The development of fully biobased hydrogels obtained by simple routes and in the absence of toxic or environmentally harmful reagents is a major challenge in meeting new societal demands. In this work, we discuss the development of hydrogels made from cellulose nanocrystals (CNCs) and xyloglucan (XG), two non-toxic, renewable, and biobased components. We present three strategies to fine-tune the functional properties. The first one consists in varying the XG/CNC ratio that leads to the modulation of the mechanical properties of hydrogels as well as a better comprehension of the gel mechanism formation. The second relies on tuning the XG chains’ interaction by enzymatic modification to achieve thermoresponsive systems. Finally, the third one is based on the increase in the hydrogel solid content by osmotic concentration. The high-solid-content gels were found to have very high mechanical properties and self-healing properties that can be used for molding materials. Overall, these approaches are a case study of potential modifications and properties offered by biobased nanocolloidal hydrogels. Full article

Background: The association of periodontal disease and diabetes is a subject of intense research in terms of etiopathology and treatment options. This research aimed to evaluate the modulation of the local inflammatory status by two natural extracts, curcumin (Cu) and rutin (R), in an experimentally induced diabetes and periodontal disease in Wistar rats. Methods: Fifty Wistar albino rats were randomly assigned to five groups: Control (C), Diabetes-associated Periodontal Disease (DP), Diabetes-associated Periodontal Disease treated with Curcumin (DPCu), Diabetes-associated Periodontal Disease treated with Rutin (DPR), and Diabetes-associated Periodontal Disease treated with both Curcumin and Rutin (DPCuR). Gingival samples were collected from all rats, and immunohistochemical markers CD3, CD20, and CD34 were evaluated to assess the local inflammatory infiltrate. Descriptive statistics were applied (SPSS24 Software, Armonk, NY, USA). Results: Rutin, alone or combined with Curcumin, reduced CD3-positive cell levels. Curcumin demonstrated superior efficacy in reducing CD20-positive cells. The combination of Curcumin and Rutin had the most important impact on both markers. Curcumin notably increased immature CD34-positive cell levels. Conclusions: Curcumin and Rutin, either alone or together, hold potential for reducing local inflammation in diabetes-induced periodontal disease in Wistar rats. Full article

Urban planning is often influenced by industrial and construction activities, leading to a lack of attention to the planning and construction of urban parks, which results in prominent spatial layout problems. Urban parks, as an important part of the urban green space system, play a significant role in improving the ecological environment, promoting urban economic development, and enhancing the daily living standards of the people. As a typical representative of China’s second and third-tier cities, Nanchang’s analysis of the evolution process of urban landscape patterns has reference significance for other similar cities in China. This paper is based on the theoretical foundations of landscape ecology, human geography, and urban planning, and analyzes the evolution of the park landscape pattern in the central urban area of Nanchang from 1999 to 2019 from the perspective of urban context, revealing the driving mechanisms. It provides important references and bases for the further optimization and construction development of the park landscape pattern in Nanchang. The results show that the park area and number in the central urban area of Nanchang have significantly increased, with the overall layout evolving from “central aggregation” to “core aggregation in each area”, and from “central scarcity, more on the periphery” to “inward concentration, outward diffusion”. However, the distribution of various types of parks is uneven, and there is a lack of green corridor links between parks. The park landscape pattern is driven by multiple factors such as natural factors of urban context, socio-economic factors, urban construction factors, historical policies, and related planning, which can have positive or negative effects. Incorporating relevant urban factors into the park planning system analysis can promote the benign development of urban context and park landscape layout, thereby achieving the “parkification” of the city. Full article

Indoor triaxial tests on geogrid-reinforced clay elucidate the macroscopic changes in soil strength indices post-reinforcement, yet the underlying mechanisms of strength enhancement require further investigation. By conducting indoor triaxial tests and establishing a corresponding discrete element numerical model, we can delve into the fine-scale mechanisms of geogrid-reinforced soil. This includes analyzing changes in fine-scale parameters such as porosity, the coordination number, and contact stress between soil particles. The findings suggest that an increase in the number of geogrid reinforcement layers leads to a more pronounced improvement in peak strength and cohesion, albeit with minimal impact on the internal friction angle of the specimens. Furthermore, analysis of the triaxial test curves of reinforced soils indicates that the stress–strain relationship adheres to the Duncan–Chang model. Parameters derived from this model have been validated against experimental data, confirming their accuracy. The discrete element model was used to analyze the variations in fine-scale parameters such as porosity and coordination number. It revealed that reinforcement reduces the fluctuation amplitude of porosity and significantly increases the number of particle contacts, resulting in a denser soil structure. Further analysis of the change in contact stress between particles in the discrete element model revealed that the contact force between particles increased significantly after reinforcement and that the reinforcement played a role in restraining the soil particles and dispersing the reinforcement stress, which explains the increase in the strength of the mesh-reinforced clays from another perspective. This further elucidates the strength enhancement mechanism in geogrid-reinforced clay, offering a new perspective on the mechanical behavior and strength development of such materials. Full article

Glioblastoma multiforme (GBM) represents the deadliest tumor among brain cancers. It is a solid tumor characterized by uncontrolled cell proliferation generating the hypoxic niches in the cancer core. By inducing the transcription of hypoxic inducible factor (HIF), hypoxia triggers many signaling cascades responsible for cancer progression and aggressiveness, including enhanced expression of vascular endothelial growth factor (VEGF) or antioxidant enzymes, such as heme oxygenase-1 (HO-1). The present work aimed to investigate the link between HO-1 expression and the hypoxic microenvironment of GBM by culturing two human glioblastoma cell lines (U87MG and A172) in the presence of a hypoxic mimetic agent, deferoxamine (DFX). By targeting hypoxia-induced HO-1, we have tested the effect of a novel acetamide-based HO-1 inhibitor (VP18/58) on GBM progression. Results have demonstrated that hypoxic conditions induced upregulation and nuclear expression of HO-1 in a cell-dependent manner related to malignant phenotype. Moreover, our data demonstrated that the HO-1 inhibitor counteracted GBM progression by modulating the HIFα/HO-1/VEGF signaling cascade in cancer cells bearing more malignant phenotypes. Full article

This research study aimed to evaluate the legibility of Arabic road signage using an eye-tracking approach within a virtual reality (VR) environment. The study was conducted in a controlled setting involving 20 participants who watched two videos using the HP Omnicept Reverb G2. The VR device recorded eye gazing details in addition to other physiological data of the participants, providing an overlay of heart rate, eye movement, and cognitive load, which in combination were used to determine the participants’ focus during the experiment. The data were processed through a schematic design, and the final files were saved in .txt format, which was later used for data extraction and analysis. Through the execution of this study, it became apparent that employing eye-tracking technology within a VR setting offers a promising method for assessing the legibility of road signs. The outcomes of the current research enlightened the vital role of legibility in ensuring road safety and facilitating effective communication with drivers. Clear and easily comprehensible road signs were found to be pivotal in delivering timely information, aiding navigation, and ultimately mitigating accidents or confusion on the road. As a result, this study advocates for the utilization of VR as a valuable platform for enhancing the design and functionality of road signage systems, recognizing its potential to contribute significantly to the improvement of road safety and navigation for drivers. Full article

Green stormwater infrastructure (GSI) is a key approach to greening and cooling high-density blocks. Previous studies have focused on the impact of a single GSI on thermal comfort on sunny days, ignoring rainwater’s role and GSI combinations. Therefore, based on measured data of a real urban area in Nanjing, China, this study utilized 45 single-GSI and combination simulation scenarios, as well as three local climate zone (LCZ) baseline scenarios to compare and analyze three high-density blocks within the city. Among the 32 simulations specifically conducted in LCZ1 and LCZ2, 2 of them were dedicated to baseline scenario simulations, whereas the remaining 30 simulations were evenly distributed across LCZ1 and LCZ2, with 15 simulations allocated to each zone. The physiological equivalent temperature (PET) was calculated using the ENVI-met specification to evaluate outdoor thermal comfort. The objective of this research was to determine the optimal GSI combinations for different LCZs, their impact on pedestrian thermal comfort, GSI response to rainwater, and the effect of GSI on pedestrian recreation areas. Results showed that GSI combinations are crucial for improving thermal comfort in compact high-rise and mid-rise areas, while a single GSI suffices in low-rise areas. In extreme heat, rainfall is vital for GSI’s effectiveness, and complex GSI can extend the thermal comfort improvement time following rainfall by more than 1 h. Adding shading and trees to GSI combinations maximizes thermal comfort in potential crowd activity areas, achieving up to 54.23% improvement. Future GSI construction in high-density blocks should focus on different combinations of GSI based on different LCZs, offering insights for GSI planning in Southeast Asia. Full article

(1) Background: currently, the advantages of bulk-fill resin composite and high-viscosity glass ionomer materials have increased their use in dentistry; accordingly, their mechanical, surface, and optical properties have become more important. This study aimed to evaluate the mechanical, surface, and optical properties of three different restorative materials (a high-viscosity bulk-fill resin composite (TNC), a flowable bulk-fill resin composite (EBF), and a high-viscosity glass ionomer (FIX)) after application using different techniques (control, heat application, and ultrasonic activation). (2) Methods: specimens were prepared to assess the color stability and surface roughness (n = 12). The specimens were immersed in two different solutions for 14 days. For the compressive strength test, specimens were prepared using a Teflon mold (n = 12). (3) Results: among the specimens applied according to the manufacturer’s instructions and immersed in distilled water, it was observed that the surface roughness values of FIX on the 7th day were statistically significantly higher than the other materials (p < 0.05). The compressive strength values of EBF applied using ultrasonic activation were significantly lower than those of EBF applied using the other techniques (p < 0.05). (4) Conclusions: coffee can negatively affect the color stability of restorative materials, but discoloration may vary depending on differences in the content of the material. All materials evaluated in this study exhibited clinically acceptable surface roughness values. It can be concluded that flowable bulk-fill resin composite is the most durable material in terms of compressive strength, so it can be used in the restoration of posterior teeth, especially those exposed to intensive stress. Full article

The enticing aroma of truffles is a key factor for their culinary value. Although all truffle species tend to be pricy, the most intensely aromatic species are the most sought after. Research into the aroma of truffles encompasses various disciplines including chemistry, biology, and sensory science. This study focusses on the chemical composition of the aroma of black truffles (Tuber melanosporum) and the changes occurring under different storage conditions. For this, truffle samples were stored under different treatments, at different temperatures, and measured over a total storage time of 12 days. Measurements of the truffle aroma profiles were taken with SPME/GC–MS at regular intervals. To handle the ample data collected, a systematic approach utilizing multivariate data analysis techniques was taken. This approach led to a vast amount of data which we made publicly available for future exploration. Results reveal the complexity of aroma changes, with 695 compounds identified, highlighting the need for a comprehensive understanding. Principal component analyses offer initial insights into truffle composition, while individual compounds may serve as markers for age (formic acid, 1-methylpropyl ester), freshness (2-Methyl-1-propanal; 1-(methylthio)-propane), freezing (tetrahydrofuran), salt treatment (1-chloropentane), or heat exposure (4-hydroxy-3-methyl-2-butanone). This research suggests that heat treatment or salt contact significantly affects truffle aroma, while freezing and cutting have less pronounced effects in comparison. The enrichment of compounds showing significant changes during storage was investigated with a metabolomic pathway analysis. The involvement of some of the enriched compounds on the pyruvate/glycolysis and sulfur pathways was shown. Full article

Waste treatment and valorization have become crucial for sustainable development towards a circular economy. As an alternative, biochar production is a promising process to convert wastes into a valuable product that presents several potential applications to cope with environmental problems. Biochar in recent years has been the subject of many studies, which have leveraged the number of patents and the industrial interest in this process. Against this background, this overview aimed: (i) to identify the advances in biochar research; (ii) to assess the number of patents on biochar over the years; (iii) to look at the industrial production of biochar worldwide; (iv) to detect the potential for biochar production in Brazil regarding waste biomass availability; and (v) to discuss the potential of biochar in contributing to reach some Sustainable Development Goals (SDGs). The holistic analysis presented here suggests that progress has been made in research, patent development, and industrial implementation of biochar, and that its potential role in achieving certain SDGs is noteworthy. Therefore, this overview can be useful in guiding future research about biochar to improve the knowledge of the different branches in this field. Full article

Some researchers in past years have tried to develop a simplified method for analyzing soil liquefaction. However, the correctness of the pore water pressure in the model will affect the results. In addition, the formulas derived are not easy, and the exact parameters of the model are difficult to obtain. This study used a mass-spring-damping system to simulate the repeated strain of liquefaction cyclic triaxial tests. Because the model is simple and the parameters are easy to understand and obtain, it also shows the extensibility of this model. During the parameter study, damping coefficient c and spring coefficient k parameters decreased with the increasing cyclic number. Preliminary results of the research show that this model can further simulate the repeated strain obtained by cyclic triaxial tests without considering the variation of effective stress during cyclic loading. Four samples were used to verify the model’s correctness, and their boring sites were found in Yunlin areas, Taiwan. Simulation results show that the spring-damping system is feasible for simulated cyclic triaxial tests because the simulated results correlate to the testing results in trend. Generally, the first cycle number simulation will be less accurate because the pore water pressure of the specimen changes rapidly when the performance has just started. In contrast, the increase in subsequent cycles may be biased due to cyclic stress variation and soil plasticity during simulation. In the future, pure sand specimens created in the laboratory will be suggested for simulation. Full article

Both arbuscular mycorrhizal (AM) fungi and root hairs are crucial in facilitating plant uptake of phosphorus (P), while it is unclear whether and how they respond to varying P supplies. In order to explore how AM fungal colonization and root hair development are affected by substrate P supply, trifoliate orange (Poncirus trifoliata) seedlings were inoculated with AM fungus Rhizophagus intraradices and grown under low, moderate, and high P conditions; then, root hair morphological features and AM fungal colonization were measured. Following 120 days of AM fungal inoculation, root hair density, root hair length, AM fungal colonization rate, arbuscule colonization rate, and AM fungal colonization frequency all increased significantly under P-deficient conditions but decreased under high P conditions. Moreover, the colonization of AM fungi had a major impact on root hair formation by altering the expression of related genes and the growth of epidermal cells. The effect of AM fungi was dependent on P supply levels, as evidenced by the fact that root hair density and length increased at high P levels but decreased at low P levels. As a result, root hairs may serve as a preferential site for AM fungal colonization, and their morphology could influence the early stage of AM symbiosis establishment. Full article

Biomarkers for evaluating tumor response to therapy and estimating the risk of disease relapse represent tremendous areas of clinical need. To evaluate treatment efficacy, tumor response is routinely assessed using different imaging modalities like positron emission tomography/computed tomography or magnetic resonance imaging. More recently, the development of circulating tumor DNA detection assays has provided a minimally invasive approach to evaluate tumor response and prognosis through a blood test (liquid biopsy). Integrating imaging- and circulating tumor DNA-based biomarkers may lead to improvements in the prediction of patient outcomes. For this mini-review, we searched the scientific literature to find original articles that combined quantitative imaging and circulating tumor DNA biomarkers to build prediction models. Seven studies reported building prognostic models to predict distant recurrence-free, progression-free, or overall survival. Three discussed building models to predict treatment response using tumor volume, pathologic complete response, or objective response as endpoints. The limited number of articles and the modest cohort sizes reported in these studies attest to the infancy of this field of study. Nonetheless, these studies demonstrate the feasibility of developing multivariable response-predictive and prognostic models using regression and machine learning approaches. Larger studies are warranted to facilitate the building of highly accurate response-predictive and prognostic models that are generalizable to other datasets and clinical settings. Full article

Background: Leadership in any managerial position that a nurse may hold appears to be closely connected to fostering positive and productive work environments within healthcare settings. However, not all nurse managers are characterized by leadership, and not all nurse leaders are nurse managers. In countries, such as Mexico, those who occupy these roles have barely sufficient training in management, are mainly characterized by their experience in one clinical specialty and their vocation for leadership is not a requirement. Our study aims to understand how the leadership vocation that some nurse managers have in their daily practice could impact the work environments of healthcare organizations in Mexico. Methods: A qualitative design was carried out through a grounded theory approach based on Corbin & Strauss, interviewing individually 13 nurse managers with representative experience in Mexican public sector hospitals. Results: According to the interviewees, the meaning of vocation is based mainly on the love for their profession, on the vocation of being a nurse and caring for people, as well as on the development of their own natural leadership potential for decision-making in practice. This situation produces positive feedback on themselves by generating productive effects in the work environment, consequently strengthening them to better organize professional resources and therefore producing improvements. Conclusions: The nurse manager’s vocation of leadership derives from both the vocation of being a practicing nurse and the vocation for being a leader, positively impacting and productively contributing to the improvement of the work environment. Consequently, nurse managers with vocation for leadership should be the gold standard role in any healthcare organization. This study was retrospectively registered with the (registros-OSF-bmyvz-v1) on the (26 July 2023) registration number (10.17605/OSF.IO/BMYVZ). Full article

Axial postural abnormalities (APAs), characterized by their frequency, disabling nature, and resistance to pharmacological treatments, significantly impact Parkinson’s disease and atypical Parkinsonism patients. Despite advancements in diagnosing, assessing, and understanding their pathophysiology, managing these complications remains a significant challenge. Often underestimated by healthcare professionals, these disturbances can exacerbate disability. This systematic review assesses botulinum toxin treatments’ effectiveness, alone and with rehabilitation, in addressing APAs in Parkinson’s disease, utilizing MEDLINE (PubMed), Web of Science, and SCOPUS databases for source material. Of the 1087 records retrieved, 16 met the selection criteria. Most research has focused on botulinum toxin (BoNT) as the primary treatment for camptocormia and Pisa syndrome, utilizing mostly observational methods. Despite dose and injection site variations, a common strategy was using electromyography-guided injections, occasionally enhanced with ultrasound. Patients with Pisa syndrome notably saw consistent improvements in APAs and pain. However, studies on the combined effects of botulinum toxin and rehabilitation are limited, and antecollis is significantly under-researched. These findings recommend precise BoNT injections into hyperactive muscles in well-selected patients by skilled clinicians, avoiding compensatory muscles, and underscore the necessity of early rehabilitation. Rehabilitation is crucial in a multidisciplinary approach to managing APAs, highlighting the importance of a multidisciplinary team of experts. Full article

With the urgent global need to limit warming to 2 °C as well as a localized need in our case study to address rising energy demand amid electrical and thermal network limitations, a critical examination of demand-side energy reductions and the concept of energy sufficiency is needed. This paper contributes to the sparse literature on bottom-up analysis by utilizing Iceland—a leader in renewable energy generation—as a case study to explore the socio-economic factors influencing energy footprints. Our findings reveal significant energy footprints across various consumption domains, particularly housing and mobility, influenced by income levels, urbanization, and lifestyle choices. The study highlights the paradox of a high renewable energy supply leading to potential misconceptions regarding abundant and low-cost energy, resulting in substantial energy consumption-related environmental impacts. Using detailed household consumption survey data, this research provides insights crucial for developing sustainable energy policies that not only target technological advancements but also address the need for a reduction in energy demand and a shift towards energy sufficiency. This work marks a contribution to the literature through the provision of a case study of low income inequality and high energy footprints in a highly renewable energy system context. Further, this work is useful for Icelandic and international policymakers to understand in such high-demand contexts which consumption domains would be most relevant for sufficiency policies. This comprehensive analysis opens pathways for future research to further explore the intersections of energy consumption, socio-economic factors, and well-being, offering a nuanced understanding necessary for crafting sufficiency and demand-side policies aimed at a sustainable energy future. Full article

Malaria remains an important and challenging infectious disease, and novel antimalarials are required. Benzyl isothiocyanate (BITC), the main breakdown product of benzyl glucosinolate, is present in all parts of Tropaeolum majus L. (T. majus) and has antibacterial and antiparasitic activities. To our knowledge, there is no information on the effects of BITC against malaria. The present study evaluates the antimalarial activity of aqueous extracts of BITC and T. majus seeds, leaves, and stems. We used flow cytometry to calculate the growth inhibition (GI) percentage of the extracts and BITC against unsynchronized cultures of the chloroquine-susceptible Plasmodium falciparum 3D7 − GFP strain. Extracts and/or compounds with at least 70% GI were validated by IC50 estimation against P. falciparum 3D7 − GFP and Dd2 (chloroquine-resistant strain) unsynchronized cultures by flow cytometry, and the resistance index (RI) was determined. T. majus aqueous extracts showed some antimalarial activity that was higher in seeds than in leaves or stems. BITC’s GI was comparable to chloroquine’s. BITC’s IC50 was similar in both strains; thus, a cross-resistance absence with aminoquinolines was found (RI < 1). BITC presented features that could open new avenues for malaria drug discovery. Full article

The miniaturization and low power consumption characteristics of RF MEMS (Radio Frequency Microelectromechanical System) switches provide new possibilities for the development of microsatellites and nanosatellites, which will play an increasingly important role in future space missions. This paper provides a comprehensive review of RF MEMS switches in satellite communication, detailing their working mechanisms, performance optimization strategies, and applications in reconfigurable antennas. It explores various driving mechanisms (electrostatic, piezoelectric, electromagnetic, thermoelectric) and contact mechanisms (capacitive, ohmic), highlighting their advantages, challenges, and advancements. The paper emphasizes strategies to enhance switch reliability and RF performance, including minimizing the impact of shocks, reducing driving voltage, improving contacts, and appropriate packaging. Finally, it discusses the enormous potential of RF MEMS switches in future satellite communications, addressing their technical advantages, challenges, and the necessity for further research to optimize design and manufacturing for broader applications and increased efficiency in space missions. The research findings of this review can serve as a reference for further design and improvement of RF MEMS switches, which are expected to play a more important role in future aerospace communication systems. Full article

The German health authorities’ guidelines for medical devices in 2012 highlighted the importance of cleaning ultrasound probes, emphasizing their validation and reliability. In addition to automated and validated options, alternative manual methods such as wipe disinfection have gained traction due to their independence from additional hardware. The study examines the effectiveness of a manual cleaning process using wipes, addressing concerns raised by the Robert Koch Institute regarding the lack of validation for wipe disinfection of semi-critical devices. The EQUINOS colored wipe disinfection kit identified wetting gaps in all cleanings across four probes tested. The results indicate significant challenges in ensuring complete surface wetting, particularly in complex device parts such as clip-on areas and fixtures for additional biopsy attachments, suggesting that manual methods alone may not adequately mitigate the risk of infection transmission (p value < 0.0001). The study concludes that while manual disinfection methods are a commonly used alternative to automated reprocessing, there is a critical need for enhanced training and potentially the development of more effective manual disinfection techniques or colored wipes to ensure patient safety and compliance with healthcare hygiene standards. Full article

Compared to the number of studies on the neoplasms of laboratory rodents, fewer studies have focused on spontaneous neoplasms in pet rodents. Notably, the mouse mammary tumor virus (MMTV) is associated with mammary tumors in rodents. In this study, 77 tumors and tumor-like lesions of biopsy samples were collected from 70 pet rodents, including hamsters (n = 47), guinea pigs (n = 16), unknown species (n = 4), rats (n = 2), and a gerbil. Fifty tumors were collected from 47 hamsters, in which the most common tumors were mammary tumors (13/50), followed by fibrosarcoma (9/50), mast cell tumors (4/50), and squamous cell carcinoma (4/50). The collected subtypes of mammary tumors in hamsters included tubular carcinoma (n = 5), tubular adenoma (n = 4), carcinoma and malignant myoepithelioma (n = 1), simple tubular carcinoma (n = 1), adenosquamous carcinoma (n = 1), and tubulopapillary adenoma (n = 1). In addition, twenty tumors were collected from guinea pigs, in which the most common tumor was lipoma (6/20), followed by adenocarcinoma of the mammary gland (4/20), trichofolliculoma (2/20), and collagenous hamartomas (2/20). In guinea pigs, the subtypes of mammary gland tumors were tubular carcinoma (n = 2), tubular and solid carcinoma (n = 1), and tubulopapillary carcinoma (n = 1). In 20 cases of mammary tumors, MMTV was not detected, implicating no evidence of MMTV infection in mammary oncogenesis in pet rodents in Taiwan. Full article

Monitoring vital rates allows managers to estimate trends in growth rates of ungulate populations. However, connecting the influence of nutrition on ungulate demography is challenging. Noninvasive sampling offers a low-cost, low-effort alternative for measuring nutritional indices, allowing for an increased understanding of the mechanistic relationships between environmental factors, nutrition, and specific population vital rates. We examined the temporal influence of intrinsic and extrinsic factors on pronghorn (Antilocapra americana) fawn recruitment. We collected fresh fecal samples from adult female pronghorn in five subpopulations spanning three sampling periods associated with critical maternal life-history stages (late gestation, early lactation, breeding season) for 2 years to investigate both intra- and interannual influences. Intrinsic factors were fecal glucocorticoid metabolites (FGMs), nutritional indices (fecal nitrogen (FN) and 2,6-diaminopimelic acid (DAPA)), and dietary composition (protein intake of forbs, graminoids, legumes, other, shrubs), while the extrinsic factor was vegetative greenness (normalized difference vegetation index (NDVI)). We found variations in DAPA, protein intake of forbs, variation in forb protein intake, and protein intake of legumes during late gestation positively influenced fawn recruitment. Fecal nitrogen during early lactation showed the strongest positive influence on the recruitment of any measured parameter. Finally, breeding season NDVI and the variation in DAPA values positively influenced the subsequent year’s fawn recruitment. Our longitudinal study enabled us to investigate which parameter was most important to specific periods of fawn development and recruitment. We combined the results across five subpopulations, but interpretation and subsequent management decisions should be made at the subpopulation level such that pronghorn subpopulations with low recruitment can be positively influenced by increasing nitrogen on the landscape available to adult females during the early lactation period. As the use of noninvasive monitoring methods continues to expand, we believe our methodologies and results can be broadly applied to other ungulate monitoring programs. Full article