The COVID-19 has caused high morbidity and mortality in vulnerable people, such as those affected by chronic diseases, and case-management nurses (CMNs) are reference professionals for their health care and management. The objective of this study is to better understand the discourse, experiences, and feelings about the professional performance of CMNs during the pandemic. A qualitative study was conducted by conducting semi-structured interviews with CMNs (n = 31) from the province of Seville (Spain) and performing a narrative discourse analysis. The Atlas Ti 6.2 software program was used. Two categories were defined: 1. CMNs’ competencies (76 verbatim testimonies); and 2. Consequences of the COVID-19 pandemic (61 verbatim testimonies). This study was granted due permission by the Research Ethics Committee belonging to the University of Seville, under protocol code: 1139-N-22. The pandemic caused an increase in CMNs’ workload, and they had to assume their usual care tasks for vulnerable populations in addition to simultaneously prioritizing assistance in nursing homes. We can highlight CMNs’ adaptation to the pandemic situation and to these new requirements in the context of their significant social commitment to the advanced practice of the profession, a commitment that is closely related to leadership. We should also indicate that interpersonal relationships were improved, and that there was technological progress. Some CMNs mentioned an increase in their workload and reported experiencing burnout syndrome. We conclude that CMNs’ management of health care during the pandemic has been extraordinary, especially in regard to the most vulnerable populations of patients, including individuals with chronic diseases and institutionalized older adults, a fact that has been valued by the institutions and by society in general. Full article

Phosphogypsum (PG), a byproduct during the phosphoric acid production process, also known as the wet process, contains complex and diverse impurities, resulting in low utilization and considerable accumulation. This leads to a massive waste of land resources and a series of environmental pollution problems. Given the current urgent ecological and environmental situation, developing impurity removal processes with low energy consumption and high efficiency, exploring valuable resource recovery, preparing high value-added PG products, and broadening the comprehensive utilization ways of PG are significant strategies to promote the sustainable consumption of PG and sustainable development of the phosphorus chemical industry. This review comprehensively summarizes the advantages and disadvantages of existing PG impurity removal and utilization technologies and probes into the future development direction, which provides references and ideas for subsequent PG research. Full article

Immediate dentin sealing (IDS) is a method of improving the bond strength of indirect dental restorative materials to dentin and belongs to the biomimetic protocols of contemporary dentistry. The purpose of this systematic review was to evaluate the effect of IDS on the bond strength of resin-based CAD/CAM materials to dentin. PubMed and MEDLINE, Scopus, and the Web of Science were searched by two individual researchers, namely for studies that have been published in English between 1 January 2005 and 1 October 2022 in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. The inclusion criteria encompassed articles related to in vitro studies, measuring the bond strength through microtensile bond strength (μ-TBS), micro-shear bond strength (μ-SBS), tensile bond strength (TBS) or shear bond strength (SBS) tests after the use of the IDS technique. The included restorative materials comprised resin-based CAD/CAM materials bonded to dentin. A total of 1821 studies were identified, of which 7 met the inclusion criteria. A meta-analysis was not deemed appropriate due to the high level of diversity inthe publications and techniques. The use of IDS yielded higher bond strength outcomesin various experimental conditions and resin-based CAD/CAM materials. Overall, IDS in CAD/CAM restorations may contribute to better clinical outcomesand improved restoration longevity due to this property. Full article

In the current educational context, international institutions are being called to rethink education and declining new strategies that aim to teach sustainable behaviors. From this perspective, the present study aims to investigate the attitudes towards the sustainability of trainee teachers, verifying their connection with psycho-social variables, such as environmental identity and sense of community responsibility, and exploring their most common dispositions. In total, 126 Italian preservice teachers were involved in a mixed method study where they were asked to complete a questionnaire and participated in focus groups. The use of a combination of quantitative–qualitative methodologies has allowed us to obtain in-depth information about sustainability education. Quantitative data showed a virtuous circular relationship between environmental identity, attitudes towards education for sustainability, and the sense of community responsibility. Qualitative data showed the high frequency of two key action verbs, avoid and respect, but no specific reference to the educational field. After creating the target concept ‘to teach’, we observed a worrying absence of lemmas related to emotional dimension, and that the communication perspective is set on adults’ point of view, rather than on pupils. Limitations, strengths, and practical implications have been extensively discussed, especially in relation to the field of teacher education. Full article

Soccer is a laterally dominant sport owing to the repetitive nature of unilateral kicking. The relationship between functional and body composition asymmetries related to limb dominance in soccer players has yet to be established. When present, asymmetries can increase the risk of injury and low back pain. Our study investigated whether lateral dominance is associated with limb asymmetries in a comprehensive body composition assessment among varsity soccer players. Twenty-seven varsity soccer players (age 20.4 ± 1.7 years old; BMI 22.6 ± 4.6 kg/m²) participated in this study. Body composition was assessed through dual-energy X-ray absorptiometry scans. Results showed low lower limb asymmetry indices in both males (3.82%) and females (3.36%) compared to normal ranges. However, upper limb lean mass exhibited high asymmetry, surpassing thresholds in males (7.3%) and females (4.39%). Significant differences were found in total bone mass among males and total lean body mass among females. Male players exhibited higher asymmetry indices in both arm and trunk mass compared to females. Despite these asymmetries, no significant correlations were found between asymmetry indices and occurrences of lower limb injury or low back pain. The study suggests that while evaluating body composition for injury prevention in soccer shows potential, lateral dominance may be influenced by factors extending beyond sport-specific adaptations. Full article

With this follow-up paper, we continue developing a mathematical framework based on information geometry for representing physical objects. The long-term goal is to lay down informational foundations for physics, especially quantum physics. We assume that we can now model information sources as univariate normal probability distributions $\mathcal{N}(\mu ,{\sigma }_0)$, as before, but with a constant ${\sigma }_0$ not necessarily equal to 1. Then, we also relaxed the independence condition when modeling m sources of information. Now, we model m sources with a multivariate normal probability distribution ${\mathcal{N}}_m(\mu \mu \mu ,{\Sigma \Sigma \Sigma }_0)$ with a constant variance–covariance matrix ${\Sigma \Sigma \Sigma }_0$ not necessarily diagonal, i.e., with covariance values different to 0, which leads to the concept of modes rather than sources. Invoking Schrödinger’s equation, we can still break the information into m quantum harmonic oscillators, one for each mode, and with energy levels independent of the values of ${\sigma }_0$, altogether leading to the concept of “intrinsic”. Similarly, as in our previous work with the estimator’s variance, we found that the expectation of the quadratic Mahalanobis distance to the sample mean equals the energy levels of the quantum harmonic oscillator, being the minimum quadratic Mahalanobis distance at the minimum energy level of the oscillator and reaching the “intrinsic” Cramér–Rao lower bound at the lowest energy level. Also, we demonstrate that the global probability density function of the collective mode of a set of m quantum harmonic oscillators at the lowest energy level still equals the posterior probability distribution calculated using Bayes’ theorem from the sources of information for all data values, taking as a prior the Riemannian volume of the informative metric. While these new assumptions certainly add complexity to the mathematical framework, the results proven are invariant under transformations, leading to the concept of “intrinsic” information-theoretic models, which are essential for developing physics. Full article

Blends of polylactic acid (PLA) with amorphous polyhydroxyalkanoate (aPHA) are less brittle than neat PLA, thus enabling their use as biodegradable packaging. This work investigated the impact of recycling on the properties of neat PLA and PLA/aPHA blends with 90 and 75 wt. % PLA. After the materials were subjected to five heat histories in a single-screw extruder, the mechanical, rheological, and thermal properties were measured. All recycled compounds with 100% PLA and 75% PLA had similar decomposition behavior, whereas the decomposition temperatures for the blends with 90% PLA decreased with each additional heat cycle. The glass transition and melting temperatures were not impacted by reprocessing, but the crystallinity increased with more heat cycles. The complex viscosity of the reprocessed PLA and PLA/aPHA blends was much lower than for the neat PLA and increasing the number of heat cycles produced smaller reductions in the complex viscosity of 100% PLA and the blend with 90% PLA; no change in complex viscosity was observed for blends with 75% PLA exposed to 2 to 5 heat cycles. The tensile properties were not affected by reprocessing, whereas the impact strength for the 75% PLA blend decreased with reprocessing. These properties suggest that users will be able to incorporate scrap into the neat resin for thermoformed packaging. Full article

The main aim of this article is to derive certain continuity and boundedness properties of the coupled fractional Fourier transform on Schwartz-like spaces. We extend the domain of the coupled fractional Fourier transform to the space of tempered distributions and then study the mapping properties of pseudo-differential operators associated with the coupled fractional Fourier transform on a Schwartz-like space. We conclude the article by applying some of the results to obtain an analytical solution of a generalized heat equation. Full article

Hydrogen fuel cell tractors are emerging as a new power source for tractors. Currently, there is no mature energy management control method available. Existing methods mostly rely on engineers’ experience to determine the output power of the fuel cell and the power battery, resulting in relatively low energy utilization efficiency of the energy system. To address the aforementioned problems, a power optimization method for the energy system of hydrogen fuel cell wheel-driven electric tractor was proposed. A dynamic model of tractor ploughing conditions was established based on the system dynamics theory. From this, based on the equivalent hydrogen consumption theory, the charging and discharging of the power battery were equivalent to the fuel consumption of the hydrogen fuel cell, forming an equivalent hydrogen consumption model for the tractor. Using the state of charge (SOC) of the power battery as a constraint, and with the minimum equivalent hydrogen consumption as the objective function, an instantaneously optimized power allocation method based on load demand in the energy system is proposed by using a traversal algorithm. The optimization method was simulated and tested based on the MATLAB simulation platform, and the results showed under ploughing conditions, compared with the rule-based control strategy, the proposed energy system power optimization method optimized the power output of hydrogen fuel cells and power batteries, allowing the energy system to work in a high-efficiency range, reducing the equivalent hydrogen consumption of the tractor by 7.79%, and solving the energy system power distribution problem. Full article

Although both localized nuclear magnetic resonance spectroscopy (MRS) and non-localized nuclear magnetic resonance spectroscopy (NMR) generate the same information, i.e., spectra generated by various groups from the structure of metabolites, they are rarely employed in the same study or by the same research group. As our review reveals, these techniques have never been applied in the same study of methylmalonic acidemia (MMA), propionic acidemia (PA) or vitamin B₁₂ deficiency patients. On the other hand, MRS and NMR provide complementary information which is very valuable in the assessment of the severity of disease and efficiency of its treatment. Thus, MRS provides intracellular metabolic information from localized regions of the brain, while NMR provides extracellular metabolic information from biological fluids like urine, blood or cerebrospinal fluid. This paper presents an up-to-date review of the NMR and MRS studies reported to date for methylmalonic and propionic acidemias. Vitamin B₁₂ deficiency, although in most of its cases not inherited, shares similarities in its metabolic effects with MMA and it is also covered in this review. Full article

The development of turn-based inhibitors of protein–protein interactions has attracted considerable attention in medicinal chemistry. Our group has synthesized a series of peptides derived from an amino-functionalized ferrocene to investigate their potential to mimic protein turn structures. Detailed DFT and spectroscopic studies (IR, NMR, CD) have shown that, for peptides, the backbone chirality and bulkiness of the amino acid side chains determine the hydrogen-bond pattern, allowing tuning of the size of the preferred hydrogen-bonded ring in turn-folded structures. However, their biological potential is more dependent on their lipophilicity. In addition, our pioneering work on the chiroptical properties of aminoferrocene-containing peptides enables the correlation of their geometry with the sign of the CD signal in the absorption region of the ferrocene chromophore. These studies have opened up the possibility of using aminoferrocene and its derivatives as chirooptical probes for the determination of various chirality elements, such as the central chirality of amino acids and the helicity of peptide sequences. Full article

Resource Constraint Project Scheduling Problems with Discounted Cash Flows (RCPSPDC) focuses on maximizing the net present value by summing the discounted cash flows of project activities. An extension of this problem is the Payment at Event Occurrences (PEO) scheme, where the client makes multiple payments to the contractor upon completion of predefined activities, with additional final settlement at project completion. Numerous approximation methods such as metaheuristics have been proposed to solve this NP-hard problem. However, these methods suffer from parameter control and/or the computational cost of correcting infeasible solutions. Alternatively, approximate dynamic programming (ADP) sequentially generates a schedule based on strategies computed via Monte Carlo (MC) simulations. This saves the computations required for solution corrections, but its performance is highly dependent on its strategy. In this study, we propose the hybridization of ADP with three different metaheuristics to take advantage of their combined strengths, resulting in six different models. The Estimation of Distribution Algorithm (EDA) and Ant Colony Optimization (ACO) were used to recommend policies for ADP. A Discrete cCuckoo Search (DCS) further improved the schedules generated by ADP. Our experimental analysis performed on the j30, j60, and j90 datasets of PSPLIB has shown that ADP–DCS is better than ADP alone. Implementing the EDA and ACO as prioritization strategies for Monte Carlo simulations greatly improved the solutions with high statistical significance. In addition, models with the EDA showed better performance than those with ACO and random priority, especially when the number of events increased. Full article

To determine the effects of mat Pilates training on blood pressure, inflammatory, and antioxidative markers in hypertensive elderly people, 34 hypertensive subjects aged 60–75 years were randomly divided into a control group (CON; n = 17) and a mat Pilates training group (MP; n = 17). The CON participants conducted normal daily activities and participated in neither organized exercises nor sports training, while those in the MP group received mat Pilates training for 60 min three times/week for 12 weeks. Parameters including blood pressure, cardiovascular function, nitric oxide (NO), tumor necrotic factor-alpha (TNF-α), superoxide dismutase (SOD), and malonaldehyde (MDA) were collected at baseline and the end of 12 weeks. The MP group had significantly decreased blood pressure, improved cardiovascular variables, decreased MDA and TNF-α, and increased NO and SOD compared with the CON group and the pre-training period (p < 0.05). In conclusion, these findings demonstrate the positive effects of 12 weeks of mat Pilates training in terms of reducing blood pressure and increasing blood flow related to improvements in anti-inflammatory and antioxidative markers in hypertensive elderly people. Mat Pilates training might be integrated as an alternative therapeutic exercise modality in clinical practice for hypertensive elderly individuals. Full article

In this paper, we constructed a new and robust fixed point iterative scheme called the UO iterative scheme for the approximation of a contraction mapping. The scheme converges strongly to the fixed point of a contraction mapping. A rate of convergence result is shown with an example, and our scheme, when compared, converges faster than some existing iterative schemes in the literature. Furthermore, the stability and data dependence results are shown. Our new scheme is applied in the approximation of the solution to the oxygen diffusion model. Finally, our results are applied in the approximation of the solution to the boundary value problems using Green’s functions with an example. Full article

We consider a nine-parameter familiy of 3D quadratic systems, $\dot{x}=x+P_2(x,y,z), \dot{y}=-y+Q_2(x,y,z), \dot{z}=-z+R_2(x,y,z)$, where $P_2,Q_2,R_2$ are quadratic polynomials, in terms of integrability. We find necessary and sufficient conditions for the existence of two independent first integrals of corresponding semi-persistent, weakly persistent, and persistent systems. Unlike some of the earlier works, which primarily focus on planar systems, our research covers three-dimensional spaces, offering new insights into the complex dynamics that are not typically apparent in lower dimensions. Full article

With China’s rapid industrialization and urbanization in the process of socio-economic development, the extensive use of energy has resulted in a large amount of CO₂ emissions, which puts great pressure on China’s carbon emission reduction task. Through multivariate socio-economic data, this paper proposes an extraction and screening method of multivariate variables based on land-use types, and the downscaled spatial decomposition of carbon emissions at different scales was carried out by using the spatial lag model (SLM). This paper makes up for the shortcomings of previous studies, such as an insufficient modeling scale, simple modeling variables, limited spatio-temporal span of spatial decomposition, and no consideration of geographical correlation. Based on the results of the spatial decomposition of carbon emissions, this paper explores the spatial and temporal dynamics of carbon emissions at different scales. The results showed that SLM is capable of downscaling the spatialization of carbon emissions with high precision, and the continuity of the decomposition results at the provincial scale is stronger, while the differences of the decomposition results at the municipal scale are more obvious within the municipal units. In terms of the spatial and temporal dynamics of CO₂ emissions, carbon emissions at both scales showed a significant positive correlation. The dominant spatial correlation types are “Low–Low” at the provincial level, and “Low–Low” and “High–High” at the municipal level. The smaller spatial scope is more helpful to show the geographic dependence and geographic differences of China’s carbon emissions. The findings of this paper will help deepen the understanding of the spatial and temporal changes of carbon emissions in China. They will provide a scientific basis for the formulation of feasible carbon emission reduction policies. Full article

SiO₂ has a much higher theoretical specific capacity (1965 mAh g⁻¹) than graphite, making it a promising anode material for lithium-ion batteries, but its low conductivity and volume expansion problems need to be improved urgently. In this work, pompon mum-like SiO₂/C nanospheres with the sandwich and porous nanostructure were obtained by using dendritic fibrous nano silica (DFNS) and glucose as matrix and carbon source, respectively, through hydrothermal, carbonization and etching operations. The influence of SiO₂ content and porous structure on its electrochemical performance was discussed in detail. The final results showed that the C/DFNS-6 with a SiO₂ content of 6 wt% exhibits the best electrochemical performance as a negative electrode material for lithium-ion batteries due to its optimal specific surface area, porosity, and appropriate SiO₂ content. C/DFNS-6 displays a high specific reversible capacity of 986 mAh g⁻¹ at 0.2 A g⁻¹ after 200 cycles, and 529 mAh g⁻¹ at a high current density (1.0 A g⁻¹) after 300 cycles. It also has excellent rate capability, with a reversible capacity that rises from 599 mAh g⁻¹ to 1066 mAh g⁻¹ when the current density drops from 4.0 A g⁻¹ to 0.2 A g⁻¹. These SiO₂/C specific pompon mum-like nanospheres with excellent electrochemical performance have great research significance in the field of lithium-ion batteries. Full article

Antisense oligonucleotides (ASOs) are short oligodeoxynucleotides designed to bind to specific regions of target mRNA. ASOs can modulate pre-mRNA splicing, increase levels of functional proteins, and decrease levels of toxic proteins. ASOs are being developed for the treatment of motor neuron diseases (MNDs), including spinal muscular atrophy (SMA), amyotrophic lateral sclerosis (ALS) and spinal and bulbar muscular atrophy (SBMA). The biggest success has been the ASO known as nusinersen, the first effective therapy for SMA, able to improve symptoms and slow disease progression. Another success is tofersen, an ASO designed to treat ALS patients with SOD1 gene mutations. Both ASOs have been approved by the FDA and EMA. On the other hand, ASO treatment in ALS patients with the C9orf72 gene mutation did not show any improvement in disease progression. The aim of this review is to provide an up-to-date overview of ASO research in MNDs, from preclinical studies to clinical trials and, where available, regulatory approval. We highlight the successes and failures, underline the strengths and limitations of the current ASO research, and suggest possible approaches that could lead to more effective treatments. Full article

As a classical combinatorial optimization problem, the traveling salesman problem (TSP) has been extensively investigated in the fields of Artificial Intelligence and Operations Research. Due to being NP-complete, it is still rather challenging to solve both effectively and efficiently. Because of its high theoretical significance and wide practical applications, great effort has been undertaken to solve it from the point of view of intelligent search. In this paper, we propose a two-stage probe-based search optimization algorithm for solving both symmetric and asymmetric TSPs through the stages of route development and a self-escape mechanism. Specifically, in the first stage, a reasonable proportion threshold filter of potential basis probes or partial routes is set up at each step during the complete route development process. In this way, the poor basis probes with longer routes are filtered out automatically. Moreover, four local augmentation operators are further employed to improve these potential basis probes at each step. In the second stage, a self-escape mechanism or operation is further implemented on the obtained complete routes to prevent the probe-based search from being trapped in a locally optimal solution. The experimental results on a collection of benchmark TSP datasets demonstrate that our proposed algorithm is more effective than other state-of-the-art optimization algorithms. In fact, it achieves the best-known TSP benchmark solutions in many datasets, while, in certain cases, it even generates solutions that are better than the best-known TSP benchmark solutions. Full article

Human patients with type 1 diabetes mellitus (T1DM) are susceptible to several long-term complications that are related to glycemic control and immune dysregulation. Immune function remains relatively unexplored in dogs with naturally occurring diabetes mellitus (NODM). Calcitriol improves various aspects of immune function in a variety of species, but its effect in diabetic dogs remains unexplored. Therefore, the objectives of this study were to (i) evaluate immune function in dogs with NODM and determine if differences exist based on the level of clinical control and (ii) assess the immunomodulatory effects of calcitriol. Twenty diabetic dogs (clinically controlled, n = ten, not controlled, n = ten) and 20 non-diabetic, healthy control dogs were included in this prospective, case–control study. Whole blood was incubated with calcitriol (10⁻⁷ M) or negative control, after which the samples were divided for phagocytosis and leukocyte cytokine response experiments. The phagocytosis of opsonized Escherichia coli (E. coli) was evaluated with flow cytometry. The samples for leukocyte cytokine response evaluations were stimulated with lipopolysaccharide (LPS), lipoteichoic acid (LTA), or phosphate buffer solution (PBS; negative control), and tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, and IL-10 were measured in supernatant using a canine-specific multiplex bead-based assay. The leukocytes from diabetic dogs produced higher concentrations of IL-10 (p = 0.01), IL-6 (p < 0.0001), and IL-8 (p < 0.0001) than the control dogs while controlling for the intervention and stimulant. Calcitriol decreased the supernatant concentrations of TNF-α (p < 0.001) and IL-8 (p = 0.04) with concomitant increases in IL-6 (p = 0.005). Diabetic dogs had a lower percentage of leukocytes undergoing phagocytosis (p < 0.0001) but a higher number of bacteria phagocytized per cell (p = 0.001) when compared to the control dogs. Calcitriol had no effect on phagocytic capacity. Lastly, the status of clinical control in diabetic dogs did not yield differences in immune function. These results support that dogs with NODM exhibit immune dysregulation and warrant additional investigation. Full article

This paper interrogates the skepticism surrounding comparative ethics, particularly the question of its relevance in a world where ethical decision-making processes are primarily presumed to be dictated by one universalist culture. The paper argues that all cultures are inherently intercultural, evidenced by the historical coexistence of ideas and practices. Post-comparative ethics, which emphasizes the situational application of intellectual comparison and integration, is inevitable for postcolonial, non-Western societies. Historically, societies have navigated a variety of ethical frameworks, with some, like medieval Chinese society, embracing a plurality of beliefs. This pluralism is exemplified by the harmonious accommodation (yuanrong 圓融) of Confucianism, Daoism, and Buddhism. Using the example of Song Dynasty Chan master Dahui Zonggao 大慧宗杲, this article illustrates that intercultural ethics can be both diverse and sincere. Dahui’s pluralistic approach demonstrates that sincere commitment to multiple ethical systems is possible in our multicultural situation. I will discuss common approaches to the multicultural situation, such as expedient synthesis, theoretical synthesis, and crude syncretism, before illustrating the advantage of Dahui’s kanhua 看話禪method as harmonious accommodation through confrontation. This underscores the importance of shifting the debate from “Why compare?” to “How to compare?” in achieving the accommodation of different ethical frameworks. Full article

Sorghum (Sorghum bicolour (L.) Moench) is an emerging reliable alternative for mahewu production. The aim of this study was to evaluate the health-promoting compounds, physicochemical and microbiological properties of sorghum-based mahewu produced by different traditional brewers in Thohoyandou, South Africa. A total of 18 mahewu samples produced by six traditional brewers (TB1–TB6) were collected and compared for antioxidant, physicochemical, and microbiological properties. Commercial sorghum mahewu was used as a control sample. The total phenolic content of the mahewu samples varied from 27.37 to 65.89 GAE /g, with commercial mahewu having a lower value. The flavonoid content ranged from 0.18 to 0.30 GAE/g, and commercial mahewu had a higher value. The DPPH scavenging activity and FRAP of mahewu samples ranged from 44.62% to 49% and 1.47 to 2.36 mg GAE/g, respectively. Commercial mahewu had a higher DPPH value but a lower FRAP value. The pH of mahewu varied significantly, ranging from 3.38 to 3.66, but was within the acceptable range. The °Brix values varied from 9.68 to 17.49, with traditional mahewu samples having higher values than commercial mahewu. Total titratable acidity ranged from 0.63 to 1.17%. The viscosity ranged from 444.33 to 1297.00 cP, with commercial mahewu having a higher value. There was a significant variation in the color of the mahewu samples with respect to L*, a*, b*, C, Hue, and ΔΕ. The growth of yeasts and molds varied from 7.95 log₁₀ to 8.99 log₁₀ (cfu/mL) in traditional mahewu samples, and coliforms ranged from 3.68 to 5.96 log₁₀ (cfu/mL) and were not isolated in commercial mahewu. The total plate count ranged from 7.914 to 8.978 log₁₀ (cfu/mL). The microbiological results show that traditional brewers are meeting the legal limit and can increase their products for commercialization. Full article