route chosen and the distance traveled. Land use diversity and varied topography are appealing for both leisure trips and trip length. In addition, the differences in environmental characteristics among specific leisure travels may be attributed to variations in physical activity requirements, preferences for landscape viewing, and/or sensitivity to crowding. Therefore, conclusions drawn without considering the different types of leisure travel could be skewed. Whether particular surroundings may effectively increase physical activity remains uncertain. A more holistic perspective could be beneficial when studying the connection between the environment, active travel, and health. Full article

The Sustainable Time-Dependent Cheapest Path Problem (STDCPP) entails locating a Hamiltonian path that covers all of the graph’s vertices at the lowest possible total sustainability cost. The issue is inspired by actual city logistics, where it is important to consider the opinions of diverse stakeholders in the light of sustainable urban mobility plans and service viability. To address this issue, this paper suggests a twofold contribution. First, we describe the Sustainable Time-Dependent Cheapest Path Problem and define the complex cost function, which, based on the multi-criteria decision-making approach, integrates the views of different stakeholders and sustainability elements into the route cost calculation. Second, we show that the modified problem satisfies the FIFO (First-In First-Out) property and demonstrate the applicability of the suggested approach on a real-life scenario where route sustainability is extracted from the traffic sign information system available in Flanders, Belgium. Full article

This study sought to explore the antimicrobial activity of punicalagin against V. parahaemolyticus and its potential modes of action. V. parahaemolyticus ATCC 17802 and RIMD 2210633^Sm were exposed to punicalagin, and the energy production, membrane potential, and envelope permeability, as well as the interaction with cell biomolecules, were measured using a variety of fluorescent probes combined with electrophoresis and Raman spectroscopy. Punicalagin treatment disrupted the envelope integrity and induced a decrease in intracellular ATP and pH. The uptake of 1-N-phenyl-naphtylamine (NPN) demonstrated that punicalagin weakened the outer membrane. Punicalagin damaged the cytoplasmic membrane, as indicated by the membrane depolarization and the leakage of intracellular potassium ions, proteins, and nucleic acids. Electronic microscopy observation visualized the cell damage caused by punicalagin. Further, gel electrophoresis coupled with the Raman spectrum assay revealed that punicalagin affected the protein expression of V. parahaemolyticus, and there was no effect on the integrity of genomic DNA. Therefore, the cell envelope and proteins of V. parahaemolyticus were the assailable targets of punicalagin treatment. These findings suggested that punicalagin may be promising as a natural bacteriostatic agent to control the growth of V. parahaemolyticus. Full article

With the rapid growth in the number of EVs, a huge number of EVs are connected to the power grid for charging, which places a great amount of pressure on the stable operation of the power grid. This paper focuses on the development of V2G applications, based on the current research status of V2G technology. Firstly, the standards on V2G applications and some pilot projects involving more representative V2G systems are introduced. Comparing V2G applications with ordered charging and unordered charging, the social and economic benefits of V2G applications are highlighted. Analysis of the social benefits of V2G applications concerns three points: the grid demand response, personalized charging, and the coordination of renewable energy sources. And analysis of the economic benefits of V2G applications is divided into three parties: the grid, the aggregator, and individuals. From the perspective of innovative EVs expanding the application scenarios through V2G technology, V2G applications for commercial EVs, emergency power applications, and vehicle-to-vehicle energy trading are introduced. The current challenges related to V2G applications are presented: users’ willingness to participate in V2G applications, battery loss, charging and discharging tariffs, privacy and security, and power loss. Finally, some research recommendations for the development of V2G applications are given and the current state of research in regard to those recommendations is presented. Full article

A cesarean scar ectopic pregnancy (CSP) represents an uncommon yet potentially life-threatening condition requiring immediate and efficient management. We present a case of a 32-year-old woman diagnosed with a scar pregnancy at 8 weeks of gestation. Laparoscopic surgical management was chosen due to its minimally invasive nature and potential for preserving fertility. During the procedure, temporary clipping of uterine arteries was employed to control intraoperative bleeding. The patient recovered well postoperatively with no complications. This case highlights the feasibility and effectiveness of laparoscopic intervention combined with temporary uterine artery clipping in the management of scar pregnancies, offering a valuable approach for clinicians faced with similar cases. Through this report, we aim to contribute to the existing literature on the optimal management of CSP and highlight the efficacy of laparoscopic surgery in this context. Full article

Rabies is a fatal encephalitic infectious disease caused by the rabies virus (RABV). RABV is highly neurotropic and replicates in neuronal cell lines in vitro. The RABV fixed strain, HEP-Flury, was produced via passaging in primary chicken embryonic fibroblast cells. HEP-Flury showed rapid adaptation when propagated in mouse neuroblastoma (MNA) cells. In this study, we compared the growth of our previously constructed recombinant HEP (rHEP) strain—based on the sequence of the HEP (HEP-Flury) strain—with that of the original HEP strain. The original HEP strain exhibited higher titer than rHEP and a single substitution at position 80 in the matrix (M) protein M(D80N) after incubation in MNA cells, which was absent in rHEP. In vivo, intracerebral inoculation of the rHEP-M(D80N) strain with this substitution resulted in enhanced viral growth in the mouse brain and a significant loss of body weight in the adult mice. The number of viral antigen-positive cells in the brains of adult mice inoculated with the rHEP-M(D80N) strain was significantly higher than that with the rHEP strain at 5 days post-inoculation. Our findings demonstrate that a single amino acid substitution in the M protein M(D80N) is associated with neurovirulence in mice owing to adaptation to mouse neuronal cells. Full article

Background: Aeromonas hydrophila is a widely recognized broad-spectrum pathogen that primarily targets the gastrointestinal tract. Type IV pili (T4P) are proteinaceous nano-machines located on the bacterial cell surface, playing a crucial role in host colonization and infection. Regrettably, the T4P systems of A. hydrophila remain largely underexplored. Methods: A. hydrophila genomes with complete genome assembly and annotation reports up to March 31, 2023, were obtained from the NCBI Genome database or KEGG genome database, followed by a global search for T4P secretion system genes. Protein sequences of these manually curetted genes were used as secondary quarry for Synteny analysis. Protein–protein interaction analysis was performed by string analysis and in silico study of genomic islands. Results: We identified 27 orthologs of type IV pili (T4P) nano-machine components in A. hydrophila. These orthologs are primarily distributed across three operons: pilABCD, pilMNOPQ, and pilVWXY. While the first two operons are commonly found in all experimental genomes, the presence of the pilVWXY operon, coding for 11 orthologs, is reported here for the first time in A. hydrophila. Notably, the complete pilVWXY operon is absent in nonvirulent strains. A genomic islands study between a nonvirulent and hypervirulent strain also confirms absence of most of the genes coded by pilVWXY in nonvirulent strain. Interestingly, among the 51 experimental genomes analyzed, the pilVWXY operon was completely absent in 10 strains, most of which are categorized as nonvirulent; Conclusions: The distribution of two major type IV pili (T4P) nano-machines, PilABCDMNOPQ and PilVWXY, is reported here for the first time in A. hydrophila. Additionally, this study suggests a potential role for the PilVWXY nano-machine in establishing human disease. Full article