8-17 DNAzymes (8-17, 17E, Mg5, and 17EV1) are in vitro-selected catalytic DNA molecules that are capable of cleaving complementary RNAs. The conserved residues in their similar catalytic cores, together with the metal ions, were suggested to contribute to the catalytic reaction. Based on the contribution of the less conserved residues in the bulge loop residues (W¹², A¹⁵, A^15.0) and the internal stem, new catalytic cores of 8-17 DNAzymes were programmed. The internal stem CTC-GAG seems to be more favorable for the DNAzymes than CCG-GGC, while an extra W^12.0 led to a significant loss of activity of DNAzymes, which is contrary to the positive effect of A^15.0, by which a new active DNAzyme 17EM was derived. It conducts a faster reaction than 17E. It is most active in the presence of Pb²⁺, with the metal ion preference of Pb²⁺ >> Zn²⁺ > Mn²⁺ > Ca²⁺ ≈ Mg²⁺. In the Pb²⁺ and Zn²⁺-mediated reactions of 17EM and 17E, the same Na⁺- and pH dependence were also observed as what was observed for 17E and other 8-17 DNAzymes. Therefore, 17EM is another member of the 8-17 DNAzymes, and it could be applied as a potential biosensor for RNA and metal ions. Full article

The bubbling flow plays a key role in gas–liquid–solid fluidized beds. To understand the intrinsic fluidization behaviors at the discrete bubble and particle scale, coupled simulations with the volume of fluid model and the discrete element method are performed to investigate the effects of the gas inlet velocity, particle properties and two-orifice bubbling flow on particle fluidization. Three-dimensional simulations are carried out to accurately capture the dynamic changes in the bubble shape and trajectory. A bubbling flow with a closely packed bed is simulated to study the onset of particle fluidization. The obvious phenomena of particle fluidization are presented by both the experiment and simulation. Although an increasing gas inlet velocity promotes particle fluidization, the good fluidization of particles cannot be achieved solely by increasing the gas inlet velocity. When the channel is packed with more particles, the bubbles take a longer time to pass through the higher particle bed, and the bubbles grow larger in the bed. The increase in particle density also extends the time needed for the bubbles to escape from the bed, and it is more difficult to fluidize the particles with a larger density. Even if more particles are added into the channel, the percentage of suspended particles is not significantly changed. The percentage of suspended particles is not increased with a decrease in the particle diameter. The particle suspension is not significantly improved by the bubbling flow with two orifices, while the particle velocity is increased due to the more frequent bubble–particle collisions. The findings from this study will be beneficial in guiding the enhancement of particle fluidization in multiphase reactors. Full article

To address challenges related to the inadequate representation and inaccurate discrimination of pedestrian attributes, we propose a novel method for person re-identification, which leverages global feature learning and classification optimization. Specifically, this approach integrates a Normalization-based Channel Attention Module into the fundamental ResNet50 backbone, utilizing a scaling factor to prioritize and enhance key pedestrian feature information. Furthermore, dynamic activation functions are employed to adaptively modulate the parameters of ReLU based on the input convolutional feature maps, thereby bolstering the nonlinear expression capabilities of the network model. By incorporating Arcface loss into the cross-entropy loss, the supervised model is trained to learn pedestrian features that exhibit significant inter-class variance while maintaining tight intra-class coherence. The evaluation of the enhanced model on two popular datasets, Market1501 and DukeMTMC-ReID, reveals improvements in Rank-1 accuracy by 1.28% and 1.4%, respectively, along with corresponding gains in the mean average precision (mAP) of 1.93% and 1.84%. These findings indicate that the proposed model is capable of extracting more robust pedestrian features, enhancing feature discriminability, and ultimately achieving superior recognition accuracy. Full article

The binding ratio of palmitic acid (PA) at the sn-2 position of triacylglycerols in infant formulas is lower than that in breast milk, resulting in higher levels of fecal PA. Even if the ratio is increased to 40–50%, fecal PA levels in formula-fed infants remain higher than those in breast–fed infants. In Japan, infant formulas with 50% or more of PA bound to sn-2 (high sn-2 PA milk) are commercially available; however, their effects on PA excretion have not been investigated. Therefore, this observational study aimed to preliminarily evaluate whether the feeding volume of high sn-2 PA milk is significantly associated with fecal total/soaped PA levels in newborns. Infant formulas were classified as high (≥50% of PA bound to sn-2) or low sn-2 (<50%) PA milk. Associations between feeding volume of high or low sn-2 PA milk and fecal PA levels were evaluated using multiple regression analysis models. The results showed that the feeding volume of low sn-2 PA milk was positively associated with fecal total/soaped PA levels, while there was no significant association between those of high sn-2 PA milk and fecal total/soaped PA levels. Our preliminary study suggests that high sn-2 PA milk may reduce increased fecal PA levels in formula-fed newborns. Full article

A novel aromatic imine derivative, 2′-(1,4-phenylene)bis[4-[(4-methoxyphenyl)methylene]-5(4H)-oxazolone] (PBMBO), was designed and synthesized as a yellow colorant additive for green color filters used in image sensors. The optical and thermal properties of the newly developed material were evaluated both in solution and within color filter film conditions. PBMBO demonstrated a molar extinction coefficient of 2.24 × 10⁴ L/mol·cm in solution, surpassing that of the commercially employed yellow colorant MBIQO by a factor of 1.82. Color resist (CR) films incorporating PBMBO exhibited outstanding optical characteristics, displaying 0.03% transmittance at 435 nm, 99.3% transmittance at 530 nm, and a sharp slope within the 400 to 550 nm range. The decomposition temperature of PBMBO was 303 °C, indicating relatively superior thermal stability compared to MBIQO. Consequently, PBMBO emerges as a highly promising candidate for a yellow colorant additive in imaging sensor color filters, owing to its exceptional optical and thermal stability. Its potential applications are anticipated to extend across various fields of organic semiconductors. Full article

This paper presents an experimental study of the effect of the mass of dust retained on a fibrous filter bed operating singly and in a “cyclone-filter-bed” system on changes in filtration efficiency and accuracy, as well as the increase in flow resistance. The research was carried out using a novel and unprecedented method, determining the dust absorption coefficient km of the filter baffle under laboratory conditions. A filtration system built of a single cyclone and a cylindrical filter cartridge with an appropriately sized surface set behind it was studied. Conditions corresponding to the actual operating conditions of the air filter were maintained: dust concentration, filtration speed and dust extraction from the cyclone settling tank. The purpose of the research was to evaluate filter materials with different structures in terms of filtration efficiency and accuracy, as well as flow resistance. The study showed that the parameters of the structure of filter materials—permeability, grammage and thickness—affect the process of retaining dust particles. It was shown that the increase in the flow resistance of the filter bed has a higher intensity when dust grains of small sizes are directed at it, which is the case when the bed is operated behind a cyclone, which separates larger dust grains from the air. There is a reduction in the operating time of the filtration system due to the limitation of the permissible resistance ∆p_fdop, and the corresponding dust absorption k_m has a lower value. For a fixed value of the flow resistance, the dust absorption coefficient k_m2 of three different filtration baffles AC, B2, and B, working with a cyclone, take values 50–100% smaller than when working in a single-stage system. It has been shown that the “cyclone-filter baffle” unit, due to its greater dust separation capability, allows the filter cartridge to operate for a longer time until a certain flow resistance is reached. This allows the unit to operate longer at lower flow resistance without changing the filter cartridge, thus saving energy. The k_m values obtained during the tests, using the proposed original method, allow the selection of the filter bed for specific vehicle operating conditions by modelling its course. Full article

Pomelo peel is a natural substance with antibacterial properties. Its extraction process is simple, and the raw materials are abundant. Microcapsules were prepared using melamine resin as the wall material and pomelo peel flavonoids as the core material. The optimization of microcapsule preparation was explored by orthogonal and single-factor experiments. The findings indicated that the optimum process for the preparation of microencapsulation was a 0.12:1 mass ratio of core to wall material, 60 °C microencapsulation reaction temperature, 800 rpm microencapsulation reaction stirring speed, and 2% emulsifier concentration. On this basis, the microcapsules were applied to waterborne coatings at different levels, 0%, 3.0%, 6.0%, 9.0%, 12.0%, and 15.0%, respectively, to prepare paint films, and the properties of the paint films were tested and explored. The test showed that the microcapsules added to the waterborne paint film exhibited antibacterial activity while retaining good optical and mechanical properties. In comparison with Escherichia coli, the microcapsules had a greater antibacterial rate against Staphylococcus aureus. When the content of microcapsules was 6.0%, the general performance of the waterborne paint film was optimal. The antibacterial rate of the paint film against Staphylococcus aureus and Escherichia coli was 40.5% and 50.5%, respectively. The color difference was 3.28. The paint film had a certain elasticity area, the elongation at break was 10.8%, and the roughness was 1.75 μm. We successfully prepared microcapsules capable of improving the antibacterial performance of waterborne paint film, which expands the application field of waterborne coatings and provides a certain reference value for the antibacterial research of waterborne coatings. Full article

In the processing of emotions, the brain prepares and reacts in distinctive manners depending upon the negative or positive nuance of the emotion elicitors. Previous investigations showed that negative elicitors generally evoke more intense neural activities than positive and neutral ones, as reflected in the augmented amplitude of all sub-components of the event-related potentials (ERP) late posterior positivity (LPP) complex, while less is known about the emotion of disgust. The present study aimed to examine whether the LPP complex during the processing of disgust stimuli showed greater amplitude than other emotion elicitors with negative or positive valences, thus confirming it as a neural marker of disgust-related negativity bias at earlier or later stages. Thus, in the present study, we leveraged the ERP technique during the execution of an affective self-administered visual stimuli task to disentangle the neural contributions associated with images of positive, negative, disgust, or neutral images. Crucially, we showed that handling with disgust elicitors prompted the greatest neural activity and the highest delay during self-administration. Overall, we demonstrated progressive neural activities associated with the unpleasantness of the emotion elicitors and peculiar processing for disgust compared with all other emotions. Full article

Background: Endometriosis-associated ovarian cancer (EAOC) is a well-known type of cancer that arises from ovarian endometrioma (OE). OE contains iron-rich fluid in its cysts due to repeated hemorrhages in the ovaries. However, distinguishing between benign and malignant tumors can be challenging. We conducted a retrospective study on magnetic resonance (MR) relaxometry of cyst fluid to distinguish EAOC from OE and reported that this method showed good accuracy. The purpose of this study is to evaluate the accuracy of a non-invasive method in re-evaluating pre-surgical diagnosis of malignancy by a prospective multicenter cohort study. Methods: After the standard diagnosis process, the R2 values were obtained using a 3T system. Data on the patients were then collected through the Case Report Form (CRF). Between December 2018 and March 2023, six hospitals enrolled 109 patients. Out of these, 81 patients met the criteria required for the study. Results: The R2 values calculated using MR relaxometry showed good discriminating ability with a cut-off of 15.74 (sensitivity 80.6%, specificity 75.0%, AUC = 0.750, p < 0.001) when considering atypical or borderline tumors as EAOC. When atypical and borderline cases were grouped as OE, EAOC could be distinguished with a cut-off of 16.87 (sensitivity 87.0%, specificity 61.1%). Conclusions: MR relaxometry has proven to be an effective tool for discriminating EAOC from OE. Regular use of this method is expected to provide significant insights for clinical practice. Full article

A microwave-assisted synthesis of dialkyl and cyclic H-phosphonates via bis(2,2,2 trifluoroethyl) phosphonate (BTFEP) is described. This method enables the synthesis of various cyclic H-phosphonates and hetero-substituted dialkyl H-phosphonates by simple alcoholysis under non-inert and additive-free conditions. Short reaction times and the requirement for only stoichiometric amounts of alcohol render this method attractive for synthetic applications. Full article

This study offers a detailed analysis of building information modeling (BIM) policy and implementation across Europe, significantly contributing to the sector’s digital transformation. By collating data from governmental, academic, and industry sources, it identifies key trends and evaluates the effectiveness of BIM policies in advancing technology within construction. A systematic literature review and text mining across major databases revealed an increasing focus on sustainability, particularly “life cycle assessment” and “energy efficiency”, aligning with the Industry 5.0 initiative. The research shows that 35% of European countries have or plan to introduce BIM mandates, highlighting BIM’s crucial role in enhancing construction practices and influencing policy frameworks. Insights from this study are valuable for researchers, practitioners, and policymakers, guiding the adoption and operationalization of BIM and emphasizing the need for thorough market preparation, including funding, training, and standardization. Additionally, the study suggests a correlation between a country’s economic development and its propensity to enforce BIM mandates. Future research could explore regional policy variations and delve into the theoretical aspects of policy adoption and innovation diffusion to further understand BIM uptake dynamics. Full article

A review of the last 399 years (1624–2023) on fin whales (Balaenoptera physalus) in the Mediterranean Sea was conducted, based on an extensive compilation of records published in the scientific literature, technical reports, public databases, journals, and social media. A total of 10,716 sightings and 575 mortality events have been computed, analysed by semesters and mapped in order to compare the summer–winter seasons especially and their implications on migration–residence. Visual and acoustic detections, feedings, migrations, primary production areas (chlorophyll), threats and causes of death and their relations have been addressed, and a mini-review on heavy metals and pollutants has been carried out on fin whales in the Mediterranean Sea. Mortality events were most frequent between November and April, coinciding with the decreased sighting period. Ship strikes posed the greatest threat, peaking between May and October, when marine traffic tends to increase in the Mediterranean Sea. Two populations coexist in the Mediterranean Sea, one resident and the other migratory, the latter using the Strait of Gibraltar for its biannual movements. Two areas with a presence of calves (up to 7 m in length) between October and February were detected: one scattered in the northern Mediterranean and the Strait of Gibraltar and its surroundings. A critical zone for collisions has been established according to the results for fin whales in the Mediterranean Sea. Full article

Sports-related muscle injuries account for 10–55% of all injuries, which is a growing concern, especially given the aging world population. To evaluate the process of skeletal muscle injury and compare it with muscle lesions observed in humans, we developed a novel in vivo model in sheep. In this model, muscle injury was induced by an ultrasound-guided transverse biopsy at the myotendinous junction of the medial gastrocnemius muscle. Twelve male sheep were examined at 3, 7, 14, and 28 days post-injury. Histological, immunofluorescence, and MRI analyses indicate that our sheep model could resemble key human clinicopathological features. Statistically significant differences (p < 0.05) were observed in collagen I, dMHC, α-SMA, and CD68 immunohistochemical detection when comparing injured and healthy muscles. The injured gastrocnemius muscle exhibited elevated levels of type I collagen, infiltration of CD68(+) macrophages, angiogenesis, and the emergence of newly regenerated dMHC(+) myofibers, which persisted for up to 4 weeks post-injury. Similarly, the progression of muscle injury in the sheep model was assessed using advanced clinical 3 T MRI and compared with MRI scans from human patients. The data indicate that the sheep muscle injury model presents features similar to those observed in human skeletal muscle injuries. This makes it a valuable large animal model for studying muscle injuries and developing novel therapeutic strategies. Full article

Coagulation is a process affected by multiple variables, nonlinear mapping and multiple perturbations. In order to realize the precise dosage of flocculants, polyaluminum chloride (PAC) was taken as the research object to explore the effects of temperature, water turbidity, pH and COD_Mn on the dosage of PAC and coagulation effect. A response surface methodology (RSM) experiment was carried out based on a single-factor experiment. The turbidity, pH and dosage of a single parameter, as well as the interaction term and secondary term, all have significant influence on coagulation effect. The optimal reaction conditions were calculated using Design-Expert software: pH, 7.48; turbidity, 14.59 NTU; dosage, 24.01 mg/L; and the error between the experimental value and the predicted value, 4.08%. Establishing a model with residual turbidity as a consideration index can help to calculate the optimal dosage of PAC, which is conducive to a reasonable and accurate control of the dosage of PAC in the coagulation process, so as to achieve the goal of low turbidity of effluent and low production cost. Full article

Lightweight and high-strength insulation materials have important application prospects in the aerospace, metallurgical, and nuclear industries. In this study, a highly porous silica fiber reinforced phenolic resin matrix composite was prepared by vacuum impregnation and atmospheric drying using quartz fiber needled felt as reinforcement and anhydrous ethanol as a pore-making agent. The effects of curing agent content on the structure, composition, density, and thermal conductivity of the composite were studied. The mechanical properties of the composite in the xy direction and z direction were analyzed. The results showed that this process can also produce porous phenolic resin (PR) with a density as low as 0.291 g/cm³, where spherical phenolic resin particles are interconnected to form a porous network structure with a particle size of about 5.43 μm. The fiber-reinforced porous PR had low density (0.372~0.397 g/cm³) and low thermal conductivity (0.085~0.095 W/m·K). The spherical phenolic resin particles inside the composite were well combined with the fiber at the interface and uniformly distributed in the fiber lap network. The composite possessed enhanced mechanical properties with compressive strength of 3.5–5.1 MPa in the xy direction and appeared as gradual compaction rather than destruction as the strain reached 30% in the z direction. This research provides a lightweight and high-strength insulation material with a simple preparation process and excellent performance. Full article

Mild steel corrosion is a significant challenge in oil and gas exploitation. Inhibitors are frequently employed to minimize the corrosive impact on mild steel. Mixing corrosion inhibitors is an effective method in reducing the dosage of toxic compounds and expanding the potential applications of inhibitors in NaCl solutions. Herein, a mixed corrosion inhibitor composed of imidazoline (IM), sodium molybdate, and sodium dodecylbenzenesulfonate (SDBS) for mild steel in a 3.5 wt% NaCl solution are investigated by orthogonal experimental design and electrochemical measurement. The imidazoline compound was synthesized and identified using Fourier transform infrared (FTIR) spectroscopy. The inhibitory effect is improved by higher concentrations of sodium molybdate and is further enhanced with the addition of 10 mg/L of SDBS. The electrochemical impedance spectroscopy indicates that the combination of IM (100 mg/L), sodium molybdate (50 mg/L), and SDBS (100 mg/L) results in excellent performance with electrochemical impedance (1.8 kohm·cm²). The mild steel surfaces after electrochemical measurement were analyzed using scanning electron microscopy (SEM). The information can contribute to the development of corrosion inhibitors with high performance or to understand the influence of mixing inhibitors on corrosion processes of mild steels. Full article

Fibre metal laminates (FMLs) are the most interesting composite materials of the past decade. They possess the properties of both polymer composites and metallic alloys. However, there is a problem with corrosion when the outer layers are made of aluminium or magnesium. The electrochemical changes that occur during the corrosion process and the mechanisms associated with the corrosion phenomenon are still being investigated. Recently, self-healing phenomena have emerged as a useful approach to prevent corrosion. However, there is limited research on the combination of FMLs and self-healing layers. Therefore, the main purpose of this article is to evaluate the self-healing ability of a magnesium/PEO layer based on microcapsules in a corrosion environment. It was observed that the corrosion mechanism in magnesium alloys is very complex. However, the use of a barrier layer with PEO treatment and microcapsules yielded positive anti-corrosion results. The FML samples were subjected to a 6-week corrosion test, and the addition of microcapsules to the layers showed positive results. In contrast, the samples without microcapsules exhibited intergranular corrosion. In the future, comprehensive tests using self-healing microcapsules in FMLs could greatly enhance their anti-corrosion properties and improve the integrity of the structure. Full article

This review, complemented by empirical investigations, delves into the intricate world of industrial powders, examining their elastic properties through diverse methodologies. The study critically assesses Young’s modulus (E) across eight different powder samples from various industries, including joint filler, wheat flour, wheat starch, gluten, glass beads, and sericite. Employing a multidisciplinary approach, integrating uniaxial compression methodologies—both single and cyclic—with vibration techniques, has revealed surprising insights. Particularly notable is the relationship between porosity and Young’s modulus, linking loose powders to the compacts generated under compression methods. Depending on the porosity of the powder bed, Young’s modulus can vary from a few MPa (loose powder) to several GPa (tablet), following an exponential trend. The discussion emphasizes the necessity of integrating various techniques, with a specific focus on the consolidation state of the powder bed, to achieve a comprehensive understanding of bulk elasticity. This underscores the need for low-consolidation methodologies that align more closely with powder technologies and unit operations such as conveying, transport, storage, and feeding. In conclusion, the study suggests avenues for further research, highlighting the importance of exploring bulk elastic properties in loose packing conditions, their relation with flowability, alongside the significance of powder conditioning. Full article

The utility of transcranial sonography (TCS) remains unclarified for the auxiliary diagnosis of Parkinson’s disease (PD). We investigated iodine-123 metaiodobenzylguanidine (MIBG) and TCS during the examination and diagnosis of high-signal-intensity substantia nigra lesion (HSI-SNL) incidence in PD patients previously diagnosed with dopamine transporter scintigraphy (DAT). The subjects were 67 patients with definitively diagnosed PD after DAT evaluation. Patients with midbrain substantia nigra visible during TCS who previously underwent MIBG were analyzed. The SN+ group comprised patients with extensive pathological HSI-SNL of Okawa class III/IV observed during TCS. The MIBG+ group comprised patients with a heart-to-mediastinum ratio of ≤2.2 during MIBG. TCS was performed to divide patients into the SN+ and SN− groups, and patient characteristics and MIBG findings were compared between the groups. PD was definitively diagnosed in 67 patients, among whom midbrain was visualized during TCS in 43 (64.1%) patients and pathological HSI-SNL was observed in 24 (35.8%). The MIBG findings were normal in six patients (27.3%) with HSI-SNL, and abnormal in seven (63.6%) without HSI-SNL. No significant differences were noted by Okawa classification in clinical characteristics based on the presence or absence of HSI-SNL. Multiple patients with normal findings during MIBG may have HSI-SNL. Thus, confirmatory imaging of HSI-SNL with TCS may be useful for diagnosis. Full article

The traditional receiver employs scalar tracking loops, resulting in degraded navigation performance in weak signal and high dynamic scenarios. An innovative design of a vector tracking receiver based on nonlinear Kalman filter (KF) tracking loops is proposed in this paper, which combines the strengths of both vector tracking and KF-based tracking loops. First, a comprehensive description of the vector tracking receiver model is presented, and unscented Kalman filter (UKF) is applied to nonlinear tracking loop. Second, to enhance the stability and robustness of the KF tracking loop, we introduce square root filtering and an adaptive mechanism. The tracking loop based on square root UKF (SRUKF) can dynamically adjust its filtering parameters based on signal noise and feedback Doppler error. Finally, the proposed method is implemented on a software-defined receiver (SDR), and the field vehicle experiment demonstrates the superiority of this method over other tracking methods in complex dynamic environments. Full article

Gestational diabetes mellitus (GDM) is a hyperglycemic state that is typically diagnosed by an oral glucose tolerance test (OGTT), which is unpleasant, time-consuming, has low reproducibility, and results are tardy. The machine learning (ML) predictive models that have been proposed to improve GDM diagnosis are usually based on instrumental methods that take hours to produce a result. Near-infrared (NIR) spectroscopy is a simple, fast, and low-cost analytical technique that has never been assessed for the prediction of GDM. This study aims to develop ML predictive models for GDM based on NIR spectroscopy, and to evaluate their potential as early detection or alternative screening tools according to their predictive power and duration of analysis. Serum samples from the first trimester (before GDM diagnosis) and the second trimester (at the time of GDM diagnosis) of pregnancy were analyzed by NIR spectroscopy. Four spectral ranges were considered, and 80 mathematical pretreatments were tested for each. NIR data-based models were built with single- and multi-block ML techniques. Every model was subjected to double cross-validation. The best models for first and second trimester achieved areas under the receiver operating characteristic curve of 0.5768 ± 0.0635 and 0.8836 ± 0.0259, respectively. This is the first study reporting NIR-spectroscopy-based methods for the prediction of GDM. The developed methods allow for prediction of GDM from 10 µL of serum in only 32 min. They are simple, fast, and have a great potential for application in clinical practice, especially as alternative screening tools to the OGTT for GDM diagnosis. Full article

Emerging research links the endocannabinoid system to gut microbiota, influencing nociception, mood, and immunity, yet the molecular interactions remain unclear. This study focused on the effects of probiotics on ECS markers—cannabinoid receptor type 2 (CB2) and fatty acid amide hydrolase (FAAH)—in dancers, a group selected due to their high exposure to physical and psychological stress. In a double-blind, placebo-controlled trial (ClinicalTrials.gov NCT05567653), 15 dancers were assigned to receive either a 12-week regimen of Lactobacillus helveticus Rosell-52 and Bifidobacterium longum Rosell-17 or a placebo (PLA: n = 10, PRO: n = 5). There were no significant changes in CB2 (probiotic: 0.55 to 0.29 ng/mL; placebo: 0.86 to 0.72 ng/mL) or FAAH levels (probiotic: 5.93 to 6.02 ng/mL; placebo: 6.46 to 6.94 ng/mL; p > 0.05). A trend toward improved sleep quality was observed in the probiotic group, while the placebo group showed a decline (PRO: from 1.4 to 1.0; PLA: from 0.8 to 1.2; p = 0.07841). No other differences were noted in assessed outcomes (pain and fatigue). Probiotic supplementation showed no significant impact on CB2 or FAAH levels, pain, or fatigue but suggested potential benefits for sleep quality, suggesting an area for further research. Full article