The 2023 MDPI Annual Report has
been released!
 
Article
Light as a Form of Visual Language Supporting Daily Schedules in Educational Spaces: A Design Framework
by Stavroula Angelaki and Georgios A. Triantafyllidis
Buildings 2024, 14(5), 1385; https://doi.org/10.3390/buildings14051385 (registering DOI) - 12 May 2024
Abstract
This study explores how lighting can be employed as a visual language to enhance communication between the space and its users and develop a design framework for educational spaces. A primary school is used as a case study to apply the proposed design [...] Read more.
This study explores how lighting can be employed as a visual language to enhance communication between the space and its users and develop a design framework for educational spaces. A primary school is used as a case study to apply the proposed design framework. The study focuses on lighting interventions in existing educational spaces to support daily schedules and transitions between activities. In this context, electric light is used as an indicator, highlighting the daily schedule and activities in the space. A theoretical approach is used as a foundation for establishing the design framework that leads to lighting proposals based on the specific spatial characteristics of each study. The outcome is a design solution based on the dominant spatial elements that define the space’s identity and function. The study focuses on educational spaces and lighting for peripheral vision while considering pupils’ visual and spatial development. The proposal has the role of an additional light layer that signals transitions in terms of activities or spatial mobility. Full article
(This article belongs to the Special Issue Lighting in Buildings—2nd Edition)
18 pages, 4270 KiB  
Article
Persistent Mesodermal Differentiation Capability of Bone Marrow MSCs Isolated from Aging Patients with Low-Energy Traumatic Hip Fracture and Osteoporosis: A Clinical Evidence
by Mei-Chih Wang, Wei-Lin Yu, Yun-Chiao Ding, Jun-Jae Huang, Chin-Yu Lin and Wo-Jan Tseng
Int. J. Mol. Sci. 2024, 25(10), 5273; https://doi.org/10.3390/ijms25105273 (registering DOI) - 12 May 2024
Abstract
A low-energy hit, such as a slight fall from a bed, results in a bone fracture, especially in the hip, which is a life-threatening risk for the older adult and a heavy burden for the social economy. Patients with low-energy traumatic bone fractures [...] Read more.
A low-energy hit, such as a slight fall from a bed, results in a bone fracture, especially in the hip, which is a life-threatening risk for the older adult and a heavy burden for the social economy. Patients with low-energy traumatic bone fractures usually suffer a higher level of bony catabolism accompanied by osteoporosis. Bone marrow-derived stem cells (BMSCs) are critical in osteogenesis, leading to metabolic homeostasis in the healthy bony microenvironment. However, whether the BMSCs derived from the patients who suffered osteoporosis and low-energy traumatic hip fractures preserve a sustained mesodermal differentiation capability, especially in osteogenesis, is yet to be explored in a clinical setting. Therefore, we aimed to collect BMSCs from clinical hip fracture patients with osteoporosis, followed by osteogenic differentiation comparison with BMSCs from healthy young donors. The CD markers identification, cytokines examination, and adipogenic differentiation were also evaluated. The data reveal that BMSCs collected from elderly osteoporotic patients secreted approximately 122.8 pg/mL interleukin 6 (IL-6) and 180.6 pg/mL vascular endothelial growth factor (VEGF), but no PDGF-BB, IL-1b, TGF-b1, IGF-1, or TNF-α secretion. The CD markers and osteogenic and adipogenic differentiation capability in BMSCs from these elderly osteoporotic patients and healthy young donors are equivalent and compliant with the standards defined by the International Society of Cell Therapy (ISCT). Collectively, our data suggest that the elderly osteoporotic patients-derived BMSCs hold equivalent differentiation and proliferation capability and intact surface markers identical to BMSCs collected from healthy youth and are available for clinical cell therapy. Full article
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18 pages, 2572 KiB  
Article
Degradation of Bisphenol A by Nitrogen-Rich ZIF-8-Derived Carbon Materials-Activated Peroxymonosulfate
by Xiaofeng Tang, Hanqing Xue, Jiawen Li, Shengnan Wang, Jie Yu and Tao Zeng
Toxics 2024, 12(5), 359; https://doi.org/10.3390/toxics12050359 (registering DOI) - 12 May 2024
Abstract
Bisphenol A (BPA), representing a class of organic pollutants, finds extensive applications in the pharmaceutical industry. However, its widespread use poses a significant hazard to both ecosystem integrity and human health. Advanced oxidation processes (AOPs) based on peroxymonosulfate (PMS) via heterogeneous catalysts are [...] Read more.
Bisphenol A (BPA), representing a class of organic pollutants, finds extensive applications in the pharmaceutical industry. However, its widespread use poses a significant hazard to both ecosystem integrity and human health. Advanced oxidation processes (AOPs) based on peroxymonosulfate (PMS) via heterogeneous catalysts are frequently proposed for treating persistent pollutants. In this study, the degradation performance of BPA in an oxidation system of PMS activated by transition metal sites anchored nitrogen-doped carbonaceous substrate (M-N-C) materials was investigated. As heterogeneous catalysts targeting the activation of peroxymonosulfate (PMS), M-N-C materials emerge as promising contenders poised to overcome the limitations encountered with traditional carbon materials, which often exhibit insufficient activity in the PMS activation process. Nevertheless, the amalgamation of metal sites during the synthesis process presents a formidable challenge to the structural design of M-N-C. Herein, employing ZIF-8 as the precursor of carbonaceous support, metal ions can readily penetrate the cage structure of the substrate, and the N-rich linkers serve as effective ligands for anchoring metal cations, thereby overcoming the awkward limitation. The research results of this study indicate BPA in water matrix can be effectively removed in the M-N-C/PMS system, in which the obtained nitrogen-rich ZIF-8-derived Cu-N-C presented excellent activity and stability on the PMS activation, as well as the outstanding resistance towards the variation of environmental factors. Moreover, the biological toxicity of BPA and its degradation intermediates were investigated via the Toxicity Estimation Software Tool (T.E.S.T.) based on the ECOSAR system. Full article
(This article belongs to the Section Toxicity Reduction and Environmental Remediation)
19 pages, 6791 KiB  
Article
Hypermethylation of the Gene Body in SRCIN1 Is Involved in Breast Cancer Cell Proliferation and Is a Potential Blood-Based Biomarker for Early Detection and a Poor Prognosis
by Hsieh-Tsung Shen, Chin-Sheng Hung, Clilia Davis, Chih-Ming Su, Li-Min Liao, Hsiu-Ming Shih, Kuan-Der Lee, Muhamad Ansar and Ruo-Kai Lin
Biomolecules 2024, 14(5), 571; https://doi.org/10.3390/biom14050571 (registering DOI) - 12 May 2024
Abstract
Breast cancer is a leading cause of cancer mortality in women worldwide. Using the Infinium MethylationEPIC BeadChip, we analyzed plasma sample methylation to identify the SRCIN1 gene in breast cancer patients. We assessed SRCIN1-related roles and pathways for their biomarker potential. To [...] Read more.
Breast cancer is a leading cause of cancer mortality in women worldwide. Using the Infinium MethylationEPIC BeadChip, we analyzed plasma sample methylation to identify the SRCIN1 gene in breast cancer patients. We assessed SRCIN1-related roles and pathways for their biomarker potential. To verify the methylation status, quantitative methylation-specific PCR (qMSP) was performed on genomic DNA and circulating cell-free DNA samples, and mRNA expression analysis was performed using RT‒qPCR. The results were validated in a Western population; for this analysis, the samples included plasma samples from breast cancer patients from the USA and from The Cancer Genome Atlas (TCGA) cohort. To study the SRCIN1 pathway, we conducted cell viability assays, gene manipulation and RNA sequencing. SRCIN1 hypermethylation was identified in 61.8% of breast cancer tissues from Taiwanese patients, exhibiting specificity to this malignancy. Furthermore, its presence correlated significantly with unfavorable 5-year overall survival outcomes. The levels of methylated SRCIN1 in the blood of patients from Taiwan and the USA correlated with the stage of breast cancer. The proportion of patients with high methylation levels increased from 0% in healthy individuals to 63.6% in Stage 0, 80% in Stage I and 82.6% in Stage II, with a sensitivity of 78.5%, an accuracy of 90.3% and a specificity of 100%. SRCIN1 hypermethylation was significantly correlated with increased SRCIN1 mRNA expression (p < 0.001). Knockdown of SRCIN1 decreased the viability of breast cancer cells. SRCIN1 silencing resulted in the downregulation of ESR1, BCL2 and various cyclin protein expressions. SRCIN1 hypermethylation in the blood may serve as a noninvasive biomarker, facilitating early detection and prognosis evaluation, and SRCIN1-targeted therapies could be used in combination regimens for breast cancer patients. Full article
(This article belongs to the Special Issue DNA Methylation in Human Diseases)
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23 pages, 12176 KiB  
Article
Broken Rotor Bar Detection Based on Steady-State Stray Flux Signals Using Triaxial Sensor with Random Positioning
by Marko Zubčić, Ivan Pavić, Petar Matić and Adam Polak
Sensors 2024, 24(10), 3080; https://doi.org/10.3390/s24103080 (registering DOI) - 12 May 2024
Abstract
This paper investigates the detection of broken rotor bar in squirrel cage induction motors using a novel approach of randomly positioning a triaxial sensor over the motor surface. This study is conducted on two motors under laboratory conditions, where one motor is kept [...] Read more.
This paper investigates the detection of broken rotor bar in squirrel cage induction motors using a novel approach of randomly positioning a triaxial sensor over the motor surface. This study is conducted on two motors under laboratory conditions, where one motor is kept in a healthy state, and the other is subjected to a broken rotor bar (BRB) fault. The induced electromotive force of the triaxial coils, recorded over ten days with 100 measurements per day, is statistically analyzed. Normality tests and graphical interpretation methods are used to evaluate the data distribution. Parametric and non-parametric approaches are used to analyze the data. Both approaches show that the measurement method is valid and consistent over time and statistically distinguishes healthy motors from those with BRB defects when a reference or threshold value is specified. While the comparison between healthy motors shows a discrepancy, the quantitative analysis shows a smaller estimated difference in mean values between healthy motors than comparing healthy and BRB motors. Full article
(This article belongs to the Section Physical Sensors)
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21 pages, 1180 KiB  
Article
Extracting Accurate Parameters from a Proton Exchange Membrane Fuel Cell Model Using the Differential Evolution Ameliorated Meta-Heuristics Algorithm
by Badreddine Kanouni and Abdelbaset Laib
Energies 2024, 17(10), 2333; https://doi.org/10.3390/en17102333 (registering DOI) - 12 May 2024
Abstract
The electrochemical proton exchange membrane fuel cell (PEMFC) is an electrical generator that utilizes a chemical reaction mechanism to produce electricity, serving as a sustainable and environmentally friendly energy source. To thoroughly analyze and develop the features and performance of a PEMFC, it [...] Read more.
The electrochemical proton exchange membrane fuel cell (PEMFC) is an electrical generator that utilizes a chemical reaction mechanism to produce electricity, serving as a sustainable and environmentally friendly energy source. To thoroughly analyze and develop the features and performance of a PEMFC, it is essential to use a precise model that incorporates exact parameters to effectively suit the polarization curve. In addition, parameter extraction plays a crucial role in the simulation analysis, evaluation, optimum control, and fault detection of the proton exchange membrane fuel cell (PEMFC) system. Despite the development of many algorithms for parameter extraction in PEMFC, obtaining accurate and trustworthy results rapidly remains a challenge. This study presents a hybridized algorithm, namely differential evolution ameliorated (DEA) for reliably estimating PEMFC model parameters. To evaluate the proposed DEA-based parameter identification, a comparison analysis with previously published methods is conducted using MATLAB/SimulinkTM (R2016b, MathWorks, Natick, MA, USA) in terms of system correctness and convergence process. The proposed DEA algorithm is tested to extract the parameters of two PEMFC models: SR-12,500 W and 250 W. The sum of the squared errors (SSE) between the experimental and the obtained voltage data is defined as an objective function. The simulation results prove that the suggested DEA algorithm is capable of identifying the optimal PEMFC parameters rapidly and accurately in comparison with other optimization algorithms. Full article
16 pages, 4105 KiB  
Article
Characterization of the Plastid Genomes of Four Caroxylon Thunb. Species from Kazakhstan
by Shyryn Almerekova, Moldir Yermagambetova, Bektemir Osmonali, Polina Vesselova, Saule Abugalieva and Yerlan Turuspekov
Plants 2024, 13(10), 1332; https://doi.org/10.3390/plants13101332 (registering DOI) - 12 May 2024
Abstract
The family Chenopodiaceae Vent. (Amaranthaceae s.l.) is known for its taxonomic complexity, comprising species of significant economic and ecological importance. Despite its significance, the availability of plastid genome data for this family remains limited. This study involved assembling and characterizing the complete [...] Read more.
The family Chenopodiaceae Vent. (Amaranthaceae s.l.) is known for its taxonomic complexity, comprising species of significant economic and ecological importance. Despite its significance, the availability of plastid genome data for this family remains limited. This study involved assembling and characterizing the complete plastid genomes of four Caroxylon Thunb. species within the tribe Salsoleae s.l., utilizing next-generation sequencing technology. We compared genome features, nucleotide diversity, and repeat sequences and conducted a phylogenetic analysis of ten Salsoleae s.l. species. The size of the plastid genome varied among four Caroxylon species, ranging from 150,777 bp (C. nitrarium) to 151,307 bp (C. orientale). Each studied plastid genome encoded 133 genes, including 114 unique genes. This set of genes includes 80 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Eight divergent regions (accD, atpF, matK, ndhF-ndhG, petB, rpl20-rpl22, rpoC2, and ycf3) were identified in ten Salsoleae s.l. plastid genomes, which could be potential DNA-barcoding markers. Additionally, 1106 repeat elements were detected, consisting of 814 simple sequence repeats, 92 tandem repeats, 88 forward repeats, 111 palindromic repeats, and one reverse repeat. The phylogenetic analysis provided robust support for the relationships within Caroxylon species. These data represent a valuable resource for future phylogenetic studies within the genus. Full article
(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
32 pages, 1183 KiB  
Review
Live Attenuated Vaccines against Tuberculosis: Targeting the Disruption of Genes Encoding the Secretory Proteins of Mycobacteria
by Raja Veerapandian, Shrikanth S. Gadad, Chinnaswamy Jagannath and Subramanian Dhandayuthapani
Vaccines 2024, 12(5), 530; https://doi.org/10.3390/vaccines12050530 (registering DOI) - 12 May 2024
Abstract
Tuberculosis (TB), a chronic infectious disease affecting humans, causes over 1.3 million deaths per year throughout the world. The current preventive vaccine BCG provides protection against childhood TB, but it fails to protect against pulmonary TB. Multiple candidates have been evaluated to either [...] Read more.
Tuberculosis (TB), a chronic infectious disease affecting humans, causes over 1.3 million deaths per year throughout the world. The current preventive vaccine BCG provides protection against childhood TB, but it fails to protect against pulmonary TB. Multiple candidates have been evaluated to either replace or boost the efficacy of the BCG vaccine, including subunit protein, DNA, virus vector-based vaccines, etc., most of which provide only short-term immunity. Several live attenuated vaccines derived from Mycobacterium tuberculosis (Mtb) and BCG have also been developed to induce long-term immunity. Since Mtb mediates its virulence through multiple secreted proteins, these proteins have been targeted to produce attenuated but immunogenic vaccines. In this review, we discuss the characteristics and prospects of live attenuated vaccines generated by targeting the disruption of the genes encoding secretory mycobacterial proteins. Full article
(This article belongs to the Special Issue Novel Vaccines for Infectious Pathogens)
17 pages, 692 KiB  
Article
Clinical Neurophysiological Methods Verify Improvement in the Motor Neural Transmission in Patients with Surgically Treated Idiopathic Scoliosis in Long-Term Follow-up
by Przemysław Daroszewski, Juliusz Huber, Katarzyna Kaczmarek, Piotr Janusz, Paweł Główka, Marek Tomaszewski, Łukasz Kubaszewski, Mikołaj Dąbrowski and Tomasz Kotwicki
Appl. Sci. 2024, 14(10), 4105; https://doi.org/10.3390/app14104105 (registering DOI) - 12 May 2024
Abstract
The evaluation of patients after the surgical correction of idiopathic scoliosis in a long-term follow-up with clinical neurophysiological methods has not been presented in detail. This study aimed to compare the results of neurophysiological studies in 45 girls with scoliosis of Lenke types [...] Read more.
The evaluation of patients after the surgical correction of idiopathic scoliosis in a long-term follow-up with clinical neurophysiological methods has not been presented in detail. This study aimed to compare the results of neurophysiological studies in 45 girls with scoliosis of Lenke types 1–3 performed pre- (T0) and postoperatively, 1 week after surgery (T1) and 6 months after surgery (T2). The parameter values of the surface electromyography while attempting maximal contraction (mcsEMG) and the transcranial motor-evoked potentials (MEPs) recorded in the anterior tibial muscles, as well as the electroneurography (ENG) of the peripheral transmission in the peroneal nerve motor fibers, were compared. The results indicate that efferent neural conduction functioned both centrally and peripherally, and TA muscle function slightly improved immediately after the surgical correction of scoliosis, and further normalization appeared after six months in the long-term follow-up (at p = 0.03). The sEMG recordings indicate that half a year after surgical treatment in IS patients, the TA muscle motor unit recruitment function, as well as the muscle strength evaluated with Lovett’s scale, was comparable to the normal condition. The ENG recording results indicate a gradual reduction in the motor fiber injury symptoms, mainly of the axonal type, in the peroneal nerves. The surgeries also improved the lumbar ventral roots’ neural transmission to a normal functional status. The MEP amplitude parameter values recorded after the surgical scoliosis corrections in T1 indicated a slight improvement in the efferent transmission of neural impulses within the fibers of the spinal tracts; in the long-term T2 observation period, they reached values comparable to those recorded in healthy volunteers, bilaterally. Preoperatively (T0), the results of all the neurophysiological study parameters in the IS patients were asymmetrical at p = 0.036–0.05 and recorded as worse on the concave side, suggesting the lateralization of neurological motor deficits. One week postoperatively (T1), this asymmetry was recorded as gradually reduced, showing almost no difference between the right and left sides six months later (T2). The presented algorithm for the neurophysiological assessments performed in the pre-, intra-, and long-term postoperative periods using the mcsEMG, MEP, and ENG neurophysiological examinations, together with the clinical studies, may help in the comprehensive functional evaluation of the spinal cord tracts and ventral root neural conduction, which allows the detection of the subclinical neurological changes related to scoliosis itself and the consequences of the corrective surgery. Such an evaluation can also be significant in making final decisions regarding IS surgeries and their personalization after attempting conservative treatments with bracing and kinesiotherapy. Neurophysiological studies, as a sensitive biomarker, allowed us to predict and ascertain the final result of IS treatment in the long-term follow-up, which showed the health status of patients as being comparable to that of healthy volunteers. Full article
(This article belongs to the Section Applied Biosciences and Bioengineering)
17 pages, 1113 KiB  
Article
Seasonal Variation in Short-Term Ambient Air Pollutants and ST-Elevation Myocardial Infarction Admissions: An Innovative Exploration of Air Pollution’s Health Consequences
by Andreea-Alexandra Rus, Raluca Şoşdean, Mihai-Andrei Lazăr, Marius Simonescu, Silvia-Ana Luca, Ciprian Nicuşor Dima, Alexandra-Cătălina Frişan, Dan Gaiţă and Cristian Mornoş
Atmosphere 2024, 15(5), 590; https://doi.org/10.3390/atmos15050590 (registering DOI) - 12 May 2024
Abstract
Cardiovascular diseases (CVDs) persist as a significant contributor to global morbidity and mortality despite advances in medical technology. Air pollution has emerged as a significant contemporary challenge due to increased energy consumption and rapid economic development. The study utilized multivariable Poisson regression and [...] Read more.
Cardiovascular diseases (CVDs) persist as a significant contributor to global morbidity and mortality despite advances in medical technology. Air pollution has emerged as a significant contemporary challenge due to increased energy consumption and rapid economic development. The study utilized multivariable Poisson regression and Distributed Lag Models (DLM) to assess the link between brief exposure to outdoor air pollutants (PM10—particulate matter with a diameter ≤ 10 μm, NO2—nitrogen dioxide, and O3—ozone) and the risk of acute myocardial infarction with ST-segment elevation (STEMI) hospitalization, stratified by season. The research was conducted from January 2019 to December 2021 at the University Hospital in Timisoara, Romania, and daily records were collected for STEMI admissions, atmospheric pollutant levels, and meteorological parameters. The most pronounced impacts were observed with each 10 μg/m3 increase at lag 07 for PM10 during summer, leading to a 2% increase in STEMI admissions, and for NO2 during spring at lag 07, resulting in a 0.9% rise in CVD incidence. Men, middle-aged adults, and older adults exhibited greater susceptibility to elevated NO2 and PM10 concentrations than women and younger individuals. Brief exposure to diverse air pollutants heightens the likelihood of hospitalization due to STEMI, particularly among men and adults over 45. Effective measures must be implemented to mitigate these impacts, especially for vulnerable populations. Full article
(This article belongs to the Special Issue New Insights into Exposure and Health Impacts of Air Pollution)
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13 pages, 656 KiB  
Article
Periodic Solutions in a Simple Delay Differential Equation
by Anatoli Ivanov and Sergiy Shelyag
Math. Comput. Appl. 2024, 29(3), 36; https://doi.org/10.3390/mca29030036 (registering DOI) - 12 May 2024
Abstract
A simple-form scalar differential equation with delay and nonlinear negative periodic feedback is considered. The existence of several types of slowly oscillating periodic solutions is shown with the same and double periods of the feedback coefficient. The periodic solutions are built explicitly in [...] Read more.
A simple-form scalar differential equation with delay and nonlinear negative periodic feedback is considered. The existence of several types of slowly oscillating periodic solutions is shown with the same and double periods of the feedback coefficient. The periodic solutions are built explicitly in the case with piecewise constant nonlinearities involved. The periodic dynamics are shown to persist under small perturbations of the equation, which make it smooth. The theoretical results are verified through extensive numerical simulations. Full article
22 pages, 7403 KiB  
Article
A Hybrid Semi-Automated Workflow for Systematic and Literature Review Processes with Large Language Model Analysis
by Anjia Ye, Ananda Maiti, Matthew Schmidt and Scott J. Pedersen
Future Internet 2024, 16(5), 167; https://doi.org/10.3390/fi16050167 (registering DOI) - 12 May 2024
Abstract
Systematic reviews (SRs) are a rigorous method for synthesizing empirical evidence to answer specific research questions. However, they are labor-intensive because of their collaborative nature, strict protocols, and typically large number of documents. Large language models (LLMs) and their applications such as gpt-4/ChatGPT [...] Read more.
Systematic reviews (SRs) are a rigorous method for synthesizing empirical evidence to answer specific research questions. However, they are labor-intensive because of their collaborative nature, strict protocols, and typically large number of documents. Large language models (LLMs) and their applications such as gpt-4/ChatGPT have the potential to reduce the human workload of the SR process while maintaining accuracy. We propose a new hybrid methodology that combines the strengths of LLMs and humans using the ability of LLMs to summarize large bodies of text autonomously and extract key information. This is then used by a researcher to make inclusion/exclusion decisions quickly. This process replaces the typical manually performed title/abstract screening, full-text screening, and data extraction steps in an SR while keeping a human in the loop for quality control. We developed a semi-automated LLM-assisted (Gemini-Pro) workflow with a novel innovative prompt development strategy. This involves extracting three categories of information including identifier, verifier, and data field (IVD) from the formatted documents. We present a case study where our hybrid approach reduced errors compared with a human-only SR. The hybrid workflow improved the accuracy of the case study by identifying 6/390 (1.53%) articles that were misclassified by the human-only process. It also matched the human-only decisions completely regarding the rest of the 384 articles. Given the rapid advances in LLM technology, these results will undoubtedly improve over time. Full article
(This article belongs to the Section Big Data and Augmented Intelligence)
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22 pages, 2035 KiB  
Review
The Emerging Role of Ubiquitin-Specific Protease 36 (USP36) in Cancer and Beyond
by Meng-Yao Niu, Yan-Jun Liu, Jin-Jin Shi, Ru-Yi Chen, Shun Zhang, Chang-Yun Li, Jia-Feng Cao, Guan-Jun Yang and Jiong Chen
Biomolecules 2024, 14(5), 572; https://doi.org/10.3390/biom14050572 (registering DOI) - 12 May 2024
Abstract
The balance between ubiquitination and deubiquitination is instrumental in the regulation of protein stability and maintenance of cellular homeostasis. The deubiquitinating enzyme, ubiquitin-specific protease 36 (USP36), a member of the USP family, plays a crucial role in this dynamic equilibrium by hydrolyzing and [...] Read more.
The balance between ubiquitination and deubiquitination is instrumental in the regulation of protein stability and maintenance of cellular homeostasis. The deubiquitinating enzyme, ubiquitin-specific protease 36 (USP36), a member of the USP family, plays a crucial role in this dynamic equilibrium by hydrolyzing and removing ubiquitin chains from target proteins and facilitating their proteasome-dependent degradation. The multifaceted functions of USP36 have been implicated in various disease processes, including cancer, infections, and inflammation, via the modulation of numerous cellular events, including gene transcription regulation, cell cycle regulation, immune responses, signal transduction, tumor growth, and inflammatory processes. The objective of this review is to provide a comprehensive summary of the current state of research on the roles of USP36 in different pathological conditions. By synthesizing the findings from previous studies, we have aimed to increase our understanding of the mechanisms underlying these diseases and identify potential therapeutic targets for their treatment. Full article
(This article belongs to the Special Issue Emerging Roles of Epigenetic Regulators in Inflammatory Diseases)
27 pages, 30061 KiB  
Article
Puerarin Modulates Hepatic Farnesoid X Receptor and Gut Microbiota in High-Fat Diet-Induced Obese Mice
by Ching-Wei Yang, Hsuan-Miao Liu, Zi-Yu Chang, Geng-Hao Liu, Hen-Hong Chang, Po-Yu Huang and Tzung-Yan Lee
Int. J. Mol. Sci. 2024, 25(10), 5274; https://doi.org/10.3390/ijms25105274 (registering DOI) - 12 May 2024
Abstract
Obesity is associated with alterations in lipid metabolism and gut microbiota dysbiosis. This study investigated the effects of puerarin, a bioactive isoflavone, on lipid metabolism disorders and gut microbiota in high-fat diet (HFD)-induced obese mice. Supplementation with puerarin reduced plasma alanine aminotransferase, liver [...] Read more.
Obesity is associated with alterations in lipid metabolism and gut microbiota dysbiosis. This study investigated the effects of puerarin, a bioactive isoflavone, on lipid metabolism disorders and gut microbiota in high-fat diet (HFD)-induced obese mice. Supplementation with puerarin reduced plasma alanine aminotransferase, liver triglyceride, liver free fatty acid (FFA), and improved gut microbiota dysbiosis in obese mice. Puerarin’s beneficial metabolic effects were attenuated when farnesoid X receptor (FXR) was antagonized, suggesting FXR-mediated mechanisms. In hepatocytes, puerarin ameliorated high FFA-induced sterol regulatory element-binding protein (SREBP) 1 signaling, inflammation, and mitochondrial dysfunction in an FXR-dependent manner. In obese mice, puerarin reduced liver damage, regulated hepatic lipogenesis, decreased inflammation, improved mitochondrial function, and modulated mitophagy and ubiquitin-proteasome pathways, but was less effective in FXR knockout mice. Puerarin upregulated hepatic expression of FXR, bile salt export pump (BSEP), and downregulated cytochrome P450 7A1 (CYP7A1) and sodium taurocholate transporter (NTCP), indicating modulation of bile acid synthesis and transport. Puerarin also restored gut microbial diversity, the Firmicutes/Bacteroidetes ratio, and the abundance of Clostridium celatum and Akkermansia muciniphila. This study demonstrates that puerarin effectively ameliorates metabolic disturbances and gut microbiota dysbiosis in obese mice, predominantly through FXR-dependent pathways. These findings underscore puerarin’s potential as a therapeutic agent for managing obesity and enhancing gut health, highlighting its dual role in improving metabolic functions and modulating microbial communities. Full article
(This article belongs to the Special Issue Gut Microbiota in Gastroenterology and Hepatology 2.0)
23 pages, 2173 KiB  
Article
Encoder–Decoder Based LSTM and GRU Architectures for Stocks and Cryptocurrency Prediction
by Joy Dip Das, Ruppa K. Thulasiram, Christopher Henry and Aerambamoorthy Thavaneswaran
J. Risk Financial Manag. 2024, 17(5), 200; https://doi.org/10.3390/jrfm17050200 (registering DOI) - 12 May 2024
Abstract
This work addresses the intricate task of predicting the prices of diverse financial assets, including stocks, indices, and cryptocurrencies, each exhibiting distinct characteristics and behaviors under varied market conditions. To tackle the challenge effectively, novel encoder–decoder architectures, AE-LSTM and AE-GRU, integrating the encoder–decoder [...] Read more.
This work addresses the intricate task of predicting the prices of diverse financial assets, including stocks, indices, and cryptocurrencies, each exhibiting distinct characteristics and behaviors under varied market conditions. To tackle the challenge effectively, novel encoder–decoder architectures, AE-LSTM and AE-GRU, integrating the encoder–decoder principle with LSTM and GRU, are designed. The experimentation involves multiple activation functions and hyperparameter tuning. With extensive experimentation and enhancements applied to AE-LSTM, the proposed AE-GRU architecture still demonstrates significant superiority in forecasting the annual prices of volatile financial assets from the multiple sectors mentioned above. Thus, the novel AE-GRU architecture emerges as a superior choice for price prediction across diverse sectors and fluctuating volatile market scenarios by extracting important non-linear features of financial data and retaining the long-term context from past observations. Full article
(This article belongs to the Special Issue Machine Learning Applications in Finance)
18 pages, 3113 KiB  
Article
Study on Microscopic Characteristics and Rock Mechanical Properties of Tight Sandstone after Acidification–Supercritical CO2 Composite Action: Case Study from Xujiahe Formation, China
by Yunfei Zhao, Gun Huang, Qinming Liang and Qiang Chen
Appl. Sci. 2024, 14(10), 4108; https://doi.org/10.3390/app14104108 (registering DOI) - 12 May 2024
Abstract
Acidified CO2 fracturing is a viable method for increasing production in deep, tight sandstone reservoirs. However, the potential mechanism of changes in pore structure and mechanical properties of sandstone under acidified CO2 supercritical composite is not clear. Understanding this mechanism is [...] Read more.
Acidified CO2 fracturing is a viable method for increasing production in deep, tight sandstone reservoirs. However, the potential mechanism of changes in pore structure and mechanical properties of sandstone under acidified CO2 supercritical composite is not clear. Understanding this mechanism is important for the study of crack initiation and extension in tight sandstone reservoirs. This study utilizes sandstone samples from the Xujiahe Formation reservoir in Rongchang District as experimental specimens. The primary focus is to analyze the changes in pore structure and mechanical properties of these samples after acidification–supercritical CO2 composite action. Nuclear magnetic resonance (NMR) and uniaxial compression tests are employed as the main investigative techniques. The results show that there was a physicochemical synergy between the acidification–supercritical CO2 composite effect; the crack initial stress, damage stress, and peak stress of the sandstone after 16 MPa supercritical CO2 acidification treatment were reduced by 20%, 49.5%, and 49.8%, respectively; the crack volumetric strain accelerated and the sandstone evolved from brittle to ductile damage; and the larger pore space and microcracks of the sandstone increased significantly after the treatment, which can be attributed to the gradual dissolution of intergranular cement leading to the formation of new pores connected to the existing pore network. The change mechanism of sandstone after acidification–supercritical CO2 compound treatment is also proposed. Full article
(This article belongs to the Special Issue Advances in Geo-Energy Development and Enhanced Oil/Gas Recovery)
16 pages, 7789 KiB  
Article
Local Path Planner for Mobile Robot Considering Future Positions of Obstacles
by Xianhua Ou, Zhongnan You and Xiongxiong He
Processes 2024, 12(5), 984; https://doi.org/10.3390/pr12050984 (registering DOI) - 12 May 2024
Abstract
Local path planning is a necessary ability for mobile robot navigation, but existing planners are not sufficiently effective at dynamic obstacle avoidance. In this article, an improved timed elastic band (TEB) planner based on the requirements of mobile robot navigation in dynamic environments [...] Read more.
Local path planning is a necessary ability for mobile robot navigation, but existing planners are not sufficiently effective at dynamic obstacle avoidance. In this article, an improved timed elastic band (TEB) planner based on the requirements of mobile robot navigation in dynamic environments is proposed. The dynamic obstacle velocities and TEB poses are fully integrated through two-dimensional (2D) lidar and multi-obstacle tracking. First, background point filtering and clustering are performed on the lidar points to obtain obstacle clusters. Then, we calculate the data association matrix of the obstacle clusters of the current and previous frame so that the clusters can be matched. Thirdly, a Kalman filter is adopted to track clusters and obtain the optimal estimates of their velocities. Finally, the TEB poses and obstacle velocities are associated: we predict the obstacle position corresponding to the TEB pose through the detected obstacle velocity and add this constraint to the corresponding TEB pose vertex. Then, a pose sequence considering the future positions of obstacles is obtained through a graph optimization algorithm. Compared with the original TEB, our method reduces the total running time by 22.87%, reduces the running distance by 19.23%, and increases the success rate by 21.05%. Simulations and experiments indicate that the improved TEB enables robots to efficiently avoid dynamic obstacles and reach the goal as quickly as possible. Full article
36 pages, 22878 KiB  
Article
Designing a Competency-Focused Course on Applied AI Based on Advanced System Research on Business Requirements
by Vasyl Martsenyuk, Georgi Dimitrov, Dejan Rancic, Iveta Dirgova Luptakova, Igor Jovancevic, Marcin Bernas, Aleksandra Klos-Witkowska, Tomasz Gancarczyk, Iva Kostadinova, Elizabet Mihaylova, Dragan Stojanovic, Marko Milojkovic, Jiri Pospichal and Aleksandar Plamenac
Appl. Sci. 2024, 14(10), 4107; https://doi.org/10.3390/app14104107 (registering DOI) - 12 May 2024
Abstract
The consortium of “The Future is in Applied Artificial Intelligence” Project designed the first competency-based applied artificial intelligence curriculum at the higher-education institution level. The development was based on advanced system research on existing artificial intelligence-related resources and surveying target groups of teachers, [...] Read more.
The consortium of “The Future is in Applied Artificial Intelligence” Project designed the first competency-based applied artificial intelligence curriculum at the higher-education institution level. The development was based on advanced system research on existing artificial intelligence-related resources and surveying target groups of teachers, information technology students, and employers, which should enhance the performance of implementing artificial intelligence education. A review of applied artificial intelligence was prepared in the form of keyword clustering. The initial data were collected with the help of surveying by identifying job offers, existing artificial intelligence training courses, scientific projects, and real cases. A synthetic analysis of the textual information from the studies was conducted using the word clouds technique. A tensor-based approach was used for the presentation of the competency-based course. The specific numerical requirements for the course in the form of priorities followed from the solution to decision-making problems using the analytic hierarchy process technique. Based on a comprehensive study of surveys, educational experience, scientific projects, and business requirements, and a meta-analysis of the recent references, we specified the criteria for a training course in the form of a tensor-based representation of competencies in relation to content and educational modules. Full article
12 pages, 11813 KiB  
Article
Preparation and Investigation of High Surface Area Aerogels from Crosslinked Polypropylenes
by Radek Coufal, Mateusz Fijalkowski, Kinga Adach, Huaitian Bu, Christian W. Karl, Eliška Mikysková and Stanislav Petrík
Polymers 2024, 16(10), 1382; https://doi.org/10.3390/polym16101382 (registering DOI) - 12 May 2024
Abstract
Polypropylene-based aerogels with high surface area have been developed for the first time. By chemical crosslinking of polypropylene with oligomeric capped-end amino compounds, followed by dissolution, thermally induced phase separation, and the supercritical CO2 drying process or freeze-drying method, the aerogels exhibit [...] Read more.
Polypropylene-based aerogels with high surface area have been developed for the first time. By chemical crosslinking of polypropylene with oligomeric capped-end amino compounds, followed by dissolution, thermally induced phase separation, and the supercritical CO2 drying process or freeze-drying method, the aerogels exhibit high specific surface areas up to 200 m2/g. Moreover, the silica-cage multi-amino compound was utilized in a similar vein for forming hybrid polypropylene aerogels. According to the SEM, the developed polypropylene-based aerogels exhibit highly porous morphology with micro-nanoscale structural features that can be controlled by processing conditions. Our simple and inexpensive synthetic strategy results in a low-cost, chemically resistant, and highly porous material that can be tailored according to end-use applications. Full article
(This article belongs to the Section Polymer Chemistry)
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23 pages, 2306 KiB  
Article
Study on the Fracture of a Shield Segment in a Fully Excavated Hard Rock Section under the Influence of Construction Loads
by Cheng Zhu, Bin Zheng, Guoping Ren, Tugen Feng, Xiaochun Zhong and Siyuan Huang
Appl. Sci. 2024, 14(10), 4102; https://doi.org/10.3390/app14104102 (registering DOI) - 12 May 2024
Abstract
In this paper, the initiation of the fracture of a segment caused by the pressure of the jack and other factors during shield construction is discussed. Based on the Rots model in the finite element software Diana 10.4 , the fracture width is [...] Read more.
In this paper, the initiation of the fracture of a segment caused by the pressure of the jack and other factors during shield construction is discussed. Based on the Rots model in the finite element software Diana 10.4 , the fracture width is solved. Combined with in situ measurements, the mechanisms of concrete fracturing of a segment under external loads, such as the jack thrust deflection angle and uneven jack thrust caused by the changes in the segment due to the upward buoyancy and shield attitude, are studied; additionally, the occurrence conditions and engineering control measures for segment fracture are summarized. The results show that when the attitudes of the shield and segment are identical, the total thrust of the shield is recommended not to exceed 21,000 kN, and is strictly limited to 24,000 kN. When the attitude inclination angle between the shield machine and the segment is less than 1°, the impact on the segment quality is small. When the inclination angle reaches 2°, the total thrust of the shield is recommended not to exceed 16,000 kN, and is strictly limited to 18,000 kN. When the inclination reaches 3°, a fracture is easily produced. When the total thrust is 19,000 kN, it is recommended that the loading increase or decrease in the left and right four grippers should not exceed 20%, and they are prohibited to exceed 30%. The fracture width increases exponentially with the increase in misalignment between adjacent segment rings. These research results provide a theoretical basis for jack pressure control during shield construction. Full article
12 pages, 1756 KiB  
Article
Evaluation of Serial Procalcitonin Levels for the Optimization of Antibiotic Use in Non-Critically Ill COVID-19 Patients
by Abdulaziz S. Almulhim, Mohammed A. Alabdulwahed, Fatimah F. Aldoughan, Ali M. Aldayyen, Faisal Alghamdi, Rawan Alabdulqader, Norah Alnaim, Dimah Alghannam, Yasmin Aljamaan, Saleh Almutairi, Feras T. Al Mogbel, Ahmad Alamer and Haytham A. Wali
Pharmaceuticals 2024, 17(5), 624; https://doi.org/10.3390/ph17050624 (registering DOI) - 12 May 2024
Abstract
Background: Procalcitonin (PCT) has been used as a biomarker to guide antibiotic therapy in various patient populations. However, its role in optimizing antibiotic use in COVID-19 patients has not been well studied to date. Thus, we aimed to evaluate the use of serial [...] Read more.
Background: Procalcitonin (PCT) has been used as a biomarker to guide antibiotic therapy in various patient populations. However, its role in optimizing antibiotic use in COVID-19 patients has not been well studied to date. Thus, we aimed to evaluate the use of serial PCT monitoring as an antimicrobial stewardship tool for COVID-19 patients. Methods: This retrospective study included 240 COVID-19 patients who were admitted to a tertiary medical institution in Saudi Arabia between January 2020 and February 2022. Patients who received empiric antibiotic therapy for community-acquired pneumonia (CAP) and had serial procalcitonin levels were included. The patients were divided into two groups: the normal procalcitonin arm (PCT level < 0.5 ng/mL) and the elevated PCT arm (PCT level > 0.5 ng/mL). The primary and secondary outcomes were the effect of PCT monitoring on the duration of antibiotic exposure and the length of hospital stay, respectively. To measure the accuracy of PCT, the receiver-operating characteristic area under the curve (ROC-AUC) was determined. Results: Among the included patients, 142 were in the normal procalcitonin arm (median PCT, 0.12 ng/mL), and 78 were in the elevated PCT arm (median PCT, 4.04 ng/mL). The baseline characteristics were similar between the two arms, except for the higher prevalence of kidney disease in the elevated PCT arm. There was no statistically significant difference in the duration of antibiotic exposure between the normal and elevated PCT arms (median duration: 7 days in both arms). However, the length of hospital stay was significantly shorter in the normal PCT arm (median stay, 9 days) than in the elevated PCT arm (median stay, 13 days; p = 0.028). The ROC-AUC value was 0.54 (95% CI: 0.503–0.595). Conclusions: Serial PCT monitoring did not lead to a reduction in the duration of antibiotic exposure in COVID-19 patients. However, it was associated with a shorter hospital stay. These findings suggest that PCT monitoring may be useful for optimizing antibiotic use and improving outcomes in COVID-19 patients. While PCT-guided algorithms have the potential to enable antibiotic stewardship, their role in the context of COVID-19 treatment requires further investigation. Full article
(This article belongs to the Special Issue Infectious Disease Epidemiology and Pharmaceutical Development)
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22 pages, 7376 KiB  
Article
Can Plants Perceive Human Gestures? Using AI to Track Eurythmic Human–Plant Interaction
by Alvaro Francisco Gil, Moritz Weinbeer and Peter A. Gloor
Biomimetics 2024, 9(5), 290; https://doi.org/10.3390/biomimetics9050290 (registering DOI) - 12 May 2024
Abstract
This paper explores if plants are capable of responding to human movement by changes in their electrical signals. Toward that goal, we conducted a series of experiments, where humans over a period of 6 months were performing different types of eurythmic gestures in [...] Read more.
This paper explores if plants are capable of responding to human movement by changes in their electrical signals. Toward that goal, we conducted a series of experiments, where humans over a period of 6 months were performing different types of eurythmic gestures in the proximity of garden plants, namely salad, basil, and tomatoes. To measure plant perception, we used the plant SpikerBox, which is a device that measures changes in the voltage differentials of plants between roots and leaves. Using machine learning, we found that the voltage differentials over time of the plant predict if (a) eurythmy has been performed, and (b) which kind of eurythmy gestures has been performed. We also find that the signals are different based on the species of the plant. In other words, the perception of a salad, tomato, or basil might differ just as perception of different species of animals differ. This opens new ways of studying plant ecosystems while also paving the way to use plants as biosensors for analyzing human movement. Full article
(This article belongs to the Special Issue Biomimetics in Intelligent Sensor)
13 pages, 519 KiB  
Article
The Relationship between Coping Strategies and State Anxiety during COVID-19 Lockdown: The Role of Perceived Emotional Intelligence
by Alessandro Geraci, Laura Di Domenico and Antonella D’Amico
COVID 2024, 4(5), 592-604; https://doi.org/10.3390/covid4050040 (registering DOI) - 12 May 2024
Abstract
Background: The outbreak of the COVID-19 pandemic has caused an unprecedented and unexpected change all around the globe. The long-term effects are still ongoing, especially those related to the confinement measures. The study took place during the first COVID-19 lockdown in Italy, where [...] Read more.
Background: The outbreak of the COVID-19 pandemic has caused an unprecedented and unexpected change all around the globe. The long-term effects are still ongoing, especially those related to the confinement measures. The study took place during the first COVID-19 lockdown in Italy, where everyone was forced to stay home in order to reduce the spread of the virus. The aim was to investigate the role of perceived emotional intelligence abilities (PEI) in coping with COVID-19-related anxiety. Methods: A cross-sectional study design was employed, and this study used an online survey launched through social networks, inviting adults to participate. The participants anonymously completed a three-scale online measurement of self-reported emotional abilities, coping strategies (approach and avoidance), and state anxiety towards COVID-19. Results: perceived emotional intelligence and approach coping significantly predicted state anxiety. In addition, perceived emotional intelligence mediated the relationship between approach coping and state anxiety. Conclusions: the study highlights the positive role of perceived emotional abilities in dealing with the unprecedented event represented by the COVID-19 pandemic, and in particular, in coping with anxiety related to lockdown and confinement. Their results highlight the importance of fostering emotional intelligence for navigating critical life events. Full article
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