The 2023 MDPI Annual Report has
been released!
 
18 pages, 2121 KiB  
Article
Molecular Dynamics Simulation on Solidification Microstructure and Tensile Properties of Cu/SiC Composites
by Wanjun Yan, Yuhang Lu, Tinghong Gao, Junjie Wang, Xin Tang and Nan Wang
Molecules 2024, 29(10), 2230; https://doi.org/10.3390/molecules29102230 (registering DOI) - 9 May 2024
Abstract
The shape of ceramic particles is one of the factors affecting the properties of metal matrix composites. Exploring the mechanism of ceramic particles affecting the cooling mechanical behavior and microstructure of composites provides a simulation basis for the design of high-performance composites. In [...] Read more.
The shape of ceramic particles is one of the factors affecting the properties of metal matrix composites. Exploring the mechanism of ceramic particles affecting the cooling mechanical behavior and microstructure of composites provides a simulation basis for the design of high-performance composites. In this study, molecular dynamics methods are used for investigating the microstructure evolution mechanism in Cu/SiC composites containing SiC particles of different shapes during the rapid solidification process and evaluating the mechanical properties after cooling. The results show that the spherical SiC composites demonstrate the highest degree of local ordering after cooling. The more ordered the formation is of face-centered-cubic and hexagonal-close-packed structures, the better the crystallization is of the final composite and the less the number of stacking faults. Finally, the results of uniaxial tensile in three different directions after solidification showed that the composite containing spherical SiC particles demonstrated the best mechanical properties. The findings of this study provide a reference for understanding the preparation of Cu/SiC composites with different shapes of SiC particles as well as their microstructure and mechanical properties and provide a new idea for the experimental and theoretical research of Cu/SiC metal matrix composites. Full article
(This article belongs to the Topic Advances in Computational Materials Sciences)
14 pages, 524 KiB  
Review
Some Recent Advances in Germanium Recovery from Various Resources
by Francisco Jose Alguacil and Jose Ignacio Robla
Metals 2024, 14(5), 559; https://doi.org/10.3390/met14050559 (registering DOI) - 9 May 2024
Abstract
Though nowadays germanium does not reach the range of popularity of other metals, i.e., rare earth elements, its utility in target industries makes it a strategic metal. Though germanium can be found in a series of raw materials, the principal source for its [...] Read more.
Though nowadays germanium does not reach the range of popularity of other metals, i.e., rare earth elements, its utility in target industries makes it a strategic metal. Though germanium can be found in a series of raw materials, the principal source for its recovery is from secondary wastes of the zinc industry; also, the recyclability of germanium-bearing waste materials is becoming of interest. In this recovery and due to the size of the target materials, because the diffusion and reaction are to be considered, hydrometallurgy performs a key role in achieving this goal. The present work reviews the most recent applications (2023 and 2024 years) of hydrometallurgical operations on the recovery of germanium from different solid and liquid sources. Full article
(This article belongs to the Special Issue Advances in Sustainable Hydrometallurgy)
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23 pages, 6506 KiB  
Article
Selection of the Discriming Feature Using the BEMD’s BIMF for Classification of Breast Cancer Mammography Image
by Fatima Ghazi, Aziza Benkuider, Fouad Ayoub and Khalil Ibrahimi
BioMedInformatics 2024, 4(2), 1202-1224; https://doi.org/10.3390/biomedinformatics4020066 (registering DOI) - 9 May 2024
Abstract
Mammogram exam images are useful in identifying diseases, such as breast cancer, which is one of the deadliest cancers, affecting adult women around the world. Computational image analysis and machine learning techniques can help experts identify abnormalities in these images. In this work [...] Read more.
Mammogram exam images are useful in identifying diseases, such as breast cancer, which is one of the deadliest cancers, affecting adult women around the world. Computational image analysis and machine learning techniques can help experts identify abnormalities in these images. In this work we present a new system to help diagnose and analyze breast mammogram images. To do this, the system a method the Selection of the Most Discriminant Attributes of the images preprocessed by BEMD “SMDA-BEMD”, this entails picking the most pertinent traits from the collection of variables that characterize the state under study. A reduction of attribute based on a transformation of the data also called an extraction of characteristics by extracting the Haralick attributes from the Co-occurrence Matrices Methods “GLCM” this reduction which consists of replacing the initial set of data by a new reduced set, constructed at from the initial set of features extracted by images decomposed using Bidimensional Empirical Multimodal Decomposition “BEMD”, for discrimination of breast mammogram images (healthy and pathology) using BEMD. This decomposition makes it possible to decompose an image into several Bidimensional Intrinsic Mode Functions “BIMFs” modes and a residue. The results obtained show that mammographic images can be represented in a relatively short space by selecting the most discriminating features based on a supervised method where they can be differentiated with high reliability between healthy mammographic images and pathologies, However, certain aspects and findings demonstrate how successful the suggested strategy is to detect the tumor. A BEMD technique is used as preprocessing on mammographic images. This suggested methodology makes it possible to obtain consistent results and establishes the discrimination threshold for mammography images (healthy and pathological), the classification rate is improved (98.6%) compared to existing cutting-edge techniques in the field. This approach is tested and validated on mammographic medical images from the Kenitra-Morocco reproductive health reference center (CRSRKM) which contains breast mammographic images of normal and pathological cases. Full article
(This article belongs to the Special Issue Feature Papers on Methods in Biomedical Informatics)
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13 pages, 753 KiB  
Article
Advancing Age May Decrease Mitochondrial Activity in Cumulus Cells
by Suwichaya Jitngamsujarit, Lingling Salang, Charupong Saengboonmee, Supannika Sorin, Kanyarat Thithuan, Thanida Pongsritasana and Sineenart Sukkasame
J. Clin. Med. 2024, 13(10), 2800; https://doi.org/10.3390/jcm13102800 (registering DOI) - 9 May 2024
Abstract
Background: The goal of this study was to compare mitochondrial activity in cumulus cells (CCs) between young and advancing-aged women, the factors that affect mitochondrial activity, and their association with blastocyst quality. Materials and methods: This prospective study included 80 infertile women who [...] Read more.
Background: The goal of this study was to compare mitochondrial activity in cumulus cells (CCs) between young and advancing-aged women, the factors that affect mitochondrial activity, and their association with blastocyst quality. Materials and methods: This prospective study included 80 infertile women who underwent ICSI between May and October 2023. Participants were divided into two groups: older and younger than 38. The oocyte mitochondrial activity from CCs was evaluated using MitoTracker, and the mean fluorescence intensity (MFI) was also evaluated. Results: The univariate and multivariate analyses revealed a significant difference in the MFI between the woman ≥ 38 age group and the lower age group (162.68 ± 79.87 vs. 228.39± 121.38; p-value = 0.005; 95%CI 19.97, 111.45). The factors that affected the MFI were women ≥ 38 years of age (p-value = 0.005; 95%CI −111.45, −19.91), total gonadotropin dosages (p-value = 0.006; 95%CI −0.08, 0.01), and gonadotropin-releasing hormone agonist (GnRHa) triggering (p-value = 0.006; 95%CI 36.46, 210.06). However, only women aged ≥38 years remained statistically significant after a multivariable regression analysis (p-value = 0.014; 95%CI −121.00, −14.30). In addition, only male age (mean age ± SD = 38.26 ± 5.13) was associated with high blastocyst quality in univariate and mixed multivariate analyses (OR 0.91; 95%CI 0.56, 3.04). The chemical pregnancy rate was not significantly different between the two age groups (34.5% vs. 56.7%; p-value = 0.162; 95%CI 0.2, 1.30). Conclusion: Advancing age decreased mitochondrial activity in CCs but did not affect blastocyst quality. By contrast, male age may be a predictor of high-grade blastocyst quality. Full article
23 pages, 3853 KiB  
Review
The Use of Spectroscopic Methods to Study Organic Matter in Virgin and Arable Soils: A Scoping Review
by Evgeny Lodygin and Evgeny Abakumov
Agronomy 2024, 14(5), 1003; https://doi.org/10.3390/agronomy14051003 (registering DOI) - 9 May 2024
Abstract
The use of modern spectroscopic methods of analysis, which provide extensive information on the chemical nature of substances, significantly expands our understanding of the molecular composition and properties of soil organic matter (SOM) and its transformation and stabilization processes in various ecosystems and [...] Read more.
The use of modern spectroscopic methods of analysis, which provide extensive information on the chemical nature of substances, significantly expands our understanding of the molecular composition and properties of soil organic matter (SOM) and its transformation and stabilization processes in various ecosystems and geochemical conditions. The aim of this review is to identify and analyze studies related to the application of nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopy techniques to study the molecular composition and transformation of organic matter in virgin and arable soils. This article is mainly based on three research questions: (1) Which NMR spectroscopy techniques are used to study SOM, and what are their disadvantages and advantages? (2) How is the NMR spectroscopy technique used to study the molecular structure of different pools of SOM? (3) How is ESR spectroscopy used in SOM chemistry, and what are its advantages and limitations? Relevant studies published between 1996 and 2024 were searched in four databases: eLIBRARY, MDPI, ScienceDirect and Springer. We excluded non-English-language articles, review articles, non-peer-reviewed articles and other non-article publications, as well as publications that were not available according to the search protocols. Exclusion criteria for articles were studies that used NMR and EPR techniques to study non-SOM and where these techniques were not the primary methods. Our scoping review found that both solid-state and solution-state NMR spectroscopy are commonly used to study the structure of soil organic matter (SOM). Solution-phase NMR is particularly useful for studying soluble SOM components of a low molecular weight, whereas solid-phase NMR offers advantages such as higher 13C atom concentration for stronger signals and faster analysis time. However, solution-phase NMR has limitations including sample insolubility, potential signal aggregation and reduced sensitivity and resolution. Solid-state NMR is better at detecting non-protonated carbon atoms and identifying heterogeneous regions within structures. EPR spectroscopy, on the other hand, offers significant advantages in experimental biochemistry due to its high sensitivity and ability to provide detailed information about substances containing free radicals (FRs), aiding in the assessment of their reactivity and transformations. Understanding the FR structure in biopolymers can help to study the formation and transformation of SOM. The integration of two- and three-dimensional NMR spectroscopy with other analytical methods, such as chromatography, mass spectrometry, etc., provides a more comprehensive approach to deciphering the complex composition of SOM than one-dimensional techniques alone. Full article
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12 pages, 556 KiB  
Article
Prevalences and Interrelationships of Post COVID-19 Fatigue, Sleep Disturbances, and Depression in Healthy Young and Middle-Aged Adults
by Changhwan Kim, Jae Young Moon, Sung Hyun Kim, Sun-Hyung Kim, Youjin Chang, Woo Hyun Cho, Won-Young Kim, Sun Jung Kwon, Ho Cheol Kim, Kwang Ha Yoo and Young Seok Lee
J. Clin. Med. 2024, 13(10), 2801; https://doi.org/10.3390/jcm13102801 (registering DOI) - 9 May 2024
Abstract
An evaluation of the persistence of symptoms following COVID-19 in economically active young and middle-aged adults is crucial due to its significant socioeconomic impact resulting from compromised work performance. Methods: A prospective, multicenter study at 12 South Korean hospitals from January to December [...] Read more.
An evaluation of the persistence of symptoms following COVID-19 in economically active young and middle-aged adults is crucial due to its significant socioeconomic impact resulting from compromised work performance. Methods: A prospective, multicenter study at 12 South Korean hospitals from January to December 2022 involved telephone interviews along with validated questionnaires. Results: Among 696 participants with a median age of 32 and no prior diagnoses, 30% of participants experienced persistent fatigue, while 21.4% suffered from sleep disturbance at 6 months following infection. Additionally, approximately 25% of the participants exhibited depression that endured for up to 6 months. Symptomatic individuals at 3 months exhibited a significantly higher prevalence of persistent fatigue, sleep disturbances, and depression at 6 months compared to those who remained asymptomatic. Notably, sleep disturbance and persistent fatigue at 3 months emerged as significant independent predictors of the presence of depression at 6 months. Conclusions: Even among young and middle-aged healthy adults, prolonged fatigue, sleep disturbance, and depression exhibit a significant prevalence and persisted for up to 6 months. Therefore, implementing a workplace management protocol for these symptoms is essential to mitigate the socioeconomic burden caused by the impairment of work efficiency. Full article
(This article belongs to the Section Pulmonology)
14 pages, 486 KiB  
Article
Palladium-Catalyzed Arylations towards 3,6-Diaryl-1,3a,6a-triazapentalenes and Evaluation of Their Fluorescence Properties
by Yingchun Wang, Tomas Opsomer, Flip de Jong, Davy Verhaeghe, Maarten Mulier, Luc Van Meervelt, Mark Van der Auweraer and Wim Dehaen
Molecules 2024, 29(10), 2229; https://doi.org/10.3390/molecules29102229 (registering DOI) - 9 May 2024
Abstract
Methyl 4-(1,3a,6a-triazapentalen-3-yl)benzoate (TAP1) shows interesting properties as a small molecule fluorophore. In the search for post-functionalization methods, palladium-catalyzed arylation reactions were demonstrated. Direct CH arylation reactions of TAP1 with various aryl halides resulted in 3,6-diaryltriazapentalenes TAP4, although mostly in poor [...] Read more.
Methyl 4-(1,3a,6a-triazapentalen-3-yl)benzoate (TAP1) shows interesting properties as a small molecule fluorophore. In the search for post-functionalization methods, palladium-catalyzed arylation reactions were demonstrated. Direct CH arylation reactions of TAP1 with various aryl halides resulted in 3,6-diaryltriazapentalenes TAP4, although mostly in poor yields. Bromination of TAP1 followed by Suzuki coupling, on the other hand, requires a more delicate procedure, but gave arylated products with the same regiochemistry (TAP4) in moderate to good yields. The structure of 6-phenyltriazapentalene TAP4a was confirmed by crystallographic analysis. In addition, the effect of the C6 arylation on the fluorescent properties of 3-aryl-1,3a,6a-triazapentalenes was studied in dichloromethane at room temperature and in 2-methyltetrahydrofuran at 77K, while the photophysical properties of two saponified derivatives were measured in acetonitrile. Full article
(This article belongs to the Special Issue Heterocyclic Chemistry in Europe)
14 pages, 3206 KiB  
Article
Research on the Functional Microbe Activation System in a Post-Polymer Flooded Reservoir
by Yinsong Liu, Min Wang, Haiwen Wei, Xiaolin Wu, Zhaowei Hou, Xiumei Zhang and Erlong Yang
Processes 2024, 12(5), 967; https://doi.org/10.3390/pr12050967 (registering DOI) - 9 May 2024
Abstract
Further exploitation of the residual oil underground in post-polymer flooded reservoirs is attractive and challenging. Microbial-enhanced oil recovery (MEOR) is a promising strategy to enhance the recovery of residual oil in post-polymer flooded reservoirs. Identifying and selectively activating indigenous microorganisms with oil displacement [...] Read more.
Further exploitation of the residual oil underground in post-polymer flooded reservoirs is attractive and challenging. Microbial-enhanced oil recovery (MEOR) is a promising strategy to enhance the recovery of residual oil in post-polymer flooded reservoirs. Identifying and selectively activating indigenous microorganisms with oil displacement capabilities is an urgent requirement in the current design of efficient microbial-enhanced oil recovery technologies. This study combines high-throughput sequencing with functional network analysis to identify the core functional microbes within the reservoirs. Concurrently, it devises targeted activation strategies tailored to oligotrophic conditions through an analysis of environmental factor influences. The feasibility of these strategies is then validated through physical simulation experiments. With nutrient stimulation, the overall diversity of microorganisms decreases while the abundance of functional microorganisms increases. The core displacement results showed that the oil recovery factor increased by 3.82% on the basis of polymer flooding. In summary, this research has established a system for the efficient activation of functional microorganisms under oligotrophic conditions by utilizing bioinformatics, network analysis, and indoor simulation systems. This achievement will undoubtedly lay a solid foundation for the practical implementation of microbial enhancement techniques in the field. Full article
(This article belongs to the Section Energy Systems)
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20 pages, 8206 KiB  
Article
Long-Term Maintenance of Viable Human Endometrial Epithelial Cells to Analyze Estrogen and Progestin Effects
by Muhammad Assad Riaz, Franziska Louisa Kary, Alexandra Jensen, Felix Zeppernick, Ivo Meinhold-Heerlein and Lutz Konrad
Cells 2024, 13(10), 811; https://doi.org/10.3390/cells13100811 (registering DOI) - 9 May 2024
Abstract
There are fewer investigations conducted on human primary endometrial epithelial cells (HPEECs) compared to human primary endometrial stromal cells (HPESCs). One of the main reasons is the scarcity of protocols enabling prolonged epithelial cell culture. Even though it is possible to culture HPEECs [...] Read more.
There are fewer investigations conducted on human primary endometrial epithelial cells (HPEECs) compared to human primary endometrial stromal cells (HPESCs). One of the main reasons is the scarcity of protocols enabling prolonged epithelial cell culture. Even though it is possible to culture HPEECs in 3D over a longer period of time, it is technically demanding. In this study, we successfully established a highly pure, stable, and long-term viable human conditionally reprogrammed endometrial epithelial cell line, designated as eCRC560. These cells stained positive for epithelial markers, estrogen and progesterone receptors, and epithelial cell–cell contacts but negative for stromal and endothelial cell markers. Estradiol (ES) reduced the abundance of ZO-1 in a time- and dose-dependent manner, in contrast to the dose-dependent increase with the progestin dienogest (DNG) when co-cultured with HPESCs. Moreover, ES significantly increased cell viability, cell migration, and invasion of the eCRC560 cells; all these effects were inhibited by pretreatment with DNG. DNG withdrawal led to a significantly disrupted monolayer of eCRC560 cells in co-culture with HPESCs, yet it markedly increased the adhesion of eCRC560 to the human mesothelial MeT-5A cells. The long-term viable eCRC560 cells are suitable for in vitro analysis of HPEECs to study the epithelial compartment of the human endometrium and endometrial pathologies. Full article
(This article belongs to the Special Issue Molecular Advances and New Therapeutic Approaches in Endometriosis)
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14 pages, 321 KiB  
Article
Does Quantum Mechanics Require “Conspiracy”?
by Ovidiu Cristinel Stoica
Entropy 2024, 26(5), 411; https://doi.org/10.3390/e26050411 (registering DOI) - 9 May 2024
Abstract
Quantum states containing records of incompatible outcomes of quantum measurements are valid states in the tensor-product Hilbert space. Since they contain false records, they conflict with the Born rule and with our observations. I show that excluding them requires a fine-tuning to an [...] Read more.
Quantum states containing records of incompatible outcomes of quantum measurements are valid states in the tensor-product Hilbert space. Since they contain false records, they conflict with the Born rule and with our observations. I show that excluding them requires a fine-tuning to an extremely restricted subspace of the Hilbert space that seems “conspiratorial”, in the sense that (1) it seems to depend on future events that involve records (including measurement settings) and on the dynamical law (normally thought to be independent of the initial conditions), and (2) it violates Statistical Independence, even when it is valid in the context of Bell’s theorem. To solve the puzzle, I build a model in which, by changing the dynamical law, the same initial conditions can lead to different histories in which the validity of records is relative to the new dynamical law. This relative validity of the records may restore causality, but the initial conditions still must depend, at least partially, on the dynamical law. While violations of Statistical Independence are often seen as non-scientific, they turn out to be needed to ensure the validity of records and our own memories and, by this, of science itself. A Past Hypothesis is needed to ensure the existence of records and turns out to require violations of Statistical Independence. It is not excluded that its explanation, still unknown, ensures such violations in the way needed by local interpretations of quantum mechanics. I suggest that an as-yet unknown law or superselection rule may restrict the full tensor-product Hilbert space to the very special subspace required by the validity of records and the Past Hypothesis. Full article
(This article belongs to the Section Quantum Information)
14 pages, 5725 KiB  
Article
Study on Temperature Cascade ELM Inversion Method for 110 kV Single-Core Cable Intermediate Joints
by Xinhai Li, Bao Feng, Zhengang Wang, Jiangjun Ruan and Chang Xiao
Processes 2024, 12(5), 966; https://doi.org/10.3390/pr12050966 (registering DOI) - 9 May 2024
Abstract
The accurate calculation of the hotspot temperature of the cable intermediate joint can effectively guarantee the safe operation of the transmission and distribution network. This paper addresses the limitations of the current method of estimating hotspot temperature solely from surface temperature measurements. Specifically, [...] Read more.
The accurate calculation of the hotspot temperature of the cable intermediate joint can effectively guarantee the safe operation of the transmission and distribution network. This paper addresses the limitations of the current method of estimating hotspot temperature solely from surface temperature measurements. Specifically, we focus on a 110 kV single-core cable as our subject of study. We started by establishing a simulation model for the temperature field at the intermediate joint to generate data samples. Subsequently, the NCA (neighborhood component analysis) algorithm was employed to select the optimal measurement points on the cable’s surface. This allowed determination of the quantity and location of characteristic points. Finally, we developed a cascading inversion model, which consists of a radial inversion model and an axial inversion model, based on the extreme learning machine algorithm. The example results show that the mean squared error of hotspot temperature obtained by cascade inversion and direct inversion is 6.95 and 24.71, respectively, indicating that cascade inversion can effectively improve the inversion accuracy. Full article
(This article belongs to the Section Energy Systems)
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29 pages, 101396 KiB  
Article
When Two Eyes Don’t Suffice—Learning Difficult Hyperfluorescence Segmentations in Retinal Fundus Autofluorescence Images via Ensemble Learning
by Monty Santarossa, Tebbo Tassilo Beyer, Amelie Bernadette Antonia Scharf, Ayse Tatli, Claus von der Burchard, Jakob Nazarenus, Johann Baptist Roider and Reinhard Koch
J. Imaging 2024, 10(5), 116; https://doi.org/10.3390/jimaging10050116 (registering DOI) - 9 May 2024
Abstract
Hyperfluorescence (HF) and reduced autofluorescence (RA) are important biomarkers in fundus autofluorescence images (FAF) for the assessment of health of the retinal pigment epithelium (RPE), an important indicator of disease progression in geographic atrophy (GA) or central serous chorioretinopathy (CSCR). Autofluorescence images have [...] Read more.
Hyperfluorescence (HF) and reduced autofluorescence (RA) are important biomarkers in fundus autofluorescence images (FAF) for the assessment of health of the retinal pigment epithelium (RPE), an important indicator of disease progression in geographic atrophy (GA) or central serous chorioretinopathy (CSCR). Autofluorescence images have been annotated by human raters, but distinguishing biomarkers (whether signals are increased or decreased) from the normal background proves challenging, with borders being particularly open to interpretation. Consequently, significant variations emerge among different graders, and even within the same grader during repeated annotations. Tests on in-house FAF data show that even highly skilled medical experts, despite previously discussing and settling on precise annotation guidelines, reach a pair-wise agreement measured in a Dice score of no more than 63–80% for HF segmentations and only 14–52% for RA. The data further show that the agreement of our primary annotation expert with herself is a 72% Dice score for HF and 51% for RA. Given these numbers, the task of automated HF and RA segmentation cannot simply be refined to the improvement in a segmentation score. Instead, we propose the use of a segmentation ensemble. Learning from images with a single annotation, the ensemble reaches expert-like performance with an agreement of a 64–81% Dice score for HF and 21–41% for RA with all our experts. In addition, utilizing the mean predictions of the ensemble networks and their variance, we devise ternary segmentations where FAF image areas are labeled either as confident background, confident HF, or potential HF, ensuring that predictions are reliable where they are confident (97% Precision), while detecting all instances of HF (99% Recall) annotated by all experts. Full article
12 pages, 2962 KiB  
Article
Ultrasonic Pretreatment Combined with Microwave-Assisted Hydrodistillation for Extraction of Essential Oil from Melaleuca bracteata ‘Revolution Gold’ Leaves Scales Induced by Cellulase-Inorganic Salt and Its Anti-Fungal Activity
by Yan Huang, Xiaonan Zhang, Fajian Zeng, Jinmei Chang and Zhiwei Liu
Separations 2024, 11(5), 147; https://doi.org/10.3390/separations11050147 (registering DOI) - 9 May 2024
Abstract
In order to further develop the commercial use of Melaleuca bracteata (F. Muell), this report studied the extraction of essential oil from Melaleuca bracteata (F. Muell) leaves using ultrasonic pretreatment, cellulase-inorganic salt soaked and combined with microwave-assisted hydrodistillation. To optimize the primary contributing [...] Read more.
In order to further develop the commercial use of Melaleuca bracteata (F. Muell), this report studied the extraction of essential oil from Melaleuca bracteata (F. Muell) leaves using ultrasonic pretreatment, cellulase-inorganic salt soaked and combined with microwave-assisted hydrodistillation. To optimize the primary contributing parameters, the Box–Behnken design (BBD) was applied. The optimum yield of essential oil was 9.61 mL/kg DW at a microwave power of 510.77 W, lithium chloride dose of 63.56 μmol, and microwave irradiation period of 46.97 min. The essential oil included a total of 41 compounds, and methyl eugenol (76.53%) and methyl cinnamate (12.62%) were the main compounds. The inhibitory impact was notable when the essential oil concentration was 1.6 mg/mL. Therefore, it has the potential to replace chemical pesticides. When the concentration of the essential oil solution was 1.6 mg/mL, the three pathogenic species of fungus (Pseudocercospora psidii, Colletotrichum eriobotryae, and Colletotrichum siamense) were greatly affected; at this dose, the fungus was unable to develop and its growth diameter was 0 mm. Additionally, the fungus’s inhibition rate reached 100%. Full article
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19 pages, 3045 KiB  
Article
Effect of Metformin on Sertoli Cell Fatty Acid Metabolism and Blood–Testis Barrier Formation
by Gustavo Marcelo Rindone, Marina Ercilia Dasso, Cecilia Lucia Centola, Cristian Marcelo Sobarzo, María Noel Galardo, Silvina Beatriz Meroni and María Fernanda Riera
Biology 2024, 13(5), 330; https://doi.org/10.3390/biology13050330 (registering DOI) - 9 May 2024
Abstract
Sertoli cells (SCs) are essential to maintaining germ cell development. Metformin, the main pharmacologic treatment for pediatric type 2 diabetes, is administered to children during SC maturation. The present study aimed to analyze whether metformin affects SC energy metabolism and blood–testis barrier (BTB) [...] Read more.
Sertoli cells (SCs) are essential to maintaining germ cell development. Metformin, the main pharmacologic treatment for pediatric type 2 diabetes, is administered to children during SC maturation. The present study aimed to analyze whether metformin affects SC energy metabolism and blood–testis barrier (BTB) integrity. Primary SC cultures were used for the in vitro studies. In vivo effects were studied in Sprague–Dawley rats treated with 200 mg/kg metformin from Pnd14 to Pnd30. Metformin decreased fatty acid oxidation and increased 3-hydroxybutyrate production in vitro. Moreover, it decreased the transepithelial electrical resistance across the monolayer and induced ZO-1 redistribution, suggesting an alteration of cell junctions. In vivo, a mild but significant increase in BTB permeability and ZO-1 expression was observed in the metformin group, without changes in testicular histology and meiosis progression. Additionally, adult rats that received metformin treatment during the juvenile period showed no alteration in BTB permeability or daily sperm production. In conclusion, metformin exposure may affect BTB permeability in juvenile rats, but this seems not to influence spermatogenesis progression. Considering the results obtained in adult animals, it is possible to speculate that metformin treatment during the juvenile period does not affect testicular function in adulthood. Full article
(This article belongs to the Special Issue Germ, Sertoli and Leydig Cells in the Testis)
22 pages, 1294 KiB  
Article
Coexisting in the Surf Zone: Age and Feeding Habits of the Spotted Seabass (Dicentrarchus punctatus) and European Seabass (Dicentrarchus labrax) on the Gulf of Cádiz Beaches (Southwest Iberian Peninsula)
by Carlos Rodríguez-García, Ana Toro-Podadera, Jesica Sarmiento-Carbajal and Remedios Cabrera-Castro
Fishes 2024, 9(5), 173; https://doi.org/10.3390/fishes9050173 (registering DOI) - 9 May 2024
Abstract
Various commercial fish species utilize different coastal habitats as nurseries in their juvenile stages, with surf zones being particularly crucial due to the protection and abundant food resources they offer. Among the species that rely on these areas are the spotted seabass ( [...] Read more.
Various commercial fish species utilize different coastal habitats as nurseries in their juvenile stages, with surf zones being particularly crucial due to the protection and abundant food resources they offer. Among the species that rely on these areas are the spotted seabass (Dicentrarchus punctatus) and the European seabass (Dicentrarchus labrax). This study aimed to investigate the age and feeding habits of surf zone specimens of these species and explore their environmental adaptations. The average size for D. punctatus was 16.94 ± 4.05 cm, and for D. labrax, it was 23.23 ± 6.30 cm. The length–weight relationship for D. punctatus was TW = 0.013*TL2.885 (parameter a: 95% CI: 0.012–0.014; parameter b: 95% CI: 2.854–2.917), and for D. labrax, it was TW = 0.008*TL3.095 (parameter a: 95% CI: 0.006–0.009; parameter b: 95% CI: 3.040–3.151). In both D. punctatus (79.20%) and D. labrax (75.92%), the predominant age classes were 1+ and 2+. Significant variations in age class abundance were observed in D. punctatus based on the time of day, lunar phase, and season, while D. labrax showed variations only by season. A diet analysis revealed that both species primarily consume mysids and fish, with D. punctatus showing dietary variations related to the time of day, lunar phase, and season, and D. labrax showing variations with the time of day and season. Full article
(This article belongs to the Section Biology and Ecology)
20 pages, 7002 KiB  
Article
Optimizing Hadoop Scheduling in Single-Board-Computer-Based Heterogeneous Clusters
by Basit Qureshi
Computation 2024, 12(5), 96; https://doi.org/10.3390/computation12050096 (registering DOI) - 9 May 2024
Abstract
Single-board computers (SBCs) are emerging as an efficient and economical solution for fog and edge computing, providing localized big data processing with lower energy consumption. Newer and faster SBCs deliver improved performance while still maintaining a compact form factor and cost-effectiveness. In recent [...] Read more.
Single-board computers (SBCs) are emerging as an efficient and economical solution for fog and edge computing, providing localized big data processing with lower energy consumption. Newer and faster SBCs deliver improved performance while still maintaining a compact form factor and cost-effectiveness. In recent times, researchers have addressed scheduling issues in Hadoop-based SBC clusters. Despite their potential, traditional Hadoop configurations struggle to optimize performance in heterogeneous SBC clusters due to disparities in computing resources. Consequently, we propose modifications to the scheduling mechanism to address these challenges. In this paper, we leverage the use of node labels introduced in Hadoop 3+ and define a Frugality Index that categorizes and labels SBC nodes based on their physical capabilities, such as CPU, memory, disk space, etc. Next, an adaptive configuration policy modifies the native fair scheduling policy by dynamically adjusting resource allocation in response to workload and cluster conditions. Furthermore, the proposed frugal configuration policy considers prioritizing the reduced tasks based on the Frugality Index to maximize parallelism. To evaluate our proposal, we construct a 13-node SBC cluster and conduct empirical evaluation using the Hadoop CPU and IO intensive microbenchmarks. The results demonstrate significant performance improvements compared to native Hadoop FIFO and capacity schedulers, with execution times 56% and 22% faster than the best_cap and best_fifo scenarios. Our findings underscore the effectiveness of our approach in managing the heterogeneous nature of SBC clusters and optimizing performance across various hardware configurations. Full article
(This article belongs to the Section Computational Engineering)
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14 pages, 3177 KiB  
Article
Comparative Study on the Determination of Chlorophyll-a in Lake Phytoplankton by a YSI Multi-Parameter Water Quality Meter and Laboratory Spectrophotometric Method
by Jie Wang, Lizeng Duan, Donglin Li, Yuwei Zhang, Zheng Yuan, Huayu Li and Hucai Zhang
Water 2024, 16(10), 1350; https://doi.org/10.3390/w16101350 (registering DOI) - 9 May 2024
Abstract
Algal blooms caused by eutrophication are a major global problem, and the monitoring and prediction of algal densities in lakes are important indicators of eutrophication management. However, the reliability of the commonly used chlorophyll-a (Chl-a) to characterize phytoplankton density in [...] Read more.
Algal blooms caused by eutrophication are a major global problem, and the monitoring and prediction of algal densities in lakes are important indicators of eutrophication management. However, the reliability of the commonly used chlorophyll-a (Chl-a) to characterize phytoplankton density in lake environments needs to be further investigated. In this paper, we sampled and analyzed 365 samples from nine plateau lakes in Yunnan Province during the dry and rainy seasons. The Chl-a data measured by the laboratory spectrophotometric method and the portable YSI multi-parameter water quality meter (YSI) directly used in the field were compared, and regression analysis and correlation analysis with phytoplankton density were performed. Most of the Chl-a values measured by the laboratory instrument were greater than those measured by the YSI, and the correlation between the two methods was weak (0.492, p < 0.001). The correlation between Chl-a and phytoplankton density measured by the YSI reached 0.67 (p < 0.001) in the dry season, while the laboratory methods used to measure Chl-a to characterize phytoplankton density may have led to an overestimation of phytoplankton density due to nonspecific sources of Chl-a. However, both methods are relatively inaccurate for characterizing phytoplankton density. For different trophic states of lakes, nutrient concentration changes affect the Chl-a concentration of phytoplankton. During different seasons, changes in the fluorescence intensity of phytoplankton in response to environmental conditions prevent the YSI results from reflecting the authentic phytoplankton density. Furthermore, high species diversity can lead to inconsistent changes in Chl-a and phytoplankton because the content of Chl-a in individual cells of different phytoplankton is different. The relationship between Chl-a and phytoplankton density was species specific. Therefore, when applying Chl-a to characterize phytoplankton density in lakes, it is necessary to consider environmental conditions, phytoplankton community structure and other practical conditions. In addition, laboratory analytical methods and instrumental techniques and instruments need to be improved. Full article
16 pages, 2068 KiB  
Article
Comparative Study of Systemic vs. Local Antibiotics with Subgingival Instrumentation in Stage III–IV Periodontitis: A Retrospective Analysis
by Ioana Ilyes, Marius Boariu, Darian Rusu, Vincenzo Iorio-Siciliano, Octavia Vela, Simina Boia, Viorelia Radulescu, Petra Șurlin, Holger Jentsch, Alexandru Lodin and Stefan-Ioan Stratul
Antibiotics 2024, 13(5), 430; https://doi.org/10.3390/antibiotics13050430 (registering DOI) - 9 May 2024
Abstract
To improve the clinical and microbiological outcomes of non-surgical mechanical periodontal therapy, the adjunctive use of antimicrobials has been utilized in treating moderate-to-severe periodontitis. In our study, the retrospective design included previously collected health-related patient data, obtained from the printed and digital charts [...] Read more.
To improve the clinical and microbiological outcomes of non-surgical mechanical periodontal therapy, the adjunctive use of antimicrobials has been utilized in treating moderate-to-severe periodontitis. In our study, the retrospective design included previously collected health-related patient data, obtained from the printed and digital charts of patients who received systemic or local antibiotic adjuncts to SI (subgingival instrumentation). A total of 34 patients (diagnosed with generalized Stage III/IV periodontitis) met the inclusion and exclusion criteria and were evaluated. The samples were tested for the following bacterial strains: Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), Porphyromonas gingivalis (P. gingivalis), Prevotella intermedia (P. intermedia), Tanererella forsythia (T. forsythia), and Treponema denticola (T. denticola). The inter-group comparisons of the bacterial species did not show statistically significant differences between groups. The present study aimed to evaluate the clinical effects after SI and the adjunctive use of systemically administered (SA) AMX (amoxicillin) + MET (metronidazole) (administered for 7 days), with locally delivered (LDD) piperacillin + tazobactam in step 2 of periodontal therapy. Results: Overall, all parameters were improved in the groups, with a significant difference in inter-group comparison regarding the full-mouth bleeding score (FMBS) (p < 0.05) in favor of the SA group, and the p-value < 0.05 was considered to be statistically significant. Statistically significant PPD (probing pocket depth) reductions and CAL (clinical attachment level) gains were observed in both groups at the 3-month follow-up. In conclusion, within the limitations, the outcomes of this study suggest that SI, with adjunctive local or systemic antibiotic therapy, provided comparable clinical improvements. Systemic AMX + MET protocols were more efficacious with regard to the reduction in FMBS. Follow-up studies with larger patient numbers are needed to further investigate this effect. Full article
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14 pages, 5419 KiB  
Article
Evaluation of Sedimentary Characteristics of the Chang 9 Oil Layer Formation in the Yanchang Formation, Ordos Basin
by Xuelin Qiu and Yaning Wang
Appl. Sci. 2024, 14(10), 4035; https://doi.org/10.3390/app14104035 (registering DOI) - 9 May 2024
Abstract
Shallow-water deltas are a subject of sedimentary research and represent a significant target for oil and gas exploration. The Yanchang Formation of the Triassic in the Ordos Basin comprises numerous shallow-water delta blocks. This paper addresses the core issues pertaining to the sedimentary [...] Read more.
Shallow-water deltas are a subject of sedimentary research and represent a significant target for oil and gas exploration. The Yanchang Formation of the Triassic in the Ordos Basin comprises numerous shallow-water delta blocks. This paper addresses the core issues pertaining to the sedimentary facies, sedimentary characteristics and sand body distribution of the Chang 9 oil layer formation of the Upper Triassic in the Ordos Basin. Guided by the relevant theories and methods of contemporary sedimentology and sedimentary geology, the reservoir characteristics are described and studied in detail through ordinary thin sections, cast thin sections, graphical representations of particle size and scanning electron microscopy experiments. The experimental results indicate that the porosity in the study area ranges from 3% to 12% and that the permeability is between 0 and 1.5 × 10−3 μm2, which is consistent with classification as an ultra-low-porosity and ultra-low-permeability reservoir. The Chang 9 sandstone is composed of feldspar sandstone and lithic feldspar sandstone. The average content of quartz is low, at less than 31%, while the average content of feldspar is high, at more than 34%. The average content of rock debris is between 10% and 20%. Therefore, the compositional maturity of the Chang 9 sand body is generally low. The particle size distribution exhibits a positive deviation, indicating that the sediments in the sand body are primarily coarse-grained components. The kurtosis of the particle size–frequency curve is observed to vary from flat to very sharp. The Chang 91 lake is classified as a shore shallow lake with basin subsidence and lake transgression. The Chang 9 period saw the development of the Chang 91 sedimentary facies into a semi-deep lake–deep lake environment. The vertical structural style of the Chang 9 oil layer formation in the basin can be roughly summarized into three basic structural types: the sedimentary structures observed in the area include box-shaped upward thinning, bell-shaped upward thinning and funnel-shaped upward thickening. The delta front area in Chang 9 is notable for its size and the prevalence of underwater distributary channel microfacies. The sand body distribution is stable, with sand layer thicknesses ranging from 15 to 30 m. The evaluation and summary of the sedimentary characteristics of the Chang 9 oil layer formation provide a geological basis for future exploration and development in the study area. Full article
(This article belongs to the Section Earth Sciences)
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20 pages, 18357 KiB  
Article
Mechanical Fault Diagnosis of High-Voltage Circuit Breakers with Dynamic Multi-Attention Graph Convolutional Networks Based on Adaptive Graph Construction
by Guoqing Sui, Jing Yan, Yanze Wu, Zhuofan Xu, Meirong Qi and Zilong Zhang
Appl. Sci. 2024, 14(10), 4036; https://doi.org/10.3390/app14104036 (registering DOI) - 9 May 2024
Abstract
With the rapid development of deep learning, its powerful capabilities make it possible to perform mechanical fault diagnosis of high-voltage circuit breakers (HVCBs). Among deep learning approaches, the convolutional neural network is widely used. However, while it can extract features effectively, it also [...] Read more.
With the rapid development of deep learning, its powerful capabilities make it possible to perform mechanical fault diagnosis of high-voltage circuit breakers (HVCBs). Among deep learning approaches, the convolutional neural network is widely used. However, while it can extract features effectively, it also has some limitations. Specifically, it depends on a large number of training data and only takes data information into account without considering structural information. These shortcomings lead to unused information and unsatisfactory model results. To address these shortcomings, this paper proposes AKNN-DMGCN, a novel dynamic multi-attention graph convolutional network based on an adaptively constructed graph, which can achieve high accuracy and robust mechanical fault diagnosis of HVCBs. First, a novel adaptive k-nearest neighbor (AKNN) graph construction method is proposed to construct informative graphs. The AKNN method can mine the relationship between the original data samples and utilize the data and label information. Thus, it has high fault tolerance to noise signals and can construct a structure graph with rich and accurate information, which can improve the overall model performance. Then, a dynamic multi-attention graph convolutional network (DMGCN) is applied for mechanical fault diagnosis of HVCBs. DMGCN fully utilizes structural and numerical information representing HVCB signals to perform classification. DMGCN has a dynamic multi-attention mechanism with strong expressive ability, which allows it to achieve high diagnostic accuracy. The experimental results indicate that the accuracy of AKNN-DMGCN reaches 97.22% on a balanced dataset and 95.01% on an imbalanced dataset, which demonstrates that the proposed method is effective for both balanced and imbalanced samples. Full article
(This article belongs to the Section Electrical, Electronics and Communications Engineering)
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28 pages, 811 KiB  
Review
Pulse Consumption and Health Outcomes: A Scoping Review
by Naisi Zhao, Keyi Jiao, Yu-Hsiang Chiu and Taylor C. Wallace
Nutrients 2024, 16(10), 1435; https://doi.org/10.3390/nu16101435 (registering DOI) - 9 May 2024
Abstract
Pulses—comprising the dry, edible seeds of leguminous plants—have long been lauded for their culinary flexibility and substantial nutritional advantages. This scoping review aimed to map the evidence on how pulses contribute to overall human health. Four electronic databases were searched for clinical and [...] Read more.
Pulses—comprising the dry, edible seeds of leguminous plants—have long been lauded for their culinary flexibility and substantial nutritional advantages. This scoping review aimed to map the evidence on how pulses contribute to overall human health. Four electronic databases were searched for clinical and observational studies in English. We identified 30 articles (3 cross-sectional studies, 1 federated meta-analysis, 8 prospective cohort studies, 1 before-and-after study, and 17 randomized controlled trials) that met our inclusion criteria. Predominant among the pulses studied were lentils, chickpeas, common bean varieties (e.g., pinto, black, navy, red, kidney), black-eyed peas, cowpeas, and split peas. Consumption modalities varied; most studies examined mixed pulses, while five isolated individual types. In intervention studies, pulses were incorporated into diets by allotting a fixed pulse serving on top of a regular diet or by substituting red meat with pulses, offering a comparative analysis of dietary effects. The health outcomes evaluated were multifaceted, ranging from lipid profiles to blood pressure, cardiovascular disease risk and mortality, type 2 diabetes and glycemic control, metabolic syndrome indicators, inflammatory markers, oxidative stress biomarkers, and hormonal profiles. The most frequently assessed study outcomes included changes in low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, systolic blood pressure, diastolic blood pressure, fasting blood sugar, hemoglobin A1c, waist circumference, and C-reactive protein or high-sensitivity C-reactive protein. This review should serve as a call to action for the scientific community to build upon the existing evidence, enriching our understanding of the nutritional and health-promoting attributes of pulses. Full article
(This article belongs to the Section Nutrition and Public Health)
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25 pages, 30172 KiB  
Article
Comprehensive Analysis on GPS Carrier Phase under Various Cutoff Elevation Angles and Its Impact on Station Coordinates’ Repeatability
by Sorin Nistor, Norbert-Szabolcs Suba, Aurelian Stelian Buda, Kamil Maciuk and Ahmed El-Mowafy
Remote Sens. 2024, 16(10), 1691; https://doi.org/10.3390/rs16101691 (registering DOI) - 9 May 2024
Abstract
When processing the carrier phase, the global navigation satellite system (GNSS) grants the highest precision for geodetic measurements. The analysis centers (ACs) from the International GNSS Service (IGS) provide different data such as precise clock data, precise orbits, reference frame, ionosphere and troposphere [...] Read more.
When processing the carrier phase, the global navigation satellite system (GNSS) grants the highest precision for geodetic measurements. The analysis centers (ACs) from the International GNSS Service (IGS) provide different data such as precise clock data, precise orbits, reference frame, ionosphere and troposphere data, as well as other geodetic products. Each individual AC has its own strategy for delivering the abovementioned products, with one of the key elements being the cutoff elevation angle. Typically, this angle is arbitrarily chosen using generic values without studying the impact of this choice on the obtained results, in particular when very precise positions are considered. This article addresses this issue. To this end, the article has two key sections, and the first is to evaluate the impact of using the two different cutoff elevation angles that are most widely used: (a) 3 degrees cutoff and (b) 10 degrees cutoff elevation angle. This analysis is completed in two major parts: (i) the analysis of the root mean square (RMS) for the carrier phase and (ii) the analysis of the station position in terms of repeatability. The second key section of the paper is a comprehensive carrier phase analysis conducted by adopting a new approach using a mean of the 25-point average RMS (A-RMS) and the single-point RMS and using an ionosphere-free linear combination. By using the ratio between the 25-point average RMS and the single-point RMS we can define the type of scatter that dominates the phase solution. The analyzed data span a one-year period. The tested GNSS stations belong to the EUREF Permanent Network (EPN) and the International GNSS Service (IGS). These comprise 55 GNSS stations, of which only 23 GNSS stations had more than 95% data availability for the entire year. The RMS and A-RMS are analyzed in conjunction with the precipitable water vapor (PWV), which shows clear signs of temporal correlation. Of the 23 GNSS stations, three stations show an increase of around 50% of the phase RMS when using a 3° cutoff elevation angle, and only four stations have a difference of 5% between the phase RMS when using both cutoff elevation angles. When using the A-RMS, there is an average improvement of 37% of the phase scatter for the 10° cutoff elevation angle, whereas for the 3° cutoff elevation angle, the improvement is around 33%. Based on studying this ratio, four stations indicate that the scatter is dominated by the stronger-than-usual dominance of long-period variations, whereas the others show short-term noise. In terms of station position repeatability, the weighted root mean square (WRMS) is used as an indicator, and the results between the differences of using a 3° and 10° cutoff elevation angle strategy show a difference of −0.16 mm for the North component, −0.21 mm for the East component and a value of −0.75 mm for the Up component, indicating the importance of using optimal cutoff angles. Full article
(This article belongs to the Special Issue Advanced Remote Sensing Technology in Modern Geodesy)
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18 pages, 7964 KiB  
Article
Operational Reliability Analysis of Turbine Blisk Using an Enhanced Moving Neural Network Framework
by Xiao Liang, Wei Sun, Qingchao Sun and Chengwei Fei
Aerospace 2024, 11(5), 382; https://doi.org/10.3390/aerospace11050382 (registering DOI) - 9 May 2024
Abstract
As one of the key components of an aeroengine, turbine blisk endures complex coupling loads under a harsh operational environment so that the reliability of turbine blisk directly influences the safe operation of aeroengine. It is urgent to precisely perform the reliability estimation [...] Read more.
As one of the key components of an aeroengine, turbine blisk endures complex coupling loads under a harsh operational environment so that the reliability of turbine blisk directly influences the safe operation of aeroengine. It is urgent to precisely perform the reliability estimation of a complex blisk structure. To address this issue, an enhanced Moving Neural Network Framework (MNNF) is proposed by integrating compact support region theory, improve sooty tern optimization algorithm (ISTOA), and Bayesian regularization strategy into artificial neural network. The compact support region theory is applied to select the efficient samples for modeling from the training samples set, the ISTOA is to determine the optimal compact support region, and Bayesian regularization thought is utilized to improve the generalization ability of neural network model. The operational reliability assessment of aeroengine blisk is performed with the consideration of transient loads to verify the proposed MNNF method. It is shown that the reliability degree of turbine blisk stain is 0.9984 when the allowable value is 5.2862 × 10−3 m. In line with the comparison of methods, the developed MNNF approach has 0.99738 in root means square error, 3.1634 × 10−4 m in goodness of fit, 0.423 s in modeling time, 99.99% in simulation precision, and 0.496 s in simulation time under 10,000 simulations, which are superior to all other methods (i.e., 99.96%, 99.91%, 99.93%, 99.97%, and 99.97% in simulation precision and 16.27%, 4.82%, 30.07%, 39.87%, and 23.59% in simulation efficiency, for the response surface method (RSM), Kriging, support vector machine (SVM), back propagation-artificial neural network (BP-NN), and BP-NN based on particle swarm optimization (BP-PSO) methods, respectively). It is demonstrated that the MNNF method holds excellent modeling and simulation performances. The efforts of this study provide promising tools and insights into the reliability design of complex structures, and enrich and develop reliability theory. Full article
(This article belongs to the Section Aeronautics)
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