The 2023 MDPI Annual Report has
been released!
 
18 pages, 4434 KiB  
Article
Impact of the Transboundary Interference Inhibitor on RNAi and the Baculovirus Expression System in Insect Cells
by Hao Zheng, Hengfeng Zhao, Haifan Xiong, Mian Muhammad Awais, Songrong Zeng and Jingchen Sun
Insects 2024, 15(6), 375; https://doi.org/10.3390/insects15060375 (registering DOI) - 21 May 2024
Abstract
RNA interference inhibitors were initially discovered in plant viruses, representing a unique mechanism employed by these viruses to counteract host RNA interference. This mechanism has found extensive applications in plant disease resistance breeding and other fields; however, the impact of such interference inhibitors [...] Read more.
RNA interference inhibitors were initially discovered in plant viruses, representing a unique mechanism employed by these viruses to counteract host RNA interference. This mechanism has found extensive applications in plant disease resistance breeding and other fields; however, the impact of such interference inhibitors on insect cell RNA interference remains largely unknown. In this study, we screened three distinct interference inhibitors from plant and mammal viruses that act through different mechanisms and systematically investigated their effects on the insect cell cycle and baculovirus infection period at various time intervals. Our findings demonstrated that the viral suppressors of RNA silencing (VSRs) derived from plant and mammal viruses significantly attenuated the RNA interference effect in insect cells, as evidenced by reduced apoptosis rates, altered gene regulation patterns in cells, enhanced expression of exogenous proteins, and improved production efficiency of recombinant virus progeny. Further investigations revealed that the early expression of VSRs yielded superior results compared with late expression during RNA interference processes. Additionally, our results indicated that dsRNA-binding inhibition exhibited more pronounced effects than other modes of action employed by these interference inhibitors. The outcomes presented herein provide novel insights into enhancing defense mechanisms within insect cells using plant and mammal single-stranded RNA virus-derived interference inhibitors and have potential implications for expanding the scope of transformation within insect cell expression systems. Full article
(This article belongs to the Section Insect Molecular Biology and Genomics)
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18 pages, 1363 KiB  
Article
Unveiling the Nature and Strength of Selenium-Centered Chalcogen Bonds in Binary Complexes of SeO2 with Oxygen-/Sulfur-Containing Lewis Bases: Insights from Theoretical Calculations
by Tao Lu, Renhua Chen, Qingyu Liu, Yeshuang Zhong, Fengying Lei and Zhu Zeng
Int. J. Mol. Sci. 2024, 25(11), 5609; https://doi.org/10.3390/ijms25115609 (registering DOI) - 21 May 2024
Abstract
Among various non-covalent interactions, selenium-centered chalcogen bonds (SeChBs) have garnered considerable attention in recent years as a result of their important contributions to crystal engineering, organocatalysis, molecular recognition, materials science, and biological systems. Herein, we systematically investigated π–hole-type Se∙∙∙O/S ChBs in [...] Read more.
Among various non-covalent interactions, selenium-centered chalcogen bonds (SeChBs) have garnered considerable attention in recent years as a result of their important contributions to crystal engineering, organocatalysis, molecular recognition, materials science, and biological systems. Herein, we systematically investigated π–hole-type Se∙∙∙O/S ChBs in the binary complexes of SeO2 with a series of O-/S-containing Lewis bases by means of high-level ab initio computations. The results demonstrate that there exists an attractive interaction between the Se atom of SeO2 and the O/S atom of Lewis bases. The interaction energies computed at the MP2/aug-cc-pVTZ level range from −4.68 kcal/mol to −10.83 kcal/mol for the Se∙∙∙O chalcogen-bonded complexes and vary between −3.53 kcal/mol and −13.77 kcal/mol for the Se∙∙∙S chalcogen-bonded complexes. The Se∙∙∙O/S ChBs exhibit a relatively short binding distance in comparison to the sum of the van der Waals radii of two chalcogen atoms. The Se∙∙∙O/S ChBs in all of the studied complexes show significant strength and a closed-shell nature, with a partially covalent character in most cases. Furthermore, the strength of these Se∙∙∙O/S ChBs generally surpasses that of the C/O–H∙∙∙O hydrogen bonds within the same complex. It should be noted that additional C/O–H∙∙∙O interactions have a large effect on the geometric structures and strength of Se∙∙∙O/S ChBs. Two subunits are connected together mainly via the orbital interaction between the lone pair of O/S atoms in the Lewis bases and the BD*(OSe) anti-bonding orbital of SeO2, except for the SeO2∙∙∙HCSOH complex. The electrostatic component emerges as the largest attractive contributor for stabilizing the examined complexes, with significant contributions from induction and dispersion components as well. Full article
14 pages, 1898 KiB  
Article
Use of Continuous Stirred Tank Reactors for Anaerobic Co-Digestion of Dairy and Meat Industry By-Products for Biogas Production
by Alessandro Neri, Ferdinand Hummel, Souraya Benalia, Giuseppe Zimbalatti, Wolfgang Gabauer, Ivana Mihajlovic and Bruno Bernardi
Sustainability 2024, 16(11), 4346; https://doi.org/10.3390/su16114346 (registering DOI) - 21 May 2024
Abstract
The dairy and meat industries generate thousands of tons of organic waste and by-products each year, making them two of the least environmentally sustainable sectors. Typical waste includes not only processing by-products such as curds but also commercial products that are defective or [...] Read more.
The dairy and meat industries generate thousands of tons of organic waste and by-products each year, making them two of the least environmentally sustainable sectors. Typical waste includes not only processing by-products such as curds but also commercial products that are defective or unsaleable due to expiration or damaged packaging. This study aimed to evaluate the methanogenic potential of a mixture of 80% inedible curds and 20% expired sausages, as a substrate, using two continuously stirred tank reactors (CSTR). The reactors were fed daily with increasing doses of the 80–20% mixture and an organic loading rate ranging from 0.31 gVS/litre/day at the beginning of the trials to 7.20 gVS/litre/day toward the end. The produced biogas was continuously analysed from both quantitative and qualitative point of view. Also, the process was continuously monitored by withdrawing samples from each reactor during the whole process, to analyse their physical–chemical parameters, including pH, total solids (TS), total volatile solids (TVS), chemical oxygen demand (COD), ammonium nitrogen (NH4⁺-N), total Kjeldahl nitrogen (TKN) and total volatile fatty acids (VFA). The results of this study show a promising increase in biogas production with the increase in feed. In terms of biogas production, organic waste from the dairy and meat industry shows the potential to be exploited as a substrate to produce biomethane. Indeed, in this study, biomethane cumulative production reached 410.86 NLCH4∙gTVS−1 using an 8 L capacity reactor filled up to 6 L. This makes the tested by-products usable as a renewable energy source in the future, particularly within a circular economy approach, helping to mitigate the effects of global warming and addressing sustainable development goals. Full article
19 pages, 2606 KiB  
Article
In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product
by Riviello-Flores María de la Luz, Castillo-Martínez Carlos Román, Cadena-Iñiguez Jorge, Ruiz-Posadas Lucero del Mar, Soto-Hernández Ramón Marcos, Arévalo-Galarza Ma. de Lourdes and Castillo-Juárez Israel
Horticulturae 2024, 10(6), 537; https://doi.org/10.3390/horticulturae10060537 (registering DOI) - 21 May 2024
Abstract
Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the [...] Read more.
Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the shoot propagation, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL−1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds and shoots and the shoot height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L−1 of BA and 0.1 mg L−1 of TDZ, as well as the MS base culture medium. The validation of the results of the first phase (0.1 mg L−1 of BA) was compared with the MS in an independent experiment against the control (n = 50 repetitions), obtaining a height of 52 mm, 1.36 shoots, and 9.22 buds, suggesting that this concentration is adequate for the purpose, surpassing the MS control (MS culture medium alone). Of the total volume of roots obtained with packed bud structure in the previous experimental sample, it was reduced to 14% (n = 50). The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L−1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. Callus induction in S. compositum was better when using the stem in a medium with 2.0 mg L−1 of 2,4-D with a value of 97.8% around the explant. The addition of 500 mg L−1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callus formation of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction. Full article
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12 pages, 736 KiB  
Technical Note
Estimating Effective Light Exposure by Property-Tracking Tracers
by Edward Gross, Rusty Holleman, Eric Deleersnijder and Eric J. M. Delhez
Water 2024, 16(11), 1469; https://doi.org/10.3390/w16111469 (registering DOI) - 21 May 2024
Abstract
A numerical approach to estimating the mean exposure of a tracer to a scalar property is applied to predict the light exposure of sinking phytoplankton. In our application, effective light exposure is defined as the time integral of a light limitation factor commonly [...] Read more.
A numerical approach to estimating the mean exposure of a tracer to a scalar property is applied to predict the light exposure of sinking phytoplankton. In our application, effective light exposure is defined as the time integral of a light limitation factor commonly used in phytoplankton models. Solutions from an adjoint approach have previously been published for a set of one-dimensional sinking phytoplankton scenarios. We illustrate that a simple extension to a standard advection–diffusion model produces similar results. Specifically, we present numerical solutions using property tracking in a one-dimensional advection–diffusion model for multiple tracers. Solutions are calculated for a range of eddy diffusivity distributions and compared with the published solutions. The consistency of the numerical solutions with the published solutions provides validation of the property-tracking approach. While the adjoint method solution is much less computationally intensive for the test cases, the property-tracking approach can be applied in multidimensional time-varying applications with an arbitrary distribution of sinking speed, diffusivity, and turbidity for which an adjoint solution has not been developed. Our intention is for this example application and corroboration of the “property-tracking” approach to inspire readers to envision additional applications for this approach. Full article
(This article belongs to the Section Hydraulics and Hydrodynamics)
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12 pages, 760 KiB  
Article
Processability and Separability of Commercial Anti-Corrosion Coatings Produced by In Situ Hydrogen-Processing of Magnetic Scrap (HPMS) Recycling of NdFeB
by Laura Grau, Peter Fleissner, Spomenka Kobe and Carlo Burkhardt
Materials 2024, 17(11), 2487; https://doi.org/10.3390/ma17112487 (registering DOI) - 21 May 2024
Abstract
The recycling of NdFeB magnets is necessary to ensure a reliable and ethical supply of rare earth elements as critical raw materials. This has been recognized internationally, prompting the implementation of large-scale legislative measured aimed at its resolution; for example, an ambitious recycling [...] Read more.
The recycling of NdFeB magnets is necessary to ensure a reliable and ethical supply of rare earth elements as critical raw materials. This has been recognized internationally, prompting the implementation of large-scale legislative measured aimed at its resolution; for example, an ambitious recycling quote has been established in the Critical Raw Materials Act Successful recycling in sufficient quantities is challenged by product designs that do not allow the extraction and recycling of these high-performance permanent magnets without excessive effort and cost. This is particularly true for smaller motors using NdFeB magnets. Therefore, methods of recycling such arrangements with little or no dismantling are being researched. They are tested for the hydrogen-processing of magnetic scrap (HPMS) method, a short-loop mechanical recycling process. As contamination of the recycled material with residues of anti-corrosion coatings, adhesives, etc., may lead to downcycling, the separability of such residues from bulk magnets and magnet powder is explored. It is found that the hydrogen permeability, expansion volume, and the chosen coating affect the viable preparation and separation methods as recyclability-relevant design features. Full article
24 pages, 780 KiB  
Review
Examining Energy Efficiency Practices in Office Buildings through the Lens of LEED, BREEAM, and DGNB Certifications
by Bianca Gasparetto Rebelatto, Amanda Lange Salvia, Luciana Londero Brandli and Walter Leal Filho
Sustainability 2024, 16(11), 4345; https://doi.org/10.3390/su16114345 (registering DOI) - 21 May 2024
Abstract
Energy accounts for a significant share of carbon emissions, and buildings play a substantial role in this by contributing to both direct and indirect emissions throughout their lifecycle. Enhancing energy efficiency in buildings is a strategy to mitigate these impacts. The main goal [...] Read more.
Energy accounts for a significant share of carbon emissions, and buildings play a substantial role in this by contributing to both direct and indirect emissions throughout their lifecycle. Enhancing energy efficiency in buildings is a strategy to mitigate these impacts. The main goal of this review is to uncover solutions, trends, and examples of good practices in the field of office buildings. It presents effective cases and a SWOT analysis of LEED, BREEAM, and DGNB certifications, highlighting their contributions to energy efficiency in buildings on an international scale. The paper identifies and outlines similarities and differences between each methodology used to achieve energy efficiency in different buildings and contexts. The findings may allow new ways to improve access and obtain results regarding energy efficiency, thereby supporting building owners and companies in finding more effective solutions. The research highlights the necessity for continual enhancements in these systems, which should involve addressing economic factors, conducting post-occupancy evaluations, and considering lifecycle perspectives. The recommendations encompass standardizing practices, considering costs, conducting regular revisions, managing materials and resources, and incorporating occupancy measures. Full article
51 pages, 1461 KiB  
Review
Survey of Advanced Nonlinear Control Strategies for UAVs: Integration of Sensors and Hybrid Techniques
by Nadir Abbas, Zeshan Abbas, Samra Zafar, Naseem Ahmad, Xiaodong Liu, Saad Saleem Khan, Eric Deale Foster and Stephen Larkin
Sensors 2024, 24(11), 3286; https://doi.org/10.3390/s24113286 (registering DOI) - 21 May 2024
Abstract
This survey paper explores advanced nonlinear control strategies for Unmanned Aerial Vehicles (UAVs), including systems such as the Twin Rotor MIMO system (TRMS) and quadrotors. UAVs, with their high nonlinearity and significant coupling effects, serve as crucial benchmarks for testing control algorithms. Integration [...] Read more.
This survey paper explores advanced nonlinear control strategies for Unmanned Aerial Vehicles (UAVs), including systems such as the Twin Rotor MIMO system (TRMS) and quadrotors. UAVs, with their high nonlinearity and significant coupling effects, serve as crucial benchmarks for testing control algorithms. Integration of sophisticated sensors enhances UAV versatility, making traditional linear control techniques less effective. Advanced nonlinear strategies, including sensor-based adaptive controls and AI, are increasingly essential. Recent years have seen the development of diverse sliding surface-based, sensor-driven, and hybrid control strategies for UAVs, offering superior performance over linear methods. This paper reviews the significance of these strategies, emphasizing their role in addressing UAV complexities and outlining future research directions. Full article
(This article belongs to the Special Issue Dalian University of Technology Celebrating 75th Anniversary)
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16 pages, 609 KiB  
Article
Phytochemistry and Allelopathic Effects of Tanacetum vulgare L. (Tansy) Extracts on Lepidium sativum L. (Garden Pepper Cress) and Lactuca sativa L. (Lettuce)
by Asta Judžentienė, Jurga Būdienė, Donata Stancelytė and Irena Nedveckytė
Horticulturae 2024, 10(6), 538; https://doi.org/10.3390/horticulturae10060538 (registering DOI) - 21 May 2024
Abstract
Tanacetum vulgare is a perennial plant growing wild along roadsides, pastures, and agricultural fields. Its prevalence is due to several factors: good climatic adaptability, high self-seeding potential, phenotypic plasticity, multiplying via underground rhizomes and its allelochemicals, which influence the seed germination, root development [...] Read more.
Tanacetum vulgare is a perennial plant growing wild along roadsides, pastures, and agricultural fields. Its prevalence is due to several factors: good climatic adaptability, high self-seeding potential, phenotypic plasticity, multiplying via underground rhizomes and its allelochemicals, which influence the seed germination, root development and the overall vegetation of the surrounding plants. The phytochemistry of tansy extracts and their allelopathic activity on the seed germination and growth of garden pepper cress (Lepidium sativum L.) and lettuce (Lactuca sativa L.) were investigated. The major volatile compounds, 1,8-cineole, camphor and borneol were determined in tansy flower extracts. The leaf extracts contained appreciable amounts of 1,8-cineole and borneol. Feruloylquinic, (di)ferulic and dehydrocaffeoyl-5-caffeoylquinic acids, acacetin, ludovicin С and tanacetin were determined both in leaf and inflorescence extracts. Root extracts contained minor quantities of some terpenoids and polyphenols. Extracts of T. vulgare’s aerial parts showed strong allelopathic effects on model plants. The flower and leaf water extracts inhibited lettuce and pepper cress seed germination and growth the most. According to the fractions, the acidic solution had the strongest effect, followed by neutral and alkaline solutions. At the highest relative concentrations of 0.5 and 1.0 tansy leaf acidic fraction, lettuce seed germination and growth decreased by 89.93% (from 35.07 ± 4.79 to 3.53 ± 2.10 mm) and by 98.46% (from 35.07 ± 4.79 to 0.57 ± 0.98 mm) compared to the control, respectively. Tansy root extracts showed weak effects. Our results demonstrated that the allelopathic inhibitory potential of tansy extracts was higher on garden pepper cress than on lettuce. The presence of allelochemicals in T. vulgare may have a significant impact on plant communities and ecosystems. Full article
29 pages, 5724 KiB  
Review
Smartphone Prospects in Bridge Structural Health Monitoring, A Literature Review
by Ekin Ozer and Rolands Kromanis
Sensors 2024, 24(11), 3287; https://doi.org/10.3390/s24113287 (registering DOI) - 21 May 2024
Abstract
Bridges are critical components of transportation networks, and their conditions have effects on societal well-being, the economy, and the environment. Automation needs in inspections and maintenance have made structural health monitoring (SHM) systems a key research pillar to assess bridge safety/health. The last [...] Read more.
Bridges are critical components of transportation networks, and their conditions have effects on societal well-being, the economy, and the environment. Automation needs in inspections and maintenance have made structural health monitoring (SHM) systems a key research pillar to assess bridge safety/health. The last decade brought a boom in innovative bridge SHM applications with the rise in next-generation smart and mobile technologies. A key advancement within this direction is smartphones with their sensory usage as SHM devices. This focused review reports recent advances in bridge SHM backed by smartphone sensor technologies and provides case studies on bridge SHM applications. The review includes modelbased and data-driven SHM prospects utilizing smartphones as the sensing and acquisition portal and conveys three distinct messages in terms of the technological domain and level of mobility: (i) vibration-based dynamic identification and damage-detection approaches; (ii) deformation and condition monitoring empowered by computer vision-based measurement capabilities; (iii) drive-by or pedestrianized bridge monitoring approaches, and miscellaneous SHM applications with unconventional/emerging technological features and new research domains. The review is intended to bring together bridge engineering, SHM, and sensor technology audiences with decade-long multidisciplinary experience observed within the smartphone-based SHM theme and presents exemplary cases referring to a variety of levels of mobility. Full article
11 pages, 435 KiB  
Article
Outcomes Before and After Implementation of the ERAS (Enhanced Recovery After Surgery) Protocol in Open and Laparoscopic Colorectal Surgery: A Comparative Real-World Study from Northern Italy
by Lucia Mangone, Federica Mereu, Maurizio Zizzo, Andrea Morini, Magda Zanelli, Francesco Marinelli, Isabella Bisceglia, Maria Barbara Braghiroli, Fortunato Morabito, Antonino Neri and Massimiliano Fabozzi
Curr. Oncol. 2024, 31(6), 2907-2917; https://doi.org/10.3390/curroncol31060222 (registering DOI) - 21 May 2024
Abstract
Enhanced Recovery After Surgery (ERAS) protocols have changed perioperative care, aiming to optimize patient outcomes. This study assesses ERAS implementation effects on postoperative complications, length of hospital stay (LOS), and mortality in colorectal cancer (CRC) patients. A retrospective real-world analysis was conducted on [...] Read more.
Enhanced Recovery After Surgery (ERAS) protocols have changed perioperative care, aiming to optimize patient outcomes. This study assesses ERAS implementation effects on postoperative complications, length of hospital stay (LOS), and mortality in colorectal cancer (CRC) patients. A retrospective real-world analysis was conducted on CRC patients undergoing surgery within a Northern Italian Cancer Registry. Outcomes including complications, re-surgeries, 30-day readmission, mortality, and LOS were assessed in 2023, the year of ERAS protocol adoption, and compared with data from 2022. A total of 158 surgeries were performed, 77 cases in 2022 and 81 in 2023. In 2023, a lower incidence of postoperative complications was observed compared to that in 2022 (17.3% vs. 22.1%), despite treating a higher proportion of patients with unfavorable prognoses. However, rates of reoperations and readmissions within 30 days post-surgery increased in 2023. Mortality within 30 days remained consistent between the two groups. Patients diagnosed in 2023 experienced a statistically significant reduction in LOS compared to those in 2022 (mean: 5 vs. 8.1 days). ERAS protocols in CRC surgery yield reduced postoperative complications and shorter hospital stays, even in complex cases. Our study emphasizes ERAS’ role in enhancing surgical outcomes and recovery. Full article
11 pages, 16997 KiB  
Article
A Docking and Network Pharmacology Study on the Molecular Mechanisms of Curcumin in Dental Caries and Streptococcus mutans
by Juan Manuel Guzmán-Flores, Ángel Pérez-Reyes, Sonia Isela Vázquez-Jiménez, Mario Alberto Isiordia-Espinoza and Fernando Martínez-Esquivias
Dent. J. 2024, 12(6), 153; https://doi.org/10.3390/dj12060153 (registering DOI) - 21 May 2024
Abstract
Background: Dental caries is a dynamic, multifactorial disease that destroys teeth and can affect anyone’s quality of life because it can cause tooth loss and make chewing difficult. Dental caries involves various factors, such as Streptococcus mutans and host factors. Currently, adjuvant therapies, [...] Read more.
Background: Dental caries is a dynamic, multifactorial disease that destroys teeth and can affect anyone’s quality of life because it can cause tooth loss and make chewing difficult. Dental caries involves various factors, such as Streptococcus mutans and host factors. Currently, adjuvant therapies, such as curcumin, have emerged, but how they work has not been adequately described. Therefore, this work aims to identify the molecular mechanism of curcumin in caries and Streptococcus mutans. Methods: We obtained differentially expressed genes from a GEO dataset, and curcumin targets were obtained from other databases. The common targets were analyzed according to gene ontology enrichment, key genes were obtained, and binding to curcumin was verified by molecular docking. Results: Our analysis showed that curcumin presents 134 therapeutic targets in caries. According to the gene ontology analysis, these targets are mainly involved in apoptosis and inflammation. There are seven key proteins involved in the action of curcumin on caries: MAPK1, BCL2, KRAS, CXCL8, TGFB1, MMP9, and IL1B, all of which spontaneously bind curcumin. In addition, curcumin affects metabolic pathways related to lipid, purine, and pyrimidine metabolism in Streptococcus mutans. Conclusions: Curcumin affects both host carious processes and Streptococcus mutans. Full article
(This article belongs to the Special Issue Updates and Highlights in Cariology)
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11 pages, 851 KiB  
Article
Primitive Chain Network Simulations for Double Peaks in Shear Stress under Fast Flows of Bidisperse Entangled Polymers
by Yuichi Masubuchi
Polymers 2024, 16(11), 1455; https://doi.org/10.3390/polym16111455 (registering DOI) - 21 May 2024
Abstract
A few experiments have reported that the time development of shear stress under fast-startup shear deformations exhibits double peaks before reaching a steady state for bimodal blends of entangled linear polymers under specific conditions. To understand this phenomenon, multi-chain slip-link simulations, based on [...] Read more.
A few experiments have reported that the time development of shear stress under fast-startup shear deformations exhibits double peaks before reaching a steady state for bimodal blends of entangled linear polymers under specific conditions. To understand this phenomenon, multi-chain slip-link simulations, based on the primitive chain network model, were conducted on the literature data of a bimodal polystyrene solution. Owing to reasonable agreement between their data and our simulation results, the stress was decomposed into contributions from long- and short-chain components and decoupled into segment number, stretch, and orientation. The analysis revealed that the first and second peaks correspond to the short-chain orientation and the long-chain stretch, respectively. The results also implied that the peak positions are not affected by the mixing of short and long chains, although the intensity of the second peak depends on mixing conditions in a complicated manner. Full article
(This article belongs to the Special Issue Rheological Properties of Polymers and Polymer Composites)
14 pages, 1370 KiB  
Article
Adherence to Mediterranean Diet and Diet Quality in Patients with Inflammatory Bowel Disease: A Single-Center, Observational, Case-Control Study
by Marta Cadoni, Agnese Favale, Rita Piras, Mauro Demurtas, Paola Soddu, Alessandra Usai, Ivan Ibba, Massimo Claudio Fantini and Sara Onali
Nutrients 2024, 16(11), 1557; https://doi.org/10.3390/nu16111557 (registering DOI) - 21 May 2024
Abstract
The nutritional status in inflammatory bowel disease (IBD) is often impaired, and adherence to the Mediterranean diet (MedDiet) remains under-investigated. The aim of this study was to assess diet quality (DQ) and adherence to MedDiet in a cohort of Sardinian IBD patients. We [...] Read more.
The nutritional status in inflammatory bowel disease (IBD) is often impaired, and adherence to the Mediterranean diet (MedDiet) remains under-investigated. The aim of this study was to assess diet quality (DQ) and adherence to MedDiet in a cohort of Sardinian IBD patients. We conducted a case-control study in which 50 Crohn’s disease (CD) and 50 ulcerative colitis (UC) patients were matched with 100 healthy controls each. The Diet Quality Index (DQI-I) and Medi-Lite were used to assess DQ and adherence to MedDiet, respectively. Subgroup analysis by disease characteristics and use of advanced therapies were also carried out. DQI-I scored significantly lower in IBD, independently of disease localization and behavior (CD) and disease extent (UC): [DQI-I: CD 34.5 (IQR 33–37) vs. CTRL 40 (IQR 38.5–43) p < 0.0001; UC 34.5 (IQR 33–37) vs. CTRL 42 (IQR 40–44) p < 0.0001]. Medi-Lite scores were significantly lower in stricturing and ileo-colonic CD and in extensive UC: [Medi-Lite CD 7.5 (IQR 7–9)] vs. CTRL 9 (IQR 7–10) p = 0.0379]; [UC 8 (IQR7–10) vs. CTRL 9 (IQR 8–10.5) p = 0.0046]. IBD patients had a low DQ independently of disease type and phenotype. Patients with ileo-colonic stenosing CD or extensive UC had lower MedDiet adherence, suggesting that its benefits may be mitigated by low acceptance in specific subgroups. Full article
(This article belongs to the Section Clinical Nutrition)
36 pages, 2406 KiB  
Article
Design and Control of an Ultra-Low-Cost Logistic Delivery Fixed-Wing UAV
by Yixuan Zhang, Qinyang Zhao, Peifu Mao, Qiaofeng Bai, Fuzhong Li and Svitlana Pavlova
Appl. Sci. 2024, 14(11), 4358; https://doi.org/10.3390/app14114358 (registering DOI) - 21 May 2024
Abstract
In contemporary logistics, the deployment of fixed-wing unmanned aerial vehicles (UAVs) as a transportation platform is experiencing rapid advancements, garnering substantial application within numerous logistic operations with pronounced efficacies. There are notable impediments to the utilization of commercial logistic-oriented fixed-wing UAVs, including elevated [...] Read more.
In contemporary logistics, the deployment of fixed-wing unmanned aerial vehicles (UAVs) as a transportation platform is experiencing rapid advancements, garnering substantial application within numerous logistic operations with pronounced efficacies. There are notable impediments to the utilization of commercial logistic-oriented fixed-wing UAVs, including elevated procurement and maintenance costs, extensive maintenance intervals, and unsuitability for small-volume, low-altitude transport tasks. These factors collectively exacerbate the risk associated with enterprise procurement and elevate the cost–benefit ratio. This study introduces the design and fabrication of a cost-efficient UAV for logistic delivery purposes, constructed primarily from cost-effective wood materials. This UAV is engineered to ferry payloads of up to 1000 g across a predefined aerial route at an altitude of 40 m. Upon reaching the designated location, the UAV is programmed to initiate the identification of the drop zone, thereafter descending to facilitate the release of the cargo. To mitigate the impact force during the landing phase, the payload was encapsulated within a sponge-damping layer, thereby preserving the integrity of the transported items. The empirical findings from outdoor delivery trials underscore the UAV’s ability to precisely execute payload drops at the targeted locations, confirming its potential to fulfill the logistical requirements for the transportation and delivery of small-volume items in a cost-effective, low-altitude framework. This investigation contributes to the burgeoning discourse on leveraging ultra-low-cost UAVs in logistics, offering a feasible solution to the challenges of cost and efficiency in UAV-operated delivery systems. Full article
14 pages, 1674 KiB  
Article
Repeatable Passive Fiber Optic Coupling of Single-Mode Waveguides in High-Precision Disposable Photonic Biosensors
by Jakob Reck, Laurids von Emden, Klara Mihov, Martin Kresse, Madeleine Weigel, Tianwen Qian, Csongor Keuer, Philipp Winklhofer, Marcel Amberg, David de Felipe, Crispin Zawadzki, Moritz Kleinert, Norbert Keil and Martin Schell
Photonics 2024, 11(6), 488; https://doi.org/10.3390/photonics11060488 (registering DOI) - 21 May 2024
Abstract
This research demonstrates a method for the repeatable passive fiber optic coupling of single-mode waveguides with a micron-scale accuracy for high-precision disposables. The aim is to broaden the application of photonic integrated circuits (PICs) from traditional fiber optic communication systems to include medical, [...] Read more.
This research demonstrates a method for the repeatable passive fiber optic coupling of single-mode waveguides with a micron-scale accuracy for high-precision disposables. The aim is to broaden the application of photonic integrated circuits (PICs) from traditional fiber optic communication systems to include medical, life science, and environmental sensing applications. The proposed passive coupling system enables the straightforward and reliable interchange of disposable photonic chips without manual read-out unit adjustments. Robustness is attributed to the chip-side grating couplers with 3 dB coupling tolerances exceeding ± 25 µm and a mechanical three-groove kinematic method ensuring precise alignment. The system simplicity is highlighted by the simple manual insertion and fixation of silicon nitride (Si3N4) PICs on a carrier using magnetic force and passive alignment features. Testing on a batch of 99 identical yet independent units revealed a standard deviation (SD) of 5.1 dB in coupling loss, without realignment post-calibration. This eliminates the need for active alignment processes, showing its potential for enabling field use. A usability assessment with five untrained operators confirms the suitability for various end-users, with consistent performance in engaging and disengaging disposable PICs. The research significantly advances the integration of photonic sensor technology into practical applications, particularly for chemical and biological fluid analysis in point-of-care settings. Full article
(This article belongs to the Special Issue Advanced Photonic Sensing and Measurement II)
37 pages, 1542 KiB  
Review
Unlocking the Potential of Oleanolic Acid: Integrating Pharmacological Insights and Advancements in Delivery Systems
by Muhammad Wasim and Maria Camilla Bergonzi
Pharmaceutics 2024, 16(6), 692; https://doi.org/10.3390/pharmaceutics16060692 (registering DOI) - 21 May 2024
Abstract
The growing interest in oleanolic acid (OA) as a triterpenoid with remarkable health benefits prompts an emphasis on its efficient use in pharmaceutical research. OA exhibits a range of pharmacological effects, including antidiabetic, anti-inflammatory, immune-enhancing, gastroprotective, hepatoprotective, antitumor, and antiviral properties. While OA [...] Read more.
The growing interest in oleanolic acid (OA) as a triterpenoid with remarkable health benefits prompts an emphasis on its efficient use in pharmaceutical research. OA exhibits a range of pharmacological effects, including antidiabetic, anti-inflammatory, immune-enhancing, gastroprotective, hepatoprotective, antitumor, and antiviral properties. While OA demonstrates diverse pharmacological effects, optimizing its therapeutic potential requires overcoming significant challenges. In the field of pharmaceutical research, the exploration of efficient drug delivery systems is essential to maximizing the therapeutic potential of bioactive compounds. Efficiently delivering OA faces challenges, such as poor aqueous solubility and restricted bioavailability, and to unlock its full therapeutic efficacy, novel formulation strategies are imperative. This discussion thoroughly investigates different approaches and advancements in OA drug delivery systems with the aim of enhancing the biopharmaceutical features and overall efficacy in diverse therapeutic contexts. Full article
(This article belongs to the Special Issue Novel Drug Delivery Systems of Phytomedicines)
13 pages, 3010 KiB  
Article
BiLSTM- and CNN-Based m6A Modification Prediction Model for circRNAs
by Yuqian Yuan, Xiaozhu Tang, Hongyan Li, Xufeng Lang, Yihua Song, Ye Yang and Zuojian Zhou
Molecules 2024, 29(11), 2429; https://doi.org/10.3390/molecules29112429 (registering DOI) - 21 May 2024
Abstract
m6A methylation, a ubiquitous modification on circRNAs, exerts a profound influence on RNA function, intracellular behavior, and diverse biological processes, including disease development. While prediction algorithms exist for mRNA m6A modifications, a critical gap remains in the prediction of circRNA m6A modifications. Therefore, [...] Read more.
m6A methylation, a ubiquitous modification on circRNAs, exerts a profound influence on RNA function, intracellular behavior, and diverse biological processes, including disease development. While prediction algorithms exist for mRNA m6A modifications, a critical gap remains in the prediction of circRNA m6A modifications. Therefore, accurate identification and prediction of m6A sites are imperative for understanding RNA function and regulation. This study presents a novel hybrid model combining a convolutional neural network (CNN) and a bidirectional long short-term memory network (BiLSTM) for precise m6A methylation site prediction in circular RNAs (circRNAs) based on data from HEK293 cells. This model exploits the synergy between CNN’s ability to extract intricate sequence features and BiLSTM’s strength in capturing long-range dependencies. Furthermore, the integrated attention mechanism empowers the model to pinpoint critical biological information for studying circRNA m6A methylation. Our model, exhibiting over 78% prediction accuracy on independent datasets, offers not only a valuable tool for scientific research but also a strong foundation for future biomedical applications. This work not only furthers our understanding of gene expression regulation but also opens new avenues for the exploration of circRNA methylation in biological research. Full article
15 pages, 1177 KiB  
Article
Identification Method of Crop Aphids Based on Bionic Attention
by Yue Hu, Zhiyong Li, Zhengyang Lu, Xinyu Jia, Pengsen Wang and Xulin Liu
Agronomy 2024, 14(6), 1093; https://doi.org/10.3390/agronomy14061093 (registering DOI) - 21 May 2024
Abstract
The accurate identification of crop aphids is an important aspect of improving agricultural productivity. Aphids are characterised by small targets and a body colour similar to their surroundings. Even the most advanced detectors can experience problems such as low detection accuracy and a [...] Read more.
The accurate identification of crop aphids is an important aspect of improving agricultural productivity. Aphids are characterised by small targets and a body colour similar to their surroundings. Even the most advanced detectors can experience problems such as low detection accuracy and a high number of missed detections. In this paper, a multi-stream target detection model is proposed for fast and accurate detection of crop aphids in complex backgrounds. First, inspired by the human visual system, we propose a bionic attention (BA) approach. Unlike previous strategies, we do not improve the model but input additional category labels as bionic information streams into the network at the network input stage to support mainstream recognition, which improves the detection effect. In addition, through web crawling and manual screening, we construct an aphid dataset containing 2059 images, named IP_Aphids, based on IP102. In this paper, we combine the proposed BA with a number of classical target detection models, including YOLOv5s, YOLOv7-tiny, YOLOv8n, SSD, and faster R-CNN, and we also compare the BA with the classical attention mechanisms CBAM and SE. This approach allows the effectiveness of the method to be evaluated from multiple perspectives. The results show that combining the bionic information flow of BA with the original mainstream information flow as inputs to the five target detection models improves the mAP by 2.2%, 7%, 2.7%, 1.78%, and 1.12%, respectively, compared to the models using only the original inputs. In addition, the mAP of the YOLOv8n_BA model is higher than that of the YOLOv8n, YOLOv8n_CBAM, and YOLOv8n_SE models by 4.6%, 3.3%, and 2.7%, respectively. This indicates that the BA proposed in this study is significantly better than the classical attention to improve crop aphid detection, which provides a reference for crop aphid-related research. Full article
(This article belongs to the Section Precision and Digital Agriculture)
21 pages, 1017 KiB  
Review
Implications of Prenatal Exposure to Endocrine-Disrupting Chemicals in Offspring Development: A Narrative Review
by Juan M. Toledano, Maria Puche-Juarez, Jorge Moreno-Fernandez, Patricia Gonzalez-Palacios, Ana Rivas, Julio J. Ochoa and Javier Diaz-Castro
Nutrients 2024, 16(11), 1556; https://doi.org/10.3390/nu16111556 (registering DOI) - 21 May 2024
Abstract
During the last decades, endocrine-disrupting chemicals (EDCs) have attracted the attention of the scientific community, as a result of a deepened understanding of their effects on human health. These compounds, which can reach populations through the food chain and a number of daily [...] Read more.
During the last decades, endocrine-disrupting chemicals (EDCs) have attracted the attention of the scientific community, as a result of a deepened understanding of their effects on human health. These compounds, which can reach populations through the food chain and a number of daily life products, are known to modify the activity of the endocrine system. Regarding vulnerable groups like pregnant mothers, the potential damage they can cause increases their importance, since it is the health of two lives that is at risk. EDCs can affect the gestation process, altering fetal development, and eventually inducing the appearance of many disorders in their childhood and/or adulthood. Because of this, several of these substances have been studied to clarify the influence of their prenatal exposure on the cognitive and psychomotor development of the newborn, together with the appearance of non-communicable diseases and other disorders. The most novel research on the subject has been gathered in this narrative review, with the aim of clarifying the current knowledge on the subject. EDCs have shown, through different studies involving both animal and human investigation, a detrimental effect on the development of children exposed to the during pregnancy, sometimes with sex-specific outcomes. However, some other studies have failed to find these associations, which highlights the need for deeper and more rigorous research, that will provide an even more solid foundation for the establishment of policies against the extended use of these chemicals. Full article
(This article belongs to the Section Nutrition and Public Health)
26 pages, 3766 KiB  
Article
Research on Lateral Resistance Performance of Prestressed Cross-Laminated Timber–Concrete Composite Structures under Reciprocating Loads
by Yong Xu, Xin Huang, Yingda Zhang, Yusen Qu, Yujie Fan and Guoqin Yang
Materials 2024, 17(11), 2485; https://doi.org/10.3390/ma17112485 (registering DOI) - 21 May 2024
Abstract
Cross-Laminated Timber (CLT) and concrete composite structures represent an architectural system that integrates the strengths of both materials. In this innovative configuration, the CLT and concrete collaborate synergistically, harnessing their individual merits to achieve enhanced structural performance and functionality. Specifically, the CLT offers [...] Read more.
Cross-Laminated Timber (CLT) and concrete composite structures represent an architectural system that integrates the strengths of both materials. In this innovative configuration, the CLT and concrete collaborate synergistically, harnessing their individual merits to achieve enhanced structural performance and functionality. Specifically, the CLT offers a lightweight design, superior bending resistance, and immense engineering plasticity, while concrete boasts exceptional compressive strength and durability. This study investigates the mechanical performance of CLT­–concrete composite structures through quasi-static reciprocating loading tests in three full-scale CLT shear wall samples. Designed with varying initial prestressing forces and dimensions of the CLT panel, the prestressed CLT–concrete structures demonstrated a reduced dependence on the steel nodes, resulting in an increase in yield load, yield displacement, and maximum load-carrying capacity. Maximum capacity increased by 39.8% and 33.7% under initial prestressing forces of 23 kN and 46 kN on steel strands. Failure occurred due to localized compressive failure on prestressed steel strands and anchor plates. ABAQUS finite element analysis established three refined models, revealing that the increased initial prestressing force moderately enhanced stiffness but reduced ductility under similar cross-sectional dimensions. Furthermore, under consistent CLT material, dimensions, prestressing force, and loading conditions, prestressed CLT–concrete structures exhibited a higher maximum load-bearing capacity than prestressed CLT–steel composite structures. This study proposes structural design recommendations based on experimental and simulation results, incorporating specific assumptions. Full article
12 pages, 5217 KiB  
Article
Effect of Al Content on Microstructure and Properties of AlxCr0.2NbTiV Refractory High-Entropy Alloys
by Rongbin Li, Qianqian Li, Zhixi Zhang, Rulin Zhang, Yue Xing and Doudou Han
Entropy 2024, 26(6), 435; https://doi.org/10.3390/e26060435 (registering DOI) - 21 May 2024
Abstract
High-temperature creep refers to the slow and continuous plastic deformation of materials under the effects of high temperatures and mechanical stress over extended periods, which can lead to the degradation or even failure of the components’ functionality. AlxCr0.2NbTiV (x [...] Read more.
High-temperature creep refers to the slow and continuous plastic deformation of materials under the effects of high temperatures and mechanical stress over extended periods, which can lead to the degradation or even failure of the components’ functionality. AlxCr0.2NbTiV (x = 0.2, 0.5, or 0.8) refractory high-entropy alloys were fabricated by arc melting. The effects of Al content on the microstructure of AlxCr0.2NbTiV alloys were studied using X-ray diffraction, scanning electron microscopy, and electron backscatter diffraction. The microhardness, compression properties, and nanoindentation creep properties of AlxCr0.2NbTiV alloys were also tested. The results show that the AlxCr0.2NbTiV series exhibits a BCC single-phase structure. As the Al content increases, the lattice constant of the alloys gradually decreases, and the intensity of the (110) crystal plane diffraction peak increases. Adding aluminum enhances the effect of solution strengthening; however, due to grain coarsening, the microhardness and room temperature compressive strength of the alloy are only slightly improved. Additionally, because the effect of solution strengthening is diminished at high temperatures, the compressive strength of the alloy at 1000 °C is significantly reduced. The creep mechanism of the alloys is predominantly governed by dislocation creep. Moreover, increasing the Al content helps to reduce the sensitivity of the alloy to the loading rate during the creep process. At a loading rate of 2.5 mN/s, the Al0.8Cr0.2NbTiV alloy exhibits the lowest creep strain rate sensitivity index (m), which is 0.0758. Full article
(This article belongs to the Special Issue Recent Advances in Refractory High Entropy Alloys)
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23 pages, 2288 KiB  
Article
Research of the Influence of Lateral Inflow Angles on the Cavitation Flow and Movement Characteristics of Underwater Moving Objects
by Rishan Xie, Huixia Jia, Jiawei Chen, Lite Zhang and Chengwei Zhang
Processes 2024, 12(6), 1051; https://doi.org/10.3390/pr12061051 (registering DOI) - 21 May 2024
Abstract
This study examined the multi-phase flow field for a single object and two parallel/series objects under different incoming angles of lateral flow. The volume of fluid model, the Sauer–Schnerr cavitation model, and the six degrees of freedom (DOF) method were adopted to consider [...] Read more.
This study examined the multi-phase flow field for a single object and two parallel/series objects under different incoming angles of lateral flow. The volume of fluid model, the Sauer–Schnerr cavitation model, and the six degrees of freedom (DOF) method were adopted to consider simulations of multi-phase flow, phase change, and object movement, respectively. The results show that, for a single object, the degree of asymmetry in the cavity profile depends on the component (the z-component) of the lateral inflow velocity in the direction perpendicular to the initial velocity of the object. As this component increases, the asymmetry of the cavity increases. The cavity length is related to the relative axial speed between the object and the water. For parallel objects, the cavity asymmetry is determined by the superimposed influence of the z-component of the lateral incoming speed and the high-pressure zone induced by the nearby object. The object located downstream relative to the lateral flow has a stronger cavity asymmetry than that of the upstream object, and the trajectory of the downstream object is more easily deviated than that of the upstream object. For tandem objects, with the increase in the lateral incoming angle, the supercavity length increases after the rear object enters into the front cavity. With the increase in the z-component of the lateral flow velocity, the deviation speed increases. Full article
(This article belongs to the Section Advanced Digital and Other Processes)

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