This study investigates data aggregation bias in estimating market power in the U.S. beef packing industry using New Empirical Industrial Organization (NEIO) models and shows empirical procedures that can alleviate the bias. Unlike many earlier studies in estimating market power exertion, our study examines the data aggregation bias when market-level data are used in place of firm-level data and show how the bias could be reduced. We first derive data aggregation bias analytically, then empirically investigate the aggregation bias by estimating both firm and aggregate industry models. Because the firm-level data are not available, we use simulated data generated from the Monte Carlo simulation method. Hybrid models, combining limited firm-level data with aggregate data, are also estimated to illustrate how the aggregation bias could be reduced. Our results show that aggregate models with industry-level data tend to underestimate market power exertion in the U.S. beef packing industry, and the aggregation bias is statistically significant at the 1% level. Comparing results from hybrid models with firm-level estimates, we find that hybrid models reduce the bias but do not remove the aggregation bias significantly. The sensitivity analysis shows that market power estimate and aggregation bias are sensitive to functional forms. Full article

Age-related macular degeneration (AMD) is an age-related disorder that is a global public health problem. The non-enzymatic Maillard reaction results in the formation of advanced glycation end products (AGEs). Accumulation of AGEs in drusen plays a key role in AMD. AGE-reducing drugs may contribute to the prevention and treatment of AGE-related disease. Fructosamine oxidase (FAOD) acts on fructosyl lysine and fructosyl valine. Based upon the published results of fructosamine 3-kinase (FN3K) and FAOD obtained in cataract and presbyopia, we studied ex vivo FAOD treatment as a non-invasive AMD therapy. On glycolaldehyde-treated porcine retinas, FAOD significantly reduced AGE autofluorescence (p = 0.001). FAOD treatment results in a breakdown of AGEs, as evidenced using UV fluorescence, near-infrared microspectroscopy on stained tissue sections of human retina, and gel permeation chromatography. Drusen are accumulations of AGEs that build up between Bruch’s membrane and the retinal pigment epithelium. On microscopy slides of human retina affected by AMD, a significant reduction in drusen surface to 45 ± 21% was observed following FAOD treatment. Enzymatic digestion followed by mass spectrometry of fructose- and glucose-based AGEs (produced in vitro) revealed a broader spectrum of substrates for FAOD, as compared to FN3K, including the following: fructosyllysine, carboxymethyllysine, carboxyethyllysine, and imidazolone. In contrast to FN3K digestion, agmatine (4-aminobutyl-guanidine) was formed following FAOD treatment in vitro. The present study highlights the therapeutic potential of FAOD in AMD by repairing glycation-induced damage. Full article

Galium genus belongs to the Rubiaceae family, which consists of approximately 14,000 species. In comparison to its well-known relatives, the plastomes of the Galium genus have not been explored so far. The plastomes of this genus have a typical, quadripartite structure, but differ in gene content, since the infA gene is missing in Galium palustre and Galium trfidum. An evaluation of the effectiveness of using entire chloroplast genome sequences as superbarcodes for accurate plant species identification revealed the high potential of this method for molecular delimitation within the genus and tribe. The trnE-UUC—psbD region showed the biggest number of diagnostides (diagnostic nucleotides) which might be new potential barcodes, not only in Galium, but also in other closely related genera. Relative synonymous codon usage (RSCU) appeared to be connected with the phylogeny of the Rubiaceae family, showing that during evolution, plants started preferring specific codons over others. Full article

Hurricanes Ian and Nicole caused devastating destruction across Florida in September and November 2022, leaving widespread damage in their wakes. This study focuses on the assessment of barrier islands’ shorelines, encompassing natural sand dunes and dune vegetation as well as armored dunes with man-made infrastructure such as seawalls. High-resolution satellite imagery from Planet was used to assess the impacts of these hurricanes on the beach shorelines of Volusia, Flagler, and St. Johns Counties on the Florida Central East Coast. Shorefront vegetation was classified into two classes. Normalized Difference Vegetation Index (NDVI) values were calculated before the hurricanes, one month after Hurricane Ian, one month after Hurricane Nicole, and one-year post landfall. LiDAR (Light Detection and Ranging) was incorporated to calculate vertical changes in the shorelines before and after the hurricanes. The results suggest that natural sand dunes were more resilient as they experienced less impact to vegetation and elevation and more substantial recovery than armored dunes. Moreover, the close timeframe of the storm events suggests a compound effect on the weakened dune systems. This study highlights the importance of understanding natural dune resilience to facilitate future adaptive management efforts because armored dunes may have long-term detrimental effects on hurricane-prone barrier islands. Full article

TR2 and TR4 (NR2C1 and NR2C2, respectively) are evolutionarily conserved nuclear orphan receptors capable of binding direct repeat sequences in a stage-specific manner. Like other nuclear receptors, TR2 and TR4 possess important roles in transcriptional activation or repression with developmental stage and tissue specificity. TR2 and TR4 bind DNA and possess the ability to complex with available cofactors mediating developmental stage-specific actions in primitive and definitive erythrocytes. In erythropoiesis, TR2 and TR4 are required for erythroid development, maturation, and key erythroid transcription factor regulation. TR2 and TR4 recruit and interact with transcriptional corepressors or coactivators to elicit developmental stage-specific gene regulation during hematopoiesis. Full article

Climate change significantly impacts the livelihoods of farmer households. Particularly vulnerable areas, both economically and environmentally, face significant threats from climate change. This study developed a framework to assess household-level vulnerability to climate change by integrating the Exposure-Sensitivity-Resilience Analysis (ESRA) and Sustainable Livelihoods Analysis (SLA) frameworks. Using Gui-Zhou Province as the study area, the study examined whether livelihood vulnerability differs among various types of farmer households in economically and environmentally vulnerable areas and identified the main factors contributing to vulnerability. Results indicate significant differences in livelihood vulnerability among the three household types, with pure agricultural households (PAH) being the most vulnerable due to high exposure, sensitivity, and low adaptive capacity. Further analysis revealed minor differences in sensitivity but significant differences in adaptive capacity among the three farmer categories. In terms of sensitivity, all three farmer household categories exhibit high sensitivity to water, housing, and agricultural production. Regarding adaptive capacity, significant differences in human and financial capital exist among the three household types, with off-farm households (OFH) possessing the highest adaptive capacity due to their substantial human and financial capital. Further research identified high exposure and low adaptive capacity as the primary causes of livelihood vulnerability, noting no significant difference in the main contributing factors among the three types of farmer households. Common factors contributing to the livelihood vulnerability of farmer households include agricultural cooperatives, labor capacity, temperature changes, drought frequency changes, precipitation changes, agricultural insurance, and losses in agricultural production. Overall, the proposed livelihood vulnerability framework offers guidance for analyzing the vulnerability of farmer households in areas with both economic and environmental vulnerabilities under climate change. Concurrently, tailored measures to reduce farmer households’ livelihood vulnerability should be developed for different household types, considering the local climatic, geographic, and socioeconomic conditions. Full article

Simultaneous interrogation of pump and probe beams interacting in ZnO nanostructures of a two-wave mixing is proposed for dual-path data processing of optical signals by nonlinear optical effects. An enhancement in third-order nonlinear optical properties was exhibited by Al-doped ZnO thin films. Multiphoton absorption and nonlinear refraction were explored by the z-scan technique at 532 nm with nanosecond pulses. The evolution of the optical Kerr effect in the ZnO thin films was analyzed as a function of the incorporation of Al in the sample by a vectorial two-wave mixing method. Electrical and photoconductive effects were evaluated to further characterize the influence of Al in the ZnO solid samples. Potential applications of nonlinear optical parameters for encoding and encrypting information in light can be envisioned. Full article

Snow and ice melting in the Upper Indus Basin (UIB) is crucial for regional water availability for mountainous communities. We analyzed glacier changes in the Astak catchment, UIB, from 2000 to 2020 using remote sensing techniques based on optical satellite images from Landsat and ASTER digital elevation models. We used a surface feature-tracking technique to estimate glacier velocity. To assess the impact of climate variations, we examined temperature and precipitation anomalies using ERA5 Land climate data. Over the past two decades, the Astak catchment experienced a slight decrease in glacier area (−1.8 km²) and the overall specific mass balance was −0.02 ± 0.1 m w.e. a⁻¹. The most negative mass balance of −0.09 ± 0.06 m w.e. a⁻¹ occurred at elevations between 2810 to 3220 m a.s.l., with a lesser rate of −0.015 ± 0.12 m w.e. a⁻¹ above 5500 m a.s.l. This variation in glacier mass balance can be attributed to temperature and precipitation gradients, as well as debris cover. Recent glacier mass loss can be linked to seasonal temperature anomalies at higher elevations during winter and autumn. Given the reliance of mountain populations on glacier melt, seasonal temperature trends can disturb water security and the well-being of dependent communities. Full article

The integration of arts-based methods into nursing education is a topic of growing interest in nursing practice. While there is an emerging body of research on this subject, evidence on competence development remains vague, largely due to methodological weaknesses. The purpose of this review is to evaluate the effectiveness of arts-based pedagogy in nursing, specifically in terms of students’ changes in knowledge, skills, and attitudes. It explores which arts-based approaches to nursing education qualify as evidence-based practice in terms of nursing competence. A systematic critical review of research on arts-based pedagogy in nursing was conducted, identifying 43 relevant studies. These studies were assessed for methodological quality based on the CEC Standards for evidence-based practice, and 13 high-quality comparative studies representing a variety of arts-based approaches were selected. Creative drama was identified as the only evidence-based practice in the field, positively affecting empathy. The findings highlight a research gap in nursing education and emphasize the need for measurement and appraisal tools suitable for the peculiarities of arts-based pedagogy. Full article

In this paper, we provide a collocation spectral scheme for systems of nonlinear Caputo–Hadamard differential equations. Since the Caputo–Hadamard operators contain logarithmic kernels, their solutions can not be well approximated using the usual spectral methods that are classical polynomial-based schemes. Hence, we construct a non-polynomial spectral collocation scheme, describe its effective implementation, and derive its convergence analysis in both $L^2$ and $L^{\infty }$. In addition, we provide numerical results to support our theoretical analysis. Full article

The paper presented delivers the proof for one of the possible solutions to the so-called medium-temperature gap—the lack of electrolytic systems able to efficiently work in a temperature range spanning from 200 to 450 °C. Regardless of the progress made in this field, the commercially available systems are still operating either at close to ambient temperatures, where hydrogen purity requirements are a significant limit, or above ca. 600 °C, where they suffer from increased corrosion and excessive thermal stresses occurring during startup and shutdown. Alkali metal orthoborates (M₃BO₃ M = Li, Na, K, or the mixture of these), in contrast to commercially used tetra-(M₂B₄O₇) and meta-(MBO₂) borates of these metals, are compounds with relatively poorly understood structure and physicochemical properties. The possibility of their application as an electrolyte in a fuel cell is a relatively new idea and has been preliminary reported. Therefore, an extended phase-focused analysis of the materials applied was needed to re-optimize both the synthetic strategy and the application route. Results of PXRD and FT-IR investigations showed, on the one hand, a complicated multi-phase structure, including the main orthoborate phase, as well as the presence of additional borate-based phases, including boric oxoacid. On the other hand, DTA tests proved not only that their melting temperatures are lower than these characteristics for the tetra- and meta-counterparts, but also that cation mixing leads to a subsequent decrease in this important functional parameter of the materials studied. Full article

To reduce the power consumption of a TDC in high-speed applications, a TDC architecture applied to SS ADC is proposed to reduce redundant counting. This structure can remove the identical part between two rows of pixel signals in a CMOS image sensor by adjusting the start and stop signal of the TDC, which will reduce the number of flipping of D flip-flops in the TDC. This structure requires the simultaneous readout of two rows of pixels in the high-speed CMOS image sensor. In the 110 nm CMOS process, simulation results show that the designed 5-bit TDC achieves an effective number of bits (ENOB) at 4.72 bits and a figure-of-merit (FOM) at 104.7–162.3 fJ/step, with a power consumption ranging from 60 µW to 93 µW. Compared with traditional counting methods, the proposed TDC can reduce counting power consumption by 30%. Full article

Plasminogen activators, such as recombinant tissue-type plasminogen activators (rtPAs), while effective in treating thromboembolic diseases, often induce hemorrhagic complications due to non-specific enzyme activities in the systemic circulation. This study evaluated the targeting efficiency, efficacy, biodistribution, and potential toxicity of a rtPA covalently attached to chitosan-coated magnetic nanoparticles (chitosan-MNP-rtPA). The thrombolytic activity of a chitosan-MNP-rtPA was preserved by protection from an endogenous plasminogen activator inhibitor-1 (PAI-1) in whole blood and after circulation in vivo, as examined by thromboelastometry. Single-photon emission computed tomography (SPECT) demonstrated real-time retention of a ^99mTc-MNP-rtPA induced by magnet application in a rat embolic model; an 80% reduction in rtPA dosage for a chitosan-MNP-rtPA with magnetic guidance was shown to restore blood flow. After treatment, iron deposition was observed in the reticuloendothelial systems, with portal edema and neutrophil infiltration in the liver at a ten-fold higher dose but not the regular dose. Nevertheless, no liver or renal toxicity was observed at this higher dose. In conclusion, the liver may still be the major deposit site of rtPA nanocomposites after targeted delivery; chitosan-coated MNPs are potentially amenable to target therapeutics with parenteral administration. Full article

Alginate films plasticized with glycerol and enriched in raspberry and/or black currant seed oils were prepared via casting solution techniques. The intention was to create active films for food packaging where antioxidants in a film would deactivate oxidants in a packed product or its surroundings, improving conditions inside packaging and extending the shelf life of such a product. The prepared materials were characterized by physicochemical, spectroscopic, mechanical, water vapor transmission (WVTR), and antioxidant activity analysis. Infrared spectra of the alginate films with oils were similar to those without the additive; the band with a maximum at about 1740 cm⁻¹ stood out. The prepared materials with oils were thicker, contained less water, were more yellow, and were less permeable to water vapor. Moreover, the presence of the oil in the films resulted in a slightly lower Young’s modulus and lower stress at break values but higher strain at break. The antioxidant capacity of raspberry seed oil itself was about five times higher than that of black currant seed oil, and a similar trend was noticed for films modified with these oils. The results indicated that both oils could be used as active substances with antioxidant properties in food packaging. Full article

Growing concern over antimicrobial resistance in chronic wound patients necessitates the exploration of alternative treatments from natural sources. This study suggests that honey’s phenolic compounds may offer antimicrobial benefits, warranting further investigation for therapeutic development. The main aim of this study was to investigate the antimicrobial activity of phenolic compounds and to determine the effects of their sub-inhibitory concentrations against Escherichia coli (E. coli). 3-phenyllactic acid (PLA), p-coumaric acid (PCA), and phloretin were tested against the bacterial strain of E. coli ATCC 25922. Comparison of the antimicrobial activity of honey constituents in vitro was performed using a broth culture assay. Measurement of the inhibitory properties of constituents in vitro was conducted using disc and well diffusion assays. The effects of sub-inhibitory concentrations of PCA on the susceptibility of E. coli ATCC 25922 to penicillin–streptomycin were tested. The results demonstrated that PLA was the most efficient antimicrobial agent, followed by PCA, whereas phloretin, at lower (2 mg/mL) concentrations, led to an increase in the growth of E. coli. Various modifications of the agar diffusion assay did not reveal the antibacterial properties of the studied phytochemicals. The enhancing effect of a sub-inhibitory concentration of PCA in cooperation with penicillin–streptomycin was shown. These findings might be helpful for the further investigation and development of new antimicrobial agents for the treatment of skin infections and wounds. Full article

Coxiellosis or Q fever is an infectious zoonotic disease caused by the bacterium Coxiella burnetii. A systematic review using bibliographic research was carried out, and the focus was the relationship between C. burnetii infection and reproductive disorders in cattle [abortion/stillbirth/perinatal morality/weak calves (ASPW complex); retained foetal membranes (RFMs); metritis/endometritis; and infertility/sub-fertility]. The bibliographical search yielded 443 results from databases, but only 61 were deemed eligible. For each disorder, summary tables were prepared, and a scientific evidence score was calculated for each study based on four criteria to help assess the level of evidence for the impact of C. burnetii on the reproductive disorders assessed: type of publication (peer-reviewed or other); type of study (case–control/cohort or other); type of C. burnetii test (direct or indirect); and comparative statistical analysis (yes or no). In addition, summary tables also included information on the study population, country, authors and year of publication, key findings and an assessment of the evidence for an association. For the ASPW complex, RFMs, metritis/endometritis and infertility/sub-fertility, 43, 9, 8 and 19 studies provided data, respectively. On a scale of four, nearly 50% of all study citations had evidence scores of three or four. For ASPW, RFMs and infertility/sub-fertility, there is a significant body of evidence to support a deleterious role for Q fever. In contrast, for metritis/endometritis, the evidence is unclear. It is concluded that there is a substantial need for further research, particularly involving larger animal populations in more controlled settings. To provide more consistency, it is recommended that authors follow more precise definitions of reproductive parameters and more robust diagnostic methodologies. Full article

Shikonin is a naphthoquinone pigment present in the hairy roots of the plant species from the Boraginaceae family. The compound has been well investigated for its highly efficient medicinal, antioxidant, and antimicrobial properties. Various extraction methodologies have been employed to maximise yield while minimising waste production of shikonin and its derivatives. Despite substantial research on shikonin and Boraginaceae plants, a research gap persists in the food industry and extraction technologies. This review addresses crucial aspects of shikonin deserving of further exploration. It begins by elucidating the attributes of the Boraginaceae plants and their medicinal traits in folklore. It proceeds to focus on the roots of the plant and its medicinal properties, followed by extraction procedures explored in the last fifteen years, emphasising the novel technologies that have been chosen to improve the yield extract while minimising extraction times. Furthermore, this review briefly outlines studies employing cell culture techniques to enhance in vitro shikonin production. Lastly, attention is directed towards research in the food industry, particularly on shikonin-loaded biodegradable films and the antioxidant activity of shikonin. This review concludes by summarising the future potential in food science and prominent research gaps in this field. Full article

Panax notoginseng is a highly regarded medicinal plant that has obstacles associated with continuous cropping. Understanding soil microorganisms is crucial, as they play a major role in this regard. However, soil microorganisms are affected by multiple factors; therefore, we need to conduct more in-depth research. This study investigated the combined effects of irrigation and microbial fertilizer treatments (J1F1, J1F2, J2F1, J2F2, J3F1, J3F2, and CK) on the diversity of bacterial and fungal microbial communities in the rhizosphere of Panax notoginseng. The bacterial 16S rRNA genes and fungal internal transcribed spacer (ITS) sequences were sequenced using Illumina HiSeq. The results showed that, without microbial fertilizer (CK), the microbial community abundance and diversity were significantly lower than in the other treatments; moreover, among the microbial fertilizer treatments, the microbial abundance in F1 was higher than that in F2. Under the same microbial fertilizer application, the incidence rate of Panax notoginseng root rot was J2 > J1 > J3, and the yield of Panax notoginseng was J3 > J2 > J1. Under the same irrigation conditions, the incidence rate of Panax notoginseng root rot was F1 > F2, and the yield of Panax notoginseng was F2 > F1. This study provides important guidance for Panax notoginseng plant microbiota and sustainable agriculture. Full article

Accurate and fast recognition of vehicle license plates from natural scene images is a crucial and challenging task. Existing methods can recognize license plates in simple scenarios, but their performance degrades significantly in complex environments. A novel license plate detection and recognition model YOLOv5-PDLPR is proposed, which employs YOLOv5 target detection algorithm in the license plate detection part and uses the PDLPR algorithm proposed in this paper in the license plate recognition part. The PDLPR algorithm is mainly designed as follows: (1) A Multi-Head Attention mechanism is used to accurately recognize individual characters. (2) A global feature extractor network is designed to improve the completeness of the network for feature extraction. (3) The latest parallel decoder architecture is adopted to improve the inference efficiency. The experimental results show that the proposed algorithm has better accuracy and speed than the comparison algorithms, can achieve real-time recognition, and has high efficiency and robustness in complex scenes. Full article

We study the properties of the constructive linear programming problems. The parameters of linear functions in such problems are constructive real numbers. Solving such a problem involves finding the optimal plan with the constructive real number components. We show that it is impossible to have an algorithm that solves an arbitrary constructive real programming problem. Full article

Gas bearings have the advantages of small friction loss, wide applicable speed range, no pollution, etc., and have important application prospects in micro and small high-speed rotating machinery. However, due to its compressibility and low viscosity, its dynamic stability in high-speed rotating machinery is the key to constraining its development. The experimental study of shaft system dynamics is the main means to explore the mechanism of rotor behavior. On the test platform of dynamic characteristics of multi-disc rotor system supported by aerostatic bearings, experimental research on the nonlinear dynamic characteristics of a rotor system was carried out, and nonlinear vibration test and analysis methods, such as axial orbits, bifurcation diagrams, and spectral characteristics, were adopted, and vibration phenomena, including the critical rotational speed accumulating energy and low-frequency accumulating energy, were presented and the vibration characteristics of bearing fracture faults were presented. The bearing supply pressure and rubber damping pad were introduced as a method to suppress the low-frequency vibration of the aerostatic bearing rotor system, and its vibration-reduction effect was verified by experiments. The above results can provide technical support for vibration control and fault diagnosis of rotor systems supported by aerostatic bearings. Full article