The cellulose present in the cell wall of vegetables prevents the greater release of nutrients to the animal. Therefore, the use of the cellulase enzyme is a viable strategy as it is capable of breaking cellulose bonds, releasing nutrients such as glucose, increasing dietary energy, and thus improving the productive performance of birds. Trichoderma reesei is efficient in the production of cellulase, which is produced via submerged fermentation followed by purification, formulation, and drying. Therefore, an experiment was carried out using 240 male broilers of the Cobb-500^® lineage to verify the effects resulting from the addition of powdered (500 g/t and 1000 g/t) and liquid (500 mL/t) cellulase over a period of 1 to 21 days. A completely randomized experimental design was used, consisting of four treatments with six replications and ten birds per replication that were housed in an experimental cage. It was observed that performance and digestibility results were significantly different with cellulase supplementation. Also, the relative weight of the large intestine in the period between one and seven days increased when cellulase was added at 1000 g/t. In the period of between eight and 14 days of life, the birds that consumed only the basal diet obtained higher levels of liver protein than those that received the treatments with the addition of the enzyme. However, 15 and 21 days, the consumed feed effect did not occur between thus, it is not conclusive whether hepatotoxicity occurs with the addition of cellulase. For the blood parameters, at 21 days, the diets with added cellulase were not significantly different regarding electrolytes. It was concluded that this cellulase produced by Trichoderma reesei can be included in the animals’ diet. Full article

This study is an in-depth exploration of the nascent field of Natural Language Processing (NLP) and generative Artificial Intelligence (AI), and it concentrates on the vital task of distinguishing between human-generated text and content that has been produced by AI models. Particularly, this research pioneers the identification of financial text derived from AI models such as ChatGPT and paraphrasing tools like QuillBot. While our primary focus is on financial content, we have also pinpointed texts generated by paragraph rewriting tools and utilized ChatGPT for various contexts this multiclass identification was missing in previous studies. In this paper, we use a comprehensive feature extraction methodology that combines TF–IDF with Word2Vec, along with individual feature extraction methods. Importantly, combining a Random Forest model with Word2Vec results in impressive outcomes. Moreover, this study investigates the significance of the window size parameters in the Word2Vec approach, revealing that a window size of one produces outstanding scores across various metrics, including accuracy, precision, recall and the F1 measure, all reaching a notable value of 0.74. In addition to this, our developed model performs well in classification, attaining AUC values of 0.94 for the ‘GPT’ class; 0.77 for the ‘Quil’ class; and 0.89 for the ‘Real’ class. We also achieved an accuracy of 0.72, precision of 0.71, recall of 0.72, and F1 of 0.71 for our extended prepared dataset. This study contributes significantly to the evolving landscape of AI text identification, providing valuable insights and promising directions for future research. Full article

The co-administration of curcumin and hesperetin might be beneficial in terms of neuroprotective activity; therefore, in this study, we attempted to develop a fixed-dose formulation comprising these two compounds in an amorphous state. The aim of obtaining an amorphous state was to overcome the limitations of the low solubility of the active compounds. First, we assessed the possibility of using popular sweeteners (erythritol, xylitol, and sorbitol) as plasticizers to reduce the glass transition temperature of PVP K30 to prepare the polymer–excipient blends, which allowed the preparation of amorphous solid dispersions via hot-melt extrusion at a temperature below the original glass transition of PVP K30. Erythritol proved to be the superior plasticizer. Then, we focused on the development of fixed-dose amorphous solid dispersions of curcumin and hesperetin. Powder X-ray diffraction and thermal analysis confirmed the amorphous character of dispersions, whereas infrared spectroscopy helped to assess the presence of intermolecular interactions. The amorphous state of the produced dispersions was maintained for 6 months, as shown in a stability study. Pharmaceutical parameters such as dissolution rate, solubility, and in vitro permeability through artificial membranes were evaluated. The best improvement in these features was noted for the dispersion, which contained 15% of the total content of the active compounds with erythritol used as the plasticizer. Full article

Alnus riparian communities are important for ecological stability. Data on Alnus species were gathered from herbaria and literature, revealing that 54.39% of the information refers to Alnus glutinosa, 39.42% to Alnus incana, and 6.18% to Alnus pubescens. This information highlights the widespread occurrence of A. glutinosa and A. incana, as described in distribution maps, contrasting with the more limited range of the hybrid A. pubescens. Principal component analysis (PCA), utilizing standardized factors, was carried out on 217 relevés of the communities of the studied species. In these relevés, we identified a total of 169 plant species, categorized as herbaceous (83%), trees (11%), and shrubs (6%). Three distinct forest communities, Stellario nemorum-Alnetum glutinosae, Alnetum incanae, and Telekio speciosae-Alnetum incanae, emerged from relevé groups. The distribution maps of the three taxa were overlaid on maps of the region’s protected areas, revealing a substantial presence of these taxa within the protected zones. The research aims to highlight the sustainability and conservation importance of Alnus communities in the Romanian Carpathians, to contribute to ongoing conservation efforts and promote the viability and resilience of these ecologically important wetland habitats. Full article

Renewable energy sources (RES) will play a key role in the transition to clean energy. Financial and socio-economic benefits determine the investment management in these energy sources. This article aims to indicate current energy policy goals, present socio-economic benefits resulting from renewable energy investments, and review further development directions in Poland. The research was carried out using desk research, case studies, and literature review methods to provide a broader economic context for RES investments. The scope of the research included both the Polish and the European Union contexts. The authors examined the Polish objectives of investment in renewable energy contained in strategic, planning, and other legal documents compared to EU targets, reviewed possible investments in renewable energy, and indicated wind farms and photovoltaic investments as the most effective ones from the point of view of further development which aims to meet the EU’s goals by 2030. The authors also demonstrated a wide range of socio-economic benefits based on literature reviews, analysis of policy documents, and regulations regarding the energy sector, and examined a specific example of investment implementation and identified the ecosystem of beneficiaries and their benefits. Full article

Forest-dwelling residents frequently collect non-timber forest products (NTFPs) for various reasons, such as food, medicine, firewood, religious reasons, or handicrafts. This study examines the multiple benefits derived from NTFPs and how they contribute to the livelihoods of Ghanaian communities to alleviate poverty. Resources and services provided by NTFPs have an essential role in providing economic, nutritional, medicinal, cultural, and environmental benefits. This study examines the diverse range of NTFPs locals collect, such as chewing sticks, games, herbs, honey, leaves, mushrooms, pestles, raffia and palms, snails, and straws. In addition to serving different purposes, these NTFPs contribute to income generation, food security, health care, cultural practices, and the protection of the environment. A qualitative research approach was used in this study to collect data through semi-structured interviews and focus groups with members on multiple collections of NTFPs and their multiple benefits. We interviewed 732 residents regarding their multiple NTFPs and their potential to improve the livelihood of the locals. The data for the study were analysed using descriptive statistics. As a result of the survey, the frequency and percentages of responses were analysed for each indicator related to multiple values of NTFPs. Both males and females collected a list of NTFPs during the survey. Regarding NTFPs used for sale, 275 were reported, representing 37.57% of the total. In addition to emphasising the importance of sustainable management practices and equitable distribution of benefits, the study explores the multiple benefits of NTFPs for poverty alleviation in Ghanaian communities. It is essential to ensure the conservation of forest ecosystems and promote inclusive policies to harness the potential of NTFPs and maximise their positive impact on livelihoods. NTFPs provide multiple benefits for Ghanaian communities, including income, nutrition, healthcare, cultural preservation, and environmental sustainability. To promote sustainable development, it is imperative to understand the role of NTFPs and implement appropriate strategies. Full article

To meet the increased demand for home workouts owing to the COVID-19 pandemic, this study proposes a new approach to real-time exercise posture classification based on the convolutional neural network (CNN) in an ensemble learning system. By utilizing MediaPipe, the proposed system extracts the joint coordinates and angles of the human body, which the CNN uses to learn the complex patterns of various exercises. Additionally, this new approach enhances classification performance by combining predictions from multiple image frames using an ensemble learning method. Infinity AI’s Fitness Basic Dataset is employed for validation, and the experiments demonstrate high accuracy in classifying exercises such as arm raises, squats, and overhead presses. The proposed model demonstrated its ability to effectively classify exercise postures in real time, achieving high rates in accuracy (92.12%), precision (91.62%), recall (91.64%), and F1 score (91.58%). This indicates its potential application in personalized fitness recommendations and physical therapy services, showcasing the possibility for beneficial use in these fields. Full article

İzmit Bay, a 50 km long inlet at the eastern end of the Marmara Sea, is crucial for the region’s economy, culture, and marine ecosystem. The bay’s water circulation regulates nutrient distribution, stratification, sedimentation, oxygen levels, heat, and pollution levels. It is also influenced by meteorological events, such as short-term moderate to strong wind conditions. This study investigated the sensitivity of İzmit Bay Water Circulation to wind speed, direction, and duration using the MITgcm model with Orlanski boundary conditions and process-oriented modeling. The simulations showed that under weak forcing conditions, seawater temperature, salinity, and stratification do not significantly vary. However, strong forcing and wind speeds (statistically defined by percentiles of observation data) of 4.9 m/s (75%), 6.7 m/s (90%), and 10.1 m/s (99%) generate significant mesoscale and sub-mesoscale processes, depending on the direction. Westerly component winds cause downwelling at the eastern coastline, while easterly component winds bring sub-surface water to the surface. Strong winds from N, NE, and E sectors lead to the rise in lower-layer waters in the western basin, forcing them to overflow through the Hersek Delta sill into the central basin. Overall, severe wind events greater than 4.9 m/s (75%) significantly affect the bay’s hydrography by transforming the upper layer, with a decrease in temperature up to 5 °C and an increase in salinity up to 10 ppt. Full article

This study describes the conception, development, and growth of the Triage Cancer Conference hosted by Triage Cancer, a national nonprofit organization providing free legal and financial education to the cancer community. We conducted a retrospective analysis of post-conference participant surveys. Descriptive statistics were calculated for participant demographics, and acceptability, feasibility, and appropriateness were evaluated. From 2016–2021, 1239 participants attended the conference and completed post-conference surveys. Participants included social workers (33%), nurses (30%), and cancer patients/survivors (21%), with representation from over 48 states. Among those who reported race, 16% were Black, and 7% were Hispanic. For acceptability, more than 90% of participants felt that the conference content, instructors, and format were suitable and useful. For feasibility, more than 90% of participants felt that the material was useful, with 93–96% reporting that they were likely to share the information and 98% reporting that they would attend another triage cancer event. Appropriateness was also high, with >80–90% reporting that the sessions met the pre-defined objectives. Triage Cancer fills an important gap in mitigating financial toxicity, and formal evaluation of these programs allows us to build evidence of the role and impact of these existing resources. Future research should focus on adding validated patient-reported outcomes, longer-term follow-up, and ensuring inclusion and evaluation of outcome metrics among vulnerable populations. Full article

Global urbanization and increasing traffic volume have intensified traffic congestion throughout transportation infrastructure, particularly at toll plazas, highlighting the critical need to implement proactive transportation infrastructure solutions. Traditional toll plaza management approaches, often relying on manual interventions, suffer from inefficiencies that fail to adapt to dynamic traffic flow and are unable to produce preemptive control strategies, resulting in prolonged queues, extended travel times, and adverse environmental effects. This study proposes a proactive traffic control strategy using advanced technologies to combat toll plaza congestion and optimize traffic management. The approach involves deep learning convolutional neural network models (YOLOv7–Deep SORT) for vehicle counting and an extended short-term memory model for short-term arrival rate prediction. When projected arrival rates exceed a threshold, the strategy proactively activates variable speed limits (VSLs) and ramp metering (RM) strategies during peak hours. The novelty of this study lies in its predictive and adaptive capabilities, ensuring efficient traffic flow management. Validated through a case study at Ravi Toll Plaza Lahore using PTV VISSIMv7, the proposed method reduces queue length by 57% and vehicle delays by 47% while cutting fuel consumption and pollutant emissions by 28.4% and 34%, respectively. Additionally, by identifying the limitations of conventional approaches, this study presents a novel framework alongside the proposed strategy to bridge the gap between theory and practice, making it easier for toll plaza operators and transportation authorities to adopt and benefit from advanced traffic management techniques. Ultimately, this study underscores the importance of integrated and proactive traffic control strategies in enhancing traffic management, minimizing congestion, and fostering a more sustainable transportation system. Full article

Laser powder bed fusion (LPBF) is a prospective and promising technique of additive manufacturing of which there is a growing interest for the development and production of Fe-based bulk metallic glasses and amorphous–nanocrystalline composites. Many factors affect the quality and properties of the resulting material, and these factors are being actively investigated by many researchers, however, the factor of the inert gas atmosphere used in the process remains virtually unexplored for Fe-based metallic glasses and composites at this time. Here, we present the results of producing amorphous–nanocrystalline composites from amorphous Fe-based powder via LPBF using argon and helium atmospheres. The analysis of the microstructures and phase compositions demonstrated that using helium as an inert gas in the LPBF resulted in a nearly three-fold increase in the amorphization degree of the material. Additionally, it had a beneficial impact on phase composition and structure in a heat-affected zone. The received results may help to develop approaches to control and improve the structural-phase state of amorphous–nanocrystalline compositional materials obtained via LPBF. Full article

Southern African societies are presently beleaguered by manifold socio-economic, political, and environmental challenges. Ordinary people long for answers to questions about how to mitigate these challenges. Meanwhile, the Church mostly preaches the gospel and establishes and grows denominations across the world. Proclaiming the gospel in word is good; however, without demonstrating the gospel with transformational deeds, the Church remains Salvationist and partially missionary. Bearing in mind that the integral mission of the Church is advancing the gospel holistically, fractional mindfulness of the gospel, hearing the words without performing the corresponding deeds, is defacing its identity. Toda–, this situation is problematic and helpless, as society is in dire need of a wholesome Church that acts in accordance with its own faith and values and attends to the soul, the body, and all other facets of life. Much has been published about the integral mission of the Church, though little has been said about its role in social action. By qualitatively reviewing the literature and observing the Southern African context and some biblical examples, this paper finds the integral mission to be the predominant and comprehensive purpose of the existence of the Church. While the Church could be aware of its mandate, it should be reawakened so that it becomes a doer of the Word and stands out as the salt and light of the world by contributing to addressing the needs of society. Full article

This review systematically investigates the critical role of natural binding proteins (NBPs), encompassing DNA-, RNA-, carbohydrate-, fatty acid-, and chitin-binding proteins, in the realms of oncology and diagnostics. In an era where cancer continues to pose significant challenges to healthcare systems worldwide, the innovative exploration of NBPs offers a promising frontier for advancing both the diagnostic accuracy and therapeutic efficacy of cancer management strategies. This manuscript provides an in-depth examination of the unique mechanisms by which NBPs interact with specific molecular targets, highlighting their potential to revolutionize cancer diagnostics and therapy. Furthermore, it discusses the burgeoning research on aptamers, demonstrating their utility as ‘nucleic acid antibodies’ for targeted therapy and precision diagnostics. Despite the promising applications of NBPs and aptamers in enhancing early cancer detection and developing personalized treatment protocols, this review identifies a critical knowledge gap: the need for comprehensive studies to understand the diverse functionalities and therapeutic potentials of NBPs across different cancer types and diagnostic scenarios. By bridging this gap, this manuscript underscores the importance of NBPs and aptamers in paving the way for next-generation diagnostics and targeted cancer treatments. Full article

The present article introduces a strategy for controlling oxidation and reduction reactions within polymer electrolyte membrane (PEM) networks as a means of enhancing storage capacity through the complexation of dissociated lithium cations with multifunctional groups of the polymer network. Specifically, co-polymer networks based on polysulfide (PS) and polyoxide (PO) precursors, photo-cured in the presence of succinonitrile (SCN) and lithium bis(trifluoro methane sulfonyl imide) (LiTFSI) salt, exhibited ionic conductivity on the order of mid 10⁻⁴ S/cm at ambient temperature in the 30/35/35 (weight %) composition. Lithium titanate (LTO, Li₄Ti₅O₁₂) electrode was chosen as an anode (i.e., a potential source of Li ions) against lithium iron phosphate (LFP, LiFePO₄) cathode in conjunction with polysulfide-co-polyoxide dual polyelectrolyte networks to control viscosity for 3D printability on conformal surfaces of drone and aeronautic vehicles. It was found that the PS-co-PO dual network-based polymer electrolyte containing SCN plasticizer and LiTFSI salt exhibited extra storage capacity (i.e., specific capacity of 44 mAh/g) with the overall specific capacity of 170 mAh/g (i.e., for the combined LTO electrode and PEM) initially that stabilized at 153 mAh/g after 50th cycles with a reasonable capacity retention of over 90% and Coulombic efficiency of over 99%. Of particular interest is the observation of the improved electrochemical performance of the polysulfide-co-polyoxide electrolyte dual-network relative to that of the polyoxide electrolyte single-network. Full article

Characterizing the coseismic slip behaviors of earthquakes could offer a better understanding of regional crustal deformation and future seismic potential assessments. On 18 December 2023, an Mw 6.0 earthquake occurred on the Lajishan–Jishishan fault system (LJFS) in the northeastern Tibetan Plateau, causing serious damage and casualties. The seismogenic fault hosting this earthquake is not well constrained, as no surface rupture was identified in the field. To address this issue, in this study, we use Interferometric Synthetic Aperture Radar (InSAR) data to investigate the coseismic surface deformation of this earthquake and invert both ascending and descending line-of-sight observations to probe the seismogenic fault and its slip characteristics. The InSAR observations show up to ~6 cm surface uplift caused by the Jishishan earthquake, which is consistent with the thrust-dominated focal mechanism. A Bayesian-based dislocation modeling indicates that two fault models, with eastern and western dip orientations, could reasonably fit the InSAR observations. By calculating the coseismic Coulomb failure stress changes (∆CFS) induced by both fault models, we find that the east-dipping fault scenario could reasonably explain the aftershock distributions under the framework of stress triggering, while the west-dipping fault scenario produced a negative ∆CFS in the region of dense aftershocks. Integrating regional geological structures, we suggest that the seismogenic fault of the Jishishan earthquake, which strikes NNE with a dip of 56° to the east, may be either the Jishishan western margin fault or a secondary buried branch. The optimal finite-fault slip modeling shows that the coseismic slip was dominated by reverse slip and confined to a depth range between ~5 and 15 km. The released seismic moment is 1.61 × 10¹⁸ N·m, which is equivalent to an Mw 6.07 earthquake. While the Jishishan earthquake ruptured a fault segment of approximately 20 km, it only released a small part of the seismic moment that was accumulated along the 220 km long Lajishan–Jishishan fault system. The remaining segments of the Lajishan–Jishishan fault system still have the capability to generate moderate-to-large earthquakes in the future. Full article

This review describes a 50-year-long research study on the characteristics of Helianthus tuberosus L. tuber dormancy, its natural release and programmed cell death (PCD), as well as on the ability to change the PCD so as to return the tuber to a life program. The experimentation on the tuber over the years is due to its particular properties of being naturally deficient in polyamines (PAs) during dormancy and of immediately reacting to transplants by growing and synthesizing PAs. This review summarizes the research conducted in a unicum body. As in nature, the tuber tissue has to furnish its storage substances to grow vegetative buds, whereby its destiny is PCD. The review’s main objective concerns data on PCD, the link with free and conjugated PAs and their capacity to switch the destiny of the tuber from a program of death to one of new life. PCD reversibility is an important biological challenge that is verified here but not reported in other experimental models. Important aspects of PA features are their capacity to change the cell functions from storage to meristematic ones and their involvement in amitosis and differentiation. Other roles reported here have also been confirmed in other plants. PAs exert multiple diverse roles, suggesting that they are not simply growth substances, as also further described in other plants. Full article

Pathogenic variants in the Crumbs homolog 1 (CRB1) gene lead to severe, childhood-onset retinal degeneration leading to blindness in early adulthood. There are no approved therapies, and traditional adeno-associated viral vector-based gene therapy approaches are challenged by the existence of multiple CRB1 isoforms. Here, we describe three CRB1 variants, including a novel, previously unreported variant that led to retinal degeneration. We offer a CRISPR-Cas-mediated DNA base editing strategy as a potential future therapeutic approach. This study is a retrospective case series. Clinical and genetic assessments were performed, including deep phenotyping by retinal imaging. In silico analyses were used to predict the pathogenicity of the novel variant and to determine whether the variants are amenable to DNA base editing strategies. Case 1 was a 24-year-old male with cone–rod dystrophy and retinal thickening typical of CRB1 retinopathy. He had a relatively preserved central outer retinal structure and a best corrected visual acuity (BCVA) of 60 ETDRS letters in both eyes. Genetic testing revealed compound heterozygous variants in exon 9: c.2843G>A, p.(Cys948Tyr) and a novel variant, c.2833G>A, p.(Gly945Arg), which was predicted to likely be pathogenic by an in silico analysis. Cases 2 and 3 were two brothers, aged 20 and 24, who presented with severe cone–rod dystrophy and a significant disruption of the outer nuclear layers. The BCVA was reduced to hand movements in both eyes in Case 2 and to 42 ETDRS letters in both eyes in Case 3. Case 2 was also affected with marked cystoid macular lesions, which are common in CRB1 retinopathy, but responded well to treatment with oral acetazolamide. Genetic testing revealed two c.2234C>T, p.(Thr745Met) variants in both brothers. As G-to-A and C-to-T variants, all three variants are amenable to adenine base editors (ABEs) targeting the forward strand in the Case 1 variants and the reverse strand in Cases 2 and 3. Available PAM sites were detected for KKH-nSaCas9-ABE8e for the c.2843G>A variant, nSaCas9-ABE8e and KKH-nSaCas9-ABE8e for the c.2833G>A variant, and nSpCas9-ABE8e for the c.2234C>T variant. In this case series, we report three pathogenic CRB1 variants, including a novel c.2833G>A variant associated with early-onset cone–rod dystrophy. We highlight the severity and rapid progression of the disease and offer ABEs as a potential future therapeutic approach for this devastating blinding condition. Full article

Cancer is considered one of the leading causes of death in the 21st century. The intensive search for new anticancer drugs has been actively pursued by chemists and pharmacologists for decades, focusing either on the isolation of compounds with cytotoxic properties from plants or on screening thousands of synthetic molecules. Compounds that could potentially become candidates for new anticancer drugs must have the ability to inhibit proliferation and/or induce apoptosis in cancer cells without causing too much damage to normal cells. Some anticancer compounds were discovered by accident, others as a result of long-term research. In this review, we have presented a brief history of the development of the most important groups of anticancer drugs, pointing to the fact that they all have many side effects. Full article

The relationship between psychological stress, altered skin immunity, and autophagy-related genes (ATGs) is currently unclear. Psoriasis is a chronic skin inflammation of unclear etiology that is characterized by persistence and recurrence. Immune dysregulation and emotional disturbances are recognized as significant risk factors. Emerging clinical evidence suggests a possible connection between anxiety disorders, heightened immune system activation, and altered skin immunity, offering a fresh perspective on the initiation of psoriasis. The aim of this study was to explore the potential shared biological mechanisms underlying the comorbidity of psoriasis and anxiety disorders. Psoriasis and anxiety disorders data were obtained from the GEO database. A list of 3254 ATGs was obtained from the public database. Differentially expressed genes (DEGs) were obtained by taking the intersection of DEGs between psoriasis and anxiety disorder samples and the list of ATGs. Five machine learning algorithms used screening hub genes. The ROC curve was performed to evaluate diagnostic performance. Then, GSEA, immune infiltration analysis, and network analysis were carried out. The Seurat and Monocle algorithms were used to depict T-cell evolution. Cellchat was used to infer the signaling pathway between keratinocytes and immune cells. Four key hub genes were identified as diagnostic genes related to psoriasis autophagy. Enrichment analysis showed that these genes are indeed related to T cells, autophagy, and immune regulation, and have good diagnostic efficacy validated. Using single-cell RNA sequencing analysis, we expanded our understanding of key cellular participants, including inflammatory keratinocytes and their interactions with immune cells. We found that the CASP7 gene is involved in the T-cell development process, and correlated with γδ T cells, warranting further investigation. We found that anxiety disorders are related to increased autophagy regulation, immune dysregulation, and inflammatory response, and are reflected in the onset and exacerbation of skin inflammation. The hub gene is involved in the process of immune signaling and immune regulation. The CASP7 gene, which is related with the development and differentiation of T cells, deserves further study. Potential biomarkers between psoriasis and anxiety disorders were identified, which are expected to aid in the prediction of disease diagnosis and the development of personalized treatments. Full article

Lymphadenectomy represents a fundamental step in the staging and treatment of non-small cell lung cancer (NSCLC). To date, the extension of lymphadenectomy in early-stage NSCLC is a debated topic due to its possible complications. The detection of sentinel lymph nodes (SLNs) is a strategy that can improve the selection of patients in which a more extended lymphadenectomy is necessary. This pilot study aimed to refine lymph nodal staging in early-stage NSCLC patients who underwent robotic lung resection through the application of innovative intraoperative sentinel lymph node (SLN) identification and the pathological evaluation using one-step nucleic acid amplification (OSNA). Clinical N0 NSCLC patients planning to undergo robotic lung resection were selected. The day before surgery, all patients underwent radionuclide computed tomography (CT)-guided marking of the primary lung lesion and subsequently Single Photon Emission Computed Tomography (SPECT) to identify tracer migration and, consequently, the area with higher radioactivity. On the day of surgery, the lymph nodal radioactivity was detected intraoperatively using a gamma camera. SLN was defined as the lymph node with the highest numerical value of radioactivity. The OSNA amplification, detecting the mRNA of CK19, was used for the detection of nodal metastases in the lymph nodes, including SLN. From March to July 2021, a total of 8 patients (3 female; 5 male), with a mean age of 66 years (range 48–77), were enrolled in the study. No complications relating to the CT-guided marking or preoperative SPECT were found. An average of 5.3 lymph nodal stations were examined (range 2–8). N2 positivity was found in 3 out of 8 patients (37.5%). Consequently, pathological examination of lymph nodes with OSNA resulted in three upstages from the clinical IB stage to pathological IIIA stage. Moreover, in 1 patient (18%) with nodal upstaging, a positive node was intraoperatively identified as SLN. Comparing this protocol to the usual practice, no difference was found in terms of the operating time, conversion rate, and complication rate. Our preliminary experience suggests that sentinel lymph node detection, in association with the accurate pathological staging of cN0 patients achieved using OSNA, is safe and effective in the identification of metastasis, which is usually undetected by standard diagnostic methods. Full article

Acidic stress in beef cattle slaughtering abattoirs can induce the acid adaptation response of in-plant contaminated Salmonella. This may further lead to multiple resistance responses threatening public health. Therefore, the acid, heat, osmotic and antibiotic resistances of Salmonella typhimurium (ATCC14028) were evaluated after a 90 min adaption in a pH = 5.4 “mild acid” Luria–Bertani medium. Differences in such resistances were also determined between the ∆phoP mutant and wild-type Salmonella strains to confirm the contribution of the PhoP/PhoQ system. The transcriptomic differences between the acid-adapted and ∆phoP strain were compared to explore the role of the PhoP/Q two-component system in regulating multi-stress resistance. Acid adaptation was found to increase the viability of Salmonella to lethal acid, heat and hyperosmotic treatments. In particular, acid adaptation significantly increased the resistance of Salmonella typhimurium to Polymyxin B, and such resistance can last for 21 days when the adapted strain was stored in meat extract medium at 4 °C. Transcriptomics analysis revealed 178 up-regulated and 274 down-regulated genes in the ∆phoP strain. The Salmonella infection, cationic antimicrobial peptide (CAMP) resistance, quorum sensing and two-component system pathways were down-regulated, while the bacterial tricarboxylic acid cycle pathways were up-regulated. Transcriptomics and RT-qPCR analyses revealed that the deletion of the phoP gene resulted in the down-regulation of the expression of genes related to lipid A modification and efflux pumps. These changes in the gene expression result in the change in net negative charge and the mobility of the cell membrane, resulting in enhanced CAMP resistance. The confirmation of multiple stress resistance under acid adaptation and the transcriptomic study in the current study may provide valuable information for the control of multiple stress resistance and meat safety. Full article