The 2023 MDPI Annual Report has
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22 pages, 4275 KiB  
Article
Study of Microstructure, Texture, and Cooking Qualities of Reformulated Whole Wheat Flour Pasta by Substituting Water with Stearic Acid–Candelilla Wax–Groundnut Oil Oleogel
by Diksha Chaturvedi, Somali Dhal, Deblu Sahu, Maciej Jarzębski, Arfat Anis, Doman Kim and Kunal Pal
ChemEngineering 2024, 8(3), 51; https://doi.org/10.3390/chemengineering8030051 (registering DOI) - 04 May 2024
Abstract
Oleogels, which are traditionally utilized to reduce saturated and trans fats in bakery foods, have recently shown promising applications in non-bakery foods, particularly in the enhancement of their food texture and cooking qualities. This study investigates the impact of incorporating stearic acid-containing candelilla [...] Read more.
Oleogels, which are traditionally utilized to reduce saturated and trans fats in bakery foods, have recently shown promising applications in non-bakery foods, particularly in the enhancement of their food texture and cooking qualities. This study investigates the impact of incorporating stearic acid-containing candelilla wax–groundnut oil oleogel in various proportions on the production of whole wheat pasta. Five different pasta samples were prepared by replacing water with oleogels in varying concentrations (2.5%, 5%, 10%, and 15%), and their physicochemical attributes were evaluated using a range of analytical methods for both cooked and uncooked pasta (like microscopy, colorimetry, dimensional analysis, texture, cooking qualities, moisture content, and FTIR). Significant differences in width, thickness, and color properties were observed between the control sample (0% oleogel) and those containing oleogel, with notable variations in surface texture and color intensities, particularly with the higher oleogel content (p < 0.05). Cooked pasta exhibited lower L* values and higher a* values than uncooked pasta. Stereo zoom microscope and field emission scanning electron microscope (FESEM) micrographs demonstrated a change in the pasta surface topology and microstructures. Dark spots on the pasta with greater oleogel concentrations (samples with 10% and 15% oleogel replacement) suggest the formation of starch–lipid complexes. Cooking induced pore formation, which was more pronounced when the oleogel content was increased, impacted the water absorption capacity, swelling index, and moisture content. The cooked samples exhibited higher moisture content and improved polymer network stability compared to the uncooked ones, indicating the potential of oleogel incorporation to modulate pasta properties in a concentration-dependent manner. These findings underscore the versatility of oleogels when their applications are diversified in non-bakery foods to enhance food texture and quality. Full article
22 pages, 412 KiB  
Article
Sustainability under Active Inference
by Mahault Albarracin, Maxwell Ramstead, Riddhi J. Pitliya, Ines Hipolito, Lancelot Da Costa, Maria Raffa, Axel Constant and Sarah Grace Manski
Systems 2024, 12(5), 163; https://doi.org/10.3390/systems12050163 (registering DOI) - 04 May 2024
Abstract
In this paper, we explore the known connection among sustainability, resilience, and well-being within the framework of active inference. Initially, we revisit how the notions of well-being and resilience intersect within active inference before defining sustainability. We adopt a holistic concept of sustainability [...] Read more.
In this paper, we explore the known connection among sustainability, resilience, and well-being within the framework of active inference. Initially, we revisit how the notions of well-being and resilience intersect within active inference before defining sustainability. We adopt a holistic concept of sustainability denoting the enduring capacity to meet needs over time without depleting crucial resources. It extends beyond material wealth to encompass community networks, labor, and knowledge. Using the free energy principle, we can emphasize the role of fostering resource renewal, harmonious system–entity exchanges, and practices that encourage self-organization and resilience as pathways to achieving sustainability both as an agent and as a part of a collective. We start by connecting active inference with well-being, building on exsiting work. We then attempt to link resilience with sustainability, asserting that resilience alone is insufficient for sustainable outcomes. While crucial for absorbing shocks and stresses, resilience must be intrinsically linked with sustainability to ensure that adaptive capacities do not merely perpetuate existing vulnerabilities. Rather, it should facilitate transformative processes that address the root causes of unsustainability. Sustainability, therefore, must manifest across extended timescales and all system strata, from individual components to the broader system, to uphold ecological integrity, economic stability, and social well-being. We explain how sustainability manifests at the level of an agent and then at the level of collectives and systems. To model and quantify the interdependencies between resources and their impact on overall system sustainability, we introduce the application of network theory and dynamical systems theory. We emphasize the optimization of precision or learning rates through the active inference framework, advocating for an approach that fosters the elastic and plastic resilience necessary for long-term sustainability and abundance. Full article
19 pages, 1894 KiB  
Article
Digital Transformation, Gender Discrimination, and Female Employment
by Rendao Ye and Xinya Cai
Systems 2024, 12(5), 162; https://doi.org/10.3390/systems12050162 (registering DOI) - 04 May 2024
Abstract
With the demographic dividend disappearing, the key to achieving high-quality development in China is to promote full employment of the workforce. Women are a significant group in the job market, but they frequently face greater pressure and higher employment thresholds. Ensuring high-quality employment [...] Read more.
With the demographic dividend disappearing, the key to achieving high-quality development in China is to promote full employment of the workforce. Women are a significant group in the job market, but they frequently face greater pressure and higher employment thresholds. Ensuring high-quality employment for women will be one of the most important tasks in the future. Based on the China Family Panel Studies data, this paper uses two-way fixed effects models, causal stepwise regression analysis, and structural equation models to study the impact of digital transformation of households on female employment and how it works. The empirical results show that digital transformation of households significantly promotes female employment. For low-security employment and high-security employment, the promotion effect of digital transformation is significant. Further mechanism analysis shows that digital transformation of households mainly increases women’s human capital, improves their search for information, and stimulates improvements in social skills, thus effectively eliminating employment-related gender discrimination and ultimately promoting women’s employment. This paper can provide a significant reference for alleviating female employment pressure, promoting full employment, and achieving high-quality development in the context of digital transformation. Full article
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18 pages, 1234 KiB  
Article
Research on Economic Load Dispatch Problem of Microgrid Based on an Improved Pelican Optimization Algorithm
by Yi Zhang and Haoxue Li
Biomimetics 2024, 9(5), 277; https://doi.org/10.3390/biomimetics9050277 (registering DOI) - 04 May 2024
Abstract
This paper presents an improved pelican optimization algorithm (IPOA) to solve the economic load dispatch problem. The vertical crossover operator in the crisscross optimization algorithm is integrated to expand the diversity of the population in the local search phase. The optimal individual is [...] Read more.
This paper presents an improved pelican optimization algorithm (IPOA) to solve the economic load dispatch problem. The vertical crossover operator in the crisscross optimization algorithm is integrated to expand the diversity of the population in the local search phase. The optimal individual is also introduced to enhance its ability to guide the whole population and add disturbance factors to enhance its ability to jump out of the local optimal. The dimensional variation strategy is adopted to improve the optimal individual and speed up the algorithm’s convergence. The results of the IPOA showed that coal consumption was reduced by 0.0292%, 2.7273%, and 3.6739%, respectively, when tested on 10, 40, and 80-dimensional thermal power plant units compared to POA. The IPOA can significantly reduce the fuel cost of power plants. Full article
(This article belongs to the Special Issue Biomimicry for Optimization, Control, and Automation: 2nd Edition)
12 pages, 3256 KiB  
Article
Miniaturizing Hyperspectral Lidar System Employing Integrated Optical Filters
by Haibin Sun, Yicheng Wang, Zhipei Sun, Shaowei Wang, Shengli Sun, Jianxin Jia, Changhui Jiang, Peilun Hu, Haima Yang, Xing Yang, Mika Karjalnen, Juha Hyyppä and Yuwei Chen
Remote Sens. 2024, 16(9), 1642; https://doi.org/10.3390/rs16091642 (registering DOI) - 04 May 2024
Abstract
Hyperspectral LiDAR (HSL) has been utilized as an efficacious technique in object classification and recognition based on its unique capability to obtain ranges and spectra synchronously. Different kinds of HSL prototypes with varied structures have been promoted and measured its performance. However, almost [...] Read more.
Hyperspectral LiDAR (HSL) has been utilized as an efficacious technique in object classification and recognition based on its unique capability to obtain ranges and spectra synchronously. Different kinds of HSL prototypes with varied structures have been promoted and measured its performance. However, almost all of these HSL prototypes employ complex and large spectroscopic devices, such as an Acousto-Optic Tunable Filter and Liquid-Crystal Tunable Filter, which makes this HSL system bulky and expensive, and then hinders its extensive application in many fields. In this paper, a smart and smaller spectroscopic component, an intergraded optical filter (IOF), is promoted to miniaturize these HSL systems. The system calibration, range precision, and spectral profile experiments were carried out to test the HSL prototype. Although the IOF employed here only covered a wavelength range of 699–758 nm with a six-channel passband and showed a transmittance of less than 50%, the HSL prototype showed excellent performance in ranging and spectral profile collecting. The spectral profiles collected are well in accordance with those acquired based on the AOTF. The spectral profiles of the fruits, vegetables, plants, and ore samples collected by the HSL based on an IOF can effectively reveal the status of the plants, the component materials, and ore species. Finally, we also showed the integrated design of the HSL based on a three-dimensional IOF and combined with a detector. The performance and designs of this HSL system based on an IOF show great potential for miniaturizing in some specific applications. Full article
(This article belongs to the Special Issue Remote Sensing and Lidar Data for Forest Monitoring)
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13 pages, 4390 KiB  
Article
Genetic Evidence for the Presence of Wild-Caught Sturgeons in Commercial Markets in Georgia
by Tamar Beridze, Shannon L. White, David C. Kazyak, Levan Ninua, Dewayne Fox, Arun Sethuraman, Tamari Edisherashvili, Bianca Roberts, Mikheil Potskhishvili, Michelle Klailova and Cort Anderson
Diversity 2024, 16(5), 274; https://doi.org/10.3390/d16050274 (registering DOI) - 04 May 2024
Abstract
Sturgeons (Family: Acipenseridae) are among the most endangered taxa worldwide. Significant resources have been invested into the conservation of global sturgeon populations, including the development of commercial aquaculture programs. These programs are intended to improve conservation outcomes by reducing the harvest of wild [...] Read more.
Sturgeons (Family: Acipenseridae) are among the most endangered taxa worldwide. Significant resources have been invested into the conservation of global sturgeon populations, including the development of commercial aquaculture programs. These programs are intended to improve conservation outcomes by reducing the harvest of wild populations while still meeting commercial demand for sturgeon products. However, there is growing concern that commercial aquaculture programs may contribute to wild population declines through continued, illegal harvest and the escape and/or release of captive individuals into wild environments. These concerns may be particularly acute in the country of Georgia which, despite its small territory and altered landscape, is a globally significant hotspot for sturgeon diversity. In order to understand the potential threat of captive culture on wild sturgeon populations in Georgia, we used mitochondrial DNA sequencing and microsatellite analyses to identify the species and origin of sturgeons encountered in commercial settings. Microsatellite analyses showed significant differentiation between wild and commercial Russian sturgeon populations and highlighted the potential for wild-caught individuals to be present in coastal markets in Georgia. The analyses of mitochondrial haplotypes also suggested that commercial markets may contain sturgeon species that are not native to the region. Overall, our results suggest that wild sturgeon populations may still be exploited to support captive aquaculture programs and commercial sales. Full article
(This article belongs to the Topic Ecology, Management and Conservation of Vertebrates)
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16 pages, 2025 KiB  
Article
A G-Modified Helmholtz Equation with New Expansions for the Earth’s Disturbing Gravitational Potential, Its Functionals and the Study of Isogravitational Surfaces
by Gerassimos Manoussakis
AppliedMath 2024, 4(2), 580-595; https://doi.org/10.3390/appliedmath4020032 (registering DOI) - 04 May 2024
Abstract
The G-modified Helmholtz equation is a partial differential equation that enables us to express gravity intensity g as a series of spherical harmonics having radial distance r in irrational powers. The Laplace equation in three-dimensional space (in Cartesian coordinates, is the sum of [...] Read more.
The G-modified Helmholtz equation is a partial differential equation that enables us to express gravity intensity g as a series of spherical harmonics having radial distance r in irrational powers. The Laplace equation in three-dimensional space (in Cartesian coordinates, is the sum of the second-order partial derivatives of the unknown quantity equal to zero) is used to express the Earth’s gravity potential (disturbing and normal potential) in order to represent other useful quantities—which are also known as functionals of the disturbing potential—such as gravity disturbance, gravity anomaly, and geoid undulation as a series of spherical harmonics. We demonstrate that by using the G-modified Helmholtz equation, not only gravity intensity but also disturbing potential and its functionals can be expressed as a series of spherical harmonics. Having gravity intensity represented as a series of spherical harmonics allows us to create new Global Gravity Models. Furthermore, a more detailed examination of the Earth’s isogravitational surfaces is conducted. Finally, we tabulate our results, which makes it clear that new Global Gravity Models for gravity intensity g will be very useful for many geophysical and geodetic applications. Full article
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12 pages, 962 KiB  
Article
Assessing the Conservation Value of Artificial and Natural Reefs via Ichthyoplankton Spatio-Temporal Dynamics
by Carlos Adrián Sánchez-Caballero, José Manuel Borges-Souza, Ricardo Javier Saldierna-Martínez and Avigdor Abelson
Fishes 2024, 9(5), 166; https://doi.org/10.3390/fishes9050166 (registering DOI) - 04 May 2024
Abstract
The distribution of fish eggs and larvae (ichthyoplankton) reflects spawning and nursery areas as well as dispersal routes. This study’s goal is to demonstrate how the identification of ichthyoplankton species and stages and their spatial distribution among natural reefs (NRs) and artificial reefs [...] Read more.
The distribution of fish eggs and larvae (ichthyoplankton) reflects spawning and nursery areas as well as dispersal routes. This study’s goal is to demonstrate how the identification of ichthyoplankton species and stages and their spatial distribution among natural reefs (NRs) and artificial reefs (ARs) may serve as decision-making tools in conservation and fishery management. Natural reefs exhibited an eight-times higher abundance of eggs, as well as the highest abundance of larvae in the yolk-sac and preflexion phases. In contrast, ARs had the highest abundance of larvae in the flexion and postflexion phases. Natural reefs may serve as breeding grounds for Scaridae, Labridae, and Mugilidae; whereas, ARs may serve as breeding sites for Lutjanidae, Synodontidae, Carangidae, Fistularidae, and Haemulidae. Our study revealed differences between ARs and NRs, which demonstrate the potential of artificial reefs to expand the supply and settlement options of reef fishes and consequently can lead to increased fish production with potential benefits to adjacent fishing areas through connectivity. Thus, ARs as no-take sites can be effective tools for both fishery management and biodiversity conservation. The findings highlight the potential use of ichthyoplankton tools and the importance of considering both types of reefs in marine conservation and management efforts. Full article
(This article belongs to the Special Issue Biology and Ecology of Coral Reef Fishes)
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19 pages, 2433 KiB  
Review
Challenges in Echocardiography for the Diagnosis and Prognosis of Non-Ischemic Hypertensive Heart Disease
by Nikolaos P. E. Kadoglou, Angeliki Mouzarou, Nikoleta Hadjigeorgiou, Ioannis Korakianitis and Michael M. Myrianthefs
J. Clin. Med. 2024, 13(9), 2708; https://doi.org/10.3390/jcm13092708 (registering DOI) - 04 May 2024
Abstract
It has been well established that arterial hypertension is considered as a predominant risk factor for the development of cardiovascular diseases. Despite the link between arterial hypertension and cardiovascular diseases, arterial hypertension may directly affect cardiac function, leading to heart failure, mostly with [...] Read more.
It has been well established that arterial hypertension is considered as a predominant risk factor for the development of cardiovascular diseases. Despite the link between arterial hypertension and cardiovascular diseases, arterial hypertension may directly affect cardiac function, leading to heart failure, mostly with preserved ejection fraction (HFpEF). There are echocardiographic findings indicating hypertensive heart disease (HHD), defined as altered cardiac morphology (left ventricular concentric hypertrophy, left atrium dilatation) and function (systolic or diastolic dysfunction) in patients with persistent arterial hypertension irrespective of the cardiac pathologies to which it contributes, such as coronary artery disease and kidney function impairment. In addition to the classical echocardiographic parameters, novel indices, like speckle tracking of the left ventricle and left atrium, 3D volume evaluation, and myocardial work in echocardiography, may provide more accurate and reproducible diagnostic and prognostic data in patients with arterial hypertension. However, their use is still underappreciated. Early detection of and prompt therapy for HHD will greatly improve the prognosis. Hence, in the present review, we shed light on the role of echocardiography in the contemporary diagnostic and prognostic approaches to HHD. Full article
(This article belongs to the Section Cardiology)
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19 pages, 1528 KiB  
Article
Establishing Boundaries to Combat Tax Crimes in Indonesia
by Dwi Nurferyanto and Yoshi Takahashi
Laws 2024, 13(3), 29; https://doi.org/10.3390/laws13030029 (registering DOI) - 04 May 2024
Abstract
Enforcing criminal tax law in Indonesia presents a critical yet challenging task, because of the intricate interplay between tax and criminal law interests. The Indonesian Government has introduced leniency in tax criminal law enforcement, guided by the ultimum remedium principle, where criminal sanctions [...] Read more.
Enforcing criminal tax law in Indonesia presents a critical yet challenging task, because of the intricate interplay between tax and criminal law interests. The Indonesian Government has introduced leniency in tax criminal law enforcement, guided by the ultimum remedium principle, where criminal sanctions are considered as a last resort. Under this policy, tax offenders can absolve themselves from legal liability. However, such leniency throughout the enforcement process can lead to perceptions of injustice within society. This research uses descriptive, evaluative, and normative juridical methods to examine Indonesia’s approach to enforcing criminal tax laws within the framework of tax and legal interests. Our findings reveal that the current policies heavily favor taxpayer interests by providing numerous concessions to offenders. This trend is concerning, as it may result in a surge of tax crime cases. Conversely, adopting the primum remedium principle, where criminal sanctions are the initial response, poses the risk of harsh legal consequences. In light of these challenges, we propose a balanced approach incorporating elements of both ultimum and primum remedium principles to establish clear boundaries and provisions within criminal tax law enforcement policies. By doing so, we aim to accommodate tax interests while upholding legal interests. Full article
(This article belongs to the Section Criminal Justice Issues)
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21 pages, 508 KiB  
Review
Cosmetic Formulations from Natural Sources: Safety Considerations and Legislative Frameworks in the European Union
by Maame Ekua Manful, Lubna Ahmed and Catherine Barry-Ryan
Cosmetics 2024, 11(3), 72; https://doi.org/10.3390/cosmetics11030072 (registering DOI) - 04 May 2024
Abstract
Consumer preferences, safety, and sustainability aspects of conventional cosmetic ingredients have contributed to an increase in the demand for natural cosmetic ingredients and products. Naturally derived active cosmetic agents and excipients may come into contact with various naturally occurring and synthetic contaminants throughout [...] Read more.
Consumer preferences, safety, and sustainability aspects of conventional cosmetic ingredients have contributed to an increase in the demand for natural cosmetic ingredients and products. Naturally derived active cosmetic agents and excipients may come into contact with various naturally occurring and synthetic contaminants throughout the supply chain, and substantiating their safety is essential. This review examines the safety and legislative requirements applicable to natural cosmetic ingredients in the European Union (EU). Cosmetic safety requirements include technical data based on the ingredient profile, presence of hazards and the risks associated with the intended conditions of use. The hazard analysis includes screening for microbial contaminants such as aerobic mesophilic bacteria, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans; chemical contaminants such as lead, cadmium, arsenic, and mercury; and naturally occurring toxins, such as allergens. The toxicological assessment considers both local effects (such as skin sensitisation, eye/skin irritation, and photo-induced effects) and systemic effects (including acute dermal toxicity, sub-acute and sub-chronic toxicity, mutagenicity and carcinogenicity, reproductive toxicity, and toxicokinetics). The EU legislative requirements prohibit the use of animal-based tests for the toxicological evaluation of cosmetic ingredients, paving the way for alternatives termed as New Approach Methodologies (NAMs). The validation of NAMs is critical for their wider usage, and despite advancements, few have been validated, particularly for systemic toxicity testing. The use of NAMs in evaluating the safety of complex natural cosmetic ingredients is further examined. Full article
(This article belongs to the Special Issue Natural Sources for Cosmetic Ingredients: Challenges and Innovations)
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16 pages, 1330 KiB  
Article
Event-Triggered Synchronization of Coupled Neural Networks with Reaction–Diffusion Terms
by Abulajiang Aili, Shenglong Chen and Sibao Zhang
Mathematics 2024, 12(9), 1409; https://doi.org/10.3390/math12091409 (registering DOI) - 04 May 2024
Abstract
This paper focuses on the event-triggered synchronization of coupled neural networks with reaction–diffusion terms. At first, an effective event-triggered controller was designed based on time sampling. It is worth noting that the data of the controller for this type can be updated only [...] Read more.
This paper focuses on the event-triggered synchronization of coupled neural networks with reaction–diffusion terms. At first, an effective event-triggered controller was designed based on time sampling. It is worth noting that the data of the controller for this type can be updated only when corresponding triggering conditions are satisfied, which can significantly reduce the communication burden of the control systems compared to other control strategies. Furthermore, some sufficient criteria were obtained to ensure the event-triggered synchronization of the considered systems through the use of an inequality techniques as well as the designed controller. Finally, the validity of the theoretical results was confirmed using numerical examples. Full article
(This article belongs to the Special Issue Advances in Control Theory, Dynamic Systems, and Complex Networks)
20 pages, 476 KiB  
Article
Gender Asymmetries: An Exploratory Study of Women’s Experiences in Portuguese Football Organizations
by Maria Helena Santos, António Manuel Marques and Joana Salvador
Eur. J. Investig. Health Psychol. Educ. 2024, 14(5), 1228-1247; https://doi.org/10.3390/ejihpe14050081 (registering DOI) - 04 May 2024
Abstract
The main objective of this exploratory study is to analyze the negative effects associated with the phenomenon of tokenism for Portuguese women in non-playing football roles, as well as their strategies for integration into a context where they are a minority and the [...] Read more.
The main objective of this exploratory study is to analyze the negative effects associated with the phenomenon of tokenism for Portuguese women in non-playing football roles, as well as their strategies for integration into a context where they are a minority and the social functions are dominated by men. To this end, we carried out semi-structured individual interviews with eight men and eight women, undertaking functions in the fields of coaching, psychology, medical team, planning, refereeing, and management in football organizations, and with their contents then subject to thematic analysis. Our analysis confirms how the football context strongly demarcates gender differences with women being more visible and leads to the stereotypical roles being associated with traditional femininity. The findings also observe how, in order to integrate, women align with the norms imposed by the dominant group (men) and accept being circumscribed to the spaces that hegemonic gender ideologies and practices attribute to them. Full article
23 pages, 10457 KiB  
Article
Small-Scale Battery Energy Storage System for Testing Algorithms Aimed at Peak Power Reduction
by Krzysztof Sozański, Szymon Wermiński and Jacek Kaniewski
Energies 2024, 17(9), 2217; https://doi.org/10.3390/en17092217 (registering DOI) - 04 May 2024
Abstract
This study describes a laboratory model of a battery energy storage system (BESS) designed for testing algorithms aimed at reducing peak power consumption in railway traction substations. The system comprises a DC/DC converter and battery energy storage. This article details a laboratory model [...] Read more.
This study describes a laboratory model of a battery energy storage system (BESS) designed for testing algorithms aimed at reducing peak power consumption in railway traction substations. The system comprises a DC/DC converter and battery energy storage. This article details a laboratory model of a bidirectional buck-boost DC/DC converter, which is used to transfer energy between the battery energy storage and a DC line. It presents an analysis of DC/DC converter systems along with simulation studies. Furthermore, the results of laboratory tests on the DC/DC converter model are also provided. The control algorithm of the system in the traction substation is focused on reducing peak power, offering benefits such as lower charges for the railway operator due to the possibility of reducing contracted power requirements. From the perspective of the power grid, the reduction in power fluctuations and, consequently, voltage sags, is advantageous. This paper includes a description of a hardware simulator for verifying the system’s control algorithms. The verification of the control algorithms was performed through experimental tests conducted on a laboratory model (a hardware simulator) of the system for dynamic load reduction in traction substations, on a power scale of 1:1000 (5.5 kW). The experimental tests on the laboratory model (hardware simulator) demonstrated the effectiveness of the algorithm in reducing the peak power drawn from the power source. Full article
(This article belongs to the Collection Featured Papers in Electrical Power and Energy System)
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15 pages, 3035 KiB  
Article
Gut Mycobiome in Atopic Dermatitis and in Overweight Young Children: A Prospective Cohort Study in Finland
by Petri Vänni, Jenni Turunen, Ville K. Äijälä, Vilja V. Tapiainen, Marika Paalanne, Tytti Pokka, Niko Paalanne, Mysore V. Tejesvi and Terhi S. Ruuska
J. Fungi 2024, 10(5), 333; https://doi.org/10.3390/jof10050333 (registering DOI) - 04 May 2024
Abstract
Gut bacterial alterations have been previously linked to several non-communicable diseases in adults, while the association of mycobiome is not well understood in these diseases, especially in infants and children. Few studies have been conducted on the association between gut mycobiome and non-communicable [...] Read more.
Gut bacterial alterations have been previously linked to several non-communicable diseases in adults, while the association of mycobiome is not well understood in these diseases, especially in infants and children. Few studies have been conducted on the association between gut mycobiome and non-communicable diseases in children. We investigated gut mycobiome composition using 194 faecal samples collected at birth, 6 months after birth, and 18 months after birth in relation to atopic dermatitis (AD) and overweight diagnoses at the age of 18 or 36 months. The mycobiome exhibited distinct patterns, with Truncatella prevalent in the meconium samples of both overweight and non-overweight groups. Saccharomyces took precedence in overweight cases at 6 and 18 months, while Malassezia dominated non-overweight samples at 6 months. Saccharomyces emerged as a consistent high-abundance taxon across groups that had dermatitis and were overweight. We found a weak association between gut mycobiome and AD at birth and overweight at 18 months when using machine learning (ML) analyses. In ML, unidentified fungi, Alternaria, Rhodotorula, and Saccharomyces, were important for classifying AD, while Saccharomyces, Thelebolus, and Dothideomycetes were important for classifying overweight. Gut mycobiome might be associated with the development of AD and overweight in children. Full article
(This article belongs to the Special Issue Gut Mycobiome, 2nd Edition)
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12 pages, 1784 KiB  
Article
Investigation of Strategies to Block Downstream Effectors of AT1R-Mediated Signalling to Prevent Aneurysm Formation in Marfan Syndrome
by Irene Valdivia Callejon, Lucia Buccioli, Jarl Bastianen, Jolien Schippers, Aline Verstraeten, Ilse Luyckx, Silke Peeters, Jan A. H. Danser, Roland R. J. Van Kimmenade, Josephina Meester and Bart Loeys
Int. J. Mol. Sci. 2024, 25(9), 5025; https://doi.org/10.3390/ijms25095025 (registering DOI) - 04 May 2024
Abstract
Cardiovascular outcome in Marfan syndrome (MFS) patients most prominently depends on aortic aneurysm progression with subsequent aortic dissection. Angiotensin II receptor blockers (ARBs) prevent aneurysm formation in MFS mouse models. In patients, ARBs only slow down aortic dilation. Downstream signalling from the angiotensin [...] Read more.
Cardiovascular outcome in Marfan syndrome (MFS) patients most prominently depends on aortic aneurysm progression with subsequent aortic dissection. Angiotensin II receptor blockers (ARBs) prevent aneurysm formation in MFS mouse models. In patients, ARBs only slow down aortic dilation. Downstream signalling from the angiotensin II type 1 receptor (AT1R) is mediated by G proteins and β-arrestin recruitment. AT1R also interacts with the monocyte chemoattractant protein-1 (MCP-1) receptor, resulting in inflammation. In this study, we explore the targeting of β-arrestin signalling in MFS mice by administering TRV027. Furthermore, because high doses of the ARB losartan, which has been proven beneficial in MFS, cannot be achieved in humans, we investigate a potential additive effect by combining lower concentrations of losartan (25 mg/kg/day and 5 mg/kg/day) with barbadin, a β-arrestin blocker, and DMX20, a C-C chemokine receptor type 2 (CCR2) blocker. A high dose of losartan (50 mg/kg/day) slowed down aneurysm progression compared to untreated MFS mice (1.73 ± 0.12 vs. 1.96 ± 0.08 mm, p = 0.0033). TRV027, the combination of barbadin with losartan (25 mg/kg/day), and DMX-200 (90 mg/kg/day) with a low dose of losartan (5 mg/kg/day) did not show a significant beneficial effect. Our results confirm that while losartan effectively halts aneurysm formation in Fbn1C1041G/+ MFS mice, neither TRV027 alone nor any of the other compounds combined with lower doses of losartan demonstrate a notable impact on aneurysm advancement. It appears that complete blockade of AT1R function, achieved by administrating a high dosage of losartan, may be necessary for inhibiting aneurysm progression in MFS. Full article
(This article belongs to the Special Issue Molecular Pharmacology and Interventions in Cardiovascular Disease)
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11 pages, 250 KiB  
Article
Generalizability Theory in the Evaluation of Psychological Profile in Track and Field
by Cristina Sanz-Fernández, Verónica Morales-Sánchez, Julen Castellano and Antonio Hernández Mendo
Sports 2024, 12(5), 127; https://doi.org/10.3390/sports12050127 (registering DOI) - 04 May 2024
Abstract
Generalizability theory (GT) has been used throughout the scientific literature to ensure validity, reliability, and generalizability in different sport contexts. However, there is a small number of studies examining the measurement of psychological profiles in sport from this perspective. Therefore, this study’s main [...] Read more.
Generalizability theory (GT) has been used throughout the scientific literature to ensure validity, reliability, and generalizability in different sport contexts. However, there is a small number of studies examining the measurement of psychological profiles in sport from this perspective. Therefore, this study’s main goal is the sources of variability and the optimal measurement design estimation for a good assessment of the psychological profile in track and field. The sample consisted of 470 participants (age: Average= 32.1; Standar Desviation = 13.5). The analysis of variance and generalizability component analysis has been performed in order to test the reliability and generalizability of the sample. The profile included the following variables: flow, motivation (from Self-Determination Theory and Achievement Goals), self-confidence, and psychological skills. Results confirm that the sample has a high degree of reliability and generalizability in all the tested models. So, a detailed study on the validity, reliability, and generalizability of samples and measures should be an inherent element in the practice of psychological counseling in sports. Full article
29 pages, 878 KiB  
Review
Application of Developmental Regulators for Enhancing Plant Regeneration and Genetic Transformation
by Pingjun Xu, Yinxiao Zhong, Ang Xu, Bingshuang Liu, Yue Zhang, Anqi Zhao, Xiaoming Yang, Meiling Ming, Fuliang Cao and Fangfang Fu
Plants 2024, 13(9), 1272; https://doi.org/10.3390/plants13091272 (registering DOI) - 04 May 2024
Abstract
Establishing plant regeneration systems and efficient genetic transformation techniques plays a crucial role in plant functional genomics research and the development of new crop varieties. The inefficient methods of transformation and regeneration of recalcitrant species and the genetic dependence of the transformation process [...] Read more.
Establishing plant regeneration systems and efficient genetic transformation techniques plays a crucial role in plant functional genomics research and the development of new crop varieties. The inefficient methods of transformation and regeneration of recalcitrant species and the genetic dependence of the transformation process remain major obstacles. With the advancement of plant meristematic tissues and somatic embryogenesis research, several key regulatory genes, collectively known as developmental regulators, have been identified. In the field of plant genetic transformation, the application of developmental regulators has recently garnered significant interest. These regulators play important roles in plant growth and development, and when applied in plant genetic transformation, they can effectively enhance the induction and regeneration capabilities of plant meristematic tissues, thus providing important opportunities for improving genetic transformation efficiency. This review focuses on the introduction of several commonly used developmental regulators. By gaining an in-depth understanding of and applying these developmental regulators, it is possible to further enhance the efficiency and success rate of plant genetic transformation, providing strong support for plant breeding and genetic engineering research. Full article
16 pages, 3003 KiB  
Article
Safety and Immunogenicity of an Accelerated Ebola Vaccination Schedule in People with and without Human Immunodeficiency Virus: A Randomized Clinical Trial
by Julie A. Ake, Kristopher Paolino, Jack N. Hutter, Susan Biggs Cicatelli, Leigh Anne Eller, Michael A. Eller, Margaret C. Costanzo, Dominic Paquin-Proulx, Merlin L. Robb, Chi L. Tran, Lalaine Anova, Linda L. Jagodzinski, Lucy A. Ward, Nicole Kilgore, Janice Rusnak, Callie Bounds, Christopher S. Badorrek, Jay W. Hooper, Steven A. Kwilas, Ine Ilsbroux, Dickson Nkafu Anumendem, Auguste Gaddah, Georgi Shukarev, Viki Bockstal, Kerstin Luhn, Macaya Douoguih and Cynthia Robinsonadd Show full author list remove Hide full author list
Vaccines 2024, 12(5), 497; https://doi.org/10.3390/vaccines12050497 (registering DOI) - 04 May 2024
Abstract
The safety and immunogenicity of the two-dose Ebola vaccine regimen MVA-BN-Filo, Ad26.ZEBOV, 14 days apart, was evaluated in people without HIV (PWOH) and living with HIV (PLWH). In this observer-blind, placebo-controlled, phase 2 trial, healthy adults were randomized (4:1) to receive MVA-BN-Filo (dose [...] Read more.
The safety and immunogenicity of the two-dose Ebola vaccine regimen MVA-BN-Filo, Ad26.ZEBOV, 14 days apart, was evaluated in people without HIV (PWOH) and living with HIV (PLWH). In this observer-blind, placebo-controlled, phase 2 trial, healthy adults were randomized (4:1) to receive MVA-BN-Filo (dose 1) and Ad26.ZEBOV (dose 2), or two doses of saline/placebo, administered intramuscularly 14 days apart. The primary endpoints were safety (adverse events (AEs)) and immunogenicity (Ebola virus (EBOV) glycoprotein-specific binding antibody responses). Among 75 participants (n = 50 PWOH; n = 25 PLWH), 37% were female, the mean age was 44 years, and 56% were Black/African American. AEs were generally mild/moderate, with no vaccine-related serious AEs. At 21 days post-dose 2, EBOV glycoprotein-specific binding antibody responder rates were 100% among PWOH and 95% among PLWH; geometric mean antibody concentrations were 6286 EU/mL (n = 36) and 2005 EU/mL (n = 19), respectively. A total of 45 neutralizing and other functional antibody responses were frequently observed. Ebola-specific CD4+ and CD8+ T-cell responses were polyfunctional and durable to at least 12 months post-dose 2. The regimen was well tolerated and generated robust, durable immune responses in PWOH and PLWH. Findings support continued evaluation of accelerated vaccine schedules for rapid deployment in populations at immediate risk. Trial registration: NCT02598388 (submitted 14 November 2015). Full article
20 pages, 3470 KiB  
Article
Overt Word Reading and Visual Object Naming in Adults with Dyslexia: Electroencephalography Study in Transparent Orthography
by Maja Perkušić Čović, Igor Vujović, Joško Šoda, Marijan Palmović and Maja Rogić Vidaković
Bioengineering 2024, 11(5), 459; https://doi.org/10.3390/bioengineering11050459 (registering DOI) - 04 May 2024
Abstract
The study aimed to investigate overt reading and naming processes in adult people with dyslexia (PDs) in shallow (transparent) language orthography. The results of adult PDs are compared with adult healthy controls HCs. Comparisons are made in three phases: pre-lexical (150–260 ms), lexical [...] Read more.
The study aimed to investigate overt reading and naming processes in adult people with dyslexia (PDs) in shallow (transparent) language orthography. The results of adult PDs are compared with adult healthy controls HCs. Comparisons are made in three phases: pre-lexical (150–260 ms), lexical (280–700 ms), and post-lexical stage of processing (750–1000 ms) time window. Twelve PDs and HCs performed overt reading and naming tasks under EEG recording. The word reading and naming task consisted of sparse neighborhoods with closed phonemic onset (words/objects sharing the same onset). For the analysis of the mean ERP amplitude for pre-lexical, lexical, and post-lexical time window, a mixed design ANOVA was performed with the right (F4, FC2, FC6, C4, T8, CP2, CP6, P4) and left (F3, FC5, FC1, T7, C3, CP5, CP1, P7, P3) electrode sites, within-subject factors and group (PD vs. HC) as between-subject factor. Behavioral response latency results revealed significantly prolonged reading latency between HCs and PDs, while no difference was detected in naming response latency. ERP differences were found between PDs and HCs in the right hemisphere’s pre-lexical time window (160–200 ms) for word reading aloud. For visual object naming aloud, ERP differences were found between PDs and HCs in the right hemisphere’s post-lexical time window (900–1000 ms). The present study demonstrated different distributions of the electric field at the scalp in specific time windows between two groups in the right hemisphere in both word reading and visual object naming aloud, suggesting alternative processing strategies in adult PDs. These results indirectly support the view that adult PDs in shallow language orthography probably rely on the grapho-phonological route during overt word reading and have difficulties with phoneme and word retrieval during overt visual object naming in adulthood. Full article
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20 pages, 5994 KiB  
Article
Numerical Analysis of the Stress Shadow Effects in Multistage Hydrofracturing Considering Natural Fracture and Leak-Off Effect
by Jinxin Song, Qing Qiao, Chao Chen, Jiangtao Zheng and Yongliang Wang
Water 2024, 16(9), 1308; https://doi.org/10.3390/w16091308 (registering DOI) - 04 May 2024
Abstract
As a critical technological approach, multistage fracturing is frequently used to boost gas recovery in compact hydrocarbon reservoirs. Determining an ideal cluster distance that effectively integrates pre-existing natural fractures in the deposit creates a fracture network conducive to gas movement. Fracturing fluid leak-off [...] Read more.
As a critical technological approach, multistage fracturing is frequently used to boost gas recovery in compact hydrocarbon reservoirs. Determining an ideal cluster distance that effectively integrates pre-existing natural fractures in the deposit creates a fracture network conducive to gas movement. Fracturing fluid leak-off also impacts water resources. In our study, we use a versatile finite element–discrete element method that improves the auto-refinement of the grid and the detection of multiple fracture movements to model staged fracturing in naturally fractured reservoirs. This computational model illustrates the interaction between hydraulic fractures and pre-existing fractures and employs the nonlinear Carter leak-off criterion to portray fluid leakage and the impacts of hydromechanical coupling during multistage fracturing. Numerical results show that sequential fracturing exhibits the maximum length in unfractured and naturally fractured models, and the leak-off volume of parallel fracturing is the smallest. Our study proposes an innovative technique for identifying and optimizing the spacing of fracturing clusters in unconventional reservoirs. Full article
(This article belongs to the Special Issue Thermo-Hydro-Mechanical Coupling in Fractured Porous Media)
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18 pages, 5894 KiB  
Article
Molecular Simulations of Unexplored Philippine Plant Constituents on the Inhibition of the Proinflammatory Marker NF-κB p50 Subunit
by Jasmine U. Ting, Maria Carmen S. Tan, Vincent Antonio S. Ng, Stephani Joy Y. Macalino, Virgilio C. Linis and Glenn G. Oyong
Crystals 2024, 14(5), 438; https://doi.org/10.3390/cryst14050438 (registering DOI) - 04 May 2024
Abstract
Inflammation serves as a pivotal defense mechanism orchestrated by the innate immune system to safeguard cellular health against adversities. Nonetheless, dysregulated inflammatory responses can precipitate chronic inflammatory ailments, notably autoimmune disorders. Central to this process are various pathways, with studies highlighting the pivotal [...] Read more.
Inflammation serves as a pivotal defense mechanism orchestrated by the innate immune system to safeguard cellular health against adversities. Nonetheless, dysregulated inflammatory responses can precipitate chronic inflammatory ailments, notably autoimmune disorders. Central to this process are various pathways, with studies highlighting the pivotal role of transcription factors within the nuclear factor-kappa B (NF-κB) signaling pathway in disease onset and progression. This study concentrates on the p50 homodimer protein, a key transcription factor pivotal for the expression of proinflammatory cytokine genes. To explore potential inhibitors of p50, we conducted in silico procedures to investigate fifty-eight unexplored compounds, derived from plants indigenous to the Philippines. Initial screenings for compound feasibility, through drug-likeness analyses, yielded positive outcomes for 34 compounds. Subsequent docking analyses revealed six compounds exhibiting binding energies (ranging from −3.7 to −4.2 kcal/mol) akin to or lower than the positive control, dexamethasone (−3.7 kcal/mol). These compounds include eudesm-11-en-4α-O-β-D-3-tigoyloxy-6-deoxy-glucopyranoside, wadeiol, grandiflorolide, eudesm-11-en-4α-O-β-D-3-senecioyloxy-6-deoxyglucopyranoside, α-pinene-7β-O-β-D-2- acetylglucopyranoside, and (2aβ,3α,5aβ,6β,7α,8aα)-6-[2-(3-furanyl)ethyl]-2a,3,4,5,5a,6,7,8,8a,8b- decahydro-2a,3-dihydroxy-6,7,8b-trimethyl-2H-naphtho[1-8-bc]furan-2-one. Interaction analyses revealed a common engagement of amino acid residues within the p50 DNA binding pocket, notably Arg57, Tyr60, Glu63, Lys244, Ala245, Pro246, Lys275, Arg308, Gln309, and Phe310, through hydrogen bonding, van der Waals forces, alkyl, and pi–alkyl interactions. Pharmacophore analysis underscored aromatic rings, hydroxyl, methyl, and methylene groups as pivotal for non-covalent interactions with p50. Additionally, root mean square fluctuation (RMSF) analysis demonstrated minimal residue fluctuations in p50 upon ligand binding compared to the ligand-free protein structure. In conclusion, the six shortlisted compounds exhibiting comparable binding affinities with dexamethasone hold promise as potential anti-inflammatory agents targeting the NF-κB p50 homodimer. Full article
(This article belongs to the Section Biomolecular Crystals)
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21 pages, 2937 KiB  
Article
Circularity Assessment in Aquaculture: The Case of Integrated Multi-Trophic Aquaculture (IMTA) Systems
by Daniel Checa, Brett M. Macey, John J. Bolton, Marissa Brink-Hull, Pauline O’Donohoe, Alessandro Cardozo, Luis Henrique Poersch and Inmaculada Sánchez
Fishes 2024, 9(5), 165; https://doi.org/10.3390/fishes9050165 (registering DOI) - 04 May 2024
Abstract
Aquaculture is a strategic sector that aims to meet the increased demands for healthy food for current and future populations. However, this progression needs to be sustainable, which can potentially be achieved by the implementation of circular practices. Integrated multi-trophic aquaculture (IMTA) systems [...] Read more.
Aquaculture is a strategic sector that aims to meet the increased demands for healthy food for current and future populations. However, this progression needs to be sustainable, which can potentially be achieved by the implementation of circular practices. Integrated multi-trophic aquaculture (IMTA) systems promote the incorporation of circular principles. Nevertheless, the lack of harmonized definitions and standards impedes the quantification of these circular attributes. This study aims to explore the potential principles embedded in IMTA and the existing alternatives to quantify circularity. Two basic pillars (nutrient management and resource use efficiency) were identified as the most relevant circularity attributes for IMTA systems and were quantified through aquaculture-specific indicators. Bioremediation indicators, together with the efficiency indicators in terms of feed, water, energy, and infrastructure materials used, were selected to evaluate the circularity performance of four IMTA trials in three aquaculture facilities in Ireland, Brazil, and South Africa. Salmon, white shrimp, tilapia, abalone, and sea urchins were studied and cultivated together in various combinations with several low-trophic species in these IMTA trials to evaluate the improvement in circularity compared with corresponding monoculture conditions. The results showed an increase in circularity of up to 90% in terms of water recirculation, as well as bioremediation, which was improved by 80%–90%, providing evidence for the potential role of IMTA in the circularity transition. Full article
(This article belongs to the Special Issue Integrated Aquaculture and Monoculture of Low-Trophic Species)
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