The 2023 MDPI Annual Report has
been released!
 
11 pages, 3474 KiB  
Article
Somatostatin Receptor Gene Functions in Growth Regulation in Bivalve Scallop and Clam
by Xiangchao Zhang, Yuli Niu, Can Gao, Lingling Kong, Zujing Yang, Lirong Chang, Xiangfu Kong, Zhenmin Bao and Xiaoli Hu
Int. J. Mol. Sci. 2024, 25(9), 4813; https://doi.org/10.3390/ijms25094813 (registering DOI) - 28 Apr 2024
Abstract
Bivalves hold an important role in marine aquaculture and the identification of growth-related genes in bivalves could contribute to a better understanding of the mechanism governing their growth, which may benefit high-yielding bivalve breeding. Somatostatin receptor (SSTR) is a conserved negative regulator of [...] Read more.
Bivalves hold an important role in marine aquaculture and the identification of growth-related genes in bivalves could contribute to a better understanding of the mechanism governing their growth, which may benefit high-yielding bivalve breeding. Somatostatin receptor (SSTR) is a conserved negative regulator of growth in vertebrates. Although SSTR genes have been identified in invertebrates, their involvement in growth regulation remains unclear. Here, we identified seven SSTRs (PySSTRs) in the Yesso scallop, Patinopecten yessoensis, which is an economically important bivalve cultured in East Asia. Among the three PySSTRs (PySSTR-1, -2, and -3) expressed in adult tissues, PySSTR-1 showed significantly lower expression in fast-growing scallops than in slow-growing scallops. Then, the function of this gene in growth regulation was evaluated in dwarf surf clams (Mulinia lateralis), a potential model bivalve cultured in the lab, via RNA interference (RNAi) through feeding the clams Escherichia coli containing plasmids expressing double-stranded RNAs (dsRNAs) targeting MlSSTR-1. Suppressing the expression of MlSSTR-1, the homolog of PySSTR-1 in M. lateralis, resulted in a significant increase in shell length, shell width, shell height, soft tissue weight, and muscle weight by 20%, 22%, 20%, 79%, and 92%, respectively. A transcriptome analysis indicated that the up-regulated genes after MlSSTR-1 expression inhibition were significantly enriched in the fat digestion and absorption pathway and the insulin pathway. In summary, we systemically identified the SSTR genes in P. yessoensis and revealed the growth-inhibitory role of SSTR-1 in bivalves. This study indicates the conserved function of somatostatin signaling in growth regulation, and ingesting dsRNA-expressing bacteria is a useful way to verify gene function in bivalves. SSTR-1 is a candidate target for gene editing in bivalves to promote growth and could be used in the breeding of fast-growing bivalves. Full article
(This article belongs to the Section Molecular Biology)
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30 pages, 357 KiB  
Article
A New Robust Iterative Scheme Applied in Solving a Fractional Diffusion Model for Oxygen Delivery via a Capillary of Tissues
by Godwin Amechi Okeke, Akanimo Victor Udo, Nadiyah Hussain Alharthi and Rubayyi T. Alqahtani
Mathematics 2024, 12(9), 1339; https://doi.org/10.3390/math12091339 (registering DOI) - 28 Apr 2024
Abstract
In this paper, we constructed a new and robust fixed point iterative scheme called the UO iterative scheme for the approximation of a contraction mapping. The scheme converges strongly to the fixed point of a contraction mapping. A rate of convergence result is [...] Read more.
In this paper, we constructed a new and robust fixed point iterative scheme called the UO iterative scheme for the approximation of a contraction mapping. The scheme converges strongly to the fixed point of a contraction mapping. A rate of convergence result is shown with an example, and our scheme, when compared, converges faster than some existing iterative schemes in the literature. Furthermore, the stability and data dependence results are shown. Our new scheme is applied in the approximation of the solution to the oxygen diffusion model. Finally, our results are applied in the approximation of the solution to the boundary value problems using Green’s functions with an example. Full article
(This article belongs to the Special Issue Variational Inequality and Mathematical Analysis)
Article
Cationic Azobenzenes as Light-Responsive Crosslinkers for Alginate-Based Supramolecular Hydrogels
by Miriam Di Martino, Lucia Sessa, Barbara Panunzi, Rosita Diana, Stefano Piotto and Simona Concilio
Polymers 2024, 16(9), 1233; https://doi.org/10.3390/polym16091233 (registering DOI) - 28 Apr 2024
Abstract
Azobenzene photoswitches are fundamental components in contemporary approaches aimed at light-driven control of intelligent materials. Significant endeavors are directed towards enhancing the light-triggered reactivity of azobenzenes for such applications and obtaining water-soluble molecules able to act as crosslinkers in a hydrogel. Here, we [...] Read more.
Azobenzene photoswitches are fundamental components in contemporary approaches aimed at light-driven control of intelligent materials. Significant endeavors are directed towards enhancing the light-triggered reactivity of azobenzenes for such applications and obtaining water-soluble molecules able to act as crosslinkers in a hydrogel. Here, we report the rational design and the synthesis of azobenzene/alginate photoresponsive hydrogels endowed with fast reversible sol–gel transition. We started with the synthesis of three cationic azobenzenes (AZOs A, B, and C) and then incorporated them in sodium alginate (SA) to obtain photoresponsive supramolecular hydrogels (SMHGs). The photoresponsive properties of the azobenzenes were investigated by UV–Vis and 1H NMR spectroscopy. Upon irradiation with 365 nm UV light, the azobenzenes demonstrated efficient trans-to-cis isomerization, with complete isomerization occurring within seconds. The return to the trans form took several hours, with AZO C exhibiting the fastest return, possibly due to higher trans isomer stability. In the photoresponsive SMHGs, the minimum gelation concentration (MGC) of azobenzenes was determined for different compositions, indicating that small amounts of azobenzenes could induce gel formation, particularly in 5 wt% SA. Upon exposure to 365 nm UV light, the SMHGs exhibited reversible gel–sol transitions, underscoring their photoresponsive nature. This research offers valuable insights into the synthesis and photoresponsive properties of cationic, water-soluble azobenzenes, as well as their potential application in the development of photoresponsive hydrogels. Full article
(This article belongs to the Special Issue New Progress in Polymer Self-Assembly)
15 pages, 603 KiB  
Review
Pesticide: A Contemporary Cultural Object
by Elena Fusar Poli and Michele Filippo Fontefrancesco
Encyclopedia 2024, 4(2), 720-734; https://doi.org/10.3390/encyclopedia4020045 (registering DOI) - 28 Apr 2024
Abstract
The article provides a narrative review of the social and cultural interpretation of pesticides in contemporary society. In so doing, it highlights the main fields of exploration investigated by social scientists concerning the perception and role played by these products among farmers and [...] Read more.
The article provides a narrative review of the social and cultural interpretation of pesticides in contemporary society. In so doing, it highlights the main fields of exploration investigated by social scientists concerning the perception and role played by these products among farmers and consumers. Following the WHO definition, pesticides are chemical compounds used to kill pests, including insects, rodents, fungi, and unwanted plants. They are contemporary cultural artefacts that social sciences explore in synergy with other disciplines to highlight their socio-cultural connections at both local and global levels. Specifically, the use of these products is connected with power relations, which are embedded in and reflect imbalances and inequalities in access to rights and resources, as well as specific articulation of the perception of risk arising from environmental contamination in terms of individual and collective psychophysical health. Furthermore, pesticides fit into various environmental conceptions and multiple local knowledge systems, representing the intersection of different cultural heritages, worldviews, and rationales that make the tradition–modernity dichotomy complex and dynamic. In this respect, therefore, pesticides can be understood not just as mere tools for agricultural practice but as vital windows through which to investigate multiple layers of meaning to support transitions towards sustainable pest management systems, both environmentally and socio-culturally. Full article
(This article belongs to the Section Social Sciences)
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26 pages, 7613 KiB  
Article
Extreme Homogeneous and Heterogeneous Multistability in a Novel 5D Memristor-Based Chaotic System with Hidden Attractors
by Chengwei Dong and Min Yang
Fractal Fract. 2024, 8(5), 266; https://doi.org/10.3390/fractalfract8050266 (registering DOI) - 28 Apr 2024
Abstract
This paper proposes a novel five-dimensional (5D) memristor-based chaotic system by introducing a flux-controlled memristor into a 3D chaotic system with two stable equilibrium points, and increases the dimensionality utilizing the state feedback control method. The newly proposed memristor-based chaotic system has line [...] Read more.
This paper proposes a novel five-dimensional (5D) memristor-based chaotic system by introducing a flux-controlled memristor into a 3D chaotic system with two stable equilibrium points, and increases the dimensionality utilizing the state feedback control method. The newly proposed memristor-based chaotic system has line equilibrium points, so the corresponding attractor belongs to a hidden attractor. By using typical nonlinear analysis tools, the complicated dynamical behaviors of the new system are explored, which reveals many interesting phenomena, including extreme homogeneous and heterogeneous multistabilities, hidden transient state and state transition behavior, and offset-boosting control. Meanwhile, the unstable periodic orbits embedded in the hidden chaotic attractor were calculated by the variational method, and the corresponding pruning rules were summarized. Furthermore, the analog and DSP circuit implementation illustrates the flexibility of the proposed memristic system. Finally, the active synchronization of the memristor-based chaotic system was investigated, demonstrating the important engineering application values of the new system. Full article
13 pages, 301 KiB  
Commentary
Botanic Garden Tourism, Social Value, Health, and Well-Being
by Nicholas Catahan, Michelle Hopwood and Piumie Suraweera
J. Zool. Bot. Gard. 2024, 5(2), 187-199; https://doi.org/10.3390/jzbg5020013 (registering DOI) - 28 Apr 2024
Abstract
Many botanic gardens are flourishing, and many others can learn from those leading the way; at the same time, all can form new allegiances informed by service research. We developed this paper to plant seeds for different stakeholders interested in putting a spotlight [...] Read more.
Many botanic gardens are flourishing, and many others can learn from those leading the way; at the same time, all can form new allegiances informed by service research. We developed this paper to plant seeds for different stakeholders interested in putting a spotlight on botanic garden tourism opportunities. It is in response to a call to action by many stakeholders across the botanic garden sector for greater public engagement, to challenge plant awareness disparity, and to ensure the vitality and viability of the sector. Our commentary considers positive, transformative service making, marketing, management, and development. We recommend holistic, integrated services via ecosystemic thinking and collaborative partnerships across the sector and with non-traditional partnerships in the design of sustainable service ecosystems. It is envisaged that service research will spur on a more responsible, ethical, moral enterprise and sustainable botanic garden tourism with opportunities to drive positive, transformative change in meeting sustainable development goals for the good of plants, people, and planet. Full article
(This article belongs to the Special Issue Challenges and Opportunities in Botanic Garden Tourism)
20 pages, 8458 KiB  
Article
Study of Underwater Large-Target Localization Based on Binocular Camera and Laser Rangefinder
by Wenbo Xu, Xinhui Zheng, Qiyan Tian and Qifeng Zhang
J. Mar. Sci. Eng. 2024, 12(5), 734; https://doi.org/10.3390/jmse12050734 (registering DOI) - 28 Apr 2024
Abstract
Currently, for underwater close-range large-target localization, visual localization techniques fail since large targets completely occupy the camera’s field of view at ultraclose ranges. To address the issue, a multi-stage optical localization method combining a binocular camera and a single-point laser rangefinder is proposed [...] Read more.
Currently, for underwater close-range large-target localization, visual localization techniques fail since large targets completely occupy the camera’s field of view at ultraclose ranges. To address the issue, a multi-stage optical localization method combining a binocular camera and a single-point laser rangefinder is proposed in this paper. The proposed method comprises three parts. First, the imaging model of the underwater camera is modified, and a laser rangefinder is used to further correct the underwater calibration results of the binocular camera. Second, YOLOv8 is applied to recognize the targets to prepare for target localization. Third, extrinsic calibration of the binocular camera and laser rangefinder is performed, and a Kalman filter is employed to fuse the target position information measured by the binocular camera and laser rangefinder. The experimental results show that, compared with using a binocular camera alone, the proposed method can accurately and stably locate the target at close ranges with an average error of only 2.27 cm, without the risk of localization failure, and reduces binocular localization error by 90.57%. Full article
(This article belongs to the Section Ocean Engineering)
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16 pages, 5947 KiB  
Data Descriptor
Stimulated Microcontroller Dataset for New IoT Device Identification Schemes through On-Chip Sensor Monitoring
by Alberto Ramos, Honorio Martín, Carmen Cámara and Pedro Peris-Lopez
Data 2024, 9(5), 62; https://doi.org/10.3390/data9050062 (registering DOI) - 28 Apr 2024
Abstract
Legitimate identification of devices is crucial to ensure the security of present and future IoT ecosystems. In this regard, AI-based systems that exploit intrinsic hardware variations have gained notable relevance. Within this context, on-chip sensors included for monitoring purposes in a wide range [...] Read more.
Legitimate identification of devices is crucial to ensure the security of present and future IoT ecosystems. In this regard, AI-based systems that exploit intrinsic hardware variations have gained notable relevance. Within this context, on-chip sensors included for monitoring purposes in a wide range of SoCs remain almost unexplored, despite their potential as a valuable source of both information and variability. In this work, we introduce and release a dataset comprising data collected from the on-chip temperature and voltage sensors of 20 microcontroller-based boards from the STM32L family. These boards were stimulated with five different algorithms, as workloads to elicit diverse responses. The dataset consists of five acquisitions (1.3 billion readouts) that are spaced over time and were obtained under different configurations using an automated platform. The raw dataset is publicly available, along with metadata and scripts developed to generate pre-processed T–V sequence sets. Finally, a proof of concept consisting of training a simple model is presented to demonstrate the feasibility of the identification system based on these data. Full article
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14 pages, 2068 KiB  
Article
Dynamics Power Quality Cost Assessment Based on a Gradient Descent Method
by Jingyi Zhang, Tongtian Sheng, Pan Gu, Miao Yu, Jiaxin Yan, Jianqun Sun and Shanhe Liu
Energies 2024, 17(9), 2104; https://doi.org/10.3390/en17092104 (registering DOI) - 28 Apr 2024
Abstract
The escalating demand for power load is increasingly prone to triggering power quality (PQ) issues, leading to severe economic losses. Aiming at reducing the economic losses, this paper focuses on the coordinated relationship between PQ and economic costs. Firstly, a multilayer multiple linear [...] Read more.
The escalating demand for power load is increasingly prone to triggering power quality (PQ) issues, leading to severe economic losses. Aiming at reducing the economic losses, this paper focuses on the coordinated relationship between PQ and economic costs. Firstly, a multilayer multiple linear stepwise regression method is employed to screen PQ indicators, identifying harmonic and voltage deviation as the primary influencing factors of PQ. Secondly, a gradient descent optimization algorithm based on the Least Absolute Shrinkage and Selection Operator (LASSO) is proposed, enabling rapid computation of the minimum PQ cost. Finally, through validations of two case studies, the results confirm that the proposed method can rapidly calculate the minimum PQ cost based on real-time load demands, enabling the dynamic adjustment of PQ cost to meet the evolving needs of power system development. Full article
(This article belongs to the Special Issue Intelligent Analysis and Control of Modern Power Systems)
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25 pages, 7560 KiB  
Article
Geochemistry and Petrology of Reservoir and Cap Rocks in Zar-3 Pilot CO2 Storage Complex, SE Czechia
by Juraj Francu, Daniela Ocásková, Petr Pařízek, Jakub Vácha, Miroslav Pereszlényi, Petr Jirman, Vladimír Opletal and Monika Ličbinská
Geosciences 2024, 14(5), 119; https://doi.org/10.3390/geosciences14050119 (registering DOI) - 28 Apr 2024
Abstract
The planned pilot CO2 storage Zar-3 is an oil field with a gas cap in the final production stage in the SE Czech Republic. It is composed of a dolomite Jurassic reservoir sealed by three different formations that differ significantly in lithology. [...] Read more.
The planned pilot CO2 storage Zar-3 is an oil field with a gas cap in the final production stage in the SE Czech Republic. It is composed of a dolomite Jurassic reservoir sealed by three different formations that differ significantly in lithology. Previous studies left open questions on the nature of pore space and connectivity and the quality of the seal in the future CO2 storage complex. Microscopic petrography of the reservoir suggests dolomitisation in shallow water followed by karstification and brecciation with fracture-correct-dominated porosity. The seal horizons have porosity limited to the micro- and nanoscales. The oil consists of significantly biodegraded black oil of Jurassic origin mixed with less biodegraded gasoline-range hydrocarbons. Biomarkers in the caprock bitumens trapped in nanopores show a genetic relationship to the reservoir oil. Gas in the not yet fully depleted gas cap of the field is of thermogenic origin with no contribution of microbial methane. The formation water has total dissolved solids typical of isolated brines not diluted by infiltrated fresh water. The geochemical characteristics of the storage system together with the fact that the initial oil column is about 105 m tall with another 150 m of gas cap suggest that the seals are efficient and the Zar-3 future storage complex is tight and safe. Full article
(This article belongs to the Topic Petroleum Geology and Geochemistry of Sedimentary Basins)
7 pages, 828 KiB  
Article
Risk Stratification with Sarculator and MSKCC in Patients with Primary and Secondary Angiosarcoma
by Yonca Steubing, Nilofar Ahmadi, Flemming Puscz, Alexander Wolff, Jannik Hinzmann, Felix Reinkemeier, Sonja Verena Schmidt, Alexander Sogorski, Maxi Von Glinski, Mustafa Becerikli, Maria Füth, Jessica Zuchowski, Hannah Brüggenhorst, Tom Huyghebaert, Ingo Stricker, Marcus Lehnhardt and Christoph Wallner
Life 2024, 14(5), 569; https://doi.org/10.3390/life14050569 (registering DOI) - 28 Apr 2024
Abstract
Background: Sarculator and Memorial Sloan Kettering Cancer Center (MSKCC) nomograms are freely available risk prediction scores for surgically treated patients with primary sarcomas. Due to the rarity of angiosarcomas, these scores have only been tested on small cohorts of angiosarcoma patients. In neither [...] Read more.
Background: Sarculator and Memorial Sloan Kettering Cancer Center (MSKCC) nomograms are freely available risk prediction scores for surgically treated patients with primary sarcomas. Due to the rarity of angiosarcomas, these scores have only been tested on small cohorts of angiosarcoma patients. In neither the original patient cohort upon which the Sarculator is based nor in subsequent studies was a distinction made between primary and secondary angiosarcomas, as the app is intended to be applied to primary sarcomas. Therefore, the objective of our investigation was to assess whether the Sarculator reveals a difference in prognosis and whether such differentiation aligns with actual clinical data. Patients and Methods: Thirty-one patients with primary or secondary soft tissue angiosarcoma, treated at our Sarcoma Center from 2001 to 2023, were included in the study. Actual survival rates were compared with nomogram-derived data for predicted 5-year survival (Sarculator), as well as 4-, 8- and 12-year sarcoma-specific death probabilities (MSKCC). Harrell’s c-index was utilized to assess predictive validity. Results: In total, 31 patients were analyzed. The actual overall 5-year survival was 22.57% with a predicted 5-year survival rate of 25.97%, and the concordance index was 0.726 for the entire cohort. The concordance index results from MSKCC for angiosarcoma patients were below 0.7 indicating limited predictive accuracy in this cohort, particularly when compared to Sarculator. Summary: Nomogram-based predictive models are valuable tools in clinical practice for rapidly assessing prognosis. They can streamline the decision-making process for adjuvant treatments and improve patient counselling especially in the treatment of rare and complicated tumor entities such as angiosarcomas. Full article
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20 pages, 8346 KiB  
Article
Hybrid Machine Learning Algorithms for Prediction of Failure Modes and Punching Resistance in Slab-Column Connections with Shear Reinforcement
by Huajun Yan, Nan Xie and Dandan Shen
Buildings 2024, 14(5), 1247; https://doi.org/10.3390/buildings14051247 (registering DOI) - 28 Apr 2024
Abstract
This study presents a data-driven model for identifying failure modes (FMs) and predicting the corresponding punching shear resistance of slab-column connections with shear reinforcement. An experimental database that contains 328 test results is used to determine nine input variables based on the punching [...] Read more.
This study presents a data-driven model for identifying failure modes (FMs) and predicting the corresponding punching shear resistance of slab-column connections with shear reinforcement. An experimental database that contains 328 test results is used to determine nine input variables based on the punching shear mechanism. A comparison is conducted between three typical machine learning (ML) approaches: random forest (RF), light gradient boosting machine (LightGBM), extreme gradient boosting (XGBoost) and two hybrid optimized algorithms: grey wolf optimization (GWO) and whale optimization algorithm (WOA). It was found that the XGBoost classifier had the highest accuracy rate, precision, and recall values for FM identification. In testing, WOA-XGBoost has the best accuracy in predicting punching shear resistance, with R2, MAE, and RMSE values of 0.9642, 0.087 MN, and 0.126 MN, respectively. However, a comparison between experimental values and calculated values derived from classical analytical methods clearly demonstrates that existing design codes need to be improved. Additionally, Shapley additive explanations (SHAP) were applied to explain the model’s predictions, with factors categorized according to their impact on failure modes and punching shear resistance. By modifying these parameters, punching resistance can be improved while reducing unpredictable failure. With the proposed hybrid algorithms, it is possible to determine the failure modes and the punching shear resistance of slabs during the preliminary stages of the construction. Full article
(This article belongs to the Section Building Structures)
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20 pages, 2276 KiB  
Article
Multibeam Wideband Transmit Beamforming Using 2D Sparse FIR Trapezoidal Filters
by Nadeeshan Dissanayake, Chamira U. S. Edussooriya, Chamith Wijenayake and Arjuna Madanayake
J. Low Power Electron. Appl. 2024, 14(2), 26; https://doi.org/10.3390/jlpea14020026 (registering DOI) - 28 Apr 2024
Abstract
A low-complexity multibeam wideband transmit beamformer using a 2D sparse FIR filter design capable of multiple beams is proposed as a digital building block for fully digital beamformers. The 2D sparse FIR filter has multiple trapezoid-shaped passbands pertaining to wideband beams aimed at [...] Read more.
A low-complexity multibeam wideband transmit beamformer using a 2D sparse FIR filter design capable of multiple beams is proposed as a digital building block for fully digital beamformers. The 2D sparse FIR filter has multiple trapezoid-shaped passbands pertaining to wideband beams aimed at particular directions. The proposed multibeam digital beamformer drives a uniform linear array of wideband antenna elements to achieve the wideband multibeam transmit-mode signals desired by the communication system. The 2D sparse FIR filter is designed to be optimal in the minimax sense using convex optimization techniques. Full-wave electromagnetic simulations using real antenna models confirm that the proposed wideband transmit beamformer can achieve multibeam transmission in the 1.3–2.8 GHz frequency range, with more than 70% fractional bandwidth. Furthermore, the adoption of the wideband transmit multibeam beamformer leads to a significant reduction in digital arithmetic (computational) complexity compared with previously reported wideband transmit beamformers of similar transfer function type, without deteriorating beam directionality and causing increases in the side-lobe level. The proposed sparse 2D FIR multibeam beamformer architecture is well-suited for both sub-6 GHz (legacy) band transmit beamforming, frequency range three (FR3) beamforming up to 28 GHz, and mmWave operation for emerging 5G/6G applications. Full article
20 pages, 19268 KiB  
Article
Does the State of Scientific Knowledge and Legal Regulations Sufficiently Protect the Environment of River Valleys?
by Monika Konatowska, Adam Młynarczyk, Irmina Maciejewska-Rutkowska and Paweł Rutkowski
Land 2024, 13(5), 584; https://doi.org/10.3390/land13050584 (registering DOI) - 28 Apr 2024
Abstract
The pressure of human activity in river valley environments has always been high. Even today, despite the increasing awareness of societies around the world regarding the need to protect water and biodiversity, there are concerns that the current river valley management systems are [...] Read more.
The pressure of human activity in river valley environments has always been high. Even today, despite the increasing awareness of societies around the world regarding the need to protect water and biodiversity, there are concerns that the current river valley management systems are insufficient. Therefore, the aim of this study was to assess the state of knowledge about the soils and forest ecosystems of river valleys in terms of the possibility of protecting river valley environments. This study used data obtained from the Forest Data Bank (FDB) database, which focuses on forests in Poland. After analyzing 17,820 forest sections where the soils were described as fluvisols, it was found that forest areas associated with fluvisols (typical, fertile soils of river valleys) are quite well recognized and protected in Poland. Most (55%) forested fluvisols are located in Natura 2000 sites (an important European network of biodiversity hotspots), 4% in nature reserves, and 1% in national parks. Additionally, the main forest habitat type associated with fluvisols is riparian forest, composed mainly of Quercus, Ulmus, and Fraxinus, which is protected as Natura 2000 habitat type 91F0. Preserving the sustainability of the forest is also a form of soil protection. Despite the identification of soils and forests in river valleys, as well as appropriate legal tools, their protection may be ineffective due to the fragmentation of forms of protection and the lack of a coherent system for managing river valleys. Because the conservation status of the river valleys is also influenced by the management of areas located outside the river valleys, in order to protect river valley ecosystems, integrated conservation plans for entire catchments should be implemented. Due to potential conflicts related to the management of areas with diverse expectations of local communities, it would be advisable for such plans to be created by local experts but under the supervision of a specialist/specialists from outside the area covered by a given river basin. Full article
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12 pages, 978 KiB  
Article
The Impact of Caregiver Pressure to Eat on Food Neophobia in Children with Autism Spectrum Disorder: A Cross-Sectional Study
by Qingqing Xie, Cuiting Yong, Caihong Xiang, Yue Xi, Jiaqi Huo, Jiajing Liang, Hanshuang Zou, Yunfeng Pan, Minchan Wu and Qian Lin
Children 2024, 11(5), 528; https://doi.org/10.3390/children11050528 (registering DOI) - 28 Apr 2024
Abstract
(1) Background: With autistic children’s high pervasiveness of eating problems and inappropriate feeding behaviors by their caregivers, this study wanted to inspect the connection between caregivers’ pressure to eat and food neophobia in these children. (2) Methods: Cross-sectional overview of 160 guardians of [...] Read more.
(1) Background: With autistic children’s high pervasiveness of eating problems and inappropriate feeding behaviors by their caregivers, this study wanted to inspect the connection between caregivers’ pressure to eat and food neophobia in these children. (2) Methods: Cross-sectional overview of 160 guardians of kids aged 2 to 7 years. After one-on-one questioning by the researcher, the collected information on the socio-demographic characteristics of the children with autism, caregiver feeding behavior, and new food neophobia (FN) scores was entered into the Questionnaire Star system. (3) Results: The mean FN score was 25.56 ± 6.46. The caregiver’s pressure to eat positively related to children’s FN (β = 0.164 95% CI, 0.078, 2.163). In these children, we found a negative correlation between FN score and the frequency of vegetable intake (p ≤ 0.001), fruit intake (p ≤ 0.05), aquatic product intake (p ≤ 0.05), and dietary diversity score (p ≤ 0.01), and positively correlated with the frequency of snack intake (p ≤ 0.05). (4) Conclusions: Caregiver pressure to eat was positively associated with high levels of FN in Chinese kids with ASD, which in turn negatively impacted dietary quality. To improve eating habits, caregivers should reconsider their feeding strategies and avoid using forceful methods to ease food neophobia in these children. Full article
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16 pages, 5483 KiB  
Article
Extracellular Nicotinamide Phosphoribosyltransferase Is a Therapeutic Target in Experimental Necrotizing Enterocolitis
by Melissa D. Halpern, Akash Gupta, Nahla Zaghloul, Senthilkumar Thulasingam, Christine M. Calton, Sara M. Camp, Joe G. N. Garcia and Mohamed Ahmed
Biomedicines 2024, 12(5), 970; https://doi.org/10.3390/biomedicines12050970 (registering DOI) - 28 Apr 2024
Abstract
Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency of prematurity. Postulated mechanisms leading to inflammatory necrosis of the ileum and colon include activation of the pathogen recognition receptor Toll-like receptor 4 (TLR4) and decreased levels of transforming growth factor beta (TGFβ). Extracellular [...] Read more.
Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency of prematurity. Postulated mechanisms leading to inflammatory necrosis of the ileum and colon include activation of the pathogen recognition receptor Toll-like receptor 4 (TLR4) and decreased levels of transforming growth factor beta (TGFβ). Extracellular nicotinamide phosphoribosyltransferase (eNAMPT), a novel damage-associated molecular pattern (DAMP), is a TLR4 ligand and plays a role in a number of inflammatory disease processes. To test the hypothesis that eNAMPT is involved in NEC, an eNAMPT-neutralizing monoclonal antibody, ALT-100, was used in a well-established animal model of NEC. Preterm Sprague–Dawley pups delivered prematurely from timed-pregnant dams were exposed to hypoxia/hypothermia and randomized to control—foster mother dam-fed rats, injected IP with saline (vehicle) 48 h after delivery; control + mAB—foster dam-fed rats, injected IP with 10 µg of ALT-100 at 48 h post-delivery; NEC—orally gavaged, formula-fed rats injected with saline; and NEC + mAb—formula-fed rats, injected IP with 10 µg of ALT-100 at 48 h. The distal ileum was processed 96 h after C-section delivery for histological, biochemical, molecular, and RNA sequencing studies. Saline-treated NEC pups exhibited markedly increased fecal blood and histologic ileal damage compared to controls (q < 0.0001), and findings significantly reduced in ALT-100 mAb-treated NEC pups (q < 0.01). Real-time PCR in ileal tissues revealed increased NAMPT in NEC pups compared to pups that received the ALT-100 mAb (p < 0.01). Elevated serum levels of tumor necrosis factor alpha (TNFα), interleukin 6 (IL-6), interleukin-8 (IL-8), and NAMPT were observed in NEC pups compared to NEC + mAb pups (p < 0.01). Finally, RNA-Seq confirmed dysregulated TGFβ and TLR4 signaling pathways in NEC pups that were attenuated by ALT-100 mAb treatment. These data strongly support the involvement of eNAMPT in NEC pathobiology and eNAMPT neutralization as a strategy to address the unmet need for NEC therapeutics. Full article
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17 pages, 2973 KiB  
Article
Predicting the Conversion from Mild Cognitive Impairment to Alzheimer’s Disease Using an Explainable AI Approach
by Gerasimos Grammenos, Aristidis G. Vrahatis, Panagiotis Vlamos, Dean Palejev, Themis Exarchos and for the Alzheimer’s Disease Neuroimaging Initiative
Information 2024, 15(5), 249; https://doi.org/10.3390/info15050249 (registering DOI) - 28 Apr 2024
Abstract
Mild Cognitive Impairment (MCI) is a cognitive state frequently observed in older adults, characterized by significant alterations in memory, thinking, and reasoning abilities that extend beyond typical cognitive decline. It is worth noting that around 10–15% of individuals with MCI are projected to [...] Read more.
Mild Cognitive Impairment (MCI) is a cognitive state frequently observed in older adults, characterized by significant alterations in memory, thinking, and reasoning abilities that extend beyond typical cognitive decline. It is worth noting that around 10–15% of individuals with MCI are projected to develop Alzheimer’s disease, effectively positioning MCI as an early stage of Alzheimer’s. In this study, a novel approach is presented involving the utilization of eXtreme Gradient Boosting to predict the onset of Alzheimer’s disease during the MCI stage. The methodology entails utilizing data from the Alzheimer’s Disease Neuroimaging Initiative (ADNI). Through the analysis of longitudinal data, spanning from the baseline visit to the 12-month follow-up, a predictive model was constructed. The proposed model calculates, over a 36-month period, the likelihood of progression from MCI to Alzheimer’s disease, achieving an accuracy rate of 85%. To further enhance the precision of the model, this study implements feature selection using the Recursive Feature Elimination technique. Additionally, the Shapley method is employed to provide insights into the model’s decision-making process, thereby augmenting the transparency and interpretability of the predictions. Full article
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19 pages, 4716 KiB  
Article
TMT-Based Quantitative Proteomics Reveal the Metabolic Changes Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × G. eckloni
by Yanzhen Dong, Junming Zhou, Dayong Xu, Yun Zhao and Dongming Qi
Fishes 2024, 9(5), 158; https://doi.org/10.3390/fishes9050158 (registering DOI) - 28 Apr 2024
Abstract
Hybrid breeding is an effective approach to generate better varieties and prevent variety degradation. The present study investigated the metabolic changes underlying growth superiority in the novel Gymnocypris hybrid (GH), Gymnocypris przewalskii ♀ (GP) × G. eckloni ♂ (GE). The ranking of survival [...] Read more.
Hybrid breeding is an effective approach to generate better varieties and prevent variety degradation. The present study investigated the metabolic changes underlying growth superiority in the novel Gymnocypris hybrid (GH), Gymnocypris przewalskii ♀ (GP) × G. eckloni ♂ (GE). The ranking of survival rate was GH > GE > GP, whereas the ranking of growth rate was GE > GH > GP. A proteomic analysis of G. hybrid and its parents was conducted to elucidate the metabolic changes underlying growth superiority. Identified pathways were primarily associated with amino acid, carbohydrate, energy, lipid, and nucleotide metabolism. These metabolic pathways, which are closely associated with growth, are controlled through regulation of the expression of numerous proteins, including adenosylhomocysteinase, hydroxypyruvate reductase, glutamate-cysteine ligase, L-lactate dehydrogenase, creatine kinase, GDP-L-fucose synthase, pyruvate kinase, fructose-bisphosphate aldolase, carbonic anhydrase, phosphopyruvate hydratase, phosphoglycerate kinase, S-(hydroxymethyl) glutathione dehydrogenase, and AMP deaminase. Real-time PCR assays showed that the level of mRNA expression of differentially expressed genes was positively correlated with growth. Proteins that were differentially expressed in GH exhibited fewer differences from GP and more differences from GE. These data are the first to reveal the molecular mechanism whereby growth is regulated in G. hybrid and its parents at the protein level. The study thus provides important information for genetic breeding and improvement of G. hybrid for aquaculture production. Full article
(This article belongs to the Section Genetics and Biotechnology)
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15 pages, 3605 KiB  
Article
Inversion Tillage Combined with Organic Fertilizer Application Increased Maize Yield via Improving Soil Pore Structure and Enzymatic Activity in Haplic Chernozem
by Chunzhu Liu, Xiaozeng Han, Xu Chen, Jun Yan, Xinchun Lu, Bo Song, Wei Wang, Wenxiu Zou and Xianfa Ma
Agronomy 2024, 14(5), 927; https://doi.org/10.3390/agronomy14050927 (registering DOI) - 28 Apr 2024
Abstract
Inversion tillage and organic fertilizer application can break the plow pan and improve soil quality. However, the effects of combining these practices on the soil microbial resource limitation and maize yield in Haplic Chernozem are unclear. In this research, a field experiment was [...] Read more.
Inversion tillage and organic fertilizer application can break the plow pan and improve soil quality. However, the effects of combining these practices on the soil microbial resource limitation and maize yield in Haplic Chernozem are unclear. In this research, a field experiment was established in 2018, and soil samples were collected in 2021 in Longjiang County in Northeast China, which is a Haplic Chernozem region. Four treatments comprising conventional tillage (T15), conventional tillage with organic fertilizer (T15+M), inversion tillage (T35), and inversion tillage with organic fertilizer (T35+M) were randomly arranged with four replications. Compared with T15 and T15+M treatments, soil bulk density significantly decreased by 11.1–16.3% in the 15–35 cm layer under T35 and T35+M treatments, accompanied by the improvement in soil pore structure (e.g., soil porosity, circularity, and Euler number). T15+M treatment significantly increased soil organic carbon and soil nutrient contents by 11.1–16.3% and 3.9–24.5% in the 0–15 cm layer compared with other treatments. However, soil organic carbon, total nitrogen, available phosphorus content, microbial biomass, and enzymatic activities reached the maximum values in the 0–35 cm layer under T35+M treatment. In addition, T35+M treatment had the highest maize yield and sustainable yield index. Extracellular enzymatic stoichiometry suggested that soil microorganisms are generally co-limited by carbon and phosphorus in Haplic Chernozem. However, T35+M treatment significantly reduced soil microbial resource limitation, which was one important factor impacting maize yield and sustainability. Random-forest and partial least-squares path modeling showed that T35+M treatment could reduce soil microbial resource limitation and increase the stability and sustainability of the maize yield by improving soil available nutrients, microbial biomass, and pore structure. Therefore, the incorporation of inversion tillage and organic fertilizer is a suitable soil management practice in view of increasing soil quality and crop yields in a Haplic Chernozem region. Full article
(This article belongs to the Special Issue Effects of Arable Farming Measures on Soil Quality)
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17 pages, 7486 KiB  
Article
Protection Coordination Strategy for the Distributed Electric Aircraft Propulsion Systems
by Anil Kumar Reddy Siddavatam, Kaushik Rajashekara, Hao Huang and Fred Wang
World Electr. Veh. J. 2024, 15(5), 187; https://doi.org/10.3390/wevj15050187 (registering DOI) - 28 Apr 2024
Abstract
The current trend in distributed electric aircraft propulsion systems is to utilize the DC bus system at higher voltage levels than conventional aircraft systems. With Boeing and Airbus utilizing the +/−270 V bipolar DC bus system, the research on high-voltage systems is increasing [...] Read more.
The current trend in distributed electric aircraft propulsion systems is to utilize the DC bus system at higher voltage levels than conventional aircraft systems. With Boeing and Airbus utilizing the +/−270 V bipolar DC bus system, the research on high-voltage systems is increasing gradually, with voltage levels ranging from 1 to 10 kV systems or +/−0.5 to +/−5 kV DC bus systems. These voltage levels present considerable challenges to the distributed electric aircraft propulsion systems. In addition to partial discharge effects, there are other challenges, particularly the challenge associated with effectively limiting short-circuit fault currents due to the low cable impedance of the distribution system. The cable impedance is a significant factor that determines the fault current during fault conditions. Due to the low impedance, there is a sharp increase in fault current, necessitating an enhanced protection strategy, which ensures that the system is adequately protected. This paper introduces a coordinated protection strategy specifically designed for distributed electric aircraft propulsion systems to mitigate or prevent short-circuit faults. The proposed algorithm utilizes an I2t-based strategy and the current-limiting-based strategy to protect the system from short-circuit faults and overload conditions. Redundant backup protection is also included in the algorithm in case the circuit breaker fails to operate. Full article
(This article belongs to the Special Issue Electric and Hybrid Electric Aircraft Propulsion Systems)
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23 pages, 1966 KiB  
Article
Imagine and Imitate: Cost-Effective Bidding under Partially Observable Price Landscapes
by Xiaotong Luo, Yongjian Chen, Shengda Zhuo, Jie Lu, Ziyang Chen, Lichun Li, Jingyan Tian, Xiaotong Ye and Yin Tang
Big Data Cogn. Comput. 2024, 8(5), 46; https://doi.org/10.3390/bdcc8050046 (registering DOI) - 28 Apr 2024
Abstract
Real-time bidding has become a major means for online advertisement exchange. The goal of a real-time bidding strategy is to maximize the benefits for stakeholders, e.g., click-through rates or conversion rates. However, in practise, the optimal bidding strategy for real-time bidding is constrained [...] Read more.
Real-time bidding has become a major means for online advertisement exchange. The goal of a real-time bidding strategy is to maximize the benefits for stakeholders, e.g., click-through rates or conversion rates. However, in practise, the optimal bidding strategy for real-time bidding is constrained by at least three aspects: cost-effectiveness, the dynamic nature of market prices, and the issue of missing bidding values. To address these challenges, we propose Imagine and Imitate Bidding (IIBidder), which includes Strategy Imitation and Imagination modules, to generate cost-effective bidding strategies under partially observable price landscapes. Experimental results on the iPinYou and YOYI datasets demonstrate that IIBidder reduces investment costs, optimizes bidding strategies, and improves future market price predictions. Full article
(This article belongs to the Special Issue Business Intelligence and Big Data in E-commerce)
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14 pages, 9810 KiB  
Article
Unveiling the Genomic Landscape of Intraductal Carcinoma of the Prostate Using Spatial Gene Expression Analysis
by Ryuta Watanabe, Noriyoshi Miura, Mie Kurata, Riko Kitazawa, Tadahiko Kikugawa and Takashi Saika
Int. J. Mol. Sci. 2024, 25(9), 4818; https://doi.org/10.3390/ijms25094818 (registering DOI) - 28 Apr 2024
Abstract
Intraductal carcinoma of the prostate (IDCP) has recently attracted increasing interest owing to its unfavorable prognoses. To effectively identify the IDCP-specific gene expression profile, we took a novel approach of characterizing a typical IDCP case using spatial gene expression analysis. A formalin-fixed, paraffin-embedded [...] Read more.
Intraductal carcinoma of the prostate (IDCP) has recently attracted increasing interest owing to its unfavorable prognoses. To effectively identify the IDCP-specific gene expression profile, we took a novel approach of characterizing a typical IDCP case using spatial gene expression analysis. A formalin-fixed, paraffin-embedded sample was subjected to Visium CytAssist Spatial Gene Expression analysis. IDCP within invasive prostate cancer sites was recognized as a distinct cluster separate from other invasive cancer clusters. Highly expressed genes defining the IDCP cluster, such as MUC6, MYO16, NPY, and KLK12, reflected the aggressive nature of high-grade prostate cancer. IDCP sites also showed increased hypoxia markers HIF1A, BNIP3L, PDK1, and POGLUT1; decreased fibroblast markers COL1A2, DCN, and LUM; and decreased immune cell markers CCR5 and FCGR3A. Overall, these findings indicate that the hypoxic tumor microenvironment and reduced recruitment of fibroblasts and immune cells, which reflect morphological features of IDCP, may influence the aggressiveness of high-grade prostate cancer. Full article
(This article belongs to the Special Issue Molecular Mechanisms Underlying the Progression of Prostate Cancer)
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18 pages, 348 KiB  
Article
Food Insecurity Is Associated with Diet Quality in Pregnancy: A Cross-Sectional Study
by Bree Whiteoak, Samantha L. Dawson, Leonie Callaway, Susan de Jersey, Victoria Eley, Joanna Evans, Alka Kothari, Severine Navarro and Danielle Gallegos
Nutrients 2024, 16(9), 1319; https://doi.org/10.3390/nu16091319 (registering DOI) - 28 Apr 2024
Abstract
Household food insecurity (HFI) and poorer prenatal diet quality are both associated with adverse perinatal outcomes. However, research assessing the relationship between HFI and diet quality in pregnancy is limited. A cross-sectional online survey was conducted to examine the relationship between HFI and [...] Read more.
Household food insecurity (HFI) and poorer prenatal diet quality are both associated with adverse perinatal outcomes. However, research assessing the relationship between HFI and diet quality in pregnancy is limited. A cross-sectional online survey was conducted to examine the relationship between HFI and diet quality among 1540 pregnant women in Australia. Multiple linear regression models were used to examine the associations between HFI severity (marginal, low, and very low food security compared to high food security) and diet quality and variety, adjusting for age, education, equivalised household income, and relationship status. Logistic regression models were used to assess the associations between HFI and the odds of meeting fruit and vegetable recommendations, adjusting for education. Marginal, low, and very low food security were associated with poorer prenatal diet quality (adj β = −1.9, −3.6, and −5.3, respectively; p < 0.05), and very low food security was associated with a lower dietary variety (adj β = −0.5, p < 0.001). An association was also observed between HFI and lower odds of meeting fruit (adjusted odds ratio [AOR]: 0.61, 95% CI: 0.49–0.76, p < 0.001) and vegetable (AOR: 0.40, 95% CI: 0.19–0.84, p = 0.016) recommendations. Future research should seek to understand what policy and service system changes are required to reduce diet-related disparities in pregnancy. Full article
(This article belongs to the Section Nutrition and Public Health)

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