Mosses host diverse bacterial communities essential for their fitness, nutrient acquisition, stress tolerance, and pathogen defense. Understanding the microbiome’s taxonomic composition is the first step, but unraveling their functional capabilities is crucial for grasping their ecological significance. Metagenomics characterizes microbial communities by composition, while metatranscriptomics explores gene expression, providing insights into microbiome functionality beyond the structure. Here, we present for the first time a metatranscriptomic study of two moss species, Hypnum cupressiforme (Hedw.) and Platyhypnidium riparioides (Hedw.) Dixon., renowned as key biomonitors of atmospheric and water pollution. Our investigation extends beyond taxonomic profiling and offers a profound exploration of moss bacterial communities. Pseudomonadota and Actinobacteria are the dominant bacterial phyla in both moss species, but their proportions differ. In H. cupressiforme, Actinobacteria make up 62.45% and Pseudomonadota 32.48%, while in P. riparioides, Actinobacteria account for only 25.67% and Pseudomonadota 69.08%. This phylum-level contrast is reflected in genus-level differences. Our study also shows the expression of most genes related to nitrogen cycling across both microbiomes. Additionally, functional annotation highlights disparities in pathway prevalence, including carbon dioxide fixation, photosynthesis, and fatty acid biosynthesis, among others. These findings hint at potential metabolic distinctions between microbial communities associated with different moss species, influenced by their specific genotypes and habitats. The integration of metatranscriptomic data holds promise for enhancing our understanding of bryophyte–microbe partnerships, opening avenues for novel applications in conservation, bioremediation, and sustainable agriculture. Full article

The main aim of this article is to analyse the Si solar cell’s behaviour when it is exposed to light of different colours. An experimental work, with 100 experimental tests, was carried out, using an RGB LED. In order to obtain better fitting of the characteristic curves’ results, we used a novel discrete model, d1MxP. The obtained results showed that all experimental points of the tests were inside the two triangles that connected the three theoretical primary colours and the three experimental primary colours in the chromaticity diagram. With this diagram, the colour purity could be determined, which presented values between 20% and 60%. The primary colours of the three different LEDs of the light source presented a dominant wavelength that corresponded to the peak wavelengths of the light source spectrum, which showed high purity. However, the obtained results show that mixing colours may not lead to an increase in the cell’s output power. Additionally, an increase in the cell’s temperature was observed, due to the surplus absorbed energy, which was converted into heat, being one of the causes of the cell’s efficiency reduction. Full article

The present study investigated whether different grafting combinations (hypobiont-epibiont) of Vitis spp. influence root infection with phylloxera and thus vine biomass on potted 2-year-old plants. The study was conducted simultaneously at two locations in Slovenia (VEM) and Hungary (GF). The dormant canes of ‘Johanniter’ (JOH), ‘Riesling’ (RR) and Vitis berlandieri × Vitis riparia ‘Teleki 5C’ (5C) were hetero-grafted (each with each) and autografted (each with itself), so that nine plant combinations were used for the trial. The roots of the experimental plants at different ages (1–2 years) were infested with two phylloxera populations originating from two locations (VEM, GF). Plant growth was quantified 120 days after inoculation by measuring root and shoot biomass, while the extent of phylloxera infestation was assessed by the number of feeding sites (nodosities, tuberosities) and the number of larval stages of phylloxera. In most cases, the genotype of the hypobiont influenced the degree of phylloxera infestation on all roots of the two-year-old root system. At both locations, the highest number of nodosities and the highest increase in phylloxera population was observed on the autografted Teleki 5C (5C/5C). The phylloxera biotype derived from Vitis vinifera roots (GF) induced tuberosities, especially on roots of combinations where JOH and RR were used as hypobionts. No correlation was found between biomass and phylloxera infestation. The hypobiont genotype had no effect on cane biomass at the end of the growth cycle at either experimental site. Full article

Single-phase center multiple azimuth beam (SPC MAB) mode is an effective method for high-resolution wide-swath (HRWS) SAR imaging. The traditional azimuth spectrum reconstruction method for SPC MAB mode is based on the combination scheme from which fake targets along the azimuth direction arise because the inter-beam interference is not considered. When the real antenna mode is inconsistent with the ideal one, the disadvantages of the combination scheme become more serious. In this paper, based on the basic theory of the low-pass, band-limited, multiple-channel under-sampling and reconstruction, a novel digital beam-forming method is proposed for the SPC MAB imaging mode with ideal antenna mode first. The method analyzes the system functions of the sub-beams, based on which digital beam-forming filters are designed for all the sub-beams. The designed filters can reconstruct the correct wide-bandwidth azimuth spectrum and suppress the inter-beam interference simultaneously. Furthermore, the proposed method is extended to SPC MAB mode with the non-ideal antenna mode. The simulation experiments prove the validities of the proposed method both for azimuth spectral reconstruction and the inter-beams interfering suppressing, no matter that the SPC MAB’s antenna mode is ideal or non-ideal. Full article

Oil–paper insulation is the critical insulation element in the modern power system. Under a harsh operating environment, oil–paper insulation will deteriorate gradually, resulting in electrical accidents. Thus, it is important to evaluate and monitor the insulation state of oil–paper insulation. Firstly, this paper introduces the geometric structure and physical components of oil–paper insulation and shows the main reasons and forms of oil–paper insulation’s degradation. Then, this paper reviews the existing condition assessment techniques for oil–paper insulation, such as the dissolved gas ratio analysis, aging kinetic model, cellulose–water adsorption isotherm, oil–paper moisture balance curve, and dielectric response technique. Additionally, the advantages and limitations of the above condition assessment techniques are discussed. In particular, this paper highlights the dielectric response technique and introduces its evaluation principle in detail: (1) collecting the dielectric response data, (2) extracting the feature parameters from the collected dielectric response data, and (3) establishing the condition assessment models based on the extracted feature parameters and the machine learning techniques. Finally, two full potential studies are proposed, which research hotspots’ oil–paper insulation and the electrical–chemical joint evaluation technique. In summary, this paper concludes the principles, advantages and limitation of the existing condition assessment techniques for oil–paper insulation, and we put forward two potential research avenues. Full article

HHLA2 is a checkpoint from the B7 family that can play a co-stimulatory or co-inhibitory role in cancer, depending on the binding receptor. The aim of this meta-analysis was to assess the relationship between HHLA2 levels and its impact on the prognosis of patients with solid cancers. The study used data from PubMed, Embase, Web of Science (WOS), Cochrane and SCOPUS databases. The R studio software was used for the data analysis. The study assessed overall survival (OS), disease-specific survival (DSS), progression-free survival (PFS), recurrence-free survival (RFS), and disease-free survival (DFS) by pooling appropriate hazard ratios (HR). Eighteen studies (2880 patients’ data) were included. High expression of HHLA2 was associated with worse OS (HR = 1.58, 95% CI: 1.23–2.03), shorter RFS (HR = 1.95, 95% CI: 1.38–2.77) and worse DFS (HR = 1.45, 95% CI: 1.01–2.09) in patients with solid cancers. The current study suggests that high expression of HHLA2 is associated with poorer prognosis in patients with solid cancers. Full article

The ongoing challenge to ensure a sustainable and affordable energy supply forces industrial companies to transform their energy system. This transformation usually leads to an increase in topological complexity, which in turn results in increased operational complexity. Existing approaches from the field of supervisory and optimal control are capable of mastering complex operational problems. However, due to the complex and non-transparent implementation, there is still no industrial penetration, which hinders the necessary transformation of energy systems. This work aims at establishing trust in these control approaches and presents a procedure model for the systematic development of application-oriented operating strategies for industrial energy heating systems. It combines research approaches from the fields of sequencing control and approximate MPC to extract rule-based operating strategies, which are inherently easy to understand and implementable. By splitting the procedure model into five phases, expert knowledge can be integrated in a targeted manner. The procedure model is validated by the exemplary application to an industrial heating supply system. As part of an optimization study, the operating strategy developed is compared with both an MPC strategy and a baseline strategy. While the conventional MPC approach represents the upper limit of optimality, the operating strategy developed is able to achieve comparable results. Compared to the baseline strategy, a relative reduction in operating costs of 5.4% to 37.0% is achieved. Full article

UDP-glycosyltransferases (UGTs) are a diverse superfamily of enzymes. Insects utilize uridine diphosphate-glucose (UDP-glucose) as a glycosyl donor for glycosylation in vivo, involved in the glycosylation of lipophilic endosymbionts and xenobiotics, including phytotoxins. UGTs act as second-stage detoxification metabolizing enzymes, which are essential for the detoxification metabolism of insecticides and benzoxazine compounds. However, the UGT genes responsible for specific glycosylation functions in S. frugiperda are unclear at present. In this study, we utilized CRISPR/Cas9 to produce a SfUGT50A15-KO strain to explore its possible function in governing sensitivity to chemical insecticides or benzoxazinoids. The bioassay results suggested that the SfUGT50A15-KO strain was significantly more sensitive to chlorantraniliprole, emamectin benzoate, and benzoxazinoids than the wild-type strains. This finding suggests that the overexpression of the SfUGT50A15 gene may be linked to S. frugiperda resistance to pesticides (chlorantraniliprole and emamectin benzoate) as well as benzoxazinoids (BXDs). Full article

The Sturm–Liouville boundary value problem (SLBVP) stands as a fundamental cornerstone in the realm of mathematical analysis and physical modeling. Also known as the Sturm–Liouville problem (SLP), this paper explores the intricacies of this classical problem, particularly the relationship between its canonical and Liouville normal (Schrödinger) forms. While the conversion from the canonical to Schrödinger form using Liouville’s transformation is well known in the literature, the inverse transformation seems to be neglected. Our study attempts to fill this gap by investigating the inverse of Liouville’s transformation, that is, given any SLP in the Schrödinger form with some invariant function, we seek the SLP in its canonical form. By closely examining the second Paine–de Hoog–Anderson (PdHA) problem, we argue that retrieving the SLP in its canonical form can be notoriously difficult and can even be impossible to achieve in its exact form. Finding the inverse relationship between the two independent variables seems to be the main obstacle. We confirm this claim by considering four different scenarios, depending on the potential and density functions that appear in the corresponding invariant function. In the second PdHA problem, this invariant function takes a reciprocal quadratic binomial form. In some cases, the inverse Liouville transformation produces an exact expression for the SLP in its canonical form. In other situations, however, while an exact canonical form is not possible to obtain, we successfully derived the SLP in its canonical form asymptotically. Full article

Introduction: Thyroid nodule incidence is increasing due to the widespread application of ultrasonography. Fine-needle aspiration cytology is widely applied for the detection of malignancies. The aim of this study was to evaluate the predictive value of ultrasonography in thyroid cancer. Methods: This retrospective study included patients that underwent total thyroidectomy for benign thyroid disease or well-differentiated thyroid carcinoma from January 2017 to December 2022. The study population was divided into groups: the well-differentiated thyroid cancer group and the control group with benign histopathological reports. Results: In total, 192 patients were enrolled in our study; 159 patients were included in the well-differentiated thyroid cancer group and 33 patients in the control group. Statistical analysis demonstrated that ultrasonographic findings such as microcalcifications (90.4%), hypoechogenicity (89.3%), irregular margins (92.2%) and taller-than-wide shape (90.5%) were correlated to malignancy (p < 0.001). Uni- and multivariate analysis revealed that both US score (OR: 2.177; p < 0.001) and Bethesda System (OR: 1.875; p = 0.002) could predict malignancies. In terms of diagnostic accuracy, the US score displayed higher sensitivity (64.2% vs. 33.3%) and better negative predictive value (34.5% vs. 24.4%) than the Bethesda score, while both scoring systems displayed comparable specificities (90.9% vs. 100%) and positive predictive values (97.1% vs. 100%). Discussion: The malignant potential of thyroid nodules is a crucial subject, leading the decision for surgery. Ultrasonography and fine-needle aspiration cytology are pivotal examinations in the diagnostic process, with ultrasonography demonstrating better negative predictive value. Full article

Integrated sensing and communication (ISAC) is emerging as a main feature for 5G/6G communications. To enhance spectral and energy efficiencies in wireless environments, reconfigurable intelligent surfaces (RISs) will play a significant role in beyond-5G/6G communications. Multi-functional RISs, capable of not only reflecting or transmitting the beam in desired directions but also sensing the signal, wirelessly transferring power to nearby devices, harvesting energy, etc., will be highly beneficial for beyond-5G/6G applications. In this paper, we propose a nearly 2-bit unit cell of RISs integrated with sensing capabilities in the millimeter wave (mmWave) frequency band. To collect a very small fraction of the impinging signals through vias, we employed substrate integrated waveguide (SIW) technology at the bottom of the unit cell and a via. This enabled the sensing of incoming signals, requiring only a small amount of the impinging signal to be collected through SIW. Initially, we utilized Floquet ports and boundary conditions to obtain various parameters of the unit cells. Subsequently, we examined 1 × 3-unit cells, placing them on the waveguide model to obtain the required parameters of the unit cell. By using the waveguide and 1 × 3-unit cell arrays, the sensing amount was also determined. Full article

Rare earth elements (REEs) are a new type of material resource which have attracted significant attention in recent years. REEs have emerged as essential metals in modern-day technology due to their unique functions. The long-term, large-scale mining and utilization of rare earths has caused serious environmental pollution and constitutes a global health issue, which has raised concerns regarding the safety of human health. However, the toxicity profile of suspended particulate matter in REEs in the environment, which interacts with the human body, remains largely unknown. Studies have shown that REEs can enter the human body through a variety of pathways, leading to a variety of organ and system dysfunctions through changes in genetics, epigenetics, and signaling pathways. Through an extensive literature search and critical analysis, we provide a comprehensive overview of the available evidence, identify knowledge gaps, and make recommendations for future research directions. Full article

Against the backdrop of global environmental challenges and sustainable development goals, this paper pioneers the application of social network analysis to the study of spatial associations in China’s agricultural green development. It not only enhances the understanding of the spatial interconnectivity and network structural characteristics of agricultural green developments, but also captures the complex dependencies and interactions among provinces through a network lens, offering a fresh perspective on regional agricultural cooperation and competition. The study reveals: (1) The spatial network of China’s agricultural green development displays strong overall connectivity and enhanced stability, with regional green development trends becoming increasingly interlinked and interdependent. (2) The network exhibits a clear hierarchical and core-periphery structure which, over time, shows signs of diminishing, indicating a narrowing of developmental disparities among regions. (3) Significant shifts in the roles and positions of provinces within the network occur due to the relocation of industrial focal points and adjustments in development strategies, highlighting the complexity of dynamic changes among regions. (4) The spatial association network can be divided into four main clusters: Net spillover block, Bidirectional spillover block, Net beneficial block, and Broker block, with significant gradient characteristics in the relationships between these clusters, suggesting directional and differential flows and exchanges of resources and information among regions. (5) Geographic proximity, economic development level, informatization, and agricultural technological advancement significantly influenced the development and structural evolution of the network. Full article

Historically marginalized populations are susceptible to social isolation resulting from their unique social dynamics; thus, they incur a higher risk of developing chronic diseases across the course of life. Research has suggested that the cumulative effect of aging trajectories per se, across the lifespan, determines later-in-life disease risks. Emerging evidence has shown the biopsychosocial effects of social stress and social support on one’s wellbeing in terms of inflammation. Built upon previous multidisciplinary findings, here, we provide an overarching model that explains how the social dynamics of marginalized populations shape their rate of biological aging through the inflammatory process. Under the framework of social stress and social support theories, this model aims to facilitate our understanding of the biopsychosocial impacts of social dynamics on the wellbeing of historically marginalized individuals, with a special emphasis on biological aging. We leverage this model to advance our mechanistic understanding of the health disparity observed in historically marginalized populations and inform future remediation strategies. Full article

Vacuum saccharification significantly affected the flavor and color of preserved French plums. However, the correlation between color, flavor, and metabolites remains unclear. Metabolites contribute significantly to enhancing the taste and overall quality of preserved French plums. This study aimed to investigate the distinctive metabolites in samples from various stages of the processing of preserved French plums. The PCF4 exhibited the highest appearance, overall taste, and chroma. Furthermore, utilizing UPLC and ESI-Q TRAP-MS/MS, a comprehensive examination of the metabolome in the processing of preserved French plums was conducted. A total of 1776 metabolites were analyzed. Using WGCNA, we explored metabolites associated with sensory features through 10 modules. Based on this, building the correlation of modules and objective quantification metrics yielded three key modules. After screening for 151 differentiated metabolites, amino acids, and their derivatives, phenolic acids, flavonoids, organic acids, and other groups were identified as key differentiators. The response of differential metabolites to stress influenced the taste and color properties of preserved prunes. Based on these analyses, six important metabolic pathways were identified. This study identified changes in the sensory properties of sugar-stained preserved prunes and their association with metabolite composition, providing a scientific basis for future work to improve the quality of prune processing. Full article

This paper presents an approach to decolonial thinking and epistemological disobedience through what we call “decolonial cognitive triggers”. It is based on the struggles of urban peripheral communities in Brazil and explores eight triggers in the making of a Peripheral Epistemology. The unique points of our reflection are the sociocultural practices emerging from urban peripheral communities in Brazil and their responses to structural racism. As a part of this, we will explore the concepts of potência and convivência as core components of the decolonial debate, as well as their role in enabling epistemic disobedience in urban peripheries. We conclude by suggesting shifts in policy-making directed towards urban peripheries through the recognition and incorporation of such concepts and triggers. Full article

Suppressor of deltex (Su(dx)) is a Drosophila melanogaster member of the NEDD4 family of the HECT domain E3 ubiquitin ligases. Su(dx) acts as a regulator of Notch endocytic trafficking, promoting Notch lysosomal degradation and the down-regulation of both ligand-dependent and ligand-independent signalling, the latter involving trafficking through the endocytic pathway and activation of the endo/lysosomal membrane. Mutations of Su(dx) result in developmental phenotypes in the Drosophila wing that reflect increased Notch signalling, leading to gaps in the specification of the wing veins, and Su(dx) functions to provide the developmental robustness of Notch activity to environmental temperature shifts. The full developmental functions of Su(dx) are unclear; however, this is due to a lack of a clearly defined null allele. Here we report the first defined null mutation of Su(dx), generated by P-element excision, which removes the complete open reading frame. We show that the mutation is recessive-viable, with the Notch gain of function phenotypes affecting wing vein and leg development. We further uncover new roles for Su(dx) in Drosophila oogenesis, where it regulates interfollicular stalk formation, egg chamber separation and germline cyst enwrapment by the follicle stem cells. Interestingly, while the null allele exhibited a gain in Notch activity during oogenesis, the previously described Su(dx)^SP allele, which carries a seven amino acid in-frame deletion, displayed a Notch loss of function phenotypes and an increase in follicle stem cell turnover. This is despite both alleles displaying similar Notch gain of function in wing development. We attribute this unexpected context-dependent outcome of Su(dx)^sp being due to the partial retention of function by the intact C2 and WW domain regions of the protein. Our results extend our understanding of the developmental role of Su(dx) in the tissue renewal and homeostasis of the Drosophila ovary and illustrate the importance of examining an allelic series of mutations to fully understand developmental functions. Full article

The growth of electrical machine applications in high-torque environments such as marine propulsion and wind energy is encouraging the development of higher-power-density machines at ever higher efficiencies and under competitive pressure to meet higher demands. In this study, numerical simulations are performed to investigate the characteristics of air cooling applied to a 3 MW high-torque internal permanent magnet electric machine with integrated power electronics. The whole system of the main machine and two converters at either end are modelled with all details. Effects of different parameters on the total pressure drop and air flow rate to the machine and converters are examined. Results show that by changing the converter outlet hole size, the air flow rate to the machine and converter can be adjusted. Air guides and pin vents reveal excellent performance in the distribution of air to laminations and windings with a penalty of some increase in pressure drop, which is more pronounced when using smaller outlet holes. Furthermore, the air return manifold increases the pressure drop and causes a reduction in air flow rate to the converter. Insulation between compression plate and laminations is an unavoidable component used in electric machines and acts as a thermal insulator. However, it can also significantly augment pressure drop, especially in combination with smaller outlet holes. Thermal studies of the integrated power electronics illustrate that components’ temperatures are less than the temperature limit, confirming enough air through the converter. Analysis of power electronics in the case of fan failure provides the operational time window for the operators to respond. Full article

The use of osimertinib in the first-line (1L) setting is an effective treatment option for sensitizing EGFR-mutations (EGFRm+) and has significantly altered the standard of care practice for EGFRm+ disease in Canada. Unfortunately, acquired resistance to osimertinib is almost universal, and outcomes are disparate. Post-progression treatment patterns and the outcome of real-world Canadian EGFRm+ patients receiving 1L osimertinib were the focus of this retrospective review. Methods: The Glans-Look Lung Cancer Research database was used to identify and collect demographic, clinical, treatment, and outcome data on EGFRm+ patients who received 1L osimertinib in the Canadian province of Alberta between 2018 and 2022. Results: A total of 150 patients receiving 1L osimertinib were identified. In total, 86 developed progressive disease, with 56 (65%) continuing systemic therapy, 73% continuing osimertinib, and 27% switching to second-line (2L) systemic therapy. Patients were similar both in clinical characteristics at 1L osimertinib initiation and patterns of treatment failure at progression; those continuing 1L osimertinib post-progression had a longer time to progression (13.5 vs. 8.8 months, p = 0.05) and subsequent post-osimertinib initiation survival (34.7 vs. 22.8 months, p = 0.11). Conclusions: The continuation of osimertinib post-progression is an effective disease management strategy for select real-world EGFRm+ patients, providing continued clinical benefit, potentially due to different underlying disease pathogenesis. Full article

The influence of passenger gender, age, educational background, and other personal characteristics on satisfaction with an urban rail transit was studied. In total, 6340 valid questionnaires were completed, and basic data about the passengers were statistically analyzed. Based on AHP and the fuzzy comprehensive evaluation method, 94.594 percent of passengers reported overall satisfaction with the Qingdao rail transit; the data for subgroups based on gender, age, and other aspects were also calculated. An independent samples t-test and one-way analysis of variance were used to analyze the correlations between passenger satisfaction and the following parameters: gender, age, education, occupation, income, ride frequency, and private car availability. The results show that women attach more importance to the caring they feel in the process of travel than men, and no significant difference exists in travel satisfaction between passengers with private cars and those without private cars (p > 0.05). Older passengers report more satisfaction than younger passengers. Additionally, for passengers with high education and high income, satisfaction is lower in terms of safety, convenience, and comfort and caring. There are also significant differences in the safety, convenience, comfort, and caring experienced across different occupational groups. These research results provide a theoretical basis for understanding how passengers with different backgrounds perceive the operational services of an urban rail transit with regard to service defects, the weaknesses in the operation process, and passenger satisfaction. Full article

This study investigates the associations between countermovement rebound jump (CMRJ) performance metrics and various independent measures of athletic performance, including the isometric mid-thigh pull (IMTP), 20 m linear sprint, and 505 change-of-direction (COD) speed tests. Pearson’s correlations were used to analyse the relationship between the CMRJ measures with athletic performance, with significance being set at p ≤ 0.006. Results showed large significant positive relationships between IMTP peak force and force at 300 milliseconds with the first jump height of the CMRJ (JH-1, r = 0.54 to 0.55, p ≤ 0.002). Additionally, inverse relationships were observed between reactive strength index modified (RSImod) and reactive strength index (RSI) with 20 m sprint total and split times (r = −0.55 to −0.66, p ≤ 0.001), and the 10 m and total sprint times were significantly correlated with JH-1 (r = −0.54, p = 0.003), indicating that greater vertical explosive power and reactive strength are associated with faster sprint performance. Finally, a significant inverse relationship was identified between CMRJ metrics (two JH values and RSImod) and 505 COD times in both the left and right sides (r = −0.51 to −0.68, p ≤ 0.006). These findings suggest that CMRJ performance metrics are valuable indicators of lower-limb explosive force production, with a strong link to both linear sprint and COD performance. The finding underscores the importance of including CMRJ assessments in athletic performance evaluations due to their dual assessment capacity of slow and fast stretch–shortening cycle mechanics. Full article

The ionic toxicity induced by salinization has adverse effects on the growth and development of crops. However, researches on ionic toxicity and salt tolerance in plants have focused primarily on cations such as sodium ions (Na⁺), with very limited studies on chloride ions (Cl⁻). Here, we cloned the homologous genes of Arabidopsis thaliana AtCLCc, GhCLCc-1A/D, from upland cotton (Gossypium hirsutum), which were significantly induced by NaCl or KCl treatments. Subcellular localization showed that GhCLCc-1A/D were both localized to the tonoplast. Complementation of Arabidopsis atclcc mutant with GhCLCc-1 rescued its salt-sensitive phenotype. In addition, the silencing of the GhCLCc-1 gene led to an increased accumulation of Cl⁻ in the roots, stems, and leaves of cotton seedlings under salt treatments, resulting in compromised salt tolerance. And ectopic expression of the GhCLCc-1 gene in Arabidopsis reduced the accumulation of Cl⁻ in transgenic lines under salt treatments, thereby enhancing salt tolerance. These findings elucidate that GhCLCc-1 positively regulates salt tolerance by modulating Cl⁻ accumulation and could be a potential target gene for improving salt tolerance in plants. Full article