The 2023 MDPI Annual Report has
been released!
 
18 pages, 790 KiB  
Article
Multiplex Detection of Seven Staphylococcal Enterotoxins Using Liquid Chromatography–Mass Spectrometry Combined with a Novel Capture Molecule
by Jing Lv, Tingting Liu, Xinyu Fang, Songyang Han, Lina Dong, Jiaxin Li, Jing Wang, Jinglin Wang, Shan Gao, Lin Kang and Wenwen Xin
Separations 2024, 11(5), 136; https://doi.org/10.3390/separations11050136 - 29 Apr 2024
Abstract
Food poisoning caused by Staphylococcal enterotoxins (SEs) is prevalent globally, making efficient detection of these toxins very important. Traditionally, liquid chromatography–mass spectrometry required immunosorbent enrichment by magnetic bead-coupled antibodies obtained by animal-specific immunization. However, this method is time-consuming and costly. In this study, [...] Read more.
Food poisoning caused by Staphylococcal enterotoxins (SEs) is prevalent globally, making efficient detection of these toxins very important. Traditionally, liquid chromatography–mass spectrometry required immunosorbent enrichment by magnetic bead-coupled antibodies obtained by animal-specific immunization. However, this method is time-consuming and costly. In this study, two recombinant protein capture molecules were designed based on the principle of toxins binding to Major Histocompatibility Complex (MHCII) and T cell receptor (TCR) molecules. The two capture molecules are called MHCII and MHCII-D10. The design of the MHCII and TCR-D10 was achieved through searching for the binding site protein sequence of Staphylococcal enterotoxins in the relevant literature, and MHCII-D10 was to link MHCII sequence with TCR-D10 sequence using linker (G4S)3 linking peptide. These capture molecules were shown to effectively bind to seven types of toxins and to capture SEs in various matrices. The digestion time, ratio, and temperature were further optimized, reducing the overall digestion time to just 2 h. The specificity, linearity, sensitivity, precision (RSD%), and recovery of the two methods were verified by liquid chromatography–mass spectrometry. When the MHCII and MHCII-D10 captured the toxins, the limit of quantification (LOD) in the 1 × PBS, plasma, and milk matrices ranged from 1.5625 to 100 fmol/µL, with the recovery rate ranging from 18.4% to 96%. The design of these capture molecules eliminates the need for animal-specific immunization, simplifying the pre-detection process and avoiding ethical concerns. This development holds significant promise for clinical diagnosis and reference. Full article
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13 pages, 714 KiB  
Article
Energy Budget Characterisation of the Optimal Disturbance in Stratified Shear Flow
by Larry E. Godwin, Philip M. J. Trevelyan, Takeshi Akinaga and Sotos C. Generalis
Fluids 2024, 9(5), 106; https://doi.org/10.3390/fluids9050106 - 29 Apr 2024
Abstract
Stratified Taylor–Couette flow (STCF) undergoes transient growth. Recent studies have shown that there exists transient amplification in the linear regime of counter-rotating STCF. The kinetic budget of the optimal transient perturbation is analysed numerically to simulate the interaction of the shear production (SP), [...] Read more.
Stratified Taylor–Couette flow (STCF) undergoes transient growth. Recent studies have shown that there exists transient amplification in the linear regime of counter-rotating STCF. The kinetic budget of the optimal transient perturbation is analysed numerically to simulate the interaction of the shear production (SP), buoyancy flux (BP), and other energy components that contributes to the total optimal transient kinetic energy. These contributions affect the total energy by influencing the perturbation to extract kinetic energy (KE) from the mean flow. The decay of the amplification factor resulted from the positive amplification of both BP and SP, while the growth is attributed to the negative and positive amplification of BP and SP, respectively. The optimal SP is positively amplified, implying that there is the possibility of constant linear growth. These findings agree with the linear growth rate for increasing values of Grashof number. Full article
(This article belongs to the Collection Challenges and Advances in Heat and Mass Transfer)
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16 pages, 969 KiB  
Article
Chromatographic and Thermal Characteristics, and Hydrolytic and Oxidative Stability of Commercial Pomegranate Seed Oil
by Marta Siol, Agnieszka Dudek, Joanna Bryś, Diana Mańko-Jurkowska, Eliza Gruczyńska-Sękowska, Sina Makouie, Bharani Kumar Palani, Marko Obranović and Piotr Koczoń
Foods 2024, 13(9), 1370; https://doi.org/10.3390/foods13091370 - 29 Apr 2024
Abstract
The current investigations were aimed at the determination of the hydrolytic and oxidative stability of commercial pomegranate seed oils provided by four different producers, and to assess the oils’ primary quality parameters. During storage, many changes occur in oils that can significantly affect [...] Read more.
The current investigations were aimed at the determination of the hydrolytic and oxidative stability of commercial pomegranate seed oils provided by four different producers, and to assess the oils’ primary quality parameters. During storage, many changes occur in oils that can significantly affect their quality. The oils were tested for acid and peroxide values, fatty acid profile, and their distribution between the sn-1,3 and sn-2 positions of triacylglycerols. The oxidative stability was also determined, and melting curves were plotted for the oils. The analyzed oils were stored for one month in a dark place at refrigerator temperature. Based on the obtained results, it was found that the acid values for most oils did not exceed the permissible level determined by the Codex Alimentarius. However, in all oils, the peroxide value exceeded the permissible level set by the standard EN ISO 3960:2017-03 and the Codex Alimentarius after the one-month storage period. The examined pomegranate seed oils were found to be valuable sources of polyunsaturated fatty acids, especially punicic acid, which was the most abundant fatty acid present in these oils. In all analyzed oils, linoleic acid predominated in the sn-2 position of the triacylglycerols. Pomegranate seed oils did not exhibit good oxidative stability, as the oxidation induction times for all tested oils were very short. The storage period significantly affected the content of the primary oxidation products and oxidative stability of the oils. Full article
(This article belongs to the Special Issue Food Lipids — Chemistry, Nutrition and Biotechnology)
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13 pages, 259 KiB  
Article
Nutritional Value and Physicochemical Properties of Male and Female Broad-Breasted Bronze Turkey Muscle
by Anna Czech, Piotr Domaradzki, Mateusz Niedzielak and Joanna Stadnik
Foods 2024, 13(9), 1369; https://doi.org/10.3390/foods13091369 - 29 Apr 2024
Abstract
Colored varieties of turkeys, such as the broad-breasted Bronze, not currently subject to intensive breeding work, are kept only in amateur breeding and treated rather as ornamental poultry. They are raised in extensive systems, which undoubtedly affects the quality of the meat obtained. [...] Read more.
Colored varieties of turkeys, such as the broad-breasted Bronze, not currently subject to intensive breeding work, are kept only in amateur breeding and treated rather as ornamental poultry. They are raised in extensive systems, which undoubtedly affects the quality of the meat obtained. Consumers are looking for meat with specific and unique sensory qualities; hence, the interest in meat from turkeys with a slower-than-typical growth rate, such as the broad-breasted Bronze, is justified. The object of this research was to analyze the physicochemical properties and nutritional value (amino acid, fatty acid, and antioxidant profile) of the breast and thigh muscles of broad-breasted Bronze turkeys with regard to gender. It was shown that gender had little effect on muscle chemical composition, amino acid, and fatty acid content (p > 0.05), as well as most oxido-reduction indices. However, significant differences were noted in muscle quality traits such as color brightness (L*; turkeys > indors; p = 0.023), proportion of red (a*; turkeys < indors; p = 0.048) and yellow (b*; turkeys > indors; p = 0.039), and water absorption (turkeys < indors; p = 0.009). The type of muscle also had a significant effect on quality characteristics. Higher a*, b*, C*, pH, water absorption, and thermal leakage were shown in the femoral muscle (p < 0.001), while L* and h were higher in the pectoral muscle (p < 0.001). Turkey meat was characterized by a high proportion of unsaturated fatty acids (MUFA + PUFA ~68%), favorable FA index values, and low lipid oxidation indices. Thigh muscles (especially turkey) were more caloric, and contained significantly (p < 0.001) more fat and all major FA groups. Breast muscles (especially of turkeys) were characterized by a high protein content (about 25%) and a high proportion of essential amino acids. The data obtained indicate that broad-breasted Bronze turkeys can provide high-quality meat, and are an excellent option for meeting modern consumer needs. Full article
(This article belongs to the Section Meat)
5 pages, 201 KiB  
Editorial
Metal Complexes with N-donor Ligands
by László Kótai
Inorganics 2024, 12(5), 130; https://doi.org/10.3390/inorganics12050130 - 29 Apr 2024
Abstract
Complexes of transition and non-transition metals with a wide variety of N-donor ligands (like ammonia, amines, urea derivatives, Schiff bases, or N-heterocycles) comprise a highly important class of compounds in chemistry, biochemistry, material science, and the chemical industry [...] Full article
(This article belongs to the Special Issue Metal Complexes with N-donor Ligands)
17 pages, 1358 KiB  
Article
Adaptive Parallel Scheduling Scheme for Smart Contract
by Wenjin Yang, Meng Ao, Jing Sun, Guoan Wang, Yongxuan Li, Chunhai Li and Zhuguang Shao
Mathematics 2024, 12(9), 1347; https://doi.org/10.3390/math12091347 - 29 Apr 2024
Abstract
With the increasing demand for decentralized systems and the widespread usage of blockchain, low throughput and high latency have become the biggest stumbling blocks in the development of blockchain systems. This problem seriously hinders the expansion of blockchain and its application in production. [...] Read more.
With the increasing demand for decentralized systems and the widespread usage of blockchain, low throughput and high latency have become the biggest stumbling blocks in the development of blockchain systems. This problem seriously hinders the expansion of blockchain and its application in production. Most existing smart contract scheduling solutions use static feature analysis to prevent contract conflicts during parallel execution. However, the conflicts between transactions are complex; static feature analysis is not accurate enough. In this paper, we first build the dependency between smart contracts by analyzing the features. After numerous experiments, we propose a conflict model to adjust the relationship between threads and conflict to achieve high throughput and low latency. Based on these works, we propose adaptive parallel scheduling for smart contracts on the blockchain. Our adaptive parallel scheduling can distinguish conflicts between smart contracts and dynamically adjust the execution strategy of smart contracts based on the conflict factors we define. We implement our scheme on ChainMaker, one of the most popular open-source permissioned blockchains, and build experiments to verify our solution. Regarding latency, our solution demonstrates remarkable efficiency compared with the fully parallel scheme, particularly in high-conflict transaction scenarios, where our solution achieves latency levels just one-twentieth of the fully parallel scheme. Regarding throughput, our solution significantly outperforms the fully parallel scheme, achieving 30 times higher throughput in high-conflict transaction scenarios. These results highlight the superior performance and effectiveness of our solution in addressing latency and throughput challenges, particularly in environments with high transaction conflicts. Full article
(This article belongs to the Special Issue New Advances in Coding Theory and Cryptography, 2nd Edition)
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26 pages, 8121 KiB  
Article
Exploring Dynamic Spalling Behavior in Rock–Shotcrete Combinations: A Theoretical and Numerical Investigation
by Lin Luo, Yichao Rui, Jiadong Qiu, Chongjin Li, Xiong Liu and Cong Chen
Mathematics 2024, 12(9), 1346; https://doi.org/10.3390/math12091346 - 29 Apr 2024
Abstract
Spalling is a widespread dynamic disaster during blasting excavation in underground engineering. To clarify the coupled dynamic response and spalling behavior of an underground tunnel with a spray anchor, an investigation based on the rock–shotcrete combination was conducted using theoretical and numerical methods. [...] Read more.
Spalling is a widespread dynamic disaster during blasting excavation in underground engineering. To clarify the coupled dynamic response and spalling behavior of an underground tunnel with a spray anchor, an investigation based on the rock–shotcrete combination was conducted using theoretical and numerical methods. The mathematical representation of stress wave propagation between rock and shotcrete was deduced based on the elastic stress wave theory. A novel method for predicting the location and time of initial spalling in a rock–shotcrete combination was proposed. A numerical simulation was conducted to verify the validity of the proposed theoretical method. In addition, the effect of the material’s tensile strength, the loading amplitude, and the thickness of shotcrete on the stress evolution and spalling characteristics was studied. The results demonstrate that the initial spalling locations are sensitive to the relationship between the normalized tensile strength of the rock, shotcrete, and interface. A high incident amplitude can cause the initial spalling in rock, and the shotcrete or rock–shotcrete interface can cause initial spalling due to a low incident amplitude. The stress evolution and spalling characteristics are sensitive to the thickness of shotcrete. The location of the initial spalling failure changes with the thickness of the shotcrete. An appropriate increment in thickness and normalized strength of the shotcrete is beneficial to the dynamic stability of underground engineering. Full article
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18 pages, 8181 KiB  
Article
Abnormal Monitoring Data Detection Based on Matrix Manipulation and the Cuckoo Search Algorithm
by Zhenzhu Meng, Yiren Wang, Sen Zheng, Xiao Wang, Dan Liu, Jinxin Zhang and Yiting Shao
Mathematics 2024, 12(9), 1345; https://doi.org/10.3390/math12091345 - 29 Apr 2024
Abstract
Structural health monitoring is an effective method to evaluate the safety status of dams. Measurement error is an important factor which affects the accuracy of monitoring data modeling. Processing the abnormal monitoring data before data analysis is a necessary step to ensure the [...] Read more.
Structural health monitoring is an effective method to evaluate the safety status of dams. Measurement error is an important factor which affects the accuracy of monitoring data modeling. Processing the abnormal monitoring data before data analysis is a necessary step to ensure the reliability of the analysis. In this paper, we proposed a method to process the abnormal dam displacement monitoring data on the basis of matrix manipulation and Cuckoo Search algorithm. We first generate a scatter plot of the monitoring data and exported the matrix of the image. The scatter plot of monitoring data includes isolate outliers, clusters of outliers, and clusters of normal points. The gray scales of isolated outliers are reduced using Gaussian blur. Then, the isolated outliers are eliminated using Ostu binarization. We then use the Cuckoo Search algorithm to distinguish the clusters of outliers and clusters of normal points to identify the process line. To evaluate the performance of the proposed data processing method, we also fitted the data processed by the proposed method and by the commonly used 3-σ method using a regression model, respectively. Results indicate that the proposed method has a better performance in abnormal detection compared with the 3-σ method. Full article
(This article belongs to the Special Issue Anomaly and Novelty Detection and Explainability)
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23 pages, 7440 KiB  
Article
Design and Experimental Results Obtained with an Astragalus Digger Prototype
by Jianpu Du, Wei Sun, Ming Zhao, Juanling Wang and Petru Aurelian Simionescu
Agriculture 2024, 14(5), 701; https://doi.org/10.3390/agriculture14050701 (registering DOI) - 29 Apr 2024
Abstract
Traditional methods for harvesting medicinal materials with long roots, like Astragalus membranaceus, require extensive soil excavation, leading to problems like inefficient soil separation, low stemming rates, and blockages in conveyor chains. To address these challenges, this study introduces a prototype machine capable of [...] Read more.
Traditional methods for harvesting medicinal materials with long roots, like Astragalus membranaceus, require extensive soil excavation, leading to problems like inefficient soil separation, low stemming rates, and blockages in conveyor chains. To address these challenges, this study introduces a prototype machine capable of digging, separating soil, crushing soil, and collecting the medicinal materials in one continuous process. The paper focuses on the machine’s design and working principle, with theoretical analysis and calculations for key components like the digging shovel, multi-stage conveyor, and soil-crushing device. Specific structural parameters were determined, and the screening efficiency of the roller screen was analyzed using EDEM 2020 software, comparing scenarios with and without rollers. A motion model for the medicinal materials during conveyance was established, allowing for the determination of optimal linear velocity and mounting angle for the conveyor. Additionally, a motion model for the second-stage conveyor chain and rear soil-crushing device was used to optimize their placement, ensuring efficient soil crushing without affecting the thrown Astragalus. Compared to traditional Chinese medicine diggers, this machine boasts superior resistance reduction and soil-crushing capabilities. Compared with traditional harvesters, the drag-reducing and soil-crushing device of this machine is more efficient, reducing the damage to Astragalus during the harvesting process, reducing the labor intensity of farmers, and improving the quality and efficiency of Astragalus harvesting. Field experiments have shown that when the operating speed of the prototype is 1.0 m/s and the roller-screen speed is 130~150 rpm, the operating performance is optimal, and comparative experiments can be conducted under the optimal parameters. From the experimental results, it can be seen that the improved equipment has increased the bright-stem rate by about 4%, the digging and loosening rate by 97.42%, and the damage rate by 2.44%. The equipment design meets the overall design requirements, and all experimental indicators meet national and industry standards. This provides a reference for the optimization and improvement of the soil-crushing device and the structure of the Astragalus membranaceus harvester. Full article
(This article belongs to the Section Agricultural Technology)
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11 pages, 2913 KiB  
Communication
Overexpression of Larch SCL6 Inhibits Transitions from Vegetative Meristem to Inflorescence and Flower Meristem in Arabidopsis thaliana (L.) Heynh.
by Jun-Xia Xing, Qiao-Lu Zang, Zha-Long Ye, Li-Wang Qi, Ling Yang and Wan-Feng Li
Plants 2024, 13(9), 1232; https://doi.org/10.3390/plants13091232 - 29 Apr 2024
Abstract
SCARECROW-LIKE6 (SCL6) plays a role in the formation and maintenance of the meristem. In Larix kaempferi (Lamb.) Carr., an important afforestation tree species in China, SCL6 (LaSCL6) has two alternative splicing variants—LaSCL6-var1 and LaSCL6-var2—which are regulated by [...] Read more.
SCARECROW-LIKE6 (SCL6) plays a role in the formation and maintenance of the meristem. In Larix kaempferi (Lamb.) Carr., an important afforestation tree species in China, SCL6 (LaSCL6) has two alternative splicing variants—LaSCL6-var1 and LaSCL6-var2—which are regulated by microRNA171. However, their roles are still unclear. In this study, LaSCL6-var1 and LaSCL6-var2 were transformed into the Arabidopsis thaliana (L.) Heynh. genome, and the phenotypic characteristics of transgenic A. thaliana, including the germination percentage, root length, bolting time, flower and silique formation times, inflorescence axis length, and branch and silique numbers, were analyzed to reveal their functions. It was found that LaSCL6-var1 and LaSCL6-var2 overexpression shortened the root length by 41% and 31%, respectively, and increased the inflorescence axis length. Compared with the wild type, the bolting time in transgenic plants was delayed by approximately 2–3 days, the first flower and silique formation times were delayed by approximately 3–4 days, and the last flower and silique formation times were delayed by about 5 days. Overall, the life cycle in transgenic plants was prolonged by approximately 5 days. These results show that LaSCL6 overexpression inhibited the transitions from the vegetative meristem to inflorescence meristem and from the flower meristem to meristem arrest in A. thaliana, revealing the roles of LaSCL6-var1 and LaSCL6-var2 in the fate transition and maintenance of the meristem. Full article
(This article belongs to the Special Issue Molecular Biology and Bioinformatics of Forest Trees)
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38 pages, 1229 KiB  
Review
Sustainable Development versus Extractivist Deforestation in Tropical, Subtropical, and Boreal Forest Ecosystems: Repercussions and Controversies about the Mother Tree and the Mycorrhizal Network Hypothesis
by Tomas Gabriel Bas, Mario Luis Sáez and Nicolas Sáez
Plants 2024, 13(9), 1231; https://doi.org/10.3390/plants13091231 - 29 Apr 2024
Abstract
This research reviews the phenomenon of extractive deforestation as a possible trigger for cascade reactions that could affect part of the forest ecosystem and its biodiversity (surface, aerial, and underground) in tropical, subtropical, and boreal forests. The controversy and disparities in criteria generated [...] Read more.
This research reviews the phenomenon of extractive deforestation as a possible trigger for cascade reactions that could affect part of the forest ecosystem and its biodiversity (surface, aerial, and underground) in tropical, subtropical, and boreal forests. The controversy and disparities in criteria generated in the international scientific community around the hypothesis of a possible link between “mother trees” and mycorrhizal networks in coopetition for nutrients, nitrogen, and carbon are analyzed. The objective is to promote awareness to generate more scientific knowledge about the eventual impacts of forest extraction. Public policies are emphasized as crucial mediators for balanced sustainable development. Currently, the effects of extractive deforestation on forest ecosystems are poorly understood, which requires caution and forest protection. Continued research to increase our knowledge in molecular biology is advocated to understand the adaptation of biological organisms to the new conditions of the ecosystem both in the face of extractive deforestation and reforestation. The environmental impacts of extractive deforestation, such as the loss of biodiversity, soil degradation, altered water cycles, and the contribution of climate change, remain largely unknown. Long-term and high-quality research is essential to ensure forest sustainability and the preservation of biodiversity for future generations. Full article
(This article belongs to the Special Issue New Perspectives on New World Tropical Forests)
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12 pages, 1648 KiB  
Article
A Cluster of Peronospora parasitica 13-like (NBS-LRR) Genes Is Associated with Powdery Mildew (Erysiphe polygoni) Resistance in Mungbean (Vigna radiata)
by Pitsanupong Waengwan, Kularb Laosatit, Yun Lin, Tarika Yimram, Xingxing Yuan, Xin Chen and Prakit Somta
Plants 2024, 13(9), 1230; https://doi.org/10.3390/plants13091230 - 29 Apr 2024
Abstract
Powdery mildew (PM) caused by Erysiphe polygoni is an important foliar disease in mungbean (Vigna radiata). A previous study showed that QTL qPMRUM5-2 is a major locus for PM resistance in mungbean accession RUM5 (highly resistant). Bioinformatics analysis revealed that flanking [...] Read more.
Powdery mildew (PM) caused by Erysiphe polygoni is an important foliar disease in mungbean (Vigna radiata). A previous study showed that QTL qPMRUM5-2 is a major locus for PM resistance in mungbean accession RUM5 (highly resistant). Bioinformatics analysis revealed that flanking markers of the qPMRUM5-2 covered a region of 1.93 Mb. In this study, we conducted fine mapping for the qPMRUM5-2 using the F2 population of 1156 plants of the cross between Chai Nat 60 (CN60; highly susceptible) and RUM5. PM resistance evaluation was performed under field conditions using F2:3 lines grown in three different environments. QTL analyses consistently located the qPMRUM5-2 to a 0.09 cm interval on linkage group 6 between InDel markers VrLG6-InDel05 and VrLG6-InDel10, which corresponded to a 135.0 kb region on chromosome 8 containing nine predicted genes of which five were NBS-LRR-type genes Recognition of Peronospora parasitica 13-like protein (RPP13L). Whole-genome re-sequencing of RUM5 and CN60 showed polymorphisms in four RPP13L genes predictively cause substantial amino acid changes, rendering them important candidate genes for PM resistance. The InDel markers VrLG6-InDel05 and VrLG6-InDel10 flanking to the qPMRUM5-2 would be useful for marker-assisted breeding of PM resistance in the mungbean. Full article
(This article belongs to the Special Issue Advances in Legume Crops Research)
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13 pages, 1469 KiB  
Article
Homobrassinolide Delays Huanglongbing Progression in Newly Planted Citrus (Citrus sinensis) Trees
by Meritxell Pérez-Hedo, Alberto Urbaneja and Fernando Alférez
Plants 2024, 13(9), 1229; https://doi.org/10.3390/plants13091229 - 29 Apr 2024
Abstract
Huanglongbing (HLB), or citrus greening, is a devastating disease impacting citrus trees worldwide, with severe effects particularly noted in Florida. Current strategies to combat HLB focus on aggressive replanting, despite the high susceptibility of young trees to infection. In this context, it is [...] Read more.
Huanglongbing (HLB), or citrus greening, is a devastating disease impacting citrus trees worldwide, with severe effects particularly noted in Florida. Current strategies to combat HLB focus on aggressive replanting, despite the high susceptibility of young trees to infection. In this context, it is critical to explore agronomic practices that can enhance the health and resistance of young citrus trees to HLB. Here, we demonstrate that treatment with homobrassinolide (HBr), a type of brassinosteroid, in newly planted citrus (Citrus sinensis) trees can delay HLB infection and improve tree health amidst the high psyllid pressure conditions endemic to Florida. Our study reveals a significant reduction in HLB infection rates in HBr-treated trees compared to control trees, with only 25% of treated trees testing positive for HLB by six months, in contrast to 100% infection in untreated trees. This delay in infection may be attributed to HBr inducing an immune response and negatively impacting psyllid performance, as subsequently demonstrated in a greenhouse experiment. Our findings suggest that HBr applications could serve as a viable strategy to enhance the resilience of citrus production against HLB, underscoring the need for further investigation into their mechanisms of action and potential role in a comprehensive pest and disease management strategy. Full article
(This article belongs to the Special Issue Application of Agrochemical Technologies in Crop Protection)
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17 pages, 4845 KiB  
Article
Optimization of Shared Electric Scooter Deployment Stations Based on Distance Tolerance
by Jianwei Yue, Yingqiu Long, Shaohua Wang and Haojian Liang
ISPRS Int. J. Geo-Inf. 2024, 13(5), 147; https://doi.org/10.3390/ijgi13050147 - 29 Apr 2024
Abstract
The proliferation of shared electric scooters (E-scooters) has brought convenience to urban transportation but has also introduced challenges such as disorderly parking and an imbalance between supply and demand. Given the current inconsistent quantity and spatial distribution of shared E-scooters, coupled with inadequate [...] Read more.
The proliferation of shared electric scooters (E-scooters) has brought convenience to urban transportation but has also introduced challenges such as disorderly parking and an imbalance between supply and demand. Given the current inconsistent quantity and spatial distribution of shared E-scooters, coupled with inadequate research on deployment stations selection, we propose a novel maximal covering location problem (MCLP) based on distance tolerance. The model aims to maximize the coverage of user demand while minimizing the sum of distances from users to deployment stations. A deep reinforcement learning (DRL) was devised to address this optimization model. An experiment was conducted focusing on areas with high concentrations of shared E-scooter trips in Chicago. The solutions of location selection were obtained by DRL, the Gurobi solver, and the genetic algorithm (GA). The experimental results demonstrated the effectiveness of the proposed model in optimizing the layout of shared E-scooter deployment stations. This study provides valuable insights into facility location selection for urban shared transportation tools, and showcases the efficiency of DRL in addressing facility location problems (FLPs). Full article
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11 pages, 2529 KiB  
Article
Synthesis and Evaluation of 5-(Heteroarylmethylene)hydantoins as Glycogen Synthase Kinase-3β Inhibitors
by Nicholas O. Schneider, Kendra Gilreath, Daniel J. Burkett, Martin St. Maurice and William A. Donaldson
Pharmaceuticals 2024, 17(5), 570; https://doi.org/10.3390/ph17050570 (registering DOI) - 29 Apr 2024
Abstract
Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase which plays a center role in the phosphorylation of a wide variety of proteins, generally leading to their inactivation. As such, GSK-3 is viewed as a therapeutic target. An ever-increasing number of small organic molecule [...] Read more.
Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase which plays a center role in the phosphorylation of a wide variety of proteins, generally leading to their inactivation. As such, GSK-3 is viewed as a therapeutic target. An ever-increasing number of small organic molecule inhibitors of GSK-3 have been reported. Phenylmethylene hydantoins are known to exhibit a wide range of inhibitory activities including for GSK-3β. A family of fourteen 2-heterocycle substituted methylene hydantoins (14, 1729) were prepared and evaluated for the inhibition of GSK-3β at 25 μM. The IC50 values of five of these compounds was determined; the two best inhibitors are 5-[(4′-chloro-2-pyridinyl)methylene]hydantoin (IC50 = 2.14 ± 0.18 μM) and 5-[(6′-bromo-2-pyridinyl)methylene]hydantoin (IC50 = 3.39 ± 0.16 μM). The computational docking of the compounds with GSK-3β (pdb 1q41) revealed poses with hydrogen bonding to the backbone at Val135. The 5-[(heteroaryl)methylene]hydantoins did not strongly inhibit other metalloenzymes, demonstrating poor inhibitory activity against matrix metalloproteinase-12 at 25 μM and against human carbonic anhydrase at 200 μM, and were not inhibitors for Staphylococcus aureus pyruvate carboxylase at concentrations >1000 μM. Full article
(This article belongs to the Special Issue Nitrogen Containing Scaffolds in Medicinal Chemistry 2023)
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19 pages, 18704 KiB  
Article
Wind-Induced Aerodynamic Effects on Set-Back Tall Buildings Using LES
by Nan-Ting Yu, Wei-Hao Li, Yu Zheng and Wei-Bin Yuan
Buildings 2024, 14(5), 1252; https://doi.org/10.3390/buildings14051252 - 29 Apr 2024
Abstract
Set-back modification represents a dependable method to reducing the wind effects on high-rise buildings, but the flow mechanism and aerodynamic responses of set-back tall buildings have not been explored systematically. Using the square building as the benchmark model, six set-back building models with [...] Read more.
Set-back modification represents a dependable method to reducing the wind effects on high-rise buildings, but the flow mechanism and aerodynamic responses of set-back tall buildings have not been explored systematically. Using the square building as the benchmark model, six set-back building models with varying steps and sizes were chosen for numerical simulation in this study. The results of mean wind pressure and local wind force distribution, power spectral densities of base moment, flow field, and structural response of the proposed building models are compared and analysed using the Large Eddy Simulation (LES) technique. The results demonstrate that the aerodynamic performance of set-back tall buildings dramatically improved, with the actual optimization in the cross-wind direction being more significant. Generally, a single set-back measure can enhance the aerodynamic performance of high-rise buildings more effectively than a double set-back measure. The outcome of this study provides an aerodynamic design guide for set-back tall buildings. Full article
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16 pages, 1873 KiB  
Article
Assessing Brain Processing Deficits Using Neuropsychological and Vision-Specific Tests for Concussion
by Brent A. Harper and Rahul Soangra
Sports 2024, 12(5), 125; https://doi.org/10.3390/sports12050125 - 29 Apr 2024
Abstract
Introduction: Since verbal memory and visual processing transpire within analogous cerebral regions, this study assessed (i) if a visual function can predict verbal memory performance. It also hypothesized whether neurocognitive (e.g., ImPACT) tests focusing on the Visual Memory and Cognitive Efficacy Index will [...] Read more.
Introduction: Since verbal memory and visual processing transpire within analogous cerebral regions, this study assessed (i) if a visual function can predict verbal memory performance. It also hypothesized whether neurocognitive (e.g., ImPACT) tests focusing on the Visual Memory and Cognitive Efficacy Index will predict Verbal Memory scores and (ii) if vision metrics and age can identify individuals with a history of concussion. Finally, it also hypothesized that King–Devick and near point of convergence scores alongside age considerations will identify candidates with a prior reported history of concussion. Materials and methods: This observational cohort assessed 25 collegiate ice hockey players prior to the competitive season considering age (19.76 ± 1.42 years) and BMI (25.9 ± 3.0 kg/cm2). Hypothesis 1 was assessed using a hierarchical (sequential) multiple regression analysis, assessing the predictive capacity of Visual Memory and Cognitive Efficacy Index scores in relation to Verbal Memory scores. Hypothesis 2 utilized a binomial logistic regression to determine if King–Devick and near point of convergence scores predict those with a prior history of concussion. Results: Hypothesis 1 developed two models, where Model 1 included Visual Memory as the predictor, while Model 2 added the Cognitive Efficacy Index as a predictor for verbal memory scores. Model 1 significantly explained 41% of the variance. Results from Model 2 suggest that the Cognitive Efficacy Index explained an additional 24.4%. Thus, Model 2 was interpreted where only the Cognitive Efficacy Index was a significant predictor (p = 0.001). For every 1 unit increase in the Cognitive Efficacy Index, Verbal Memory increased by 41.16. Hypothesis 2’s model was significant, accounting for 37.9% of the variance in those with a history of concussion. However, there were no significant unique predictors within the model as age (Wald = 1.26, p = 0.261), King–Devick (Wald = 2.31, p = 0.128), and near point of convergence (Wald = 2.43, p = 0.119) were not significant predictors individually. Conclusions: The conflicting findings of this study indicate that baseline data for those with a history of concussion greater than one year may not be comparable to the same metrics during acute concussion episodes. Young athletes who sustain a concussion may be able to overcompensate via the visual system. Future prospective studies with larger sample sizes are required using the proposed model’s objective metrics. Full article
(This article belongs to the Special Issue Advances in Sports Injury Prevention and Rehabilitation Strategies)
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18 pages, 1348 KiB  
Review
Wearable Sensors and the Evaluation of Physiological Performance in Elite Field Hockey Players
by Francesca Latino and Francesco Tafuri
Sports 2024, 12(5), 124; https://doi.org/10.3390/sports12050124 - 29 Apr 2024
Abstract
Sports performance tracking has gained a lot of interest and widespread use in recent years, especially in elite and sub-elite sports. This makes it possible to improve the effectiveness of training, to calibrate and balance workloads according to real energy expenditure, and to [...] Read more.
Sports performance tracking has gained a lot of interest and widespread use in recent years, especially in elite and sub-elite sports. This makes it possible to improve the effectiveness of training, to calibrate and balance workloads according to real energy expenditure, and to reduce the likelihood of injuries due to excessive physical stress. In this context, the aim of this review was to map the scientific literature on wearable devices used in field hockey, evaluating their characteristics and the available evidence on their validity in measuring physiological and movement parameters. A systematic investigation was carried out by employing five electronic databases and search terms that incorporated field hockey, wearables, and performance analysis. Two independent reviewers conducted assessments of the 3401 titles and abstracts for inclusion, and at the end of the screening process, 102 full texts were analyzed. Lastly, a total of 23 research articles that specifically concentrated on field hockey were incorporated. The selected papers dealt with performance monitoring (6 papers), technical analysis and strategy game (6), injury prevention (1), and physiological measurements (10). To appraise the quality of the evaluations, the Oxford quality scoring system scale was employed. The extraction of information was carried out through the utilization of the participants, intervention, comparison, and outcomes (PICOS) format. The analysis encompassed research studies that implemented wearable devices during training and competitive events. Among elite field hockey competitions, GPS units were identified as the predominant wearable, followed by heart rate monitors. The intraclass correlation coefficient (ICC) related to wearable devices showed reasonably high between-trial ICCs ranging from 0.77 to 0.99. The utilization of wearable devices in field hockey primarily centers around the measurement of player activity profiles and physiological demands. The presence of discrepancies in sampling rates and performance bands makes it arduous to draw comparisons between studies. Nevertheless, this analysis attested to the fact that wearable devices are being employed for diverse applications in the realm of field hockey. Full article
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18 pages, 6800 KiB  
Article
Sensitivity of Runoff to Climatic Factors and the Attribution of Runoff Variation in the Upper Shule River, North-West China
by Ling Jia, Zuirong Niu, Rui Zhang and Yali Ma
Water 2024, 16(9), 1272; https://doi.org/10.3390/w16091272 - 29 Apr 2024
Abstract
Climate change and human activities exert significant impact on the mechanism of runoff generation and confluence. Comprehending the reasons of runoff change is crucial for the sustainable development of water resources. Taking the Upper Shule River as the research area, the M-K test [...] Read more.
Climate change and human activities exert significant impact on the mechanism of runoff generation and confluence. Comprehending the reasons of runoff change is crucial for the sustainable development of water resources. Taking the Upper Shule River as the research area, the M-K test and the moving t test were used to diagnose the runoff mutation time. Furthermore, the slope changing ratio of cumulative quantity method (SCRCQ), climate elasticity method, and Budyko equation were utilized to quantitatively evaluate the impacts and contribution rates of climate change and human activities. The following results were obtained: (1) The Upper Shule River experienced a significant increase in runoff from 1972 to 2021, with 1998 marking the year of abrupt change. (2) The runoff sensitivity showed a downward trend from 1972 to 2021. The main factor affecting the decrease in runoff sensitivity was the characteristic parameters of underlying surface (n), followed by precipitation (P), while the influence of potential evapotranspiration (ET0) was the weakest. (3) The response of runoff changes to runoff sensitivity and influencing factors were 90.32% and 9.68%, respectively. (4) The results of three attribution methods indicated that climate change was the primary factor causing the alteration of runoff in the Upper Shule River. The research results supplement the hydrological change mechanisms of the Upper Shule River and provide a scientific basis for future water resources management and flood control measures. Full article
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13 pages, 12788 KiB  
Article
Human-Altered Water and Carbon Cycles in the Lake Yangzong Basin since the Yuan Dynasty
by Huayong Li, Yuxue Jing, Hucai Zhang, Xuanxuan Shang, Lizeng Duan, Huayu Li, Donglin Li and Zhuohan Li
Water 2024, 16(9), 1271; https://doi.org/10.3390/w16091271 - 29 Apr 2024
Abstract
Due to the dual influence of climate change and human activities, the water cycle patterns in the lakesheds of the Yunnan karst plateau are undergoing significant changes, leading to increasingly prominent ecological issues. In the history of Lake Yangzong, an artificial water-diversion channel [...] Read more.
Due to the dual influence of climate change and human activities, the water cycle patterns in the lakesheds of the Yunnan karst plateau are undergoing significant changes, leading to increasingly prominent ecological issues. In the history of Lake Yangzong, an artificial water-diversion channel was excavated, altering the lake basin structure. Human activities have intensified, posing severe challenges to water resource supply and water security in recent decades. To investigate the significant increase in human activities, the temporal and phase changes, and the resulting transformation of the water and carbon cycles in the Lake Yangzong basin, we applied X-ray fluorescence spectroscopy (XRF) to scan elements continuously in a 10.2 m sediment core from this lake. By combining correlation analysis, principal component analysis (PCA), core chronology, and total organic carbon (TOC) content, we reconstructed the historical sequence of geochemical element contents in the Lake Yangzong catchment over the past 13,000 years. The results show that PC1 and PC2 contribute 78.4% and 10.3%, respectively, suggesting that erosion intensity is the main factor influencing the lake sedimentation process. From 13,400 to 680 cal a BP (calibrated years before the present), the sedimentation process in Lake Yangzong was mainly controlled by climatic conditions, with vegetation degradation during cold periods and relatively high erosion intensity in the watershed. During the Yuan dynasty, a province was established by the central government in Yunnan, promoting settlement and attracting a large number of immigrants from other provinces to Yunnan. Human activities in the Lake Yangzong basin began to intensify, surpassing natural changes and becoming the dominant force influencing the sedimentation process. In the Ming and Qing dynasties, the population and cultivated land area in Yunnan further increased, resulting in the significant exacerbation of erosion and soil loss in the watershed due to vegetation destruction. In the year 1388, the Tangchi Canal was excavated, transforming Lake Yangzong to an outflow lake, causing Ca2+ to be lost through the Tangchi Canal and preventing the formation of precipitation due to oversaturation. The research results indicate that human activities in the Lake Yangzong area have intensified since the Yuan dynasty, leading to increased erosion intensity. The excavation of the outflow canal transformed Lake Yangzong from an inland lake basin into an outflow state, simultaneously generating a significant transformation in the water and carbon cycling patterns in the watershed. Full article
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19 pages, 4368 KiB  
Article
Spatial and Temporal Distribution Characteristics and Potential Sources of Microplastic Pollution in China’s Freshwater Environments
by Hualong He, Sulin Cai, Siyuan Chen, Qiang Li, Pengwei Wan, Rumeng Ye, Xiaoyi Zeng, Bei Yao, Yanli Ji, Tingting Cao, Yunchao Luo, Han Jiang, Run Liu, Qi Chen, You Fang, Lu Pang, Yunru Chen, Weihua He, Yueting Pan, Gaozhong Pu, Jiaqin Zeng and Xingjun Tianadd Show full author list remove Hide full author list
Water 2024, 16(9), 1270; https://doi.org/10.3390/w16091270 - 29 Apr 2024
Abstract
Microplastic pollution is a research hotspot around the world. This study investigated the characteristics of microplastic pollution in the freshwater environments of 21 major cities across China. Through indoor and outdoor experimental analysis, we have identified the spatial and temporal distribution characteristics of [...] Read more.
Microplastic pollution is a research hotspot around the world. This study investigated the characteristics of microplastic pollution in the freshwater environments of 21 major cities across China. Through indoor and outdoor experimental analysis, we have identified the spatial and temporal distribution characteristics of microplastic pollution in China’s freshwater environments. Our findings indicate that the average concentration of microplastics in China’s freshwater environments is 3502.6 n/m3. The majority of these microplastics are fibrous (42.5%), predominantly smaller than 3 mm (28.1%), and mostly colored (64.7%). The primary chemical components of these microplastics are polyethylene (PE, 33.6%), polyvinyl chloride (PVC, 21.5%), polypropylene (PP, 16.8%), and polystyrene (PS, 15.6%). The abundance of microplastics in China’s freshwater environments generally tends to increase from west to east and from south to north, with the lowest concentration found in Xining, Qinghai (1737.5 n/m3), and the highest in Jiamusi, Heilongjiang (5650.0 n/m3). The distribution characteristics of microplastics are directly related to land use types, primarily concentrated in areas of intense human activity, including agricultural, transport, and urban land. Seasonal changes affect the abundance of microplastics, peaking in summer, followed by spring and autumn, mainly due to variations in rainfall, showing a positive correlation. Full article
(This article belongs to the Topic Microplastics Pollution)
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20 pages, 2613 KiB  
Article
Assessing Performance of Contemporary Plant-Based Diets against the UK Dietary Guidelines: Findings from the Feeding the Future (FEED) Study
by Izabella Lawson, Caroline Wood, Nandana Syam, Holly Rippin, Selina Dagless, Kremlin Wickramasinghe, Birdem Amoutzopoulos, Toni Steer, Timothy J. Key and Keren Papier
Nutrients 2024, 16(9), 1336; https://doi.org/10.3390/nu16091336 - 29 Apr 2024
Abstract
Uncertainty remains about the composition of contemporary plant-based diets and whether they provide recommended nutrient intakes. We established Feeding the Future (FEED), an up-to-date online cohort of UK adults following different plant-based diets and diets containing meat and fish. We recruited 6334 participants [...] Read more.
Uncertainty remains about the composition of contemporary plant-based diets and whether they provide recommended nutrient intakes. We established Feeding the Future (FEED), an up-to-date online cohort of UK adults following different plant-based diets and diets containing meat and fish. We recruited 6334 participants aged 18–99 [omnivores (1562), flexitarians (1349), pescatarians (568), vegetarians (1292), and vegans (1571)] between February 2022 and December 2023, and measured diet using a food frequency questionnaire and free text. We compared personal characteristics and dietary intakes between diet groups and assessed compliance with dietary guidelines. Most participants met UK dietary recommendations for fruit and vegetables, sodium, and protein, although protein intakes were lowest among vegetarians and vegans. Omnivores did not meet the fibre recommendation and only vegans met the saturated fat recommendation. All diet groups exceeded the free sugars recommendation. Higher proportions of vegetarians and vegans were below the estimated average requirements (EARs) for zinc, iodine, selenium, and, in vegans, vitamins A and B12, whereas calcium intakes were similar across the diet groups. People following plant-based diets showed good compliance with most dietary targets, and their risk for inadequate intakes of certain nutrients might be mitigated by improved dietary choices and/or food fortification. Full article
(This article belongs to the Section Nutrition Methodology & Assessment)
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15 pages, 3426 KiB  
Article
Targeting Non-Alcoholic Fatty Liver Disease with Hawthorn Ethanol Extract (HEE): A Comprehensive Examination of Hepatic Lipid Reduction and Gut Microbiota Modulation
by Tianyu Wang, Dawei Wang, Yinghui Ding, He Xu, Yue Sun, Jumin Hou and Yanrong Zhang
Nutrients 2024, 16(9), 1335; https://doi.org/10.3390/nu16091335 - 29 Apr 2024
Abstract
Recent studies have highlighted the lipid-lowering ability of hawthorn ethanol extract (HEE) and the role played by gut flora in the efficacy of HEE. Our study sought to explore the effects of HEE on non-alcoholic fatty liver disease (NAFLD) in normal flora and [...] Read more.
Recent studies have highlighted the lipid-lowering ability of hawthorn ethanol extract (HEE) and the role played by gut flora in the efficacy of HEE. Our study sought to explore the effects of HEE on non-alcoholic fatty liver disease (NAFLD) in normal flora and pseudo germ-free mice. The results showed that HEE effectively diminished hepatic lipid accumulation, ameliorated liver function, reduced inflammatory cytokine levels and blood lipid profiles, and regulated blood glucose levels. HEE facilitated triglyceride breakdown, suppressed fatty acid synthesis, and enhanced intestinal health by modulating the diversity of the gut microbiota and the production of short-chain fatty acids in the gut. In addition, HEE apparently helps to increase the presence of beneficial genera of bacteria, thereby influencing the composition of the gut microbiota, and the absence of gut flora affects the efficacy of HEE. These findings reveal the potential of hawthorn for the prevention and treatment of NAFLD and provide new perspectives on the study of functional plants to improve liver health. Full article
(This article belongs to the Special Issue Metabolic Features and Nutritional Interventions in Chronic Diseases)
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