Hematopoietic stem cell transplantation (HSCT) remains a cornerstone in the management of patients with hematological malignancies. Endothelial injury syndromes, such as HSCT-associated thrombotic microangiopathy (HSCT-TMA), veno-occlusive disease/sinusoidal obstruction syndrome (SOS/VOD), and capillary leak syndrome (CLS), constitute complications after HSCT. Moreover, endothelial damage is prevalent after immunotherapy with chimeric antigen receptor-T (CAR-T) and can be manifested with cytokine release syndrome (CRS) or immune effector cell-associated neurotoxicity syndrome (ICANS). Our literature review aims to investigate the genetic susceptibility in endothelial injury syndromes after HSCT and CAR-T cell therapy. Variations in complement pathway- and endothelial function-related genes have been associated with the development of HSCT-TMA. In these genes, CFHR5, CFHR1, CFHR3, CFI, ADAMTS13, CFB, C3, C4, C5, and MASP1 are included. Thus, patients with these variations might have a predisposition to complement activation, which is also exaggerated by other factors (such as acute graft-versus-host disease, infections, and calcineurin inhibitors). Few studies have examined the genetic susceptibility to SOS/VOD syndrome, and the implicated genes include CFH, methylenetetrahydrofolate reductase, and heparinase. Finally, specific mutations have been associated with the onset of CRS (PFKFB4, CX3CR1) and ICANS (PPM1D, DNMT3A, TE2, ASXL1). More research is essential in this field to achieve better outcomes for our patients. Full article

The vertical transition zone of mountain vegetation is characterized by high species diversity, and the width of the transition zone may serve as an indirect indicator of climate change. However, research into the differential characteristics of vegetation response to climate changes at the boundary of vertical transition zones has been limited. This study employs MODIS and climate data spanning 2001 to 2018 to investigate spatiotemporal trends in precipitation (PRE), temperature (TMP), radiation (RAD), and Normalized Difference Vegetation Index (NDVI) across nine montane deciduous broad-leaved forests in the eastern monsoon region of China. It explores the time-lag and -accumulation effects of climatic variables on NDVI, quantifying their relative contributions to both its short-term and interannual variations. Results show that, notably, with the Qinling-Daba Mountains as a demarcation, northern regions exhibit significant increases in RAD (0.874–2.047 W m⁻²/a), whereas southern regions demonstrate notable rises in TMP (0.59–0.73 °C/10a). Areas of lower annual PRE correspond to the most rapid increases in annual average NDVI (5.045 × 10⁻³/a). NDVI’s lag time and cumulative duration responses to TMP are the shortest (0 and 2~4 periods), while its correlation with RAD is the strongest (0.815–0.975), generally decreasing from higher to lower latitudes. TMP significantly affects NDVI variations, impacting both short-term and interannual trends, with PRE driving short-term fluctuations and RAD dictating long-term shifts. This research provides critical data and a theoretical framework that enhances our understanding of how regional vegetation’s vertical zonation responds to climate change, thereby making a substantial contribution to the study of mountain vegetation’s diverse adaptability to climatic variations. Full article

Conventional path-planning strategies for GMA-WAAM may encounter challenges related to geometrical features when printing complex-shaped builds. One alternative to mitigate geometry-related flaws is to use algorithms that optimise trajectory choices—for instance, using heuristics to find the most efficient trajectory. The algorithm can assess several trajectory strategies, such as contour, zigzag, raster, and even space-filling, to search for the best strategy according to the case. However, handling complex geometries by this means poses computational efficiency concerns. This research aimed to explore the potential of machine learning techniques as a solution to increase the computational efficiency of such algorithms. First, reinforcement learning (RL) concepts are introduced and compared with supervised machining learning concepts. The Multi-Armed Bandit (MAB) problem is explained and justified as a choice within the RL techniques. As a case study, a space-filling strategy was chosen to have this machining learning optimisation artifice in its algorithm for GMA-AM printing. Computational and experimental validations were conducted, demonstrating that adding MAB in the algorithm helped to achieve shorter trajectories, using fewer iterations than the original algorithm, potentially reducing printing time. These findings position the RL techniques, particularly MAB, as a promising machining learning solution to address setbacks in the space-filling strategy applied. Full article

The rise in outdoor temperatures and heatwaves highlights the limitations of buildings in adapting to warming conditions, even in temperate climates. This paper analyses the indoor overheating of residential dwellings in Pamplona (a city in northern Spain, with a Cfb climate) using an urban-scale diagnostic methodology and presents different envelopes’ retrofitting scenarios as a strategy to reduce it. The results come from energy simulations conducted during an extremely warm summer in 2022, considering the microclimate effects. The residential typologies most vulnerable to overheating are those with only one orientation, built before the EPBD 2002, and located on top floors. These dwellings show a 23.7% mean of indoor overheating hours (IOH), representing approximately 870 h above the EN 16798-1:2019 adaptive threshold from May to September. Renovating building envelopes to meet current energy standards reduces the IOH by an average of 8.6% and up to 15.35% in the most vulnerable typologies. In the retrofitting scenario with green roof systems, indoor temperatures are up to 0.5 °C lower than when roofs are renovated with traditional systems. This study assists policy-makers in preventing the risk of overheating within cities and encourages them to promote nature-based solutions in order to adapt urban residential buildings and cities to warming conditions. Full article

Benzoxazinoids (BXs) are tryptophan-derived indole metabolites and play a role in various physiological processes, such as auxin metabolism. Auxin is essential in the process of somatic embryogenesis (SE) in plants. In this study, we used bioinformatics, transcriptome data, exogenous treatment experiments, and qPCR analysis to study the evolutionary pattern of Bx genes in green plants, the regulatory mechanism of DlBx genes during early SE, and the effect of 2,4-dihydroxy-7-methoxy-1,4-benzoxazine-3-one (DIMBOA) on the early SE in Dimocarpus longan Lour. The results showed that 27 putative DlBxs were identified in the longan genome; the Bx genes evolved independently in monocots and dicots, and the main way of gene duplication for the DlBx was tandem duplication (TD) and the DlBx were strongly constrained by purification selection during evolution. The transcriptome data indicated varying expression levels of DlBx during longan early SE, and most DlBxs responded to light, temperature, drought stress, and 2,4-dichlorophenoxyacetic acid (2,4-D) treatment; qRT-PCR results showed DlBx1, DlBx6g and DlBx6h were responsive to auxin, and treatment with 0.1mg/L DIMBOA for 9 days significantly upregulated the expression levels of DlBx1, DlBx3g, DlBx6c, DlBx6f, DlB6h, DlBx7d, DlBx8, and DlBx9b. The correlation analysis showed a significantly negative correlation between the expression level of DlBx1 and the endogenous IAA contents; DIMBOA significantly promoted the early SE and significantly changed the endogenous IAA content, and the IAA content increased significantly at the 9th day and decreased significantly at the 13th day. Therefore, the results suggested that DIMBOA indirectly promote the early SE by changing the endogenous IAA content via affecting the expression level of DlBx1 and hydrogen peroxide (H₂O₂) content in longan. Full article

Background: The aim of this study was to evaluate the short-term efficacy and safety of the Paul Glaucoma Implant (PGI) in pediatric eyes diagnosed with glaucoma following congenital cataract surgery (GFCS). Methods: A retrospective, single-center, descriptive study was conducted on consecutive children diagnosed with GFCS who underwent PGI implantation between July 2022 and November 2023 at the University Medical Center Mainz. The primary outcome measure was the reduction in IOP at the last follow-up visit. Results: Ten eyes of nine children were included in the study. The mean follow-up time was 7.70 ± 4.22 months (4.68–10.72 months). At the end of the study follow-up, the mean (95% CI) reduction in IOP was −14.8 ± 8.73 mmHg (−8.56 to −21.04 mmHg, p < 0.001). At the last follow-up, 30.0% (3/10) of patients achieved an IOP (intraocular pressure) of ≥6 and ≤21 mmHg with a reduction in IOP of ≥25% without treatment, while 90.0% (9/10) achieved this target IOP regardless of glaucoma medication treatment. The mean number of antiglaucoma medications was significantly reduced from 3.50 (IQR = 1) to 2.0 (IQR = 2, p = 0.01), and the visual acuity logMAR improved from 1.26 ± 0.62 to 1.03 ± 0.48 (p = 0.04). Only one eye experienced numerical hypotony (4 mmHg) without choroidal detachment or anterior chamber shallowing within the first 24 h. No other adverse events were observed during the follow-up period. Conclusions: PGI implantation significantly lowered IOP and the number of antiglaucoma eye drops with a favorable safety profile in children diagnosed with GFCS, thereby achieving a high rate of qualified surgical success in the short term. Full article

Even slight structural differences between phytocannabinoid isomers are usually enough to cause a change in their biological properties. In this study, we used in vitro CB1 agonism/antagonism assays to compare the receptor binding functionality of THCV (tetrahydrocannabivarin) and HHC (hexahydrocannabinol) isomers and applied molecular docking to provide an explanation for the difference in the activities. No CB1 agonism was observed for ∆9- and ∆8-THCV. Instead, both isomers antagonized CP 55940, with ∆9-THCV being approximately two times more potent than the ∆8 counterpart (IC₅₀ = 52.4 nM and 119.6 nM for ∆9- and ∆8-THCV, respectively). Docking simulations found two binding poses for THCV isomers, one very similar to ∆9-THC and one newly discovered pose involving the occupation of side pocket 1 of the CB1 receptor by the alkyl chain of the ligand. We suggested the latter as a potential antagonist pose. In addition, our results established 9R-HHC and 9S-HHC among partial agonists of the CB1 receptor. The 9R-HHC (EC₅₀ = 53.4 nM) isomer was a significantly more potent agonist than 9S (EC₅₀ = 624.3 nM). ∆9-THC and 9R-HHC showed comparable binding poses inside the receptor pocket, whereas 9S-HHC adopted a new and different binding posture that can explain its weak agonist activity. Full article

Background/Objectives: To assess free-breathing, dynamic radial magnetic resonance angiography (MRA) for detecting endoleaks post-endovascular aortic repair (EVAR) in cases with inconclusive computed tomography angiography (CTA). Methods: This prospective single-center study included 17 participants (mean age, 70 ± 9 years; 13 males) who underwent dynamic radial MRI (Golden-angle RAdial Sparse Parallel-Volumetric Interpolated BrEath-hold, GRASP-VIBE) after inconclusive multiphasic CT for the presence of endoleaks during the follow-up of EVAR-treated abdominal aortic aneurysms. CT and MRI datasets were independently assessed by two radiologists for image quality, diagnostic confidence, and the presence/type of endoleak. Statistical analyses included interrater and intermethod agreement, and diagnostic performance (sensitivity, specificity, area under the curve (AUC)). Results: Subjective image analysis demonstrated good image quality and interrater agreement (k ≥ 0.6) for both modalities, while diagnostic confidence was significantly higher in MRA (p = 0.03). There was significantly improved accuracy for detecting type II endoleaks on MRA (AUC 0.97 [95% CI: 0.87, 1.0]) compared to CTA (AUC 0.66 [95% CI: 0.41, 0.91]; p = 0.03). Although MRA demonstrated higher values for sensitivity, specificity, AUC, and interrater agreement, none of the other types nor the overall detection rate for endoleaks showed differences in the diagnostic performance over CT (p ≥ 0.12). CTA and MRA revealed slight to moderate intermethod concordance in endoleak detection (k = 0.3–0.64). Conclusions: The GRASP-VIBE MRA characterized by high spatial and temporal resolution demonstrates clinical feasibility with good image quality and superior diagnostic confidence. It notably enhances diagnostic performance in detecting and classifying endoleaks, particularly type II, compared to traditional multiphase CTA with inconclusive findings. Full article

With the flourishing development of corpus linguistics and technological revolutions in the AI-powered age, automated essay scoring (AES) models have been intensively developed. However, the intricate relationship between linguistic features and different constructs of writing quality has yet to be thoroughly investigated. The present study harnessed computational analytic tools and Principal Component Analysis (PCA) to distill and refine linguistic indicators for model construction. Findings revealed that both micro-features and their combination with aggregated features robustly described writing quality over aggregated features alone. Linear and non-linear models were thus developed to explore the associations between linguistic features and different constructs of writing quality. The non-linear AES model with Random Forest Regression demonstrated superior performance over other benchmark models. Furthermore, SHapley Additive exPlanations (SHAP) was employed to pinpoint the most powerful linguistic features for each rating trait, enhancing the model’s transparency through explainable AI (XAI). These insights hold the potential to substantially facilitate the advancement of multi-dimensional approaches toward writing assessment and instruction. Full article

Integrating wind power plants into the electricity grid poses challenges due to the intermittent nature of wind energy generation. Energy storage systems (ESSs) have shown promise in mitigating the intermittent variability associated with wind power. This paper presents a distributionally robust optimization (DRO) model for sizing energy storage systems to dispatch wind power plants. The variable wind power is formulated as a moment-based ambiguity set. Dispatchability is described by the expected value of the insufficient power of wind power relative to the dispatch command, which is a sum of nonlinear functions and is taken as the optimal index. A deterministic semi-definite positive model is derived to solve the problem effectively. Numerical studies are conducted to demonstrate the effectiveness and advantages of the proposed method. Full article

Unilateral condylar hyperplasia (UCH) is a rare cause of asymmetrical mandibular overgrowth because of the presence of an atypical growth in the affected condyle. SPECT (single-photon emission computed tomography) can easily establish the presence of an atypical, prolonged growth exceeding far beyond normal condylar growth and activity. A CT, CBCT, or LDCT (computed tomography, cone-beam computed tomography, or low-dose computed tomography) can confirm the diagnosis by evaluating the scope of bone overgrowth, mandibular basis/ramus asymmetry, tendency to condylar head enlargement, changes in bone density, and occurrence of differences in condylar head shapes, size, and bone structure. In most cases, a condylectomy is the procedure of choice in growing cases of UCH to remove the pathological condyle and reduce asymmetry levels. Sometimes, the growth is very slow and progressive over time, causing slowly growing asymmetry with similar symptoms to any other mandibular asymmetry, and this causes some troublesome procedures in UCH diagnostics, resulting in patients being underdiagnosed; it can even lead to some relapses in mandibular asymmetry and skeletal malocclusion after previously performed orthodontic and surgical treatment of such discrepancies. When the source of asymmetry is not identified in time, possible inadequate treatment protocols can be used. If any relapse of facial and mandibular asymmetry re-occur, SPECT and CT evaluation are necessary to evaluate if condylar hyperplasia is present and to establish what kind of surgical intervention should be used in each case. Full article

The Qaidam Block, located at the northern Qinghai–Tibet Plateau, is a pivotal area in unraveling the closure time of the Kunlun Ocean basin which might have recorded the transformation process between the Proto-Tethys and Paleo-Tethys Ocean basins. However, the late Triassic position of the Qaidam Block remains enigmatic, largely due to the scarcity of paleomagnetic data essential for quantitatively determining its paleolatitude. The widespread presence of the Elashan formation, particularly along the southern periphery of the Qaidam block, presents good material for conducting paleomagnetic work. Nevertheless, the primary magnetic carriers preserved within the Elashan formation might be influenced by multiple tectonic thermal events, particularly those associated with collisions between southern blocks and the Qaidam Block. Here we present rock magnetism and magnetic fabrics studies to identify the content and composition of magnetic minerals within the Elashan formation. The rock magnetic and petrologic results show that the magnetic carriers in the samples from the Elashan formation are dominated by magnetite with a small amount of goethite, pyrrhotite, and hematite. The results of Anisotropy in Magnetic Susceptibility indicate that the south of the Longwalangku section might not be obviously influenced by the tectonic events. Our results also provided guidance for future paleomagnetic research, emphasizing the importance of conducting further sampling away from adjacent faults, particularly in the southern Longwalangku area. Full article

Siderophores are a class of small molecules renowned for their high iron binding capacity, essential for all life forms requiring iron. This article provides a detailed review of the diverse classifications, and biosynthetic pathways of siderophores, with a particular emphasis on siderophores synthesized via nonribosomal peptide synthetase (NRPS) and non-NRPS pathways. We further explore the secretion mechanisms of siderophores in microbes and plants, and their role in regulating bioavailable iron levels. Beyond biological functions, the applications of siderophores in medicine, agriculture, and environmental sciences are extensively discussed. These applications include biological pest control, disease treatment, ecological pollution remediation, and heavy metal ion removal. Through a comprehensive analysis of the chemical properties and biological activities of siderophores, this paper demonstrates their wide prospects in scientific research and practical applications, while also highlighting current research gaps and potential future directions. Full article

Background: A negative lifestyle is reported to be related to cognitive problems. However, there is little information about this in relation to university students. The objective of the present study was to investigate the association between executive functions (EFs) and lifestyle parameters (i.e., physical activity (PA), sleep duration, screen time (ST), and food habits) among Chilean university students. Methods: This cross-sectional study included a total of 150 university students (94 females and 56 males, aged 21.28 ± 3.15 and 22.18 ± 2.90 years, respectively). Cognitive outcomes were measured using the CogniFit assessment battery. Lifestyle was measured through validated questionnaires. Results: Across the total sample, attention exhibited a positive association with PA h/week (β: 24.34 95% CI: 12.46 to 36.22, p = 0.001). Additionally, coordination was positively associated with PA h/week (β: 15.06 95% CI: 0.62 to 29.50, p < 0.041). PA h/week was positively linked with reasoning (β: 20.34 95% CI: 4.52 to 36.17, p = 0.012) and perception (β: 13.81 95% CI: 4.14 to 23.49, p = 0.005). Moreover, PA h/week was significantly linked to memory (β: 23.01 95% CI: 7.62 to 38.40, p = 0.004). In terms of the EFs, PA h/week showed a positive association with cognitive flexibility (β: 45.60 95% CI: 23.22 to 67.69, p = 0.001). Conclusions: In conclusion, lifestyle (PA h/week) was positively associated with EFs. Therefore, an increase in PA levels among these students should be a target for community- and university-based interventions in order to promote cognitive development such as attention, coordination, reasoning, perception, memory, and cognitive flexibility. Full article

The emergence of combination antiretroviral therapy (cART) has greatly transformed the life expectancy of people living with HIV (PWH). Today, over 76% of the individuals with HIV have access to this life-saving therapy. However, this progress has come with a new challenge: an increase in age-related non-AIDS conditions among patients with HIV. These conditions manifest earlier in PWH than in uninfected individuals, accelerating the aging process. Like PWH, the uninfected aging population experiences immunosenescence marked by an increased proinflammatory environment. This phenomenon is linked to chronic inflammation, driven in part by cellular structures called inflammasomes. Inflammatory signaling pathways activated by HIV-1 infection play a key role in inflammasome formation, suggesting a crucial link between HIV and a chronic inflammatory state. This review outlines the inflammatory processes triggered by HIV-1 infection and aging, with a focus on the inflammasomes. This review also explores current research regarding inflammasomes and potential strategies for targeting inflammasomes to mitigate inflammation. Further research on inflammasome signaling presents a unique opportunity to develop targeted interventions and innovative therapeutic modalities for combating HIV and aging-associated inflammatory processes. Full article

The Jerusalem artichoke (Helianthus tuberosus) is a high-yield crop, and a great source of fermentable sugars, which gives the plant the potential to be used as raw material for economical fuel alcohol production. In this article, the authors focus on the technological aspect of the biofuel manufacturing process and its properties. First, the fuel alcohol manufacturing process is described, afterwards assessing its characteristics such as kinematic viscosity, density and octane number. The amount of fuel alcohol obtained from 10 kg of biomass equals to 0.85 L. Afterwards, the mixtures of gasoline and obtained fuel alcohol are prepared and studied. Optimal alcohol and gasoline mixtures are determined to obtain biofuels with octane ratings of 92, 95 and 98. The kinematic viscosity of obtained mixtures does not differ significantly from its values for pure gasoline. The obtained biofuel mixture with 25% alcohol content yielded a decrease of sulfur content by 38%, an increase of vaporized fuel amount by 17.5% at 70 °C and by 10.5% at a temperature of 100 °C, which improves engine startup time and ensures its stable operation in comparison to pure gasoline. The alcohol obtained can be successfully used as a high-octane additive for gasolines. Full article

Multiple research directions have been proposed to study the information structure of Reddit. One of them is to model inter-subreddit relations but modeling user interactions in the form of a graph. Building upon prior work centered on political subreddits using pre-2020 data, we expand this investigation to include a more extensive dataset spanning 2022 and encompassing diverse topic areas. Employing NLP techniques such as text embeddings, we model subreddit content directly and construct a subreddit graph network based on cosine similarity. Community detection using the Louvain method reveals distinct subreddits and allows the analysis of inter-community connections via previous works’ concepts of “bridges” and “gateways”. Surprisingly, our findings indicate redundancy between bridges and gateways in the utilized dataset. Therefore, we introduce a new concept, “highways”. Highways, representing the most traversed paths between subreddits, unveil insights not captured by previous analyses, underscoring the significance of novel conceptual frameworks in uncovering latent knowledge within Reddit’s online community structures. Full article

This study aimed to compare the impact of CAD/CAM closed systems and open systems on the marginal gap of monolithic zirconia-reinforced lithium silicate (ZLS) ceramic crowns, as both systems are used in everyday dentistry, both chair-side and laboratory. For the closed system, 20 plastic teeth were scanned by a Primescan intra-oral scanner (IOS), and for the open system, the same number of plastic teeth were scanned by Trios 4 IOS. For the closed system, CEREC software was used, and for the open system, EXOCAD software was used. All 40 ZLS crowns were grinded by the same four-axis machine and cemented with Temp-bond, followed by self-adhesive resin cement. For each type of cement, an evaluation of the marginal gap was conducted by scanning electron microscopy (SEM). Before comparisons between the groups, a Kolmogorov–Smirnov test was performed on the study variables showing a normal distribution (p > 0.05). Independent T tests (α = 0.05) and paired-sample T tests (α = 0.05) were used. The independent T test found no significant mean marginal gap differences in the zirconia-reinforced lithium silicate crowns bonded with Temp-bond and scanned by Primescan (28.09 μm ± 3.06) compared to Trios 4 (28.94 μm ± 3.30) (p = 0.401), and there was no significant mean marginal gap differences in zirconia-reinforced lithium silicate crowns bonded with self-adhesive resin cement (Gcem ONE) and scanned by Primescan (46.70 μm ± 3.80) compared to Trios 4 (47.79 μm ± 2.59) (p = 0.295). Paired-sample T tests showed significantly higher mean marginal gaps with Gcem ONE compared to Temp-bond for the total mean marginal gap when scanning with Primescan (p = 0.0005) or Trios 4 (p = 0.0005). In everyday dentistry, both closed systems (Primescan with Cerec) and open systems (Trios 4 with Exocad) can be used to achieve an acceptable (<120 µm) marginal gap for ZLS CELTRA^® DUO single crowns. There is a significant difference between cementation with Temp-bond and Gcem ONE self-adhesive resin cement (p < 0.05). Full article

Amino acids are organic compounds that enter the protein structure, being involved in the proper functioning of the body. The role of amino acids in the onset of autism spectrum disorder (ASD) is yet to be established. Our aim was to identify correlations between urine amino acids and their derivatives and ASD. Methods: We designed a case–control study that consisted of 75 boys and girls, aged between 2 and 12 years. For amino acid profile, we used urine samples that were analyzed using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Results: Descriptive analysis showed higher values for glutamine, hydroxyproline, tyrosine, aspartic acid, and tryptophan and lower values for serine in the autism group than in the control group. Also, we found that boys with autism had higher values than the boys in the control group for serine, threonine, and aspartic acid. For girls from both groups, we did not find statistically significant values. In terms of age groups, we found significantly higher values for histidine, threonine, valine, methionine, aspartic acid, glutamic acid, alpha amino-adipic acid, sarcosine, alanine, and beta-alanine and significantly lower values for proline for both the autism and control groups under 5 years. Conclusions: The findings of this study support the assumption that amino acids may have a role in the expression of ASD. Full article

The sustainability of the food production achieved with the help of irrigation systems and the sustainability of their energy consumption are major challenges of the current century. Pumping systems currently account for approximately 30% of global electrical energy consumption. As electricity prices rise, there is a growing need for technological advancements to enhance energy efficiency and reduce consumption costs effectively. This study focuses on operating centrifugal pumps at variable frequency as an effective means of achieving this goal. Most centrifugal pump manufacturers/providers traditionally assume that pump efficiency remains constant across various operating frequencies, often equating the efficiency at various frequencies to that at the standard frequency (50/60 Hz). In contrast, this paper introduces a new formula for estimating pump efficiency, crucial to precise power consumption determination, particularly in variable-frequency scenarios. The formula parameters are identified by using experimental data acquired from an existing pumping system. The tests and results presented in this paper demonstrate that the proposed formula outperforms the formulas of the current industry standards in accuracy. Practically, the new relation assures enhanced accuracy in estimating pump efficiency and absorbed power, allowing for the design of a more precise model used for control systems synthesis required for operating centrifugal pumps at variable frequency. This research offers a new way of calculating pump efficiency, which could be very useful for industry practitioners seeking to optimize energy consumption in pumping systems. Full article

Crosstalk regulation is widespread in Streptomyces species. Elucidating the influence of a specific regulator on target biosynthetic gene clusters (BGCs) and cell metabolism is crucial for strain improvement through regulatory protein engineering. PteF and PteR are two regulators that control the biosynthesis of filipin, which competes for building blocks with avermectins in Streptomyces avermitilis. However, little is known about the effects of PteF and PteR on avermectin biosynthesis. In this study, we investigated their impact on avermectin biosynthesis and global cell metabolism. The deletion of pteF resulted in a 55.49% avermectin titer improvement, which was 23.08% higher than that observed from pteR deletion, suggesting that PteF plays a more significant role in regulating avermectin biosynthesis, while PteF hardly influences the transcription level of genes in avermectin and other polyketide BGCs. Transcriptome data revealed that PteF exhibited a global regulatory effect. Avermectin production enhancement could be attributed to the repression of the tricarboxylic acid cycle and fatty acid biosynthetic pathway, as well as the enhancement of pathways supplying acyl-CoA precursors. These findings provide new insights into the role of PteF on avermectin biosynthesis and cell metabolism, offering important clues for designing and building efficient metabolic pathways to develop high-yield avermectin-producing strains. Full article

The use of autonomous and unmanned systems continues to increase, with uses spanning from package delivery to simple automation of tasks and from factory usage to defense industries and agricultural applications. With the proliferation of unmanned systems comes the question of how to secure the command-and-control communication links among such devices and their operators. In this work, we look at the use of the Messaging Layer Security (MLS) protocol, designed to support long-lived continuous sessions and group communication with a high degree of security. We build out MAUI—an MLS API for UxS Integration that provides an interface for the secure exchange of data between a ScanEagle unmanned aerial vehicle (UAV) and an unmanned surface vehicle (USV) in a multi-domain ad-hoc network configuration, and experiment on system limits such as the ciphersuite set-up time and message handling rates. The experiments in this work were conducted in virtual and physical environments between the UAV, USV, and a controller device (all of different platforms). Our results demonstrate the viability of capitalizing on MLS’s capabilities to securely and efficiently transmit data for distributed communication among various unmanned system platforms. Full article