This paper presents an enhanced ground vehicle localization method designed to address the challenges associated with state estimation for autonomous vehicles operating in diverse environments. The focus is specifically on the precise localization of position and orientation in both local and global coordinate systems. The proposed approach integrates local estimates generated by existing visual–inertial odometry (VIO) methods into global position information obtained from the Global Navigation Satellite System (GNSS). This integration is achieved through optimizing fusion in a pose graph, ensuring precise local estimation and drift-free global position estimation. Considering the inherent complexities in autonomous driving scenarios, such as the potential failures of a visual–inertial navigation system (VINS) and restrictions on GNSS signals in urban canyons, leading to disruptions in localization outcomes, we introduce an adaptive fusion mechanism. This mechanism allows seamless switching between three modes: utilizing only VINS, using only GNSS, and normal fusion. The effectiveness of the proposed algorithm is demonstrated through rigorous testing in the Carla simulation environment and challenging UrbanNav scenarios. The evaluation includes both qualitative and quantitative analyses, revealing that the method exhibits robustness and accuracy. Full article

Mitochondrial DNA (mtDNA) exhibits distinct characteristics distinguishing it from the nuclear genome, necessitating specific analytical methods in genetic studies. This comprehensive review explores the complex role of mtDNA in a variety of genetic studies, including genome-wide, epigenome-wide, and phenome-wide association studies, with a focus on its implications for human traits and diseases. Here, we discuss the structure and gene-encoding properties of mtDNA, along with the influence of environmental factors and epigenetic modifications on its function and variability. Particularly significant are the challenges posed by mtDNA’s high mutation rate, heteroplasmy, and copy number variations, and their impact on disease susceptibility and population genetic analyses. The review also highlights recent advances in methodological approaches that enhance our understanding of mtDNA associations, advocating for refined genetic research techniques that accommodate its complexities. By providing a comprehensive overview of the intricacies of mtDNA, this paper underscores the need for an integrated approach to genetic studies that considers the unique properties of mitochondrial genetics. Our findings aim to inform future research and encourage the development of innovative methodologies to better interpret the broad implications of mtDNA in human health and disease. Full article

The study focuses on the four-strand tundish as the research object, aiming at the phenomenon of fewer strand casting (stable blockage) and sudden blockage of the tundish in industrial production. Numerical simulation methods are employed to compare the velocity vectors, flow fields, residence time distribution (RTD) curves, and outflow percentage curves under stable blockage and sudden blockage of the tundishes with a double-weir structure, U-shaped weir structure, and U-shaped weir structure with holes in the front. The results indicate that, after sudden blockage of the tundish strands, the flow field transitions from an unstable four-strand flow field to a stable three-strand flow field. Both the double-weir tundish and the U-shaped weir tundish reach a stable state after 200 s, while the U-shaped weir tundish with holes in the front reaches stability after 150 s. Additionally, compared to other structures, the tundish strands of the U-shaped weir with holes in the front are less affected by blockage, showing better consistency among strands and better adaptability under non-standard casting conditions. Full article

Concrete prepared using Gobi sand and gravel instead of ordinary sand and gravel is referred to as Gobi concrete. In order to explore the effect of fibers on the frost resistance of Gobi concrete, as well as to enhance the service life of Gobi aggregate concrete in Northwest China, experiments were conducted with fiber types (polypropylene fibers, basalt fibers, polypropylene–basalt fibers) and fiber volume fractions (0%, 0.1%, 0.2%, 0.3%) as variable parameters. This study investigated the surface morphology, mass loss rate, and relative dynamic elastic modulus of fiber-reinforced Gobi concrete after different freeze–thaw cycles (0, 25, 50, 75, 100). Corresponding frost damage deterioration models were proposed. The results indicate that fibers have a favorable effect on the anti-peeling performance, mass loss rate, and dynamic elastic modulus of Gobi aggregate concrete. The improvement levels of different fiber types are in the following order: 0.1% basalt-polypropylene fibers, 0.2% polypropylene fibers, and 0.3% basalt fibers. Compared to Gobi concrete exposed to natural environmental conditions, the freeze–thaw cycle numbers increased by 343, 79, and 69 times, respectively. A quadratic polynomial damage model for fiber-reinforced Gobi concrete, using relative dynamic elastic modulus as the damage variable, was established and demonstrated good predictive performance. Full article

Tourism is one of the emerging branches of the economy, playing an important role in the development of specific economies within local communities. In this context, the perspectives of exploiting historical monuments, seen as raw material in the tourism industry, represent a desirable goal worth considering at the locality and territorial administrative unit level. The purpose of this study is to highlight the relationship between historical monuments, viewed as factors generating tourist motivation and tourism. This was made possible by conducting a spatial analysis (at the level of territorial administrative units and localities) of the defining criteria for historical monuments and tourism in Bihor County, Romania. The research methodology involved the use of multicriteria analysis to identify and establish the types of relationships between historical monuments and tourism, at a spatial level. The results of the study aimed to present an image of the spatial distribution of the characteristics of historical monuments and tourism, as well as to establish and depict spatial relationships between them, thus partially confirming the working hypothesis that the number and importance of historical monuments influence and determine tourist activity within a given area. Thus, although the studied area has 455 historical monuments, they are not exploited from a tourist point of view, with there being no strong relationships, except at the level of 19 territorial administrative units (18.8%), respectively, in 15 localities (3.3%). Among them, the obtained values stand out for the territorial administrative units of Oradea and Biharia, respectively, in the localities of Oradea and Beiuș. Full article

Acute pneumonia (AP), triggered primarily by pathogens like bacteria and viruses, is a leading cause of human mortality. Ribavirin, a broad-spectrum antiviral agent, plays a pivotal role in the treatment of AP. However, its therapeutic use is hindered by the need for high dosages and the associated cardiac and hepatic toxicities. In this study, we synthesized polyethylene glycol-modified cationic liposomes to encapsulate ribavirin (RBV-PCL) and formulated it into a spray, aiming to enhance the effectiveness of RBV through respiratory administration. Lipopolysaccharide (LPS), a compound known to induce AP models in animals, was utilized in our research. Successfully, we established an acute pneumonia model in mice using aerosol inhalation. Through animal experiments, we investigated the therapeutic effects of RBV-PCL on mice with AP. In vivo studies revealed promising results. RBV-PCL effectively prolonged the survival of mice with AP, significantly reduced the levels of inflammatory markers such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), and inhibited the infiltration of neutrophils in the lungs and spleens of mice. These findings suggest that RBV-PCL can effectively suppress the inflammatory response in mice with AP, thus holding significant potential as a novel therapeutic approach for the treatment of acute pneumonia. Full article

This paper presents a methodology for generating virtual ground control points (VGCPs) using a binocular camera mounted on a drone. We compare the measurements of the binocular and monocular cameras between the classical method and the proposed one. This work aims to decrease human processing times while maintaining a reduced root mean square error (RMSE) for 3D reconstruction. Additionally, we propose utilizing COLMAP to enhance reconstruction accuracy by solely utilizing a sparse point cloud. The results demonstrate that implementing COLMAP for pre-processing reduces the RMSE by up to 16.9% in most cases. We prove that VGCPs further reduce the RMSE by up to 61.08%. Full article

Background: Patients who were infected with COVID-19 may experience Long COVID syndrome. We examined the effectiveness of physiotherapy on dyspnea, muscle strength, and functional status in Long COVID syndrome. Methods: The exercise group underwent an 8-week supervised physiotherapeutic program consisting of interval aerobic exercise and strengthening exercises, each lasting 30 min. The control group did not engage in any exercise. Dyspnea, muscle strength, and functional status were assessed at the beginning and end of the intervention in both groups. Results: No significant baseline differences were found between the two groups. The exercise group demonstrated improvements compared to baseline in dyspnea, quadriceps muscle strength, and functional status. Specifically, there was a significant increase of 3.7 lifts in the 60-s sit-to-stand test (p = 0.01), an increase of 5.86 kg in right quadriceps muscle strength (p = 0.03), an increase of 8.26 kg in left quadriceps muscle strength (p = 0.01), and a decrease in dyspnea score by 0.95 points (p = 0.02). Conclusions: Similar studies have reported improvements in dyspnea, muscle strength, and functional status in the exercise group. However, further research with larger sample sizes is needed to confirm these findings. Full article

Anti-interferon (IFN)-γ autoantibodies are linked to varicella zoster virus (VZV) infection. Given the elevated risks of herpes zoster (HZ) in rheumatoid arthritis (RA) patients treated with Janus kinase inhibitors (JAKis), we aimed to examine the relationship between anti-IFN-γ autoantibodies with HZ development in JAKi-treated patients. Serum titers of anti-IFN-γ autoantibodies, plasma levels of IFN-γ, monocyte chemoattractant protein-1 (MCP-1), and IFN-γ-inducible protein-10 (IP-10) were measured by ELISA. Among the 66 enrolled RA patients, 24 developed new-onset HZ. Significantly lower MCP-1 levels were observed in patients with HZ compared to those without (median, 98.21 pg/mL, interquartile range (IQR) 77.63–150.30 pg/mL versus 142.3 pg/mL, IQR 106.7–175.6 pg/mL, p < 0.05). There was no significant difference in anti-IFN-γ titers, IFN-γ levels, or IP-10 levels between patients with and without HZ. Three of 24 patients with HZ had severe HZ with multi-dermatomal involvement. Anti-IFN-γ titers were significantly higher in patients with severe HZ than in those with non-severe HZ (median 24.8 ng/mL, IQR 21.0–38.2 ng/mL versus 10.5 ng/mL, IQR 9.9–15.0 ng/mL, p < 0.005). Our results suggest an association between reduced MCP-1 levels and HZ development in JAKi-treated RA patients. High-titer anti-IFN-γ autoantibodies may be related to severe HZ in these patients. Full article

Electroplating nickel-63, a radioactive isotope used in betavoltaic batteries and random number generators, requires precise control due to its limited availability and the generation of radioactive waste. To minimize waste and ensure effective plating, small plating baths are employed, optimizing the process within constrained conditions. X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) were utilized to determine the optimal plating conditions and limiting conditions for nickel electroplating in a small plating bath. This study focuses on the use of low-concentration nickel solutions and small plating equipment, in contrast to the common industrial practice of using high concentrations of nickel. Here, it is important to optimize the plating parameters, especially the nickel concentration, current density, and bath temperature. An average thickness of 1.8 μm was found when plating with a nickel concentration of 0.06 M, a current density of 5 mA/cm², and a solution temperature of 40 °C, while ideal conditions were found to achieve the theoretical maximum energy and 90% release rate when plating with nickel-63 instead of Ni. Full article

The low-permeability argillaceous rock mass is an unfavorable geological body commonly found in the construction process of underground engineering conditions such as roadways and tunnels. Due to the compact structure and low permeability of the rock mass, grouting with conventional materials cannot effectively seal the micro-cracks of the rock mass. Based on the low efficiency of high-pressure grouting of nano-silica sol, this paper preliminarily explores the regularities and mechanism of grouting and pore sealing of low-permeability rock mass under the action of silica sol imbibition from the aspects of gelling properties of silica sol, core pore structure, imbibition law, and pore sealing characteristics. The results show the following: (1) The increase in particle size during the gel process reduced the injectability and wettability of the silica sol. The imbibition properties of silica sol were time-varying, and the deterioration inflection points of injectability and wettability appeared at 10 h and 9 h, respectively. (2) Catalyst, temperature, gel process, and rock mass permeability will affect the law of core imbibition, and the injectability and capillary force of the grouting material and rock mass will jointly affect the imbibition process of silica sol. (3) Silica sol imbibition changed the pore size distribution of the core, the pore volume above 50 nm decreased, and the pore volume below 50 nm increased. Silica sol has multiple effects such as filling, adsorption, and percolation in the imbibition process of the micro-pores of rock mass, and the adsorption and percolation of silica are related to the nano micro-pores. Full article

Targeted therapies with chemotherapeutic agents and immunotherapy with checkpoint inhibitors are among the systemic therapies recommended in the guidelines for clinicians to treat melanoma. Although there have been constant improvements in the treatment of melanoma, resistance to the established therapies continues to occur. Therefore, the purpose of this study was to explore the function of garcinol with regards to specific cancer properties such as proliferation and apoptosis. Garcinol, a natural compound isolated from the plant also known as mangosteen (Garcinia mangostana), is a newly discovered option for cancer treatment. Numerous pharmaceutical substances are derived from plants. For example, the derivates of camptothecin, extracted from the bark of the Chinese tree of happiness (Camptotheca acuminate), or paclitaxel, extracted from the bark of the Western yew tree (Taxus brevifolia), are used as anti-cancer drugs. Here, we show that garcinol reduced proliferation and induced apoptosis in melanoma cell lines. In addition, we found that those cells that are positive for the expression of the cell–cell adhesion molecule T-cadherin (CDH13) respond more sensitively to treatment with garcinol. After knock-down experiments with an siRNA pool against T-cadherin, the sensitivity to garcinol decreased and proliferation and anti-apoptotic behavior of the cells was restored. We conclude that patients who are T-cadherin-positive could especially benefit from a therapy with garcinol. Full article

This research paper investigates an LED strip lighting system, whose LED lighting controller was developed based on the PIC24FV32KA302 microcontroller and PCA9685 PWM driver. During the study, various parameters of the system were evaluated, including the response time from the length of commands, the dependence of the PWM signal characteristics on the logic values generated by the microcontroller, and the dependence of the LED light flux and power on the percentage of the PWM signal and the power of the power supply channels. The results of the study revealed that the reaction time depended on the length of the sent command, where as the logic values of the microcontroller were changed from 1000 to 4000, the size of the PWM signal changed from 25 to 100%. The use of the I2C communication protocol, which is a master–slave architecture and uses data and synchronization lines, was also found to affect response times. When the percentage of the PWM signal was changed from 10 to 100%, the light flux of the LED strip changed from 100 to 1000 lm. These results reflect the advantages of applying microcontrollers and PWM drivers to LED control systems, emphasizing their flexibility, efficiency, and precise light control. Full article

Image dehazing aims to reconstruct potentially clear images from corresponding images corrupted by haze. With the rapid development of deep learning-related technologies, dehazing methods based on deep convolutional neural networks have gradually become mainstream. We note that existing dehazing methods often accompany an increase in computational overhead while improving the performance of dehazing. We propose a novel lightweight dehazing neural network to balance performance and efficiency: the g2D-Net. The g2D-Net borrows the design ideas of input-adaptive and long-range information interaction from Vision Transformers and introduces two kinds of convolutional blocks, i.e., the g2D Block and the FFT-g2D Block. Specifically, the g2D Block is a residual block with second-order gated units, which inherit the input-adaptive property of a gated unit and can realize the second-order interaction of spatial information. The FFT-g2D Block is a variant of the g2D Block, which efficiently extracts the global features of the feature maps through fast Fourier convolution and fuses them with local features. In addition, we employ the SK Fusion layer to improve the cascade fusion layer in a traditional U-Net, thus introducing the channel attention mechanism and dynamically fusing information from different paths. We conducted comparative experiments on five benchmark datasets, and the results demonstrate that the g2D-Net achieves impressive dehazing performance with relatively low complexity. Full article

This study explores how lighting can be employed as a visual language to enhance communication between the space and its users and develop a design framework for educational spaces. A primary school is used as a case study to apply the proposed design framework. The study focuses on lighting interventions in existing educational spaces to support daily schedules and transitions between activities. In this context, electric light is used as an indicator, highlighting the daily schedule and activities in the space. A theoretical approach is used as a foundation for establishing the design framework that leads to lighting proposals based on the specific spatial characteristics of each study. The outcome is a design solution based on the dominant spatial elements that define the space’s identity and function. The study focuses on educational spaces and lighting for peripheral vision while considering pupils’ visual and spatial development. The proposal has the role of an additional light layer that signals transitions in terms of activities or spatial mobility. Full article

The retreat of mountain glaciers inevitably leads to an increase in the number of outburst moraine lakes. One of the possible mechanisms of moraine dam outburst along with overflow over the crest is the formation of a filtration channel in the body of the moraine dam (piping). An algorithm for calculating the outburst flood hydrograph, describing the development of a filtration channel in the body of a moraine dam and the subsequent formation of water overflow when the soil above the channel collapses, is proposed in this paper. Verification of proposed methodology was carried out on the basis of experimental data and published data of real outbursts. Satisfactory results verifying this methodology made it possible to use the proposed methodology for the calculation of the hydrograph of the outburst of Lake Bashkara in the Elbrus region, which occurred on 1 September 2017. It is shown that the simulation results are quantitatively comparable with the estimates obtained from field data: the time of water discharge through the channel was 16 min, the period of the outburst wave passage was 40 min, and the maximum discharge was 636 m³/s. Thus, the possibility of applying the proposed methodology for calculating the destruction of natural moraine dams has been demonstrated. Full article

Small-animal models and reverse genetics systems are powerful tools for investigating the molecular mechanisms underlying viral replication, virulence, and interaction with the host immune response in vivo. Rotavirus (RV) causes acute gastroenteritis in many young animals and infants worldwide. Murine RV replicates efficiently in the intestines of inoculated suckling pups, causing diarrhea, and spreads efficiently to uninoculated littermates. Because RVs derived from human and other non-mouse animal species do not replicate efficiently in mice, murine RVs are uniquely useful in probing the viral and host determinants of efficient replication and pathogenesis in a species-matched mouse model. Previously, we established an optimized reverse genetics protocol for RV and successfully generated a murine-like RV rD6/2-2g strain that replicates well in both cultured cell lines and in the intestines of inoculated pups. However, rD6/2-2g possesses three out of eleven gene segments derived from simian RV strains, and these three heterologous segments may attenuate viral pathogenicity in vivo. Here, we rescued the first recombinant RV with all 11 gene segments of murine RV origin. Using this virus as a genetic background, we generated a panel of recombinant murine RVs with either N-terminal VP8* or C-terminal VP5* regions chimerized between a cell-culture-adapted murine ETD strain and a non-tissue-culture-adapted murine EW strain and compared the diarrhea rate and fecal RV shedding in pups. The recombinant viruses with VP5* domains derived from the murine EW strain showed slightly more fecal shedding than those with VP5* domains from the ETD strain. The newly characterized full-genome murine RV will be a useful tool for dissecting virus–host interactions and for studying the mechanism of pathogenesis in neonatal mice. Full article

A low-energy hit, such as a slight fall from a bed, results in a bone fracture, especially in the hip, which is a life-threatening risk for the older adult and a heavy burden for the social economy. Patients with low-energy traumatic bone fractures usually suffer a higher level of bony catabolism accompanied by osteoporosis. Bone marrow-derived stem cells (BMSCs) are critical in osteogenesis, leading to metabolic homeostasis in the healthy bony microenvironment. However, whether the BMSCs derived from the patients who suffered osteoporosis and low-energy traumatic hip fractures preserve a sustained mesodermal differentiation capability, especially in osteogenesis, is yet to be explored in a clinical setting. Therefore, we aimed to collect BMSCs from clinical hip fracture patients with osteoporosis, followed by osteogenic differentiation comparison with BMSCs from healthy young donors. The CD markers identification, cytokines examination, and adipogenic differentiation were also evaluated. The data reveal that BMSCs collected from elderly osteoporotic patients secreted approximately 122.8 pg/mL interleukin 6 (IL-6) and 180.6 pg/mL vascular endothelial growth factor (VEGF), but no PDGF-BB, IL-1b, TGF-b1, IGF-1, or TNF-α secretion. The CD markers and osteogenic and adipogenic differentiation capability in BMSCs from these elderly osteoporotic patients and healthy young donors are equivalent and compliant with the standards defined by the International Society of Cell Therapy (ISCT). Collectively, our data suggest that the elderly osteoporotic patients-derived BMSCs hold equivalent differentiation and proliferation capability and intact surface markers identical to BMSCs collected from healthy youth and are available for clinical cell therapy. Full article

Bisphenol A (BPA), representing a class of organic pollutants, finds extensive applications in the pharmaceutical industry. However, its widespread use poses a significant hazard to both ecosystem integrity and human health. Advanced oxidation processes (AOPs) based on peroxymonosulfate (PMS) via heterogeneous catalysts are frequently proposed for treating persistent pollutants. In this study, the degradation performance of BPA in an oxidation system of PMS activated by transition metal sites anchored nitrogen-doped carbonaceous substrate (M-N-C) materials was investigated. As heterogeneous catalysts targeting the activation of peroxymonosulfate (PMS), M-N-C materials emerge as promising contenders poised to overcome the limitations encountered with traditional carbon materials, which often exhibit insufficient activity in the PMS activation process. Nevertheless, the amalgamation of metal sites during the synthesis process presents a formidable challenge to the structural design of M-N-C. Herein, employing ZIF-8 as the precursor of carbonaceous support, metal ions can readily penetrate the cage structure of the substrate, and the N-rich linkers serve as effective ligands for anchoring metal cations, thereby overcoming the awkward limitation. The research results of this study indicate BPA in water matrix can be effectively removed in the M-N-C/PMS system, in which the obtained nitrogen-rich ZIF-8-derived Cu-N-C presented excellent activity and stability on the PMS activation, as well as the outstanding resistance towards the variation of environmental factors. Moreover, the biological toxicity of BPA and its degradation intermediates were investigated via the Toxicity Estimation Software Tool (T.E.S.T.) based on the ECOSAR system. Full article

Breast cancer is a leading cause of cancer mortality in women worldwide. Using the Infinium MethylationEPIC BeadChip, we analyzed plasma sample methylation to identify the SRCIN1 gene in breast cancer patients. We assessed SRCIN1-related roles and pathways for their biomarker potential. To verify the methylation status, quantitative methylation-specific PCR (qMSP) was performed on genomic DNA and circulating cell-free DNA samples, and mRNA expression analysis was performed using RT‒qPCR. The results were validated in a Western population; for this analysis, the samples included plasma samples from breast cancer patients from the USA and from The Cancer Genome Atlas (TCGA) cohort. To study the SRCIN1 pathway, we conducted cell viability assays, gene manipulation and RNA sequencing. SRCIN1 hypermethylation was identified in 61.8% of breast cancer tissues from Taiwanese patients, exhibiting specificity to this malignancy. Furthermore, its presence correlated significantly with unfavorable 5-year overall survival outcomes. The levels of methylated SRCIN1 in the blood of patients from Taiwan and the USA correlated with the stage of breast cancer. The proportion of patients with high methylation levels increased from 0% in healthy individuals to 63.6% in Stage 0, 80% in Stage I and 82.6% in Stage II, with a sensitivity of 78.5%, an accuracy of 90.3% and a specificity of 100%. SRCIN1 hypermethylation was significantly correlated with increased SRCIN1 mRNA expression (p < 0.001). Knockdown of SRCIN1 decreased the viability of breast cancer cells. SRCIN1 silencing resulted in the downregulation of ESR1, BCL2 and various cyclin protein expressions. SRCIN1 hypermethylation in the blood may serve as a noninvasive biomarker, facilitating early detection and prognosis evaluation, and SRCIN1-targeted therapies could be used in combination regimens for breast cancer patients. Full article

This study aims to investigate the role of Information and Communication Technologies-enabled Education for Sustainability (ICTeEfS), critical reflection, and transformative teaching and learning beliefs in predicting students’ attitudes about seeking sustainability justice. A total of 1497 students from seven universities in Indonesia (374), Malaysia (426), and Vietnam (697) trialed four new scales measuring (a) knowledge of merging ICT with education for sustainability, (b) critical reflective practice, (c) sustainability justice attitudes, and (d) transformative teaching and learning beliefs. The findings show that the four scales are reliable and could be used in other research on education for sustainability. Differences were observed for gender, year of study, subject of study, ICT skills, and knowledge of education for sustainability. Regression analysis highlighted that sustainability justice is a multidimensional concept composed of several constructs with a specific reference to critical reflection, transformative teaching and learning beliefs. The implications for education, practice and further research are discussed. Full article