The 2023 MDPI Annual Report has
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15 pages, 580 KiB  
Article
eDNA Metabarcoding Reveals the Species–Area Relationship of Amphibians on the Zhoushan Archipelago
by Wenhao Li, Xianglei Hou, Yunlong Zhu, Jiacong Du, Chunxia Xu, Jingyuan Yang and Yiming Li
Animals 2024, 14(11), 1519; https://doi.org/10.3390/ani14111519 (registering DOI) - 21 May 2024
Abstract
The species–area relationship is important for understanding species diversity patterns at spatial scales, but few studies have examined the relationship using environmental DNA (eDNA) techniques. We investigated amphibian diversity on 21 islands of the Zhoushan Archipelago and nearby mainland areas in China using [...] Read more.
The species–area relationship is important for understanding species diversity patterns at spatial scales, but few studies have examined the relationship using environmental DNA (eDNA) techniques. We investigated amphibian diversity on 21 islands of the Zhoushan Archipelago and nearby mainland areas in China using the combination of eDNA metabarcoding and the traditional line transect method (TLTM) and identified the species–area relationship for amphibians on the islands. The mean detection probability of eDNA is 0.54, while the mean detection probability of TLTM is 0.24. The eDNA metabarcoding detected eight amphibian species on the islands and nine species in the mainland areas, compared with seven species on the islands and nine species in the mainland areas that were identified by TLTM. Amphibian richness on the islands increased with island area and habitat diversity. The species–area relationship for amphibians in the archipelago was formulated as the power function (S = 0.47A0.21) or exponential function (S = 2.59 + 2.41 (logA)). Our results suggested that eDNA metabarcoding is more sensitive for the detection of amphibian species. The combined use of eDNA metabarcoding and the traditional line transect method may optimize the survey results for amphibians. Full article
(This article belongs to the Special Issue Evolution, Diversity, and Conservation of Herpetofauna)
11 pages, 1161 KiB  
Article
Purpureocillium jiangxiense sp. nov.: Entomopathogenic Effects on Ostrinia furnacalis and Galleria mellonella
by Wei Chen, Yanhong Tang, Tongyi Liu, Hongwang Hu, Cuiyi Ou, Qiongbo Hu and Qunfang Weng
Microorganisms 2024, 12(6), 1041; https://doi.org/10.3390/microorganisms12061041 (registering DOI) - 21 May 2024
Abstract
The genus Purpureocillium is renowned for its role in biocontrol and biotechnological applications. The identification of new species within this genus is crucial for broadening our understanding of its ecological roles and potential utility in sustainable agriculture. This study aimed to characterize a [...] Read more.
The genus Purpureocillium is renowned for its role in biocontrol and biotechnological applications. The identification of new species within this genus is crucial for broadening our understanding of its ecological roles and potential utility in sustainable agriculture. This study aimed to characterize a new species of Purpureocillium, isolated from soil in eastern China, and to evaluate its bioactivity against Ostrinia furnacalis (corn moth) and Galleria mellonella (greater wax moth). We utilized morphological characterization; molecular phylogenetic analysis employing ITS, nrLSU, and tef1 genes; and bioactivity assays to identify and characterize the new species. The newly identified species, Purpureocillium jiangxiense sp. nov., displays unique morphological and genetic profiles compared to known species. Bioactivity tests showed that this species exhibits inhibitory effects against O. furnacalis and G. mellonella, highlighting its potential in biocontrol applications. By the ninth day at a spore concentration of 1 × 108 spores/mL, the mortality rate of the corn moth and greater wax moth reached 30% to 50% respectively. The discovery of P. jiangxiense sp. nov. adds to the genetic diversity known within this genus and offers a promising candidate for the development of natural biocontrol agents. It underscores the importance of continued biodiversity exploration and the potential for natural solutions in pest and disease management. Full article
(This article belongs to the Section Systems Microbiology)
16 pages, 4155 KiB  
Article
New 3D Vortex Microfluidic System Tested for Magnetic Core-Shell Fe3O4-SA Nanoparticle Synthesis
by Adelina-Gabriela Niculescu, Oana Maria Munteanu (Mihaiescu), Alexandra Cătălina Bîrcă, Alina Moroșan, Bogdan Purcăreanu, Bogdan Ștefan Vasile, Daniela Istrati, Dan Eduard Mihaiescu, Tony Hadibarata and Alexandru Mihai Grumezescu
Nanomaterials 2024, 14(11), 902; https://doi.org/10.3390/nano14110902 (registering DOI) - 21 May 2024
Abstract
This study’s main objective was to fabricate an innovative three-dimensional microfluidic platform suitable for well-controlled chemical syntheses required for producing fine-tuned nanostructured materials. This work proposes using vortex mixing principles confined within a 3D multilayered microreactor to synthesize magnetic core-shell nanoparticles with tailored [...] Read more.
This study’s main objective was to fabricate an innovative three-dimensional microfluidic platform suitable for well-controlled chemical syntheses required for producing fine-tuned nanostructured materials. This work proposes using vortex mixing principles confined within a 3D multilayered microreactor to synthesize magnetic core-shell nanoparticles with tailored dimensions and polydispersity. The newly designed microfluidic platform allowed the simultaneous obtainment of Fe3O4 cores and their functionalization with a salicylic acid shell in a short reaction time and under a high flow rate. Synthesis optimization was also performed, employing the variation in the reagents ratio to highlight the concentration domains in which magnetite is mainly produced, the formation of nanoparticles with different diameters and low polydispersity, and the stability of colloidal dispersions in water. The obtained materials were further characterized by X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, dynamic light scattering (DLS), and transmission electron microscopy (TEM), with the experimental results confirming the production of salicylic acid-functionalized iron oxide (Fe3O4-SA) nanoparticles adapted for different further applications. Full article
(This article belongs to the Special Issue Recent Advances in the Development of Nano-Biomaterials)
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20 pages, 4462 KiB  
Review
Synthesis of Mesoporous Silica Using the Sol–Gel Approach: Adjusting Architecture and Composition for Novel Applications
by Yandong Han, Lin Zhang and Wensheng Yang
Nanomaterials 2024, 14(11), 903; https://doi.org/10.3390/nano14110903 (registering DOI) - 21 May 2024
Abstract
The sol–gel chemistry of silica has long been used for manipulating the size, shape, and microstructure of mesoporous silica particles. This manipulation is performed in mild conditions through controlling the hydrolysis and condensation of silicon alkoxide. Compared to amorphous silica particles, the preparation [...] Read more.
The sol–gel chemistry of silica has long been used for manipulating the size, shape, and microstructure of mesoporous silica particles. This manipulation is performed in mild conditions through controlling the hydrolysis and condensation of silicon alkoxide. Compared to amorphous silica particles, the preparation of mesoporous silica, such as MCM-41, using the sol–gel approach offers several unique advantages in the fields of catalysis, medicament, and environment, due to its ordered mesoporous structure, high specific surface area, large pore volume, and easily functionalized surface. In this review, our primary focus is on the latest research related to the manipulation of mesoporous silica architectures using the sol–gel approach. We summarize various structures, including hollow, yolk-shell, multi-shelled hollow, Janus, nanotubular, and 2D membrane structures. Additionally, we survey sol–gel strategies involving the introduction of various functional elements onto the surface of mesoporous silica to enhance its performance. Furthermore, we outline the prospects and challenges associated with mesoporous silica featuring different structures and functions in promising applications, such as high-performance catalysis, biomedicine, wastewater treatment, and CO2 capture. Full article
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19 pages, 786 KiB  
Review
Role of Mesenchymal Stem/Stromal Cells (MSCs) and MSC-Derived Extracellular Vesicles (EVs) in Prevention of Telomere Length Shortening, Cellular Senescence, and Accelerated Biological Aging
by Myrna Y. Gonzalez Arellano, Matthew VanHeest, Sravya Emmadi, Amal Abdul-Hafez, Sherif Abdelfattah Ibrahim, Ranga P. Thiruvenkataramani, Rasha S. Teleb, Hady Omar, Tulasi Kesaraju, Tarek Mohamed, Burra V. Madhukar and Said A. Omar
Bioengineering 2024, 11(6), 524; https://doi.org/10.3390/bioengineering11060524 (registering DOI) - 21 May 2024
Abstract
Biological aging is defined as a progressive decline in tissue function that eventually results in cell death. Accelerated biologic aging results when the telomere length is shortened prematurely secondary to damage from biological or environmental stressors, leading to a defective reparative mechanism. Stem [...] Read more.
Biological aging is defined as a progressive decline in tissue function that eventually results in cell death. Accelerated biologic aging results when the telomere length is shortened prematurely secondary to damage from biological or environmental stressors, leading to a defective reparative mechanism. Stem cells therapy may have a potential role in influencing (counteract/ameliorate) biological aging and maintaining the function of the organism. Mesenchymal stem cells, also called mesenchymal stromal cells (MSCs) are multipotent stem cells of mesodermal origin that can differentiate into other types of cells, such as adipocytes, chondrocytes, and osteocytes. MSCs influence resident cells through the secretion of paracrine bioactive components such as cytokines and extracellular vesicles (EVs). This review examines the changes in telomere length, cellular senescence, and normal biological age, as well as the factors contributing to telomere shortening and accelerated biological aging. The role of MSCs—especially those derived from gestational tissues—in prevention of telomere shortening (TS) and accelerated biological aging is explored. In addition, the strategies to prevent MSC senescence and improve the antiaging therapeutic application of MSCs and MSC-derived EVs in influencing telomere length and cellular senescence are reviewed. Full article
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22 pages, 1037 KiB  
Article
The Nexus between Green Transformational Leadership, Employee Behavior, and Organizational Support in the Hospitality Industry
by Ibrahim A. Elshaer, Alaa M. S. Azazz, Yahdih Semlali, Mahmoud A. Mansour, Mohammed N. Elziny and Sameh Fayyad
Adm. Sci. 2024, 14(6), 107; https://doi.org/10.3390/admsci14060107 (registering DOI) - 21 May 2024
Abstract
This research paper explores the relationship between green transformational leadership (GTL), employee green behavior (EGB), and the mediating role of green organizational support (GOS) in fostering environmentally friendly practices within organizations. Drawing upon Resource-Based View Theory (RBV) and social exchange theory, the study [...] Read more.
This research paper explores the relationship between green transformational leadership (GTL), employee green behavior (EGB), and the mediating role of green organizational support (GOS) in fostering environmentally friendly practices within organizations. Drawing upon Resource-Based View Theory (RBV) and social exchange theory, the study investigates how leaders’ behaviors and organizational support influence employees’ engagement in pro-environmental initiatives. Using a quantitative research approach, data were collected from a sample of 350 hotel employees through self-administered surveys. CB-SEM was employed to analyze the hypothesized relationships among the variables. The findings indicate a significant positive relationship between GTL and EGB, suggesting that leaders who demonstrate environmentally conscious behaviors and inspire their followers to embrace sustainability principles are more likely to foster green practices among employees. Furthermore, the study reveals that GOS mediates the relationship between GTL and EGB, highlighting the importance of organizational policies, resources, and initiatives in facilitating environmentally responsible actions. These results contribute to the existing literature by shedding light on the mechanisms through which leadership and organizational support can promote sustainability initiatives in the hotel industry. Practical implications for hotels include the importance of fostering a culture of environmental consciousness, providing training and resources to support green initiatives, and empowering leaders to exemplify and promote green behaviors among employees. Full article
(This article belongs to the Special Issue Leadership and Sustainability: Building a Better Future)
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16 pages, 3791 KiB  
Article
The Intramolecular Charge Transfer Mechanism by Which Chiral Self-Assembled H8-BINOL Vesicles Enantioselectively Recognize Amino Alcohols
by Rong Wang, Kaiyue Song, Zhaoqin Wei, Yue Sun, Xiaoxia Sun and Yu Hu
Int. J. Mol. Sci. 2024, 25(11), 5606; https://doi.org/10.3390/ijms25115606 (registering DOI) - 21 May 2024
Abstract
The chiral H8-BINOL derivatives R-1 and R-2 were efficiently synthesized via a Suzuki coupling reaction, and they can be used as novel dialdehyde fluorescent probes for the enantioselective recognition of R/S-2-amino-1-phenylethanol. In addition, R-1 is much more effective than R-2. Scanning [...] Read more.
The chiral H8-BINOL derivatives R-1 and R-2 were efficiently synthesized via a Suzuki coupling reaction, and they can be used as novel dialdehyde fluorescent probes for the enantioselective recognition of R/S-2-amino-1-phenylethanol. In addition, R-1 is much more effective than R-2. Scanning electron microscope images and X-ray analyses show that R-1 can form supramolecular vesicles through the self-assembly effect of the π-π force and strong hydrogen bonding. As determined via analysis, the fluorescence of the probe was significantly enhanced by mixing a small amount of S-2-amino-1-phenylethanol into R-1, with a redshift of 38 nm, whereas no significant fluorescence response was observed in R-2-amino-1-phenylethanol. The enantioselective identification of S-2-amino-1-phenylethanol by the probe R-1 was further investigated through nuclear magnetic titration and fluorescence kinetic experiments and DFT calculations. The results showed that this mechanism was not only a simple reactive probe but also realized object recognition through an ICT mechanism. As the intramolecular hydrogen bond activated the carbonyl group on the probe R-1, the carbonyl carbon atom became positively charged. As a strong nucleophile, the amino group of S-2-amino-1-phenylethanol first transferred the amino electrons to a carbonyl carbocation, resulting in a significantly enhanced fluorescence of the probe R-1 and a 38 nm redshift. Similarly, S-2-amino-1-phenylethanol alone caused severe damage to the self-assembled vesicle structure of the probe molecule itself due to its spatial structure, which made R-1 highly enantioselective towards it. Full article
(This article belongs to the Special Issue Recent Advances in Luminescence: From Mechanisms to Applications)
20 pages, 1640 KiB  
Article
Biocompatibility and Corrosion Resistance of Si/ZrO2 Bioceramic Coating on AZ91D Using Electron Beam Physical Vapor Deposition (EB-PVD) for Advanced Biomedical Applications
by Arunkumar Thirugnanasambandam, Manoj Gupta and Rama Murugapandian
Metals 2024, 14(6), 607; https://doi.org/10.3390/met14060607 (registering DOI) - 21 May 2024
Abstract
Herein, ZrO2 and Si + ZrO2 composite coatings on AZ91D alloys are deposited at a constant voltage of 8 kV and 1 Å/s deposition rate using the electron beam physical vapor deposition (EBPVD) method. Further, the samples are examined for surface [...] Read more.
Herein, ZrO2 and Si + ZrO2 composite coatings on AZ91D alloys are deposited at a constant voltage of 8 kV and 1 Å/s deposition rate using the electron beam physical vapor deposition (EBPVD) method. Further, the samples are examined for surface morphology, phase analysis, adhesion, corrosion, and antibacterial properties, as per ASTM standards. The adhesion strength of the composite (Si + ZrO2) coating nominally dropped (9%) compared to the ZrO2 coating even when the coating thickness increased by 18%. However, the composite (Si + ZrO2) coating improved wettability because silanol promotes hydrogen bonding with water molecules, which elevates the surface energy of the silica and increases its hydrophilic nature. Further, increased wettability and surface roughness have the potential to improve cell adhesion and proliferation. The corrosion potential (Ecorr) values of the coated samples exhibited a positive shift in the potentiodynamic polarization curve, indicating a substantial increase in their corrosion resistance in the artificial blood plasma (ABP) electrolyte. Similarly, SEM images of both coated corroded samples are less affected in the ABP solution, indicating that the coating mitigated heavy cracks and micropores, protecting them from corrosion. The Si + ZrO2 coatings exhibited exceptional performance in preventing bacterial infiltration by Staphylococcus aureus, thus inhibiting the subsequent formation of biofilms. In addition, these coatings demonstrate improved vitality among fibroblast cells, enabling better cellular spreading and proliferation. Full article
16 pages, 559 KiB  
Article
Sustainable Financing for Renewable Energy: Examining the Impact of Sectoral Economy on Renewable Energy Consumption
by Edosa Getachew, Zoltan Lakner, Goshu Desalegn, Anita Tangl and Anita Boros
Economies 2024, 12(6), 127; https://doi.org/10.3390/economies12060127 (registering DOI) - 21 May 2024
Abstract
This study examines the effect of international financial flows, including investments and development assistance, on the expansion of renewable energy technologies. It also seeks to investigate the impact of the sectoral economy on the proportion of renewable energy consumption in Ethiopia. This study [...] Read more.
This study examines the effect of international financial flows, including investments and development assistance, on the expansion of renewable energy technologies. It also seeks to investigate the impact of the sectoral economy on the proportion of renewable energy consumption in Ethiopia. This study used an explanatory research design and a quantitative research approach. An autoregressive distributed lag model was applied to explore the long and short-term relationship among variables. A time series of data aggregated and disaggregated ranging from 2000 to 2022 was used. According to this study, sustainable finance programs are essential for advancing and aiding renewable energy projects in the long and short term. Ethiopia’s use of renewable energy will increase as sustainable finance rises. The main economic sectors determining Ethiopia’s consumption of renewable energy in the long and short term include the manufacturing, mining and service industries. This study’s findings imply that policies focusing on providing continuous financial support and fostering international cooperation to promote the development of the manufacturing sector are needed. This could include incentives for adopting renewable energy technologies and investing in renewable energy infrastructure. On the other hand, since the service and mining industries negatively impact renewable energy use, there is a need to diversify renewable energy sources beyond these sectors. This could involve promoting renewable energy projects in other sectors, such as manufacturing, agriculture, construction and trade. Based on the findings of this study, it is suggested that policymakers carefully consider the consequences within each economic sector when formulating decisions related to renewable energy. This study is novel in presenting empirical evidence linking renewable energy use to long- and short-term economic growth. Full article
18 pages, 7913 KiB  
Article
Utilizing a Disposable Sensor with Polyaniline-Doped Multi-Walled Carbon Nanotubes to Enable Dopamine Detection in Ex Vivo Mouse Brain Tissue Homogenates
by Thenmozhi Rajarathinam, Sivaguru Jayaraman, Jaeheon Seol, Jaewon Lee and Seung-Cheol Chang
Biosensors 2024, 14(6), 262; https://doi.org/10.3390/bios14060262 (registering DOI) - 21 May 2024
Abstract
Disposable sensors are inexpensive, user-friendly sensing tools designed for rapid single-point measurements of a target. Disposable sensors have become more and more essential as diagnostic tools due to the growing demand for quick, easy-to-access, and reliable information related to the target. Dopamine (DA), [...] Read more.
Disposable sensors are inexpensive, user-friendly sensing tools designed for rapid single-point measurements of a target. Disposable sensors have become more and more essential as diagnostic tools due to the growing demand for quick, easy-to-access, and reliable information related to the target. Dopamine (DA), a prevalent catecholamine neurotransmitter in the human brain, is associated with central nervous system activities and directly promotes neuronal communication. For the sensitive and selective estimation of DA, an enzyme-free amperometric sensor based on polyaniline-doped multi-walled carbon nanotubes (PANI-MWCNTs) drop-coated disposable screen-printed carbon electrodes (SPCEs) was fabricated. This PANI-MWCNTs-2/SPCE sensor boasts exceptional accuracy and sensitivity when working directly with ex vivo mouse brain homogenates. The sensor exhibited a detection limit of 0.05 μM (S/N = 3), and a wide linear range from 1.0 to 200 μM. The sensor’s high selectivity to DA amidst other endogenous interferents was recognized. Since the constructed sensor is enzyme-free yet biocompatible, it exhibited high stability in DA detection using ex vivo mouse brain homogenates extracted from both Parkinson’s disease and control mice models. This research thus presents new insights into understanding DA release dynamics at the tissue level in both of these models. Full article
(This article belongs to the Special Issue Biosensing Applications for Cell Monitoring)
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10 pages, 369 KiB  
Review
Biomarkers and Signaling Pathways Implicated in the Pathogenesis of Idiopathic Multicentric Castleman Disease/Thrombocytopenia, Anasarca, Fever, Reticulin Fibrosis, Renal Insufficiency, and Organomegaly (TAFRO) Syndrome
by Remi Sumiyoshi, Tomohiro Koga and Atsushi Kawakami
Biomedicines 2024, 12(6), 1141; https://doi.org/10.3390/biomedicines12061141 (registering DOI) - 21 May 2024
Abstract
Idiopathic multicentric Castleman disease (iMCD) and TAFRO syndrome present a variety of symptoms thought to be caused by excessive inflammatory cytokines and chemokines, but the underlying mechanisms are unknown. iMCD is broadly classified into two types: iMCD-NOS and iMCD-TAFRO, which have distinct laboratory [...] Read more.
Idiopathic multicentric Castleman disease (iMCD) and TAFRO syndrome present a variety of symptoms thought to be caused by excessive inflammatory cytokines and chemokines, but the underlying mechanisms are unknown. iMCD is broadly classified into two types: iMCD-NOS and iMCD-TAFRO, which have distinct laboratory findings, pathological features, and responses to treatments. It is thought that iMCD-NOS, particularly the IPL type, responds favorably to IL-6 inhibitors due to its IL-6-centric profile. iMCD-TAFRO frequently progresses acutely and seriously, similar to TAFRO syndrome. Elevated levels of cytokines, including IL-1β, TNF-α, IL-10, and IL-23, as well as chemokines like CXCL13 and CXCL-10 (especially in iMCD-TAFRO), SAA, and VEGF, have been linked to the disease’s pathology. Recent research has identified key signaling pathways including PI3K/Akt/mTOR and JAK-STAT3, as well as those regulated by type I IFN, as crucial in iMCD-TAFRO. These results suggest that dominant pathways may vary between subtypes. Further research into the peripheral blood and lymph nodes is required to determine the disease spectrum of iMCD-NOS/iMCD-TAFRO/TAFRO syndrome. Full article
(This article belongs to the Section Gene and Cell Therapy)
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10 pages, 420 KiB  
Article
The Freehand Technique: The Ability of the Human Eye to Identify Implant Sites on the Patient
by Enzo Cumbo, Giuseppe Gallina, Pietro Messina, Luigi Caradonna and Giuseppe Alessandro Scardina
Prosthesis 2024, 6(3), 551-560; https://doi.org/10.3390/prosthesis6030039 (registering DOI) - 21 May 2024
Abstract
In implantology, among the key choices, to obtain predictable results, it is essential to establish, using cone beam computed tomography (CBCT), the bone site and where to insert the implants; during the surgical phase, these sites must be identified on the oral mucosa. [...] Read more.
In implantology, among the key choices, to obtain predictable results, it is essential to establish, using cone beam computed tomography (CBCT), the bone site and where to insert the implants; during the surgical phase, these sites must be identified on the oral mucosa. Surgical templates are a valid aid, especially in complex cases which require the insertion of more than three or four implants. In cases of a single implant, on the other hand, surgical guides are rarely used, and the implant is often inserted freehand; therefore, the identification of the implant site on the oral mucosa (after choosing the location on the CBCT) is more difficult. For this reason, the clinician uses the teeth in the arch as a reference. This study evaluates the ability of the human eye to identify, on the oral mucosa, where the implant collars will be positioned, the position of which has previously been chosen on the CBCT, in cases where the hands-free surgical technique (without surgical guides) is used. The verification of this precision is carried out using particular thermo-printed templates which contain radiopaque metal spheres. The results show that, in the freehand technique, it is difficult to precisely identify the implant sites (chosen via X-ray) on the mucosa, especially when they are far from natural teeth adjacent to the edentulous area. In case of monoedentulism, the freehand implant technique seems to be applicable by expert implantologists with a reduced risk of error; in fact, clinical experience helps to find the correct correspondence between the implant site chosen on the CBCT and its identification on the mucosa. The level of experience is fundamental in the clinician’s decision about whether or not to use surgical guides; in fact, doctors with little experience should use surgical guides even in the simplest cases to reduce the risk of error. Full article
11 pages, 1229 KiB  
Systematic Review
The Risk of Aircraft-Acquired SARS-CoV-2 Transmission during Commercial Flights: A Systematic Review
by Diana Zhao, Stephanie Cheng, Fuchiang R. Tsui, Maya B. Mathur and Chih-Hung Jason Wang
Int. J. Environ. Res. Public Health 2024, 21(6), 654; https://doi.org/10.3390/ijerph21060654 (registering DOI) - 21 May 2024
Abstract
The aircraft-acquired transmission of SARS-CoV-2 poses a public health risk. Following PRISMA guidelines, we conducted a systematic review and analysis of articles, published prior to vaccines being available, from 24 January 2020 to 20 April 2021 to identify factors important for transmission. Articles [...] Read more.
The aircraft-acquired transmission of SARS-CoV-2 poses a public health risk. Following PRISMA guidelines, we conducted a systematic review and analysis of articles, published prior to vaccines being available, from 24 January 2020 to 20 April 2021 to identify factors important for transmission. Articles were included if they mentioned index cases and identifiable flight duration, and excluded if they discussed non-commercial aircraft, airflow or transmission models, cases without flight data, or that were unable to determine in-flight transmission. From the 15 articles selected for in-depth review, 50 total flights were analyzed by flight duration both as a categorical variable—short (<3 h), medium (3–6 h), or long flights (>6 h)—and as a continuous variable with case counts modeled by negative binomial regression. Compared to short flights without masking, medium and long flights without masking were associated with 4.66-fold increase (95% CI: [1.01, 21.52]; p < 0.0001) and 25.93-fold increase in incidence rates (95% CI: [4.1, 164]; p < 0.0001), respectively; long flights with enforced masking had no transmission reported. A 1 h increase in flight duration was associated with 1.53-fold (95% CI: [1.19, 1.66]; p < 0.001) increase in the incidence rate ratio (IRR) of cases. Masking should be considered for long flights. Full article
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23 pages, 3700 KiB  
Review
Exploring Port–City Relationships: A Bibliometric and Content Analysis
by Melika Zarei, Mojtaba Arasteh and Sina Shahab
Sustainability 2024, 16(11), 4341; https://doi.org/10.3390/su16114341 (registering DOI) - 21 May 2024
Abstract
Planning for port development presents a complex challenge. Along with fostering cooperation and economic synergies, port development also necessitates careful balancing with existing city functions. Previous research on port–city relationships (PCRs) has yielded valuable insights, but a comprehensive systematic and bibliometric review to [...] Read more.
Planning for port development presents a complex challenge. Along with fostering cooperation and economic synergies, port development also necessitates careful balancing with existing city functions. Previous research on port–city relationships (PCRs) has yielded valuable insights, but a comprehensive systematic and bibliometric review to identify future research directions is lacking. Moreover, existing research in this field has not addressed the need for a comprehensive classification of content, methods, and driving forces. This study addresses these gaps by analyzing 113 academic articles published on PCRs between 2000 and 2023. Using a bibliometric approach, this study leverages the JavaScript programming language, VOSviewer 1.6.20 software, and the Scopus database. This paper aims to identify key research areas and influential studies within the field of PCRs. This analysis reveals emerging research topics and themes within PCRs. Additionally, it depicts which research countries, journals, and keywords are the most prominent with regard to this field, outlining the relationships between them. This study’s findings provide valuable insights into the current state of PCR research, including geographical distribution, publication trends, methodological approaches, and key research variables. Full article
(This article belongs to the Section Sustainable Urban and Rural Development)
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15 pages, 3721 KiB  
Article
Prediction Distribution Model of Moisture Content in Laminated Wood Components
by Panpan Tian, Jianhong Han, Shangjie Guo, Jun Di and Xia Han
Polymers 2024, 16(11), 1453; https://doi.org/10.3390/polym16111453 (registering DOI) - 21 May 2024
Abstract
Shrinkage cracks are some of the most common defects in timber structures obtained from woods with an uneven distribution of moisture content and are subject to external dynamic environmental changes. To accurately predict the changes in the moisture content of wood components at [...] Read more.
Shrinkage cracks are some of the most common defects in timber structures obtained from woods with an uneven distribution of moisture content and are subject to external dynamic environmental changes. To accurately predict the changes in the moisture content of wood components at any time and position, this study first applied the principles of food drying and established a moisture field model for laminated wood based on the analogy between heat and humidity transfer. A model for predicting the moisture content of wood that considers time and spatial distribution was then proposed. Second, by collecting relevant experimental data and establishing a finite element analysis model, three moisture absorption conditions (0–9.95%, 0–13.65%, and 0–17.91%) and four desorption conditions (34–5.5%, 28–8.3%, 31–11.8%, and 25.5–15.9%) were analyzed. In the moisture absorption comparison, the time needed to reach 95% equilibrium moisture content was 2.43 days, 4.07 days, and 6.32 days. The rate at which the internal components reached equilibrium moisture content exceeded 10 days. The temporal and spatial distribution of wood moisture content revealed the correctness of the proposed wood moisture field model. Finally, the moisture content prediction model was applied in the order of characteristic equation solutions, moisture content gradient difference, and laminated wood size. The results revealed that the established humidity field model can predict the wood moisture content and how it changes over time and in space. Notably, 1–2 orders for the solution of the characteristic equation are recommended when applying the prediction model. The greater the difference in moisture content, the faster the equilibrium moisture content is reached. The moisture content varies greatly based on the component size and position. Notably, the influence of moisture gradient and wood size on the average wood moisture content cannot be ignored. Full article
(This article belongs to the Section Polymer Physics and Theory)
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17 pages, 817 KiB  
Article
Biomolecule-Producing Probiotic Bacterium Lactococcus lactis in Free or Nanoencapsulated Form for Endometritis Treatment and Fertility Improvement in Buffaloes
by Nesrein M. Hashem, Walaa M. Essawi, Azza S. El-Demerdash and Ali Ali El-Raghi
J. Funct. Biomater. 2024, 15(6), 138; https://doi.org/10.3390/jfb15060138 (registering DOI) - 21 May 2024
Abstract
A Lactococcus (L.) lactis strain producing antimicrobial and anti-inflammatory biomolecules (mainly 1,4-Diaza-2,5-dioxobicyclo[4.3.0]nonanes and pyrazine-derivatives) was tested for its capacity to cure clinical endometritis in buffaloes compared to conventional antibiotic-based treatment. Clinical endometritis-diagnosed buffaloes (n = 16/group) were infused intrauterine with four [...] Read more.
A Lactococcus (L.) lactis strain producing antimicrobial and anti-inflammatory biomolecules (mainly 1,4-Diaza-2,5-dioxobicyclo[4.3.0]nonanes and pyrazine-derivatives) was tested for its capacity to cure clinical endometritis in buffaloes compared to conventional antibiotic-based treatment. Clinical endometritis-diagnosed buffaloes (n = 16/group) were infused intrauterine with four doses of 109 CFU-free (FLC group) or nanoencapsulated L. lactis (NLC group) and compared to those that received three doses of saline + a single dose of 500 mg cephapirin benzathin (AB group) or four doses of saline (control, C group) every other day. Endometrium samples were analyzed for cytological (polymorphonuclear cells, PMN), bacteriological, and proinflammatory mRNA expression. Uterine wash and blood samples were collected to determine proinflammatory cytokine concentrations and metabolites in the blood samples. The reproductive performance of buffaloes was assessed. Compared to the C group, the AB and NLC groups had the lowest percentage of PMN, followed by those in the FLC group (p < 0.05). All treated buffaloes had significantly lower numbers of pathogens than the control buffaloes. Compared to control, all treatments significantly down-regulated endometrial proinflammatory encoding mRNA expression. The concentrations of IL1B, TNFAIP7, and leukocyte esterase activity in the uterine washings were significantly decreased in the AB and NLC groups compared to the C and FLC groups. All treatments significantly decreased concentrations of serum proinflammatory cytokines compared to control. Both the AB and NLC groups had significantly lower concentrations of serum NEFA than the C and FLC groups. The percentage of control buffaloes having an echogenic uterus and PVD score > 2 was significantly higher than those in the treated buffaloes with higher numbers of corpora lutea, higher conception rates, and shorter days open than control buffaloes (p < 0.05). In conclusion, L. lactis-producing antimicrobial and anti-inflammatory metabolites reduce uterine inflammatory responses and improve fertility in buffaloes. Full article
21 pages, 28792 KiB  
Article
Imaging and Interferometric Mapping Exploration for PIESAT-01: The World’s First Four-Satellite “Cartwheel” Formation Constellation
by Tian Zhang, Yonggang Qian, Chengming Li, Jufeng Lu, Jiao Fu, Qinghua Guo, Shibo Guo and Yuxiang Wang
Atmosphere 2024, 15(6), 621; https://doi.org/10.3390/atmos15060621 (registering DOI) - 21 May 2024
Abstract
The PIESAT-01 constellation is the world’s first multi-baseline distributed synthetic aperture radar (SAR) constellation with a “Cartwheel” formation. The “Cartwheel” formation is a unique formation in which four satellites fly in companion orbits, ensuring that at any given moment, the main satellite remains [...] Read more.
The PIESAT-01 constellation is the world’s first multi-baseline distributed synthetic aperture radar (SAR) constellation with a “Cartwheel” formation. The “Cartwheel” formation is a unique formation in which four satellites fly in companion orbits, ensuring that at any given moment, the main satellite remains at the center, with three auxiliary satellites orbiting around it. Due to this unique configuration of the PIESAT-01 constellation, four images of the same region and six pairs of baselines can be obtained with each shot. So far, there has been no imaging and interference research based on four-satellite constellation measured data, and there is an urgent need to explore algorithms for the “Cartwheel” configuration imaging and digital surface model (DSM) production. This paper introduces an improved bistatic SAR imaging algorithm under the four-satellites interferometric mode, which solves the problem of multi-orbit nonparallelism in imaging while ensuring imaging coherence and focusing ability. Subsequently, it presents an interferometric processing method for the six pairs of baselines, weighted fusion based on elevation ambiguity from different baselines, to obtain a high-precision DSM. Finally, this paper selects the Dingxi region of China and other regions with diverse terrains for imaging and DSM production and compares the DSM results with ICESat-2 global geolocated photon data and TanDEM DSM data. The results indicate that the accuracy of PIESAT-01 DSM meets the standards of China’s 1:50,000 scale and HRTI-3, demonstrating a high level of precision. Moreover, PIESAT-01 data alleviate the reliance on simulated data for research on multi-baseline imaging and multi-baseline phase unwrapping algorithms and can provide more effective and realistic measured data. Full article
(This article belongs to the Special Issue Land Surface Processes: Modeling and Observation)
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18 pages, 1077 KiB  
Article
Determinants of Butterfly Community Structure and Composition at the Local Habitat Level: Importance of Neighboring Vegetation and Management Status: A Case Study
by Masahiko Kitahara and Taisuke Yasuda
Diversity 2024, 16(6), 310; https://doi.org/10.3390/d16060310 (registering DOI) - 21 May 2024
Abstract
Exploring the determinants of biological community structure is important not only for understanding the formation of the community, but also for promoting its biodiversity conservation. We monitored butterfly communities in a grassland and woodland area of Mount Fuji, Japan, and explored the environmental [...] Read more.
Exploring the determinants of biological community structure is important not only for understanding the formation of the community, but also for promoting its biodiversity conservation. We monitored butterfly communities in a grassland and woodland area of Mount Fuji, Japan, and explored the environmental factors that influenced them. Multivariate regression tree (MRT) analysis generated a 3-leaved MRT (G1, G2, and G3) and showed the total explained variation of 64.4% in the species composition. The explanatory variables discriminating between the two branches in the first node and in the second node of the MRT were neighboring plant community and management status, respectively. The neighboring plant community was related to the distribution of butterfly dietary resources and contributed a lot in determining the species composition of the community while the management status was largely related to the amount of their dietary resources, affecting the species richness and diversity. The PCA results revealed that the three key groups (G1, G2, and G3) were formed along the gradients of these two factors. Consequently, maintaining mowing management and the diversity of neighboring vegetation is very important for the continuance of butterfly community diversity and composition and the conservation of Red Listed species in the area. Full article
(This article belongs to the Special Issue Biodiversity, Ecology and Conservation of Lepidoptera)
14 pages, 925 KiB  
Article
Forecasting and Anomaly Detection in BEWS: Comparative Study of Theta, Croston, and Prophet Algorithms
by Aleksandr N. Grekov, Elena V. Vyshkvarkova and Aleksandr S. Mavrin
Forecasting 2024, 6(2), 343-356; https://doi.org/10.3390/forecast6020019 (registering DOI) - 21 May 2024
Abstract
Evaluation of water quality and accurate prediction of water pollution indicators are key components in water resource management and water pollution control. The use of biological early warning systems (BEWS), in which living organisms are used as biosensors, allows for a comprehensive assessment [...] Read more.
Evaluation of water quality and accurate prediction of water pollution indicators are key components in water resource management and water pollution control. The use of biological early warning systems (BEWS), in which living organisms are used as biosensors, allows for a comprehensive assessment of the aquatic environment state and a timely response in the event of an emergency. In this paper, we examine three machine learning algorithms (Theta, Croston and Prophet) to forecast bivalves’ activity data obtained from the BEWS developed by the authors. An algorithm for anomalies detection in bivalves’ activity data was developed. Our results showed that for one of the anomalies, Prophet was the best method, and for the other two, the anomaly detection time did not differ between the methods. A comparison of methods in terms of computational speed showed the advantage of the Croston method. This anomaly detection algorithm can be effectively incorporated into the software of biological early warning systems, facilitating rapid responses to changes in the aquatic environment. Full article
37 pages, 8813 KiB  
Article
Experimental and Numerical Study of Suspended Inter-Array Cable Configurations for Floating Offshore Wind Farm
by Di-Rong Li, Yu-Shiou Su and Ray-Yeng Yang
J. Mar. Sci. Eng. 2024, 12(6), 853; https://doi.org/10.3390/jmse12060853 (registering DOI) - 21 May 2024
Abstract
The present study evaluates the feasibility of using a fully suspended inter-array cable system for an offshore wind farm. It includes both numerical simulations and a scaled-down experiment, conducted at a 1:49 scale, to validate the numerical results. To achieve the goal, a [...] Read more.
The present study evaluates the feasibility of using a fully suspended inter-array cable system for an offshore wind farm. It includes both numerical simulations and a scaled-down experiment, conducted at a 1:49 scale, to validate the numerical results. To achieve the goal, a 15 MW floating offshore wind turbine (FOWT) and a floating offshore substation (FOSS) are involved to simulate the wind farm array. This study incorporates the 50-year return period conditions of the Taiwan Hsinchu offshore area, which has a water depth of about 100 m, to validate the specifications related to the platform motion and mooring line tension. Additionally, an analysis of the tension, curvature, and fatigue damage of the dynamic cable system is discussed in this research. Because a fully suspended cable is a relatively new concept and may be more frequently considered in a deeper water depth area, numerical simulation software Orcina Orcaflex 11.4 has been chosen to conduct the fully coupled simulation, determining whether the fully suspended cable system could effectively withstand the challenges posed by extreme sea conditions. This is due to the reason that a fully suspended cable would occupy a larger space in the ocean, which may pose a risk by influencing the navigation of the vessels. Therefore, the cable laying depth under normal sea states is also discussed to evaluate the influence over vessel navigation. This study also collects the long-term environmental data from the Central Weather Bureau, Taiwan, to calculate the accumulative cable fatigue damage under different sea states. To integrate the results, this research applies fitness parameters to evaluate the feasibility of each cable configuration. Covering the cable performance under extreme sea states and regular operating sea states offers valuable insights for applications in ocean engineering. Full article
(This article belongs to the Special Issue New Era in Offshore Wind Energy)
14 pages, 1302 KiB  
Article
Analysis of the Driving Range Evaluation Method for Fuel-Cell Electric Vehicles
by Ting Guo, Letian Sun, Guozhuo Wang and Shiyu Wu
World Electr. Veh. J. 2024, 15(6), 223; https://doi.org/10.3390/wevj15060223 (registering DOI) - 21 May 2024
Abstract
The range is one of the most important performance indicators for fuel-cell electric vehicles. This article focuses on the analysis of GB/T 43252-2023 “Energy Consumption and Range Test Methods for Fuel-Cell Electric Vehicles” from the perspective of a standard analysis, and conducts actual [...] Read more.
The range is one of the most important performance indicators for fuel-cell electric vehicles. This article focuses on the analysis of GB/T 43252-2023 “Energy Consumption and Range Test Methods for Fuel-Cell Electric Vehicles” from the perspective of a standard analysis, and conducts actual vehicle tests on the range test method and process. It introduces the measurement method of hydrogen gas filling for test vehicles, and explains the main content of the new standard revision and the main differences between the new and old standards. This article takes the fuel-cell dump truck as an example, and analyzed the relationship between the output power of fuel-cell stacks and power batteries during vehicle operation and driving conditions, as well as the proportion of fuel cell output power. The results show that the optimal output power range of fuel cells is 20–40 kW, accounting for 45.2% of the total operating time. When driving at high speeds, the output power of fuel cells is greater than that of power batteries. Full article
23 pages, 1830 KiB  
Article
Discovery of Natural Compound-Based Lead Molecule against Acetyltransferase Type 1 Bacterial Enzyme from Morganella morgani Using Machine Learning-Enabled Molecular Dynamics Simulation
by Meshari Alazmi and Olaa Motwalli
Processes 2024, 12(6), 1047; https://doi.org/10.3390/pr12061047 (registering DOI) - 21 May 2024
Abstract
Drug-resistant Morganella morganii, a rod-shaped, Gram-negative, facultatively anaerobic bacillus belonging to the Enterobacteriaceae family, is a growing worldwide health concern due to its association with high morbidity and mortality rates. Recent advancements in machine learning, particularly Alphafold 2′s protein structure prediction using [...] Read more.
Drug-resistant Morganella morganii, a rod-shaped, Gram-negative, facultatively anaerobic bacillus belonging to the Enterobacteriaceae family, is a growing worldwide health concern due to its association with high morbidity and mortality rates. Recent advancements in machine learning, particularly Alphafold 2′s protein structure prediction using local physics and pattern recognition, have aided research efforts. This study focuses on the enzymatic activity of aminoglycoside N6′-acetyltransferase (aacA7), a critical transferase enzyme in bacteria that confers resistance to aminoglycosides. AacA7 modifies aminoglycoside molecules by catalyzing the acetylation of their 6′-amino group using acetyl-CoA, rendering antibiotics like kanamycin, neomycin, tobramycin, and amikacin inactive. We propose that Doripenem and OncoglabrinolC can interact with aacA7, potentially modifying its enzymatic activity. Molecular docking analysis of aacA7 with 22 drug targets revealed OncoglabrinolC as the most promising candidate, exhibiting a binding energy of −12.82 kcal/mol. These two top candidates, OncoglabrinolC and Doripenem, were then subjected to 100 ns of molecular dynamic simulations to assess their dynamic conformational features. Furthermore, the PredictSNP consensus classifier was used to predict the impact of mutations on aacA7 protein functionality. The study also investigated the interaction of wild-type and mutant aacA7 proteins with both Doripenem and OncoglabrinolC. These findings provide valuable insights into the binding behavior of OncoglabrinolC and Doripenem as potential lead molecules for repurposing against aacA7, potentially reducing the pathogenicity of Morganella morganii. Full article
17 pages, 735 KiB  
Article
Quality and Establishment of Some Water-Conserving Turfgrass Species for Sustainable Development and Some Ecosystem Services in Arid Urban Environments
by Fatemeh Kazemi, Mahmood Reza Golzarian and Seyedeh Maliheh Rabbani Kheir Khah
Land 2024, 13(6), 721; https://doi.org/10.3390/land13060721 (registering DOI) - 21 May 2024
Abstract
Turfgrasses are essential landscape plants with social, environmental, and aesthetic services for urban ecosystems. However, more is needed to know how to establish them so that they can benefit from their ecosystem services in urban environments. This research examined some quality and morphological [...] Read more.
Turfgrasses are essential landscape plants with social, environmental, and aesthetic services for urban ecosystems. However, more is needed to know how to establish them so that they can benefit from their ecosystem services in urban environments. This research examined some quality and morphological and physiological factors for the establishment and social and environmental service assessment of three warm-season turfgrasses, including Kikuyu grass (Pennisetum clandestinum), bermuda grass (Cynodon dactylon), and buffalo grass (Buchloe dactyloides), compared to the cool-season grass of tall fescue (Festuca arundinacea Schreb.). The experiment was split-plot in time, based on a randomized complete block design with eight replications. The main plot was the season with four levels, and the subplot was the four turfgrass species types. The results indicated that seasons and turfgrass types and their interaction significantly impacted most measured variables (p ≤ 0.01). Some quality measurements like turf density, color, texture, coverage, and quality after clipping and establishment confirmed the superiority of Buchloe dactyloides over the other species. Also, kikuyu grass showed higher turfgrass density, more potential for weed control, and higher coverage and growth rate but also showed invasiveness features. Tall fescue had the lowest visual aesthetic compared with the other turfgrass species. Warm-season turfgrasses adaptable to the ecology of the region should be used compared to tall fescue to achieve better turfgrass quality and social and ecosystem services for the sustainable development of arid urban environments. Full article

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