The 2023 MDPI Annual Report has
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13 pages, 1941 KiB  
Article
Melatonin Modulates Lipid Metabolism and Reduces Cardiovascular Risk in Apolipoprotein E-Deficient Mice Fed a Western Diet
by Guillermo Santos-Sánchez, Ana Isabel Álvarez-López, Eduardo Ponce-España, Ana Isabel Álvarez-Ríos, Patricia Judith Lardone, Antonio Carrillo-Vico and Ivan Cruz-Chamorro
Nutraceuticals 2024, 4(2), 260-272; https://doi.org/10.3390/nutraceuticals4020016 - 9 May 2024
Abstract
Melatonin (MLT), a natural compound found in the animal and vegetable kingdom, participates in several physiological processes. MLT exerts antioxidant and anti-inflammatory activities, among others, but information about its action on lipid metabolism is still scarce. For this reason, mice deficient in apolipoprotein [...] Read more.
Melatonin (MLT), a natural compound found in the animal and vegetable kingdom, participates in several physiological processes. MLT exerts antioxidant and anti-inflammatory activities, among others, but information about its action on lipid metabolism is still scarce. For this reason, mice deficient in apolipoprotein E (ApoE−/−) fed a Western diet (WD) were intragastrically treated with different concentrations of MLT (2 and 9 mg/kg) for 12 weeks. The lipid parameters were quantified, and, since links between cardiovascular risk and immune function and oxidative stress have been established, we also analyzed the population of leukocytes and the oxidative stress status. Although there was no change in the weight of the mice, a significant reduction in low-density lipoprotein cholesterol (LDL-C) was observed in mice treated with the higher concentration of MLT tested in this study. Additionally, an improvement in cardiovascular risk indexes was observed. A reduction in the hepatic total cholesterol (TC) and LDL-C levels was also observed in the treated mice. Finally, a decrease in leukocytes and lymphocytes in particular, as well as an increase in the antioxidant status, were shown in MLT-treated mice. In conclusion, MLT is a promising candidate that could be considered as a possible functional ingredient capable of preventing cardiovascular risk. Full article
(This article belongs to the Special Issue Functional Foods as a New Therapeutic Strategy 2.0)
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16 pages, 2878 KiB  
Article
Method for Detecting Pathology of Internal Organs Using Bioelectrography
by Yulia Shichkina, Roza Fatkieva, Alexander Sychev and Anatoliy Kazak
Diagnostics 2024, 14(10), 991; https://doi.org/10.3390/diagnostics14100991 (registering DOI) - 9 May 2024
Abstract
This article considers the possibility of using the bioelectrography method to identify the pathology of internal organs. It is shown that with the currently existing methods, there is no possibility of the automatic detection of diseases or abnormalities in the functioning of a [...] Read more.
This article considers the possibility of using the bioelectrography method to identify the pathology of internal organs. It is shown that with the currently existing methods, there is no possibility of the automatic detection of diseases or abnormalities in the functioning of a particular organ, or of the definition of combined pathology. It has been revealed that the use of various classifiers makes it possible to expand the field of pathology and choose the most optimal method for determining a particular disease. Based on this, a method for detecting the pathology of internal organs is developed, as well as a software package that allows the detection of diseases of the internal organs based on the bioelectrography results. Machine-learning models such as logistic regression, decision tree, random forest, xgboost, KNN, SVM and HyperTab are used for this purpose. HyperTab, logistic regression and xgboost turn out to be the best among them for this task, achieving a performance according to the f1-score metric in the order of 60–70%. The use of the developed method will, in practice, allow us to switch to combining various machine-learning models for the identification of certain diseases, as well as for the identification of combined pathology, which will help solve the problem of detecting pathology during screening studies and lead to a reduction in the burden on the staff of medical institutions. Full article
(This article belongs to the Section Machine Learning and Artificial Intelligence in Diagnostics)
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23 pages, 21242 KiB  
Article
Spatiotemporal Evolution of Land Cover and Landscape Ecological Risk in Wuyishan National Park and Surrounding Areas
by Yuzhi Liu, Wenping Cao and Fuyuan Wang
Land 2024, 13(5), 646; https://doi.org/10.3390/land13050646 (registering DOI) - 9 May 2024
Abstract
Previous studies on national park conservation have focused mainly on their internal areas, and few studies have focused on national parks and their surrounding areas as unified entities. Therefore, the investigation of land cover and landscape ecological risks in national parks and surrounding [...] Read more.
Previous studies on national park conservation have focused mainly on their internal areas, and few studies have focused on national parks and their surrounding areas as unified entities. Therefore, the investigation of land cover and landscape ecological risks in national parks and surrounding areas is essential for overall ecosystem protection and regional sustainable development. This study examines the spatiotemporal evolution of land cover and its landscape ecological risk in Wuyishan National Park, China, and its surrounding areas (WNPSA) from 1990 to 2020. The results show that (1) the land cover of WNPSA from 1990 to 2020 predominantly exhibited a consistent decline in forested areas, paralleled by an augmentation in farmland and impervious surface areas. The center of standard deviation ellipse of impervious surfaces has been progressively moving further south in tandem with the expansion of these surfaces, primarily located within the county town of Fujian Province. (2) The Wuyishan National Park (WNP) areas were dominated by low values of landscape index, and the high value areas in the park were mainly located at the provincial boundary area, with a gradual narrowing during 1990–2020, suggesting a decrease in landscape heterogeneity within the park. High value areas in the surrounding areas mainly occurred in areas with clustered impervious surfaces (e.g., the county town), where part of them located in Wuyishan City have spread to the edge area southeast of WNP. (3) From 1990 to 2020, the lowest-risk areas continue to expand. However, as the medium-risk zone of the adjacent region extends into the edge of the national park, the low risk zone within the national park exhibits a trend from continuous to separate with the neighboring low risk zone. This led to an escalating stress effect on the ecological security of both the adjacent regions and the national park’s boundary areas due to land cover changes. Full article
(This article belongs to the Special Issue Ecological Restoration and Reusing Brownfield Sites)
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18 pages, 1540 KiB  
Article
Hierarchical Vector-Quantized Variational Autoencoder and Vector Credibility Mechanism for High-Quality Image Inpainting
by Cheng Li, Dan Xu and Kuai Chen
Electronics 2024, 13(10), 1852; https://doi.org/10.3390/electronics13101852 (registering DOI) - 9 May 2024
Abstract
Image inpainting infers the missing areas of a corrupted image according to the information of the undamaged part. Many existing image inpainting methods can generate plausible inpainted results from damaged images with the fast-developed deep-learning technology. However, they still suffer from over-smoothed textures [...] Read more.
Image inpainting infers the missing areas of a corrupted image according to the information of the undamaged part. Many existing image inpainting methods can generate plausible inpainted results from damaged images with the fast-developed deep-learning technology. However, they still suffer from over-smoothed textures or textural distortion in the cases of complex textural details or large damaged areas. To restore textures at a fine-grained level, we propose an image inpainting method based on a hierarchical VQ-VAE with a vector credibility mechanism. It first trains the hierarchical VQ-VAE with ground truth images to update two codebooks and to obtain two corresponding vector collections containing information on ground truth images. The two vector collections are fed to a decoder to generate the corresponding high-fidelity outputs. An encoder then is trained with the corresponding damaged image. It generates vector collections approximating the ground truth by the help of the prior knowledge provided by the codebooks. After that, the two vector collections pass through the decoder from the hierarchical VQ-VAE to produce the inpainted results. In addition, we apply a vector credibility mechanism to promote vector collections from damaged images and approximate vector collections from ground truth images. To further improve the inpainting result, we apply a refinement network, which uses residual blocks with different dilation rates to acquire both global information and local textural details. Extensive experiments conducted on several datasets demonstrate that our method outperforms the state-of-the-art ones. Full article
(This article belongs to the Special Issue Applications of Artificial Intelligence in Image and Video Processing)
15 pages, 960 KiB  
Review
Endovascular Stent-Graft Repair of True and False Aneurysms of the Splenic Artery
by Ottavia Borghese, Angelo Pisani, Antonio Luparelli, Simona Sica, Fabrizio Minelli, Tommaso Donati and Yamume Tshomba
J. Clin. Med. 2024, 13(10), 2802; https://doi.org/10.3390/jcm13102802 (registering DOI) - 9 May 2024
Abstract
Objective: In treatment of aneurysms (SAAs) and pseudoaneurysms (SAPs) of the splenic artery, endovascular coil embolization is the approach most commonly used as it is minimally invasive and safe. However, it carries a significant rate of primary failure (up to 30%) and might [...] Read more.
Objective: In treatment of aneurysms (SAAs) and pseudoaneurysms (SAPs) of the splenic artery, endovascular coil embolization is the approach most commonly used as it is minimally invasive and safe. However, it carries a significant rate of primary failure (up to 30%) and might be complicated by splenic infarction. The use of stent grafts might represent a valuable alternative when specific anatomical criteria are respected. We report a comprehensive review on technical and clinical outcomes achieved in this setting. Methods: We performed a comprehensive review of the literature through the MedLine and Cochrane databases (from January 2000 to December 2023) on reported cases of stenting for SAAs and SAPs. Outcomes of interest were clinical and technical success and related complications. The durability of the procedure in the long-term was also investigated. Results: Eighteen papers were included in the analysis, totalling 41 patients (n = 20 male 48.8%, mean age 55.5, range 32–82 years; n = 31, 75.6% SAAs). Mean aneurysm diameter in non-ruptured cases was 35 mm (range 20–67 mm), and most lesions were detected at the proximal third of the splenic artery. Stent grafting was performed in an emergent setting in n = 10 (24.3%) cases, achieving immediate clinical and technical success rate in 90.2% (n = 37) of patients regardless of the type of stent-graft used. There were no procedure-related deaths, but one patient died in-hospital from septic shock and n = 2 (4.9%) patients experienced splenic infarction. At the last available follow-up, the complete exclusion of the aneurysm was confirmed in 87.8% of cases (n = 36/41), while no cases of aneurysm growing nor endoleak were reported. None of the patients required re-intervention during follow-up. Conclusions: When specific anatomical criteria are respected, endovascular repair of SAAs and SAAPs using stent grafts appears to be safe and effective, and seems to display a potential advantage in respect to simple coil embolization, preserving the patient from the risk of end-organ ischemia. Full article
(This article belongs to the Special Issue "Visceral Aneurysm" in 2022: Recent Advances and Treatment)
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17 pages, 806 KiB  
Review
Exosome-like Systems: From Therapies to Vaccination for Cancer Treatment and Prevention—Exploring the State of the Art
by Hamid Heydari Sheikhhossein, Francesca Iommelli, Natalia Di Pietro, Maria Cristina Curia, Adriano Piattelli, Rosanna Palumbo, Giovanni N. Roviello and Viviana De Rosa
Vaccines 2024, 12(5), 519; https://doi.org/10.3390/vaccines12050519 (registering DOI) - 9 May 2024
Abstract
Cancer remains one of the main causes of death in the world due to its increasing incidence and treatment difficulties. Although significant progress has been made in this field, innovative approaches are needed to reduce tumor incidence, progression, and spread. In particular, the [...] Read more.
Cancer remains one of the main causes of death in the world due to its increasing incidence and treatment difficulties. Although significant progress has been made in this field, innovative approaches are needed to reduce tumor incidence, progression, and spread. In particular, the development of cancer vaccines is currently ongoing as both a preventive and therapeutic strategy. This concept is not new, but few vaccines have been approved in oncology. Antigen-based vaccination emerges as a promising strategy, leveraging specific tumor antigens to activate the immune system response. However, challenges persist in finding suitable delivery systems and antigen preparation methods. Exosomes (EXs) are highly heterogeneous bilayer vesicles that carry several molecule types in the extracellular space. The peculiarity is that they may be released from different cells and may be able to induce direct or indirect stimulation of the immune system. In particular, EX-based vaccines may cause an anti-tumor immune attack or produce memory cells recognizing cancer antigens and inhibiting disease development. This review delves into EX composition, biogenesis, and immune-modulating properties, exploring their role as a tool for prevention and therapy in solid tumors. Finally, we describe future research directions to optimize vaccine efficacy and realize the full potential of EX-based cancer immunotherapy. Full article
(This article belongs to the Special Issue Advances in Cancer Immunotherapy and Vaccines Research: 2nd Edition)
18 pages, 2121 KiB  
Article
Molecular Dynamics Simulation on Solidification Microstructure and Tensile Properties of Cu/SiC Composites
by Wanjun Yan, Yuhang Lu, Tinghong Gao, Junjie Wang, Xin Tang and Nan Wang
Molecules 2024, 29(10), 2230; https://doi.org/10.3390/molecules29102230 (registering DOI) - 9 May 2024
Abstract
The shape of ceramic particles is one of the factors affecting the properties of metal matrix composites. Exploring the mechanism of ceramic particles affecting the cooling mechanical behavior and microstructure of composites provides a simulation basis for the design of high-performance composites. In [...] Read more.
The shape of ceramic particles is one of the factors affecting the properties of metal matrix composites. Exploring the mechanism of ceramic particles affecting the cooling mechanical behavior and microstructure of composites provides a simulation basis for the design of high-performance composites. In this study, molecular dynamics methods are used for investigating the microstructure evolution mechanism in Cu/SiC composites containing SiC particles of different shapes during the rapid solidification process and evaluating the mechanical properties after cooling. The results show that the spherical SiC composites demonstrate the highest degree of local ordering after cooling. The more ordered the formation is of face-centered-cubic and hexagonal-close-packed structures, the better the crystallization is of the final composite and the less the number of stacking faults. Finally, the results of uniaxial tensile in three different directions after solidification showed that the composite containing spherical SiC particles demonstrated the best mechanical properties. The findings of this study provide a reference for understanding the preparation of Cu/SiC composites with different shapes of SiC particles as well as their microstructure and mechanical properties and provide a new idea for the experimental and theoretical research of Cu/SiC metal matrix composites. Full article
(This article belongs to the Topic Advances in Computational Materials Sciences)
14 pages, 524 KiB  
Review
Some Recent Advances in Germanium Recovery from Various Resources
by Francisco Jose Alguacil and Jose Ignacio Robla
Metals 2024, 14(5), 559; https://doi.org/10.3390/met14050559 (registering DOI) - 9 May 2024
Abstract
Though nowadays germanium does not reach the range of popularity of other metals, i.e., rare earth elements, its utility in target industries makes it a strategic metal. Though germanium can be found in a series of raw materials, the principal source for its [...] Read more.
Though nowadays germanium does not reach the range of popularity of other metals, i.e., rare earth elements, its utility in target industries makes it a strategic metal. Though germanium can be found in a series of raw materials, the principal source for its recovery is from secondary wastes of the zinc industry; also, the recyclability of germanium-bearing waste materials is becoming of interest. In this recovery and due to the size of the target materials, because the diffusion and reaction are to be considered, hydrometallurgy performs a key role in achieving this goal. The present work reviews the most recent applications (2023 and 2024 years) of hydrometallurgical operations on the recovery of germanium from different solid and liquid sources. Full article
(This article belongs to the Special Issue Advances in Sustainable Hydrometallurgy)
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23 pages, 6506 KiB  
Article
Selection of the Discriming Feature Using the BEMD’s BIMF for Classification of Breast Cancer Mammography Image
by Fatima Ghazi, Aziza Benkuider, Fouad Ayoub and Khalil Ibrahimi
BioMedInformatics 2024, 4(2), 1202-1224; https://doi.org/10.3390/biomedinformatics4020066 (registering DOI) - 9 May 2024
Abstract
Mammogram exam images are useful in identifying diseases, such as breast cancer, which is one of the deadliest cancers, affecting adult women around the world. Computational image analysis and machine learning techniques can help experts identify abnormalities in these images. In this work [...] Read more.
Mammogram exam images are useful in identifying diseases, such as breast cancer, which is one of the deadliest cancers, affecting adult women around the world. Computational image analysis and machine learning techniques can help experts identify abnormalities in these images. In this work we present a new system to help diagnose and analyze breast mammogram images. To do this, the system a method the Selection of the Most Discriminant Attributes of the images preprocessed by BEMD “SMDA-BEMD”, this entails picking the most pertinent traits from the collection of variables that characterize the state under study. A reduction of attribute based on a transformation of the data also called an extraction of characteristics by extracting the Haralick attributes from the Co-occurrence Matrices Methods “GLCM” this reduction which consists of replacing the initial set of data by a new reduced set, constructed at from the initial set of features extracted by images decomposed using Bidimensional Empirical Multimodal Decomposition “BEMD”, for discrimination of breast mammogram images (healthy and pathology) using BEMD. This decomposition makes it possible to decompose an image into several Bidimensional Intrinsic Mode Functions “BIMFs” modes and a residue. The results obtained show that mammographic images can be represented in a relatively short space by selecting the most discriminating features based on a supervised method where they can be differentiated with high reliability between healthy mammographic images and pathologies, However, certain aspects and findings demonstrate how successful the suggested strategy is to detect the tumor. A BEMD technique is used as preprocessing on mammographic images. This suggested methodology makes it possible to obtain consistent results and establishes the discrimination threshold for mammography images (healthy and pathological), the classification rate is improved (98.6%) compared to existing cutting-edge techniques in the field. This approach is tested and validated on mammographic medical images from the Kenitra-Morocco reproductive health reference center (CRSRKM) which contains breast mammographic images of normal and pathological cases. Full article
(This article belongs to the Special Issue Feature Papers on Methods in Biomedical Informatics)
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13 pages, 753 KiB  
Article
Advancing Age May Decrease Mitochondrial Activity in Cumulus Cells
by Suwichaya Jitngamsujarit, Lingling Salang, Charupong Saengboonmee, Supannika Sorin, Kanyarat Thithuan, Thanida Pongsritasana and Sineenart Sukkasame
J. Clin. Med. 2024, 13(10), 2800; https://doi.org/10.3390/jcm13102800 (registering DOI) - 9 May 2024
Abstract
Background: The goal of this study was to compare mitochondrial activity in cumulus cells (CCs) between young and advancing-aged women, the factors that affect mitochondrial activity, and their association with blastocyst quality. Materials and methods: This prospective study included 80 infertile women who [...] Read more.
Background: The goal of this study was to compare mitochondrial activity in cumulus cells (CCs) between young and advancing-aged women, the factors that affect mitochondrial activity, and their association with blastocyst quality. Materials and methods: This prospective study included 80 infertile women who underwent ICSI between May and October 2023. Participants were divided into two groups: older and younger than 38. The oocyte mitochondrial activity from CCs was evaluated using MitoTracker, and the mean fluorescence intensity (MFI) was also evaluated. Results: The univariate and multivariate analyses revealed a significant difference in the MFI between the woman ≥ 38 age group and the lower age group (162.68 ± 79.87 vs. 228.39± 121.38; p-value = 0.005; 95%CI 19.97, 111.45). The factors that affected the MFI were women ≥ 38 years of age (p-value = 0.005; 95%CI −111.45, −19.91), total gonadotropin dosages (p-value = 0.006; 95%CI −0.08, 0.01), and gonadotropin-releasing hormone agonist (GnRHa) triggering (p-value = 0.006; 95%CI 36.46, 210.06). However, only women aged ≥38 years remained statistically significant after a multivariable regression analysis (p-value = 0.014; 95%CI −121.00, −14.30). In addition, only male age (mean age ± SD = 38.26 ± 5.13) was associated with high blastocyst quality in univariate and mixed multivariate analyses (OR 0.91; 95%CI 0.56, 3.04). The chemical pregnancy rate was not significantly different between the two age groups (34.5% vs. 56.7%; p-value = 0.162; 95%CI 0.2, 1.30). Conclusion: Advancing age decreased mitochondrial activity in CCs but did not affect blastocyst quality. By contrast, male age may be a predictor of high-grade blastocyst quality. Full article
23 pages, 3853 KiB  
Review
The Use of Spectroscopic Methods to Study Organic Matter in Virgin and Arable Soils: A Scoping Review
by Evgeny Lodygin and Evgeny Abakumov
Agronomy 2024, 14(5), 1003; https://doi.org/10.3390/agronomy14051003 (registering DOI) - 9 May 2024
Abstract
The use of modern spectroscopic methods of analysis, which provide extensive information on the chemical nature of substances, significantly expands our understanding of the molecular composition and properties of soil organic matter (SOM) and its transformation and stabilization processes in various ecosystems and [...] Read more.
The use of modern spectroscopic methods of analysis, which provide extensive information on the chemical nature of substances, significantly expands our understanding of the molecular composition and properties of soil organic matter (SOM) and its transformation and stabilization processes in various ecosystems and geochemical conditions. The aim of this review is to identify and analyze studies related to the application of nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopy techniques to study the molecular composition and transformation of organic matter in virgin and arable soils. This article is mainly based on three research questions: (1) Which NMR spectroscopy techniques are used to study SOM, and what are their disadvantages and advantages? (2) How is the NMR spectroscopy technique used to study the molecular structure of different pools of SOM? (3) How is ESR spectroscopy used in SOM chemistry, and what are its advantages and limitations? Relevant studies published between 1996 and 2024 were searched in four databases: eLIBRARY, MDPI, ScienceDirect and Springer. We excluded non-English-language articles, review articles, non-peer-reviewed articles and other non-article publications, as well as publications that were not available according to the search protocols. Exclusion criteria for articles were studies that used NMR and EPR techniques to study non-SOM and where these techniques were not the primary methods. Our scoping review found that both solid-state and solution-state NMR spectroscopy are commonly used to study the structure of soil organic matter (SOM). Solution-phase NMR is particularly useful for studying soluble SOM components of a low molecular weight, whereas solid-phase NMR offers advantages such as higher 13C atom concentration for stronger signals and faster analysis time. However, solution-phase NMR has limitations including sample insolubility, potential signal aggregation and reduced sensitivity and resolution. Solid-state NMR is better at detecting non-protonated carbon atoms and identifying heterogeneous regions within structures. EPR spectroscopy, on the other hand, offers significant advantages in experimental biochemistry due to its high sensitivity and ability to provide detailed information about substances containing free radicals (FRs), aiding in the assessment of their reactivity and transformations. Understanding the FR structure in biopolymers can help to study the formation and transformation of SOM. The integration of two- and three-dimensional NMR spectroscopy with other analytical methods, such as chromatography, mass spectrometry, etc., provides a more comprehensive approach to deciphering the complex composition of SOM than one-dimensional techniques alone. Full article
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12 pages, 556 KiB  
Article
Prevalences and Interrelationships of Post COVID-19 Fatigue, Sleep Disturbances, and Depression in Healthy Young and Middle-Aged Adults
by Changhwan Kim, Jae Young Moon, Sung Hyun Kim, Sun-Hyung Kim, Youjin Chang, Woo Hyun Cho, Won-Young Kim, Sun Jung Kwon, Ho Cheol Kim, Kwang Ha Yoo and Young Seok Lee
J. Clin. Med. 2024, 13(10), 2801; https://doi.org/10.3390/jcm13102801 (registering DOI) - 9 May 2024
Abstract
An evaluation of the persistence of symptoms following COVID-19 in economically active young and middle-aged adults is crucial due to its significant socioeconomic impact resulting from compromised work performance. Methods: A prospective, multicenter study at 12 South Korean hospitals from January to December [...] Read more.
An evaluation of the persistence of symptoms following COVID-19 in economically active young and middle-aged adults is crucial due to its significant socioeconomic impact resulting from compromised work performance. Methods: A prospective, multicenter study at 12 South Korean hospitals from January to December 2022 involved telephone interviews along with validated questionnaires. Results: Among 696 participants with a median age of 32 and no prior diagnoses, 30% of participants experienced persistent fatigue, while 21.4% suffered from sleep disturbance at 6 months following infection. Additionally, approximately 25% of the participants exhibited depression that endured for up to 6 months. Symptomatic individuals at 3 months exhibited a significantly higher prevalence of persistent fatigue, sleep disturbances, and depression at 6 months compared to those who remained asymptomatic. Notably, sleep disturbance and persistent fatigue at 3 months emerged as significant independent predictors of the presence of depression at 6 months. Conclusions: Even among young and middle-aged healthy adults, prolonged fatigue, sleep disturbance, and depression exhibit a significant prevalence and persisted for up to 6 months. Therefore, implementing a workplace management protocol for these symptoms is essential to mitigate the socioeconomic burden caused by the impairment of work efficiency. Full article
(This article belongs to the Section Pulmonology)
14 pages, 486 KiB  
Article
Palladium-Catalyzed Arylations towards 3,6-Diaryl-1,3a,6a-triazapentalenes and Evaluation of Their Fluorescence Properties
by Yingchun Wang, Tomas Opsomer, Flip de Jong, Davy Verhaeghe, Maarten Mulier, Luc Van Meervelt, Mark Van der Auweraer and Wim Dehaen
Molecules 2024, 29(10), 2229; https://doi.org/10.3390/molecules29102229 (registering DOI) - 9 May 2024
Abstract
Methyl 4-(1,3a,6a-triazapentalen-3-yl)benzoate (TAP1) shows interesting properties as a small molecule fluorophore. In the search for post-functionalization methods, palladium-catalyzed arylation reactions were demonstrated. Direct CH arylation reactions of TAP1 with various aryl halides resulted in 3,6-diaryltriazapentalenes TAP4, although mostly in poor [...] Read more.
Methyl 4-(1,3a,6a-triazapentalen-3-yl)benzoate (TAP1) shows interesting properties as a small molecule fluorophore. In the search for post-functionalization methods, palladium-catalyzed arylation reactions were demonstrated. Direct CH arylation reactions of TAP1 with various aryl halides resulted in 3,6-diaryltriazapentalenes TAP4, although mostly in poor yields. Bromination of TAP1 followed by Suzuki coupling, on the other hand, requires a more delicate procedure, but gave arylated products with the same regiochemistry (TAP4) in moderate to good yields. The structure of 6-phenyltriazapentalene TAP4a was confirmed by crystallographic analysis. In addition, the effect of the C6 arylation on the fluorescent properties of 3-aryl-1,3a,6a-triazapentalenes was studied in dichloromethane at room temperature and in 2-methyltetrahydrofuran at 77K, while the photophysical properties of two saponified derivatives were measured in acetonitrile. Full article
(This article belongs to the Special Issue Heterocyclic Chemistry in Europe)
14 pages, 3206 KiB  
Article
Research on the Functional Microbe Activation System in a Post-Polymer Flooded Reservoir
by Yinsong Liu, Min Wang, Haiwen Wei, Xiaolin Wu, Zhaowei Hou, Xiumei Zhang and Erlong Yang
Processes 2024, 12(5), 967; https://doi.org/10.3390/pr12050967 (registering DOI) - 9 May 2024
Abstract
Further exploitation of the residual oil underground in post-polymer flooded reservoirs is attractive and challenging. Microbial-enhanced oil recovery (MEOR) is a promising strategy to enhance the recovery of residual oil in post-polymer flooded reservoirs. Identifying and selectively activating indigenous microorganisms with oil displacement [...] Read more.
Further exploitation of the residual oil underground in post-polymer flooded reservoirs is attractive and challenging. Microbial-enhanced oil recovery (MEOR) is a promising strategy to enhance the recovery of residual oil in post-polymer flooded reservoirs. Identifying and selectively activating indigenous microorganisms with oil displacement capabilities is an urgent requirement in the current design of efficient microbial-enhanced oil recovery technologies. This study combines high-throughput sequencing with functional network analysis to identify the core functional microbes within the reservoirs. Concurrently, it devises targeted activation strategies tailored to oligotrophic conditions through an analysis of environmental factor influences. The feasibility of these strategies is then validated through physical simulation experiments. With nutrient stimulation, the overall diversity of microorganisms decreases while the abundance of functional microorganisms increases. The core displacement results showed that the oil recovery factor increased by 3.82% on the basis of polymer flooding. In summary, this research has established a system for the efficient activation of functional microorganisms under oligotrophic conditions by utilizing bioinformatics, network analysis, and indoor simulation systems. This achievement will undoubtedly lay a solid foundation for the practical implementation of microbial enhancement techniques in the field. Full article
(This article belongs to the Section Energy Systems)
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20 pages, 8206 KiB  
Article
Long-Term Maintenance of Viable Human Endometrial Epithelial Cells to Analyze Estrogen and Progestin Effects
by Muhammad Assad Riaz, Franziska Louisa Kary, Alexandra Jensen, Felix Zeppernick, Ivo Meinhold-Heerlein and Lutz Konrad
Cells 2024, 13(10), 811; https://doi.org/10.3390/cells13100811 (registering DOI) - 9 May 2024
Abstract
There are fewer investigations conducted on human primary endometrial epithelial cells (HPEECs) compared to human primary endometrial stromal cells (HPESCs). One of the main reasons is the scarcity of protocols enabling prolonged epithelial cell culture. Even though it is possible to culture HPEECs [...] Read more.
There are fewer investigations conducted on human primary endometrial epithelial cells (HPEECs) compared to human primary endometrial stromal cells (HPESCs). One of the main reasons is the scarcity of protocols enabling prolonged epithelial cell culture. Even though it is possible to culture HPEECs in 3D over a longer period of time, it is technically demanding. In this study, we successfully established a highly pure, stable, and long-term viable human conditionally reprogrammed endometrial epithelial cell line, designated as eCRC560. These cells stained positive for epithelial markers, estrogen and progesterone receptors, and epithelial cell–cell contacts but negative for stromal and endothelial cell markers. Estradiol (ES) reduced the abundance of ZO-1 in a time- and dose-dependent manner, in contrast to the dose-dependent increase with the progestin dienogest (DNG) when co-cultured with HPESCs. Moreover, ES significantly increased cell viability, cell migration, and invasion of the eCRC560 cells; all these effects were inhibited by pretreatment with DNG. DNG withdrawal led to a significantly disrupted monolayer of eCRC560 cells in co-culture with HPESCs, yet it markedly increased the adhesion of eCRC560 to the human mesothelial MeT-5A cells. The long-term viable eCRC560 cells are suitable for in vitro analysis of HPEECs to study the epithelial compartment of the human endometrium and endometrial pathologies. Full article
(This article belongs to the Special Issue Molecular Advances and New Therapeutic Approaches in Endometriosis)
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14 pages, 321 KiB  
Article
Does Quantum Mechanics Require “Conspiracy”?
by Ovidiu Cristinel Stoica
Entropy 2024, 26(5), 411; https://doi.org/10.3390/e26050411 (registering DOI) - 9 May 2024
Abstract
Quantum states containing records of incompatible outcomes of quantum measurements are valid states in the tensor-product Hilbert space. Since they contain false records, they conflict with the Born rule and with our observations. I show that excluding them requires a fine-tuning to an [...] Read more.
Quantum states containing records of incompatible outcomes of quantum measurements are valid states in the tensor-product Hilbert space. Since they contain false records, they conflict with the Born rule and with our observations. I show that excluding them requires a fine-tuning to an extremely restricted subspace of the Hilbert space that seems “conspiratorial”, in the sense that (1) it seems to depend on future events that involve records (including measurement settings) and on the dynamical law (normally thought to be independent of the initial conditions), and (2) it violates Statistical Independence, even when it is valid in the context of Bell’s theorem. To solve the puzzle, I build a model in which, by changing the dynamical law, the same initial conditions can lead to different histories in which the validity of records is relative to the new dynamical law. This relative validity of the records may restore causality, but the initial conditions still must depend, at least partially, on the dynamical law. While violations of Statistical Independence are often seen as non-scientific, they turn out to be needed to ensure the validity of records and our own memories and, by this, of science itself. A Past Hypothesis is needed to ensure the existence of records and turns out to require violations of Statistical Independence. It is not excluded that its explanation, still unknown, ensures such violations in the way needed by local interpretations of quantum mechanics. I suggest that an as-yet unknown law or superselection rule may restrict the full tensor-product Hilbert space to the very special subspace required by the validity of records and the Past Hypothesis. Full article
(This article belongs to the Section Quantum Information)
14 pages, 5725 KiB  
Article
Study on Temperature Cascade ELM Inversion Method for 110 kV Single-Core Cable Intermediate Joints
by Xinhai Li, Bao Feng, Zhengang Wang, Jiangjun Ruan and Chang Xiao
Processes 2024, 12(5), 966; https://doi.org/10.3390/pr12050966 (registering DOI) - 9 May 2024
Abstract
The accurate calculation of the hotspot temperature of the cable intermediate joint can effectively guarantee the safe operation of the transmission and distribution network. This paper addresses the limitations of the current method of estimating hotspot temperature solely from surface temperature measurements. Specifically, [...] Read more.
The accurate calculation of the hotspot temperature of the cable intermediate joint can effectively guarantee the safe operation of the transmission and distribution network. This paper addresses the limitations of the current method of estimating hotspot temperature solely from surface temperature measurements. Specifically, we focus on a 110 kV single-core cable as our subject of study. We started by establishing a simulation model for the temperature field at the intermediate joint to generate data samples. Subsequently, the NCA (neighborhood component analysis) algorithm was employed to select the optimal measurement points on the cable’s surface. This allowed determination of the quantity and location of characteristic points. Finally, we developed a cascading inversion model, which consists of a radial inversion model and an axial inversion model, based on the extreme learning machine algorithm. The example results show that the mean squared error of hotspot temperature obtained by cascade inversion and direct inversion is 6.95 and 24.71, respectively, indicating that cascade inversion can effectively improve the inversion accuracy. Full article
(This article belongs to the Section Energy Systems)
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29 pages, 101396 KiB  
Article
When Two Eyes Don’t Suffice—Learning Difficult Hyperfluorescence Segmentations in Retinal Fundus Autofluorescence Images via Ensemble Learning
by Monty Santarossa, Tebbo Tassilo Beyer, Amelie Bernadette Antonia Scharf, Ayse Tatli, Claus von der Burchard, Jakob Nazarenus, Johann Baptist Roider and Reinhard Koch
J. Imaging 2024, 10(5), 116; https://doi.org/10.3390/jimaging10050116 (registering DOI) - 9 May 2024
Abstract
Hyperfluorescence (HF) and reduced autofluorescence (RA) are important biomarkers in fundus autofluorescence images (FAF) for the assessment of health of the retinal pigment epithelium (RPE), an important indicator of disease progression in geographic atrophy (GA) or central serous chorioretinopathy (CSCR). Autofluorescence images have [...] Read more.
Hyperfluorescence (HF) and reduced autofluorescence (RA) are important biomarkers in fundus autofluorescence images (FAF) for the assessment of health of the retinal pigment epithelium (RPE), an important indicator of disease progression in geographic atrophy (GA) or central serous chorioretinopathy (CSCR). Autofluorescence images have been annotated by human raters, but distinguishing biomarkers (whether signals are increased or decreased) from the normal background proves challenging, with borders being particularly open to interpretation. Consequently, significant variations emerge among different graders, and even within the same grader during repeated annotations. Tests on in-house FAF data show that even highly skilled medical experts, despite previously discussing and settling on precise annotation guidelines, reach a pair-wise agreement measured in a Dice score of no more than 63–80% for HF segmentations and only 14–52% for RA. The data further show that the agreement of our primary annotation expert with herself is a 72% Dice score for HF and 51% for RA. Given these numbers, the task of automated HF and RA segmentation cannot simply be refined to the improvement in a segmentation score. Instead, we propose the use of a segmentation ensemble. Learning from images with a single annotation, the ensemble reaches expert-like performance with an agreement of a 64–81% Dice score for HF and 21–41% for RA with all our experts. In addition, utilizing the mean predictions of the ensemble networks and their variance, we devise ternary segmentations where FAF image areas are labeled either as confident background, confident HF, or potential HF, ensuring that predictions are reliable where they are confident (97% Precision), while detecting all instances of HF (99% Recall) annotated by all experts. Full article
12 pages, 2962 KiB  
Article
Ultrasonic Pretreatment Combined with Microwave-Assisted Hydrodistillation for Extraction of Essential Oil from Melaleuca bracteata ‘Revolution Gold’ Leaves Scales Induced by Cellulase-Inorganic Salt and Its Anti-Fungal Activity
by Yan Huang, Xiaonan Zhang, Fajian Zeng, Jinmei Chang and Zhiwei Liu
Separations 2024, 11(5), 147; https://doi.org/10.3390/separations11050147 (registering DOI) - 9 May 2024
Abstract
In order to further develop the commercial use of Melaleuca bracteata (F. Muell), this report studied the extraction of essential oil from Melaleuca bracteata (F. Muell) leaves using ultrasonic pretreatment, cellulase-inorganic salt soaked and combined with microwave-assisted hydrodistillation. To optimize the primary contributing [...] Read more.
In order to further develop the commercial use of Melaleuca bracteata (F. Muell), this report studied the extraction of essential oil from Melaleuca bracteata (F. Muell) leaves using ultrasonic pretreatment, cellulase-inorganic salt soaked and combined with microwave-assisted hydrodistillation. To optimize the primary contributing parameters, the Box–Behnken design (BBD) was applied. The optimum yield of essential oil was 9.61 mL/kg DW at a microwave power of 510.77 W, lithium chloride dose of 63.56 μmol, and microwave irradiation period of 46.97 min. The essential oil included a total of 41 compounds, and methyl eugenol (76.53%) and methyl cinnamate (12.62%) were the main compounds. The inhibitory impact was notable when the essential oil concentration was 1.6 mg/mL. Therefore, it has the potential to replace chemical pesticides. When the concentration of the essential oil solution was 1.6 mg/mL, the three pathogenic species of fungus (Pseudocercospora psidii, Colletotrichum eriobotryae, and Colletotrichum siamense) were greatly affected; at this dose, the fungus was unable to develop and its growth diameter was 0 mm. Additionally, the fungus’s inhibition rate reached 100%. Full article
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19 pages, 3045 KiB  
Article
Effect of Metformin on Sertoli Cell Fatty Acid Metabolism and Blood–Testis Barrier Formation
by Gustavo Marcelo Rindone, Marina Ercilia Dasso, Cecilia Lucia Centola, Cristian Marcelo Sobarzo, María Noel Galardo, Silvina Beatriz Meroni and María Fernanda Riera
Biology 2024, 13(5), 330; https://doi.org/10.3390/biology13050330 (registering DOI) - 9 May 2024
Abstract
Sertoli cells (SCs) are essential to maintaining germ cell development. Metformin, the main pharmacologic treatment for pediatric type 2 diabetes, is administered to children during SC maturation. The present study aimed to analyze whether metformin affects SC energy metabolism and blood–testis barrier (BTB) [...] Read more.
Sertoli cells (SCs) are essential to maintaining germ cell development. Metformin, the main pharmacologic treatment for pediatric type 2 diabetes, is administered to children during SC maturation. The present study aimed to analyze whether metformin affects SC energy metabolism and blood–testis barrier (BTB) integrity. Primary SC cultures were used for the in vitro studies. In vivo effects were studied in Sprague–Dawley rats treated with 200 mg/kg metformin from Pnd14 to Pnd30. Metformin decreased fatty acid oxidation and increased 3-hydroxybutyrate production in vitro. Moreover, it decreased the transepithelial electrical resistance across the monolayer and induced ZO-1 redistribution, suggesting an alteration of cell junctions. In vivo, a mild but significant increase in BTB permeability and ZO-1 expression was observed in the metformin group, without changes in testicular histology and meiosis progression. Additionally, adult rats that received metformin treatment during the juvenile period showed no alteration in BTB permeability or daily sperm production. In conclusion, metformin exposure may affect BTB permeability in juvenile rats, but this seems not to influence spermatogenesis progression. Considering the results obtained in adult animals, it is possible to speculate that metformin treatment during the juvenile period does not affect testicular function in adulthood. Full article
(This article belongs to the Special Issue Germ, Sertoli and Leydig Cells in the Testis)
22 pages, 1294 KiB  
Article
Coexisting in the Surf Zone: Age and Feeding Habits of the Spotted Seabass (Dicentrarchus punctatus) and European Seabass (Dicentrarchus labrax) on the Gulf of Cádiz Beaches (Southwest Iberian Peninsula)
by Carlos Rodríguez-García, Ana Toro-Podadera, Jesica Sarmiento-Carbajal and Remedios Cabrera-Castro
Fishes 2024, 9(5), 173; https://doi.org/10.3390/fishes9050173 (registering DOI) - 9 May 2024
Abstract
Various commercial fish species utilize different coastal habitats as nurseries in their juvenile stages, with surf zones being particularly crucial due to the protection and abundant food resources they offer. Among the species that rely on these areas are the spotted seabass ( [...] Read more.
Various commercial fish species utilize different coastal habitats as nurseries in their juvenile stages, with surf zones being particularly crucial due to the protection and abundant food resources they offer. Among the species that rely on these areas are the spotted seabass (Dicentrarchus punctatus) and the European seabass (Dicentrarchus labrax). This study aimed to investigate the age and feeding habits of surf zone specimens of these species and explore their environmental adaptations. The average size for D. punctatus was 16.94 ± 4.05 cm, and for D. labrax, it was 23.23 ± 6.30 cm. The length–weight relationship for D. punctatus was TW = 0.013*TL2.885 (parameter a: 95% CI: 0.012–0.014; parameter b: 95% CI: 2.854–2.917), and for D. labrax, it was TW = 0.008*TL3.095 (parameter a: 95% CI: 0.006–0.009; parameter b: 95% CI: 3.040–3.151). In both D. punctatus (79.20%) and D. labrax (75.92%), the predominant age classes were 1+ and 2+. Significant variations in age class abundance were observed in D. punctatus based on the time of day, lunar phase, and season, while D. labrax showed variations only by season. A diet analysis revealed that both species primarily consume mysids and fish, with D. punctatus showing dietary variations related to the time of day, lunar phase, and season, and D. labrax showing variations with the time of day and season. Full article
(This article belongs to the Section Biology and Ecology)
20 pages, 7002 KiB  
Article
Optimizing Hadoop Scheduling in Single-Board-Computer-Based Heterogeneous Clusters
by Basit Qureshi
Computation 2024, 12(5), 96; https://doi.org/10.3390/computation12050096 (registering DOI) - 9 May 2024
Abstract
Single-board computers (SBCs) are emerging as an efficient and economical solution for fog and edge computing, providing localized big data processing with lower energy consumption. Newer and faster SBCs deliver improved performance while still maintaining a compact form factor and cost-effectiveness. In recent [...] Read more.
Single-board computers (SBCs) are emerging as an efficient and economical solution for fog and edge computing, providing localized big data processing with lower energy consumption. Newer and faster SBCs deliver improved performance while still maintaining a compact form factor and cost-effectiveness. In recent times, researchers have addressed scheduling issues in Hadoop-based SBC clusters. Despite their potential, traditional Hadoop configurations struggle to optimize performance in heterogeneous SBC clusters due to disparities in computing resources. Consequently, we propose modifications to the scheduling mechanism to address these challenges. In this paper, we leverage the use of node labels introduced in Hadoop 3+ and define a Frugality Index that categorizes and labels SBC nodes based on their physical capabilities, such as CPU, memory, disk space, etc. Next, an adaptive configuration policy modifies the native fair scheduling policy by dynamically adjusting resource allocation in response to workload and cluster conditions. Furthermore, the proposed frugal configuration policy considers prioritizing the reduced tasks based on the Frugality Index to maximize parallelism. To evaluate our proposal, we construct a 13-node SBC cluster and conduct empirical evaluation using the Hadoop CPU and IO intensive microbenchmarks. The results demonstrate significant performance improvements compared to native Hadoop FIFO and capacity schedulers, with execution times 56% and 22% faster than the best_cap and best_fifo scenarios. Our findings underscore the effectiveness of our approach in managing the heterogeneous nature of SBC clusters and optimizing performance across various hardware configurations. Full article
(This article belongs to the Section Computational Engineering)
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14 pages, 3177 KiB  
Article
Comparative Study on the Determination of Chlorophyll-a in Lake Phytoplankton by a YSI Multi-Parameter Water Quality Meter and Laboratory Spectrophotometric Method
by Jie Wang, Lizeng Duan, Donglin Li, Yuwei Zhang, Zheng Yuan, Huayu Li and Hucai Zhang
Water 2024, 16(10), 1350; https://doi.org/10.3390/w16101350 (registering DOI) - 9 May 2024
Abstract
Algal blooms caused by eutrophication are a major global problem, and the monitoring and prediction of algal densities in lakes are important indicators of eutrophication management. However, the reliability of the commonly used chlorophyll-a (Chl-a) to characterize phytoplankton density in [...] Read more.
Algal blooms caused by eutrophication are a major global problem, and the monitoring and prediction of algal densities in lakes are important indicators of eutrophication management. However, the reliability of the commonly used chlorophyll-a (Chl-a) to characterize phytoplankton density in lake environments needs to be further investigated. In this paper, we sampled and analyzed 365 samples from nine plateau lakes in Yunnan Province during the dry and rainy seasons. The Chl-a data measured by the laboratory spectrophotometric method and the portable YSI multi-parameter water quality meter (YSI) directly used in the field were compared, and regression analysis and correlation analysis with phytoplankton density were performed. Most of the Chl-a values measured by the laboratory instrument were greater than those measured by the YSI, and the correlation between the two methods was weak (0.492, p < 0.001). The correlation between Chl-a and phytoplankton density measured by the YSI reached 0.67 (p < 0.001) in the dry season, while the laboratory methods used to measure Chl-a to characterize phytoplankton density may have led to an overestimation of phytoplankton density due to nonspecific sources of Chl-a. However, both methods are relatively inaccurate for characterizing phytoplankton density. For different trophic states of lakes, nutrient concentration changes affect the Chl-a concentration of phytoplankton. During different seasons, changes in the fluorescence intensity of phytoplankton in response to environmental conditions prevent the YSI results from reflecting the authentic phytoplankton density. Furthermore, high species diversity can lead to inconsistent changes in Chl-a and phytoplankton because the content of Chl-a in individual cells of different phytoplankton is different. The relationship between Chl-a and phytoplankton density was species specific. Therefore, when applying Chl-a to characterize phytoplankton density in lakes, it is necessary to consider environmental conditions, phytoplankton community structure and other practical conditions. In addition, laboratory analytical methods and instrumental techniques and instruments need to be improved. Full article

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