A 3D-finite element analysis within the numerical program ABAQUS is adopted in order to simulate the seismic behavior of reinforced concrete beam-column joints and beam-column joints strengthened with CFRP ropes. The suitability of the adopted approach is investigated herein. For this purpose, experimental and numerical cyclic tests were performed. The experiments include four reinforced concrete (RC) joints with the same ratio of shear closed-stirrup reinforcement and two different volumetric ratios of longitudinal steel reinforcing bars. Two joints were tested as-built, and the other two were strengthened with CFRP ropes. The ropes were applied as Near Surface Mounted (NSM) reinforcement, forming an X-shape around the joint body and further as flexural reinforcement at the top and bottom of the beam. The purpose of the externally mounted CFRP ropes is to allow the development of higher values of concrete principal stresses inside the joint core, compared with the specimens without ropes, and also to reduce the developing shear deformation in the joint. From the results, it is concluded that X-shaped ropes reduced the shear deformation in the joint body remarkably, especially in high drifts. Further, as a result of the comparisons between the yielded outcome from the attempted nonlinear analysis and the observed response from the tests, it is deduced that the adopted method sufficiently describes the whole behavior of the RC beam-column connections. In particular, comparisons between experimental and numerical results of principal stresses developing in the joint body of all examined specimens, along with similar comparisons of force displacement envelopes and shear deformations of the joint body, confirmed the adequacy of the applied finite element approach for the investigation of the use of CFRP-ropes as an efficient and easy-to-apply strengthening technique. The findings also reveal that the connections that have been strengthened with the FRP ropes demonstrated improved performance, and the crack system preserved its load capacity during the reversal loading tests. Full article

Scientific experiments cover a wide range of fields—from basic to applied scientific research. Chemical experiments are the basis for cultivating chemical knowledge in scientific experiments and are an important way to cultivate scientific thinking and methods. However, due to the toxicity or flammability of the chemical substances in the experiments, hazardous events often lead to personal injuries and environmental damage. Exactly assessing risk factors and reducing the risk of hazards to protect the experimenters and ensure environmental safety are crucial in chemical experiments. However, while the traditional risk evaluation method cannot consider the weight of risk evaluation criteria, it also cannot effectively address problems through hierarchical analysis, as well as imprecise and ambiguous information inherent in human cognition. Therefore, this paper proposed an approach based on failure mode and effects analysis (FMEA) to assess the risk of chemical experiments in a fuzzy information environment. The approach combines the typical analytic hierarchy process (AHP), the risk priority number (RPN) of FMEA, and the intuitionistic fuzzy set (IFS) methods to evaluate risks associated with chemical experiments and consider the damage recovery in chemical experiments. This study applied the case of a university chemistry experiment, “preparation of hydrogels”, to validate the reasonableness and correctness of the proposed approach and compare its numerical verification results with those from the typical RPN, the AHP-RPN, and the AHP-fuzzy risk priority number (AHP-FRPN) methods. The finding demonstrates that the proposed method can more effectively address risk evaluation problems in chemical experiments than the other methods. This result serves as an important reference for reducing chemical experiment risk occurrences. Full article

As environmental plastic waste degrades, it creates an abundance of diverse microplastic particles. Consequently, microplastics contaminate drinking water and many staple food products, meaning the oral ingestion of microplastics is an important exposure route for the human population. Microplastics have long been considered inert, however their ability to promote microbial dysbiosis as well as gut inflammation and dysfunction suggests they are more noxious than first thought. More alarmingly, there is evidence for microplastics permeating from the gut throughout the body, with adverse effects on the immune and nervous systems. Coupled with the now-accepted role of the gut-brain axis in neurodegeneration, these findings support the hypothesis that this ubiquitous environmental pollutant is contributing to the rising incidence of neurodegenerative diseases, like Alzheimer’s disease and Parkinson’s disease. This comprehensive narrative review explores the consequences of oral microplastic exposure on the gut-brain-axis by considering current evidence for gastrointestinal uptake and disruption, immune activation, translocation throughout the body, and neurological effects. As microplastics are now a permanent feature of the global environment, understanding their effects on the gut, brain, and whole body will facilitate critical further research and inform policy changes aimed at reducing any adverse consequences. Full article

The pine wood nematode (PWN) uses several Monochamus species as vehicles, through a temporary hitchhiking process known as phoresy, enabling it to access new host plant resources. Monochamus saltuarius acts as a new and major vector of the PWN in Northeastern China, showing lower PWN carrying capacity and a shorter transmission cycle compared to established vectors. The apparently altered symbiotic relationship offers an interesting area for researching the costs and adaptions involved in nematode–beetle, a specialized phoresy. We analyzed the response and fitness costs of M. saltuarius through physiological measurements and transcriptomics. The PWN exerted adverse repercussions on the growth and development of M. saltuarius. The PWN accelerated larval development into pupae, while beetle adults carrying the PWN exhibited an elevated abnormality rate and mortality, and reduced starvation resistance. During the pupal stage, the expression of growth-related genes, including ecdysone-inducible genes (E74EA), cuticle proteins, and chitin genes (CHTs), markedly increased. Meanwhile, the induced immune response, mainly by the IMD and Toll signaling pathways, could be a contributing factor to adult abnormality and mortality. Adult gonads and trachea exhibited enrichment in pathways related to fatty acid elongation, biosynthesis, and metabolism. FASN, ELOVL, and SCD possibly contributed to resistance against PWN. Our research indicated that phoretic interactions between vector beetles and PWN vary throughout the vector’s lifespan, particularly before and after entry into the trachea. This study highlighted the fitness costs of immunity and metabolism on the vector beetle, indicating the adaptation mechanisms and evolutionary trade-offs to PWN. Full article

This study provides pioneering research on the vegetation of archaeological areas in Iran to enhance its naturalistic and bioindication values by selecting the Pasargadae World Heritage Site (WHS). Vegetation surveys were carried out in different homogeneous habitats, analyzing the plant communities through statistical elaboration, syntaxonomic role, mapping, and enhancement of plants with conservation interest. In an ecological approach, the study included an analysis of the recent climate changes and human interventions influencing the water resources. Results revealed seven main vegetation types reflecting ecological gradients shaped by environmental, edaphic, and anthropogenic factors. The syntaxonomic analysis showed a primary subdivision in semi-natural grasslands and synanthropic vegetation. Several key species were identified as bioindicators of multiple factors, such as: Launaea acanthodes, Stipa barbata, Alhagi maurorum, Bellevalia saviczii, Glycyrrhiza glabra, Convolvulus arvensis, and Hordeum murinum. The vegetation map showed how the hilly grassland communities hosted the highest number of species with conservation interest and their need to be better protected. Bio-climatic data, such as the construction of dams and the exploitation for irrigation purposes, pointed to the increasing xeric conditions, which make urging conservation efforts for the site’s historical and naturalistic values. The study underscores the importance of preserving places with high plant diversity for effective site management, and enhances the intricate relationship between vegetation and natural features in the occurring environmental changes. Full article

Exosome-based therapy has emerged as a promising strategy for addressing diverse disorders, indicating the need for further exploration of the potential therapeutic effects of the exosome cargos. This study introduces “enhanced exosomes”, a novel type of exosomes developed through a novel cell culture system. These specific exosomes may become potent therapeutic agents for treating ovarian disorders. In this study, we conducted a comparative analysis of the protein and miRNA cargo compositions of enhanced exosomes and naïve exosomes. Our findings revealed distinct cargo compositions in enhanced exosomes, featuring upregulated proteins such as EFEMP1, HtrA1, PAM, and SDF4, suggesting their potential for treating ovarian disorders. MicroRNA profiling revealed that miR-1-3p, miR-103a-3p, miR-122-5p, miR-1271-5p, miR-133a-3p, miR-184, miR-203a-3p, and miR-206 are key players in regulating ovarian cancer and chemosensitivity by affecting cell cycle progression, cell proliferation, and cell development. We examined polycystic ovary syndrome and premature ovarian insufficiency and identified the altered expression of various miRNAs, such as miR-125b-5p and miR-130b-3p, for diagnostic insights. This study highlights the potential of enhanced exosomes as new therapeutic agents for women’s reproductive health, offering a detailed understanding of the impact of their cargo on ovarian disorders. Full article

A series of experiments has been conducted to investigate the tribological properties of a TiN film sliding against GCr15 steel balls in ambient air, low vacuum and high vacuum environments. Various friction loads and sliding velocities were also applied. The TiN film displays a steady-state friction stage after the running-in stage in all the above environments, while the durations of running-in stages are different. The steady-state friction coefficients of the TiN film were around 0.56 in ambient air and 0.3 in the high vacuum environment (1 × 10⁻⁵ mbar). In the low vacuum (1 × 10⁻² mbar) environment, a low friction coefficient (around 0.19) was attained for all the friction tests on TiN film, irrespective of the applied load and sliding velocity. In the meantime, it was noticed that the applied loads and the sliding velocities would change the duration of the running-in stage before reaching the low friction coefficient. It is revealed by the analysis of wear tracks that the metal oxides induced by the tribo-chemical effect at the friction interface play an important role in affecting the tribological behaviors of the TiN films in different environments. The Raman results show that the main component of the metal oxides is hematite (α-Fe₂O₃), and the amount of iron oxide is related to the friction environment. The composition and quantity of iron oxides produced by the interfacial tribo-chemical effect affect the tribological behavior. The results also show that the mechanical wear process at the friction interface displays a polishing effect, which would reduce the surface roughness. The mechanical wear performance varies under different loads and velocities. The tribological tests results indicate that the interfacial tribo-chemical effect and mechanical wear process should be considered together rather than individually to interpret the tribological behaviors of TiN films in different environments. Full article

Carrageenan is a widely used food additive and is seen as a potential candidate in the pharmaceutical industry. However, there are two faces to carrageenan that allows it to be used positively for therapeutic purposes. Carrageenan can be used to create edible films and for encapsulating drugs, and there is also interest in the use of carrageenan for food printing. Carrageenan is a naturally occurring polysaccharide gum. Depending on the type of carrageenan, it is used in regulating the composition of intestinal microflora, including the increase in the population of Bifidobacterium bacteria. On the other hand, the studies have demonstrated the harmfulness of carrageenan in animal and human models, indicating a direct link between diet and intestinal inflammatory states. Carrageenan changes the intestinal microflora, especially Akkermansia muciniphilia, degrades the mucous barrier and breaks down the mucous barrier, causing an inflammatory reaction. It directly affects epithelial cells by activating the pro-inflammatory nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) pathway. The mechanism is based on activation of the TLR4 receptor, alterations in macrophage activity, production of proinflammatory cytokines and activation of innate immune pathways. Carrageenan increases the content of Bacteroidetes bacteria, also causing a reduction in the number of short chain fatty acid (SCFA)-producing bacteria. The result is damage to the integrity of the intestinal membrane and reduction of the mucin layer. The group most exposed to the harmful effects of carrageenan are people suffering from intestinal inflammation, including Crohn disease (CD) and ulcerative colitis (UC). Full article

Aim: This study aimed to compare the torque loss, fracture load, compressive strength, and failure types of selective-laser-sintered cobalt chromium (SLM-Co-Cr), computer-aided design and computer-aided manufacturing zirconium oxide (CAD-CAM-ZrO), and machined titanium (Ti) implant abutments. Methods: Thirty endosseous dental implants were vertically embedded with machined Ti (control group), CAD-CAM-ZrO, and SLM-Co-Cr abutments. Abutment fabrication involved CAD-CAM milling and SLM technology. The de-torque assessment included preload reverse torque values (RTVs), cyclic loading, and post-RTVs using a customized protocol. Fracture load assessment employed ISO-14801 standards, and statistical analysis was conducted using ANOVA and Tukey Post hoc tests (p < 0.05). Results: In pre-load RTVs, SLM-Co-Cr showed the lowest mean torque loss (24.30 ± 2.13), followed by machined Ti (27.33 ± 2.74) and CAD-CAM-ZrO (22.07 ± 2.20). Post-load RTVs decreased for all groups. Fracture load and compressive strength were highest for SLM-Co-Cr, with significant differences among groups (p < 0.001). Fracture types included abutment failures in SLM-Co-Cr and machined Ti, while CAD-CAM-ZrO exhibited crown separation with deformation. Conclusion: SLM-Co-Cr-fabricated implant abutments exhibited superior stability and resistance to rotational forces, higher fracture loads, and greater compressive strength compared to CAD-CAM-ZrO and machined Ti. Full article

Recent studies have found increased cardiovascular mortality risk in patients with type 1 diabetes when compared to normoglycemic people, even when they were kept under good glycemic control. However, the mechanisms underlying this condition have yet to be fully understood. Using streptozotocin (STZ)-induced diabetic rats, we evaluated the effects of insulin replacement therapy on cardiac, autonomic, inflammatory, and oxidative stress parameters. Daily treatment with insulin administrated subcutaneously in the STZ-diabetic rats showed a reduction in hyperglycemia (>250 mg/dL) to normalized values. The insulin treatment was effective in preventing alterations in cardiac morphometry and systolic function but had no impact on diastolic function. Also, the treatment was not able to prevent the impairment of baroreflex-tachycardic response and systolic arterial pressure variability (SAP-V). A correlation was found between improvement of these autonomic parameters and higher levels of IL-10 and lower levels of oxidized glutathione. Our findings show that insulin treatment was not able to prevent diastolic, baroreflex, and SAP-V dysfunction, suggesting an outstanding cardiovascular risk, even after obtaining a good glycemic control in STZ-induced diabetic rats. This study shed light on a relatively large population of diabetic patients in need of other therapies to be used in combination with insulin treatment and thus more effectively manage cardiovascular risk. Full article

Background: tinnitus is a common and often debilitating condition with limited evidence-based treatment options. Deep brain stimulation (DBS) is an approved treatment modality for certain neurological conditions; its experimental use as a treatment modality for severe tinnitus is novel and beginning to show promise. This systematic review focuses on the current evidence for the safety and efficacy of DBS for treatment of refractory tinnitus. Methods: a systematic search in PubMed and EMBASE was performed to identify peer-reviewed studies on DBS of non-cortical structures for the primary indication of tinnitus treatment. Three studies were identified as meeting these criteria, one of which had two related sub-studies. Results: seven patients with available data who underwent DBS for tinnitus were identified. DBS targets included nucleus accumbens (NAc), ventral anterior limb of the internal capsule (vALIC), caudate nucleus, and the medial geniculate body (MGB) of the thalamus. All studies used the Tinnitus Functional Index (TFI) as a primary outcome measure. DBS of the caudate was most commonly reported (n = 5), with a mean TFI improvement of 23.3 points. Only one subject underwent DBS targeting the NAc/vALIC (extrapolated TFI improvement 46.8) and one subject underwent DBS targeting the MGB (TFI improvement 59 points). Conclusions: DBS is a promising treatment option for refractory subjective tinnitus, with early data, from small patient cohorts in multiple studies, suggesting its safety and efficacy. Further studies with a larger patient population are needed to support this safety and efficacy before implementing this treatment to daily practice. Full article

This comprehensive literature review investigates the impact of stabilization and reinforcement techniques on the mechanical, hygrothermal properties, and durability of adobe and compressed earth blocks (CEBs). Recent advancements in understanding these properties have spurred a burgeoning body of research, prompting a meticulous analysis of 70 journal articles and conference proceedings. The selection criteria focused on key parameters including construction method (block type), incorporation of natural fibers or powders, partial or complete cement replacement, pressing techniques, and block preparation methods (adobe or CEB). The findings unearth several significant trends. Foremost, there is a prevailing interest in utilizing waste materials, such as plant matter, construction and demolition waste, and mining by-products, to fortify or stabilize earth blocks. Additionally, the incorporation of natural fibers manifests in a discernible reduction in crack size attributable to shrinkage, accompanied by enhancements in durability, mechanical strength, and thermal resistance. Moreover, this review underscores the imperative of methodological coherence among researchers to facilitate scalable and transposable results. Challenges emerge from the variability in base soil granulometry and disparate research standards, necessitating concerted efforts to harness findings effectively. Furthermore, this review illuminates a gap in complete lifecycle analyses of earthen structures, underscoring the critical necessity for further research to address this shortfall. It emphasizes the urgent need for deeper exploration of properties and sustainability indicators, recognizing the inherent potential and enduring relevance of earthen materials in fostering sustainable development. This synthesis significantly contributes to the advancement of knowledge in the field and underscores the continued importance of earth-based construction methodologies in contemporary sustainable practices. Full article

Background: SARS-Co-V2 infection can induce ER stress-associated activation of unfolded protein response (UPR) in host cells, which may contribute to the pathogenesis of COVID-19. To understand the complex interplay between SARS-Co-V2 infection and UPR signaling, we examined the effects of acute pre-existing ER stress on SARS-Co-V2 infectivity. Methods: Huh-7 cells were treated with Tunicamycin (TUN) and Thapsigargin (THA) prior to SARS-CoV-2pp transduction (48h p.i.) to induce ER stress. Pseudo-typed particles (SARS-CoV-2pp) entry into host cells was measured by Bright Glo^TM luciferase assay. Cell viability was assessed by cell titer Glo^® luminescent assay. The mRNA and protein expression was evaluated by RT-qPCR and Western Blot. Results: TUN (5 µg/ml) and THA (1 µM) efficiently inhibited the entry of SARS-CoV-2pp into host cells without any cytotoxic effect. TUN and THA's attenuation of virus entry was associated with differential modulation of ACE2 expression. Both TUN and THA significantly reduced the expression of stress-inducible ER chaperone GRP78/BiP in transduced cells. In contrast, the IRE1-XBP1s and PERK-eIF2α-ATF4-CHOP signaling pathways were downregulated with THA treatment, but not TUN in transduced cells. Insulin-mediated glucose uptake and phosphorylation of Ser³⁰⁷ IRS-1 and downstream p-AKT were enhanced with THA in transduced cells. Furthermore, TUN and THA differentially affected lipid metabolism and apoptotic signaling pathways. Conclusions: These findings suggest that short-term pre-existing ER stress prior to virus infection induces a specific UPR response in host cells capable of counteracting stress-inducible elements signaling, thereby depriving SARS-Co-V2 of essential components for entry and replication. Pharmacological manipulation of ER stress in host cells might provide new therapeutic strategies to alleviate SARS-CoV-2 infection. Full article

Common bean (Phaseolus vulgaris L.), one of the most important cultivated legumes, requires a high level of water. It is included among the most sensitive species to climate change; drought and salinity cause a reduction in photosynthesis, metabolic and enzymatic alterations, and oxidative stress. To improve crop tolerance to salt, seed priming and acclimation can be useful tools. To test the salt tolerance of beans, a preliminary screening was undertaken on four cultivars of P. vulgaris (Black Turtle, Cargamanto, Bola Roja, Borlotto) by exposing the seeds to different levels of salinity. The salt-sensitive cultivar Borlotto was chosen for experimental greenhouse trials to study the effects of halopriming and acclimation. Primed and non-primed seeds were sown in non-saline soil and acclimated for 2 weeks; then, the plants were watered with non-saline and saline solutions for 4 weeks. At the end of this growth period, the primed plants showed a marked increase in salt stress tolerance, improving the chlorophyll content, phenolic compounds, and many enzymes’ activities, in turn reducing the effect of salt on growth and fruit production compared to the non-primed controls. In conclusion, halopriming can be considered a useful tool to enhance salinity tolerance in beans and other salt-sensitive crops. Full article

Enteral nutrition (EN) therapy in ICU patients requiring oxygen therapy with high-flow nasal cannula (HFNC) and/or noninvasive mechanical ventilation (NIMV) is controversial. A prospective, cohort, observational, and multicenter study was conducted in 10 ICUs in Spain to analyze the 90-day mortality, tolerance, side effects, and infectious complications of trophic EN in patients requiring HFNC therapy and/or NIVM. A total of 149 patients were enrolled. The mean age, severity scores, tracheobronchitis, bacteremia, and antimicrobial therapy were significantly higher in deceased than in living patients (p < 0.05), and the mortality rate was 14.8%. A total of 110 patients received oral trophic feedings, 36 patients received nasogastric tube feedings (NGFs), and 3 received mixed feedings. Trophic EN was discontinued in only ten (14.9%) patients because of feeding-related complications. The variables selected for the multivariate logistic regression on feeding discontinuation were SOFA upon admission (OR per unit = 1.461) and urea (OR per mg/dL = 1.029). There were no significant differences in the development of new infections according to the route of EN administration. Early trophic feeding administered to patients with acute respiratory failure requiring noninvasive ventilation is safe and feasible, and is associated with few dietary and infectious complications in a mortality, setting comparable to similar studies. Full article

Bi₂S₃ has gained considerable attention as a semiconductor for its versatile functional properties, finding application across various fields, and liquid phase exfoliation (LPE) serves as a straightforward method to produce it in nano-form. Till now, the commonly used solvent for LPE has been N-Methyl-2-pyrrolidone, which is expensive, toxic and has a high boiling point. These limitations drive the search for more sustainable alternatives, with water being a promising option. Nonetheless, surfactants are necessary for LPE in water due to the hydrophobic nature of Bi₂S₃, and organic molecules with amphoteric characteristics are identified as suitable surfactants. However, systematic studies on the use of ionic surfactants in the LPE of Bi₂S₃ have remained scarce until now. In this work, we used sodium dodecyl sulfate (SDS), sodium dodecylbenzene sulfonate (SDBS) and sodium hexadecyl sulfonate (SHS) as representative species and we present a comprehensive investigation into their effects on the LPE of Bi₂S₃. Through characterizations of the resulting products, we find that all surfactants effectively exfoliate Bi₂S₃ into few-layer species. Notably, SDBS demonstrates superior stabilization of the 2D layers compared to the other surfactants, while SHS becomes the most promising surfactant for obtaining products with high yield. Moreover, the resulting nano-inks are used for fabricating films using spray-coating, reaching a fine tuning of band gap by controlling the number of cycles, and paving the way for the utilization of 2D Bi₂S₃ in optoelectronic devices. Full article

Three-dimensional (3D) integration has become a leading approach in chip packaging. The interconnection density and reliability of micro-bumps in chip stacking are often threatened by high bonding temperatures. The method of building chip-to-chip interconnections by electroless deposition of metal has its distinct merit, while the interfacial defect issue, especially that related to voiding during the merging of opposite sides, remains largely unsolved. In this study, to trace the influencing factors in the voiding, the growth characteristics of the electroless all-copper interconnections were examined by carrying out deposition experiments in a microfluidic channel device. The results show that when the gap between the opposite copper bumps to be electrolessly merged is as low as 10 μm, significant voids appear at the inflow side and the top of the copper bumps because the hydrogen cannot be expelled in time. A finite-element flow model of the plating solution between the chips was established, which showed that the flow rate of the plating solution around the copper bumps was much higher than in the merging gap, causing an uneven supply of reactants. Based on these findings, we proposed two potential solutions, one is to improve the flow mode of the plating solution, and the other is to add the reaction inhibitor, 2,2′-bipyridine. Finally, the combination of these two approaches successfully achieved an improved merging quality of the copper joints. Full article

Osteoarthritis is more prevalent than any other form of arthritis and is characterized by the progressive mechanical deterioration of joints. Glucosamine, an amino monosaccharide, has been used for over fifty years as a dietary supplement to alleviate osteoarthritis-related discomfort. Silibinin, extracted from milk thistle, modifies the degree of glycosylation of target proteins, making it an essential component in the treatment of various diseases. In this study, we aimed to investigate the functional roles of glucosamine and silibinin in cartilage homeostasis using the TC28a2 cell line. Western blots showed that glucosamine suppressed the N-glycosylation of the gp130, EGFR, and N-cadherin proteins. Furthermore, both glucosamine and silibinin differentially decreased and increased target proteins such as gp130, Snail, and KLF4 in TC28a2 cells. We observed that both compounds dose-dependently induced the proliferation of TC28a2 cells. Our MitoSOX and DCFH-DA dye data showed that 1 µM glucosamine suppressed mitochondrial reactive oxygen species (ROS) generation and induced cytosol ROS generation, whereas silibinin induced both mitochondrial and cytosol ROS generation in TC28a2 cells. Our JC-1 data showed that glucosamine increased red aggregates, resulting in an increase in the red/green fluorescence intensity ratio, while all the tested silibinin concentrations increased the green monomers, resulting in decreases in the red/green ratio. We observed increasing subG1 and S populations and decreasing G1 and G2/M populations with increasing amounts of glucosamine, while increasing amounts of silibinin led to increases in subG1, S, and G2/M populations and decreases in G1 populations in TC28a2 cells. MTT data showed that both glucosamine and silibinin induced cytotoxicity in TC28a2 cells in a dose-dependent manner. Regarding endoplasmic reticulum stress, both compounds induced the expression of CHOP and increased the level of p-eIF2α/eIF2α. With respect to O-GlcNAcylation status, glucosamine and silibinin both reduced the levels of O-GlcNAc transferase and hypoxia-inducible factor 1 alpha. Furthermore, we examined proteins and mRNAs related to these processes. In summary, our findings demonstrated that these compounds differentially modulated cellular proliferation, mitochondrial and cytosol ROS generation, the mitochondrial membrane potential, the cell cycle profile, and autophagy. Therefore, we conclude that glucosamine and silibinin not only mediate glycosylation modifications but also regulate cellular processes in human chondrocytes. Full article

Typhoon-induced slope failure is one of the most important geological hazards in coastal areas. However, the specific influence of typhoons on the stability of residual soil slopes still remains an open issue. In this study, the Feiyunjiang catchment in Zhejiang Province of SE China was chosen as the study area, and a downscaling physical model of residual soil slopes in the region was used to carry out the wind tunnel test. Our aim was to answer the question, How does the vegetation on the slope and slope stability respond during a typhoon event? For this purpose, multiple aspects were monitored and observed under four different wind speeds (8.3 m/s, 10.3 m/s, 13.3 m/s, and 17 m/s), including vegetation damage on the slope, macrocracks on the slope surface, wind pressure, wind load, permeability coefficient of the soil layer, and slope stability. The results showed that the plants on the slope could restore to their original states when the wind speeds ranged from 8.3 m/s to 13.3 m/s, but were damaged to the point of toppling when the wind speed increased to 17 m/s. Meanwhile, evident cracks were observed on the ground under this condition, which caused a sharp increase in the soil permeability coefficient, from 1.06 × 10⁻⁵ m/s to 6.06 × 10⁻⁴ m/s. The monitored wind pressures were larger at the canopy than that at the trunk for most of the trees, and generally larger at the crown of the slope compared with the toe of the slope. Regarding the wind load to the slope ground, the total value increased significantly, from 35.4 N under a wind speed of 8.3 m/s to 166.5 N under a wind speed of 17 m/s. However, the wind load presented different vector directions at different sections of the slope. The quantitative assessment of slope stability considering the wind load effect revealed that the safety factor decreased by 0.123 and 0.1 under the natural state and saturated state, respectively, from no wind to a 17 m/s strong wind. Overall, the present results explained the mechanism of slope failure during typhoon events, which provided theoretical reference for revealing the characteristics of residual soil slope stability under typhoon conditions. Full article

High-risk human papillomavirus (HR-HPV; HPV-16) and cigarette smoking are associated with cervical cancer (CC); however, the underlying mechanism(s) remain unclear. Additionally, the carcinogenic components of tobacco have been found in the cervical mucus of women smokers. Here, we determined the effects of cigarette smoke condensate (CSC; 3R4F) on human ectocervical cells (HPV-16 Ect/E6E7) exposed to CSC at various concentrations (10⁻⁶–100 μg/mL). We found CSC (10⁻³ or 10 μg/mL)-induced proliferation, enhanced migration, and histologic and electron microscopic changes consistent with EMT in ectocervical cells with a significant reduction in E-cadherin and an increase in the vimentin expression compared to controls at 72 h. There was increased phosphorylation of receptor tyrosine kinases (RTKs), including Eph receptors, FGFR, PDGFRA/B, and DDR2, with downstream Ras/MAPK/ERK1/2 activation and upregulation of common EMT-related genes, TGFB SNAI2, PDGFRB, and SMAD2. Our study demonstrated that CSC induces EMT in ectocervical cells with the upregulation of EMT-related genes, expression of protein biomarkers, and activation of RTKs that regulate TGFB expression, and other EMT-related genes. Understanding the molecular pathways and environmental factors that initiate EMT in ectocervical cells will help delineate molecular targets for intervention and define the role of EMT in the initiation and progression of cervical intraepithelial neoplasia and CC. Full article

There is a lack of scientific analysis and control over the production of date vinegar in Oman, despite its growing demand in the worldwide market. Traditional production of date vinegar may lead to elevated amounts of ethanol (≥0.5%) and reduced content of acetic acid (<4%) compared to the standard acceptable levels. This study aimed to isolate non-Gluconobacter species from date vinegar produced by spontaneous fermentation and formulate starter cultures for quick and efficient production of date vinegar. In spontaneous fermentation date vinegar samples, the highest concentration of acetic acid was 10.42% on day 50. Acetobacter malorum (5 isolates), A. persici (3 isolates), and A. tropicalis (3 isolates) were identified based on 16S rRNA gene sequences for the first time in date vinegar. For date vinegar prepared with a starter culture of Acetobacter and yeast, the highest concentration of acetic acid was 4.67%. In conclusion, spontaneous fermentation resulted in the production of date vinegar with a high concentration of acetic acid, acceptable concentrations of ethanol and methanol, and the first isolation of three Acetobacter species. The formulated starter culture produced acceptable amounts of acetic acid and the time of fermentation was reduced 10 times (from 40 days to 4 days). This can provide the basis for producing a personalized or commercial product that ensures the production of good-quality date vinegar in an easier, faster, safer, and more efficient way from low-quality and surplus dates. Full article

Hydrogen sulfide (H₂S), previously regarded as a toxic exhaust and atmospheric pollutant, has emerged as the third gaseous signaling molecule following nitric oxide (NO) and carbon monoxide (CO). Recent research has revealed significant biological effects of H₂S in a variety of systems, such as the nervous, cardiovascular, and digestive systems. Additionally, H₂S has been found to impact reproductive system function and may have therapeutic implications for reproductive disorders. This paper explores the relationship between H₂S and male reproductive disorders, specifically erectile dysfunction, prostate cancer, male infertility, and testicular damage. Additionally, it examines the impact of H₂S regulation on the pathophysiology of the female reproductive system, including improvements in preterm birth, endometriosis, pre-eclampsia, fetal growth restriction, unexplained recurrent spontaneous abortion, placental oxidative damage, embryo implantation, recovery of myometrium post-delivery, and ovulation. The study delves into the regulatory functions of H₂S within the reproductive systems of both genders, including its impact on the NO/cGMP pathway, the activation of K⁺ channels, and the relaxation mechanism of the spongy smooth muscle through the ROCK pathway, aiming to broaden the scope of potential therapeutic strategies for treating reproductive system disorders in clinical settings. Full article