Using biodegradable films as a substitute for conventional polyolefin films has emerged as a crucial technology to combat agricultural white pollution. To address the shortcomings in the tensile strength, water vapor barrier properties, and degradation period of PBAT-based biodegradable films, this investigation aimed to create a composite film that could improve the diverse properties of PBAT films. To achieve this, a PBAT/PLA-PPC-PTLA ternary blend system was introduced in the study. The system effectively fused PBAT with PLA and PPC, as evidenced by electron microscopy tests showing no apparent defects on the surface and cross-section of the blended film. The developed ternary blend system resulted in a 58.62% improvement in tensile strength, a 70.33% enhancement in water vapor barrier properties, and a 30-day extension of the functional period compared to pure PBAT biodegradable films. Field experiments on corn crops demonstrated that the modified biodegradable film is more suitable for agricultural production, as it improved thermal insulation and moisture retention, leading to a 5.45% increase in corn yield, approaching the yield of traditional polyolefin films. Full article

Background: Chordomas pose a challenge in treatment due to their local invasiveness, high recurrence, and potential lethality. Despite being slow-growing and rarely metastasizing, these tumors often resist conventional chemotherapies (CTs) and radiotherapies (RTs), making surgical resection a crucial intervention. However, achieving radical resection for chordomas is seldom possible, presenting therapeutic challenges. The accurate diagnosis of these tumors is vital for their distinct prognoses, yet differentiation is hindered by overlapping radiological and histopathological features. Fortunately, recent molecular and genetic studies, including extracranial location analysis, offer valuable insights for precise diagnosis. This literature review delves into the genetic aberrations and molecular biology of chordomas, aiming to provide an overview of more successful therapeutic strategies. Methods: A systematic search was conducted across major medical databases (PubMed, Embase, and Cochrane Library) up to 28 January 2023. The search strategy utilized relevant Medical Subject Heading (MeSH) terms and keywords related to “chordomas”, “molecular biology”, “gene aberrations”, and “target therapies”. The studies included in this review consist of preclinical cell studies, case reports, case series, randomized controlled trials, non-randomized controlled trials, and cohort studies reporting on genetic and biological aberrations in chordomas. Results: Of the initial 297 articles identified, 40 articles were included in the article. Two tables highlighted clinical studies and ongoing clinical trials, encompassing 18 and 22 studies, respectively. The clinical studies involved 185 patients diagnosed with chordomas. The tumor sites were predominantly sacral (n = 8, 44.4%), followed by clivus (n = 7, 38.9%) and lumbar spine (n = 3, 16.7%). Primary treatments preceding targeted therapies included surgery (n = 10, 55.6%), RT (n = 9, 50.0%), and systemic treatments (n = 7, 38.9%). Various agents targeting specific molecular pathways were analyzed in the studies, such as imatinib (a tyrosine kinase inhibitor), erlotinib, and bevacizumab, which target EGFR/VEGFR. Common adverse events included fatigue (47.1%), skin reactions (32.4%), hypertension (23.5%), diarrhea (17.6%), and thyroid abnormalities (5.9%). Clinical outcomes were systematically assessed based on progression-free survival (PFS), overall survival (OS), and tumor response evaluated using RECIST or CHOI criteria. Notably, stable disease (SD) occurred in 58.1% of cases, and partial responses (PRs) were observed in 28.2% of patients, while 13.7% experienced disease progression (PD) despite targeted therapy. Among the 22 clinical trials included in the analysis, Phase II trials were the most prevalent (40.9%), followed by I-II trials (31.8%) and Phase I trials (27.3%). PD-1 inhibitors were the most frequently utilized, appearing in 50% of the trials, followed by PD-L1 inhibitors (36.4%), CTLA-4 inhibitors (22.7%), and mTOR inhibitors (13.6%). Conclusions: This systematic review provides an extensive overview of the state of targeted therapy for chordomas, highlighting their potential to stabilize the illness and enhance clinical outcomes. Full article

Escalating global climate change and the intensification of urban heatwaves have led to an increase in summer air conditioning cooling energy consumption. This phenomenon is particularly critical in tropical regions, as it may trigger an energy crisis. The rational setting of indoor thermal design parameters can help conserve energy to the maximum extent while ensuring thermal comfort for occupants. This study selected Haikou City, a unique tropical city in China, as the research location. Indoor environment measurements and a questionnaire survey were conducted with participants, and the outdoor thermal environment sensitivity, population attributes and differences in thermal sensation, thermal neutral temperature, and comfort range were calculated and analyzed. The following results were obtained. Based on the overall population, long-term residence, and temporary residence classification, the indoor thermal comfort needs of residents in tropical cities in Haikou were effectively identified. The actual thermal neutral temperature of the overall population is 25.7 °C, and 90% of the acceptable thermal comfort temperature range is 23.2 °C–28.0 °C. The actual thermal neutral temperature of the regular residents is 27.3 °C, and 90% of the acceptable thermal comfort temperature range is 23.3 °C–31.4 °C. The actual thermal neutral temperature of the temporary population is 25.5 °C, and 90% of the acceptable thermal comfort temperature range is 23.0 °C–28.0 °C. These research results have an important reference value for improving the setting of the temperature of air conditioning in tropical areas in summer and further reducing energy consumption, which is conducive to sustainable development. Full article

Cupressus gigantea W. C. Cheng & L. K. Fu is an endemic conifer tree species that is distributed widely along the northern portion of the deep gorge of the Yarlung Tsangbo River on the Tibetan Plateau. However, as a key plant species growing on the Tibetan plateau, C. gigantea has since become an endangered species due to habitat loss and degradation, overexploitation, and other factors. It has been listed as a first-grade national protected wild plant species in China. Accordingly, to conserve this plant species, we should obtain more information on its genetic structure. In this study, the genetic diversity and structure among 67 samples were evaluated by the inter-simple sequence repeat (ISSR) technique. Overall, 78 bands were produced with a molecular length of 200 bp to 3100 bp using 10 ISSR primers. The mean values for the average number of alleles (Na), effective number of alleles (Ne), Nei’s gene diversity (H), and Shannon’s information index (I) were 1.529, 1.348, 0.199, and 0.293, respectively. Additionally, the number of polymorphic loci (NPLs) and percentage of polymorphic loci (PPLs) averaged 41.25 and 52.90, respectively. Further, total variation among populations was 14.2%, while that within populations was 85.8%; accordingly, the within-population genetic differentiation was found to be significant (p < 0.001). These results demonstrated that a genetic structure model with K = 3 fitted the data best, which agreed with the unweighted pair group method with arithmetic average (UPGMA) cluster and the principal coordinate analysis (PCoA). These findings are beneficial for ensuring the development and genetic protection of C. gigantea populations in the future. Full article

Hypoxia is a condition in which tissues of the body do not receive sufficient amounts of oxygen supply. Numerous studies have elucidated the intricate roles of hypoxia and its involvement in both physiological and pathological conditions. This study aimed to clarify the impact of a forced low-oxygen environment in early pregnancy by exposing mice to low-oxygen conditions for 24–72 h after fertilization. The treatment resulted in the complete failure of blastocyst implantation, accompanied by vascular hyperpermeability in the uterus. A transcriptome analysis of the uterus revealed remarkable alterations in gene expression between control normoxic- and hypoxic-treatment groups. These alterations were characterized by the differentially expressed genes categorized into the immune responses and iron coordination. Furthermore, exposure to a low-oxygen environment caused apoptosis in the corpus luteum within the ovary and a reduction in progesterone secretion. Consequently, diminished plasma progesterone levels were considered to contribute to implantation failure in combination with the activation of the hypoxic pathway in the uterus. Additionally, previous studies have demonstrated the impact of hypoxic reactions on blastocyst development and the pre-implantation process in the endometrium. Our findings suggest that the corpus luteum exhibits elevated susceptibility to hypoxia, thereby elucidating a critical aspect of its physiological response. Full article

The escalating levels of atmospheric CO₂, primarily attributed to human activities, underscore the urgent need for innovative solutions to mitigate environmental challenges. This study delves into the electrochemical reduction of CO₂ as a promising avenue for sustainable carbon capture and utilization. Focused on the formation of formate (HCOO⁻/HCOOH), a high-value product, the research explores the integration of nonthermal plasma (NTP) with electrochemical processes—an approach rarely studied in existing literature. A comprehensive investigation involves varying parameters such as plasma discharging voltage, carrier gas, discharging mode, electrolysis voltage, polarity, and plasma type. The electrochemical tests employ a 10 wt.% Pd/C catalyst, and formate production is quantitatively analyzed using NMR. Results reveal that NTP significantly enhances CO₂ reduction, with key factors influencing formate yield elucidated. The study reveals the complexity of CO₂ electrochemical reduction, providing novel insights into the synergistic effects of NTP. These findings contribute to advancing sustainable technologies for CO₂ utilization, paving the way for more efficient and environmentally friendly processes in the pursuit of a carbon-neutral future. Full article

The aim of the of the current investigation was to investigate the possible differences concerning males and females in anthropometry, body composition, functional capacity, strength and quality of life variables. After obtaining signed informed consent, 37 participants (18 males; 19 females), with mean age of 39.08 and standard deviation of 11.66 years, voluntarily participated in this study. Anthropometry, body composition, functional capacity, strength, and quality of life were assessed using validated and reliable instruments and tests for this population. The males and females were compared using a Mann–Whitney U signed rank test. Significant differences were detected among the following variables, height (p = 0.028), body mass index (p = 0.033), fat mass (p = 0.002), muscle mass (p ≤ 0.001), phase angle (p = 0.005), medicine ball throwing strength (p = 0.010), and peak toque left knee (p = 0.028), with males showing better results in all the variables. The sample in this study showed differences in the anthropometric, composition, and strength variables. Studying this population can help ensure that everyone has equal access to services and adequate support for their personal needs, improving their quality of life. Full article

Ransomware poses a significant threat by encrypting files or systems demanding a ransom be paid. Early detection is essential to mitigate its impact. This paper presents an Uncertainty-Aware Dynamic Early Stopping (UA-DES) technique for optimizing Deep Belief Networks (DBNs) in ransomware detection. UA-DES leverages Bayesian methods, dropout techniques, and an active learning framework to dynamically adjust the number of epochs during the training of the detection model, preventing overfitting while enhancing model accuracy and reliability. Our solution takes a set of Application Programming Interfaces (APIs), representing ransomware behavior as input we call “UA-DES-DBN.” The method incorporates uncertainty and calibration quality measures, optimizing the training process for better more accurate ransomware detection. Experiments demonstrate the effectiveness of UA-DES-DBN compared to more conventional models. The proposed model improved accuracy from 94% to 98% across various input sizes, surpassing other models. UA-DES-DBN also decreased the false positive rate from 0.18 to 0.10, making it more useful in real-world cybersecurity applications. Full article

The classification of roadway contexts and speeds is a critical step in the planning, design, and operation of highway infrastructure. In developing countries, road users encounter safety and operational issues due to poorly defined roadway contexts and inappropriately determined target speeds for a highway network. This study developed an expert system for classifying roadway contexts and target speeds of multilane highway segments and applied the classification process to 16,235 km of multilane highways in Thailand’s highway network. The proposed methodology used a fuzzy decision mechanism to deal with subjective and imprecise expert judgment (e.g., low, high), many variables, and a complex evaluation process. This study used the Fuzzy Delphi method to identify the possible important factors influencing contexts and speeds and the Fuzzy Inference System method to reason factors to categorize multilane highway segments in Thailand into different classes of roadway contexts (e.g., rural, low-density suburban, high-density suburban, and urban highways) and target speeds (e.g., ≤50 km/h, 50–60 km/h, 60–70 km/h, 70–80 km/h, 80–90 km/h, 90–100 km/h, and 100 km/h). The study was based on data from questionnaire surveys of experts and field investigations of 120 highway segments. The results showed that roadside environments and activities influence the roadway contexts, while the target speeds are sensitive to the roadway characteristics and contexts. These findings support the need for changes in context-adapted highway design and speed management. The proposed expert system provided high accuracy (90.8%) in classifications of both roadway contexts and target speeds. The fuzzy expert system provides a systematic and structural framework for analyzing imprecise data in highway contextual and speed classifications and improving the clarity and accuracy of the evaluation process. The implementation of the fuzzy expert system has the potential to revolutionize the highway classification decision-making problem under uncertainty. Full article

In order to rapidly breed high-quality varieties, an increasing number of plant researchers have identified the functions of a large number of genes, but there is a serious lack of research on plants’ phenotypic traits. This severely hampers the breeding process and exacerbates the dual challenges of scarce resources and resource development and utilization. Currently, research on crop phenotyping has gradually transitioned from traditional methods to HTP technologies, highlighting the high regard scientists have for these technologies. It is well known that different crops’ phenotypic traits exhibit certain differences. Therefore, in rapidly acquiring phenotypic data and efficiently extracting key information from massive datasets is precisely where HTP technologies play a crucial role in agricultural development. The core content of this article, starting from the perspective of crop phenomics, summarizes the current research status of HTP technology, both domestically and internationally; the application of HTP technology in above-ground and underground parts of crops; and its integration with precision agriculture implementation and multi-omics research. Finally, the bottleneck and countermeasures of HTP technology in the current agricultural context are proposed in order to provide a new method for phenotype research. HTP technologies dynamically monitor plant growth conditions with multi-scale, comprehensive, and automated assessments. This enables a more effective exploration of the intrinsic “genotype-phenotype-environment” relationships, unveiling the mechanisms behind specific biological traits. In doing so, these technologies support the improvement and evolution of superior varieties. Full article

In this work, an experimental analysis of the performance of a cross-flow turbine, commonly referred to as a Michell–Banki turbine (MBT), is carried out for small-scale hydropower production in rural areas located in developing countries to support their social and economic development activities. The study investigates how the efficiency of the MBT is influenced by the presence or absence of a nozzle, along with variations in the internal guide vane (GV) and its angle. The runner had 26 blades that were arranged symmetrically in the periphery between two circular plates. The designed MBT had the ability to generate a maximum of 100 W of power at a water flow rate and a head of 0.009 m³/s and 0.6311 m, respectively. The experimental tests were carried out using a hydraulic bench. The turbine efficiency without the inner GV was found to be higher than that of the turbine with the inner GV; i.e., it was found that the utilization of the GV did not enhance the efficiency of the MBT due to the occurrence of a choking effect. A maximum hydraulic efficiency of 85% was achieved in the turbine without an inner GV in comparison with the efficiency achieved (77%) with this device and an optimum opening angle of the GV of 24° (75% of opening). In this regard, the GV design should be carefully carried out to improve the MBT efficiency. Additionally, the effect of the GV shape on the MBT performance should be experimentally investigated to obtain a more general judgment regarding the role of this device. Full article

For steel–concrete composite bridges, the cracking of concrete in the tensile zone influences the serviceability of bridges and decreases their durability. UHPC, as a high-tensile and -durability material, is used to replace a part of the concrete to enhance the tensile performance. Thus, the steel–normal concrete–UHPC composite slab, as a new composite structure, is formed. This paper investigates the flexural behaviours of steel–normal concrete–UHPC composite slabs through a full-scale experiment, numerical simulation, and theoretical analysis. The research results indicate that (1) UHPC enhances the flexural performance of the tensile zone and delays the development of cracks. The initial cracking force of concrete increases from 44 kN to 91 kN. (2) UHPC effectively enhances the carrying capacity of composite slabs. A 50 mm UHPC layer makes the flexural bearing capacity of steel–concrete composite slabs increase by 13.51%. (3) The construction methods influence the initial cracking force of composite slabs. For full-span scaffolding construction, the initial cracking force decreases from 91 kN to 69 kN compared with construction without brackets. (4) The theoretical model considering the tensile contribution of cracking UHPC can accurately predict the bearing capacity of the composite slabs. And the theoretical values of the bearing capacity are lower than the experimental values, which makes the composite slabs safer in service. Full article

Inkjet printing technology offers a unique approach to producing direct-patterned pixels without fine metal masks for active matrix displays. Organic light-emitting diodes (OLEDs) consisting of thermally activated delayed fluorescence (TADF) emitters facilitate efficient light emission without heavy metals, such as platinum and iridium. Multi-resonance TADF molecules, characterized by their small full width at half maxima (FWHM), are highly suitable for the requirements of wide color-gamut displays. Herein, host-free TADF inks with a low concentration of 1 mg/mL were developed and inkjet-printed onto a seeding layer, concurrently serving as the hole-transporting layer. Attributed to the proof-of-concept of host-free inks printed on a mixed seeding layer, a maximum external quantum efficiency of 13.1% (improved by a factor of 21.8) was achieved in the inkjet-printed OLED, with a remarkably narrow FWHM of only 32 nm. Highly efficient energy transfer was facilitated by the effective dispersion of the sensitizer around the terminal emitters. Full article

Copper-catalyzed azide–alkyne cycloaddition click (CuAAC) reaction is widely used to synthesize drug candidates and other biomolecule classes. Homogeneous catalysts, which consist of copper coordinated to a ligand framework, have been optimized for high yield and specificity of the CuAAC reaction, but CuAAC reaction with these catalysts requires the addition of a reducing agent and basic conditions, which can complicate some of the desired syntheses. Additionally, removing copper from the synthesized CuAAC-containing biomolecule is necessary for biological applications but inconvenient and requires additional purification steps. We describe here the design and synthesis of a PNN-type pincer ligand complex with copper (I) that stabilizes the copper (I) and, therefore, can act as a CuAAC catalyst without a reducing agent and base under physiologically relevant conditions. This complex was immobilized on two types of resin, and one of the immobilized catalyst forms worked well under aqueous physiological conditions. Minimal copper leaching was observed from the immobilized catalyst, which allowed its use in multiple reaction cycles without the addition of any reducing agent or base and without recharging with copper ion. The mechanism of the catalytic cycle was rationalized by density functional theory (DFT). This catalyst’s utility was demonstrated by synthesizing coumarin derivatives of small molecules such as ferrocene and sugar. Full article

Several studies have shown an inverse correlation between the likelihood of developing a neurodegenerative disorder and cancer. We previously reported that the levels of amyloid beta (Aβ), at the center of Alzheimer’s disease pathophysiology, are regulated by acetylcholinesterase (AChE) in non-small cell lung cancer (NSCLC). Here, we examined the effect of Aβ or its fragments on the levels of ACh in A549 (p53 wild-type) and H1299 (p53-null) NSCLC cell media. ACh levels were reduced by cell treatment with Aβ 1–42, Aβ 1–40, Aβ 1–28, and Aβ 25–35. AChE and p53 activities increased upon A549 cell treatment with Aβ, while knockdown of p53 in A549 cells increased ACh levels, decreased AChE activity, and diminished the Aβ effects. Aβ increased the ratio of phospho/total p38 MAPK and decreased the activity of PKC. Inhibiting p38 MAPK reduced the activity of p53 in A549 cells and increased ACh levels in the media of both cell lines, while opposite effects were found upon inhibiting PKC. ACh decreased the activity of p53 in A549 cells, decreased p38 MAPK activity, increased PKC activity, and diminished the effect of Aβ on those activities. Moreover, the negative effect of Aβ on cell viability was diminished by cell co-treatment with ACh. Full article

Assessment methodologies such as BREEAM and LEED allocate points based on prescribed interventions that promote design features or strategies considered to be more sustainable than others. A focus on accumulating numerical scores, however, often fails to address pertinent contextual issues, particularly within developing countries. This paper examines the use of four assessment systems in Kenya—two international systems, LEED-US and Green Star SA-Kenya; and two locally developed systems, Green Mark Kenya and the Safari Green Building Index. The paper compares the relative weighting of different categories under each system, and assesses their appropriateness to a Kenyan context, with reference to the suitability of active technology versus passive design approaches. The paper examines selected examples of ‘green’ buildings in Nairobi, reflecting on the influence of different methods of assessment on the adopted design approaches. The paper argues that international rating systems, such as LEED, often focus on a Western construct of sustainability featuring a systematic bias towards global rather than local perspectives, with an emphasis on physical environmental factors. In pursuit of objectivity, the measurement of non-contextual parameters untailored to local circumstances (e.g., energy performance) is prioritised at the expense of those contingent on local conditions or climate. Full article

Through the analysis of various typical cases, this study examines whether the establishment of an agro-product regional public brand (ARPB) can effectively boost the revenue of farmers and increase their share in the supply chain. The findings suggest that an early-stage ARPB can command a price premium for products, yet its overall contribution to farmers’ revenue remains limited due to scale constraints. The premium ability of an ARPB is influenced by product characteristics and sales strategies, underscoring the need to enhance control over terminal sales. Although the revenue of all operators in an ARPB supply chain shows an increase compared to that of a non-ARPB supply chain, the ratios of revenue allocated to farmers diminish. The Shapley value method was utilized to optimize the revenue-sharing in the supply chain, indicating a need to increase the share of revenue for farmers. This optimization necessitates the formation of a community of interests between farmers, processing enterprises, and sellers to facilitate the upstream movement of brand premiums. Furthermore, enhancing the government’s mediation and regulatory functions can provide farmers with more opportunities to partake in brand benefits. Full article

Nicotinamide adenine dinucleotide (NAD) is one of the most important and essential components within an organism. Extensive ongoing research is aimed at harnessing its potential in managing diverse diseases by supplying various forms of NAD in its oxidized state, NAD⁺. Ultraviolet radiation (UVR) is the most common environmental exposure factor, but also carries many risks. UVR affects the epidermis and contributes to sunburn, photo-allergy, DNA damage, and certain cancers, notably melanoma. Research has shown that NAD⁺ precursors, including nicotinamide riboside (NR), reduce melanogenesis in aged melanocytes. In this study, we used NR to determine whether melanin hyperpigmentation was suppressed after light stimulation. We found that melanogenesis was inhibited when B16F10 cells treated with α-melanocyte-stimulating hormone were exposed to specific doses of NR. Additionally, tyrosinase activity (a key step in melanin production) was suppressed. However, there was no difference in the expression level of melanogenic genes. Ultraviolet B light directly stimulated HaCaT cells, inducing the RNA expression of metalloproteinases. Treatment with NR suppressed the corresponding gene expression and reduced cytotoxicity. This study demonstrates the possibility of using NR as a new skin-whitening ingredient due to its inhibitory effect on hyperpigmentation and ability to maintain skin layers affected by UVR. Full article

Chinese patients face higher risks of gastrointestinal (GI) cancers and greater cancer-related deaths than Canadian-born patients. The older population encounters barriers to quality healthcare, impacting their well-being and survival. Previous studies highlighted Chinese immigrant perceptions of not requiring healthcare support. During the COVID-19 pandemic, their underutilization of healthcare services garnered attention. The present study explores the experiences of older Chinese cancer patients to improve culturally sensitive cancer care. A total of twenty interviews carried out in Cantonese and Mandarin were conducted with Chinese immigrants, aged 60 or above, diagnosed with Stage 3 or 4 GI cancer. These interviews were transcribed verbatim, translated, and subjected to qualitative descriptive analysis. Among older Chinese immigrant patients, a phenomenon termed “Premature Acceptance: Normalizing Death and Dying” was observed. This involved four key themes: 1. acceptance and letting go, 2. family first, 3. self-sufficiency, and 4. barriers to supportive care. Participants displayed an early acceptance of their own mortality, prioritizing family prosperity over their own quality of life. Older Chinese patients normalize the reality of facing death amidst cancer. They adopt a pragmatic outlook, acknowledging life-saving treatments while willingly sacrificing their own support needs to ease family burdens. Efforts to enhance health literacy require culturally sensitive programs tailored to address language barriers and differing values among this population. A strengths-based approach emphasizing family support and practical aspects of care may help build resilience and improve symptom management, thereby enhancing their engagement with healthcare services. Full article

Detection and characterization of hidden defects, impurities, and damages in homogeneous materials like aluminum die casting materials, as well as composite materials like Fiber–Metal Laminates (FML), is still a challenge. This work discusses methods and challenges in data-driven modeling of automated damage and defect detectors using measured X-ray single- and multi-projection images. Three main issues are identified: Data and feature variance, data feature labeling (for supervised machine learning), and the missing ground truth. It will be shown that simulation of synthetic measuring data can deliver a ground truth dataset and accurate labeling for data-driven modeling, but it cannot be used directly to predict defects in manufacturing processes. Noise has a significant impact on the feature detection and will be discussed. Data-driven feature detectors are implemented with semantic pixel Convolutional Neural Networks. Experimental data are measured with different devices: A low-quality and low-cost (Low-Q) X-ray radiography, a typical industrial mid-quality X-ray radiography and Computed Tomography (CT) system, and a state-of-the-art high-quality μ-CT device. The goals of this work are the training of robust and generalized data-driven ML feature detectors with synthetic data only and the transition from CT to single-projection radiography imaging and analysis. Although, as the title implies, the primary task is pore characterization in aluminum high-pressure die-cast materials, but the methods and results are not limited to this use case. Full article

Scorpion venom peptides are generally classified into two main groups: the disulfide bridged peptides (DBPs), which usually target membrane-associated ion channels, and the non-disulfide bridged peptides (NDBPs), a smaller group with multifunctional properties. In the past decade, these peptides have gained interest because most of them display functions that include antimicrobial, anticancer, haemolytic, and anti-inflammatory activities. Our current study focuses on the short (9–19 amino acids) antimicrobial linear scorpion peptides. Most of these peptides display a net positive charge of 1 or 2, an isoelectric point at pH 9–10, a broad range of hydrophobicity, and a Grand Average of Hydropathy (GRAVY) Value ranging between −0.05 and 1.7. These features allow these peptides to be attracted toward the negatively charged phospholipid head groups of the lipid membranes of target cells, a force driven by electrostatic interactions. This review outlines the antimicrobial potential of short-chained linear scorpion venom peptides. Additionally, short linear scorpion peptides are in general more attractive for large-scale synthesis from a manufacturing point of view. The structural and functional diversity of these peptides represents a good starting point for the development of new peptide-based therapeutics. Full article

Fish in aquatic ecosystems are often impacted by environmental stressors like temperature fluctuations and exposure to heavy metals. Chromium (Cr⁶⁺) is a known environmental pollutant that poses a threat to aquatic life. Various environmental factors, such as water temperature, have been found to affect the toxicity of dissolved chemicals in aquatic ecosystems. We investigated the toxicity of combinations of different concentrations of hexavalent chromium (Cr⁶⁺) with high temperatures in fish. Hematological indices demonstrated changes in white blood cells (WBCs), hematocrit (HCT), red blood cells (RBCs), and hemoglobin (Hb) levels during the exposure. The qualitative and semi-quantitative analyses of different tissues confirmed that higher concentrations of Cr⁶⁺ caused more significant damage than lower concentrations, with evident alterations observed in circulatory and regressive aspects. Furthermore, brain acetylcholinesterase levels decreased in both single heavy metal exposure and combined exposure at a high temperature. The activity of antioxidant oxidase and immunological parameters increased in all treatment groups compared with the control group following long-term exposure. A significant and increased effect of Cr⁶⁺ in the high-temperature groups was observed on the evaluated biomarkers, suggesting a possible synergistic effect between Cr⁶⁺ and increased temperature. The integrated biomarker response (IBR) reported the highest level of stress at 10 mg/L Cr⁶⁺ combined with high temperature. The IBR analysis revealed that the highest activity of response enzymes, such as acid phosphatase (ACP), superoxide dismutase (SOD), and glutathione S-transferases (GST), was observed in the liver, whereas the gills displayed alkaline phosphatase (ALP), GST, and SOD activity, and the kidneys demonstrated SOD, ACP, and aspartate aminotransferase (AST) to be most active. Through histopathology, antioxidant enzymes, and metabolism- and immunity-related enzymes, we determined that high temperatures enhance the potential toxicity of Cr⁶⁺ in fish. We recommend conducting a thorough assessment of the impact of climate change, particularly temperature fluctuations, when studying the toxic effects of metal pollution, like chromium, in aquatic ecosystems. Full article