The 2023 MDPI Annual Report has
been released!
 
19 pages, 3595 KiB  
Article
Four New Species and a New Combination of Boletaceae (Boletales) from Subtropical and Tropical China
by Rou Xue, Lin-Jie Su, Tai-Jie Yu, Chang Xu, Hong-Yan Huang, Nian-Kai Zeng, Guo-Li Zhang and Li-Ping Tang
J. Fungi 2024, 10(5), 348; https://doi.org/10.3390/jof10050348 (registering DOI) - 13 May 2024
Abstract
Previous studies have shown that boletes are abundant and diverse in China, especially in tropical and subtropical regions. In the present study, morphological, ecological, host relationship, and a four-locus (28S, tef1, rpb1, and rpb2) molecular phylogenetic analyses were used to [...] Read more.
Previous studies have shown that boletes are abundant and diverse in China, especially in tropical and subtropical regions. In the present study, morphological, ecological, host relationship, and a four-locus (28S, tef1, rpb1, and rpb2) molecular phylogenetic analyses were used to study the family Boletaceae in subtropical and tropical China. Four new bluing species are described from three genera, viz. Boletellus verruculosus (Chinese name疣柄条孢牛肝菌), Xerocomellus tenuis (Chinese name细柄红绒盖牛肝菌), Xer. brunneus (Chinese name褐盖红绒盖牛肝菌), and Xerocomus zhangii (Chinese name张氏绒盖牛肝菌). Moreover, the genus Nigroboletus is treated as a synonym of Xerocomellus, and a new combination, namely Xer. roseonigrescens (Chinese name玫瑰红绒盖牛肝菌), is proposed. Full article
(This article belongs to the Special Issue Taxonomy, Systematics and Evolution of Forestry Fungi, 2nd Edition)
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35 pages, 7529 KiB  
Review
Heat Transfer Enhancements Assessment in Hot Water Generation with Phase Change Materials (PCMs): A Review
by Diana Isabel Berrocal, Juan Blandon Rodriguez, Maria De Los Angeles Ortega Del Rosario, Itamar Harris and Arthur M. James Rivas
Energies 2024, 17(10), 2350; https://doi.org/10.3390/en17102350 (registering DOI) - 13 May 2024
Abstract
The utilization of phase change materials (PCMs) in solar water heating systems (SWHS) has undergone notable advancements, driven by a rising demand for systems delivering superior performance and efficiency. Extensive research suggests that enhancing heat transfer (HTE) in storage systems is crucial for [...] Read more.
The utilization of phase change materials (PCMs) in solar water heating systems (SWHS) has undergone notable advancements, driven by a rising demand for systems delivering superior performance and efficiency. Extensive research suggests that enhancing heat transfer (HTE) in storage systems is crucial for achieving these improvements. This review employs a bibliometric analysis to track the evolution of HTE methods within this field. While current literature underscores the necessity for further exploration into hot water generation applications, several methodologies exhibit significant promise. Particularly, strategies such as fins, encapsulation, and porous media emerge as prominent HTE techniques, alongside nanofluids, which hold the potential for augmenting solar water heating systems. This review also identifies numerous unexplored techniques awaiting investigation, aiming to pave new paths in research and application within the field of hot water generation. It highlights methods that could be used independently or alongside predominantly used techniques. Full article
(This article belongs to the Section J: Thermal Management)
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13 pages, 3528 KiB  
Article
Genome Mining of Fungal Unique Trichodiene Synthase-like Sesquiterpene Synthases
by Zhanren Cong, Qiang Yin, Kunhong Tian, Njeru Joe Mukoma, Liming Ouyang, Tom Hsiang, Lixin Zhang, Lan Jiang and Xueting Liu
J. Fungi 2024, 10(5), 350; https://doi.org/10.3390/jof10050350 (registering DOI) - 13 May 2024
Abstract
Sesquiterpenoids served as an important source for natural product drug discovery. Although genome mining approaches have revealed numerous novel sesquiterpenoids and biosynthetic enzymes, the comprehensive landscape of fungal sesquiterpene synthases (STSs) remains elusive. In this study, 123 previously reported fungal STSs were subjected [...] Read more.
Sesquiterpenoids served as an important source for natural product drug discovery. Although genome mining approaches have revealed numerous novel sesquiterpenoids and biosynthetic enzymes, the comprehensive landscape of fungal sesquiterpene synthases (STSs) remains elusive. In this study, 123 previously reported fungal STSs were subjected to phylogenetic analysis, resulting in the identification of a fungi-specific STS family known as trichodiene synthase-like sesquiterpene synthases (TDTSs). Subsequently, the application of hidden Markov models allowed the discovery of 517 TDTSs from our in-house fungi genome library of over 400 sequenced genomes, and these TDTSs were defined into 79 families based on a sequence similarity network. Based on the novelty of protein sequences and the completeness of their biosynthetic gene clusters, 23 TDTS genes were selected for heterologous expression in Aspergillus oryzae. In total, 10 TDTSs were active and collectively produced 12 mono- and sesquiterpenes, resulting in the identification of the first chamipinene synthase, as well as the first fungi-derived cedrene, sabinene, and camphene synthases. Additionally, with the guidance of functionally characterized TDTSs, we found that TDTSs in Family 1 could produce bridged-cyclic sesquiterpenes, while those in Family 2 could synthesize spiro- and bridged-cyclic sesquiterpenes. Our research presents a new avenue for the genome mining of fungal sesquiterpenoids. Full article
(This article belongs to the Section Fungal Genomics, Genetics and Molecular Biology)
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22 pages, 2359 KiB  
Article
Advancing Cycling Safety: On-Bike Alert System Utilizing Multi-Layer Radar Point Cloud Clustering for Coarse Object Classification
by Asma Omri, Noureddine Benothman, Sofiane Sayahi, Fethi Tlili, Ferdaous Chaabane and Hichem Besbes
Sensors 2024, 24(10), 3094; https://doi.org/10.3390/s24103094 (registering DOI) - 13 May 2024
Abstract
Cyclists are considered to be vulnerable road users (VRUs) and need protection from potential collisions with cars and other vehicles induced by unsafe driving, dangerous road conditions, or weak cycling infrastructure. Integrating mmWave radars into cycling safety measures presents an efficient solution to [...] Read more.
Cyclists are considered to be vulnerable road users (VRUs) and need protection from potential collisions with cars and other vehicles induced by unsafe driving, dangerous road conditions, or weak cycling infrastructure. Integrating mmWave radars into cycling safety measures presents an efficient solution to this problem given their compact size, low power consumption, and low cost compared to other sensors. This paper introduces an mmWave radar-based bike safety system designed to offer real-time alerts to cyclists. The system consists of a low-power radar sensor affixed to the bicycle, connected to a micro-controller, and delivering a preliminary classification of detected obstacles. An efficient two-level clustering based on the accumulation of radar point clouds from multiple frames with a temporal projection from previous frames into the current frame is proposed. The clustering is followed by a coarse classification algorithm in which we use relevant features extracted from the resulting clusters. An annotated RadBike dataset composed of radar point cloud data synchronized with RGB camera images is developed to evaluate our system. The two-level clustering outperforms the DBSCAN algorithm, achieving a v-measure score of 0.91, compared to 0.88 with classical DBSCAN. Different classifiers, including decision trees, random forests, support vector machines (SVMs), and AdaBoost, have been assessed, with an overall accuracy of 87% for the three main object classes: four-wheeled, two-wheeled, and others. The system has the ability to improve rider safety on the road and substantially reduce the frequency of incidents involving cyclists. Full article
(This article belongs to the Section Radar Sensors)
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14 pages, 477 KiB  
Article
Growth, Hepatic Enzymatic Activity, and Quality of European Seabass Fed on Hermetia illucens and Poultry By-Product Meal in a Commercial Farm
by Lina Fernanda Pulido-Rodriguez, Leonardo Bruni, Giulia Secci, Sara Moutinho, Helena Peres, Tommaso Petochi, Giovanna Marino, Emilio Tibaldi and Giuliana Parisi
Animals 2024, 14(10), 1449; https://doi.org/10.3390/ani14101449 (registering DOI) - 13 May 2024
Abstract
Protein meals from insects in combination with poultry by-product meal appear to be promising ingredients for replacing conventional proteins in the diets of carnivorous fish. The present study explored the effects on growth performance, hepatic enzymatic activity, and fillet physical and nutritional characteristics [...] Read more.
Protein meals from insects in combination with poultry by-product meal appear to be promising ingredients for replacing conventional proteins in the diets of carnivorous fish. The present study explored the effects on growth performance, hepatic enzymatic activity, and fillet physical and nutritional characteristics during a 66-day feeding trial performed on European seabass. A total of 3000 fish were distributed into three tanks, where the control group was fed with a commercial diet (CG) and a second group was fed in duplicate with the experimental diet (SSH) containing 10% Hermetia illucens larva meal, 30% poultry by-product meal, and < 5.5 g/100 g of feed of marine origin proteins. All fish showed good growth performance. Glucose-6-phosphate dehydrogenase, aspartate aminotransferase, and 3-hydroxyacyl-CoA dehydrogenase activities were higher in the SSH group than in the CG group. The fillet fatty acid profile was largely unaffected by diet, except for a few fatty acids. Fish fed the SSH diet had a lower C22:1n-11 content than CG, thus suggesting an increased β-oxidation. The oxidative status of muscle lipids was not affected by the diet. In conclusion, the present study showed that European seabass can be successfully fed the SSH diet for two months in a commercial setting. Full article
3 pages, 242 KiB  
Editorial
Emerging Topics in Protein Crystallography
by Zhongzhou Chen and Giuseppe Zanotti
Int. J. Mol. Sci. 2024, 25(10), 5311; https://doi.org/10.3390/ijms25105311 (registering DOI) - 13 May 2024
Abstract
Protein crystallography is the discipline concerned with the determination of the three-dimensional structure of biological macromolecules in a crystalline state [...] Full article
(This article belongs to the Special Issue Emerging Topics in Protein Crystallography)
16 pages, 5490 KiB  
Article
The Next-Generation Probiotic E. coli 1917-pSK18a-MT Ameliorates Cadmium-Induced Liver Injury by Surface Display of Metallothionein and Modulation of Gut Microbiota
by Yan Zhang, Hong Huang, Chuanlin Luo, Xinfeng Zhang, Yanjing Chen, Fenfang Yue, Bingqing Xie, Tingtao Chen and Changwei Zou
Nutrients 2024, 16(10), 1468; https://doi.org/10.3390/nu16101468 (registering DOI) - 13 May 2024
Abstract
Cadmium (Cd) is recognized as being linked to several liver diseases. Currently, due to the limited spectrum of drugs available for the treatment of Cd intoxication, developing and designing antidotes with superior detoxification capacity and revealing their underlying mechanisms remains a major challenge. [...] Read more.
Cadmium (Cd) is recognized as being linked to several liver diseases. Currently, due to the limited spectrum of drugs available for the treatment of Cd intoxication, developing and designing antidotes with superior detoxification capacity and revealing their underlying mechanisms remains a major challenge. Therefore, we developed the first next-generation probiotic E. coli 1917-pSK18a-MT that delivers metallothionein (MT) to overcome Cd-induced liver injury in C57BL/6 mice by utilizing bacterial surface display technology. The results demonstrate that E. coli 1917-pSK18a-MT could efficiently express MT without altering the growth and probiotic properties of the strain. Moreover, we found that E. coli 1917-pSK18a-MT ameliorated Cd contamination-induced hepatic steatosis, inflammatory cell infiltration, and liver fibrosis by decreasing the expression of aminotransferases along with inflammatory factors. Activation of the Nrf2-Keap1 signaling pathway also further illustrated the hepatoprotective effects of the engineered bacteria. Finally, we showed that E. coli 1917-pSK18a-MT improved the colonic barrier function impaired by Cd induction and ameliorated intestinal flora dysbiosis in Cd-poisoned mice by increasing the relative abundance of the Verrucomicrobiota. These data revealed that the combination of E. coli 1917 and MT both alleviated Cd-induced liver injury to a greater extent and restored the integrity of colonic epithelial tissues and bacterial dysbiosis. Full article
(This article belongs to the Section Prebiotics and Probiotics)
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33 pages, 1231 KiB  
Article
Insights into Simulated Smart Mobility on Roundabouts: Achievements, Lessons Learned, and Steps Ahead
by Maria Luisa Tumminello, Elżbieta Macioszek and Anna Granà
Sustainability 2024, 16(10), 4079; https://doi.org/10.3390/su16104079 (registering DOI) - 13 May 2024
Abstract
This paper explores the domain of intelligent transportation systems, specifically focusing on roundabouts as potential solutions in the context of smart mobility. Roundabouts offer a safer and more efficient driving environment compared to other intersections, thanks to their curvilinear trajectories promoting speed control [...] Read more.
This paper explores the domain of intelligent transportation systems, specifically focusing on roundabouts as potential solutions in the context of smart mobility. Roundabouts offer a safer and more efficient driving environment compared to other intersections, thanks to their curvilinear trajectories promoting speed control and lower vehicular speeds for traffic calming. The synthesis review supported the authors in presenting current knowledge and emerging needs in roundabout design and evaluation. A focused examination of the models and methods used to assess safety and operational performance of roundabout systems was necessary. This is particularly relevant in light of new challenges posed by the automotive market and the influence of vehicle-to-vehicle communication on the conceptualization and design of this road infrastructure. Two case studies of roundabouts were analyzed in Aimsun to simulate the increasing market penetration rates of connected and autonomous vehicles (CAVs) and their traffic impacts. Through microscopic traffic simulation, the research evaluated safety and performance efficiency advancements in roundabouts. The paper concludes by outlining areas for further research and evolving perspectives on the role of roundabouts in the transition toward connected and autonomous vehicles and infrastructures. Full article
(This article belongs to the Special Issue Sustainable Road Transport System Planning and Optimization)
25 pages, 2538 KiB  
Review
Related Work and Motivation for Electric Vehicle Solar/Wind Charging Stations: A Review
by Radwan A. Almasri, Talal Alharbi, M. S. Alshitawi, Omar Alrumayh and Salman Ajib
World Electr. Veh. J. 2024, 15(5), 215; https://doi.org/10.3390/wevj15050215 (registering DOI) - 13 May 2024
Abstract
The shift towards sustainable transportation is an urgent worldwide issue, leading to the investigation of creative methods to decrease the environmental effects of traditional vehicles. Electric vehicles (EVs) are a promising alternative, but the issue lies in establishing efficient and environmentally friendly charging [...] Read more.
The shift towards sustainable transportation is an urgent worldwide issue, leading to the investigation of creative methods to decrease the environmental effects of traditional vehicles. Electric vehicles (EVs) are a promising alternative, but the issue lies in establishing efficient and environmentally friendly charging infrastructure. This review explores the existing research on the subject of photovoltaic-powered electric vehicle charging stations (EVCSs). Our analysis highlights the potential for economic growth and the creation of robust and decentralized energy systems by increasing the number of EVCSs. This review summarizes the current knowledge in this field and highlights the key factors driving efforts to expand the use of PV-powered EVCSs. The findings indicate that MATLAB was predominantly used for theoretical studies, with projects focusing on shading parking lots. The energy usage varied from 0.139 to 0.295 kWh/km, while the cost of energy ranged from USD 0.0032 to 0.5645 per kWh for an on-grid system. The payback period (PBP) values are suitable for this application. The average PBP was demonstrated to range from 1 to 15 years. The findings from this assessment can guide policymakers, researchers, and industry stakeholders in shaping future advancements toward a cleaner and more sustainable transportation system. Full article
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21 pages, 5513 KiB  
Article
Hydrological Factor and Land Use/Land Cover Change Explain the Vegetation Browning in the Dosso Reserve, Niger
by Yelong Zeng, Li Jia, Min Jiang, Chaolei Zheng, Massimo Menenti, Ali Bennour and Yunzhe Lv
Remote Sens. 2024, 16(10), 1728; https://doi.org/10.3390/rs16101728 (registering DOI) - 13 May 2024
Abstract
The West Sahel is facing significant threats to its vegetation and wildlife due to the land degradation and habitat fragmentation. It is crucial to assess the regional vegetation greenness dynamics in order to comprehensively evaluate the effectiveness of protection in the natural reserves. [...] Read more.
The West Sahel is facing significant threats to its vegetation and wildlife due to the land degradation and habitat fragmentation. It is crucial to assess the regional vegetation greenness dynamics in order to comprehensively evaluate the effectiveness of protection in the natural reserves. This study analyzes the vegetation greenness trends and the driving factors in the Dosso Partial Faunal Reserve in Niger and nearby unprotected regions—one of the most important habitats for endemic African fauna—using satellite time series data from 2001 to 2020. An overall vegetation browning trend was observed throughout the entire region with significant spatial variability. Vegetation browning dominated in the Dosso Reserve with 17.7% of the area showing a significant trend, while the area with significant greening was 6.8%. In a comparison, the nearby unprotected regions to the north and the east were found to be dominated by vegetation browning and greening, respectively. These results suggest that the vegetation protection practice was not fully effective throughout the Dosso Reserve. The dominant drivers were also diagnosed using the Random Forest model-based method and the Partial Dependence Plot tool, showing that water availability (expressed as soil moisture) and land use/land cover change were the most critical factors affecting vegetation greenness in the study region. Specifically, soil moisture stress and specific land management practices associated with logging, grazing, and land clearing appeared to dominate vegetation browning in the Dosso Reserve. In contrast, the vegetation greening in the central Dosso Reserve and the nearby unprotected region to the east was probably caused by the increase in shrubland/forest, which was related to the effective implementation of protection. These findings improve our understanding of how regional vegetation greenness dynamics respond to environmental changes in the Dosso Reserve and also highlight the need for more effective conservation planning and implementation to ensure sustainable socio-ecological development in the West Sahel. Full article
(This article belongs to the Special Issue Remote Sensing Applications in Monitoring of Protected Areas II)
11 pages, 4986 KiB  
Article
Gelatin-Coated TiO2/Pd Hybrid: A Potentially Useful Nanomaterial to Enhance Antibacterial and Anticancer Properties
by Periasamy Anbu, Muruganantham Rethinasabapathy, Anbazhagan Sathiyaseelan, Xin Zhang, Myeong-Hyeon Wang, Sekar Vijayakumar and Yun Suk Huh
Int. J. Mol. Sci. 2024, 25(10), 5308; https://doi.org/10.3390/ijms25105308 (registering DOI) - 13 May 2024
Abstract
Hybrid nanomaterials have attracted considerable interest in biomedicine because of their fascinating characteristics and wide range of applications in targeted drug delivery, antibacterial activity, and cancer treatment. This study developed a gelatin-coated Titanium oxide/palladium (TiO2/Pd) hybrid nanomaterial to enhance the antibacterial [...] Read more.
Hybrid nanomaterials have attracted considerable interest in biomedicine because of their fascinating characteristics and wide range of applications in targeted drug delivery, antibacterial activity, and cancer treatment. This study developed a gelatin-coated Titanium oxide/palladium (TiO2/Pd) hybrid nanomaterial to enhance the antibacterial and anticancer capabilities. Morphological and structural analyses were conducted to characterize the synthesized hybrid nanomaterial. The surface texture of the hybrid nanomaterials was examined by high-resolution transmission electron microscopy (HR-TEM) and field-emission scanning electron microscopy (FE-SEM). The FE-SEM image revealed the bulk of the spherically shaped particles and the aggregated tiny granules. Energy dispersive X-ray spectroscopy (EDS) revealed Ti, Pd, C, and O. X-ray diffraction (XRD) revealed the gelatin-coated TiO2/Pd to be in the anatase form. Fourier transform infrared spectroscopy examined the interactions among the gelatin-coated TiO2/Pd nanoparticles. The gelatin-coated TiO2/Pd nanomaterials exhibited high antibacterial activity against Escherichia coli (22 mm) and Bacillus subtilis (17 mm) compared to individual nanoparticles, confirming the synergistic effect. More importantly, the gelatin-coated TiO2/Pd hybrid nanomaterial exhibited remarkable cytotoxic effects on A549 lung cancer cells which shows a linear increase with the concentration of the nanomaterial. The hybrid nanomaterials displayed higher toxicity to cancer cells than the nanoparticles alone. Furthermore, the cytotoxic activity against human cancer cells was verified by the generation of reactive oxygen species and nuclear damage. Therefore, gelatin-coated TiO2/Pd nanomaterials have potential uses in treating cancer and bacterial infections. Full article
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26 pages, 2196 KiB  
Article
Study on the Typical Environmental Factors in the Middle Part of Zhoushan Fishery Based on HY-1C/D and Other Multi-Source Data
by Xinkai Zhang, Lina Cai, Menghan Yu and Rong Tang
Water 2024, 16(10), 1387; https://doi.org/10.3390/w16101387 (registering DOI) - 13 May 2024
Abstract
This study utilizes satellite data, including HY-1C/D, along with reanalysis data, to unveil the typical environmental characteristics of the sea surface in the middle of Zhoushan fishery. The article addresses three main issues. The first one is the development of an ocean primary [...] Read more.
This study utilizes satellite data, including HY-1C/D, along with reanalysis data, to unveil the typical environmental characteristics of the sea surface in the middle of Zhoushan fishery. The article addresses three main issues. The first one is the development of an ocean primary productivity (OPP) inversion algorithm model. The second one is the study of chlorophyll-α (Chl-α) concentration and OPP distribution characteristics in Zhoushan fishery using China’s domestically produced ocean satellite HY-1C/D CZI data. The last one is the revelation of the characteristics of typical environmental factors on the sea surface at Zhoushan fishery by combining HY-1C/D with multi-source data. The results show the following: (1) The middle part of Zhoushan fishery exhibits significant seasonal and regional variations in Chl-α concentration and OPP. Chl-α concentration ranges mainly between 0.2 and 2.9 µg/L, with higher concentrations in spring and summer and lower Chl-α concentrations in autumn and winter. Spatially, Chl-α concentration gradually decreases from west to east. The OPP in the study area ranges from 100 mg·m−2d−1 to 1000 mg·m−2d−1, with high OPP values distributed on the western side, ranging from 400 mg·m−2d−1 to 1000 mg·m−2d−1, and gradually decreasing seaward. The highest OPP occurs in summer and the lowest in winter. (2) The correlation analysis between Chl-α concentration and OPP revealed a strong positive relationship. Consequently, this study developed an empirical model for estimating OPP based on Chl-α concentration and validated its feasibility. The model applies to areas with Chl-α concentrations ranging from 0.2 to 4 µg/L. (3) The convergence of freshwater injection, multiple ocean currents, and seasonal upwelling in the study area brings about a rich supply of nutrients. Additionally, the region is characterized by suitable conditions, including optimal Chl-α concentrations, OPP, SST, salinity, currents, and geological water depths. The synergistic effect of these factors together contributed to the formation of Zhoushan fishery. Full article
(This article belongs to the Section Oceans and Coastal Zones)
21 pages, 2766 KiB  
Article
Identification of Important Wetlands and Optimization of Landscape Patterns Based on Human Pressure Index: The Case of the Linghekou Wetland in China
by Meiqing Wang, Qian Cheng and Ruixin Chen
Sustainability 2024, 16(10), 4082; https://doi.org/10.3390/su16104082 (registering DOI) - 13 May 2024
Abstract
The Linghekou wetland is a rich repository of ecological resources and serves as an important habitat for numerous rare and protected animals. However, due to a confluence of natural and anthropogenic factors, the ecological environment of the Linghekou wetland is facing a multitude [...] Read more.
The Linghekou wetland is a rich repository of ecological resources and serves as an important habitat for numerous rare and protected animals. However, due to a confluence of natural and anthropogenic factors, the ecological environment of the Linghekou wetland is facing a multitude of threats, including the reduction in wetland area, the degradation of wetland resources, and the instability of ecological structure. This paper employs an anthropogenic focus, utilizing the human pressure index (HPI), spatial autocorrelation, and cold and hot spot methods to identify crucial wetlands. These identified wetlands are then utilized as ecological source sites to optimize the landscape pattern of the Linghekou wetland, employing the minimum cumulative resistance (MCR) model. The final results indicated the identification of 6 ecological sources, 8 ecological corridors, and 42 ecological nodes. These were primarily concentrated in the southern region of the study area and were distributed in a reasonable manner. The method of identifying ecological sources when optimizing the landscape pattern with the MCR model was enriched by this approach. Additionally, the paper offers recommendations for the optimization of the landscape pattern of the Linghekou wetland and establishes a foundation for the protection and restoration of other similar wetlands. Full article
11 pages, 255 KiB  
Article
Predictive Factors for Major Complications and Urological Cancer Diagnosis in Older Adults (≥80 Years) Admitted to the Emergency Department for Hematuria
by Mauro Ragonese, Daniele Fettucciari, Luigi Carbone, Filippo Gavi, Marco Montesi, Eros Scarciglia, Pierluigi Russo, Domenico Maria Sanesi, Filippo Marino, Nazario Foschi, Francesco Pinto, Francesco Franceschi, Marco Racioppi, Emilio Sacco and Marcello Covino
J. Clin. Med. 2024, 13(10), 2874; https://doi.org/10.3390/jcm13102874 (registering DOI) - 13 May 2024
Abstract
Background: Gross Hematuria is a relevant cause of admission to the emergency department in the general population and particularly in older adults (≥80 years). This specific urological symptom is often underestimated and usually associated with benign conditions such as urinary infections or [...] Read more.
Background: Gross Hematuria is a relevant cause of admission to the emergency department in the general population and particularly in older adults (≥80 years). This specific urological symptom is often underestimated and usually associated with benign conditions such as urinary infections or poor hydration. Nevertheless, hematuria could lead to severe acute complications or be the first symptom of urological cancers. Methods: We retrospectively analyzed clinical data from 1169 patients aged ≥80 years consecutively admitted to the emergency department for hematuria. The primary endpoint of the study was to identify risk factors for major complications, and the secondary endpoint was to analyze risk factors for urological cancer diagnosis. The median age was 85 years (IQR 82–88 years), and 908 (77%) were males. Among them, 449 (38.4%) had a past medical history of urological neoplasm (kidney, ureter, bladder, prostate, or urethral cancer). Results: Overall, 87 patients (7.4%) had major complications (patient death, septic shock, and admission to the intensive care unit). Worse vital signs at admission, fever, and confusion (p < 0.001, OR 18.0 IC 95% [5.5–58.7]; p = 0.015, OR 2.0 IC 95% [1.1–3.5]; p = <0.001, OR 4.2 IC 95% [1.9–3.5], respectively), as well as lower hemoglobin values and higher Charlson comorbidity index (p < 0.001, OR 0.8 IC 95% [0.7–0.9]), p = 0.002, OR = 1.2 [1.1–1.3]) were independent predictive factors for major complications. The multivariate analysis identified as risk factors for diagnosis of urological cancer older age, male sex and higher comorbidity (OR 1.05 IC95% [1–1.09]; OR 2.19 IC95% [1.42–3.39] and OR 1.11 IC95% [1.2–1.2], respectively); interestingly the presence of indwelling vesical catheter (IVC) (OR 0.44 IC95% [0.24–0.82]) resulted as an independent factor for absence of urological cancers. Conclusions: Hematuria is a frequent symptom in older adults admitted to the emergency department. While this is often associated with benign conditions, there are some risk factors for major complications and for urological cancer that must be taken into account to identify the patients who need further evaluation or prompt hospital admission. Full article
(This article belongs to the Special Issue Clinical Advances in Urologic Oncology)
30 pages, 3558 KiB  
Article
Multi-Strategy Improved Dung Beetle Optimization Algorithm and Its Applications
by Mingjun Ye , Heng Zhou, Haoyu Yang, Bin Hu and Xiong Wang
Biomimetics 2024, 9(5), 291; https://doi.org/10.3390/biomimetics9050291 (registering DOI) - 13 May 2024
Abstract
The dung beetle optimization (DBO) algorithm, a swarm intelligence-based metaheuristic, is renowned for its robust optimization capability and fast convergence speed. However, it also suffers from low population diversity, susceptibility to local optima solutions, and unsatisfactory convergence speed when facing complex optimization problems. [...] Read more.
The dung beetle optimization (DBO) algorithm, a swarm intelligence-based metaheuristic, is renowned for its robust optimization capability and fast convergence speed. However, it also suffers from low population diversity, susceptibility to local optima solutions, and unsatisfactory convergence speed when facing complex optimization problems. In response, this paper proposes the multi-strategy improved dung beetle optimization algorithm (MDBO). The core improvements include using Latin hypercube sampling for better population initialization and the introduction of a novel differential variation strategy, termed “Mean Differential Variation”, to enhance the algorithm’s ability to evade local optima. Moreover, a strategy combining lens imaging reverse learning and dimension-by-dimension optimization was proposed and applied to the current optimal solution. Through comprehensive performance testing on standard benchmark functions from CEC2017 and CEC2020, MDBO demonstrates superior performance in terms of optimization accuracy, stability, and convergence speed compared with other classical metaheuristic optimization algorithms. Additionally, the efficacy of MDBO in addressing complex real-world engineering problems is validated through three representative engineering application scenarios namely extension/compression spring design problems, reducer design problems, and welded beam design problems. Full article
(This article belongs to the Special Issue Nature-Inspired Metaheuristic Optimization Algorithms 2024)
15 pages, 655 KiB  
Article
Exploring Aggressive Behaviors in Greek Secondary Schools: Prevalence, Sociodemographic Factors, and Comparative Analysis with Elementary School Students
by Argyro Bourou, Petros Karkalousos, Anastasios G. Kriebardis and Effie Papageorgiou
Behav. Sci. 2024, 14(5), 405; https://doi.org/10.3390/bs14050405 (registering DOI) - 13 May 2024
Abstract
The main objectives of this study are to determine the prevalence of bullying in Greek secondary schools and detect the possible characteristics of bullies’ profiles in Greek school settings. A structured questionnaire was given to one hundred ninety-two (n= 192) educators at Greek [...] Read more.
The main objectives of this study are to determine the prevalence of bullying in Greek secondary schools and detect the possible characteristics of bullies’ profiles in Greek school settings. A structured questionnaire was given to one hundred ninety-two (n= 192) educators at Greek junior high schools in urban and rural areas. The educators were asked to report the frequencies and forms of aggressive behavior observed during the 2022–2023 school year, the bullies’ sociodemographic characteristics, and ways of dealing with bullying episodes. The data are presented, after conducting statistical analyses, in comparison with data for elementary school students. The results revealed that higher rates of bullying were reported compared with elementary school children. Moreover, according to teachers’ observations, aggressive behavior is independent of a pupil’s diagnosis, but specific types are correlated significantly with a pupil’s gender, nationality, low academic performance, and popularity. Factor analysis showed two main factors of aggression types, where common points and differences with elementary school students are mentioned. Implementations for the prevention of school bullying are discussed. Full article
13 pages, 2299 KiB  
Article
Effects of the Dual FAAH/MAGL Inhibitor AKU-005 on Trigeminal Hyperalgesia in Male Rats
by Rosaria Greco, Chiara Demartini, Miriam Francavilla, Anna Maria Zanaboni, Sara Facchetti, Michela Palmisani, Valentina Franco and Cristina Tassorelli
Cells 2024, 13(10), 830; https://doi.org/10.3390/cells13100830 (registering DOI) - 13 May 2024
Abstract
The inhibition of endocannabinoid hydrolysis by enzymatic inhibitors may interfere with mechanisms underlying migraine-related pain. The dual FAAH/MAGL inhibitor AKU-005 shows potent inhibitory activity in vitro. Here, we assessed the effect of AKU-005 in a migraine animal model based on nitroglycerin (NTG) administration. [...] Read more.
The inhibition of endocannabinoid hydrolysis by enzymatic inhibitors may interfere with mechanisms underlying migraine-related pain. The dual FAAH/MAGL inhibitor AKU-005 shows potent inhibitory activity in vitro. Here, we assessed the effect of AKU-005 in a migraine animal model based on nitroglycerin (NTG) administration. Male rats were treated with AKU-005 (0.5 mg/kg, i.p.) or vehicle 3 h after receiving NTG (10 mg/kg, i.p.) or NTG vehicle. One hour later, rats were subjected to the open field test followed by the orofacial formalin test. At the end of the test, we collected serum samples for assessing calcitonin gene-related peptide (CGRP) levels as well as meninges, trigeminal ganglia, and brain areas to assess mRNA levels of CGRP and pro-inflammatory cytokines, and endocannabinoid and related lipid levels. AKU-005 reduced NTG-induced hyperalgesia during the orofacial formalin test but did not influence NTG-induced changes in the open field test. It significantly reduced serum levels of CGRP, CGRP, and pro-inflammatory cytokine mRNA levels in the meninges, trigeminal ganglia, and central areas. Surprisingly, AKU-005 caused no change in endocannabinoids and related lipids in the regions evaluated. The present findings suggest that AKU-005 may have anti-migraine effects by reducing CGRP synthesis and release and the associated inflammatory events. This effect, however, does not seem mediated via an interference with the endocannabinoid pathway. Full article
(This article belongs to the Special Issue Migraine Neuroscience: From Experimental Models to Target Therapy)
12 pages, 321 KiB  
Article
Ecofeminism and the Cultural Affinity to Genocidal Capitalism: Theorising Necropolitical Femicide in Contemporary Greece
by Anastasia Christou
Soc. Sci. 2024, 13(5), 263; https://doi.org/10.3390/socsci13050263 (registering DOI) - 13 May 2024
Abstract
Resilient necrocapitalism and the zombie genre of representations of current dystopias are persistent in their political purpose in producing changes in the social order to benefit plutocracies around the world. It is through a thanatopolitical lens that we should view the successive losses [...] Read more.
Resilient necrocapitalism and the zombie genre of representations of current dystopias are persistent in their political purpose in producing changes in the social order to benefit plutocracies around the world. It is through a thanatopolitical lens that we should view the successive losses of life, and this zombie genre has come to represent a dystopia that, for political purposes, is intended to produce changes in societies which have tolerated the violent deaths of women. This article focuses on contemporary Greece and proposes a theoretical framework where femicide is understood as a social phenomenon that reflects a global gendered necropolitical logic which equals genocide. Such theoretical assemblages have to be situated within intersectional imperatives and tacitly as the result of the capitalist terror state performed in an expansive and direct immediate death, exacerbated by the lingering slow social death of the welfare state. The article contends that the scripted hetero-patriarchal social order of the necrocapitalist state poses a unique political threat to societies. With the silence of the complicity of the state, what is necessary is the creation and spread of new political knowledge and new social movements as resilient political tactics of resistance. This article foregrounds an ecofeminist perspective on these issues and considers ways through which new pedagogies of hope can counter the gendered necropolitics of contemporary capitalism in Greece. Full article
(This article belongs to the Special Issue Feminist Solidarity, Resistance, and Social Justice)
20 pages, 719 KiB  
Article
Application and Challenges of the Technology Acceptance Model in Elderly Healthcare: Insights from ChatGPT
by Sang Dol Kim
Technologies 2024, 12(5), 68; https://doi.org/10.3390/technologies12050068 (registering DOI) - 13 May 2024
Abstract
The Technology Acceptance Model (TAM) plays a pivotal role in elderly healthcare, serving as a theoretical framework. This study aimed to identify TAM’s core components, practical applications, challenges arising from its applications, and propose countermeasures in elderly healthcare. This descriptive study was conducted [...] Read more.
The Technology Acceptance Model (TAM) plays a pivotal role in elderly healthcare, serving as a theoretical framework. This study aimed to identify TAM’s core components, practical applications, challenges arising from its applications, and propose countermeasures in elderly healthcare. This descriptive study was conducted by utilizing OpenAI’s ChatGPT, with an access date of 10 January 2024. The three open-ended questions administered to ChatGPT and its responses were collected and qualitatively evaluated for reliability through previous studies. The core components of TAMs were identified as perceived usefulness, perceived ease of use, attitude toward use, behavioral intention to use, subjective norms, image, and facilitating conditions. TAM’s application areas span various technologies in elderly healthcare, such as telehealth, wearable devices, mobile health apps, and more. Challenges arising from TAM applications include technological literacy barriers, digital divide concerns, privacy and security apprehensions, resistance to change, limited awareness and information, health conditions and cognitive impairment, trust and reliability concerns, a lack of tailored interventions, overcoming age stereotypes, and integration with traditional healthcare. In conclusion, customized interventions are crucial for successful tech acceptance among the elderly population. The findings of this study are expected to enhance understanding of elderly healthcare and technology adoption, with insights gained through natural language processing models like ChatGPT anticipated to provide a fresh perspective. Full article
15 pages, 2170 KiB  
Article
Relative Expression of Genes Elicited by Clonostachys rosea in Pinus radiata Induces Systemic Resistance
by Priscila Moraga-Suazo, Regis Le-Feuvre, Dario Navarrete and Eugenio Sanfuentes
Forests 2024, 15(5), 854; https://doi.org/10.3390/f15050854 (registering DOI) - 13 May 2024
Abstract
Radiata pine is one of the most commonly planted tree species in Chile due to its fast growth and desirable wood and pulp properties. However, its productivity is hampered by several diseases. Pitch canker disease (PCC) caused by Fusarium circinatum, is considered [...] Read more.
Radiata pine is one of the most commonly planted tree species in Chile due to its fast growth and desirable wood and pulp properties. However, its productivity is hampered by several diseases. Pitch canker disease (PCC) caused by Fusarium circinatum, is considered the most damaging disease to the pine forest industry. Several control measures have been established, with biological control emerging as an environmentally friendly and effective way for F. circinatum control. Previous studies support the value of Clonostachys rosea in reducing PCC damage, with evidence suggesting a potential induced systemic resistance (ISR) triggered in radiata pines by this agent. Ten-month-old radiata pine plants were pre-treated with C. rosea on a substrate at 8 and 1 days before inoculation with F. circinatum on the stem tip, and expression levels were determined for DXS1, LOX, PAL, and PR3 genes 24 h later. Lesion length was 45% lower on plants pre-treated with C. rosea and infected with F. circinatum compared to non-pre-treated and infected plants. Additionally, LOX and PR3 were induced 23 and 62 times more, respectively, in comparison to untreated plants. Our results indicate that C. rosea causes an ISR response in pre-treated plants, significantly increasing the expression of resistance genes and reducing lesion length. Full article
(This article belongs to the Section Forest Health)
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21 pages, 8970 KiB  
Article
Lactobacillus delbrueckii subsp. bulgaricus Alleviates Acute Injury in Hypoxic Mice
by Ke Song, Hui Ling, Linlin Wang, Peijun Tian, Xing Jin, Jianxin Zhao, Wei Chen, Gang Wang and Yujing Bi
Nutrients 2024, 16(10), 1465; https://doi.org/10.3390/nu16101465 (registering DOI) - 13 May 2024
Abstract
Acute mountain sickness (AMS) is a common ailment in high-altitude areas caused by the body’s inadequate adaptation to low-pressure, low-oxygen environments, leading to organ edema, oxidative stress, and impaired intestinal barrier function. The gastrointestinal tract, being the first to be affected by ischemia [...] Read more.
Acute mountain sickness (AMS) is a common ailment in high-altitude areas caused by the body’s inadequate adaptation to low-pressure, low-oxygen environments, leading to organ edema, oxidative stress, and impaired intestinal barrier function. The gastrointestinal tract, being the first to be affected by ischemia and hypoxia, is highly susceptible to injury. This study investigates the role of Lactobacillus delbrueckii subsp. bulgaricus in alleviating acute hypoxic-induced intestinal and tissue damage from the perspective of daily consumed lactic acid bacteria. An acute hypoxia mouse model was established to evaluate tissue injury, oxidative stress, inflammatory responses, and intestinal barrier function in various groups of mice. The results indicate that strain 4L3 significantly mitigated brain and lung edema caused by hypoxia, improved colonic tissue damage, and effectively increased the content of tight junction proteins in the ileum, reducing ileal permeability and alleviating mechanical barrier damage in the intestines due to acute hypoxia. Additionally, 4L3 helped to rebalance the intestinal microbiota. In summary, this study found that Lactobacillus delbrueckii subsp. bulgaricus strain 4L3 could alleviate acute intestinal damage caused by hypoxia, thereby reducing hypoxic stress. This suggests that probiotic lactic acid bacteria that exert beneficial effects in the intestines may alleviate acute injury under hypoxic conditions in mice, offering new insights for the prevention and treatment of AMS. Full article
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20 pages, 4796 KiB  
Article
ABNet: An Aggregated Backbone Network Architecture for Fine Landcover Classification
by Bo Si, Zhennan Wang, Zhoulu Yu and Ke Wang
Remote Sens. 2024, 16(10), 1725; https://doi.org/10.3390/rs16101725 (registering DOI) - 13 May 2024
Abstract
High-precision landcover classification is a fundamental prerequisite for resource and environmental monitoring and land-use status surveys. Imbued with intricate spatial information and texture features, very high spatial resolution remote sensing images accentuate the divergence between features within the same category, thereby amplifying the [...] Read more.
High-precision landcover classification is a fundamental prerequisite for resource and environmental monitoring and land-use status surveys. Imbued with intricate spatial information and texture features, very high spatial resolution remote sensing images accentuate the divergence between features within the same category, thereby amplifying the complexity of landcover classification. Consequently, semantic segmentation models leveraging deep backbone networks have emerged as stalwarts in landcover classification tasks owing to their adeptness in feature representation. However, the classification efficacy of a solitary backbone network model fluctuates across diverse scenarios and datasets, posing a persistent challenge in the construction or selection of an appropriate backbone network for distinct classification tasks. To elevate the classification performance and bolster the generalization of semantic segmentation models, we propose a novel semantic segmentation network architecture, named the aggregated backbone network (ABNet), for the meticulous landcover classification. ABNet aggregates three prevailing backbone networks (ResNet, HRNet, and VoVNet), distinguished by significant structural disparities, using a same-stage fusion approach. Subsequently, it amalgamates these networks with the Deeplabv3+ head after integrating the convolutional block attention mechanism (CBAM). Notably, this amalgamation harmonizes distinct scale features extracted by the three backbone networks, thus enriching the model’s spatial contextual comprehension and expanding its receptive field, thereby facilitating more effective semantic feature extraction across different stages. The convolutional block attention mechanism primarily orchestrates channel adjustments and curtails redundant information within the aggregated feature layers. Ablation experiments demonstrate an enhancement of no less than 3% in the mean intersection over union (mIoU) of ABNet on both the LoveDA and GID15 datasets when compared with a single backbone network model. Furthermore, in contrast to seven classical or state-of-the-art models (UNet, FPN, PSPNet, DANet, CBNet, CCNet, and UPerNet), ABNet evinces excellent segmentation performance across the aforementioned datasets, underscoring the efficiency and robust generalization capabilities of the proposed approach. Full article
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21 pages, 4542 KiB  
Article
Experimental Study on the Clogging Performance of Waste Slag
by Shibo Li, Jinduo Chen, Jianquan Ma, Hao Li, Hao Guo, Yongqiang Qiu, Fuli Han and Yashu Ji
Water 2024, 16(10), 1390; https://doi.org/10.3390/w16101390 (registering DOI) - 13 May 2024
Abstract
The fine particles around a mining area are easy to transport under the climatic and hydrological actions such as rainfall, that causes the change in the permeability of accumulated slag and increases the hazard probability of slag debris flow. In this study, eight [...] Read more.
The fine particles around a mining area are easy to transport under the climatic and hydrological actions such as rainfall, that causes the change in the permeability of accumulated slag and increases the hazard probability of slag debris flow. In this study, eight experiments were designed to discuss the influence of fine particle migration on the permeability characteristics and clogging of slag accumulation in different graded particles and different dry densities. The results of experiments with coarse slags of five different particle sizes show that the ratio ranging from four to six in the coarse slag size and fine size caused a significant clogging phenomenon. It is confirmed that the shape of the particles is one of the factors affecting the clogging of coarse soil besides the coarse and fine particle size, and the clogging assessment criterion for slag and the corresponding clogging patterns based on the slag shape characteristics are given. And through three kinds of different dry density-graded slag, three clogging experiments were completed to verify the clogging standard and clogging particle size. The experimental results show that the clogging particle size obtained by the clogging criteria can effectively reduce the permeability of slag accumulation, and it is considered that the equivalent particle size and particle shape characteristics are the main factors affecting the clogging performance of accumulation, while the dry density of deposits has no significant influence on it. Full article
(This article belongs to the Topic Landslides and Natural Resources)

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