The 2023 MDPI Annual Report has
been released!
 
14 pages, 963 KiB  
Review
Mitochondrial RNA Helicases: Key Players in the Regulation of Plant Organellar RNA Splicing and Gene Expression
by Ron Mizrahi and Oren Ostersetzer-Biran
Int. J. Mol. Sci. 2024, 25(10), 5502; https://doi.org/10.3390/ijms25105502 (registering DOI) - 17 May 2024
Abstract
Mitochondrial genomes of land plants are large and exhibit a complex mode of gene organization and expression, particularly at the post-transcriptional level. The primary organellar transcripts in plants undergo extensive maturation steps, including endo- and/or exo-nucleolytic cleavage, RNA-base modifications (mostly C-to-U [...] Read more.
Mitochondrial genomes of land plants are large and exhibit a complex mode of gene organization and expression, particularly at the post-transcriptional level. The primary organellar transcripts in plants undergo extensive maturation steps, including endo- and/or exo-nucleolytic cleavage, RNA-base modifications (mostly C-to-U deaminations) and both ‘cis’- and ‘trans’-splicing events. These essential processing steps rely on the activities of a large set of nuclear-encoded factors. RNA helicases serve as key players in RNA metabolism, participating in the regulation of transcription, mRNA processing and translation. They unwind RNA secondary structures and facilitate the formation of ribonucleoprotein complexes crucial for various stages of gene expression. Furthermore, RNA helicases are involved in RNA metabolism by modulating pre-mRNA maturation, transport and degradation processes. These enzymes are, therefore, pivotal in RNA quality-control mechanisms, ensuring the fidelity and efficiency of RNA processing and turnover in plant mitochondria. This review summarizes the significant roles played by helicases in regulating the highly dynamic processes of mitochondrial transcription, RNA processing and translation in plants. We further discuss recent advancements in understanding how dysregulation of mitochondrial RNA helicases affects the splicing of organellar genes, leading to respiratory dysfunctions, and consequently, altered growth, development and physiology of land plants. Full article
10 pages, 1556 KiB  
Article
Solvothermal Fabrication of Mesoporous Pd Nano-Corals at Mild Temperature for Alkaline Hydrogen Evolution Reaction
by Ming Zhao, Koh-ichi Maruyama and Satoshi Tanaka
Nanomaterials 2024, 14(10), 876; https://doi.org/10.3390/nano14100876 (registering DOI) - 17 May 2024
Abstract
Porous metallic nanomaterials exhibit interesting physical and chemical properties, and are widely used in various fields. Traditional fabrication techniques are limited to metallurgy, sintering, electrodeposition, etc., which limit the control of pore size and distribution, and make it difficult to achieve materials with [...] Read more.
Porous metallic nanomaterials exhibit interesting physical and chemical properties, and are widely used in various fields. Traditional fabrication techniques are limited to metallurgy, sintering, electrodeposition, etc., which limit the control of pore size and distribution, and make it difficult to achieve materials with high surface areas. On the other hand, the chemical preparation of metallic nanoparticles is usually carried out with strong reducing agents or at high temperature, resulting in the formation of dispersed particles which cannot evolve into porous metal. In this study, we reported the simple fabrication of coral-like mesoporous Pd nanomaterial (Pd NC) with a ligament size of 4.1 nm. The fabrication was carried out by simple solvothermal reduction at a mild temperature of 135 °C, without using any templates. The control experiments suggested that tetrabutylammonium bromide (TBAB) played a critical role in the Pd(II) reduction into Pd nanoclusters and their subsequent aggregation to form Pd NC, and another key point for the formation of Pd NC is not to use a strong reducing agent. In alkaline water electrolysis, the Pd NC outperforms the monodisperse Pd NPs and the state-of-the-art Pt (under large potentials) for H2 evolution reaction, probably due to its mesoporous structure and large surface area. This work reports a simple and novel method for producing porous metallic nanomaterials with a high utilization efficiency of metal atoms, and it is expected to contribute to the practical preparation of porous metallic nanomaterials by solvothermal reductions. Full article
24 pages, 1307 KiB  
Article
Bioaccessibility of Metallic Nickel and Nickel Oxide Nanoparticles in Four Simulated Biological Fluids
by Tara Lyons-Darden, Katherine E. Heim, Li Han, Laura Haines, Christie M. Sayes and Adriana R. Oller
Nanomaterials 2024, 14(10), 877; https://doi.org/10.3390/nano14100877 (registering DOI) - 17 May 2024
Abstract
Bioaccessibility of metals from substances and alloys is increasingly used as part of the assessment to predict potential toxicity. However, data are sparse on the metal bioaccessibility from nanoparticle (NP) size metal substances. This study examines nickel ion release from metallic nickel and [...] Read more.
Bioaccessibility of metals from substances and alloys is increasingly used as part of the assessment to predict potential toxicity. However, data are sparse on the metal bioaccessibility from nanoparticle (NP) size metal substances. This study examines nickel ion release from metallic nickel and nickel oxide micron particles (MPs) and NPs in simulated biological fluids at various timepoints including those relevant for specific routes of exposure. The results suggest that MPs of both metallic nickel and nickel oxide generally released more nickel ions in acidic simulated biological fluids (gastric and lysosomal) than NPs of the same substance, with the largest differences being for nickel oxide. In more neutral pH fluids (interstitial and perspiration), nickel metal NPs released more nickel ions than MPs, with nickel oxide results showing a higher release for MPs in interstitial fluid yet a lower release in perspiration fluid. Various experimental factors related to the particle, fluid, and extraction duration were identified that can have an impact on the particle dissolution and release of nickel ions. Overall, the results suggest that based on nickel release alone, nickel NPs are not inherently more hazardous than nickel MPs. Moreover, analyses should be performed on a case-by-case basis with consideration of various experimental factors and correlation with in vivo data. Full article
(This article belongs to the Section Biology and Medicines)
9 pages, 373 KiB  
Article
Serum Biochemical Parameters of Broilers Affected by Wooden Breast Myopathy
by Eglė Lebednikaitė, Neringa Sutkevičienė, Toma Vilkonienė, Žana Balčiauskienė, Kęstutis Kučinskas, Lina Anskienė and Alius Pockevičius
Animals 2024, 14(10), 1499; https://doi.org/10.3390/ani14101499 (registering DOI) - 17 May 2024
Abstract
Wooden breast (WB) myopathy is a pathology of the pectoralis major muscle. Wooden breast is caused by multiple factors. The exact etiopathogenesis of this myodegenerative pathology is still unclear. Fast-growing commercial lines of broilers that are selected for high breast muscle yields are [...] Read more.
Wooden breast (WB) myopathy is a pathology of the pectoralis major muscle. Wooden breast is caused by multiple factors. The exact etiopathogenesis of this myodegenerative pathology is still unclear. Fast-growing commercial lines of broilers that are selected for high breast muscle yields are more susceptible to this myopathy. The biochemical analysis of blood is used to diagnose pathologies and understand disease processes. Therefore, the objective of this research was to determine and compare the changes in the blood serum biochemical parameters of Ross 308 chicken broilers without myopathy and those affected by WB myopathy. Blood samples were collected from male and female Ross 308 broilers that were 43 days old, with an average live weight of 2.98–3.09 kg. Representative blood samples were selected from broilers with WB (n = 33) and without WB (n = 33). In the laboratory, the blood was centrifugated, and biochemical tests were performed with an automated computerized biochemistry analyzer. The research results showed that broilers with WB had elevated blood serum levels of creatine kinase (CK) (p = 0.018), potassium (p = 0.010), and alanine aminotransferase (ALT) (p = 0.012). In conclusion, elevated serum levels of CK and potassium indicated that skeletal muscle cells were damaged. Moreover, increased ALT levels suggested a possible association between WB myopathy and liver damage. Additionally, these research findings underscore the diagnostic significance of CK and hint at its potential as a WB biomarker. Full article
(This article belongs to the Section Poultry)
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39 pages, 1895 KiB  
Review
Comparing Redox and Intracellular Signalling Responses to Cold Plasma in Wound Healing and Cancer
by Adrian I. Abdo and Zlatko Kopecki
Curr. Issues Mol. Biol. 2024, 46(5), 4885-4923; https://doi.org/10.3390/cimb46050294 (registering DOI) - 17 May 2024
Abstract
Cold plasma (CP) is an ionised gas containing excited molecules and ions, radicals, and free electrons, and which emits electric fields and UV radiation. CP is potently antimicrobial, and can be applied safely to biological tissue, birthing the field of plasma medicine. Reactive [...] Read more.
Cold plasma (CP) is an ionised gas containing excited molecules and ions, radicals, and free electrons, and which emits electric fields and UV radiation. CP is potently antimicrobial, and can be applied safely to biological tissue, birthing the field of plasma medicine. Reactive oxygen and nitrogen species (RONS) produced by CP affect biological processes directly or indirectly via the modification of cellular lipids, proteins, DNA, and intracellular signalling pathways. CP can be applied at lower levels for oxidative eustress to activate cell proliferation, motility, migration, and antioxidant production in normal cells, mainly potentiated by the unfolded protein response, the nuclear factor-erythroid factor 2-related factor 2 (Nrf2)-activated antioxidant response element, and the phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) pathway, which also activates nuclear factor-kappa B (NFκB). At higher CP exposures, inactivation, apoptosis, and autophagy of malignant cells can occur via the degradation of the PI3K/Akt and mitogen-activated protein kinase (MAPK)-dependent and -independent activation of the master tumour suppressor p53, leading to caspase-mediated cell death. These opposing responses validate a hormesis approach to plasma medicine. Clinical applications of CP are becoming increasingly realised in wound healing, while clinical effectiveness in tumours is currently coming to light. This review will outline advances in plasma medicine and compare the main redox and intracellular signalling responses to CP in wound healing and cancer. Full article
(This article belongs to the Special Issue Molecular Research on Free Radicals and Oxidative Stress)
21 pages, 993 KiB  
Communication
The Crucial Role of Interdisciplinary Conferences in Advancing Explainable AI in Healthcare
by Ankush U. Patel, Qiangqiang Gu, Ronda Esper, Danielle Maeser and Nicole Maeser
BioMedInformatics 2024, 4(2), 1363-1383; https://doi.org/10.3390/biomedinformatics4020075 (registering DOI) - 17 May 2024
Abstract
As artificial intelligence (AI) integrates within the intersecting domains of healthcare and computational biology, developing interpretable models tailored to medical contexts is met with significant challenges. Explainable AI (XAI) is vital for fostering trust and enabling effective use of AI in healthcare, particularly [...] Read more.
As artificial intelligence (AI) integrates within the intersecting domains of healthcare and computational biology, developing interpretable models tailored to medical contexts is met with significant challenges. Explainable AI (XAI) is vital for fostering trust and enabling effective use of AI in healthcare, particularly in image-based specialties such as pathology and radiology where adjunctive AI solutions for diagnostic image analysis are increasingly utilized. Overcoming these challenges necessitates interdisciplinary collaboration, essential for advancing XAI to enhance patient care. This commentary underscores the critical role of interdisciplinary conferences in promoting the necessary cross-disciplinary exchange for XAI innovation. A literature review was conducted to identify key challenges, best practices, and case studies related to interdisciplinary collaboration for XAI in healthcare. The distinctive contributions of specialized conferences in fostering dialogue, driving innovation, and influencing research directions were scrutinized. Best practices and recommendations for fostering collaboration, organizing conferences, and achieving targeted XAI solutions were adapted from the literature. By enabling crucial collaborative junctures that drive XAI progress, interdisciplinary conferences integrate diverse insights to produce new ideas, identify knowledge gaps, crystallize solutions, and spur long-term partnerships that generate high-impact research. Thoughtful structuring of these events, such as including sessions focused on theoretical foundations, real-world applications, and standardized evaluation, along with ample networking opportunities, is key to directing varied expertise toward overcoming core challenges. Successful collaborations depend on building mutual understanding and respect, clear communication, defined roles, and a shared commitment to the ethical development of robust, interpretable models. Specialized conferences are essential to shape the future of explainable AI and computational biology, contributing to improved patient outcomes and healthcare innovations. Recognizing the catalytic power of this collaborative model is key to accelerating the innovation and implementation of interpretable AI in medicine. Full article
(This article belongs to the Topic Computational Intelligence and Bioinformatics (CIB))
25 pages, 666 KiB  
Article
New Bivariate Copulas via Lomax Distribution Generated Distortions
by Fadal Abdullah Ali Aldhufairi and Jungsywan H. Sepanski
AppliedMath 2024, 4(2), 641-665; https://doi.org/10.3390/appliedmath4020035 (registering DOI) - 17 May 2024
Abstract
We develop a framework for creating distortion functions that are used to construct new bivariate copulas. It is achieved by transforming non-negative random variables with Lomax-related distributions. In this paper, we apply the distortions to the base copulas of independence, Clayton, Frank, and [...] Read more.
We develop a framework for creating distortion functions that are used to construct new bivariate copulas. It is achieved by transforming non-negative random variables with Lomax-related distributions. In this paper, we apply the distortions to the base copulas of independence, Clayton, Frank, and Gumbel copulas. The properties of the tail dependence coefficient, tail order, and concordance ordering are explored for the new families of distorted copulas. We conducted an empirical study using the daily net returns of Amazon and Google stocks from January 2014 to December 2023. We compared the popular Clayton, Gumbel, Frank, and Gaussian copula models to their corresponding distorted copula models induced by the unit-Lomax and unit-inverse Pareto distortions. The new families of distortion copulas are equipped with additional parameters inherent in the distortion function, providing more flexibility, and are demonstrated to perform better than the base copulas. After analyzing the data, we have found that the joint extremes of Amazon and Google stocks are more likely for high daily net returns than for low daily net returns. Full article
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20 pages, 332 KiB  
Article
Constructive Journalistic Roles in Environments of Social Complexity and Political Crisis
by Alfredo Rojas-Calderón
Journal. Media 2024, 5(2), 626-645; https://doi.org/10.3390/journalmedia5020042 (registering DOI) - 17 May 2024
Abstract
This exploratory and analytical research examines secondary sources to propose a résumé of professional roles for journalists to revitalize their roles within social and political coexistence. It aims to bridge the gap between theoretical concepts and practical applications, enhancing journalists’ impact on public [...] Read more.
This exploratory and analytical research examines secondary sources to propose a résumé of professional roles for journalists to revitalize their roles within social and political coexistence. It aims to bridge the gap between theoretical concepts and practical applications, enhancing journalists’ impact on public discourse and informed decision-making. Empirical research will guide these roles’ implementation in real-world journalistic practices. Twelve explanatory and constructive journalistic roles are formulated, whose relevance and application are enhanced in societies undergoing crisis situations or serious difficulties and are at risk of rupture in coexistence. In conclusion, the role of journalists in the current circumstances of crises in democratic societies requires them to not only report on events but also provide context, analysis, and solutions to the complex issues faced by society. Journalistic functions such as contextualization, public interest promotion, dialogue, and motivation are considered fundamental beyond constructive journalism or solutions journalism. Full article
(This article belongs to the Special Issue New Roles of Journalism and Disruptive Media: A Challenging Future)
14 pages, 1063 KiB  
Article
Assessment of Cognitive Function in Romanian Patients with Chronic Alcohol Consumption
by Shandiz Morega, Claudiu-Marinel Ionele, Mihaela-Andreea Podeanu, Dan-Nicolae Florescu and Ion Rogoveanu
Gastroenterol. Insights 2024, 15(2), 433-446; https://doi.org/10.3390/gastroent15020031 (registering DOI) - 17 May 2024
Abstract
Alcoholism presents a significant health concern with notable socioeconomic implications. Alcohol withdrawal syndrome (AWS) can manifest when individuals cease or drastically reduce their alcohol consumption after prolonged use. Non-alcoholic fatty liver disease (NAFLD) is characterized by substantial lipid accumulation in the liver cells [...] Read more.
Alcoholism presents a significant health concern with notable socioeconomic implications. Alcohol withdrawal syndrome (AWS) can manifest when individuals cease or drastically reduce their alcohol consumption after prolonged use. Non-alcoholic fatty liver disease (NAFLD) is characterized by substantial lipid accumulation in the liver cells of individuals with no history of alcohol consumption. There is evidence suggesting an association between cognitive impairment and both conditions. This study aimed to evaluate cognitive impairment in patients with NAFLD and AWS using the Mini-Mental State Examination (MMSE). This study involved 120 patients admitted to two hospitals in Craiova, Romania. Results indicated that patients with NAFLD did not exhibit cognitive impairment as measured by MMSE (Mean = 29.27, SD = 0.785). Conversely, patients with AWS showed more pronounced cognitive dysfunction, with a mean MMSE score at admission of 16.60 ± 4.097 and 24.60 ± 2.832 after 2 weeks under treatment with Vitamins B1 and B6 and Cerebrolysin. Additionally, our findings suggested that cognitive dysfunction among alcohol consumers was correlated with the severity of clinical symptoms, as demonstrated by the severity of tremors in our study. The two-week period under treatment and alcohol withdrawal was insufficient for cognitive function to return to normal levels. Observational studies on longer periods of time are advised. Full article
(This article belongs to the Special Issue Novelties in Diagnostics and Therapeutics in Hepatology: 2nd Edition)
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16 pages, 1070 KiB  
Article
Density and Composition of Cohabiting Bacteria in Chlorella vulgaris CCAP 211/21A is Influenced by Changes in Nutrient Supply
by Wasayf J Almalki, Alison O. Nwokeoji and Seetharaman Vaidyanathan
SynBio 2024, 2(2), 190-205; https://doi.org/10.3390/synbio2020011 (registering DOI) - 17 May 2024
Abstract
Microalgae have considerable potential as a renewable feedstock for biochemical and bioethanol production that can be employed in processes associated with carbon capture. Large-scale microalgae cultivations are often non-axenic and are often cohabited by bacteria. A better understanding of the influence of cohabiting [...] Read more.
Microalgae have considerable potential as a renewable feedstock for biochemical and bioethanol production that can be employed in processes associated with carbon capture. Large-scale microalgae cultivations are often non-axenic and are often cohabited by bacteria. A better understanding of the influence of cohabiting bacteria on microalgae productivity is required to develop sustainable synthetic co-culture processes at scale. Nutrient limitation is a frequently employed strategy in algal cultivations to accumulate energy reserves, such as lipids and carbohydrates. Here, a non-axenic culture of an estuarine green microalga, Chlorella vulgaris CCAP 211/21A, was studied under nutrient replete and deplete conditions to assess how changes in nutrient supply influenced the cohabiting bacterial population and its association with intracellular carbohydrate accumulations in the alga. Nutrient limitation resulted in a maximum carbohydrate yield of 47%, which was 74% higher than that in nutrient replete conditions. However, the latter condition elicited a 2-fold higher carbohydrate productivity. Three cohabiting bacterial isolates were cultivable from the three culture conditions tested. These isolates were identified using the 16S rRNA gene sequence to belong to Halomonas sp. and Muricauda sp. The composition of the bacterial population varied significantly between the growth conditions and time points. In all cases and at all time points, the dominant species was Halomonas isolates. Nutrient depletion resulted in an apparent loss of Muricauda sp. This finding demonstrates that nutrient supply can be used to control cohabiting bacterial populations in algal cultures, which will enable the development of synthetic co-culture strategies for improving algae productivity. Full article
34 pages, 22821 KiB  
Article
An Innovative System for Enhancing Archaeological In Situ Excavation through Geospatial Integration
by Asimina Dimara, Sotirios Tsakiridis, Doukas Psarros, Alexios Papaioannou, Dimitrios Varsamis, Christos-Nikolaos Anagnostopoulos and Stelios Krinidis
Heritage 2024, 7(5), 2586-2619; https://doi.org/10.3390/heritage7050124 (registering DOI) - 17 May 2024
Abstract
The field of archaeological excavation has seen enormous developments as a result of the quick development of digital technologies. It is critical to acknowledge the long-term benefits of new approaches. In this regard, this study intends to suggest a system that provides archaeologists [...] Read more.
The field of archaeological excavation has seen enormous developments as a result of the quick development of digital technologies. It is critical to acknowledge the long-term benefits of new approaches. In this regard, this study intends to suggest a system that provides archaeologists with digital tools that allow them to fully and effectively document their excavations in real time while in the field, which is specifically designed for classical and Byzantine archaeology. The system not only facilitates traditional documentation practices but also integrates advanced geospatial technologies and augmented reality, enhancing the accuracy and depth of archaeological research and preservation. This dual functionality enables both the efficient recording of excavation data as experienced by excavators on-site and the detailed documentation processed by researchers post-excavation. The objective of the application is to generate a sophisticated system that will enable the excavation data and experience that the excavator gains on the site of work to be recorded in real time, as well as the documentation that is subsequently processed and accomplished by the researcher or by other researchers. The system saves detailed images and 3D models of artifacts and excavation sites. This ensures that every detail is recorded while preserving the data for future analysis and reference. Lastly, the application was tested in realistic use case scenarios and real-world settings, which increased the system’s credibility and demonstrated its capability to enhance the procedures involved in archaeological excavation and documentation. Full article
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19 pages, 578 KiB  
Article
Training a Filter-Based Model of the Cochlea in the Context of Pre-Trained Acoustic Models
by Louise Coppieters de Gibson and Philip N. Garner
Acoustics 2024, 6(2), 470-488; https://doi.org/10.3390/acoustics6020025 (registering DOI) - 17 May 2024
Abstract
Auditory research aims in general to lead to understanding of physiological processes. By contrast, the state of the art in automatic speech processing (notably recognition) is dominated by large pre-trained models that are meant to be used as black-boxes. In this work, we [...] Read more.
Auditory research aims in general to lead to understanding of physiological processes. By contrast, the state of the art in automatic speech processing (notably recognition) is dominated by large pre-trained models that are meant to be used as black-boxes. In this work, we integrate a physiologically plausible (albeit simple filter-based) model of the cochlea into a much larger pre-trained acoustic model for speech recognition. We show that the hybrid system can be trained and evaluated with various combinations of fine-tuning and self-supervision. The results broadly show that the system automatically yields structures that are known to work well. Moreover, these structures lack artifacts that were apparent in (our) previous work using less sophisticated neural models. We conclude that the hybrid structure is an appropriate way to proceed in auditory research, more generally allowing the work to take advantage of larger models and databases from which it would not otherwise benefit. Full article
14 pages, 539 KiB  
Article
Arterial Stiffness Is an Important Predictor of Heart Failure with Preserved Ejection Fraction (HFpEF)—The Effects of Phosphate Retention
by Yuji Mizuno, Toshifumi Ishida, Kenichi Tsujita and Michihiro Yoshimura
Hearts 2024, 5(2), 211-224; https://doi.org/10.3390/hearts5020014 (registering DOI) - 17 May 2024
Abstract
Heart failure with preserved ejection fraction (HFpEF) is a major health concern. There is a growing recognition of the causal interplay between arterial stiffness and HFpEF. We recently reported that phosphate retention is a trigger for arterial stiffness. This study focuses on whether [...] Read more.
Heart failure with preserved ejection fraction (HFpEF) is a major health concern. There is a growing recognition of the causal interplay between arterial stiffness and HFpEF. We recently reported that phosphate retention is a trigger for arterial stiffness. This study focuses on whether arterial stiffness due to phosphate retention could be a predictor for HFpEF. Methods: The subjects of this study were 158 patients (68 males and 90 females, mean age 74.8 ± 11.2). HFpEF was defined according to the guidelines of the ESC 2019. Pulse wave velocity (PWV) and central systolic blood pressure (CSBP) were used as markers for arterial stiffness and afterload, respectively. We measured serum levels of fibroblast growth factor 23 (FGF23) as a marker of phosphate retention. Results: The serum levels of FGF23 had a significant relationship with PWV. PWV had significant relationships with LV mass index, plasma BNP levels, and relative wall thickness, e’, and E/e’ (p < 0.001, respectively). Multivariate logistic regression analysis revealed that higher PWV values and hypertension were significant predictors for the dependent factor (HFpEF). Arterial stiffness amplified afterload, leading to LV concentric hypertrophy and diastolic dysfunction. This study presents that arterial stiffness is a key predictor of HFpEF, and that phosphate retention is involved in the pathology of HFpEF. Full article
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15 pages, 637 KiB  
Article
Incorporating Symbolic Discrete Controller Synthesis into a Virtual Robot Experimental Platform: An Implementation with Collaborative Unmanned Aerial Vehicle Robots
by Mete Özbaltan and Serkan Çaşka
Drones 2024, 8(5), 206; https://doi.org/10.3390/drones8050206 (registering DOI) - 17 May 2024
Abstract
We introduce a modeling framework aimed at incorporating symbolic discrete controller synthesis (DCS) into a virtual robot experimental platform. This framework involves symbolically representing the behaviors of robotic systems along with their control objectives using synchronous programming techniques. We employed DCS algorithms through [...] Read more.
We introduce a modeling framework aimed at incorporating symbolic discrete controller synthesis (DCS) into a virtual robot experimental platform. This framework involves symbolically representing the behaviors of robotic systems along with their control objectives using synchronous programming techniques. We employed DCS algorithms through the reactive synchronous environment ReaX to generate controllers that fulfill specified objectives. These resulting controllers were subsequently deployed on the virtual robot experimental platform Simscape. To demonstrate and validate our approach, we provide an implementation example involving collaborative UAV robots. Full article
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11 pages, 1490 KiB  
Article
The Relationship between the Ewing Test, Sudoscan Cardiovascular Autonomic Neuropathy Score and Cardiovascular Risk Score Calculated with SCORE2-Diabetes
by Andra-Elena Nica, Emilia Rusu, Carmen Dobjanschi, Florin Rusu, Claudia Sivu, Oana Andreea Parlițeanu and Gabriela Radulian
Medicina 2024, 60(5), 828; https://doi.org/10.3390/medicina60050828 (registering DOI) - 17 May 2024
Abstract
Background and Objectives: Cardiac autonomic neuropathy (CAN) is a severe complication of diabetes mellitus (DM) strongly linked to a nearly five-fold higher risk of cardiovascular mortality. Patients with Type 2 Diabetes Mellitus (T2DM) are a significant cohort in which these assessments have [...] Read more.
Background and Objectives: Cardiac autonomic neuropathy (CAN) is a severe complication of diabetes mellitus (DM) strongly linked to a nearly five-fold higher risk of cardiovascular mortality. Patients with Type 2 Diabetes Mellitus (T2DM) are a significant cohort in which these assessments have particular relevance to the increased cardiovascular risk inherent in the condition. Materials and Methods: This study aimed to explore the subtle correlation between the Ewing test, Sudoscan-cardiovascular autonomic neuropathy score, and cardiovascular risk calculated using SCORE 2 Diabetes in individuals with T2DM. The methodology involved detailed assessments including Sudoscan tests to evaluate sudomotor function and various cardiovascular reflex tests (CART). The cohort consisted of 211 patients diagnosed with T2DM with overweight or obesity without established ASCVD, aged between 40 to 69 years. Results: The prevalence of CAN in our group was 67.2%. In the study group, according SCORE2-Diabetes, four patients (1.9%) were classified with moderate cardiovascular risk, thirty-five (16.6%) with high risk, and one hundred seventy-two (81.5%) with very high cardiovascular risk. Conclusions: On multiple linear regression, the SCORE2-Diabetes algorithm remained significantly associated with Sudoscan CAN-score and Sudoscan Nephro-score and Ewing test score. Testing for the diagnosis of CAN in very high-risk patients should be performed because approximately 70% of them associate CAN. Increased cardiovascular risk is associated with sudomotor damage and that Sudoscan is an effective and non-invasive measure of identifying such risk. Full article
(This article belongs to the Special Issue Advances in Clinical Diabetes, Obesity, and Metabolic Diseases)
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15 pages, 563 KiB  
Article
Advancements in Battery Cell Finalization: Insights from an Expert Survey and Prospects for Process Optimization
by Tobias Robben, Christian Offermanns, Heiner Heimes and Achim Kampker
World Electr. Veh. J. 2024, 15(5), 219; https://doi.org/10.3390/wevj15050219 (registering DOI) - 17 May 2024
Abstract
Battery cell finalization is a crucial process chain in battery manufacturing, contributing to a significant share of CAPEX and OPEX. Thus, there is a high cost-saving potential by improving the process chain. This research paper investigates various crucial facets of the cell finalization [...] Read more.
Battery cell finalization is a crucial process chain in battery manufacturing, contributing to a significant share of CAPEX and OPEX. Thus, there is a high cost-saving potential by improving the process chain. This research paper investigates various crucial facets of the cell finalization process in battery cell production through an expert survey. These include investment cost allocation, potential cost savings in sub-processes, reject generation, early detection of faulty cells, quality measurement techniques, and the utilization of inline data for early quality determination and real-time process control during the formation process. A solution approach for the implementation of electrochemical impedance spectroscopy for inline early quality determination is given. The results yield valuable insights for optimizing the formation process and enhancing product quality. Full article
23 pages, 6416 KiB  
Article
Analysis of Scalable Resonant DC–DC Converter Using GaN Switches for xEV Charging Stations
by Rajanand Patnaik Narasipuram, Subbarao Mopidevi, Anton Dianov and Amit Singh Tandon
World Electr. Veh. J. 2024, 15(5), 218; https://doi.org/10.3390/wevj15050218 (registering DOI) - 17 May 2024
Abstract
In this research, an innovative electric vehicle (EV) charger is designed and presented for xEV charging stations. The key feature of our system is a scalable, interleaved inductor–inductor–capacitor (iL2C) DC-DC converter operation. The proposed system employs two parallel L2C [...] Read more.
In this research, an innovative electric vehicle (EV) charger is designed and presented for xEV charging stations. The key feature of our system is a scalable, interleaved inductor–inductor–capacitor (iL2C) DC-DC converter operation. The proposed system employs two parallel L2C converters with 8-GaN switches on the primary side and a shared rectifier circuit on the secondary side. This configuration not only amplifies the resonant tank internal currents and losses generated by the switches but also improves current sharing. A novel closed-loop technique is proposed with a constant-voltage method of operation, along with a hybrid control scheme of variable frequency + phase shift modulation (VFPSM). To examine the controller and converter’s performance, an experimental demonstration is conducted under varying load conditions, including full load, half load, and light load, where the source voltage and load voltage are maintained at constant levels of 400 Vin and 48 V0, respectively. Furthermore, line regulation is conducted and verified to accommodate a broad input voltage range of 300 Vin–500 Vin and 500 Vin–300 Vin while maintaining an output voltage of 48 V0 at 3.3 kW, 1.65 kW, and 0.33 kW with a peak efficiency of 98.2%. Full article
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16 pages, 2695 KiB  
Article
Healing of Periodontal Suprabony Defects following Treatment with Open Flap Debridement with or without Hyaluronic Acid (HA) Application
by Octavia Carolina Vela, Marius Boariu, Darian Rusu, Vincenzo Iorio-Siciliano, Luca Ramaglia, Simina Boia, Viorelia Radulescu, Ioana Ilyes and Stefan-Ioan Stratul
Medicina 2024, 60(5), 829; https://doi.org/10.3390/medicina60050829 (registering DOI) - 17 May 2024
Abstract
Background and Objectives: This randomized, double-arm, multicentric clinical trial aims to compare the clinical outcomes following the treatment of suprabony periodontal defects using open flap debridement (OFD) with or without the application of hyaluronic acid (HA). Materials and Methods: Sixty systemically healthy patients [...] Read more.
Background and Objectives: This randomized, double-arm, multicentric clinical trial aims to compare the clinical outcomes following the treatment of suprabony periodontal defects using open flap debridement (OFD) with or without the application of hyaluronic acid (HA). Materials and Methods: Sixty systemically healthy patients with at least two teeth presenting suprabony periodontal defects were randomly assigned with a 1:1 allocation ratio using computer-generated tables into a test (OFD + HA) or control group (OFD). The main outcome variable was clinical attachment level (CAL). The secondary outcome variables were changes in mean probing pocket depth (PPD), gingival recession (GR), full-mouth plaque score (FMPS), and full-mouth bleeding score (FMBS). All clinical measurements were carried out at baseline and 12 months. Results: Sixty patients, thirty in each group, were available for statistical analysis. The mean CAL gain was statistically significantly different (p < 0.001) in the test group compared with the control group (3.06 ± 1.13 mm vs. 1.44 ± 1.07 mm). PPD reduction of test group measurements (3.28 ± 1.14 mm) versus the control group measurements (2.61 ± 1.22 mm) were statistically significant (p = 0.032). GR changes were statistically significant only in the test group 0.74 ± 1.03 mm (p < 0.001). FMBS and FMPS revealed a statistically significant improvement mostly in the test group. Conclusions: Suprabony periodontal defects could benefit from the additional application of HA in conjunction with OFD in terms of improvement of the clinical parameters compared with OFD alone. Full article
(This article belongs to the Special Issue Advances in Clinical Periodontology)
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22 pages, 604 KiB  
Article
XplAInable: Explainable AI Smoke Detection at the Edge
by Alexander Lehnert, Falko Gawantka, Jonas During, Franz Just and Marc Reichenbach
Big Data Cogn. Comput. 2024, 8(5), 50; https://doi.org/10.3390/bdcc8050050 (registering DOI) - 17 May 2024
Abstract
Wild and forest fires pose a threat to forests and thereby, in extension, to wild life and humanity. Recent history shows an increase in devastating damages caused by fires. Traditional fire detection systems, such as video surveillance, fail in the early stages of [...] Read more.
Wild and forest fires pose a threat to forests and thereby, in extension, to wild life and humanity. Recent history shows an increase in devastating damages caused by fires. Traditional fire detection systems, such as video surveillance, fail in the early stages of a rural forest fire. Such systems would see the fire only when the damage is immense. Novel low-power smoke detection units based on gas sensors can detect smoke fumes in the early development stages of fires. The required proximity is only achieved using a distributed network of sensors interconnected via 5G. In the context of battery-powered sensor nodes, energy efficiency becomes a key metric. Using AI classification combined with XAI enables improved confidence regarding measurements. In this work, we present both a low-power gas sensor for smoke detection and a system elaboration regarding energy-efficient communication schemes and XAI-based evaluation. We show that leveraging edge processing in a smart way combined with buffered data samples in a 5G communication network yields optimal energy efficiency and rating results. Full article
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11 pages, 438 KiB  
Article
Factors Affecting Adherence to Social Distancing among Adults Aged 19–44 Years: Insights from a Nationwide Survey during COVID-19 Pandemic
by Eun Jung Kim and Mikyong Byun
Medicina 2024, 60(5), 827; https://doi.org/10.3390/medicina60050827 (registering DOI) - 17 May 2024
Abstract
Background and Objectives: Before COVID-19 vaccinations became available, adhering to non-pharmaceutical interventions (NPIs), like social distancing (SD), wearing masks, and hand hygiene, were crucial to mitigating viral spread. Many studies reported that younger individuals were more reluctant to follow these measures compared with [...] Read more.
Background and Objectives: Before COVID-19 vaccinations became available, adhering to non-pharmaceutical interventions (NPIs), like social distancing (SD), wearing masks, and hand hygiene, were crucial to mitigating viral spread. Many studies reported that younger individuals were more reluctant to follow these measures compared with older ones. We hypothesized that it would be worthwhile to find factors that influenced SD compliance among young people during the pre-vaccination phase of a pandemic. Materials and Methods: We analyzed data of adults aged 19–44 from the 2020 South Korean Community Health Survey and compared socio-demographic, health-related behavioral, and psychological factors between compliant and non-compliant cohorts. Results: A total of 59,943 participants were enrolled and we found that older age groups (30–39 and 40–44) and safety concerns (such as viral infection, virus-related death, economic damage, and transmitting virus to vulnerable people) were significantly associated with adherence to SD. Conversely, participants who were not living with a spouse, were unable to stay at home despite symptoms, smoked, drank, and had a negative attitude toward government policy statistically correlated with non-compliance. Conclusions: In times when NPIs were the primary defense against the pandemic, it is essential to identify factors that positively or negatively affect individual compliance with them, especially among young people. Using a large-scale, well-designed national survey, we could gain insights into the early recognition of risk factors for non-compliance and appropriate follow-up interventions (i.e., education campaigns, clear communication of public guidelines, and implementation of guidelines), which will help people to avoid suffering from other waves of future infectious diseases. Full article
10 pages, 1279 KiB  
Case Report
Cost-Effectiveness in Alternative Treatment Options for Pancreatic Pseudocysts
by Nikola Boyanov, Nikol Milinich, Katina Shtereva, Katerina Madzharova, Stoilka Tufkova, Mariana Penkova-Radicheva, Daniela Radicheva and Neno Shopov
Reports 2024, 7(2), 38; https://doi.org/10.3390/reports7020038 (registering DOI) - 17 May 2024
Abstract
Pancreatic pseudocysts often arise as complications of pancreatitis and present unique challenges in clinical management, encompassing considerations for both technical aspects and financial implications. Before the advancements of invasive gastroenterology, pancreatic pseudocysts have been drained surgically for many years. Nowadays, we have less [...] Read more.
Pancreatic pseudocysts often arise as complications of pancreatitis and present unique challenges in clinical management, encompassing considerations for both technical aspects and financial implications. Before the advancements of invasive gastroenterology, pancreatic pseudocysts have been drained surgically for many years. Nowadays, we have less invasive techniques with higher efficiency and lower mortality rates, however, they remain cost-challenging for most countries. Materials and Methods: We present four patients (two males and two females) with pancreatic pseudocysts who underwent endoscopic ultrasound-guided transgastric drainage using plastic stents accompanied by a standard lavage protocol using a nasocystic catheter. Results: All four patients had successful outcomes, and a follow-up at 6 months revealed no traces of the pseudocysts or any significant long-term complications. One acute complication (arterial bleeding) and one late complication (stent migration) were observed. As the study aimed to present a cheaper option for draining pancreatic pseudocysts, we investigated and compared costs for the materials we utilized and those associated with lumen-apposing metal stents. Upon compiling the data, a notable advantage was evident in favour of our method. Conclusions: While EUS-guided drainage of pancreatic pseudocysts using lumen-apposing metal stents (LAMSs) represents a high-end strategy for treating pancreatic pseudocysts, our method demonstrates better cost-effectiveness without compromising efficacy. Full article
19 pages, 4411 KiB  
Article
Polyphenols from Sage Leaves (Salvia officinalis L.): Environmentally Friendly Extraction under High Hydrostatic Pressure and Application as a Corrosion Inhibitor for Tinplate
by Maja Dent, Regina Fuchs-Godec, Sandra Pedisić, Dorotea Grbin, Verica Dragović-Uzelac, Damir Ježek and Tomislav Bosiljkov
Separations 2024, 11(5), 158; https://doi.org/10.3390/separations11050158 (registering DOI) - 17 May 2024
Abstract
Due to the diversity of organic molecular structures present in sage extract, sage extract is a promising potential source of a cheap and effective biodegradable green corrosion inhibitor for tinplate in 3% NaCl solution, which was evaluated in this study. HHP proved to [...] Read more.
Due to the diversity of organic molecular structures present in sage extract, sage extract is a promising potential source of a cheap and effective biodegradable green corrosion inhibitor for tinplate in 3% NaCl solution, which was evaluated in this study. HHP proved to be a new and emerging technology for the useful extraction of polyphenols from sage as a functional ingredient from natural sources. Analysis of variance among all tested independent factors (ethanol concentration, HHP parameters and temperature) revealed significant differences (p < 0.05) in total polyphenol content as well as for rosmarinic acid as the major phenolic compound in sage extract, while extraction time had no effect (p ˃ 0.05). The optimum HHP conditions (600 MPa, 30% ethanol, 60 °C and 5 min) gave a maximum extraction yield of total polyphenols of 3811.84 mg/100 g. Sage-leaf extracts were found to be a mixture of phenolic acids, namely rosmarinic and salvianolic acid K, epicatechin and luteolin-7-O-glucuronide glycoside. The corrosion results show that the sage extract at a concentration of 0.6 g/L in 3% NaCl is an effective corrosion inhibitor (93%), forming a passivation layer of sage extract consisting of organic compounds such as polyphenols on the surface of tinplate. Full article
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12 pages, 851 KiB  
Article
Growth Responses of Holcus lanatus L. (Velvet Grass) in Soils Contaminated with Cesium or Strontium
by Bayezid M. Khan, M. Ferdous Alam, Zinnat A. Begum and Ismail M. M. Rahman
Soil Syst. 2024, 8(2), 57; https://doi.org/10.3390/soilsystems8020057 (registering DOI) - 17 May 2024
Abstract
Radiocesium (r-Cs) and radiostrontium (r-Sr) released from nuclear accidents (e.g., Chornobyl, Fukushima) and routine operations (reactors, reprocessing) pose environmental and health concerns. Their primary pathway to humans is through plant uptake and subsequent bioaccumulation within the food chain. While soil amendments with potassium [...] Read more.
Radiocesium (r-Cs) and radiostrontium (r-Sr) released from nuclear accidents (e.g., Chornobyl, Fukushima) and routine operations (reactors, reprocessing) pose environmental and health concerns. Their primary pathway to humans is through plant uptake and subsequent bioaccumulation within the food chain. While soil amendments with potassium (K) and calcium (Ca) are known to mitigate r-Cs and r-Sr uptake, respectively, the impact on plant growth remains unclear. This study investigates the effects of Cs and Sr on the growth of Holcus lanatus L. seedlings under hydroponic and soil conditions with varying Cs and Sr concentrations. Stable isotopes of Cs and Sr served as non-radioactive analogs. Seedling growth was assessed across a range of Cs and Sr concentrations (≤1 and ≥4 mg L⁻¹). The impact of the addition of K and Ca on Cs/Sr uptake in amended soils was also evaluated. Additionally, this study examined how Cs and Sr amendments affected the influx rates of other nutrients in H. lanatus. Higher Cs and Sr concentrations (≥4 mg L⁻¹) significantly inhibited seedling growth, while lower concentrations had no effect. Notably, H. lanatus exhibited moderate Cs tolerance and strong Sr tolerance. Furthermore, K and Ca supplementation in Cs/Sr-amended soils demonstrably reduced plant uptake of these elements. This study also observed alterations in the uptake rates of other nutrients within H. lanatus due to Cs/Sr addition. This study suggests that H. lanatus exhibits moderate tolerance to Cs and Sr contamination, potentially making it suitable for revegetation efforts in contaminated grasslands. Additionally, K and Ca amendments show promise as a strategy to mitigate plant uptake of these radioisotopes further. These findings contribute to the development of safer revitalization strategies for areas impacted by nuclear accidents. Full article

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