The 2023 MDPI Annual Report has
been released!
 
13 pages, 3398 KiB  
Article
Interactions between Root Hair Development and Arbuscular Mycorrhizal Fungal Colonization in Trifoliate Orange Seedlings in Response to P Levels
by Xiu Cao, Yu Zhao, Ren-Xue Xia, Qiang-Sheng Wu, Abeer Hashem and Elsayed Fathi Abd_Allah
Agriculture 2024, 14(5), 763; https://doi.org/10.3390/agriculture14050763 (registering DOI) - 15 May 2024
Abstract
Both arbuscular mycorrhizal (AM) fungi and root hairs are crucial in facilitating plant uptake of phosphorus (P), while it is unclear whether and how they respond to varying P supplies. In order to explore how AM fungal colonization and root hair development are [...] Read more.
Both arbuscular mycorrhizal (AM) fungi and root hairs are crucial in facilitating plant uptake of phosphorus (P), while it is unclear whether and how they respond to varying P supplies. In order to explore how AM fungal colonization and root hair development are affected by substrate P supply, trifoliate orange (Poncirus trifoliata) seedlings were inoculated with AM fungus Rhizophagus intraradices and grown under low, moderate, and high P conditions; then, root hair morphological features and AM fungal colonization were measured. Following 120 days of AM fungal inoculation, root hair density, root hair length, AM fungal colonization rate, arbuscule colonization rate, and AM fungal colonization frequency all increased significantly under P-deficient conditions but decreased under high P conditions. Moreover, the colonization of AM fungi had a major impact on root hair formation by altering the expression of related genes and the growth of epidermal cells. The effect of AM fungi was dependent on P supply levels, as evidenced by the fact that root hair density and length increased at high P levels but decreased at low P levels. As a result, root hairs may serve as a preferential site for AM fungal colonization, and their morphology could influence the early stage of AM symbiosis establishment. Full article
(This article belongs to the Special Issue Arbuscular Mycorrhiza in Cropping Systems)
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12 pages, 390 KiB  
Article
Understanding the Impact of the Nurse Manager’s Vocation for Leadership on the Healthcare Workplace Environments in Mexico: A Grounded Theory Approach
by Angeles Yañez-Lozano and Manuel Lillo-Crespo
Nurs. Rep. 2024, 14(2), 1224-1235; https://doi.org/10.3390/nursrep14020093 (registering DOI) - 15 May 2024
Abstract
Background: Leadership in any managerial position that a nurse may hold appears to be closely connected to fostering positive and productive work environments within healthcare settings. However, not all nurse managers are characterized by leadership, and not all nurse leaders are nurse managers. [...] Read more.
Background: Leadership in any managerial position that a nurse may hold appears to be closely connected to fostering positive and productive work environments within healthcare settings. However, not all nurse managers are characterized by leadership, and not all nurse leaders are nurse managers. In countries, such as Mexico, those who occupy these roles have barely sufficient training in management, are mainly characterized by their experience in one clinical specialty and their vocation for leadership is not a requirement. Our study aims to understand how the leadership vocation that some nurse managers have in their daily practice could impact the work environments of healthcare organizations in Mexico. Methods: A qualitative design was carried out through a grounded theory approach based on Corbin & Strauss, interviewing individually 13 nurse managers with representative experience in Mexican public sector hospitals. Results: According to the interviewees, the meaning of vocation is based mainly on the love for their profession, on the vocation of being a nurse and caring for people, as well as on the development of their own natural leadership potential for decision-making in practice. This situation produces positive feedback on themselves by generating productive effects in the work environment, consequently strengthening them to better organize professional resources and therefore producing improvements. Conclusions: The nurse manager’s vocation of leadership derives from both the vocation of being a practicing nurse and the vocation for being a leader, positively impacting and productively contributing to the improvement of the work environment. Consequently, nurse managers with vocation for leadership should be the gold standard role in any healthcare organization. This study was retrospectively registered with the (registros-OSF-bmyvz-v1) on the (26 July 2023) registration number (10.17605/OSF.IO/BMYVZ). Full article
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14 pages, 994 KiB  
Systematic Review
Botulinum Toxin for Axial Postural Abnormalities in Parkinson’s Disease: A Systematic Review
by Marialuisa Gandolfi, Carlo Alberto Artusi, Gabriele Imbalzano, Serena Camozzi, Mauro Crestani, Leonardo Lopiano, Michele Tinazzi and Christian Geroin
Toxins 2024, 16(5), 228; https://doi.org/10.3390/toxins16050228 (registering DOI) - 15 May 2024
Abstract
Axial postural abnormalities (APAs), characterized by their frequency, disabling nature, and resistance to pharmacological treatments, significantly impact Parkinson’s disease and atypical Parkinsonism patients. Despite advancements in diagnosing, assessing, and understanding their pathophysiology, managing these complications remains a significant challenge. Often underestimated by healthcare [...] Read more.
Axial postural abnormalities (APAs), characterized by their frequency, disabling nature, and resistance to pharmacological treatments, significantly impact Parkinson’s disease and atypical Parkinsonism patients. Despite advancements in diagnosing, assessing, and understanding their pathophysiology, managing these complications remains a significant challenge. Often underestimated by healthcare professionals, these disturbances can exacerbate disability. This systematic review assesses botulinum toxin treatments’ effectiveness, alone and with rehabilitation, in addressing APAs in Parkinson’s disease, utilizing MEDLINE (PubMed), Web of Science, and SCOPUS databases for source material. Of the 1087 records retrieved, 16 met the selection criteria. Most research has focused on botulinum toxin (BoNT) as the primary treatment for camptocormia and Pisa syndrome, utilizing mostly observational methods. Despite dose and injection site variations, a common strategy was using electromyography-guided injections, occasionally enhanced with ultrasound. Patients with Pisa syndrome notably saw consistent improvements in APAs and pain. However, studies on the combined effects of botulinum toxin and rehabilitation are limited, and antecollis is significantly under-researched. These findings recommend precise BoNT injections into hyperactive muscles in well-selected patients by skilled clinicians, avoiding compensatory muscles, and underscore the necessity of early rehabilitation. Rehabilitation is crucial in a multidisciplinary approach to managing APAs, highlighting the importance of a multidisciplinary team of experts. Full article
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11 pages, 578 KiB  
Article
Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate
by Ana Maria Pintão, Tiago Santos and Fátima Nogueira
Molecules 2024, 29(10), 2316; https://doi.org/10.3390/molecules29102316 (registering DOI) - 15 May 2024
Abstract
Malaria remains an important and challenging infectious disease, and novel antimalarials are required. Benzyl isothiocyanate (BITC), the main breakdown product of benzyl glucosinolate, is present in all parts of Tropaeolum majus L. (T. majus) and has antibacterial and antiparasitic activities. To [...] Read more.
Malaria remains an important and challenging infectious disease, and novel antimalarials are required. Benzyl isothiocyanate (BITC), the main breakdown product of benzyl glucosinolate, is present in all parts of Tropaeolum majus L. (T. majus) and has antibacterial and antiparasitic activities. To our knowledge, there is no information on the effects of BITC against malaria. The present study evaluates the antimalarial activity of aqueous extracts of BITC and T. majus seeds, leaves, and stems. We used flow cytometry to calculate the growth inhibition (GI) percentage of the extracts and BITC against unsynchronized cultures of the chloroquine-susceptible Plasmodium falciparum 3D7 − GFP strain. Extracts and/or compounds with at least 70% GI were validated by IC50 estimation against P. falciparum 3D7 − GFP and Dd2 (chloroquine-resistant strain) unsynchronized cultures by flow cytometry, and the resistance index (RI) was determined. T. majus aqueous extracts showed some antimalarial activity that was higher in seeds than in leaves or stems. BITC’s GI was comparable to chloroquine’s. BITC’s IC50 was similar in both strains; thus, a cross-resistance absence with aminoquinolines was found (RI < 1). BITC presented features that could open new avenues for malaria drug discovery. Full article
(This article belongs to the Special Issue The Design, Synthesis, and Biological Activity of New Drug Candidates)
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8 pages, 7792 KiB  
Article
Visualization of Effectiveness: The Use of a Set of Colored Cleaning Wipes for Visible Disinfection of Ultrasound Probes
by Christian Kühnel and Falk Gühne
Hygiene 2024, 4(2), 189-196; https://doi.org/10.3390/hygiene4020015 (registering DOI) - 15 May 2024
Abstract
The German health authorities’ guidelines for medical devices in 2012 highlighted the importance of cleaning ultrasound probes, emphasizing their validation and reliability. In addition to automated and validated options, alternative manual methods such as wipe disinfection have gained traction due to their independence [...] Read more.
The German health authorities’ guidelines for medical devices in 2012 highlighted the importance of cleaning ultrasound probes, emphasizing their validation and reliability. In addition to automated and validated options, alternative manual methods such as wipe disinfection have gained traction due to their independence from additional hardware. The study examines the effectiveness of a manual cleaning process using wipes, addressing concerns raised by the Robert Koch Institute regarding the lack of validation for wipe disinfection of semi-critical devices. The EQUINOS colored wipe disinfection kit identified wetting gaps in all cleanings across four probes tested. The results indicate significant challenges in ensuring complete surface wetting, particularly in complex device parts such as clip-on areas and fixtures for additional biopsy attachments, suggesting that manual methods alone may not adequately mitigate the risk of infection transmission (p value < 0.0001). The study concludes that while manual disinfection methods are a commonly used alternative to automated reprocessing, there is a critical need for enhanced training and potentially the development of more effective manual disinfection techniques or colored wipes to ensure patient safety and compliance with healthcare hygiene standards. Full article
(This article belongs to the Section Hygiene in Healthcare Facilities)
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12 pages, 5899 KiB  
Article
Common Spontaneous Tumors and Tumor-like Lesions in 70 Pet Rodents and Negative MMTV Detection in Mammary Tumors
by Ya-Mei Chen, Jia-Ling Wu and Wei-Hao Lin
Animals 2024, 14(10), 1469; https://doi.org/10.3390/ani14101469 (registering DOI) - 15 May 2024
Abstract
Compared to the number of studies on the neoplasms of laboratory rodents, fewer studies have focused on spontaneous neoplasms in pet rodents. Notably, the mouse mammary tumor virus (MMTV) is associated with mammary tumors in rodents. In this study, 77 tumors and tumor-like [...] Read more.
Compared to the number of studies on the neoplasms of laboratory rodents, fewer studies have focused on spontaneous neoplasms in pet rodents. Notably, the mouse mammary tumor virus (MMTV) is associated with mammary tumors in rodents. In this study, 77 tumors and tumor-like lesions of biopsy samples were collected from 70 pet rodents, including hamsters (n = 47), guinea pigs (n = 16), unknown species (n = 4), rats (n = 2), and a gerbil. Fifty tumors were collected from 47 hamsters, in which the most common tumors were mammary tumors (13/50), followed by fibrosarcoma (9/50), mast cell tumors (4/50), and squamous cell carcinoma (4/50). The collected subtypes of mammary tumors in hamsters included tubular carcinoma (n = 5), tubular adenoma (n = 4), carcinoma and malignant myoepithelioma (n = 1), simple tubular carcinoma (n = 1), adenosquamous carcinoma (n = 1), and tubulopapillary adenoma (n = 1). In addition, twenty tumors were collected from guinea pigs, in which the most common tumor was lipoma (6/20), followed by adenocarcinoma of the mammary gland (4/20), trichofolliculoma (2/20), and collagenous hamartomas (2/20). In guinea pigs, the subtypes of mammary gland tumors were tubular carcinoma (n = 2), tubular and solid carcinoma (n = 1), and tubulopapillary carcinoma (n = 1). In 20 cases of mammary tumors, MMTV was not detected, implicating no evidence of MMTV infection in mammary oncogenesis in pet rodents in Taiwan. Full article
(This article belongs to the Section Veterinary Clinical Studies)
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18 pages, 1618 KiB  
Article
Temporal and Spatial Influences on Fawn Summer Survival in Pronghorn Populations: Management Implications from Noninvasive Monitoring
by Cole A. Bleke, Eric M. Gese, Juan J. Villalba, Shane B. Roberts and Susannah S. French
Animals 2024, 14(10), 1468; https://doi.org/10.3390/ani14101468 (registering DOI) - 15 May 2024
Abstract
Monitoring vital rates allows managers to estimate trends in growth rates of ungulate populations. However, connecting the influence of nutrition on ungulate demography is challenging. Noninvasive sampling offers a low-cost, low-effort alternative for measuring nutritional indices, allowing for an increased understanding of the [...] Read more.
Monitoring vital rates allows managers to estimate trends in growth rates of ungulate populations. However, connecting the influence of nutrition on ungulate demography is challenging. Noninvasive sampling offers a low-cost, low-effort alternative for measuring nutritional indices, allowing for an increased understanding of the mechanistic relationships between environmental factors, nutrition, and specific population vital rates. We examined the temporal influence of intrinsic and extrinsic factors on pronghorn (Antilocapra americana) fawn recruitment. We collected fresh fecal samples from adult female pronghorn in five subpopulations spanning three sampling periods associated with critical maternal life-history stages (late gestation, early lactation, breeding season) for 2 years to investigate both intra- and interannual influences. Intrinsic factors were fecal glucocorticoid metabolites (FGMs), nutritional indices (fecal nitrogen (FN) and 2,6-diaminopimelic acid (DAPA)), and dietary composition (protein intake of forbs, graminoids, legumes, other, shrubs), while the extrinsic factor was vegetative greenness (normalized difference vegetation index (NDVI)). We found variations in DAPA, protein intake of forbs, variation in forb protein intake, and protein intake of legumes during late gestation positively influenced fawn recruitment. Fecal nitrogen during early lactation showed the strongest positive influence on the recruitment of any measured parameter. Finally, breeding season NDVI and the variation in DAPA values positively influenced the subsequent year’s fawn recruitment. Our longitudinal study enabled us to investigate which parameter was most important to specific periods of fawn development and recruitment. We combined the results across five subpopulations, but interpretation and subsequent management decisions should be made at the subpopulation level such that pronghorn subpopulations with low recruitment can be positively influenced by increasing nitrogen on the landscape available to adult females during the early lactation period. As the use of noninvasive monitoring methods continues to expand, we believe our methodologies and results can be broadly applied to other ungulate monitoring programs. Full article
(This article belongs to the Section Wildlife)
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25 pages, 9166 KiB  
Article
Molecular Mechanism of Phosphorylation-Mediated Impacts on the Conformation Dynamics of GTP-Bound KRAS Probed by GaMD Trajectory-Based Deep Learning
by Jianzhong Chen, Jian Wang, Wanchun Yang, Lu Zhao, Juan Zhao and Guodong Hu
Molecules 2024, 29(10), 2317; https://doi.org/10.3390/molecules29102317 (registering DOI) - 15 May 2024
Abstract
The phosphorylation of different sites produces a significant effect on the conformational dynamics of KRAS. Gaussian accelerated molecular dynamics (GaMD) simulations were combined with deep learning (DL) to explore the molecular mechanism of the phosphorylation-mediated effect on conformational dynamics of the GTP-bound KRAS. [...] Read more.
The phosphorylation of different sites produces a significant effect on the conformational dynamics of KRAS. Gaussian accelerated molecular dynamics (GaMD) simulations were combined with deep learning (DL) to explore the molecular mechanism of the phosphorylation-mediated effect on conformational dynamics of the GTP-bound KRAS. The DL finds that the switch domains are involved in obvious differences in conformation contacts and suggests that the switch domains play a key role in the function of KRAS. The analyses of free energy landscapes (FELs) reveal that the phosphorylation of pY32, pY64, and pY137 leads to more disordered states of the switch domains than the wild-type (WT) KRAS and induces conformational transformations between the closed and open states. The results from principal component analysis (PCA) indicate that principal motions PC1 and PC2 are responsible for the closed and open states of the phosphorylated KRAS. Interaction networks were analyzed and the results verify that the phosphorylation alters interactions of GTP and magnesium ion Mg2+ with the switch domains. It is concluded that the phosphorylation pY32, pY64, and pY137 tune the activity of KRAS through changing conformational dynamics and interactions of the switch domains. We anticipated that this work could provide theoretical aids for deeply understanding the function of KRAS. Full article
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18 pages, 10156 KiB  
Article
Reinforcement of DC Electrified Railways by a Modular Battery Energy Storage System
by Erick Matheus da Silveira Brito, Philippe Ladoux, Joseph Fabre and Benoit Sonier
Electronics 2024, 13(10), 1933; https://doi.org/10.3390/electronics13101933 (registering DOI) - 15 May 2024
Abstract
DC railway electrification was deployed at the beginning of the 20th century in several countries in Europe. Today, this power system is no longer adapted to the demands of increased rail traffic. Due to the relatively low voltage level, the current consumed by [...] Read more.
DC railway electrification was deployed at the beginning of the 20th century in several countries in Europe. Today, this power system is no longer adapted to the demands of increased rail traffic. Due to the relatively low voltage level, the current consumed by the trains reaches several kAs. So, in the worst case, the locomotives cannot operate at their rated power due to the voltage drop along the contact line. Conventional solutions to reduce the voltage drop consist of increasing the cross-section of overhead lines or reducing the length of sectors by installing additional substations. Nevertheless, these solutions are expensive and not always feasible. The implementation of a Modular Battery Energy Storage System (MBESS) can be an alternative solution to reinforce the railway power supply. This paper first presents an MBESS based on elementary blocks associating Full-SiC Isolated DC-DC converter and battery racks. The electrical models of a railway sector and an elementary block are described, and simulations are performed considering real railroad traffic on two sectors of the French National Rail Network, electrified at 1.5 kV. The results show that the installation of an MBESS in the railway sector boosts the locomotive’s voltage while also increasing overall system efficiency. Full article
(This article belongs to the Special Issue Railway Traction Power Supply, 2nd Edition)
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19 pages, 4804 KiB  
Article
Mapping Bovine Tuberculosis in Colombia, 2001–2019
by D. Katterine Bonilla-Aldana, S. Daniela Jiménez-Diaz, Carlos Lozada-Riascos, Kenneth Silva-Cajaleon and Alfonso J. Rodríguez-Morales
Vet. Sci. 2024, 11(5), 220; https://doi.org/10.3390/vetsci11050220 (registering DOI) - 15 May 2024
Abstract
Introduction: Bovine tuberculosis is a zoonotic disease of significant impact, particularly in countries where a pastoral economy is predominant. Despite its importance, few studies have analysed the disease’s behaviour in Colombia, and none have developed maps using geographic information systems (GIS) to characterise [...] Read more.
Introduction: Bovine tuberculosis is a zoonotic disease of significant impact, particularly in countries where a pastoral economy is predominant. Despite its importance, few studies have analysed the disease’s behaviour in Colombia, and none have developed maps using geographic information systems (GIS) to characterise it; as such, we developed this study to describe the temporal–spatial distribution of bovine tuberculosis in Colombia over a period of 19 years. Methods: A retrospective cross-sectional descriptive study, based on reports by the Colombian Agricultural Institute (ICA), surveillance of tuberculosis on cattle farms in Colombia from 2001 to 2019 was carried out. The data were converted into databases using Microsoft Access 365®, and multiple epidemiological maps were generated with the QGIS® version 3.36 software coupled to shape files of all the country’s departments. Results: During the study period, 5273 bovine tuberculosis cases were identified in multiple different departments of Colombia (with a mean of 278 cases/year). Regarding its temporal distribution, the number of cases varied from a maximum of 903 cases (17.12% of the total) in 2015 to a minimum of 0 between 2001 and 2004 and between 2017 and 2019 (between 2005 and 2016, the minimum was 46 cases, 0.87%). Conclusions: GIS are essential for understanding the temporospatial behaviour of zoonotic diseases in Colombia, as is the case for bovine tuberculosis, with its potential implications for the Human and One Health approaches. Full article
(This article belongs to the Special Issue Spotlight on Tuberculosis and Mycoplasmosis in Cattle)
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14 pages, 2979 KiB  
Article
Tumor-Intrinsic Enhancer of Zeste Homolog 2 Controls Immune Cell Infiltration, Tumor Growth, and Lung Metastasis in a Triple-Negative Breast Cancer Model
by Lenore Monterroza, Maria M. Parrilla, Sarah G. Samaranayake, Dormarie E. Rivera-Rodriguez, Sung Bo Yoon, Ramireddy Bommireddy, Justin Hosten, Luisa Cervantes Barragan, Adam Marcus, Brian S. Dobosh, Periasamy Selvaraj and Rabindra Tirouvanziam
Int. J. Mol. Sci. 2024, 25(10), 5392; https://doi.org/10.3390/ijms25105392 (registering DOI) - 15 May 2024
Abstract
Triple-negative breast cancer (TNBC) is an aggressive and highly metastatic type of tumor. TNBC is often enriched in tumor-infiltrating neutrophils (TINs), which support cancer growth in part by counteracting tumor-infiltrating lymphocytes (TILs). Prior studies identified the enhancer of zeste homolog 2 (EZH2) as [...] Read more.
Triple-negative breast cancer (TNBC) is an aggressive and highly metastatic type of tumor. TNBC is often enriched in tumor-infiltrating neutrophils (TINs), which support cancer growth in part by counteracting tumor-infiltrating lymphocytes (TILs). Prior studies identified the enhancer of zeste homolog 2 (EZH2) as a pro-tumor methyltransferase in primary and metastatic TNBCs. We hypothesized that EZH2 inhibition in TNBC cells per se would exert antitumor activity by altering the tumor immune microenvironment. To test this hypothesis, we used CRISPR to generate EZH2 gene knockout (KO) and overexpressing (OE) lines from parent (wild-type—WT) 4T1 cells, an established murine TNBC model, resulting in EZH2 protein KO and OE, respectively. In vitro, EZH2 KO and OE cells showed early, transient changes in replicative capacity and invasiveness, and marked changes in surface marker profile and cytokine/chemokine secretion compared to WT cells. In vivo, EZH2 KO cells showed significantly reduced primary tumor growth and a 10-fold decrease in lung metastasis compared to WT cells, while EZH2 OE cells were unchanged. Compared to WT tumors, TIN:TIL ratios were greatly reduced in EZH2 KO tumors but unchanged in EZH2 OE tumors. Thus, EZH2 is key to 4T1 aggressiveness as its tumor-intrinsic knockout alters their in vitro secretome and in vivo primary tumor growth, TIN/TIL poise, and metastasis. Full article
(This article belongs to the Special Issue Advances and Mechanisms in Breast Cancer)
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5 pages, 181 KiB  
Book Review
Book Review: Powdyel, T.S. My Green School: An Outline; 2020; ISBN-13: 979-8677417979
by Anh-Tuan Hoang and Tzu-Bin Lin
Sustainability 2024, 16(10), 4131; https://doi.org/10.3390/su16104131 (registering DOI) - 15 May 2024
Abstract
The Green School—An outline reflects on the author’s vision of a holistic approach to the education system as a leading arrow for human beings and social prosperity. It is a needed book, an education of choice, conviction, and passion by Thakur S. Powdyel, [...] Read more.
The Green School—An outline reflects on the author’s vision of a holistic approach to the education system as a leading arrow for human beings and social prosperity. It is a needed book, an education of choice, conviction, and passion by Thakur S. Powdyel, former Minister of Education, Royal Government of Bhutan a long with several studies in terms of reflections on Gross National Happiness in the education context and globalization, and context of development. Thus, Powdyel’s Green School conducts all the fundamentals as well as many other crucial aspects related to greenery concepts in the entire school as a whole-green school approach for future education. Full article
26 pages, 8045 KiB  
Article
Improving Wheat Leaf Nitrogen Concentration (LNC) Estimation across Multiple Growth Stages Using Feature Combination Indices (FCIs) from UAV Multispectral Imagery
by Xiangxiang Su, Ying Nian, Hu Yue, Yongji Zhu, Jun Li, Weiqiang Wang, Yali Sheng, Qiang Ma, Jikai Liu, Wenhui Wang and Xinwei Li
Agronomy 2024, 14(5), 1052; https://doi.org/10.3390/agronomy14051052 (registering DOI) - 15 May 2024
Abstract
Leaf nitrogen concentration (LNC) is a primary indicator of crop nitrogen status, closely related to the growth and development dynamics of crops. Accurate and efficient monitoring of LNC is significant for precision field crop management and enhancing crop productivity. However, the biochemical properties [...] Read more.
Leaf nitrogen concentration (LNC) is a primary indicator of crop nitrogen status, closely related to the growth and development dynamics of crops. Accurate and efficient monitoring of LNC is significant for precision field crop management and enhancing crop productivity. However, the biochemical properties and canopy structure of wheat change across different growth stages, leading to variations in spectral responses that significantly impact the estimation of wheat LNC. This study aims to investigate the construction of feature combination indices (FCIs) sensitive to LNC across multiple wheat growth stages, using remote sensing data to develop an LNC estimation model that is suitable for multiple growth stages. The research employs UAV multispectral remote sensing technology to acquire canopy imagery of wheat during the early (Jointing stage and Booting stage) and late (Early filling and Late filling stages) in 2021 and 2022, extracting spectral band reflectance and texture metrics. Initially, twelve sensitive spectral feature combination indices (SFCIs) were constructed using spectral band information. Subsequently, sensitive texture feature combination indices (TFCIs) were created using texture metrics as an alternative to spectral bands. Machine learning algorithms, including partial least squares regression (PLSR), random forest regression (RFR), support vector regression (SVR), and Gaussian process regression (GPR), were used to integrate spectral and texture information, enhancing the estimation performance of wheat LNC across growth stages. Results show that the combination of Red, Red edge, and Near-infrared bands, along with texture metrics such as Mean, Correlation, Contrast, and Dissimilarity, has significant potential for LNC estimation. The constructed SFCIs and TFCIs both enhanced the responsiveness to LNC across multiple growth stages. Additionally, a sensitive index, the Modified Vegetation Index (MVI), demonstrated significant improvement over NDVI, correcting the over-saturation concerns of NDVI in time-series analysis and displaying outstanding potential for LNC estimation. Spectral information outperforms texture information in estimation capability, and their integration, particularly with SVR, achieves the highest precision (coefficient of determination (R2) = 0.786, root mean square error (RMSE) = 0.589%, and relative prediction deviation (RPD) = 2.162). In conclusion, the sensitive FCIs developed in this study improve LNC estimation performance across multiple growth stages, enabling precise monitoring of wheat LNC. This research provides insights and technical support for the construction of sensitive indices and the precise management of nitrogen nutrition status in field crops. Full article
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19 pages, 4565 KiB  
Article
Novel Acetamide-Based HO-1 Inhibitor Counteracts Glioblastoma Progression by Interfering with the Hypoxic–Angiogenic Pathway
by Agata Grazia D’Amico, Grazia Maugeri, Luca Vanella, Valeria Consoli, Valeria Sorrenti, Francesca Bruno, Concetta Federico, Antonino Nicolò Fallica, Valeria Pittalà and Velia D’Agata
Int. J. Mol. Sci. 2024, 25(10), 5389; https://doi.org/10.3390/ijms25105389 (registering DOI) - 15 May 2024
Abstract
Glioblastoma multiforme (GBM) represents the deadliest tumor among brain cancers. It is a solid tumor characterized by uncontrolled cell proliferation generating the hypoxic niches in the cancer core. By inducing the transcription of hypoxic inducible factor (HIF), hypoxia triggers many signaling cascades responsible [...] Read more.
Glioblastoma multiforme (GBM) represents the deadliest tumor among brain cancers. It is a solid tumor characterized by uncontrolled cell proliferation generating the hypoxic niches in the cancer core. By inducing the transcription of hypoxic inducible factor (HIF), hypoxia triggers many signaling cascades responsible for cancer progression and aggressiveness, including enhanced expression of vascular endothelial growth factor (VEGF) or antioxidant enzymes, such as heme oxygenase-1 (HO-1). The present work aimed to investigate the link between HO-1 expression and the hypoxic microenvironment of GBM by culturing two human glioblastoma cell lines (U87MG and A172) in the presence of a hypoxic mimetic agent, deferoxamine (DFX). By targeting hypoxia-induced HO-1, we have tested the effect of a novel acetamide-based HO-1 inhibitor (VP18/58) on GBM progression. Results have demonstrated that hypoxic conditions induced upregulation and nuclear expression of HO-1 in a cell-dependent manner related to malignant phenotype. Moreover, our data demonstrated that the HO-1 inhibitor counteracted GBM progression by modulating the HIFα/HO-1/VEGF signaling cascade in cancer cells bearing more malignant phenotypes. Full article
(This article belongs to the Special Issue Molecular Progression of Genome-Related Diseases)
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17 pages, 4692 KiB  
Article
Genomic and Transcriptome Analysis Reveals the Biosynthesis Network of Cordycepin in Cordyceps militaris
by Linshan Chai, Jianmei Li, Lingling Guo, Shuyu Zhang, Fei Chen, Wanqin Zhu and Yu Li
Genes 2024, 15(5), 626; https://doi.org/10.3390/genes15050626 (registering DOI) - 15 May 2024
Abstract
Cordycepin is the primary active compound of Cordyceps militaris. However, the definitive genetic mechanism governing cordycepin synthesis in fruiting body growth and development remains elusive, necessitating further investigation. This study consists of 64 C. militaris strains collected from northeast China. The high-yielding [...] Read more.
Cordycepin is the primary active compound of Cordyceps militaris. However, the definitive genetic mechanism governing cordycepin synthesis in fruiting body growth and development remains elusive, necessitating further investigation. This study consists of 64 C. militaris strains collected from northeast China. The high-yielding cordycepin strain CMS19 was selected for the analysis of cordycepin production and the genetic basis of cordycepin anabolism. First, the whole-genome sequencing of CMS19 yielded a final size of 30.96 Mb with 8 contigs and 9781 protein-coding genes. The genome component revealed the presence of four additional secondary metabolite gene clusters compared with other published genomes, suggesting the potential for the production of new natural products. The analyses of evolutionary and genetic differentiation revealed a close relationship between C. militaris and Beauveria bassiana. The population of strains distributed in northeast China exhibited the significant genetic variation. Finally, functional genes associated with cordycepin synthesis were identified using a combination of genomic and transcriptomic analyses. A large number of functional genes associated with energy and purine metabolism were significantly enriched, facilitating the reconstruction of a hypothetical cordycepin metabolic pathway. Therefore, our speculation of the cordycepin metabolism pathway involved 24 genes initiating from the glycolysis and pentose phosphate pathways, progressing through purine metabolism, and culminating in the core region of cordycepin synthesis. These findings could offer fundamental support for scientific utilizations of C. militaris germplasm resources and standardized cultivation for cordycepin production. Full article
(This article belongs to the Section Plant Genetics and Genomics)
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20 pages, 739 KiB  
Review
Research Progress in the Separation of Chemical Components from Essential Oils by High-Speed Countercurrent Chromatography
by Linhong He, Zihao Zhong, Lijuan Zhang and Xi Bai
Separations 2024, 11(5), 152; https://doi.org/10.3390/separations11050152 (registering DOI) - 15 May 2024
Abstract
Essential oils (EOs) are vital secondary metabolites in plants. They have garnered substantial attention owing to their distinct flavors and desirable attributes, including potent antioxidant, antibacterial, and antitumor properties. Nevertheless, the active constituents of EOs exhibit intricate chemical structures, and conventional separation techniques [...] Read more.
Essential oils (EOs) are vital secondary metabolites in plants. They have garnered substantial attention owing to their distinct flavors and desirable attributes, including potent antioxidant, antibacterial, and antitumor properties. Nevertheless, the active constituents of EOs exhibit intricate chemical structures, and conventional separation techniques are inadequate for purifying the individual chemical components from EOs. High-speed countercurrent chromatography, based on the principles of a hydrodynamic equilibrium system, has emerged as a liquid–liquid chromatographic separation method renowned for its ability to handle substantial single injection volumes and the absence of irreversible adsorption. Consequently, in recent years, this technique has been widely employed in the isolation and refinement of natural products. In this review, a comprehensive analysis is conducted, contrasting the merits and demerits of high-speed countercurrent chromatography with conventional separation methods. The solvent systems, elution modes, commonly employed detectors, and practical applications are reviewed in the context of high-speed countercurrent chromatography for essential oil separation and purification. Furthermore, this review offers a glimpse into the potential prospects of applying this technique, with the intention of serving as a valuable reference for the use of high-speed countercurrent chromatography in the purification of EOs. Full article
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24 pages, 4955 KiB  
Article
Phenomenology of the Composition of PM2.5 at an Urban Site in Northern France
by Yamina Allouche, Marc Fadel, Amélie Ferté, Anthony Verdin, Frédéric Ledoux and Dominique Courcot
Atmosphere 2024, 15(5), 603; https://doi.org/10.3390/atmos15050603 (registering DOI) - 15 May 2024
Abstract
In this work, PM2.5 was sampled at Dunkerque, a medium-sized city located in northern France. The mean concentration of PM2.5 during the sampling period was 12.6 ± 9.5 μg·m−3. Samples were analyzed for elemental and organic carbon (EC/OC), water-soluble [...] Read more.
In this work, PM2.5 was sampled at Dunkerque, a medium-sized city located in northern France. The mean concentration of PM2.5 during the sampling period was 12.6 ± 9.5 μg·m−3. Samples were analyzed for elemental and organic carbon (EC/OC), water-soluble organic carbon (WSOC), humic-like substances (HULIS-C), water-soluble inorganic ions, and major and trace elements. The origin and the variations of species concentrations were examined using elemental enrichment factors, bivariate polar plot representations, and diagnostic concentration ratios. Secondary inorganic ions were the most abundant species (36% of PM2.5), followed by OC (12.5% of PM2.5). Secondary organic carbon (SOC) concentrations were estimated to account for 52% of OC. A good correlation between SOC and WSOC indicated that secondary formation processes significantly contribute to the WSOC concentrations. HULIS-C also represents almost 50% of WSOC. The determination of diagnostic ratios revealed the influence of anthropogenic emission sources such as integrated steelworks and fuel oil combustion. The clustering of 72 h air masses backward trajectories data evidenced that higher concentrations of PM2.5, OC, and secondary inorganic aerosols were recorded when air masses came from north-eastern Europe and the French continental sector, showing the considerable impact of long-range transport on the air quality in northern France. Full article
(This article belongs to the Special Issue Characteristics and Source Apportionment of Urban Air Pollution)
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15 pages, 9807 KiB  
Article
Investigation of the Tribological Properties of Hybrid Additive-Modified Water-Based Lubricating Fluid
by Raimondas Kreivaitis, Jolanta Treinytė, Artūras Kupčinskas, Milda Gumbytė and Eglė Sendžikienė
Lubricants 2024, 12(5), 178; https://doi.org/10.3390/lubricants12050178 (registering DOI) - 15 May 2024
Abstract
Water-based lubricating fluids (WBLFs), known for their significant environmental benefits, are the focus of this study. The properties of WBLFs directly influence lubricated mechanisms’ longevity and operating efficiency. WBLFs are enhanced using additives, which must improve their properties and, at the same time, [...] Read more.
Water-based lubricating fluids (WBLFs), known for their significant environmental benefits, are the focus of this study. The properties of WBLFs directly influence lubricated mechanisms’ longevity and operating efficiency. WBLFs are enhanced using additives, which must improve their properties and, at the same time, remain environmentally friendly. This study combines bis(2-hydroxyethyl) ammonium erucate protic ionic liquid and titanium oxide nanoparticles to formulate the hybrid additive. The lubricity was investigated using Alumina/Bearing steel and WC/Bearing steel friction pairs in a reciprocating ball-on-plate tribo-tester. The results show that protic ionic liquid can significantly improve lubricity and the corrosion-preventing ability of the base fluid. Applying a hybrid additive further improved the wear reduction ability in the WC/Bearing steel friction pair. However, the wear reduction ability was diminished when a hybrid additive was used to lubricate the Alumina/Bearing steel friction pair. The proposed lubricity improvement mechanism is based on forming an adsorption layer of ionic liquid molecules and rolling and tribo-sintering titanium oxide nanoparticles. Full article
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13 pages, 6417 KiB  
Article
Preparation and Application of High Internal Phase Pickering Emulsion Gels Stabilized by Starch Nanocrystal/Tannic Acid Complex Particles
by Haoran Jin, Chen Li, Yajuan Sun, Bingtian Zhao and Yunxing Li
Gels 2024, 10(5), 335; https://doi.org/10.3390/gels10050335 (registering DOI) - 15 May 2024
Abstract
Herein, the starch nanocrystal/tannic acid (ST) complex particles, which were prepared based on the hydrogen bond between starch nanocrystal (SNC) and tannic acid (TA), were successfully used to stabilize the HIPPE gels. The optimal TA concentration of the ST complex particles resulted in [...] Read more.
Herein, the starch nanocrystal/tannic acid (ST) complex particles, which were prepared based on the hydrogen bond between starch nanocrystal (SNC) and tannic acid (TA), were successfully used to stabilize the HIPPE gels. The optimal TA concentration of the ST complex particles resulted in better water dispersibility, surface wettability, and interfacial activity as compared to SNC. The hydrogen bond responsible for the formation of ST complex particles and subsequent stable emulsions was demonstrated by varying the pH and ionic strength of the aqueous phase. Notably, the HIPPE gels stabilized via the ST complex particles can maintain long-term stability for up to three months. The HIPPEs stabilized via the ST complex particles all displayed gel-like features and had smaller droplets and denser droplet networks than the SNC-stabilized HIPPEs. The rheological behavior of HIPPE gels stabilized via the ST complex particles can be readily changed by tuning the mass ratio of SNC and TA as well as pH. Finally, the prepared HIPPE gels used to effectively protect encapsulated β-carotene against high temperatures and ultraviolet radiation and its controllable release at room temperature were demonstrated. It is anticipated that the aforementioned findings will provide new perspectives on the preparation of Pickering emulsion for delivery systems. Full article
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19 pages, 4186 KiB  
Article
Experimental Study on Evaporation and Micro-Explosion Characteristics of Ethanol and Diesel Blended Droplets
by Yixuan Zhang, Kesheng Meng, Lin Bao, Qizhao Lin and Svitlana Pavlova
Atmosphere 2024, 15(5), 604; https://doi.org/10.3390/atmos15050604 (registering DOI) - 15 May 2024
Abstract
In this study, the constant temperature control system of a heating plate was established, ethanol–diesel fuel with different proportions was prepared, and a series of experiments were carried out. The experimental system was used to observe, summarize, and analyze four evaporation and crushing [...] Read more.
In this study, the constant temperature control system of a heating plate was established, ethanol–diesel fuel with different proportions was prepared, and a series of experiments were carried out. The experimental system was used to observe, summarize, and analyze four evaporation and crushing modes of mixed droplets, which were explosion, liquid filament stretching, exocytosis, and ejection mode. The evaporation process of four kinds of mixed droplets in their life cycle was analyzed by normalizing the diameter square. It was proposed that the evaporation process of droplets could be divided into the following three stages: a heating stage, a fluctuating evaporation stage, and an equilibrium evaporation stage. It was also pointed out that the expansion, ejection, and micro-explosion of droplets were the causes of fluctuating evaporation. The concept of expansion and crushing intensity was put forward and the expansion and crushing intensity of ethanol/diesel mixed droplets with different proportions were calculated. The reasons why expansion and crushing intensity first increased and decreased with the increase in ethanol blending ratio were analyzed. Finally, the time proportion of ethanol–diesel mixed droplets in each evaporation stage was calculated, which explained that the time proportion of the instantaneous heating stage showed a parabolic law with the increase in ethanol content. Full article
(This article belongs to the Special Issue Engine Emissions: Assessment and Control)
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11 pages, 2004 KiB  
Article
Nobiletin Stimulates Adrenal Hormones and Modulates the Circadian Clock in Mice
by Conn Ryan, Yu Tahara, Atsushi Haraguchi, Yuanyuan Lu and Shigenobu Shibata
Nutrients 2024, 16(10), 1491; https://doi.org/10.3390/nu16101491 (registering DOI) - 15 May 2024
Abstract
Polymethoxyflavonoids, such as nobiletin (abundant in Citrus depressa), have been reported to have antioxidant, anti-inflammatory, anticancer, and anti-dementia effects, and are also a circadian clock modulator through retinoic acid receptor-related orphan receptor (ROR) α/γ. However, the optimal timing of nobiletin intake has not [...] Read more.
Polymethoxyflavonoids, such as nobiletin (abundant in Citrus depressa), have been reported to have antioxidant, anti-inflammatory, anticancer, and anti-dementia effects, and are also a circadian clock modulator through retinoic acid receptor-related orphan receptor (ROR) α/γ. However, the optimal timing of nobiletin intake has not yet been determined. Here, we explored the time-dependent treatment effects of nobiletin and a possible novel mechanistic idea for nobiletin-induced circadian clock regulation in mice. In vivo imaging showed that the PER2::LUC rhythm in the peripheral organs was altered in accordance with the timing of nobiletin administration (100 mg/kg). Administration at ZT4 (middle of the light period) caused an advance in the peripheral clock, whereas administration at ZT16 (middle of the dark period) caused an increase in amplitude. In addition, the intraperitoneal injection of nobiletin significantly and potently stimulated corticosterone and adrenaline secretion and caused an increase in Per1 expression in the peripheral tissues. Nobiletin inhibited phosphodiesterase (PDE) 4A1A, 4B1, and 10A2. Nobiletin or rolipram (PDE4 inhibitor) injection, but not SR1078 (RORα/γ agonist), caused acute Per1 expression in the peripheral tissues. Thus, the present study demonstrated a novel function of nobiletin and the regulation of the peripheral circadian clock. Full article
(This article belongs to the Section Micronutrients and Human Health)
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19 pages, 2300 KiB  
Review
The Stria Vascularis: Renewed Attention on a Key Player in Age-Related Hearing Loss
by Sonny Bovee, Georg M. Klump, Christine Köppl and Sonja J. Pyott
Int. J. Mol. Sci. 2024, 25(10), 5391; https://doi.org/10.3390/ijms25105391 (registering DOI) - 15 May 2024
Abstract
Age-related hearing loss (HL), or presbycusis, is a complex and heterogeneous condition, affecting a significant portion of older adults and involving various interacting mechanisms. Metabolic presbycusis, a type of age-related HL, is characterized by the dysfunction of the stria vascularis, which is crucial [...] Read more.
Age-related hearing loss (HL), or presbycusis, is a complex and heterogeneous condition, affecting a significant portion of older adults and involving various interacting mechanisms. Metabolic presbycusis, a type of age-related HL, is characterized by the dysfunction of the stria vascularis, which is crucial for maintaining the endocochlear potential necessary for hearing. Although attention on metabolic presbycusis has waned in recent years, research continues to identify strial pathology as a key factor in age-related HL. This narrative review integrates past and recent research, bridging findings from animal models and human studies, to examine the contributions of the stria vascularis to age-related HL. It provides a brief overview of the structure and function of the stria vascularis and then examines mechanisms contributing to age-related strial dysfunction, including altered ion transport, changes in pigmentation, inflammatory responses, and vascular atrophy. Importantly, this review outlines the contribution of metabolic mechanisms to age-related HL, highlighting areas for future research. It emphasizes the complex interdependence of metabolic and sensorineural mechanisms in the pathology of age-related HL and highlights the importance of animal models in understanding the underlying mechanisms. The comprehensive and mechanistic investigation of all factors contributing to age-related HL, including cochlear metabolic dysfunction, remains crucial to identifying the underlying mechanisms and developing personalized, protective, and restorative treatments. Full article
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12 pages, 2987 KiB  
Article
Hourglass Magnetic Field of a Protostellar System
by Shantanu Basu, Xiyuan Li and Gianfranco Bino
Universe 2024, 10(5), 218; https://doi.org/10.3390/universe10050218 (registering DOI) - 15 May 2024
Abstract
An hourglass-shaped magnetic field pattern arises naturally from the gravitational collapse of a star-forming gas cloud. Most studies have focused on the prestellar collapse phase, when the structure has a smooth and monotonic radial profile. However, most observations target dense clouds that already [...] Read more.
An hourglass-shaped magnetic field pattern arises naturally from the gravitational collapse of a star-forming gas cloud. Most studies have focused on the prestellar collapse phase, when the structure has a smooth and monotonic radial profile. However, most observations target dense clouds that already contain a central protostar, and possibly a circumstellar disk. We utilize an analytic treatment of the magnetic field along with insights gained from simulations to develop a more realistic magnetic field model for the protostellar phase. Key elements of the model are a strong radial magnetic field in the region of rapid collapse, an off-center peak in the magnetic field strength (a consequence of magnetic field dissipation in the circumstellar disk), and a strong toroidal field that is generated in the region of rapid collapse and outflow generation. A model with a highly pinched and twisted magnetic field pattern in the inner collapse zone facilitates the interpretation of magnetic field patterns observed in protostellar clouds. Full article
(This article belongs to the Special Issue Advances in Star Formation in the Milky Way)
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