The 2023 MDPI Annual Report has
been released!
 
17 pages, 3181 KiB  
Article
Development of Noninvasive Method for the Automated Analysis of Nine Steroid Hormones in Human Saliva by Online Coupling of In-Tube Solid-Phase Microextraction with Liquid Chromatography–Tandem Mass Spectrometry
by Takashi Hitomi and Hiroyuki Kataoka
Analytica 2024, 5(2), 233-249; https://doi.org/10.3390/analytica5020015 (registering DOI) - 9 May 2024
Abstract
Accurate measurement of steroid hormones is crucial to elucidate new mechanisms of action and diagnose steroid metabolism-related diseases. This study presents a simple, sensitive, and automated analytical method for nine representative steroid hormones. The method involves on-line coupling of in-tube solid-phase microextraction (IT-SPME) [...] Read more.
Accurate measurement of steroid hormones is crucial to elucidate new mechanisms of action and diagnose steroid metabolism-related diseases. This study presents a simple, sensitive, and automated analytical method for nine representative steroid hormones. The method involves on-line coupling of in-tube solid-phase microextraction (IT-SPME) with liquid chromatography–tandem mass spectrometry (LC–MS/MS). The steroid hormones were extracted and enriched on a Supel-Q PLOT capillary column using IT-SPME. Subsequently, they were separated and detected within 6 min using a Discovery HS F5-3 column and positive ion mode multiple reaction monitoring system via LC–MS/MS. Calibration curves of these compounds using each stable isotope-labeled internal standard (IS) showed linearity with correlation coefficients greater than 0.9990 in the range of 0.01–40 ng/mL, with limits of detection (S/N = 3) of 0.7–21 pg/mL. Moreover, intra- and inter-day variations were lower than 8.1 and 15% (n = 6), respectively. The recoveries of these compounds from saliva samples were in the range of 82–114%. The developed IT-SPME/LC–MS/MS method of steroid hormones is a highly sensitive, specific, and non-invasive analytical method that allows extraction and enrichment with no organic solvents, and enables direct automated online analysis by simply ultrafiltrating a small sample of saliva. Full article
(This article belongs to the Section Sample Pretreatment and Extraction)
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10 pages, 939 KiB  
Article
Inkjet Printing of Long-Range Ordering Two-Dimensional Magnetic Ti0.8Co0.2O2 Film
by Yuntian Du and Pengxiang Zhang
Nanomaterials 2024, 14(10), 834; https://doi.org/10.3390/nano14100834 (registering DOI) - 9 May 2024
Abstract
The value of two-dimensional (2D) materials in printed electronics has been gradually explored, and the rheological properties of 2D material dispersions are very different for various printing technologies. Understanding the rheological properties of 2D material dispersions plays a vital role in selecting the [...] Read more.
The value of two-dimensional (2D) materials in printed electronics has been gradually explored, and the rheological properties of 2D material dispersions are very different for various printing technologies. Understanding the rheological properties of 2D material dispersions plays a vital role in selecting the optimal manufacturing technology. Inkjet printing is suitable for small nanosheet sizes and low solution viscosity, and it has a significant advantage in developing nanosheet inks because of its masklessness, high efficiency, and high precision. In this work, we selected 2D Ti0.8Co0.2O2 nanosheets, which can be synthesized in large quantities by the liquid phase exfoliation technique; investigated the effects of nanosheet particle size, solution concentration on the rheological properties of the dispersion; and obtained the optimal printing processing method of the dispersion as inkjet printing. The ultrathin Ti0.8Co0.2O2 nanosheet films were prepared by inkjet printing, and their magnetic characteristics were compared with those of Ti0.8Co0.2O2 powder. The films prepared by inkjet printing exhibited long-range ordering, maintaining the nanosheet powders’ paramagnetic characteristics. Our work underscored the potential of inkjet printing as a promising method for fabricating precisely controlled thin films using 2D materials, with applications spanning electronics, sensors, and catalysis. Full article
(This article belongs to the Special Issue Nanomaterials for Flexible and Printed Electronics)
18 pages, 383 KiB  
Article
An Ad Hoc Procedure for Testing Serial Correlation in Spatial Fixed-Effects Panels
by Giovanni Millo
Mathematics 2024, 12(10), 1475; https://doi.org/10.3390/math12101475 (registering DOI) - 9 May 2024
Abstract
We consider testing for error persistence in spatial panels with (potentially correlated) individual heterogeneity. We propose two variants of an ad hoc testing procedure based on first transforming out the individual effects, either by time-demeaning or by taking forward orthogonal deviations, then estimating [...] Read more.
We consider testing for error persistence in spatial panels with (potentially correlated) individual heterogeneity. We propose two variants of an ad hoc testing procedure based on first transforming out the individual effects, either by time-demeaning or by taking forward orthogonal deviations, then estimating an encompassing spatio-temporal model. The procedure can also be employed under the random effects assumption, in which case, although suboptimal, it can be computationally cheaper and safer than existing tests. We report Monte Carlo simulations demonstrating satisfactory empirical properties. Full article
(This article belongs to the Special Issue Mathematical Economics and Spatial Econometrics)
22 pages, 596 KiB  
Review
Unlocking the Potential of Mannosylerythritol Lipids: Properties and Industrial Applications
by Joana Dias de Almeida, Miguel Figueiredo Nascimento, Petar Keković, Frederico Castelo Ferreira and Nuno Torres Faria
Fermentation 2024, 10(5), 246; https://doi.org/10.3390/fermentation10050246 (registering DOI) - 9 May 2024
Abstract
Mannosylerythritol lipids (MELs), one of the most promising biosurfactants (BS), are glycolipids produced by yeasts or fungi, which have great environmental performance and high compatibility with the human body. MELs, besides working as typical surfactants, can form diverse structures when at or above [...] Read more.
Mannosylerythritol lipids (MELs), one of the most promising biosurfactants (BS), are glycolipids produced by yeasts or fungi, which have great environmental performance and high compatibility with the human body. MELs, besides working as typical surfactants, can form diverse structures when at or above the critical aggregation concentration (CAC), reduce the surface tension of water and other solutions, and be stable over a wide range of conditions. Among others, MELs present antimicrobial, antitumor, antioxidant and anti-inflammatory activities and skin and hair repair capacity, which opens possibilities for their use in applications from cosmetics and pharmaceutics to bioremediation and agriculture. However, their market share is still low when compared to other glycolipids, due to their less developed production process and higher production cost. This review gathers information on the potential applications of MELs mentioned in the literature since 1993. Furthermore, it also explores the current strategies being developed to enhance the market presence of MELs, in parallel with the ones developed for rhamnolipids and sophorolipids. Full article
26 pages, 2275 KiB  
Review
Transient Left Ventricular Dysfunction from Cardiomyopathies to Myocardial Viability: When and Why Cardiac Function Recovers
by Giancarlo Trimarchi, Lucio Teresi, Roberto Licordari, Alessandro Pingitore, Fausto Pizzino, Patrizia Grimaldi, Danila Calabrò, Paolo Liotta, Antonio Micari, Cesare de Gregorio and Gianluca Di Bella
Biomedicines 2024, 12(5), 1051; https://doi.org/10.3390/biomedicines12051051 (registering DOI) - 9 May 2024
Abstract
Transient left ventricular dysfunction (TLVD), a temporary condition marked by reversible impairment of ventricular function, remains an underdiagnosed yet significant contributor to morbidity and mortality in clinical practice. Unlike the well-explored atherosclerotic disease of the epicardial coronary arteries, the diverse etiologies of TLVD [...] Read more.
Transient left ventricular dysfunction (TLVD), a temporary condition marked by reversible impairment of ventricular function, remains an underdiagnosed yet significant contributor to morbidity and mortality in clinical practice. Unlike the well-explored atherosclerotic disease of the epicardial coronary arteries, the diverse etiologies of TLVD require greater attention for proper diagnosis and management. The spectrum of disorders associated with TLVD includes stress-induced cardiomyopathy, central nervous system injuries, histaminergic syndromes, various inflammatory diseases, pregnancy-related conditions, and genetically determined syndromes. Furthermore, myocardial infarction with non-obstructive coronary arteries (MINOCA) origins such as coronary artery spasm, coronary thromboembolism, and spontaneous coronary artery dissection (SCAD) may also manifest as TLVD, eventually showing recovery. This review highlights the range of ischemic and non-ischemic clinical situations that lead to TLVD, gathering conditions like Tako-Tsubo Syndrome (TTS), Kounis syndrome (KS), Myocarditis, Peripartum Cardiomyopathy (PPCM), and Tachycardia-induced cardiomyopathy (TIC). Differentiation amongst these causes is crucial, as they involve distinct clinical, instrumental, and genetic predictors that bode different outcomes and recovery potential for left ventricular function. The purpose of this review is to improve everyday clinical approaches to treating these diseases by providing an extensive survey of conditions linked with TLVD and the elements impacting prognosis and outcomes. Full article
(This article belongs to the Special Issue Cardiomyopathies and Heart Failure: Charting the Future)
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21 pages, 1377 KiB  
Review
Energy Compensation for Crop Growth under Plastic Mulching: Theories, Models, and Limitations
by Dianyuan Ding, Ting Li, Lihong Wu, Xi Zhang, Ying Zhao, Hao Feng, Chao Zhang and Ole Wendroth
Agronomy 2024, 14(5), 1005; https://doi.org/10.3390/agronomy14051005 (registering DOI) - 9 May 2024
Abstract
Plastic film mulching (PM) is a useful agronomic means to adapt to the environmental conditions of dryland agriculture and improve crop production. To improve the theoretical framework of PM technology, this study focuses on the interaction between the soil temperature change caused by [...] Read more.
Plastic film mulching (PM) is a useful agronomic means to adapt to the environmental conditions of dryland agriculture and improve crop production. To improve the theoretical framework of PM technology, this study focuses on the interaction between the soil temperature change caused by PM and crop growth. The definition, action mechanism, and simulation of the compensatory effect of PM on growing degree days are introduced to reveal the effect of soil temperature under PM on crop development and growth. Our summary shows that the strength of the warming effect changes with the growth and development of crops, strengthening during the early stage of crop growth and gradually weakening as a crop canopy develops. Generally, the warming effect has a good promotion effect on crop growth, but the crop growth is hampered even with a yield reduction when the increased soil temperature caused by PM exceeds the tolerant temperature for plant growth. Moreover, the compensatory effect of PM could be used to quantify the growth and development of crops under PM and has been widely applied to cotton, corn, winter wheat, and rice. The compensation coefficient is larger in the early stage of crop growth than in the later stage. The compensation coefficient has certain differences for the same crop because of the influence of climate factors, soil moisture content, and soil microtopography. In future research, the theoretical integration of the safety period of PM and the time threshold of the compensatory effect could be theoretically interpreted, and the construction of the compensatory effect module in the crop models will also be an important issue. Full article
(This article belongs to the Special Issue The Impact of Mulching on Crop Production and Farmland Environment)
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18 pages, 310 KiB  
Article
Serum Levels of Zinc, Albumin, Interleukin-6 and CRP in Patients with Unipolar and Bipolar Depression: Cross Sectional Study
by Tihana Bagarić, Alma Mihaljević-Peleš, Milena Skočić Hanžek, Maja Živković, Ana Kozmar and Dunja Rogić
Curr. Issues Mol. Biol. 2024, 46(5), 4533-4550; https://doi.org/10.3390/cimb46050275 (registering DOI) - 9 May 2024
Abstract
Unipolar (UD) and bipolar depression (BDD) show a high degree of similarity in clinical presentations, which complicates the differential diagnosis of these disorders. The aim of this study was to investigate the serum levels of interleukin 6 (IL-6), C-reactive protein (CRP), albumin (Alb), [...] Read more.
Unipolar (UD) and bipolar depression (BDD) show a high degree of similarity in clinical presentations, which complicates the differential diagnosis of these disorders. The aim of this study was to investigate the serum levels of interleukin 6 (IL-6), C-reactive protein (CRP), albumin (Alb), and zinc (Zn) in patients with UD, BDD, and healthy controls (HC). A total of 211 samples were collected: 131 patient samples (65 UD and 68 BDD) and 80 HC. The Montgomery–Asberg Depression Rating Scale (MADRS), along with the Hamilton Depression Rating Scale (HAMD-17), were administered to patient groups to evaluate symptoms. A cross-sectional study was performed to analyse the serum levels of IL-6, CRP, albumin, and zinc. The concentration of CRP was determined using the immunoturbidimetry method, zinc using the colorimetric method, and albumin using the colorimetric method with bromocresol green on the Alinity c device. IL-6 cytokine concentration in serum samples was ascertained using a commercial enzyme immunoassay, ELISA. We found no significant differences in serum concentrations of zinc, albumin, CRP, and IL-6 between the groups of patients with unipolar and bipolar depression. There was a significant statistical difference (p < 0.001) between serum levels of all investigated parameters in both groups of depressed patients in comparison with HC. Furthermore, correlations with specific items on HAMD-17; (namely, hypochondrias, work and activities, somatic symptoms-general, and weight loss) and on MADRS (concentration difficulties, lassitude) were observed in both patient groups. These findings confirm the presence of low-grade inflammation in depression, thus adding better insight into the inflammation hypothesis directed to explain the aetiology of depressive disorders. Our results do not indicate potential biomarkers for distinguishing between unipolar and bipolar depression. Full article
(This article belongs to the Special Issue Molecules at Play in Neurological Diseases 2024)
13 pages, 537 KiB  
Article
Hourly Feeding Regime of Modern Genetics Lactating Sows: Enhancing Productive Performance, Welfare, and Piglet Growth in Smart Farm-Based Systems
by Keiven Mark B. Ampode, Hong-Seok Mun, Eddiemar B. Lagua, Veasna Chem, Hae-Rang Park, Young-Hwa Kim, Md Sharifuzzaman, Md Kamrul Hasan and Chul-Ju Yang
Agriculture 2024, 14(5), 740; https://doi.org/10.3390/agriculture14050740 (registering DOI) - 9 May 2024
Abstract
Effective management of lactating sows significantly influences various aspects of swine production. This study compared the impact of an hourly feeding regime and a five-times-daily feeding regime on the productive performance, body condition, and welfare of lactating sows, as well as on the [...] Read more.
Effective management of lactating sows significantly influences various aspects of swine production. This study compared the impact of an hourly feeding regime and a five-times-daily feeding regime on the productive performance, body condition, and welfare of lactating sows, as well as on the growth performance of their offspring. Twenty-eight lactating sows (Landrace × Yorkshire) were divided into two groups: Group 1 was fed five times a day, and Group 2 was fed according to an hourly regime. The data were analyzed using independent-samples T-tests and the Mann–Whitney U test using Statistical Analysis System (SAS, 2011, Version 9.3) software. An hourly feeding regime positively affected (p < 0.05) sows’ feed intake and body condition, significantly reducing the days from the weaning-to-estrus interval. Group 1 exhibited significantly higher (p < 0.05) reductions in backfat thickness (BFT) and body condition score (BCS) during the weaning period compared to Group 2. Additionally, significant differences (p < 0.05) were observed in regard to sow body weight loss, feed intake, piglet livability and mortality rate at weaning, sow index, and calculated milk yield. Feeding sows according to an hourly regime positively impacted their productive performance compared to those fed five times daily. No significant differences (p > 0.05) were recorded in regard to the total number of piglets born, live births, mummified piglets, stillbirths, piglet mortality, litter size at weaning, and sow feed conversion ratio (FCR). However, the number of piglets weaned per sow per year (PSY) was numerically higher in Group 2 (p > 0.05). The piglets from Group 2 had significantly higher (p < 0.05) weaning weights and exhibited lower feed intake, greater weight gain, improved average daily gain, and greater litter size weight gain than those from Group 1. Statistically, sows from Group 2 exhibited a higher frequency of standing (p < 0.05), which potentially contributed to the reduction in shoulder skin lesions in sows (p > 0.05). In conclusion, an hourly feeding regime could optimize sow productive performance, body condition, milk yield, welfare, and piglet growth in swine production. Full article
(This article belongs to the Special Issue Improvements of Reproduction and Growth Performance in Pig Farming)
6 pages, 239 KiB  
Editorial
Introduction: Border Politics and Refugee Narratives in Contemporary Literature
by Laura Lojo-Rodríguez and Noemí Pereira-Ares
Humanities 2024, 13(3), 74; https://doi.org/10.3390/h13030074 (registering DOI) - 9 May 2024
Abstract
Refugeehood—whether triggered by religious intolerance, ethnic strife, political repression, war, or environmental factors—has been a constant throughout human history, but it was not until the twentieth century that refugees were endowed with legal status on an international level [...] Full article
(This article belongs to the Special Issue Border Politics & Refugee Narratives in Contemporary Literature)
15 pages, 1580 KiB  
Article
Design of Lumbar Rehabilitation Training System Based on Virtual Reality
by Jiani Liu, Ping Shi and Hongliu Yu
Electronics 2024, 13(10), 1850; https://doi.org/10.3390/electronics13101850 (registering DOI) - 9 May 2024
Abstract
A virtual reality-based lumbar rehabilitation training system is designed to address the increasing number of patients with low back pain (LBP) year by year. Attitude sensors are used to track lower back movement. In order to improve the effect of rehabilitation training, several [...] Read more.
A virtual reality-based lumbar rehabilitation training system is designed to address the increasing number of patients with low back pain (LBP) year by year. Attitude sensors are used to track lower back movement. In order to improve the effect of rehabilitation training, several virtual rehabilitation training games and assessment scenes are designed based on the Unity3D engine to complete different tasks from simple to complex. The goal is to increase patients’ interest in rehabilitation training. The experimental results verify the accuracy of rehabilitation data acquisition, real-time interactive communication, and the smooth operation of rehabilitation scenes. Full article
20 pages, 3556 KiB  
Article
Solvent Exsolution and Liberation from Different Heavy Oil–Solvent Systems in Bulk Phases and Porous Media: A Comparison Study
by Wei Zou and Yongan Gu
Energies 2024, 17(10), 2287; https://doi.org/10.3390/en17102287 (registering DOI) - 9 May 2024
Abstract
In this paper, experimental and numerical studies were conducted to differentiate solvent exsolution and liberation processes from different heavy oil–solvent systems in bulk phases and porous media. Experimentally, two series of constant-composition-expansion (CCE) tests in a PVT cell and differential fluid production (DFP) [...] Read more.
In this paper, experimental and numerical studies were conducted to differentiate solvent exsolution and liberation processes from different heavy oil–solvent systems in bulk phases and porous media. Experimentally, two series of constant-composition-expansion (CCE) tests in a PVT cell and differential fluid production (DFP) tests in a sandpacked model were performed and compared in the heavy oil–CO2, heavy oil–CH4, and heavy oil–C3H8 systems. The experimental results showed that the solvent exsolution from each heavy oil–solvent system in the porous media occurred at a higher pressure. The measured bubble-nucleation pressures (Pn) of the heavy oil–CO2 system, heavy oil–CH4 system, and heavy oil–C3H8 system in the porous media were 0.24 MPa, 0.90 MPa, and 0.02 MPa higher than those in the bulk phases, respectively. In addition, the nucleation of CH4 bubbles was found to be more instantaneous than that of CO2 or C3H8 bubbles. Numerically, a robust kinetic reaction model in the commercial CMG-STARS module was utilized to simulate the gas exsolution and liberation processes of the CCE and DFP tests. The respective reaction frequency factors for gas exsolution (rffe) and liberation (rffl) were obtained in the numerical simulations. Higher values of rffe were found for the tests in the porous media in comparison with those in the bulk phases, suggesting that the presence of the porous media facilitated the gas exsolution. The magnitudes of rffe for the three different heavy oil–solvent systems followed the order of CO2 > CH4 > C3H8 in the bulk phases and CH4 > CO2 > C3H8 in the porous media. Hence, CO2 was exsolved from the heavy oil most readily in the bulk phases, whereas CH4 was exsolved from the heavy oil most easily in the porous media. Among the three solvents, CH4 was also found most difficult to be liberated from the heavy oil in the DFP test with the lowest rffl of 0.00019 min−1. This study indicates that foamy-oil evolution processes in the heavy oil reservoirs are rather different from those observed from the bulk-phase tests, such as the PVT tests. Full article
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11 pages, 574 KiB  
Article
Prediction of Failure to Progress after Labor Induction: A Multivariable Model Using Pelvic Ultrasound and Clinical Data
by Blanca Novillo-Del Álamo, Alicia Martínez-Varea, Elena Satorres-Pérez, Mar Nieto-Tous, Fernando Modrego-Pardo, Carmen Padilla-Prieto, María Victoria García-Florenciano, Silvia Bello-Martínez de Velasco and José Morales-Roselló
J. Pers. Med. 2024, 14(5), 502; https://doi.org/10.3390/jpm14050502 (registering DOI) - 9 May 2024
Abstract
Objective: Labor induction is one of the leading causes of obstetric admission. This study aimed to create a simple model for predicting failure to progress after labor induction using pelvic ultrasound and clinical data. Material and Methods: A group of 387 singleton pregnant [...] Read more.
Objective: Labor induction is one of the leading causes of obstetric admission. This study aimed to create a simple model for predicting failure to progress after labor induction using pelvic ultrasound and clinical data. Material and Methods: A group of 387 singleton pregnant women at term with unruptured amniotic membranes admitted for labor induction were included in an observational prospective study. Clinical and ultrasonographic variables were collected at admission prior to the onset of contractions, and labor data were collected after delivery. Multivariable logistic regression analysis was applied to create several models to predict cesarean section due to failure to progress. Afterward, the most accurate and reproducible model was selected according to the lowest Akaike Information Criteria (AIC) with a high area under the curve (AUC). Results: Plausible parameters for explaining failure to progress were initially obtained from univariable analysis. With them, several multivariable analyses were evaluated. Those parameters with the highest reproducibility included maternal age (p < 0.05), parity (p < 0.0001), fetal gender (p < 0.05), EFW centile (p < 0.01), cervical length (p < 0.01), and posterior occiput position (p < 0.001), but the angle of descent was disregarded. This model obtained an AIC of 318.3 and an AUC of 0.81 (95% CI 0.76–0.86, p < 0.0001) with detection rates of 24% and 37% for FPRs of 5% and 10%. Conclusions: A simplified clinical and sonographic model may guide the management of pregnancies undergoing labor induction, favoring individualized patient management. Full article
(This article belongs to the Section Personalized Critical Care)
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18 pages, 5484 KiB  
Article
Transmission Line Fault Classification Using Conformer Convolution-Augmented Transformer Model
by Meng-Yun Lee, Yu-Shan Huang, Chia-Jui Chang, Jia-Yu Yang, Chih-Wen Liu, Tzu-Chiao Lin and Yen-Bor Lin
Appl. Sci. 2024, 14(10), 4031; https://doi.org/10.3390/app14104031 (registering DOI) - 9 May 2024
Abstract
Ensuring a consistently reliable power supply is paramount in power systems. Researchers are engaged in the pursuit of categorizing transmission line failures to design countermeasures for mitigating the associated financial losses. Our study employs a machine learning-based methodology, specifically the Conformer Convolution-Augmented Transformer [...] Read more.
Ensuring a consistently reliable power supply is paramount in power systems. Researchers are engaged in the pursuit of categorizing transmission line failures to design countermeasures for mitigating the associated financial losses. Our study employs a machine learning-based methodology, specifically the Conformer Convolution-Augmented Transformer model, to classify transmission line fault types. This model processes time series input data directly, eliminating the need for expert feature extraction. The training and validation datasets are generated through simulations conducted on a two-terminal transmission line, while testing is conducted on historical data consisting of 108 events that occurred in the Taiwan power system. Due to the limited availability of historical data, they are utilized solely for inference purposes. Our simulations are meticulously designed to encompass potential faults based on an analysis of historical data. A significant aspect of our investigation focuses on the impact of the sampling rate on input data, establishing that a rate of four samples per cycle is sufficient. This suggests that, for our specific classification tasks, relying on lower frequency data might be adequate, thereby challenging the conventional emphasis on high-frequency analysis. Eventually, our methodology achieves a validation accuracy of 100%, although the testing accuracy is lower at 88.88%. The discrepancy in testing accuracy can be attributed to the limited information and the small number of historical events, which pose challenges in bridging the gap between simulated data and real-world measurements. Furthermore, we benchmarked our method against the ELM model proposed in 2023, demonstrating significant improvements in testing accuracy. Full article
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17 pages, 799 KiB  
Review
Recent Advances in IRAK1: Pharmacological and Therapeutic Aspects
by Kyeong Min Kim, Na-Hee Hwang, Ja-Shil Hyun and Dongyun Shin
Molecules 2024, 29(10), 2226; https://doi.org/10.3390/molecules29102226 (registering DOI) - 9 May 2024
Abstract
Interleukin receptor-associated kinase (IRAK) proteins are pivotal in interleukin-1 and Toll-like receptor-mediated signaling pathways. They play essential roles in innate immunity and inflammation. This review analyzes and discusses the physiological functions of IRAK1 and its associated diseases. IRAK1 is involved in a wide [...] Read more.
Interleukin receptor-associated kinase (IRAK) proteins are pivotal in interleukin-1 and Toll-like receptor-mediated signaling pathways. They play essential roles in innate immunity and inflammation. This review analyzes and discusses the physiological functions of IRAK1 and its associated diseases. IRAK1 is involved in a wide range of diseases such as dry eye, which highlights its potential as a therapeutic target under various conditions. Various IRAK1 inhibitors, including Pacritinib and Rosoxacin, show therapeutic potential against malignancies and inflammatory diseases. The covalent IRAK1 inhibitor JH-X-119-01 shows promise in B-cell lymphomas, emphasizing the significance of covalent bonds in its activity. Additionally, the emergence of selective IRAK1 degraders, such as JNJ-101, provides a novel strategy by targeting the scaffolding function of IRAK1. Thus, the evolving landscape of IRAK1-targeted approaches provides promising avenues for increasingly safe and effective therapeutic interventions for various diseases. Full article
(This article belongs to the Special Issue Anti-inflammatory Molecules)
21 pages, 10222 KiB  
Article
Design, In Vitro Evaluation and In Vivo Biocompatibility of Additive Manufacturing Three-Dimensional Printing of β beta-Tricalcium Phosphate Scaffolds for Bone Regeneration
by José Javier Llorente, Luis Junquera, Lorena Gallego, Marcos Pérez-Basterrechea, Luis Ignacio Suárez and Santiago Llorente
Biomedicines 2024, 12(5), 1049; https://doi.org/10.3390/biomedicines12051049 (registering DOI) - 9 May 2024
Abstract
The reconstruction of bone deficiencies remains a challenge due to the limitations of autologous bone grafting. The objective of this study is to evaluate the bone regeneration efficacy of additive manufacturing of tricalcium phosphate (TCP) implants using lithography-based ceramic manufacturing (LCM). LCM uses [...] Read more.
The reconstruction of bone deficiencies remains a challenge due to the limitations of autologous bone grafting. The objective of this study is to evaluate the bone regeneration efficacy of additive manufacturing of tricalcium phosphate (TCP) implants using lithography-based ceramic manufacturing (LCM). LCM uses LithaBone TCP 300 slurry for 3D printing, producing cylindrical scaffolds. Four models of internal scaffold geometry were developed and compared. The in vitro studies included cell culture, differentiation, seeding, morphological studies and detection of early osteogenesis. The in vivo studies involved 42 Wistar rats divided into four groups (control, membrane, scaffold (TCP) and membrane with TCP). In each animal, unilateral right mandibular defects with a total thickness of 5 mm were surgically performed. The animals were sacrificed 3 and 6 months after surgery. Bone neoformation was evaluated by conventional histology, radiology, and micro-CT. Model A (spheres with intersecting and aligned arrays) showed higher penetration and interconnection. Histological and radiological analysis by micro-CT revealed increased bone formation in the grafted groups, especially when combined with a membrane. Our innovative 3D printing technology, combined with precise scaffold design and efficient cleaning, shows potential for bone regeneration. However, further refinement of the technique and long-term clinical studies are crucial to establish the safety and efficacy of these advanced 3D printed scaffolds in human patients. Full article
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25 pages, 5609 KiB  
Article
Optimal Wind Farm Siting Using a Fuzzy Analytic Hierarchy Process: Evaluating the Island of Andros, Greece
by Dimitris Ioannidis and Dimitra G. Vagiona
Sustainability 2024, 16(10), 3971; https://doi.org/10.3390/su16103971 (registering DOI) - 9 May 2024
Abstract
In recent decades, fuzzy logic and fuzzy multi-criteria decision-making systems have been applied in several fields. This paper aims to determine the optimal wind farm siting solution in a fuzzy environment. Therefore, the main research question of the present paper is whether and [...] Read more.
In recent decades, fuzzy logic and fuzzy multi-criteria decision-making systems have been applied in several fields. This paper aims to determine the optimal wind farm siting solution in a fuzzy environment. Therefore, the main research question of the present paper is whether and to what extent the uncertainty in the researcher’s judgments affects the ranking of wind farm siting solutions. The fuzzy analytical hierarchy method is applied to an existing case study of wind farm siting on the island of Andros, examining the stability of the final priorities of the alternatives under a regime of gradual increases in ambiguity, as well as whether the introduced ambiguity in the model corresponds to any uncertainty the researcher has during the process of scoring the criteria and alternatives. Five assessment criteria (wind potential, ground slope, distance from road network, distance from high-voltage network, and social acceptance of local population) and eight eligible suitable alternatives (A1–A8) for wind farm siting are considered in the computations. The methodology includes the fuzzification of initial decision-maker judgments, the calculation of fuzzy intermediate priorities (weights), the defuzzification of fuzzy intermediate priorities (weights), and the synthesis of intermediate priorities into final priorities of alternatives, according to the procedures of the crisp AHP (CAHP). Under the assumptions of the initial case study, the results show that the final priorities are quite robust when faced with increased ambiguity. In almost all the examined cases, the alternative initially chosen as the best, A1, is dominant, followed by A3. In addition, in all cases, social acceptance favors alternative A1, and wind velocity favors alternative A8. Therefore, fuzzy multi-criteria methods can be applied to determine an optimal wind farm siting solution when criteria with qualitative characteristics are used and the manifestation of preferences involves strong elements of subjectivity. Full article
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33 pages, 2985 KiB  
Article
Assessing Lidar Ratio Impact on CALIPSO Retrievals Utilized for the Estimation of Aerosol SW Radiative Effects across North Africa, the Middle East, and Europe
by Anna Moustaka, Marios-Bruno Korras-Carraca, Kyriakoula Papachristopoulou, Michael Stamatis, Ilias Fountoulakis, Stelios Kazadzis, Emmanouil Proestakis, Vassilis Amiridis, Kleareti Tourpali, Thanasis Georgiou, Stavros Solomos, Christos Spyrou, Christos Zerefos and Antonis Gkikas
Remote Sens. 2024, 16(10), 1689; https://doi.org/10.3390/rs16101689 (registering DOI) - 9 May 2024
Abstract
North Africa, the Middle East, and Europe (NAMEE domain) host a variety of suspended particles characterized by different optical and microphysical properties. In the current study, we investigate the importance of the lidar ratio (LR) on Cloud-Aerosol Lidar with Orthogonal Polarization–Cloud-Aerosol Lidar and [...] Read more.
North Africa, the Middle East, and Europe (NAMEE domain) host a variety of suspended particles characterized by different optical and microphysical properties. In the current study, we investigate the importance of the lidar ratio (LR) on Cloud-Aerosol Lidar with Orthogonal Polarization–Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observations (CALIOP-CALIPSO) aerosol retrievals towards assessing aerosols’ impact on the Earth-atmosphere radiation budget. A holistic approach has been adopted involving collocated Aerosol Robotic Network (AERONET) observations, Radiative Transfer Model (RTM) simulations, as well as reference radiation measurements acquired using spaceborne (Clouds and the Earth’s Radiant Energy System-CERES) and ground-based (Baseline Surface Radiation Network-BSRN) instruments. We are assessing the clear-sky shortwave (SW) direct radiative effects (DREs) on 550 atmospheric scenes, identified within the 2007–2020 period, in which the primary tropospheric aerosol species (dust, marine, polluted continental/smoke, elevated smoke, and clean continental) are probed using CALIPSO. RTM runs have been performed relying on CALIOP retrievals in which the default and the DeLiAn (Depolarization ratio, Lidar ratio, and Ångström exponent)-based aerosol-speciated LRs are considered. The simulated fields from both configurations are compared against those produced when AERONET AODs are applied. Overall, the DeLiAn LRs leads to better results mainly when mineral particles are either solely recorded or coexist with other aerosol species (e.g., sea-salt). In quantitative terms, the errors in DREs are reduced by ~26–27% at the surface (from 5.3 to 3.9 W/m2) and within the atmosphere (from −3.3 to −2.4 W/m2). The improvements become more significant (reaching up to ~35%) for moderate-to-high aerosol loads (AOD ≥ 0.2). Full article
41 pages, 762 KiB  
Review
Anomaly Detection in Blockchain Networks Using Unsupervised Learning: A Survey
by Christos Cholevas, Eftychia Angeli, Zacharoula Sereti, Emmanouil Mavrikos and George E. Tsekouras
Algorithms 2024, 17(5), 201; https://doi.org/10.3390/a17050201 (registering DOI) - 9 May 2024
Abstract
In decentralized systems, the quest for heightened security and integrity within blockchain networks becomes an issue. This survey investigates anomaly detection techniques in blockchain ecosystems through the lens of unsupervised learning, delving into the intricacies and going through the complex tapestry of abnormal [...] Read more.
In decentralized systems, the quest for heightened security and integrity within blockchain networks becomes an issue. This survey investigates anomaly detection techniques in blockchain ecosystems through the lens of unsupervised learning, delving into the intricacies and going through the complex tapestry of abnormal behaviors by examining avant-garde algorithms to discern deviations from normal patterns. By seamlessly blending technological acumen with a discerning gaze, this survey offers a perspective on the symbiotic relationship between unsupervised learning and anomaly detection by reviewing this problem with a categorization of algorithms that are applied to a variety of problems in this field. We propose that the use of unsupervised algorithms in blockchain anomaly detection should be viewed not only as an implementation procedure but also as an integration procedure, where the merits of these algorithms can effectively be combined in ways determined by the problem at hand. In that sense, the main contribution of this paper is a thorough study of the interplay between various unsupervised learning algorithms and how this can be used in facing malicious activities and behaviors within public and private blockchain networks. The result is the definition of three categories, the characteristics of which are recognized in terms of the way the respective integration takes place. When implementing unsupervised learning, the structure of the data plays a pivotal role. Therefore, this paper also provides an in-depth presentation of the data structures commonly used in unsupervised learning-based blockchain anomaly detection. The above analysis is encircled by a presentation of the typical anomalies that have occurred so far along with a description of the general machine learning frameworks developed to deal with them. Finally, the paper spotlights challenges and directions that can serve as a comprehensive compendium for future research efforts. Full article
(This article belongs to the Special Issue Deep Learning for Anomaly Detection)
14 pages, 865 KiB  
Article
Investigating the Effects of Center of Gravity (CoG) Shift Due to a Simulated Exploration Extravehicular Mobility Unit (xEMU) Suit on Balance
by Roni A. Romero Melendez and Lara A. Thompson
Appl. Sci. 2024, 14(10), 4032; https://doi.org/10.3390/app14104032 (registering DOI) - 9 May 2024
Abstract
Maintaining balance is critical to minimizing astronauts’ risk of falling and reducing injury or suit damage. Previous studies involving spacesuits have not examined the effects of the superior shift of the center of gravity (CoG) on astronauts’ ability to balance. Here, the purpose [...] Read more.
Maintaining balance is critical to minimizing astronauts’ risk of falling and reducing injury or suit damage. Previous studies involving spacesuits have not examined the effects of the superior shift of the center of gravity (CoG) on astronauts’ ability to balance. Here, the purpose of our study was to investigate the effects of CoG shift due to a simulated Extravehicular Mobility Unit (xEMU) on balance. Seventeen participants’ standing balance was examined for three test configurations: unsuited, weighted with an Extravehicular Mobility Unit (xEMU) vest, and xEMU hard upper body torso (HUT). Using a Tekscan forceplate walkway, the CoG locations were determined. Balance assessments were performed to determine the limits of stability and standing balance performance during wide or tandem stances with eyes open/closed. The center of pressure (CoP) time series was examined in terms of displacement, velocity, and frequency measures. During the eyes-open wide stance, the xEMU vest significantly increased the mediolateral balance parameters, while the HUT significantly increased the total displacement (TOTEX), mean velocity (MVELO), and mean frequency (MFREQ) of the CoP. In the eyes-closed wide stance, the HUT significantly increased these parameters. In the eyes-closed tandem stance, the xEMU vest significantly decreased the parameters. The xEMU vest significantly reduced the TOTEX, MVELO, and MFREQ (improved standing balance), while the HUT decreased standing balance ability, seen with significant increases in said parameters. By quantifying CoG’s effect on balance, our results form the basis for future balance and posture studies of xEMU spacesuits. Full article
(This article belongs to the Section Biomedical Engineering)
10 pages, 3717 KiB  
Article
A Color-Detectable Vitamin C Controlled-Release System Fabricated Using Electrospinning
by Min Jae Shin
Polymers 2024, 16(10), 1347; https://doi.org/10.3390/polym16101347 (registering DOI) - 9 May 2024
Abstract
This study develops a vitamin C controlled-release system, trackable via color changes as a function of vitamin C release. The system is composed of coaxial microfibers prepared via coaxial electrospinning, with a core of poly(ethylene oxide) (PEO) incorporating vitamin C, and a shell [...] Read more.
This study develops a vitamin C controlled-release system, trackable via color changes as a function of vitamin C release. The system is composed of coaxial microfibers prepared via coaxial electrospinning, with a core of poly(ethylene oxide) (PEO) incorporating vitamin C, and a shell composed of polycaprolactone (PCL) containing polydiacetylene (PDA) as the color-changing material. The shell thickness is controlled by adjusting the amount of PCL ejected during electrospinning, allowing regulation of the release rate of vitamin C. When vitamin C added to PEO penetrates the PCL layer, the color of PDA changes from blue to red, indicating a color change. The results of this study can be applied to devices that require immediate detection of vitamin C release levels. Full article
(This article belongs to the Section Smart and Functional Polymers)
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18 pages, 13809 KiB  
Article
Machine Learning Prediction and Evaluation for Structural Damage Comfort of Suspension Footbridge
by Shaojie Zhao, Xing Tang and Yongjun Du
Buildings 2024, 14(5), 1344; https://doi.org/10.3390/buildings14051344 (registering DOI) - 9 May 2024
Abstract
To investigate the impact of structural damages on the comfort level of suspension footbridges under human-induced vibrations, this study addresses the limitations of traditional manual testing, which often entails significant manpower and material resources. The aim is to achieve rapid estimation and health [...] Read more.
To investigate the impact of structural damages on the comfort level of suspension footbridges under human-induced vibrations, this study addresses the limitations of traditional manual testing, which often entails significant manpower and material resources. The aim is to achieve rapid estimation and health monitoring of comfort levels during bridge operation. To accomplish this, the study combines finite-element simulation results to establish a data-driven library and introduces three distinct machine learning algorithms. Through comparative analysis, a machine learning-based method is proposed for quick evaluation of bridge comfort levels. Focusing on the Yangjiadong Suspension Bridge, the study evaluates and researches the comfort level of the structure under the influence of human-induced vibrations. The findings revealed a relatively low base frequency and high flexibility. Additionally, when considering the mass of individuals, peak acceleration decreased. The predictive performance of the Artificial Neural Network (ANN) model was found to be superior when accounting for multi-parameter damages, yielding root mean square error (RMSE), mean absolute percentage error (MAPE), and R-squared (R2) values of 0.03, 0.02, and 0.98, respectively. Moreover, the error ratio of the generalization performance analysis was below 5%. Furthermore, the study identified a damage coefficient of 0.13 for the bridge’s main cable, hanger, and steel longitudinal beam. Under a crowd density of 0.5 people per square meter, the predicted peak acceleration was 1.098 m/s2, with a model error of less than 10% compared to the observed value of 1.004 m/s2. These results underscore the model’s effectiveness in swiftly evaluating bridge comfort levels, thereby offering valuable insights for the health monitoring of bridge comfort levels. Full article
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23 pages, 4486 KiB  
Article
Estimation of the Shear Viscosity of Mixed-Polymer Materials for Screw Extrusion-Based Recycling Process Modeling
by Christian Kneidinger, Emil Wagner, Manuel Längauer and Gernot Zitzenbacher
Polymers 2024, 16(10), 1339; https://doi.org/10.3390/polym16101339 (registering DOI) - 9 May 2024
Abstract
The scope of this work is the development of a method to estimate the temperature and shear rate-dependent viscosity of mixtures composed of two polymers. The viscosity curve of polymer mixtures is crucial for the modeling and optimization of extrusion-based recycling, which is [...] Read more.
The scope of this work is the development of a method to estimate the temperature and shear rate-dependent viscosity of mixtures composed of two polymers. The viscosity curve of polymer mixtures is crucial for the modeling and optimization of extrusion-based recycling, which is the most efficient way to recycle polymeric materials. The modeling and simulation of screw extruders requires detailed knowledge of the properties of the processed material, such as the thermodynamic properties, the density, and the rheological behavior. These properties are widely known for pure materials; however, the incorporation of impurities, like other polymers in recycled materials, alters the properties. In this work, miscible, immiscible, and compatibilized immiscible polymer mixtures are considered. A new method based on shear stress is proposed and compared to the shear rate-based method. Several mixing rules are evaluated for their accuracy in predicting mixture viscosity. The developed methods allow the prediction of the viscosity of a compatibilized immiscible mixture with deviations below 5% and that of miscible polymer mixtures with deviations below 3.5%. Full article
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17 pages, 1284 KiB  
Article
In Situ Assessment of Uplink Duty Cycles for 4G and 5G Wireless Communications
by Günter Vermeeren, Leen Verloock, Sam Aerts, Luc Martens and Wout Joseph
Sensors 2024, 24(10), 3012; https://doi.org/10.3390/s24103012 (registering DOI) - 9 May 2024
Abstract
In this presented study, we measured in situ the uplink duty cycles of a smartphone for 5G NR and 4G LTE for a total of six use cases covering voice, video, and data applications. The duty cycles were assessed at ten positions near [...] Read more.
In this presented study, we measured in situ the uplink duty cycles of a smartphone for 5G NR and 4G LTE for a total of six use cases covering voice, video, and data applications. The duty cycles were assessed at ten positions near a 4G and 5G base-station site in Belgium. For Twitch, VoLTE, and WhatsApp, the duty cycles ranged between 4% and 22% in time, both for 4G and 5G. For 5G NR, these duty cycles resulted in a higher UL-allotted time due to time division duplexing at the 3.7 GHz frequency band. Ping showed median duty cycles of 2% for 5G NR and 50% for 4G LTE. FTP upload and iPerf resulted in duty cycles close to 100%. Full article
(This article belongs to the Section Communications)

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