This study aimed to explore the sustainable aesthetic and functional dimensions of environmental waste in the context of interior design applications by students. Employing both descriptive and applied methodologies, a series of artistic works derived from environmental waste, specifically metal and wood, were conceptualized and executed by art education students. These works, totaling 11 artistic models in interior design, underwent chemical treatment as part of the process. An evaluation card, assessed by arbitrators, was utilized to gauge the standards of aesthetic and functional sustainability inherent in the artworks. Technical data were collected and subsequently analyzed using SPSS software, which facilitated the calculation of arithmetic averages, standard deviations, and t-tests to ascertain the extent to which sustainability standards were met within the aesthetic and functional dimensions of the works. The study findings indicated that the average response scores for the aesthetic and functional dimensions, pertaining to the achievement of sustainability for wood and metal waste, were notably high. This underscores the potential of producing artful works suitable for interior design applications within the spaces of the College of Education. With an average score of 3.984, students exhibited positive engagement with the aesthetic and functional aspects of their artistic products, indicative of their considerable ethical significance. This augurs well for the feasibility of attaining sustainability through the recycling of wood and metal waste. Furthermore, this research underscores the necessity of integrating aesthetic, environmental, and social values in achieving sustainable aesthetic and functional environmental values within the interior design curriculum. This integration demands a comprehensive understanding of user expectations, technological advancements, and the cultural background, customs, and traditions of both users and society at large. Full article

Introduction: Bronchiectasis is a chronic progressive respiratory disease characterized by permanent dilatation of the bronchi. It is a complex condition with numerous different etiologies, co-morbidities, and a heterogeneous presentation. As we know, there is a lack of studies that describe the differences and compare the characteristics between focal and multifocal bronchiectasis. The aim of this study is to identify differences in clinical characteristics presentation, severity or distribution in focal and multifocal bronchiectasis, and prognostic implications. Methods: 126 patients with computed tomography (CT)-verified bronchiectasis were enrolled. Baseline characteristics that included age, sex, smoking history, and respiratory symptoms were recorded, with special attention paid to hemoptysis appearance, body mass index, and comorbidities. The type of bronchiectasis determined by CT scan and the modified Reiff scores indicating radiological severity were recorded. Patients were divided in two groups (I is focal and II is multifocal). Results: There were no statistically significant differences in age, smoking status, comorbidity, and BMI between the two groups. Multifocality was associated with a significantly higher proportion of females (p = 0.014), the rate of hemoptysis (p = 0.023), and the number of hospitalizations, but not of exacerbations and prevalence of immunodeficiency (p = 0.049). Significantly, a high number of subjects with multifocality had bronchiectasis of moderate severity, and post-infective and asthma-associated phenotypes were the dominant in this group. Unexpectedly, the cystic and varicose radiological phenotype (which need more time to develop) were more dominant in the focal group. The cylindrical phenotype was equally observed in the multifocal and focal groups. Conclusions: Our study suggests that multifocality is not related to age, number of exacerbations, or radiological phenotype, but it seems to be associated with the clinical post-infective phenotype, immunodeficiency, frequent hospitalizations, and severity. Thus, the presence of multifocal bronchiectasis may act as a biomarker of severity and poor outcomes in these patients. Full article

This study aimed to quantify and compare ladder use ability and behavior in younger and older men and women from three ladder use behavior experiments. The experimental tasks comprised (1) changing a lightbulb on a household stepladder under two cognitive demands (single and dual task), (2) clearing a simulated roof gutter on a straight ladder and (3) querying ladder choice in different exigency scenarios. Ladder use ability and behavior data were captured from recorded time, performance, motion capture and user choice data. In addition, this study surveyed ladder use frequency and habitual behaviors. The experimental findings indicate that older adults require more time to complete ladder tasks; younger adults display riskier ladder use behaviors; men and women display similar ladder use ability; and men are more willing to climb riskier ladders. The survey found older adults to report more frequent ladder use than younger adults, and men use straight ladders more frequently than women. These results suggest that the reported higher ladder fall rates experienced by older adults and men are linked to increased ladder use exposure and riskier ladder choice. This knowledge can help guide population-specific interventions to reduce ladder falls in both young and older people. Full article

Amidst today’s globalized economy, embedding sustainable practices into corporate governance frameworks has become important. This era underscores a heightened focus on CSR and sustainability, drawing considerable scholarly and practical interest to the nexus of corporate governance, sustainability initiatives, and corporate labour rights (CLRs). Hence, this study investigates the relationship between corporate sustainability governance initiatives (CSGIs) and CLR practices in India. Despite regulatory efforts, issues such as modern slavery and low wages persist in the country. Drawing on legitimacy, agency, stakeholder, and resource dependency theories, this study examines how CSGIs influence CLR practices. Data from 1212 observations of top Indian listed companies between 2010 and 2021 indicate positive correlations between CSGI dimensions and CLR practices across industries and board CSR orientations. This research underscores the importance of CSGIs in promoting sustainable corporate practices and improving CLRs in emerging economies. It also aligns with the United Nations sustainable development goals (SDGs), particularly SDG 3, SDG 5, SDG 8, and SDG 10. By combining theoretical rigour with practical relevance, this study provides insights for businesses, policymakers, workers, investors, and CSR scholars, contributing to efforts to enhance CLRs in India and beyond. For instance, the study offers actionable guidance for businesses and policymakers aiming to improve CLR practices. It highlights the positive correlation between specific attributes of CSGIs and CLR practices, providing insights for corporate decision-making and emphasizing the importance of aligning operations with the UN’s sustainable development goals. The findings serve as critical decision-making tools for investors concerned with corporate sustainability governance and CLRs to identify ethically responsible companies and mitigate investment risks. Full article

Studies on bioaerosol bacterial biodiversity have relevance in both ecological and health contexts, and molecular methods, such as 16S rRNA gene-based barcoded sequencing, provide efficient tools for the analysis of airborne bacterial communities. Standardized methods for sampling and analysis of bioaerosol DNA are lacking, thus hampering the comparison of results from studies implementing different devices and procedures. Three samplers that use gelatin filtration, swirling aerosol collection, and condensation growth tubes for collecting bioaerosol at an aeration tank of a wastewater treatment plant in Trieste (Italy) were used to determine the bacterial biodiversity. Wastewater samples were collected directly from the untreated sewage to obtain a true representation of the microbiological community present in the plant. Different samplers and collection media provide an indication of the different grades of biodiversity, with condensation growth tubes and DNA/RNA shield^TM capturing the richer bacterial genera. Overall, in terms of relative abundance, the air samples have a lower number of bacterial genera (64 OTUs) than the wastewater ones (75 OTUs). Using the metabarcoding approach to aerosol samples, we provide the first preliminary step toward the understanding of a significant diversity between different air sampling systems, enabling the scientific community to orient research towards the most informative sampling strategy. Full article

Cyclodextrins (CDs) were used as green char promoters in the formulation of organophosphorus flame retardants (OPFRs) for polymeric materials, and they could reduce the amount of usage of OPFRs and their release into the environment by forming [host–guest] inclusion complexes with them. Here, we report a systematic study on the inclusion complexes of natural CDs (α-, β-, and γ-CD) with a representative OPFR of DOPO using computational methods of molecular docking, molecular dynamics (MD) simulations, and quantum mechanical (QM) calculations. The binding modes and energetics of host–guest inclusion complexes were analyzed in details. α-CD was not able to form a complete inclusion complex with DOPO, and the center of mass distance host–guest distance amounted to 4–5 Å. β-CD and γ-CD allowed for a deep insertion of DOPO into their hydrophobic cavities, and DOPO was able to frequently change its orientation within the γ-CD cavity. The energy decomposition analysis based on the dispersion-corrected density functional theory (sobEDAw) indicated that electrostatic, orbital, and dispersion contributions favored host–guest complexation, while the exchange–repulsion term showed the opposite. This work provides an in-depth understanding of using CD inclusion complexes in OPFRs formulations. Full article

Clarifying the optimal combination of N fertilizer application rate and application method can maximize the yield of drip-irrigated sugar beet in arid areas, which is of great significance for reducing farmland N pollution and achieving sustainable agricultural development. In this three-year field experiment in Xinjiang, China, the effects of three N application rates [75 kg ha⁻¹ (N1), 150 kg ha⁻¹ (N2), and 225 kg ha⁻¹ (N3)] and three N application methods [the proportion of N applied at canopy rapid growth stage, taproot expansion stage, and sugar accumulation stage were (M₁) 100%: 0%: 0%, (M₂) 70%: 30%: 0%, and (M₃) 50%: 30%: 20%] on the dry matter accumulation (DMA) and distribution, leaf senescence, yield, and agronomic N use efficiency (aNUE) of drip-irrigated sugar beet were explored. The results showed that N application (N1, N2, and N3 treatments) increased the shoot DMA by 27.7% (three-year average), 52.6%, and 83.1%, and the taproot DMA by 28.3%, 43.2%, and 61.6%, respectively (p < 0.05), compared with CK (no N supply) treatment. The N application methods M₂ and M₃ increased the shoot DMA by 5.6% (three-year average) and 1.0% (p > 0.05), respectively, and the taproot DMA by 7.2% and 3.6% (p < 0.05), respectively, compared with M₁. In addition, M₂ could delay the end of shoot and taproot growth (t_e) and the occurrence of maximum growth rate (t_m). In particular, the N3M₂ treatment increased the leaf area index (LAI) by 20.4–75.9% (p < 0.05) compared with other treatments by increasing the leaf area duration (LAD) and decreasing the leaf senescence rate (LSR). The taproot yield and sugar yield of N3M₂ treatment reached the maximum at harvest time, but there was no significant difference in taproot yield and sugar yield between N3M₂ treatment and N2M₂ treatment. The aNUE in N2M₂ treatment was the highest (p < 0.05), which was 1.29–7.85 times higher than that of other treatments. Therefore, reducing the N application rate from 225 kg·ha⁻¹ to 150 kg·ha⁻¹ and applying 70% and 30% of 150 kg N ha⁻¹ at the canopy rapid growth stage and the taproot expansion stage, respectively, could achieve the goal of increasing sugar beet yield and N use efficiency. This study will provide an important reference for the sustainable production of sugar beet under drip irrigation in Xinjiang, China. Full article

Background: Obesity is a critical public health concern with its prevalence growing at an alarming rate worldwide. The Western diet that typically includes high-fat or high-fructose components is one of the leading contributing factors of obesity. Recent findings demonstrate the essential role of BAT in regulating whole-body metabolism. However, the explicit mechanism through which BAT maintains homeostasis is still unknown. Methods: Six-week-old C57BL/6 male mice were fed either a low-fat diet (LFD) or a high-fat high-fructose diet (HFHFD) for 4, 12, and 20 weeks. Results: We observed a significant increase in BAT weight under HFHFD along with BAT whitening in a time-dependent manner. This was also accompanied by a significant decrease in UCP1 and PGC1α protein, as well as a significant increase in the Bax/Bcl-2 ratio as early as 12 weeks, indicating increased apoptosis under HFHFD. Interestingly, miRNA-103 expression that holds a seed sequence within the miRNA biogenesis machinery, Dicer, was significantly upregulated after 12 and 20 weeks of HFHFD. Dicer and another biogenesis regulator, TRBP2, exhibited significant upregulation at 4 weeks of HFHFD. Conversely, those gene expressions were significantly downregulated at 12 and 20 weeks of HFHFD, followed by a significant decrease in the protein level at 12 weeks. To confirm the mechanistic connection, miRNA-103 knockdown in vitro significantly upregulated Dicer and the TRBP2 gene. However, only Dicer exhibited a significant increase at the translational level. Conclusion: Overall, we conclude that HFHFD may elicit BAT dysfunction by inhibiting Dicer via miRNA-103. Full article

A primary management concern when CSR is used as a tool to build business excellence is whether it will result in a discernible influence on the competitiveness of firms. This concern is more pronounced in the hypercompetitive, customer-centric grocery industry. Despite the existing body of research, no study has combined CSR’s effects on competitiveness and business excellence into a model. The current study proposes and validates a data-driven conceptual model that aims to assess whether the integration of CSR into supermarkets’ (S/Ms’) business excellence practices can enhance their competitiveness. Relationships among the validated constructs were examined using structural equation modeling. Findings indicated that the competitiveness of supermarkets can be enhanced by incorporating CSR into the process of pursuing business excellence, but CSR may be deprioritized in favor of more pressing performance-related issues. Large S/M firms could mentor and advocate for smaller stores and encourage CSR integration through government initiatives. Full article

This manuscript describes the computational process to calculate an airplane path and display it in a 2D and 3D coordinate system on a computer screen. The airplane movement is calculated as a function of its dynamic’s conditions according to physical and logical theory. Here, the flight is divided into maneuvers and the aircraft conditions are defined as boundary conditions. Then the aircraft position is calculated using nested loops, which execute the calculation procedure at every step time (Δt). The calculation of the aircraft displacement is obtained as a function of the aircraft speed and heading angles. The simulator was created using the C++ programming language, and each part of the algorithm was compiled independently to reduce the source code, allow easy modification, and improve the programming efficiency. Aerial navigation involves very complex phenomena to be considered for an appropriate representation; moreover, in this manuscript, the influence of the mathematical approach to properly represent the aircraft flight is described in detail. The flight simulator was successfully tested by simulating some basic theoretical flights with different maneuvers, which include stationary position, running along the way, take off, and some movements in the airspace. The maximum aircraft speed tested was 120 km/h, the maximum maneuver time was 12 min, and the space for simulation was assumed to be without obstacles. Here, the geometrical description of path and speed is analyzed according to the symmetric and asymmetric results. Finally, an analysis was conducted to evaluate the approach of the numerical methods used; after that, it was possible to confirm that precision increased as the step time was reduced. According to this analysis, no more than 500 steps are required for a good approach in the calculation of the aircraft displacement. Full article

The sneaker industry is continuing to expand at a fast rate and will be worth over USD 120 billion in the next few years. This is, in part due to social media and online retailers building hype around releases of limited-edition sneakers, which are usually collaborations between well-known global icons and footwear companies. These limited-edition sneakers are typically released in low quantities using an online raffle system, meaning only a few people can get their hands on them. As expected, this causes their value to skyrocket and has created an extremely lucrative resale market for sneakers. This has given rise to numerous counterfeit sneakers flooding the resale market, resulting in online platforms having to hand-verify a sneaker’s authenticity, which is an important but time-consuming procedure that slows the selling and buying process. To speed up the authentication process, Support Vector Machines and a convolutional neural network were used to classify images of fake and real sneakers and then their accuracies were compared to see which performed better. The results showed that the CNNs performed much better at this task than the SVMs with some accuracies over 95%. Therefore, a CNN is well equipped to be a sneaker authenticator and will be of great benefit to the reselling industry. Full article

Clown doctors play a crucial role in enhancing the well-being of patients through the use of humor. However, little is known about how the use of humor by clown doctors changes in relation to the developmental age of patients. This research explores the interplay between the type of humor used by clown doctors, their experience (in terms of years of clowning and type of clowning), and the developmental age of the patients (children, adolescents, adults, elderly). Data for this cross-sectional study were collected through an online survey distributed to 210 Italian clown doctors (143 females, 67 males), aged between 18 and 75 years (M = 47.34, SD = 12.31), affiliated with different Clown Care Units. The survey included the Comic Styles Markers, questions on the patients’ developmental age, type of clowning (Auguste vs. Whiteface), and years of experience. The findings enhance our understanding on how clown doctors interact with patients of different developmental ages. The discussion draws connections to previous studies conducted on groups of clown doctors, providing a broader context for understanding the implications of humorous interactions in this unique healthcare domain. Full article

Cervical artery dissection (CeAD) is the primary cause of ischemic stroke in young adults. Monogenic heritable connective tissue diseases account for fewer than 5% of cases of CeAD. The remaining sporadic cases have known risk factors. The clinical, radiological, and histological characteristics of systemic vasculopathy and undifferentiated connective tissue dysplasia are present in up to 70% of individuals with sporadic CeAD. Genome-wide association studies identified CeAD-associated genetic variants in the non-coding genomic regions that may impact the gene transcription and RNA processing. However, global gene expression profile analysis has not yet been carried out for CeAD patients. We conducted bulk RNA sequencing and differential gene expression analysis to investigate the expression profile of protein-coding genes in the peripheral blood of 19 CeAD patients and 18 healthy volunteers. This was followed by functional annotation, heatmap clustering, reports on gene–disease associations and protein–protein interactions, as well as gene set enrichment analysis. We found potential correlations between CeAD and the dysregulation of genes linked to nucleolar stress, senescence-associated secretory phenotype, mitochondrial malfunction, and epithelial–mesenchymal plasticity. Full article

This study aims to evaluate the energy-saving potential of passive solar heating systems in diverse global climates and introduce a new indicator, the passive solar heating indicator (PSHI), to enhance the efficiency of building designs. By collecting climate data from 600 cities worldwide through a simulation model, the present study employs polynomial regression to analyze the impact of outdoor temperature and solar radiation intensity on building energy savings. It also uses K-means cluster analysis to scientifically categorize cities based on their energy-saving potential. The findings underscore the benefits of both direct and indirect solar heating strategies in different climates. Significantly, the PSHI shows superior predictive accuracy and applicability over traditional indices, such as the irradiation temperature difference ratio (ITR) and the irradiation degree hour ratio (C-IDHR), especially when outdoor temperatures are close to indoor design temperatures. Moreover, the application of a cluster analysis provides hierarchical guidance on passive heating designs globally, paving the way for more accurate and customized energy-efficient building strategies. Full article

The application potential of additive manufacturing nickel-based superalloys in aeroengines and gas turbines is extensive, and evaluating their mechanical properties is crucial for promoting the engineering application in load-bearing components. In this study, Hastelloy X alloy was prepared using the laser powder bed fusion process combined with solution heat treatment. The tensile and high cycle fatigue properties were experimentally investigated at room temperature as well as two typical elevated temperatures, 650 °C and 815 °C. It was found that, during elevated-temperature tensile deformation, the alloy exhibits significant serrated flow behavior, primarily observed during the initial stage of plastic deformation at 650 °C but occurring throughout the entire plastic deformation process at 815 °C. Notably, when deformation is small, sawtooth fluctuations are significantly higher at 815 °C compared to 650 °C. Irregular subsurface lack of fusion defects serve as primary sources for fatigue crack initiation in this alloy including both single-source and multi-source initiation mechanisms; moreover, oxidation on fracture surfaces is more prone to occur at elevated temperatures, particularly at 815 °C. Full article

In laser beam processing, the angle or offset between the auxiliary gas and the laser beam axis have been proved to be two new process optimization parameters for improving cutting speed and quality. However, a traditional electromechanical actuator cannot achieve high-speed and high-precision motion control with a compact structure. This paper proposes a magnetic levitation actuator which could realize the 5-DOF motion control of a lens using six groups of differential electromagnets. At first, the nonlinear characteristic of a magnetic driving force was analyzed by establishing an analytical model and finite element calculation. Then, the dynamic model of the magnetic levitation actuator was established using the Taylor series. And the mathematical relationship between the detected distance and five-degree-of-freedom was determined. Next, the centralized control system based on PID control was designed. Finally, a driving test was carried out to verify the five-degrees-of-freedom motion of the proposed electromagnetic levitation actuator. The results show it can achieve a stable levitation and precision positioning with a desired command motion. It also proves that the proposed magnetic levitation actuator has the potential application in an off-axis laser cutting machine tool. Full article

Optimization is a process of minimizing or maximizing a given objective function under specified constraints. In multi-objective optimization (MOO), multiple conflicting functions are optimized within defined criteria. Numerous MOO techniques have been developed utilizing various meta-heuristic methods such as Evolutionary Algorithms (EAs), Genetic Algorithms (GAs), and other biologically inspired processes. In a cooperative environment, a Pareto front is generated, and an MOO technique is applied to solve for the solution set. On other hand, Multi-Criteria Decision Making (MCDM) is often used to select a single best solution from a set of provided solution candidates. The Multi-Criteria Decision Making–Pareto Front (M-PF) optimizer combines both of these techniques to find a quality set of heuristic solutions. This paper provides an improved version of the M-PF optimizer, which is called the elite Multi-Criteria Decision Making–Pareto Front (eMPF) optimizer. The eMPF method uses an evolutionary algorithm for the meta-heuristic process and then generates a Pareto front and applies MCDM to the Pareto front to rank the solutions in the set. The main objective of the new optimizer is to exploit the Pareto front while also exploring the solution area. The performance of the developed method is tested against M-PF, Non-Dominated Sorting Genetic Algorithm-II (NSGA-II), and Non-Dominated Sorting Genetic Algorithm-III (NSGA-III). The test results demonstrate the performance of the new eMPF optimizer over M-PF, NSGA-II, and NSGA-III. eMPF was not only able to exploit the search domain but also was able to find better heuristic solutions for most of the test functions used. Full article

This paper presents a study on the efficient removal of nitrate nitrogen from wastewater using modified reed straw (MRS) and its subsequent resource utilization. The modification of the reed straw involved the introduction of branching quaternary amine groups to enhance its adsorption capacity for nitrate nitrogen. Experimental investigations were conducted to analyze the impact of packing height, flow rate, and initial solution concentration on the dynamic adsorption performance of the MRS. The results revealed that the maximum dynamic adsorption capacity of the MRS for nitrate nitrogen reached 14.76 mg/g. Furthermore, valuable nitrate nitrogen nutrient solution was successfully recovered through subsequent desorption experiments for resource recycling. Moreover, the application of the MRS led to notable enhancements in column height, fresh weight, chlorophyll content, and nitrogen content of the treated plants, indicating its efficacy in promoting plant growth. Overall, the findings demonstrate that MRS serves as a versatile adsorbent capable of efficient nitrate nitrogen removal and subsequent resource utilization. Full article

High-index contrast lithium niobate waveguides, fabricated by the High Vacuum Vapor-phase Proton Exchange (HiVac-VPE) technique, are very promising for increasing both the optical nonlinear and electro-optical efficiencies of photonic integrated devices. So as to play this role effectively, it is mandatory to know the crystallographic phase composition of waveguides and the position of protonated layers for appropriate tailoring and optimization based on the intended applications. In addition, the estimation of structural disorder and electro-optical properties of the waveguides are also of high interest. Benefiting from Raman spectroscopy, IR reflection, IR absorption, and UV-VIS absorption, the H_xLi_1−xNbO₃ phase compositions, as well as the structural disorder in waveguides, were determined. Based on experimental data on the shift of the fundamental absorption edge, we have quantitatively estimated the electro-optic coefficient r₁₃ in as-exchanged waveguides. The electro-optical properties of the waveguides have been found to be depending on the phase composition. The obtained results allow for reconsidering the proton exchange fabricating process of photonic nonlinear devices and electro-optic modulators based on high-index contrast channel waveguides on the LiNbO₃ platform. Full article

The majority of investigations on microbial communities from various environments are presently built on culture-independent methods. Many studies point to the pivotal, selective role of primers targeting hypervariable regions of 16S rRNA in the metabarcoding of bacteria, including cyanobacterial communities. The selectivity of primers designed to amplify targeted regions of the 16S rRNA gene, which has been highlighted by many authors, limited effective amplification. Moreover, the type and specificity of the studied material can also negatively influence the results of 16S metabarcoding. Most of the studies of cyanobacterial communities have been performed for planktonic microbial communities that are often represented by common, well-studied species. In this study, we present the results of 16S metabarcoding analysis using three primer pairs—two already well-known and a third designed in this study—that amplify divergent regions of the 16S rRNA gene (V3–V4, V4–V6, V6) for benthic, microbial mat-forming cyanobacteria communities. Such communities can be a source of toxigenic cyanobacterial taxa and should be monitored with adequate primers. The comparison of three primer pairs suggested that those designed within the present study describe the structure and composition of highly heterogeneous cyanobacterial mats’ communities better than the others. Full article

In this study, the balance control of a Brushless Direct Current Motor (BLDCM) driven Two-Rotor UAV (2R-UAV) was carried out. First, a MATLAB/Simulink model of the balance system of the 2R-UAV was built. Afterwards, classical and 2-DOF PID, and proposed Adaptive Fuzzy (AF) 2-DOF PID control structures were created on the STM32F4 microprocessor for both balance angle of the system and speed control of the BLDCMs. Classical and 2-DOF PID controller parameters were determined via Particle Swarm Optimization (PSO), a technique that is commonly used in control applications. For the balance control of the 2R-UAV, a Co-Simulation structure was created using the STM32F4 microprocessor and MATLAB/Simulink, and the performances of classical and 2-DOF PID, and AF 2-DOF PID controllers were examined comparatively. Upon examining the comparison results, it was found that the classical and 2-DOF PID, and AF 2-DOF PID stably controlled the balance of the 2R-UAV. The AF 2-DOF PID controller, proposed in this research, performed better than the classical and 2-DOF PID, especially under variable operating conditions. Full article

The understanding of the human genome has been greatly improved by the advent of next-generation sequencing technologies (NGS). Despite the undeniable advantages responsible for their widespread diffusion, these methods have some constraints, mainly related to short read length and the need for PCR amplification. As a consequence, long-read sequencers, called third-generation sequencing (TGS), have been developed, promising to overcome NGS. Starting from the first prototype, TGS has progressively ameliorated its chemistries by improving both read length and base-calling accuracy, as well as simultaneously reducing the costs/base. Based on these premises, TGS is showing its potential in many fields, including the analysis of difficult-to-sequence genomic regions, structural variations detection, RNA expression profiling, DNA methylation study, and metagenomic analyses. Protocol standardization and the development of easy-to-use pipelines for data analysis will enhance TGS use, also opening the way for their routine applications in diagnostic contexts. Full article