Cryptococcal infection poses a significant global public health challenge, particularly in regions near the equator. In this review, we offer a succinct exploration of the Cryptococcus spp. genome and various molecular typing methods to assess the burden and genetic diversity of cryptococcal pathogens in the environment and clinical isolates. We delve into a detailed discussion on the molecular pathogenesis and diagnosis of immune reconstitution inflammatory syndrome (IRIS) associated with cryptococcosis, with a specific emphasis on cryptococcal meningitis IRIS (CM-IRIS). Our examination includes the recent literature on CM-IRIS, covering host cellulomics, proteomics, transcriptomics, and genomics. Full article

This paper investigates the effects of high-frequency switching and a high fundamental frequency on the parameters and efficiency of a high-speed permanent magnet synchronous machine (PMSM) drive. We discuss the design and modeling of the PMSM, taking into account these high-frequency effects. The impact of high frequencies is analyzed across three different inverters (IGBT, Fast IGBT, and SiC-MOSFET) and the motor, and we employ theoretical analysis, computer simulations, and experimental tests for validation. Our goal is to enhance our understanding of how these high-frequency factors affect the performance of the motor drive. Full article

The construction industry has been identified as a major contributor to occupational accidents that can lead to fatalities. As a result, this study aims to evaluate the effectiveness of new safety and health regulations and revised guidelines in improving safety and health disclosures and performance within the construction industry. We retrieved safety and health disclosure reports from 25 Taiwanese construction companies between 2013 and 2020 using the Market Observation Post System website. We analyzed the data using the Kaplan–Meier method to assess the timing of disclosures and differences between larger (≥300 employees) and smaller (<300 employees) companies. We found that construction companies reported safety indicators more promptly than health indicators and that larger companies disclosed earlier compared to smaller ones. Only 45% of companies provide detailed reviews and preventative measures in their sustainability reports despite 64% disclosing occupational accidents. We found that from 2013 to 2020, more companies improved their occupational safety and health (OSH) reporting. This improvement coincided significantly with the adoption of international standards and Taiwan’s government regulations. In summary, the study found that larger companies were more likely to disclose OSH data compared to smaller ones. This suggests that company size and available resources could have an impact on reporting practices. While some progress was made, companies still struggle to provide detailed reports on major accidents, balancing transparency with competitiveness. Full article

Multiwave interference plays a crucial role in shaping the spatial variations of internal tides. Based on a combination of in situ mooring and altimeter data, interference of semidiurnal internal tides was investigated in the northern South China Sea. Mooring observations indicate the observed kinetic-to-potential energy ratio and group speed are both relatively lower than the theoretical values of mode-1 semidiurnal internal tides, indicating the presence of partly-standing waves. This is consistent with the altimeter result that the mooring was located at the antinode within the interference pattern formed by the superposition of the westward and southward semidiurnal internal tides from the Luzon Strait and the continental slope of the southern Taiwan Strait. However, the kinetic-to-potential energy ratio and group velocity were notably changed when an anticyclonic eddy passed by the mooring. By employing the ray-tracing method, we identified that mesoscale processes may induce a phase difference in the semidiurnal internal tides between the Luzon Strait and the continental slope of the southern Taiwan Strait. This alteration further leads to changes in the positions of nodes and antinodes within the interference pattern of the semidiurnal internal tides. Full article

This paper explores the domain of intelligent transportation systems, specifically focusing on roundabouts as potential solutions in the context of smart mobility. Roundabouts offer a safer and more efficient driving environment compared to other intersections, thanks to their curvilinear trajectories promoting speed control and lower vehicular speeds for traffic calming. The synthesis review supported the authors in presenting current knowledge and emerging needs in roundabout design and evaluation. A focused examination of the models and methods used to assess safety and operational performance of roundabout systems was necessary. This is particularly relevant in light of new challenges posed by the automotive market and the influence of vehicle-to-vehicle communication on the conceptualization and design of this road infrastructure. Two case studies of roundabouts were analyzed in Aimsun to simulate the increasing market penetration rates of connected and autonomous vehicles (CAVs) and their traffic impacts. Through microscopic traffic simulation, the research evaluated safety and performance efficiency advancements in roundabouts. The paper concludes by outlining areas for further research and evolving perspectives on the role of roundabouts in the transition toward connected and autonomous vehicles and infrastructures. Full article

Hyper-spectral imaging (HSI) systems can be divided into two main types as follows: a group of systems that includes a dedicated dispersion/filtering component whose role is to physically separate the different wavelengths and a group of systems that sample all wavelengths in parallel, so that the separation into wavelengths is performed by signal processing (interferometric method). There is a significant advantage to systems of the second type in terms of the integration time required to obtain a signal with a high signal-to-noise ratio since the signal-to-noise ratio of methods based on scanning interferometry (Windowing method) is better compared to methods based on dispersion. The current research deals with the feasibility study of a new concept for an HSI system that is based on scanning interferometry using the “push-broom” method. In this study, we investigated the viability of incorporating a simple birefringent plate into a scanning optical system. By exploiting the motion of the platform on which the system is mounted, we extracted the spectral information of the scanned region. This approach combines the benefits of scanning interferometry with the simplicity of the setup. According to the theory, a chirped cosine-shaped interferogram is obtained for each wavelength due to the nonlinear behavior of the optical path difference of light in the birefringent plate as a function of the angle. An algorithm converts the signal from a superposition of chirped cosine signals to a scaled interferogram such that Fourier transforming (FT) the interferogram retrieves the spectral information. This innovative idea can turn a simple monochrome camera into a hyperspectral camera by adding a relief lens and a birefringent plate. Full article

One of the main barriers to the successful treatment of laryngeal squamous cell carcinoma (LSCC) is postoperative progression, primarily due to tumor cell metastasis. To systematically investigate the molecular characteristics and potential mechanisms underlying the metastasis in laryngeal cancer, we carried out a TMT-based proteomic analysis of both cancerous and adjacent non-cancerous tissues from 10 LSCC patients with lymph node metastasis (LNM) and 10 without. A total of 5545 proteins were quantified across all samples. We identified 57 proteins that were downregulated in LSCC with LNM, which were enriched in cell adhesion pathways, and 69 upregulated proteins predominantly enriched in protein production pathways. Importantly, our data revealed a strong correlation between increased ribosomal activity and the presence of LNM, as 18 ribosomal subunit proteins were found to be upregulated, with RPS10 and RPL24 being the most significantly overexpressed. The potential of ribosomal proteins, including RPS10 and RPL24, as biomarkers for LSCC with LNM was confirmed in external validation samples (six with LNM and six without LNM) using Western blotting and immunohistochemistry. Furthermore, we have confirmed that the RNA polymerase I inhibitor CX-5461, which impedes ribosome biogenesis in LSCC, also decreases the expression of RPS10, RPL24, and RPS26. In vitro experiments have revealed that CX-5461 moderately reduces cell viability, while it significantly inhibits the invasion and migration of LSCC cells. It can enhance the expression of the epithelial marker CDH1 and suppress the expression of the mesenchymal markers CDH2, VIM, and FN at a dose that does not affect cell viability. Our study broadens the scope of the proteomic data on laryngeal cancer and suggests that ribosome targeting could be a supplementary therapeutic strategy for metastatic LSCC. Full article

Blackcurrant (Ribes nigrum L.) is a berry bush widely cultivated in Europe for producing juices, jams, jellies, and syrups. In addition to berries, blackcurrant leaves and pomace, as byproducts, have also been shown to have health-promoting effects. Static digestion, simulating oral, gastric, and small intestinal digestion, was applied, and blackcurrant leaves, fruits, and pomace and the polyphenol bioaccessibility were evaluated in terms of recovery index. The results were related to sample type, and the recovery index presented higher values in the case of fruits, indicating this morphological part of blackcurrant as the most bioaccessible. The antioxidant potential of blackcurrant was evaluated using four different methods, with the leaves proving to be a significant and powerful antioxidant compared to fruits and pomace. The counteracting potential of inhibiting the oxidation process was evaluated using in vitro-induced lipid peroxidation and the inhibition potential of superoxide and hydroxyl anions. The antioxidant evaluation and the inhibition of biological and non-biological radicals indicate the leaf extract is the most powerful antioxidant studied. Also, the results proved that not only fruits but also the blackcurrant byproducts (pomace and leaves) are promising sources of bioaccessible antioxidants with potential benefits in animal nutrition. Full article

Logistics companies are increasingly adopting private blockchains for enhanced data management because of the trends in cooperation. Nevertheless, this practice poses new challenges concerning the security and sharing of data. The real-time nature and diversity of logistics data increase the difficulty of protecting the data. Additionally, when transportation information changes, downstream enterprises must promptly adjust their production plans to accommodate these alterations. The strict access controls of private blockchains can obstruct downstream enterprises from obtaining data, posing a challenge to the overall operational efficiency. In this paper, we propose an innovative logistics data protection scheme that employs private set intersection (PSI) and blockchain cross-chain technology to achieve data security. In our scheme, logistics companies within the logistics consortium are added as trusted agents to the public blockchain, enabling downstream enterprises to acquire logistics data integration from the public blockchain. Utilizing an RSA-based PSI protocol, our approach enhances exchange efficiency while protecting private data without transmitting additional information. We evaluate the performance of the proposed solution through a series of experiments, and the results demonstrate that our solution can achieve secure and efficient logistics data exchange. Full article

Based on several clinical observations it was hypothesized that herpesviruses may influence the replication of human bocaviruses, the second known parvoviruses that have been confirmed as human pathogens. While several cell lines support the growth of HSV-1, HBoV-1 was exclusively cultivated on air–liquid interface cultures, the latter being a rather complicated, slow, and low throughput system. One of the cell lines are T84 cells, which are derived from the lung metastasis of a colorectal tumor. In this study, we provide evidence that T84 also supports HBoV replication when cultivated as monolayers, while simultaneously being permissive for HSV-1. The cell culture model thus would enable co-infection studies of both viruses and is worth being optimized for high throughput studies with HBoV-1. Additionally, the study provides evidence for a supporting effect of HSV-1 on the replication and packaging of HBoV-1 progeny DNA into DNase-resistant viral particles. Full article

The emergence of digital mobility services holds great promise for enhancing efficiency, convenience, and accessibility for passengers. However, these benefits are predominantly accessible to those proficient in utilizing these technologies, which may intensify the disparity in transportation usage. This paper presents plans to alleviate the digital divide in the mobility sector. First, two fundamental approaches were established through a literature review: (1) app usage education and (2) an AI-based Mobility Service App. To substantiate the effectiveness of these approaches, a pilot test was conducted in Seoul. The results of the pilot test showed that the AI-based Mobility Service App was effective for reducing travel time and enhancing the convenience of passage. Accordingly, the Technology Acceptance Model was adopted to derive technology acceptance factors of the AI-based Mobility Service App. Finally, a phased approach with short-term, medium-term, and long-term plans was proposed based on the analysis results to ensure sustainable policies in the mobility sector. Full article

The National Institute for Health and Care Excellence (NICE) in England uses quality-adjusted life years (QALYs) to assess the cost-effectiveness of treatments. A QALY is a measure that combines the size of the clinical benefit of a treatment with the time the patient benefits from it, i.e., the time horizon. We wanted to know how consistently QALY gains are calculated at NICE. Therefore, we have analysed information on the time horizons used for the QALY calculations of the concluded evaluations conducted under the Highly Specialised Technologies programme for treatments of very rare diseases at NICE. For treatments with final guidance published by December 2023 (n = 29), a time horizon of median 97.5 years (range: 35 to 125 years) was used to calculate the QALY gains. For most QALY calculations, the accepted time horizon was longer than either the expected treatment duration or the estimated life expectancy. In contrast, for the only technology with a final negative funding decision, i.e., afamelanotide for treating the lifelong chronic disease erythropoietic protoporphyria, a time horizon that was shorter than the expected treatment duration was used. The fairness and consistency of the evaluation process of treatments for very rare diseases at NICE should be reviewed. Full article

Human dihydrofolate reductase (hDHFR) is an essential cellular enzyme, and inhibiting its activity is a promising strategy for cancer therapy. We have chosen the trimethoprim molecule (TMP) as a model compound in our search for a new class of hDHFR inhibitors. We incorporated an amide bond, a structural element typical of netropsin, a ligand that binds selectively in the minor groove of DNA, into the molecules of TMP analogs. In this work, we present previously obtained and evaluated eleven benzamides (JW1–JW8; MB1, MB3-MB4). Recently, these compounds were specifically projected as potential inhibitors of the enzymes acetylcholinesterase (AChE) and β-secretase (BACE1). JW8 was most active against AChE, with an inhibitory concentration of AChE IC₅₀ = 0.056 µM, while the IC₅₀ for donepezil was 0.046 µM. This compound was also the most active against the BACE1 enzyme. The IC₅₀ value was 9.01 µM compared to that for quercetin, with IC₅₀ = 4.89 µM. All the benzamides were active against hDHFR, with IC₅₀ values ranging from 4.72 to 20.17 µM, and showed activity greater than TMP (55.26 µM). Quantitative results identified the derivatives JW2 and JW8 as the most promising. A molecular modeling study demonstrates that JW2 interacts strongly with the key residue Gly-117, while JW8 interacts strongly with Asn-64 and Arg-70. Furthermore, JW2 and JW8 demonstrate the ability to stabilize the hDHFR enzyme, despite forming fewer hydrogen bonds with the protein compared to reference ligands. It can be concluded that this class of compounds certainly holds great promise for good active leads in medicinal chemistry. Full article

Acute myocardial infarction (AMI) is one of the leading causes of death worldwide. Cell apoptosis in the myocardium plays an important role in ischemia and reperfusion (I/R) injury, leading to cardiac damage and dysfunction. Platelets are major players in hemostasis and play a crucial role in vessel occlusion, inflammation, and cardiac remodeling after I/R. Here, we studied the impact of platelets on cell apoptosis in the myocardium using a close-chest mouse model of AMI. We found caspase-3-positive resident cardiac cells, while leukocytes were negative for caspase-3. Using two different mouse models of thrombocytopenia, we detected a significant reduction in caspase-3 positive cells in the infarct border zone after I/R injury. Further, we identified platelet FasL to induce cell apoptosis via the extrinsic pathway of Fas receptor activation of target cells. Mechanistically, hypoxia triggers platelet adhesion to FasR, suggesting that platelet-induced apoptosis is elevated after I/R. Platelet-specific FasL knock-out mice showed reduced Bax and Bcl2 expression, suggesting that platelets modulate the intrinsic and extrinsic pathways of apoptosis, leading to reduced infarct size after myocardial I/R injury. Thus, a new mechanism for how platelets contribute to tissue homeostasis after AMI was identified that should be validated in patients soon. Full article

Once thought of as an immune-privileged site, we now know that the nervous system communicates in a bidirectional manner with the immune system via the neuroimmune axis. Neuropeptides constitute a component of this axis, playing critical roles in the brain and periphery. The function of salivary neuropeptides in the acute stress response is not well understood. The purpose of this study is to investigate salivary neuropeptide levels during acute stress. Salivary samples were collected from fire recruits engaged in a stress training exercise previously shown to induce acute stress, at three separate timepoints during the exercise and levels of oxytocin, neurotensin, Substance P, α-MSH, and β-Endorphin were measured using the Human Neuropeptide 5-Plex Custom Assay Eve Technologies. All neuropeptides increased throughout the acute stress simulation and during the recovery phase. Exploratory factor analysis (EFA) identified one factor contributing to baseline values across five neuropeptides and Pairwise Pearson Correlation Coefficient analysis showed positive correlations >0.9 for almost all neuropeptide combinations at the pre-stress timepoint. Further analysis identified negative and positive correlations between past-life trauma and self-assessed hardiness, respectively. Calculated neuropeptide scores showed an overall positive correlation to self-assessed hardiness. Altogether, our results suggest that salivary neuropeptides increase synchronously during acute stress and higher levels correlate with an increase in self-assessed hardiness. Further study is required to determine if interventions designed to enhance neuropeptide activity can increase stress resilience, especially in high-stress occupations such as firefighting. Full article

Nutrient pollution—mainly nitrogen and phosphorus—caused by organic waste continues to impact the environment. The implementation of a circular economy is integral to alleviating these effects. Liquid digestate, which is a byproduct of anaerobic digestion (a waste-valorising process), is a nutrient-dense organic fertiliser with vast applications in agriculture. Using an aerobic polishing unit, this study developed a viable method for the preparation of a hydroponic fertiliser by investigating the effect of pH on the nutrient recycling capabilities of said system. The heterotrophic bacteria present in the biofilm, identified by 16S gene sequencing, are responsible for 90% of organic carbon (as TOC) removal with minimal ammonium loss. This is ideal for promoting optimal nitrification in hydroponic systems in the absence of organic carbon to ensure plant growth is not affected. Although pH 8 was found to be ideal for batch operation, this pH condition resulted in decreased microbial longevity and, therefore, increased ammonification due to microbial decay. Therefore, continuous operation at pH 7 proved to be a better option owing to the ammonium-rich effluent (>220 mg/L) which was produced, which is on par with the nitrogen concentration of a Hoagland solution. The continuous carbon polishing of liquid digestate provides an efficient way of utilising organic fertilisers in hydroponic systems. Full article

The number of failures serves as a critical indicator that dynamically impacts the reliability of self-announcing failure products, making it highly practical to incorporate the failure count into reliability management throughout the entire product life cycle. This paper investigates comprehensive methodologies for effectively managing the reliability of self-announcing failure products throughout both the warranty and post-warranty stages, taking into account factors such as the failure count, mission cycles, and limited time duration. Three renewable warranty strategies are introduced alongside proposed models for post-warranty replacements. By analyzing variables like the failure number, mission cycles, and time constraints, these proposed warranties provide practical frameworks for efficient reliability management during the warranty stage. Additionally, the introduced warranties utilize cost and time metrics to extract valuable insights that inform decision making and enable effective reliability management during the warranty stage. Moreover, this study establishes cost and time metrics for key post-warranty replacements, facilitating the development of individual cost rates and model applications in other post-warranty scenarios. Analyses of the renewable free-repair–replacement warranties demonstrate that establishing an appropriate number of failures as the replacement threshold can effectively reduce warranty-servicing costs and extend the coverage duration. Full article

The aim of the study was to evaluate whether the static stabilometric parameters among elite fencers, were affected by prolonged, asymmetric training regimen. A sample of 26 elite fencers of both genders, aged 19.15 ± 2.24 years, practising one of the three disciplines foil, épée, or sabre, was recruited for the study. Anthropometric measurements including thigh and calf circumferences and postural assessment based on the weight distribution on a stabilimeter platform were performed. Postural tone, as indicated by measures such as sway length and sway area ratio was calculated.. No notable anthropometric asymmetries were detected within the examined group The weight distribution patterns on the support quadrants in static stabilometric measurements did not suggest clinically significant issues. There were no significant differences among subgroups based on gender and lower limb dominance for both anthropometric and stabilimeter variables. However, 30.8% of participants showed anomalies in postural tone (hypertonic and hypotonic condition). Five out of eight athletes found with abnormal postural tone were foil fencers, suggesting a potential discipline-specific effect. Individual adjustments were found in foil fencers. These findings provide insights into the potential effects of fencing training on postural parameters among elite athletes. Full article

Using beneficial microorganisms, such as purple non-sulfur bacteria (PNSB), has shown enormous potential for improving plant growth and agricultural production. However, the full extent of their benefits and interactions with agricultural practices is yet to be fully understood. The present study aimed to investigate the use of PNSB in crop rotation practice, focusing on its impact on rice growth and yield. The experiment was conducted over two rice cropping seasons, with djulis grown between the rice as a rotation crop. The study shows that PNSB treatment increased the concentration of 5-aminolevulinic acid (5-ALA) in plants, indicating enhanced photosynthesis. Moreover, when combined with crop rotation, PNSB remarkably improved soil fertility. These combined benefits resulted in substantial increases in tiller numbers (163%), leaf chlorophyll content (13%), and lodging resistance (66%), compared to the untreated plants. The combined treatment also resulted in higher productive tillers per hill (112%), average grain per hill (65%), and grain fertility (26%). This led to increased grain yield (65%), shoot dry weight (15%), and harvest index (37%). The findings clearly suggest that the incorporation of PNSB in crop rotation strategies can significantly augment the growth and yield of rice crops. These insights, pivotal for sustainable rice cultivation, hold the potential to simultaneously tackle the pressing issues of global food security and climate change. Full article

Objectives: In this study, we investigated the antitumor immunomodulatory effects of rapamycin in oral cancer. Study Design: We examined the proliferation, apoptosis, and migration of cancer cells and investigated the cell surface expression levels of immune accessory molecules and T cell immune responses in vitro. We investigated the effect of in vivo administration of rapamycin on immune cell distribution and T cell immune responses in oral tumor-bearing mice. Results: Rapamycin treatment significantly inhibited OSCC cell proliferation and migration, increased apoptotic cell death, and upregulated cell surface expression of several immune accessory and adhesion molecules, including CD40, CD83, PD-L1, PD-L2, MHC class I, P-selectin, and VCAM-1. These cancer cells augmented T cell proliferation. In vivo rapamycin administration significantly attenuated mouse tumor growth with an increased proportion of immune cells, including CD4⁺ T cells, CD8⁺ T cells, and dendritic cells (DCs); decreased the proportion of immune suppressive cells, such as myeloid-derived suppressor cells and regulatory T cells; enhanced DC maturation and upregulated the surface expression of CD40, CD86, and ICAM-1. Conclusions: Our results suggest that the therapeutic effect of mTOR inhibition in oral cancer can cause direct antitumor and immunomodulatory effects. Full article

Aims: The current review aims to outline and summarize the latest research on aflatoxin, with research studies describing natural, herbal and chemical compound applications in animal (pig) models and in vitro cellular studies. Aflatoxin, a carcinogenic toxin metabolite, is produced by Aspergillus flavus in humid environments, posing a threat to human health and crop production. The current treatment involves the prevention of exposure to aflatoxin and counteracting its harmful toxic effects, enabling survival and research studies on an antidote for aflatoxin. Objectives: To summarize current research prospects and to outline the influence of aflatoxin on animal forage in farm production, food and crop processing. The research application of remedies to treat aflatoxin is undergoing development to pinpoint biochemical pathways responsible for aflatoxin effects transmission and actions of treatment. Significance: To underline the environmental stress of aflatoxin on meat and dairy products; to describe clinical syndromes associated with aflatoxicosis on human health that are counteracted with proposed treatment and preventive interventions. To understand how to improve the health of farm animals with feed conditions. Full article

Psoriasis is a systemic autoimmune/autoinflammatory disease that can be well studied in established mouse models. Skin-resident macrophages are classified into epidermal Langerhans cells and dermal macrophages and are involved in innate immunity, orchestration of adaptive immunity, and maintenance of tissue homeostasis due to their ability to constantly shift their phenotype and adapt to the current microenvironment. Consequently, both macrophage populations play dual roles in psoriasis. In some circumstances, pro-inflammatory activated macrophages and Langerhans cells trigger psoriatic inflammation, while in other cases their anti-inflammatory stimulation results in amelioration of the disease. These features make macrophages interesting candidates for modern therapeutic strategies. Owing to the significant progress in knowledge, our review article summarizes current achievements and indicates future research directions to better understand the function of macrophages in psoriasis. Full article