The 2023 MDPI Annual Report has
been released!
 
24 pages, 4849 KiB  
Article
Amyloid Beta Leads to Decreased Acetylcholine Levels and Non-Small Cell Lung Cancer Cell Survival via a Mechanism That Involves p38 Mitogen-Activated Protein Kinase and Protein Kinase C in a p53-Dependent and -Independent Manner
by Hind Al Khashali, Ravel Ray, Ban Darweesh, Caroline Wozniak, Ben Haddad, Stuti Goel, Issah Seidu, Jeneen Khalil, Brooke Lopo, Nayrooz Murshed, Jeffrey Guthrie, Deborah Heyl and Hedeel Guy Evans
Int. J. Mol. Sci. 2024, 25(9), 5033; https://doi.org/10.3390/ijms25095033 (registering DOI) - 05 May 2024
Abstract
Several studies have shown an inverse correlation between the likelihood of developing a neurodegenerative disorder and cancer. We previously reported that the levels of amyloid beta (Aβ), at the center of Alzheimer’s disease pathophysiology, are regulated by acetylcholinesterase (AChE) in non-small cell lung [...] Read more.
Several studies have shown an inverse correlation between the likelihood of developing a neurodegenerative disorder and cancer. We previously reported that the levels of amyloid beta (Aβ), at the center of Alzheimer’s disease pathophysiology, are regulated by acetylcholinesterase (AChE) in non-small cell lung cancer (NSCLC). Here, we examined the effect of Aβ or its fragments on the levels of ACh in A549 (p53 wild-type) and H1299 (p53-null) NSCLC cell media. ACh levels were reduced by cell treatment with Aβ 1–42, Aβ 1–40, Aβ 1–28, and Aβ 25–35. AChE and p53 activities increased upon A549 cell treatment with Aβ, while knockdown of p53 in A549 cells increased ACh levels, decreased AChE activity, and diminished the Aβ effects. Aβ increased the ratio of phospho/total p38 MAPK and decreased the activity of PKC. Inhibiting p38 MAPK reduced the activity of p53 in A549 cells and increased ACh levels in the media of both cell lines, while opposite effects were found upon inhibiting PKC. ACh decreased the activity of p53 in A549 cells, decreased p38 MAPK activity, increased PKC activity, and diminished the effect of Aβ on those activities. Moreover, the negative effect of Aβ on cell viability was diminished by cell co-treatment with ACh. Full article
(This article belongs to the Special Issue Role of p53 Family in Targeted Therapy of Cancers)
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22 pages, 1327 KiB  
Article
Can Creating an Agro-Product Regional Public Brand Improve the Ability of Farmers to Sustainably Increase Their Revenue?
by Zhiyan Ma and Guanghua Qiao
Sustainability 2024, 16(9), 3861; https://doi.org/10.3390/su16093861 (registering DOI) - 05 May 2024
Abstract
Through the analysis of various typical cases, this study examines whether the establishment of an agro-product regional public brand (ARPB) can effectively boost the revenue of farmers and increase their share in the supply chain. The findings suggest that an early-stage ARPB can [...] Read more.
Through the analysis of various typical cases, this study examines whether the establishment of an agro-product regional public brand (ARPB) can effectively boost the revenue of farmers and increase their share in the supply chain. The findings suggest that an early-stage ARPB can command a price premium for products, yet its overall contribution to farmers’ revenue remains limited due to scale constraints. The premium ability of an ARPB is influenced by product characteristics and sales strategies, underscoring the need to enhance control over terminal sales. Although the revenue of all operators in an ARPB supply chain shows an increase compared to that of a non-ARPB supply chain, the ratios of revenue allocated to farmers diminish. The Shapley value method was utilized to optimize the revenue-sharing in the supply chain, indicating a need to increase the share of revenue for farmers. This optimization necessitates the formation of a community of interests between farmers, processing enterprises, and sellers to facilitate the upstream movement of brand premiums. Furthermore, enhancing the government’s mediation and regulatory functions can provide farmers with more opportunities to partake in brand benefits. Full article
(This article belongs to the Section Sustainable Agriculture)
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16 pages, 869 KiB  
Article
Understanding Elderly Chinese Cancer Patients in a Multicultural Clinical Setting: Embracing Mortality and Addressing Misperceptions of Vulnerability
by Yvonne W. Leung, Enid W. Y. Kwong, Karen Lok Yi Wong, Jeremiah So, Frankie Poon, Terry Cheng, Eric Chen, Alex Molasiotis and Doris Howell
Curr. Oncol. 2024, 31(5), 2620-2635; https://doi.org/10.3390/curroncol31050197 (registering DOI) - 05 May 2024
Abstract
Chinese patients face higher risks of gastrointestinal (GI) cancers and greater cancer-related deaths than Canadian-born patients. The older population encounters barriers to quality healthcare, impacting their well-being and survival. Previous studies highlighted Chinese immigrant perceptions of not requiring healthcare support. During the COVID-19 [...] Read more.
Chinese patients face higher risks of gastrointestinal (GI) cancers and greater cancer-related deaths than Canadian-born patients. The older population encounters barriers to quality healthcare, impacting their well-being and survival. Previous studies highlighted Chinese immigrant perceptions of not requiring healthcare support. During the COVID-19 pandemic, their underutilization of healthcare services garnered attention. The present study explores the experiences of older Chinese cancer patients to improve culturally sensitive cancer care. A total of twenty interviews carried out in Cantonese and Mandarin were conducted with Chinese immigrants, aged 60 or above, diagnosed with Stage 3 or 4 GI cancer. These interviews were transcribed verbatim, translated, and subjected to qualitative descriptive analysis. Among older Chinese immigrant patients, a phenomenon termed “Premature Acceptance: Normalizing Death and Dying” was observed. This involved four key themes: 1. acceptance and letting go, 2. family first, 3. self-sufficiency, and 4. barriers to supportive care. Participants displayed an early acceptance of their own mortality, prioritizing family prosperity over their own quality of life. Older Chinese patients normalize the reality of facing death amidst cancer. They adopt a pragmatic outlook, acknowledging life-saving treatments while willingly sacrificing their own support needs to ease family burdens. Efforts to enhance health literacy require culturally sensitive programs tailored to address language barriers and differing values among this population. A strengths-based approach emphasizing family support and practical aspects of care may help build resilience and improve symptom management, thereby enhancing their engagement with healthcare services. Full article
(This article belongs to the Topic From Basic Research to a Clinical Perspective in Oncology)
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42 pages, 6682 KiB  
Article
Automated Porosity Characterization for Aluminum Die Casting Materials Using X-ray Radiography, Synthetic X-ray Data Augmentation by Simulation, and Machine Learning
by Stefan Bosse, Dirk Lehmhus and Sanjeev Kumar
Sensors 2024, 24(9), 2933; https://doi.org/10.3390/s24092933 (registering DOI) - 05 May 2024
Abstract
Detection and characterization of hidden defects, impurities, and damages in homogeneous materials like aluminum die casting materials, as well as composite materials like Fiber–Metal Laminates (FML), is still a challenge. This work discusses methods and challenges in data-driven modeling of automated damage and [...] Read more.
Detection and characterization of hidden defects, impurities, and damages in homogeneous materials like aluminum die casting materials, as well as composite materials like Fiber–Metal Laminates (FML), is still a challenge. This work discusses methods and challenges in data-driven modeling of automated damage and defect detectors using measured X-ray single- and multi-projection images. Three main issues are identified: Data and feature variance, data feature labeling (for supervised machine learning), and the missing ground truth. It will be shown that simulation of synthetic measuring data can deliver a ground truth dataset and accurate labeling for data-driven modeling, but it cannot be used directly to predict defects in manufacturing processes. Noise has a significant impact on the feature detection and will be discussed. Data-driven feature detectors are implemented with semantic pixel Convolutional Neural Networks. Experimental data are measured with different devices: A low-quality and low-cost (Low-Q) X-ray radiography, a typical industrial mid-quality X-ray radiography and Computed Tomography (CT) system, and a state-of-the-art high-quality μ-CT device. The goals of this work are the training of robust and generalized data-driven ML feature detectors with synthetic data only and the transition from CT to single-projection radiography imaging and analysis. Although, as the title implies, the primary task is pore characterization in aluminum high-pressure die-cast materials, but the methods and results are not limited to this use case. Full article
(This article belongs to the Section Sensing and Imaging)
19 pages, 1448 KiB  
Review
Short-Chained Linear Scorpion Peptides: A Pool for Novel Antimicrobials
by Tolis Panayi, Spiridoula Diavoli, Vicky Nicolaidou, Christos Papaneophytou, Christos Petrou and Yiannis Sarigiannis
Antibiotics 2024, 13(5), 422; https://doi.org/10.3390/antibiotics13050422 (registering DOI) - 05 May 2024
Abstract
Scorpion venom peptides are generally classified into two main groups: the disulfide bridged peptides (DBPs), which usually target membrane-associated ion channels, and the non-disulfide bridged peptides (NDBPs), a smaller group with multifunctional properties. In the past decade, these peptides have gained interest because [...] Read more.
Scorpion venom peptides are generally classified into two main groups: the disulfide bridged peptides (DBPs), which usually target membrane-associated ion channels, and the non-disulfide bridged peptides (NDBPs), a smaller group with multifunctional properties. In the past decade, these peptides have gained interest because most of them display functions that include antimicrobial, anticancer, haemolytic, and anti-inflammatory activities. Our current study focuses on the short (9–19 amino acids) antimicrobial linear scorpion peptides. Most of these peptides display a net positive charge of 1 or 2, an isoelectric point at pH 9–10, a broad range of hydrophobicity, and a Grand Average of Hydropathy (GRAVY) Value ranging between −0.05 and 1.7. These features allow these peptides to be attracted toward the negatively charged phospholipid head groups of the lipid membranes of target cells, a force driven by electrostatic interactions. This review outlines the antimicrobial potential of short-chained linear scorpion venom peptides. Additionally, short linear scorpion peptides are in general more attractive for large-scale synthesis from a manufacturing point of view. The structural and functional diversity of these peptides represents a good starting point for the development of new peptide-based therapeutics. Full article
(This article belongs to the Special Issue Insights into Natural Antimicrobial Peptides)
21 pages, 1029 KiB  
Review
A Review of the CACNA Gene Family: Its Role in Neurological Disorders
by Oliwia Szymanowicz, Artur Drużdż, Bartosz Słowikowski, Sandra Pawlak, Ewelina Potocka, Ulyana Goutor, Mateusz Konieczny, Małgorzata Ciastoń, Aleksandra Lewandowska, Paweł P. Jagodziński, Wojciech Kozubski and Jolanta Dorszewska
Diseases 2024, 12(5), 90; https://doi.org/10.3390/diseases12050090 (registering DOI) - 05 May 2024
Abstract
Calcium channels are specialized ion channels exhibiting selective permeability to calcium ions. Calcium channels, comprising voltage-dependent and ligand-gated types, are pivotal in neuronal function, with their dysregulation is implicated in various neurological disorders. This review delves into the significance of the CACNA genes, [...] Read more.
Calcium channels are specialized ion channels exhibiting selective permeability to calcium ions. Calcium channels, comprising voltage-dependent and ligand-gated types, are pivotal in neuronal function, with their dysregulation is implicated in various neurological disorders. This review delves into the significance of the CACNA genes, including CACNA1A, CACNA1B, CACNA1C, CACNA1D, CACNA1E, CACNA1G, and CACNA1H, in the pathogenesis of conditions such as migraine, epilepsy, cerebellar ataxia, dystonia, and cerebellar atrophy. Specifically, variants in CACNA1A have been linked to familial hemiplegic migraine and epileptic seizures, underscoring its importance in neurological disease etiology. Furthermore, different genetic variants of CACNA1B have been associated with migraine susceptibility, further highlighting the role of CACNA genes in migraine pathology. The complex relationship between CACNA gene variants and neurological phenotypes, including focal seizures and ataxia, presents a variety of clinical manifestations of impaired calcium channel function. The aim of this article was to explore the role of CACNA genes in various neurological disorders, elucidating their significance in conditions such as migraine, epilepsy, and cerebellar ataxias. Further exploration of CACNA gene variants and their interactions with molecular factors, such as microRNAs, holds promise for advancing our understanding of genetic neurological disorders. Full article
(This article belongs to the Section Rare Syndrome)
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12 pages, 408 KiB  
Article
An Online-Based Survey to Assess Knowledge, Attitudes, and Barriers to Perform First Aid after Road Accidents Conducted among Adult Jordanians
by Walid Al-Qerem, Anan Jarab, Abdel Qader Al Bawab, Alaa Hammad, Judith Eberhardt, Fawaz Alasmari, Haneen Kalloush, Lujain Al-Sa’di and Raghd Obidat
Healthcare 2024, 12(9), 947; https://doi.org/10.3390/healthcare12090947 (registering DOI) - 05 May 2024
Abstract
(1) Background: First aid administered during road accidents can save millions of lives. However, the knowledge and attitudes of the Jordanian population towards first aid are lacking. This study aimed to examine the knowledge, attitudes, and barriers to performing first aid among the [...] Read more.
(1) Background: First aid administered during road accidents can save millions of lives. However, the knowledge and attitudes of the Jordanian population towards first aid are lacking. This study aimed to examine the knowledge, attitudes, and barriers to performing first aid among the Jordanian population during road accidents. (2) Methods: An online questionnaire was developed and distributed using various Jordanian social media platforms. The questionnaire collected the participants’ sociodemographic details and assessed their first aid knowledge, attitudes toward first aid, and barriers preventing the participants from performing first aid in emergencies. (3) Results: 732 participants participated in this study. The median knowledge score regarding first aid items was 9 (7–10) out of the maximum possible score of 15. The median first aid attitude score was 24 (22–27) out of a maximum possible score of 30. The most commonly reported barrier to performing first aid among the participants was “lack of first aid training” (76.78%), followed by “lack of knowledge about first aid” (75.81%) and “fear of performing first aid” (57.51%). The participants with lower income levels exhibited more negative attitudes towards first aid (4). Conclusions: This study underscores the urgent need for enhanced first aid training and awareness in Jordan. The participants’ first-aid knowledge overall was limited, although positive attitudes toward first-aid delivery were observed. The findings emphasize the need for regular and structured first-aid training courses, addressing barriers such as fear and misinformation and ensuring accessibility across all socioeconomic levels to improve preparedness for road traffic accidents and other emergencies. This comprehensive approach can better equip the Jordanian population to effectively manage emergencies and improve public health outcomes. Full article
14 pages, 2444 KiB  
Article
Environmental Impact Assessment of Solid Oxide Fuel Cell Power Generation System Based on Life Cycle Assessment—A Case Study in China
by Yilin Shen, Yantao Yang, Lei Song and Tingzhou Lei
Sustainability 2024, 16(9), 3863; https://doi.org/10.3390/su16093863 (registering DOI) - 05 May 2024
Abstract
To progress towards the “dual carbon” goal and reduce the cost and increase the efficiency of solid oxide fuel cells, this study conducts a full life cycle analysis of solid oxide fuel cells, in which the environmental impact caused by the operating devices’ [...] Read more.
To progress towards the “dual carbon” goal and reduce the cost and increase the efficiency of solid oxide fuel cells, this study conducts a full life cycle analysis of solid oxide fuel cells, in which the environmental impact caused by the operating devices’ manufacturing, fuel gas catalyst reforming, single-cell manufacturing, cell stack manufacturing, and energy consumption and emissions are systematically analysed. In this study, we establish an assessment model for solid oxide fuel cells by using the cut-off criterion. The results show that 96.5% of the global warming potential in the use of solid oxide fuel cells comes from the stack operating subsystem. The stack manufacturing subsystem, operating device manufacturing subsystem, and waste stack processing subsystem all contribute greatly to acidification, accounting for 32.89%, 44%, and 35.82% of the total acidification, respectively. These three subsystems also contribute significantly to eutrophication, contributing 23.11%, 22.03%, and 42.15%, respectively. Compared with traditional thermal power generation systems, solid oxide fuel cell power generation systems have slightly higher overall environmental benefits, and the reductions in greenhouse gas emissions and acidification potential reach 6.22% and 18.52%, respectively. The research results have guiding significance and reference value for subsequent energy-saving and emission reduction design and improvement efforts for solid oxide fuel cells. Full article
(This article belongs to the Collection Environmental Assessment, Life Cycle Analysis and Sustainability)
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17 pages, 3218 KiB  
Review
Opportunities and Challenges of Soy Proteins with Different Processing Applications
by Zixiao Deng and Sung Woo Kim
Antioxidants 2024, 13(5), 569; https://doi.org/10.3390/antiox13050569 (registering DOI) - 05 May 2024
Abstract
Soybean meal (SBM) is a prevailing plant protein supplement in animal diets because of its nutritional value and availability. This review paper explores the significance of SBM and processed soy products, emphasizing their nutritional and bioactive components, such as isoflavones and soyasaponins. These [...] Read more.
Soybean meal (SBM) is a prevailing plant protein supplement in animal diets because of its nutritional value and availability. This review paper explores the significance of SBM and processed soy products, emphasizing their nutritional and bioactive components, such as isoflavones and soyasaponins. These compounds are known for their antioxidant and anti-inflammatory properties and are associated with a reduced prevalence of chronic diseases. However, the presence of antinutritional compounds in SBM presents a significant challenge. The paper evaluates various processing methods, including ethanol/acid wash, enzyme treatment, and fermentation, which are aimed at enhancing the nutritional value of soy products. It highlights the significance to maintain a balance between nutritional enhancement and the preservation of beneficial bioactive compounds, emphasizing the importance of different processing techniques to fully exploit the health benefits of soy-based products. Therefore, this review illuminates the complex balance between nutritional improvement, bioactive compound preservation, and the overall health implications of soy products. Full article
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12 pages, 1201 KiB  
Brief Report
Rapid Detection and Quick Characterization of African Swine Fever Virus Using the VolTRAX Automated Library Preparation Platform
by Vivian O’Donnell, Jim L. Pierce, Oleg Osipenko, Lizhe Xu, Amy Berninger, Steven M. Lakin, Roger W. Barrette, Douglas P. Gladue and Bonto Faburay
Viruses 2024, 16(5), 731; https://doi.org/10.3390/v16050731 (registering DOI) - 05 May 2024
Abstract
African swine fever virus (ASFV) is the causative agent of a severe and highly contagious viral disease affecting domestic and wild swine. The current ASFV pandemic strain has a high mortality rate, severely impacting pig production and, for countries suffering outbreaks, preventing the [...] Read more.
African swine fever virus (ASFV) is the causative agent of a severe and highly contagious viral disease affecting domestic and wild swine. The current ASFV pandemic strain has a high mortality rate, severely impacting pig production and, for countries suffering outbreaks, preventing the export of their pig products for international trade. Early detection and diagnosis of ASFV is necessary to control new outbreaks before the disease spreads rapidly. One of the rate-limiting steps to identify ASFV by next-generation sequencing platforms is library preparation. Here, we investigated the capability of the Oxford Nanopore Technologies’ VolTRAX platform for automated DNA library preparation with downstream sequencing on Nanopore sequencing platforms as a proof-of-concept study to rapidly identify the strain of ASFV. Within minutes, DNA libraries prepared using VolTRAX generated near-full genome sequences of ASFV. Thus, our data highlight the use of the VolTRAX as a platform for automated library preparation, coupled with sequencing on the MinION Mk1C for field sequencing or GridION within a laboratory setting. These results suggest a proof-of-concept study that VolTRAX is an effective tool for library preparation that can be used for the rapid and real-time detection of ASFV. Full article
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15 pages, 627 KiB  
Article
Investigation of Liver X Receptor Gene Variants and Oxysterol Dysregulation in Autism Spectrum Disorder
by Tuğba Menteşe Babayiğit, Güvem Gümüş-Akay, Merve Çikili Uytun, Özlem Doğan, Muhittin A. Serdar, Gökçe Yağmur Efendi, Ayşe Gökçe Erman, Esra Yürümez and Didem Behice Öztop
Children 2024, 11(5), 551; https://doi.org/10.3390/children11050551 (registering DOI) - 05 May 2024
Abstract
The NR1H2 gene produces the Liver X Receptor Beta (LXRB) protein, which is crucial for brain cholesterol metabolism and neuronal development. However, its involvement in autism spectrum disorder (ASD) remains largely unexplored, aside from animal studies. This study is the first to explore [...] Read more.
The NR1H2 gene produces the Liver X Receptor Beta (LXRB) protein, which is crucial for brain cholesterol metabolism and neuronal development. However, its involvement in autism spectrum disorder (ASD) remains largely unexplored, aside from animal studies. This study is the first to explore the potential link between autism and rs2695121/rs17373080 single nucleotide polymorphisms (SNPs) in the regulatory regions of NR1H2, known for their association with neuropsychiatric functions. Additionally, we assessed levels of oxysterols (24-Hydroxycholesterol, 25-Hydroxycholesterol, 27-Hydroxycholesterol), crucial ligands of LXR, and lipid profiles. Our cohort comprised 107 children with ASD and 103 healthy children aged 2–18 years. Clinical assessment tools included the Childhood Autism Rating Scale, Autistic Behavior Checklist, and Repetitive Behavior Scale-Revised. Genotyping for SNPs was conducted using PCR-RFLP. Lipid profiles were analyzed with Beckman Coulter kits, while oxysterol levels were determined through liquid chromatography–tandem mass spectrometry. Significantly higher total cholesterol (p = 0.003), LDL (p = 0.008), and triglyceride (p < 0.001) levels were observed in the ASD group. 27-Hydroxycholesterol levels were markedly lower in the ASD group (p ≤ 0.001). ROC analysis indicated the potential of 27-Hydroxycholesterol to discriminate ASD diagnosis. The SNP genotype and allele frequencies were similar in both groups (p > 0.05). Our findings suggest that disturbances in oxysterol metabolism, previously linked to neurodegeneration, may constitute a risk factor for ASD and contribute to its heterogeneous phenotype. Full article
(This article belongs to the Section Child and Adolescent Psychiatry)
18 pages, 13276 KiB  
Article
Microstructural Investigation of Process Parameters Dedicated to Laser Powder Bed Fusion of AlSi7Mg0.6 Alloy
by Janusz Kluczyński, Tomáš Dražan, Zdeněk Joska, Jakub Łuszczek, Robert Kosturek and Katarzyna Jasik
Materials 2024, 17(9), 2156; https://doi.org/10.3390/ma17092156 (registering DOI) - 05 May 2024
Abstract
This study presents a microstructural investigation of the printing parameters of an AlSi7Mg0.6 alloy produced by powder bed fusion (PBF) using laser beam melting (LB/M) technology. The investigation focused on the effects of laser power, exposure velocity, and hatching distance on the microhardness, [...] Read more.
This study presents a microstructural investigation of the printing parameters of an AlSi7Mg0.6 alloy produced by powder bed fusion (PBF) using laser beam melting (LB/M) technology. The investigation focused on the effects of laser power, exposure velocity, and hatching distance on the microhardness, porosity, and microstructure of the produced alloy. The microstructure was characterized in the plane of printing on a confocal microscope. The results showed that the printing parameters significantly affected the microstructure, whereas the energy density had a major effect. Decreasing the laser power and decreasing the hatching distance resulted in increased porosity and the increased participation of non-melted particles. A mathematical model was created to determine the porosity of a 3D-printed material based on three printing parameters. Microhardness was not affected by the printing parameters. The statistical model created based on the porosity investigation allowed for the illustration of the technological window and showed certain ranges of parameter values at which the porosity of the produced samples was at a possible low level. Full article
(This article belongs to the Special Issue Laser Technology for Materials Processing)
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19 pages, 2594 KiB  
Review
Advanced Home-Based Shoulder Rehabilitation: A Systematic Review of Remote Monitoring Devices and Their Therapeutic Efficacy
by Martina Sassi, Mariajose Villa Corta, Matteo Giuseppe Pisani, Guido Nicodemi, Emiliano Schena, Leandro Pecchia and Umile Giuseppe Longo
Sensors 2024, 24(9), 2936; https://doi.org/10.3390/s24092936 (registering DOI) - 05 May 2024
Abstract
Shoulder pain represents the most frequently reported musculoskeletal disorder, often leading to significant functional impairment and pain, impacting quality of life. Home-based rehabilitation programs offer a more accessible and convenient solution for an effective shoulder disorder treatment, addressing logistical and financial constraints associated [...] Read more.
Shoulder pain represents the most frequently reported musculoskeletal disorder, often leading to significant functional impairment and pain, impacting quality of life. Home-based rehabilitation programs offer a more accessible and convenient solution for an effective shoulder disorder treatment, addressing logistical and financial constraints associated with traditional physiotherapy. The aim of this systematic review is to report the monitoring devices currently proposed and tested for shoulder rehabilitation in home settings. The research question was formulated using the PICO approach, and the PRISMA guidelines were applied to ensure a transparent methodology for the systematic review process. A comprehensive search of PubMed and Scopus was conducted, and the results were included from 2014 up to 2023. Three different tools (i.e., the Rob 2 version of the Cochrane risk-of-bias tool, the Joanna Briggs Institute (JBI) Critical Appraisal tool, and the ROBINS-I tool) were used to assess the risk of bias. Fifteen studies were included as they fulfilled the inclusion criteria. The results showed that wearable systems represent a promising solution as remote monitoring technologies, offering quantitative and clinically meaningful insights into the progress of individuals within a rehabilitation pathway. Recent trends indicate a growing use of low-cost, non-intrusive visual tracking devices, such as camera-based monitoring systems, within the domain of tele-rehabilitation. The integration of home-based monitoring devices alongside traditional rehabilitation methods is acquiring significant attention, offering broader access to high-quality care, and potentially reducing healthcare costs associated with in-person therapy. Full article
(This article belongs to the Special Issue Intelligent Sensors for Healthcare and Patient Monitoring)
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12 pages, 668 KiB  
Article
Emerging and Reemerging Parasitic Diseases in Taiwan: A Retrospective Study of Clinical Case Reports in 2001~2018
by Shao-Lun Hsu and Chia-Kwung Fan
Pathogens 2024, 13(5), 383; https://doi.org/10.3390/pathogens13050383 (registering DOI) - 05 May 2024
Abstract
Emerging and re-emerging parasitic diseases can cause significant economic burdens at national and global levels. However, governments often underestimate or ignore these diseases, especially in developed countries. This retrospective, case-oriented study analyzed parasitic diseases reported in Taiwan between 2001 and 2018. One hundred [...] Read more.
Emerging and re-emerging parasitic diseases can cause significant economic burdens at national and global levels. However, governments often underestimate or ignore these diseases, especially in developed countries. This retrospective, case-oriented study analyzed parasitic diseases reported in Taiwan between 2001 and 2018. One hundred and thirty-two eligible clinical profiles of Taiwanese patients obtained from the NCBI, Scopus, Google Scholar, and Web of Science databases and local journals according to age, sex, source of infection, symptoms, risk factors, and geographical regions were analyzed. The analysis results showed that the number/frequency of cases caused by nematodes (46.97%) or protozoa (37.88%) was significantly higher than that of trematodes (9.85%) or cestodes (5.30%) (p < 0.0001). Northern Taiwan (46.97%) had a significantly higher rate than Southern Taiwan (33.33%), Central Taiwan (8.33%), and Eastern Taiwan (5.30%) (p < 0.05). The 15–65 age group (68.94%) had a significantly higher rate than the 65–90 age group (22.73%) and the 0–15 age group (8.33%) (p < 0.0001). Males (70.46%) had a significantly higher number/frequency of cases than females (29.54%) (p < 0.0001). People who acquired the infection through the food/soil route (32.58%) or who had a low immune status (32.58%) had a higher rate than travel-related infections (15.15%) (p < 0.001). The present study showed that emerging/reemerging parasitic infections continue to be of great concern to the lives and health of Taiwanese citizens and, if ignored, will threaten the health of the Taiwanese people; therefore, the delineation of preventive measures by health authorities is urgently warranted. Full article
(This article belongs to the Special Issue Parasite Infection and Tropical Infectious Diseases)
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15 pages, 825 KiB  
Article
An Improved Lightweight Deep Learning Model and Implementation for Track Fastener Defect Detection with Unmanned Aerial Vehicles
by Qi Yu, Ao Liu, Xinxin Yang and Weimin Diao
Electronics 2024, 13(9), 1781; https://doi.org/10.3390/electronics13091781 (registering DOI) - 05 May 2024
Abstract
Track fastener defect detection is an essential component in ensuring railway safety operations. Traditional manual inspection methods no longer meet the requirements of modern railways. The use of deep learning image processing techniques for classifying and recognizing abnormal fasteners is faster, more accurate, [...] Read more.
Track fastener defect detection is an essential component in ensuring railway safety operations. Traditional manual inspection methods no longer meet the requirements of modern railways. The use of deep learning image processing techniques for classifying and recognizing abnormal fasteners is faster, more accurate, and more intelligent. With the widespread use of unmanned aerial vehicles (UAVs), conducting railway inspections using lightweight, low-power devices carried by UAVs has become a future trend. In this paper, we address the characteristics of track fastener detection tasks by improving the YOLOv4-tiny object detection model. We improved the model to output single-scale features and used the K-means++ algorithm to cluster the dataset, obtaining anchor boxes that were better suited to the dataset. Finally, we developed the FPGA platform and deployed the transformed model on this platform. The experimental results demonstrated that the improved model achieved an mAP of 95.1% and a speed of 295.9 FPS on the FPGA, surpassing the performance of existing object detection models. Moreover, the lightweight and low-powered FPGA platform meets the requirements for UAV deployment. Full article
(This article belongs to the Special Issue Innovative Technologies and Services for Unmanned Aerial Vehicles)
14 pages, 448 KiB  
Review
Towards an ITU Standard for DLT Energy Consumption
by Ioannis Nikolaou and Leonidas Anthopoulos
Energies 2024, 17(9), 2222; https://doi.org/10.3390/en17092222 (registering DOI) - 05 May 2024
Abstract
The emergence of Distributed Ledger Technologies (DLT) in the past decade has challenged our imagination to discover new, innovative and disruptive solutions to problems in domains ranging from finance and healthcare to supply chain and Smart Cities. However, the enormous energy consumption that [...] Read more.
The emergence of Distributed Ledger Technologies (DLT) in the past decade has challenged our imagination to discover new, innovative and disruptive solutions to problems in domains ranging from finance and healthcare to supply chain and Smart Cities. However, the enormous energy consumption that has been observed in some of the most successful DLT applications raises the question of their long term sustainability. This article reviews the standardization efforts of the International Telecommunications Union (ITU) to provide guidelines to regulators and policy makers for making informed decisions on the applicability and sustainability of DLT architectures from the point of view of energy consumption. Full article
(This article belongs to the Section B: Energy and Environment)
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18 pages, 3649 KiB  
Article
Interleukin-13 Treatment of Living Lung Tissue Model Alters the Metabolome and Proteome—A Nano-DESI MS Metabolomics and Shotgun Proteomics Study
by Gábor Tóth, Anastasia Golubova, Alexander Falk, Sara Bergström Lind, Mark Nicholas and Ingela Lanekoff
Int. J. Mol. Sci. 2024, 25(9), 5034; https://doi.org/10.3390/ijms25095034 (registering DOI) - 05 May 2024
Abstract
Asthma is a chronic respiratory disease with one of the largest numbers of cases in the world; thus, constant investigation and technical development are needed to unravel the underlying biochemical mechanisms. In this study, we aimed to develop a nano-DESI MS method for [...] Read more.
Asthma is a chronic respiratory disease with one of the largest numbers of cases in the world; thus, constant investigation and technical development are needed to unravel the underlying biochemical mechanisms. In this study, we aimed to develop a nano-DESI MS method for the in vivo characterization of the cellular metabolome. Using air–liquid interface (ALI) cell layers, we studied the role of Interleukin-13 (IL-13) on differentiated lung epithelial cells acting as a lung tissue model. We demonstrate the feasibility of nano-DESI MS for the in vivo monitoring of basal–apical molecular transport, and the subsequent endogenous metabolic response, for the first time. Conserving the integrity of the ALI lung-cell layer enabled us to perform temporally resolved metabolomic characterization followed by “bottom-up” proteomics on the same population of cells. Metabolic remodeling was observed upon histamine and corticosteroid treatment of the IL-13-exposed lung cell monolayers, in correlation with alterations in the proteomic profile. This proof of principle study demonstrates the utility of in vivo nano-DESI MS for characterizing ALI tissue layers, and the new markers identified in our study provide a good starting point for future, larger-scale studies. Full article
(This article belongs to the Special Issue Metabolomics in Health and Disease 2.0)
14 pages, 809 KiB  
Article
Identifying Features of a System of Practice to Inform a Contemporary Competency Framework for Paramedics in Canada
by Alan M. Batt, Meghan Lysko, Jennifer L. Bolster, Pierre Poirier, Derek Cassista, Michael Austin, Cheryl Cameron, Elizabeth A. Donnelly, Becky Donelon, Noël Dunn, William Johnston, Chelsea Lanos, Tyne M. Lunn, Paige Mason, Sean Teed, Charlene Vacon and Walter Tavares
Healthcare 2024, 12(9), 946; https://doi.org/10.3390/healthcare12090946 (registering DOI) - 05 May 2024
Abstract
Introduction: Paramedic practice is highly variable, occurs in diverse contexts, and involves the assessment and management of a range of presentations of varying acuity across the lifespan. As a result, attempts to define paramedic practice have been challenging and incomplete. This has led [...] Read more.
Introduction: Paramedic practice is highly variable, occurs in diverse contexts, and involves the assessment and management of a range of presentations of varying acuity across the lifespan. As a result, attempts to define paramedic practice have been challenging and incomplete. This has led to inaccurate or under-representations of practice that can ultimately affect education, assessment, and the delivery of care. In this study, we outline our efforts to better identify, explore, and represent professional practice when developing a national competency framework for paramedics in Canada. Methods: We used a systems-thinking approach to identify the settings, contexts, features, and influences on paramedic practice in Canada. This approach makes use of the role and influence of system features at the microsystem, mesosystem, exosystem, macrosystem, supra-macrosystem, and chronosystem levels in ways that can provide new insights. We used methods such as rich pictures, diagramming, and systems mapping to explore relationships between these contexts and features. Findings: When we examine the system of practice in paramedicine, multiple layers become evident and within them we start to see details of features that ought to be considered in any future competency development work. Our exploration of the system highlights that paramedic practice considers the person receiving care, caregivers, and paramedics. It involves collaboration within co-located and dispersed teams that are composed of other health and social care professionals, public safety personnel, and others. Practice is enacted across varying geographical, cultural, social, and technical contexts and is subject to multiple levels of policy, regulatory, and legislative influence. Conclusion: Using a systems-thinking approach, we developed a detailed systems map of paramedic practice in Canada. This map can be used to inform the initial stages of a more representative, comprehensive, and contemporary national competency framework for paramedics in Canada. Full article
(This article belongs to the Section Prehospital Care)
27 pages, 2197 KiB  
Review
Promising Application, Efficient Production, and Genetic Basis of Mannosylerythritol Lipids
by Dun Liu, Guanglei Liu and Shiping Liu
Biomolecules 2024, 14(5), 557; https://doi.org/10.3390/biom14050557 (registering DOI) - 05 May 2024
Abstract
Mannosylerythritol lipids (MELs) are a class of glycolipids that have been receiving increasing attention in recent years due to their diverse biological activities. MELs are produced by certain fungi and display a range of bioactivities, making them attractive candidates for various applications in [...] Read more.
Mannosylerythritol lipids (MELs) are a class of glycolipids that have been receiving increasing attention in recent years due to their diverse biological activities. MELs are produced by certain fungi and display a range of bioactivities, making them attractive candidates for various applications in medicine, agriculture, and biotechnology. Despite their remarkable qualities, industrial-scale production of MELs remains a challenge for fungal strains. Excellent fungal strains and fermentation processes are essential for the efficient production of MELs, so efforts have been made to improve the fermentation yield by screening high-yielding strains, optimizing fermentation conditions, and improving product purification processes. The availability of the genome sequence is pivotal for elucidating the genetic basis of fungal MEL biosynthesis. This review aims to shed light on the applications of MELs and provide insights into the genetic basis for efficient MEL production. Additionally, this review offers new perspectives on optimizing MEL production, contributing to the advancement of sustainable biosurfactant technologies. Full article
(This article belongs to the Section Biomacromolecules: Lipids)
17 pages, 876 KiB  
Review
Vitamins in Gynecologic Malignancies
by Natalia Wierzbowska, Tomasz Olszowski, Dariusz Chlubek, Mateusz Kozłowski and Aneta Cymbaluk-Płoska
Nutrients 2024, 16(9), 1392; https://doi.org/10.3390/nu16091392 (registering DOI) - 05 May 2024
Abstract
The combination of vitamin A and D derivatives with classical chemotherapeutic treatments results in more satisfactory outcomes. The use of drug combinations, such as 9cUAB130 with carboplatin and cisplatin with TAC-101, shows enhanced cytotoxic effects and reductions in ovarian tumor volume compared to [...] Read more.
The combination of vitamin A and D derivatives with classical chemotherapeutic treatments results in more satisfactory outcomes. The use of drug combinations, such as 9cUAB130 with carboplatin and cisplatin with TAC-101, shows enhanced cytotoxic effects and reductions in ovarian tumor volume compared to single-drug treatments. Combining cisplatin with calcitriol and progesterone increases VDR expression, potentially enhancing the effectiveness of anticancer therapy in ovarian cancer. The effectiveness of vitamin derivatives in anticancer treatment may vary depending on the characteristics of the tumor and the cell line from which it originated. An increase in thiamine intake of one unit is associated with an 18% decrease in HPV infection. Higher intake of vitamin C by 50 mg/day is linked to a lower risk of cervical neoplasia. Beta-carotene, vitamin C, and vitamin E are associated with risk reductions of 12%, 15%, and 9% in endometrial cancer, respectively. A balanced daily intake of vitamins is important, as both deficiency and excess can influence cancer development. It has been observed that there is a U-shaped relationship between group B vitamins and metabolic markers and clinical outcomes. Full article
(This article belongs to the Special Issue Micronutrients in Women’s Health and Disease)
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19 pages, 7003 KiB  
Article
Arduino-Based Readout Electronics for Nuclear and Particle Physics
by Markus Köhli, Jannis Weimar, Simon Schmidt, Fabian P. Schmidt, Alexander Lambertz, Laura Weber, Jochen Kaminski and Ulrich Schmidt
Sensors 2024, 24(9), 2935; https://doi.org/10.3390/s24092935 (registering DOI) - 05 May 2024
Abstract
Open Hardware-based microcontrollers, especially the Arduino platform, have become a comparably easy-to-use tool for rapid prototyping and implementing creative solutions. Such devices in combination with dedicated front-end electronics can offer low-cost alternatives for student projects, slow control and independently operating small-scale instrumentation. The [...] Read more.
Open Hardware-based microcontrollers, especially the Arduino platform, have become a comparably easy-to-use tool for rapid prototyping and implementing creative solutions. Such devices in combination with dedicated front-end electronics can offer low-cost alternatives for student projects, slow control and independently operating small-scale instrumentation. The capabilities can be extended to data taking and signal analysis at mid-level rates. Two detector realizations are presented, which cover the readouts of proportional counter tubes and of scintillators or wavelength-shifting fibers with silicon photomultipliers (SiPMs). The SiPMTrigger realizes a small-scale design for coincidence readout of SiPMs as a trigger or veto detector. It consists of a custom mixed signal front-end board featuring signal amplification, discrimination and a coincidence unit for rates of up to 200 kHz. The nCatcher transforms an Arduino Nano to a proportional counter readout with pulse shape analysis: time over threshold measurement and a 10-bit analog-to-digital converter for pulse heights. The device is suitable for low-to-medium-rate environments up to 5 kHz, where a good signal-to-noise ratio is crucial. We showcase the monitoring of thermal neutrons. For data taking and slow control, a logger board is presented that features an SD card and GSM/LoRa interface. Full article
(This article belongs to the Special Issue Advances in Particle Detectors and Radiation Detectors)
20 pages, 2347 KiB  
Review
Effect of High-Sucrose Diet on the Occurrence and Progression of Diabetic Retinopathy and Dietary Modification Strategies
by Chen Yang, Yifei Yu and Jianhong An
Nutrients 2024, 16(9), 1393; https://doi.org/10.3390/nu16091393 (registering DOI) - 05 May 2024
Abstract
As the most serious of the many worse new pathological changes caused by diabetes, there are many risk factors for the occurrence and development of diabetic retinopathy (DR). They mainly include hyperglycemia, hypertension, hyperlipidemia and so on. Among them, hyperglycemia is the most [...] Read more.
As the most serious of the many worse new pathological changes caused by diabetes, there are many risk factors for the occurrence and development of diabetic retinopathy (DR). They mainly include hyperglycemia, hypertension, hyperlipidemia and so on. Among them, hyperglycemia is the most critical cause, and plays a vital role in the pathological changes of DR. High-sucrose diets (HSDs) lead to elevated blood glucose levels in vivo, which, through oxidative stress, inflammation, the production of advanced glycation end products (AGEs) and vascular endothelial growth factor (VEGF), cause plenty of pathological damages to the retina and ultimately bring about loss of vision. The existing therapies for DR primarily target the terminal stage of the disease, when irreversible visual impairment has appeared. Therefore, early prevention is particularly critical. The early prevention of DR-related vision loss requires adjustments to dietary habits, mainly by reducing sugar intake. This article primarily discusses the risk factors, pathophysiological processes and molecular mechanisms associated with the development of DR caused by HSDs. It aims to raise awareness of the crucial role of diet in the occurrence and progression of DR, promote timely changes in dietary habits, prevent vision loss and improve the quality of life. The aim is to make people aware of the importance of diet in the occurrence and progression of DR. According to the dietary modification strategies that we give, patients can change their poor eating habits in a timely manner to avoid theoretically avoidable retinopathy and obtain an excellent prognosis. Full article
(This article belongs to the Special Issue Dietary Manipulations: Advances in Metabolism Disease)
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19 pages, 2004 KiB  
Article
Analysis of Atmospheric Boundary Layer Characteristics on Different Underlying Surfaces of the Eastern Tibetan Plateau in Summer
by Xiaohang Wen, Jie Ma and Mei Chen
Remote Sens. 2024, 16(9), 1645; https://doi.org/10.3390/rs16091645 (registering DOI) - 05 May 2024
Abstract
The atmospheric boundary layer is a key region for human activities and the interaction of various layers and is an important channel for the transportation of momentum, heat, and various substances between the free atmosphere and the surface, which has a significant impact [...] Read more.
The atmospheric boundary layer is a key region for human activities and the interaction of various layers and is an important channel for the transportation of momentum, heat, and various substances between the free atmosphere and the surface, which has a significant impact on the development of weather and climate change. During the Second Tibetan Plateau Scientific Expedition and Research Program (STEP) in June 2022, utilizing the comprehensive stereoscopic observation experiment of the “Plateau Low Vortex Network,” this study analyzed the variation characteristics and influencing factors of the atmospheric boundary layer height (ABLH) at three stations with different underlying surface types on the Qinghai–Tibet Plateau (QTP): Qumalai Station (grassland), Southeast Tibet Observation and Research Station for the Alpine Environment (SETORS, forest), and Sieshan Station (cropland). The analysis utilized sounding observation data, microwave radiometer data, and ERA5 reanalysis data. The results revealed that the temperature differences between the sounding observation data and microwave radiometer data were minor at the three stations, with a notable temperature inversion phenomenon observed at Sieshan Station. Regarding water vapor density, the differences between the sounding observation data and microwave radiometer data were relatively small at Sieshan Station. The relative humidity increased with height at Sieshan Station, whereas it increased and then decreased with height at SETORS and Qumalai Station. The ABLH at all sites reached its maximum value around noon, approximately 1500 m, and exhibited mostly convective boundary layer (CBL) characteristics. During the night, the ABLH mostly showed a stable boundary layer (SBL) pattern, with heights around 250 m. In summer, latent heat flux (LE) and sensible heat flux (H) in the eastern plateau were generally lower than those in the western plateau except at 20:00, where they were higher. Vertical velocity (w) in the eastern plateau was greater than in the western plateau. Among Sieshan Station and SETORS, LE, and H had the most significant impact on ABLH, while at Qumalai Station, ABLH was more influenced by surface long-wave radiation (Rlu). These four influencing factors showed a positive correlation with ABLH. The impact of different underlying surface types on ABLH primarily manifests in surface temperature variations, solar radiation intensity, vegetation cover, and terrain. Grasslands typically exhibit a larger range of ABLH variations, while the ABLH in forests and mountainous cropland areas is relatively stable. Full article

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