The 2023 MDPI Annual Report has
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13 pages, 3292 KiB  
Article
Long-Term Epigenetic Regulation of Foxo3 Expression in Neonatal Valproate-Exposed Rat Hippocampus with Sex-Related Differences
by Eun-Hye Jang and Soon-Ae Kim
Int. J. Mol. Sci. 2024, 25(10), 5287; https://doi.org/10.3390/ijms25105287 (registering DOI) - 13 May 2024
Abstract
Perinatal exposure to valproic acid is commonly used for autism spectrum disorder (ASD) animal model development. The inhibition of histone deacetylases by VPA has been proposed to induce epigenetic changes during neurodevelopment, but the specific alterations in genetic expression underlying ASD-like behavioral changes [...] Read more.
Perinatal exposure to valproic acid is commonly used for autism spectrum disorder (ASD) animal model development. The inhibition of histone deacetylases by VPA has been proposed to induce epigenetic changes during neurodevelopment, but the specific alterations in genetic expression underlying ASD-like behavioral changes remain unclear. We used qPCR-based gene expression and epigenetics tools and Western blotting in the hippocampi of neonatal valproic acid-exposed animals at 4 weeks of age and conducted the social interaction test to detect behavioral changes. Significant alterations in gene expression were observed in males, particularly concerning mRNA expression of Foxo3, which was significantly associated with behavioral changes. Moreover, notable differences were observed in H3K27ac chromatin immunoprecipitation, quantitative PCR (ChIP-qPCR), and methylation-sensitive restriction enzyme-based qPCR targeting the Foxo3 gene promoter region. These findings provide evidence that epigenetically regulated hippocampal Foxo3 expression may influence social interaction-related behavioral changes. Furthermore, identifying sex-specific gene expression and epigenetic changes in this model may elucidate the sex disparity observed in autism spectrum disorder prevalence. Full article
(This article belongs to the Special Issue Molecular Research in Neurodevelopmental Disorders)
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13 pages, 7623 KiB  
Article
Dynamic Marine Atmospheric Corrosion Behavior of AZ91 Mg Alloy Sailing from Yellow Sea to Western Pacific Ocean
by Lihui Yang, Cong Liu, Ying Wang, Xiutong Wang and Haiping Gao
Materials 2024, 17(10), 2294; https://doi.org/10.3390/ma17102294 (registering DOI) - 13 May 2024
Abstract
In this work, the dynamic marine atmospheric corrosion behavior of AZ91 Mg alloy sailing from Yellow Sea to Western Pacific Ocean was studied. The corrosion rates were measured using the weight loss method. The microstructure, phase, and chemical composition of corroded samples were [...] Read more.
In this work, the dynamic marine atmospheric corrosion behavior of AZ91 Mg alloy sailing from Yellow Sea to Western Pacific Ocean was studied. The corrosion rates were measured using the weight loss method. The microstructure, phase, and chemical composition of corroded samples were investigated by SEM, EDS, XRD, and XPS. The results show that the evolution of corrosion rates of AZ91 Mg alloy was divided into three stages: rapidly increasing during the first 3 months, then remaining stable for the next three months, and finally decreasing after 6 months. The annual corrosion rate of Mg alloy reached 32.50 μm/y after exposure for 12 months in a dynamic marine atmospheric environment, which was several times higher than that of the static field exposure tests. AZ91 magnesium alloy was mainly subjected to localized corrosion with more destructiveness to Mg parts, which is mainly due to the synergistic effect of high relative humidity, the high deposition rate of chloride ion, sulfur dioxide acidic gas produced by fuel combustion, and rapid temperature changes caused by the alternating changes in longitude and latitude during navigation. As the exposure time increased, the corrosion pits gradually increased and deepened. The maximum depth of the corrosion pit was 197 μm after 12 months of exposure, which is almost 6 times the average corrosion depth. This study provides scientific data support for the application of magnesium alloys in shipborne aircraft and electronic equipment. The results could provide guidance for the design of new magnesium alloys and development of anti-corrosion technologies. Full article
(This article belongs to the Special Issue Corrosion and Mechanical Behavior of Metal Materials (2nd Edition))
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17 pages, 5838 KiB  
Article
Evaluation and Influential Factors of Urban Land Use Efficiency in Yangtze River Economic Belt
by Dongqing Han and Zhengxu Cao
Land 2024, 13(5), 671; https://doi.org/10.3390/land13050671 (registering DOI) - 13 May 2024
Abstract
The study of urban land use efficiency is of great significance for optimizing the spatial allocation of urban land, thereby promoting the intensive use of urban land and the transformation of economic development modes. Taking the Yangtze River Economic Belt (YREB) as the [...] Read more.
The study of urban land use efficiency is of great significance for optimizing the spatial allocation of urban land, thereby promoting the intensive use of urban land and the transformation of economic development modes. Taking the Yangtze River Economic Belt (YREB) as the study object, we chose the undesirable Slacks-Based Measure (SBM) model to calculate the urban land use efficiency (ULUE). Then, we utilized the spatial correlation analysis and econometric methods to discuss its spatio-temporal features and influential factors. The results show the following: (1) The urban land use efficiency in the YREB steadily improved from 2010 to 2022, but the inter-regional efficiency gap evidently increased. (2) There is an efficiency value to be found in a multi-center network structure, and it forms a “core-periphery” distribution pattern. The high-efficiency areas in the downstream and upstream regions of the YREB are gradually increasing, while the efficiency value in the midstream area remains low. (3) The urban efficiency values have strong correlation, and they are mainly “High-High agglomeration” and “Low-Low agglomeration”, and they show significant regional characteristics. (4) The economic level, industrial structure, and urbanization have obvious motivating effects on ULUE, and the positive spatial spillover effect is clear. The foreign direct investment and land finance hinder the boost of efficiency, and the latter has a negative spatial spillover role on the ULUE in the downstream cities. Full article
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9 pages, 678 KiB  
Article
Defining Trimester-Specific Reference Intervals for Thyroid Hormones: Insights from a Bulgarian Monocenter Study
by Vesselina Yanachkova, Radiana Staynova and Emilia Krassimirova Naseva
Medicina 2024, 60(5), 801; https://doi.org/10.3390/medicina60050801 (registering DOI) - 13 May 2024
Abstract
Background and Objectives: Pregnancy introduces various interfering factors that, alongside individual variations, impact the assessment of thyroid function tests. This underscores the necessity of defining trimester-specific reference intervals for thyroid-stimulating hormone (TSH) levels. Differences in population characteristics, including ethnicity, socio-economic factors, iodine [...] Read more.
Background and Objectives: Pregnancy introduces various interfering factors that, alongside individual variations, impact the assessment of thyroid function tests. This underscores the necessity of defining trimester-specific reference intervals for thyroid-stimulating hormone (TSH) levels. Differences in population characteristics, including ethnicity, socio-economic factors, iodine prophylaxis, and obesity, emphasize the need to establish trimester-specific TSH ranges for women of reproductive age in the respective region or center. The aim of the present study was to establish first- and second-trimester-specific reference intervals for TSH and free thyroxine (FT4) in a relevant pregnant population. Materials and Methods: A retrospective monocenter analysis utilized the electronic database of Ob/Gyn Hospital “Dr. Shterev”, Sofia, Bulgaria. The analysis involved data from 497 pregnant and 250 non-pregnant women, all without evidence of thyroid dysfunction or a family history thereof, no indication of taking medication interfering with thyroid function, no evidence of levothyroxine treatment, and no history of sterility treatment. To establish the limits of the TSH reference range, the percentile method was applied using a bootstrapping procedure following the recommendations of the International Federation of Clinical Chemistry (IFCC). Results: Trimester-specific reference intervals for TSH and FT4 in our center were established as follows: first trimester—0.38–2.91 mU/L, FT4-12.18–19.48 pmol/L; second trimester—0.72–4.22 mIU/L and 9.64–17.39 pmol/L, respectively. We also established the normal reference range for the non-pregnant control group, which is similar to that applicable in our laboratory. Conclusions: Our results differ from the fixed limits recommended by the American Thyroid Association, European Thyroid Association, and Endocrine Society Guidelines. Following the relevant established intervals would significantly impact timely diagnosis and therapy requirements for a substantial proportion of pregnant women. Full article
(This article belongs to the Section Obstetrics and Gynecology)
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19 pages, 3371 KiB  
Article
Oncolytic Tanapoxvirus Recombinants Expressing Flagellin C or Mouse Interleukin-2 Are Capable of Regressing Human Triple-Negative Breast Cancer Xenografts in Immuno-Competent BALB/c Nude Mice
by Michael L. Monaco, Grace A. Filpi, Steven L. Kohler, Robert Eversole, Omer A. Idris and Karim Essani
Pathogens 2024, 13(5), 402; https://doi.org/10.3390/pathogens13050402 (registering DOI) - 13 May 2024
Abstract
Triple-negative breast cancer (TNBC) in humans is the most aggressive and deadly form of BC. Although TNBCs are about 15 percent of the total number of BC cases, they are associated with the highest mortalities. Current treatment options are limited, and most modalities [...] Read more.
Triple-negative breast cancer (TNBC) in humans is the most aggressive and deadly form of BC. Although TNBCs are about 15 percent of the total number of BC cases, they are associated with the highest mortalities. Current treatment options are limited, and most modalities are toxic and have not increased the 5-year survival rates of TNBC. Many oncolytic viruses are emerging as potential therapies for TNBC. In this study, two Tanapoxvirus (TPV) recombinants, one expressing FliC and the other expressing mouse interleukin-2 (mIL-2), were assessed for their efficacy in an immuno-competent xenograft mouse model. MDA-MB-231 tumors were planted in BALB/c nude mice, treated, made immuno-competent via adoptive transfer of splenocytes from healthy BALB/c donors, and then monitored for 40 days. TPV/Δ2L/66R/FliC and TPV/Δ66R/mIL-2 demonstrated significant tumor reduction (p = 0.01602 and p = 0.03890, respectively) compared to the reconstituted control (RC), whereas wtTPV did not. Pathological analyses of treated tumors revealed cells consistent with lymphocyte and plasma cell morphology in reconstituted mice treated with TPV recombinants. Anti-viral plaque reduction assays conducted using harvested serum from treated animals indicated the presence of anti-TPV antibodies in mice reconstituted and treated with TPV that were missing from immune-deficient nude mice, including those exposed to TPV and of statistically equivalent serum concentrations to normal BALB/c mice immunized against TPV. The results suggest immuno-deficient BALB/c nude mice can become immuno-competent via adoptive transfer of splenocytes from genetically identical donors and allow for testing of tumor xenografts in a competent model system. The TPV recombinants tested should be further studied for the potential treatment of human TNBC. Full article
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19 pages, 2614 KiB  
Article
Kinetic Modeling of Co-Pyrogasification in Municipal Solid Waste (MSW) Management: Towards Sustainable Resource Recovery and Energy Generation
by Anabel Fernandez, Daniela Zalazar-García, Carla Lorenzo-Doncel, Diego Mauricio Yepes Maya, Electo Eduardo Silva Lora, Rosa Rodriguez and Germán Mazza
Sustainability 2024, 16(10), 4056; https://doi.org/10.3390/su16104056 (registering DOI) - 13 May 2024
Abstract
This study addresses the co-pyrogasification of municipal solid waste (MSW) from the Environmental Technology Park, San Juan, Argentina. This process involves heating waste at high temperatures in a low-oxygen or oxygen-free atmosphere as a sustainable strategy for waste management and energy generation. The [...] Read more.
This study addresses the co-pyrogasification of municipal solid waste (MSW) from the Environmental Technology Park, San Juan, Argentina. This process involves heating waste at high temperatures in a low-oxygen or oxygen-free atmosphere as a sustainable strategy for waste management and energy generation. The principal objective is to focus on understanding the MSW co-pyrogasification kinetics to enhance performance in reactor design. A representative sample of MSW collected over a month was analyzed, focusing on the variation in mass proportions of plastic, organic matter, and paper. The empirical methodology included the deconvolution of macro-TGA curves and deep learning algorithms to predict and validate macro-TG data during co-pyrogasification. The findings reveal that MSW is a solid matrix more easily treated on thermochemical platforms, with kinetic and thermodynamic parameters favoring its processing. This approach suggests that MSW co-pyrogasification may represent a feasible alternative for resource recovery and bioenergy production, supporting the policies for the transition to a cleaner future and a circular economy. Full article
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11 pages, 212 KiB  
Article
Prison Chaplaincy as A Microaggressive Environment for the Non-Religious
by Katie Hunt
Religions 2024, 15(5), 597; https://doi.org/10.3390/rel15050597 (registering DOI) - 13 May 2024
Abstract
This article unites the Special Issue’s themes of religion, prison, and spaces to examine the prison chaplaincy as a microaggressive environment for people of minority and especially non-religious belief. Although the chaplaincy purports to cater to all faiths and none, it is an [...] Read more.
This article unites the Special Issue’s themes of religion, prison, and spaces to examine the prison chaplaincy as a microaggressive environment for people of minority and especially non-religious belief. Although the chaplaincy purports to cater to all faiths and none, it is an inherently religious institution dominated by the Church of England, whose power and privilege is reinforced in both prison policy and legislation. After setting out the context and methodology of my empirical study, I unpack the concept of microaggressions and share original data from interviews with non-religious chaplaincy volunteers to demonstrate the ways in which prison chaplaincy can be alienating to people with a secular world view both as a pastoral service and a workplace. I also explore the physical space of the chaplaincy as a site of everyday othering, through its layout, language, and imagery. Ultimately, I argue that this facility is not suitable for everyone and creates hierarchies of access in which some prisoners and, indeed, staff feel more welcome than others. The article therefore proposes changes to people, place, and policy that could reduce this microaggressive impact. Full article
21 pages, 2743 KiB  
Article
Oilseed Radish: Nitrogen and Sulfur Management Strategies for Seed Yield and Quality—A Case Study in Poland
by Artur Szatkowski, Zofia Antoszkiewicz, Cezary Purwin and Krzysztof Józef Jankowski
Agriculture 2024, 14(5), 755; https://doi.org/10.3390/agriculture14050755 (registering DOI) - 13 May 2024
Abstract
Nitrogen (N) and sulfur (S) fertilization significantly affect seed yield and quality in Brassica oilseed crops. The effect of N and S management on the crop parameters (plant height, stem-base diameter, and number of branches), yield (seed yield components, seed and straw yields, [...] Read more.
Nitrogen (N) and sulfur (S) fertilization significantly affect seed yield and quality in Brassica oilseed crops. The effect of N and S management on the crop parameters (plant height, stem-base diameter, and number of branches), yield (seed yield components, seed and straw yields, harvest index—HI), and the quality of the seeds and oil (crude fat—CF, total protein—TP, crude fiber—CFR, fatty acids profile—FA, acid detergent fiber; and neutral detergent fiber) of oilseed radish (Raphanus sativus L. var. oleiformis Pers.) was analyzed in the study. The effect of N and S fertilization was evaluated in a field experiment in Bałcyny (north-eastern Poland) in 2020–2022. The experiment had a split-plot design with two factors and three replications. The first factor was the N rate (0, 30, 60, 90, 120 kg ha−1) and the second factor was the S rate (0, 15, 30 kg ha−1). Nitrogen fertilization stimulated stem elongation and branching. The average oilseed radish (OSR) seed yield ranged from 0.59 to 1.15–1.25 Mg ha−1. Seed yields increased significantly, up to 90 kg N ha−1 and 15 kg S ha−1. The N fertilizer use efficiency (NFUE) of OSR decreased with a rise in the N rate (from 4.22 to 2.19 kg of seeds per 1 kg N). The application of S did not increase NFUE. The HI ranged from 10% (0–30 kg N ha−1) to 12% (60 kg N ha−1). The contents of CF, TP, and CFR in OSR seeds (kg−1 dry matter—DM) were 383–384 g, 244–249 g, and 97–103 g, respectively. Nitrogen fertilization decreased the CF content (by 5%) and increased the contents of TP (by 5%) and CFR (by 16%) in OSR seeds. Sulfur fertilizer applied at 30 kg ha−1 decreased the CF content (by 2%), but it did not alter the content of TP or CFR. Oilseed radish oil contained 68–70% of monounsaturated FAs (MUFAs) (erucic acid accounted for 2/3 of the total MUFAs), 24–25% of polyunsaturated FAs (PUFAs), and 6–8% of saturated FAs (SFAs). Nitrogen fertilization increased the proportions of SFAs and PUFAs in OSR oil. Nitrogen rates of 60–90 kg ha−1 increased the contents of alpha-tocopherol (α-T), beta-tocopherol (β-T), and gamma-tocopherol (γ-T) in OSR seeds by 32%, 40%, and 27%, respectively. Sulfur fertilization increased the content of PUFAs and decreased the content of MUFAs in OSR oil, while it increased the contents of α-T (by 15%) and γ-T (by 19%) in OSR seeds. Proper N and S management in OSR cultivation can improve crop productivity and the processing suitability of seeds. Full article
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15 pages, 1853 KiB  
Article
Analytical Study of Nonlinear Flexural Vibration of a Beam with Geometric, Material and Combined Nonlinearities
by Yoganandh Madhuranthakam and Sunil Kishore Chakrapani
Vibration 2024, 7(2), 464-478; https://doi.org/10.3390/vibration7020025 (registering DOI) - 13 May 2024
Abstract
This article explores the nonlinear vibration of beams with different types of nonlinearities. The beam vibration was modeled using Hamilton’s principle, and the equation of motion was solved using method of multiple time scales. Three models were developed assuming (a) geometric nonlinearity, (b) [...] Read more.
This article explores the nonlinear vibration of beams with different types of nonlinearities. The beam vibration was modeled using Hamilton’s principle, and the equation of motion was solved using method of multiple time scales. Three models were developed assuming (a) geometric nonlinearity, (b) material nonlinearity and (c) combined geometric and material nonlinearity. The material nonlinearity also included both third and fourth nonlinear elasticity terms. The frequency response equation of these models were further evaluated quantitatively and qualitatively. The models capture the hardening effect, i.e., increase in resonant frequency as a function of forcing amplitude for geometric nonlinearity, and the softening effect, i.e., decrease in resonant frequency for material nonlinearity. The model is applied on the first three bending modes of the cantilever beam. The effect of the fourth-order material nonlinearity was smaller compared to the third-order term in the first mode, whereas it is significantly larger in second and third mode. The combined nonlinearity models shows a discontinuous frequency shift, which was resolved by utilizing a set of transition assumptions. This results in a smooth transition between the material and geometric zones in amplitude. These parametric models allow us to fine tune the nonlinear response of the system by changing the physical properties such as geometry, linear and nonlinear elastic properties. Full article
(This article belongs to the Special Issue Feature Papers in Vibration)
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14 pages, 1859 KiB  
Article
Breast Cancer Prevention by Dietary Polyphenols: Microemulsion Formulation and In Vitro Studies
by Ghea Putri Cristy, Desy Liana, Jaruwan Chatwichien, Chanat Aonbangkhen, Chantragan Srisomsap and Anuchit Phanumartwiwath
Sci. Pharm. 2024, 92(2), 25; https://doi.org/10.3390/scipharm92020025 (registering DOI) - 13 May 2024
Abstract
Concerns surrounding breast cancer have been increasing, as it leads to the current global cancer incidence and causes a high mortality rate in women. This study investigated the physiological effects of common dietary polyphenols that might prevent breast cancer progression. Quercetin, kaempferol, and [...] Read more.
Concerns surrounding breast cancer have been increasing, as it leads to the current global cancer incidence and causes a high mortality rate in women. This study investigated the physiological effects of common dietary polyphenols that might prevent breast cancer progression. Quercetin, kaempferol, and rosmarinic acid were selected to explore their potential bioactivities. Each polyphenol was formulated into a microemulsion to improve its bioactivity and bioavailability. In vitro antioxidant and cytotoxicity activities of the selected polyphenols and their microemulsion forms were further investigated. The optimized microemulsion carrier with 1% oleic acid, 3% ethanol, 10% polysorbate 20, and 86% ultrapure water achieved more than 90% polyphenol encapsulation efficiency. The microemulsion was stable for more than 30 days when encapsulating polyphenol in the fluctuating temperature treatment. In vitro studies suggested that rosmarinic acid-loaded microemulsion had the best antioxidant activity compared with other polyphenol-loaded microemulsions (PL-MEs). Blank microemulsion and all PL-MEs significantly inhibited the proliferation of both hormone-dependent (T47D) and hormone-independent (MDA-MB-231) breast cancer cells. More studies are warranted to confirm the contribution of the microemulsion carrier components to the polyphenols’ improved antioxidant activity and high toxicity of PL-MEs on breast cancer cells. Full article
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13 pages, 8880 KiB  
Article
Exploring the Origin of Lissajous Geometric Modes from the Ray Tracing Model
by Xin-Liang Zheng, Yu-Han Fang, Wei-Che Chung, Cheng-Li Hsieh and Yung-Fu Chen
Photonics 2024, 11(5), 456; https://doi.org/10.3390/photonics11050456 (registering DOI) - 13 May 2024
Abstract
In this paper, we use the geometric optics and discuss the path of laser beam in a simple laser (concave-plano) cavity with the birefringence crystal. In specific lengths of the laser cavity, we can observe various types of Lissajous-like structural laser modes that [...] Read more.
In this paper, we use the geometric optics and discuss the path of laser beam in a simple laser (concave-plano) cavity with the birefringence crystal. In specific lengths of the laser cavity, we can observe various types of Lissajous-like structural laser modes that can be simulated using our ray tracing model. At the end of this paper, we provide an adjusted ABCD matrix. With the adjusted ABCD matrix and iterative calculation, we can obtain the 3D trajectories which are similar to the experimental results. These structural laser modes can be realized by a Nd:YVO4 solid-state laser with off-axis pumping. From the comparison between the experimental data and the numerical data, we clarify the relationship between the 3D Lissajous-like structural laser modes and ray trajectory in the laser cavity. Full article
(This article belongs to the Special Issue Emerging Topics in Structured Light)
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13 pages, 277 KiB  
Article
Examining the Relationship between Emotional Intelligence, Achievement Motivation, and Self-Efficacy among Pre-Service Teachers in Türkiye
by Umran Atik, Mehmet Akif Karaman and Halil Ibrahim Sari
Educ. Sci. 2024, 14(5), 526; https://doi.org/10.3390/educsci14050526 (registering DOI) - 13 May 2024
Abstract
The main goal of this study was to examine the extent to which the demographic characteristics of pre-service teachers (gender, family management style, family educational status, size of the family) predict their emotional intelligence (EI) scores through achievement motivation (AMO) and Self-Efficacy (SE) [...] Read more.
The main goal of this study was to examine the extent to which the demographic characteristics of pre-service teachers (gender, family management style, family educational status, size of the family) predict their emotional intelligence (EI) scores through achievement motivation (AMO) and Self-Efficacy (SE) scores. The study consisted of 326 pre-service teachers (270 females, 56 males) studying in various departments at faculties of education across three universities located in the Eastern and Southeastern Anatolia regions of Turkiye. The findings indicate that the demographic variables (gender, family management style, family size, family educational status) do not significantly explain the EI levels of pre-service teachers. A moderately positive relationship was found between AMO and EI, but it was observed that AMO is not a significant predictor of EI. A high positive relationship was detected between SE and EI; it was observed that SE explained 63% of the variance in EI. We found that there was no significant difference between the AMO, SE, and EI scores of pre-service teachers studying at different grade/class levels. As we continue to investigate these topics, it becomes evident that teacher education may continue promoting not only cognitive development but also emotional and social development. Full article
(This article belongs to the Special Issue Emotions, Emotion Mindsets, and Emotional Intelligence)
11 pages, 807 KiB  
Article
Impact of Robotic-Assisted Partial Nephrectomy with Single Layer versus Double Layer Renorrhaphy on Postoperative Renal Function
by Hiroyuki Ito, Keita Nakane, Noriyasu Hagiwara, Makoto Kawase, Daiki Kato, Koji Iinuma, Kenichiro Ishida, Torai Enomoto, Minori Nezasa, Yuki Tobisawa, Takayasu Ito and Takuya Koie
Curr. Oncol. 2024, 31(5), 2758-2768; https://doi.org/10.3390/curroncol31050209 (registering DOI) - 13 May 2024
Abstract
We aimed to investigate the differences in renal function between patients who underwent single inner-layer renorrhaphy (SILR) or double-layer renorrhaphy (DLR) among those with renal tumors who underwent robot-assisted partial nephrectomy (RAPN). This retrospective multicenter cohort study was conducted between November 2018 and [...] Read more.
We aimed to investigate the differences in renal function between patients who underwent single inner-layer renorrhaphy (SILR) or double-layer renorrhaphy (DLR) among those with renal tumors who underwent robot-assisted partial nephrectomy (RAPN). This retrospective multicenter cohort study was conducted between November 2018 and October 2023 at two institutions and included patients who underwent RAPN. In total, 93 eligible patients who underwent RAPN were analyzed. Preoperative renal function and prevalence of chronic kidney disease were not significantly different between the two groups. Although urinary leakage was observed in three patients (5.9%) in the SILR group, there was no significant difference between the two groups regarding surgical outcomes (p = 0.249). Serum creatinine levels after RAPN were significantly lower in the SILR group than in the DLR group on postoperative days 1 and 365 following RAPN (p = 0.04). The estimated glomerular filtration rate (eGFR) was significantly lower in the DLR group than in the SILR group only on postoperative day 1; however, there was no significant difference between the two groups thereafter. Multivariate analysis showed that the method of renorrhaphy was not a predictor for maintaining renal function after RAPN even though it was associated with eGFR on postoperative day 1. Full article
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14 pages, 1855 KiB  
Review
Diagnostic Biomarkers in Renal Cell Tumors According to the Latest WHO Classification: A Focus on Selected New Entities
by Francesca Sanguedolce, Roberta Mazzucchelli, Ugo Giovanni Falagario, Angelo Cormio, Magda Zanelli, Andrea Palicelli, Maurizio Zizzo, Albino Eccher, Matteo Brunelli, Andrea Benedetto Galosi, Giuseppe Carrieri and Luigi Cormio
Cancers 2024, 16(10), 1856; https://doi.org/10.3390/cancers16101856 (registering DOI) - 13 May 2024
Abstract
The fifth edition of the World Health Organization (WHO) classification for urogenital tumors, released in 2022, introduces some novelties in the chapter on renal epithelial tumors compared to the previous 2016 classification. Significant changes include the recognition of new disease entities and adjustments [...] Read more.
The fifth edition of the World Health Organization (WHO) classification for urogenital tumors, released in 2022, introduces some novelties in the chapter on renal epithelial tumors compared to the previous 2016 classification. Significant changes include the recognition of new disease entities and adjustments in the nomenclature for certain pathologies. Notably, each tumor entity now includes minimum essential and desirable criteria for reliable diagnosis. This classification highlights the importance of biological and molecular characterization alongside traditional cytological and architectural features. In this view, immunophenotyping through immunohistochemistry (IHC) plays a crucial role in bridging morphology and genetics. This article aims to present and discuss the role of key immunohistochemical markers that support the diagnosis of new entities recognized in the WHO classification, focusing on critical topics associated with single markers, in the context of specific tumors, such as the clear cell capillary renal cell tumor (CCPRCT), eosinophilic solid and cystic renal cell carcinoma (ESC-RCC), and so-called “other oncocytic tumors”, namely the eosinophilic vacuolated tumor (EVT) and low-grade oncocytic tumor (LOT). Their distinctive characteristics and immunophenotypic profiles, along with insights regarding diagnostic challenges and the differential diagnosis of these tumors, are provided. This state-of-the-art review offers valuable insights in biomarkers associated with novel renal tumors, as well as a tool to implement diagnostic strategies in routine practice. Full article
(This article belongs to the Special Issue New Insights into Urologic Oncology)
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13 pages, 263 KiB  
Article
Assessment of Executive Functions in Children with Sensorineural Hearing Loss and in Children with Specific Language Impairment: Preliminary Reports
by Maria Lauriello, Giulia Mazzotta, Antonella Mattei, Ilaria Mulieri, Alessandra Fioretti, Enzo Iacomino and Alberto Eibenstein
Brain Sci. 2024, 14(5), 491; https://doi.org/10.3390/brainsci14050491 (registering DOI) - 13 May 2024
Abstract
Executive functions (EFs) are related abilities, associated with the frontal lobes functions, that allow individuals to modify behavioral patterns when they become unsatisfactory. The aim of this study was to assess EFs in children with sensorineural hearing loss (SNHL) and in children with [...] Read more.
Executive functions (EFs) are related abilities, associated with the frontal lobes functions, that allow individuals to modify behavioral patterns when they become unsatisfactory. The aim of this study was to assess EFs in children with sensorineural hearing loss (SNHL) and in children with “specific language impairment” (SLI), compared with a control group of children with normal development, to identify specific skill deficits. Three groups of preschool children aged between 2 and 6 years were assessed: 19 children with normal hearing, cognitive, and language development, 10 children with SNHL, and 20 children with SLI. The FE-PS 2-6 Battery was used for the assessment of preschool EFs, supplemented with the Modified Bell Test for the analysis of selective attention. Statistically significant differences were found between the two experimental groups and the control one, regarding the investigated skills. Children with SNHL showed a clear deficit in flexibility, whereas children with SLI had greater problems in self-regulation and management of waiting for gratification. Selective attention was found to be deficient in all three groups, with no statistically significant differences. This study shows that the skills investigated were found to be deficient in both SNHL and SLI patients. It is essential to start targeted exercises based on specific deficient skills as part of the rehabilitation program. It is of great importance to understand the consequences of EF deficit in preschool children to achieve an accurate diagnosis and carry out customized rehabilitation programs. Full article
(This article belongs to the Special Issue Advances in Tinnitus and Hearing Disorders)
12 pages, 484 KiB  
Article
The Burden and Psychological Distress of Family Caregivers of Individuals with Autism Spectrum Disorder: A Gender Approach
by Raquel Herrero, Amelia Díaz and Jesús Zueco
J. Clin. Med. 2024, 13(10), 2861; https://doi.org/10.3390/jcm13102861 (registering DOI) - 13 May 2024
Abstract
Background/Objectives: Relatives play the main role as caregivers of autism spectrum disorder (ASD) individuals. Women, specifically mothers, are the majority of caregivers of ASD relatives. In addition, the literature on caregivers has shown that women have worse mental health and higher perceived burdens [...] Read more.
Background/Objectives: Relatives play the main role as caregivers of autism spectrum disorder (ASD) individuals. Women, specifically mothers, are the majority of caregivers of ASD relatives. In addition, the literature on caregivers has shown that women have worse mental health and higher perceived burdens than men. Therefore, the aim of this work was to evaluate the relationships between psychological distress and burden using a gender approach in caregivers of ASD relatives. Methods: A cross-sectional design was applied in this study with a convenience sample of 250 caregivers of ASD relatives. Most of them were mothers caring for a child who ranged in age from 1 to 31 years. Sociodemographic variables considered were age, education level, marital status, and relation to the care recipient. Additionally, psychological distress and objective burden, in the form of hours/day caring, and subjective burden, in the form of perceived burden, were analyzed. Results: Significant gender differences were found in psychological distress and objective and subjective burden, with women showing higher scores than men. Both types of burden played a serial mediating role between gender and psychological distress. Conclusions: The results highlight the important role of gender, with women bearing the high cost of caring for their children with ASD in the form of high objective burden, caring for more hours, and subjective burden, perceiving more burden and showing poorer mental health than men. These results show the need for specific support and intervention programs targeted to women caregivers to reduce burden and improve their mental health. Full article
(This article belongs to the Section Mental Health)
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21 pages, 7045 KiB  
Article
Fabrication of Multiple-Channel Electrochemical Microneedle Electrode Array via Separated Functionalization and Assembly Method
by Xin-Shuo Huang, Shuang Huang, Shan-Tao Zheng, Bao-Ming Liang, Tao Zhang, Wan Yue, Fan-Mao Liu, Peng Shi, Xi Xie and Hui-Jiuan Chen
Biosensors 2024, 14(5), 243; https://doi.org/10.3390/bios14050243 (registering DOI) - 13 May 2024
Abstract
Real-time monitoring of physiological indicators inside the body is pivotal for contemporary diagnostics and treatments. Implantable electrodes can not only track specific biomarkers but also facilitate therapeutic interventions. By modifying biometric components, implantable electrodes enable in situ metabolite detection in living tissues, notably [...] Read more.
Real-time monitoring of physiological indicators inside the body is pivotal for contemporary diagnostics and treatments. Implantable electrodes can not only track specific biomarkers but also facilitate therapeutic interventions. By modifying biometric components, implantable electrodes enable in situ metabolite detection in living tissues, notably beneficial in invasive glucose monitoring, which effectively alleviates the self-blood-glucose-managing burden for patients. However, the development of implantable electrochemical electrodes, especially multi-channel sensing devices, still faces challenges: (1) The complexity of direct preparation hinders functionalized or multi-parameter sensing on a small scale. (2) The fine structure of individual electrodes results in low spatial resolution for sensor functionalization. (3) There is limited conductivity due to simple device structures and weakly conductive electrode materials (such as silicon or polymers). To address these challenges, we developed multiple-channel electrochemical microneedle electrode arrays (MCEMEAs) via a separated functionalization and assembly process. Two-dimensional microneedle (2dMN)-based and one-dimensional microneedle (1dMN)-based electrodes were prepared by laser patterning, which were then modified as sensing electrodes by electrochemical deposition and glucose oxidase decoration to achieve separated functionalization and reduce mutual interference. The electrodes were then assembled into 2dMN- and 1dMN-based multi-channel electrochemical arrays (MCEAs), respectively, to avoid damaging functionalized coatings. In vitro and in vivo results demonstrated that the as-prepared MCEAs exhibit excellent transdermal capability, detection sensitivity, selectivity, and reproducibility, which was capable of real-time, in situ glucose concentration monitoring. Full article
(This article belongs to the Special Issue Recent Advances in Microneedle Array Electrodes in Biomedicine)
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14 pages, 1527 KiB  
Article
Impact of Nutrient Intake on Body Composition in Very Low-Birth Weight Infants Following Early Progressive Enteral Feeding
by Rasa Brinkis, Kerstin Albertsson-Wikland, Kastytis Šmigelskas, Aurika Vanckavičienė, Ilona Aldakauskienė, Rasa Tamelienė and Rasa Verkauskienė
Nutrients 2024, 16(10), 1462; https://doi.org/10.3390/nu16101462 (registering DOI) - 13 May 2024
Abstract
Preterm infants have increased body adiposity at term-equivalent age and risk of adverse metabolic outcomes. The aim of the study was to define how nutrient intake may impact body composition (BC) of very low-birth weight infants fed with early progressive enteral feeding and [...] Read more.
Preterm infants have increased body adiposity at term-equivalent age and risk of adverse metabolic outcomes. The aim of the study was to define how nutrient intake may impact body composition (BC) of very low-birth weight infants fed with early progressive enteral feeding and standard fortification. Eighty-six infants with <1500 g birth weight were included in the BC study and stratified into extremely preterm (EP) and very preterm (VP) groups. Nutrient intake was calculated during the first 28 days and BC assessed by dual X-ray absorptiometry at discharge and by skinfold thickness at 12 months of corrected age (CA). Total nutrient intake did not differ between the groups. EP infants had a higher fat mass percentage at discharge than VP infants (24.8% vs. 19.4%, p < 0.001); lean mass did not differ. None of the nutrients had any impact on BC of EP infants. Protein intake did not result in a higher lean mass in either group; fat intake was a significant predictor of increased fat mass percentage in VP infants at discharge (p = 0.007) and body adiposity at 12 months of CA (p = 0.021). Nutritional needs may depend on gestational age and routine fortification should be used with caution in more mature infants. Full article
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16 pages, 293 KiB  
Review
The Potential Systemic Role of Diet in Dental Caries Development and Arrest: A Narrative Review
by Ashley J. Malin, Zhilin Wang, Durdana Khan and Sarah L. McKune
Nutrients 2024, 16(10), 1463; https://doi.org/10.3390/nu16101463 (registering DOI) - 13 May 2024
Abstract
Current conceptualizations of dental caries etiology center primarily on the local role of sugar, starch, or other fermentable carbohydrates on tooth enamel demineralization—a well-established and empirically supported mechanism. However, in addition to this mechanism, studies dating back to the early 1900s point to [...] Read more.
Current conceptualizations of dental caries etiology center primarily on the local role of sugar, starch, or other fermentable carbohydrates on tooth enamel demineralization—a well-established and empirically supported mechanism. However, in addition to this mechanism, studies dating back to the early 1900s point to an important systemic role of diet and nutrition, particularly from pasture-raised animal-source foods (ASF), in dental caries etiology and arrest. Findings from animal and human studies suggest that adherence to a diet high in calcium, phosphorus, fat-soluble vitamins A and D, and antioxidant vitamin C, as well as low in phytates, may contribute to arrest and reversal of dental caries, particularly among children. Furthermore, findings from observational and experimental studies of humans across the life-course suggest that fat-soluble vitamins A, D, and K2 may interact to protect against dental caries progression, even within a diet that regularly contains sugar. While these historic studies have not been revisited in decades, we emphasize the need for them to be reinvestigated and contextualized in the 21st century. Specifically, methodologically rigorous studies are needed to reinvestigate whether historical knowledge of systemic impacts of nutrition on dental health can help to inform current conceptualizations of dental caries etiology, prevention, and arrest. Full article
12 pages, 1402 KiB  
Article
Is the Tendon-to-Groove Ratio Associated with Elevated Risk for LHB Tendon Disorders?—A New Approach of Preoperative MR-Graphic Analysis for Targeted Diagnosis of Tendinopathy of the Long Head of Biceps
by Kristina Gerhardinger, Lisa Klute, Christian Pfeifer, Josina Straub, Laura Hechinger, Moritz Riedl, Volker Alt, Maximilian Kerschbaum and Leopold Henssler
J. Clin. Med. 2024, 13(10), 2860; https://doi.org/10.3390/jcm13102860 (registering DOI) - 13 May 2024
Abstract
Background: Pathologies of the long head of the biceps (LHB) tendon are a common cause of anterior shoulder pain. While the influence of the anatomical morphology of the intertubercular groove (ITG) on the development of LHB tendon instability has been investigated with [...] Read more.
Background: Pathologies of the long head of the biceps (LHB) tendon are a common cause of anterior shoulder pain. While the influence of the anatomical morphology of the intertubercular groove (ITG) on the development of LHB tendon instability has been investigated with ambiguous results, the relationship of the LHB to ITG anatomy has not yet been considered in this context. The objective of this study was to reliably extract the tendon-to-groove ratio from MRI scans of symptomatic patients and examine its potential influence on the occurrence of certain causes for LHB-associated symptoms. Methods: In this retrospective study, preoperative MRI scans of 35 patients (mean age of 46 ± 14 years) presenting with anterior shoulder pain and clinical indications of LHB tendinopathy were analyzed in transversal planes. Long and short diameters of the LHB tendon and ITG were measured, cross-sectional areas of the LHB tendon and ITG were calculated from these measurements, and the ratio of cross-sectional areas (LHB/ITG) was introduced. All measurements were repeated independently by three investigators and inter-rater reliability was assessed using intraclass correlation coefficient (ICC). Thereafter, tendon-to-groove ratios were compared in patients with and without intraoperative signs of LHB tendon instability. Results: All patients exhibited intraoperative signs of LHB tendinitis, with additional findings including pulley lesions and SLAP lesions. Analysis revealed variations in the dimensions of the LHB tendon and ITG cross sections, with the tendon-to-groove ratio decreasing from 37% at the pulley to 31% at the deepest point of the sulcus. Very good inter-rater reliability was observed for all measurements. The tendon-to-groove ratio did not significantly differ (p > 0.05) in patients with or without pulley lesions or SLAP lesions. Conclusions: Our study introduced the novel parameter of the tendon-to-groove ratio of cross-sectional areas as a reproducible parameter for the description of local anatomy in the field of targeted diagnosis of LHB tendon disorders. While our findings do not yet support the predictive value of the tendon-to-groove ratio, they underscore the importance of further research with larger cohorts and control groups to validate these observations. Full article
(This article belongs to the Special Issue Targeted Diagnosis and Treatment of Shoulder and Elbow Disease)
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13 pages, 663 KiB  
Article
Meat Characteristics, Expression of Myosin Heavy Chain and Metabolism-Related Genes in Thai Native Pigs
by Chanporn Chaosap, Kamon Chaweewan, Kazeem D. Adeyemi, Netanong Phonkate and Ronachai Sitthigripong
Foods 2024, 13(10), 1502; https://doi.org/10.3390/foods13101502 (registering DOI) - 13 May 2024
Abstract
This study investigated the meat quality, expression of myosin heavy chain (MyHC) and metabolism-related genes, ribonucleotides and fatty acids in Longissimus thoracis of Thai native pigs (TNPs) from different geographical regions (GR). Forty-one 9–10-month-old castrated TNPs (BW 60 kg), consisting of 18, 11 [...] Read more.
This study investigated the meat quality, expression of myosin heavy chain (MyHC) and metabolism-related genes, ribonucleotides and fatty acids in Longissimus thoracis of Thai native pigs (TNPs) from different geographical regions (GR). Forty-one 9–10-month-old castrated TNPs (BW 60 kg), consisting of 18, 11 and 12 pigs from Northern (NT), Southern (ST) and Northeastern (NE) regions, respectively, were slaughtered. GR did not affect (p > 0.05) the expression of MyHC, phosphoglycerate mutase 1, cytosolic glycerol-3-phosphate dehydrogenase, triosephosphate isomerase 1 and adipocyte fatty acid binding protein genes. The trend of MyHC was MyHC IIx > MyHC IIb > MyHC IIa > MyHC I. The NT loin had higher (p < 0.05) glycogen, C18:2n6, C20:4n6 and cooking loss, lower inosine, inosine monophosphate and hypoxanthine and a shorter sarcomere length than the ST and NE loins. The ST loin had a lower (p < 0.05) a* compared to other loins. Principal component analysis established significant relationships between the TNP and specific meat quality traits. This finding suggests that GR affected the meat quality, ribonucleotides and selected fatty acids in TNPs. These results provide relevant information that can be used to optimize the use of Thai native pork. Full article
(This article belongs to the Section Meat)
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12 pages, 2026 KiB  
Article
Experimental Investigation of Impulsive Coupling Characteristics of Asteroid Simulants Based on Laser Ablation Propulsion
by Yingjie Ma, Hao Chang, Weijing Zhou and Zhilong Jian
Aerospace 2024, 11(5), 388; https://doi.org/10.3390/aerospace11050388 (registering DOI) - 13 May 2024
Abstract
The ablation impulse of typical asteroid simulants irradiated by a nanosecond pulsed laser has been investigated in a vacuum environment. A torsional pendulum measurement system was constructed to calculate the impulse of laser ablation. A 10 ns pulsed laser was used, with a [...] Read more.
The ablation impulse of typical asteroid simulants irradiated by a nanosecond pulsed laser has been investigated in a vacuum environment. A torsional pendulum measurement system was constructed to calculate the impulse of laser ablation. A 10 ns pulsed laser was used, with a 1064 nm wavelength, a 900 mJ maximum pulse energy, and a millimeter-scale ablation spot diameter. Impulsive coupling characteristics of six typical targets that imitate the substance of asteroids with various laser fluences were analyzed. Furthermore, the impulse coupling coefficient curves of different materials were fitted. The results reveal that the minimum laser fluence corresponding to a measurable ablation impulse is approximately 2.5 J/cm2, and the optimum laser fluence corresponding to the maximum impulse coupling coefficient is approximately 14.0 J/cm2. The trends of the laser ablation impulse coupling curves are roughly consistent for the six materials. Impulse coupling characteristics of the six typical materials can be represented by the same polynomial within a 95% confidence interval, so a unified rule has been given. In actual deflection tasks of asteroids, the unified impulse coupling characteristic can be used to implement laser deflection techniques, especially when the material of the asteroid cannot be accurately judged in time. Full article
(This article belongs to the Special Issue Laser Propulsion Science and Technology)
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15 pages, 2699 KiB  
Article
Microbial Biomass and Rhizosphere Soil Properties in Response to Heavy Metal-Contaminated Flooding
by Tibor Szili-Kovács and Tünde Takács
Agriculture 2024, 14(5), 756; https://doi.org/10.3390/agriculture14050756 (registering DOI) - 13 May 2024
Abstract
Mining and metallurgy are the main sources of soil contamination with harmful metals, posing a significant threat to human health and ecosystems. River floodplains in the vicinity of metal mines or industrial plants are often subject to flooding with sediments containing heavy metals, [...] Read more.
Mining and metallurgy are the main sources of soil contamination with harmful metals, posing a significant threat to human health and ecosystems. River floodplains in the vicinity of metal mines or industrial plants are often subject to flooding with sediments containing heavy metals, which can be harmful to the soil ecosystem. This study aimed to investigate the microbial properties of the soil at a metal-contaminated site and to determine the significant relationships between the biological and chemical properties of the soil. The study site was located near the village of Gyöngyösoroszi, in the Mátra mountain region of northwest Hungary. A phytoremediation experiment was conducted in a metal-polluted floodplain using willow and corn plantations. The soil basal respiration, substrate-induced respiration, soil microbial biomass carbon (MBC), acid phosphatase activities, and soil chemical properties were measured. The soil of the contaminated sites had significantly higher levels of As, Pb, Zn, Cu, Cd, and Ca, whereas the unpolluted sites had significantly higher levels of phosphorus and potassium. The substrate-induced respiration showed a positive correlation with MBC and negative correlations with the metabolic quotient (qCO2). The soil plasticity index and phosphorus showed a positive correlation with MBC, whereas salinity and the presence of Cd, Pb, Zn, As, and Cu showed a negative correlation. Acid phosphomonoesterase activity negatively correlated with the plant-available phosphorus content and MBC, but was positively correlated with the contents of toxic elements, including cadmium, lead, zinc, arsenic, and copper. This study found a significant correlation between the qCO2 and the toxic element content. This suggests that an enhanced metabolic quotient (qCO2), together with a decreased MBC/SOC ratio, could be used to indicate the harmful effect of soil contamination by heavy metals in floodplain soils. Full article
(This article belongs to the Special Issue Advanced Research of Rhizosphere Microbial Activity—Series II)
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