This case concentrates on the persistent left superior vena cava (PLSVC), a rare vascular anomaly which contributes to central venous catheter (CVC) misplacement. A 72-year-old woman with renal insufficiency presented to the hospital with recurrent bleeding from her permanent CVC device placed in the right common jugular vein. An initial attempt to replace the device was unsuccessful, necessitating the placement of a secondary catheter in the left jugular vein. Shortly after the procedure, the patient developed swelling of the face and neck. Further diagnostic imaging, including a chest radiograph and computed tomography (CT), revealed CVC misplacement in the PLSVC and coronary sinus, thrombosis of the common jugular vein, and a posterior mediastinal hematoma. Conservative therapy of the mediastinal hematoma was implemented and proved effective in this case. A temporary CVC was inserted into the left femoral vein. Two months later, the catheter underwent further dysfunction and a decision was made to place a long-term permanent CVC via the right femoral vein. The patient is currently awaiting an arteriovenous fistula for dialysis use. This case emphasizes the importance of radiological techniques for CVC procedural placement, as well as the detection of congenital abnormalities. Providers regularly placing CVCs should have an in-depth knowledge of the possible complications and potential anatomical variations, especially as seen in high-risk patients. Full article

The repeated failure to treat patients chronically infected with hepatitis E (HEV) and C (HCV) viruses, despite the absence of resistance-associated substitutions (RAS), particularly in response to prolonged treatments with the mutagenic agents of HEV, suggests that quasispecies structure may play a crucial role beyond single point mutations. Quasispecies structured in a flat-like manner (referred to as flat-like) are considered to possess high average fitness, occupy a significant fraction of the functional genetic space of the virus, and exhibit a high capacity to evade specific or mutagenic treatments. In this paper, we studied HEV and HCV samples using high-depth next-generation sequencing (NGS), with indices scoring the different properties describing flat-like quasispecies. The significance of these indices was demonstrated by comparing the values obtained from these samples with those from acute infections caused by respiratory viruses (betacoronaviruses, enterovirus, respiratory syncytial viruses, and metapneumovirus). Our results revealed that flat-like quasispecies in HEV and HCV chronic infections without RAS are characterized by numerous low-frequency haplotypes with no dominant one. Surprisingly, these low-frequency haplotypes (at the nucleotide level) exhibited a high level of synonymity, resulting in much lower diversity at the phenotypic level. Currently, clinical approaches for managing flat-like quasispecies are lacking. Here, we propose methods to identifying flat-like quasispecies, which represents an essential initial step towards exploring alternative treatment protocols for viruses resistant to conventional therapies. Full article

This study investigates the mechanical properties of corroded Q355B structural steel subjected to a simulated marine atmosphere and an industrial atmosphere. The micro-morphology of corroded steel in two different environments was analyzed by SEM (scanning electron microscopy). Tension tests were performed to determine the degradation laws of the mechanical properties of corroded steel, including its yield strength, ultimate strength, elastic modulus, ultimate strain and elongation after fracture. The test results indicate that the elongation after fracture of the steel is the most severely deteriorated property after corrosion. The recommended empirical formula for limiting the maximum corrosion rate is established. It is found that when the initial elongation is 30%, the maximum allowable corrosion rate is 19.2%. Based on the achieved results, a simplified time-dependent stress–strain model of Q355B structural steel is established considering the coupling effects of corrosive environments and applied stress, which is also evaluated using relevant research. In addition, axial compression tests were conducted on corroded square stud columns to verify the effectiveness of the established model. It is indicated that the model can be used for fitness-for-purpose analyses in structural integrity assessments. Full article

The SARS-CoV-2 Omicron variants have replaced all earlier variants, due to increased infectivity and effective evasion from infection- and vaccination-induced neutralizing antibodies. Compared to earlier variants of concern (VoCs), the Omicron variants show high TMPRSS2-independent replication in the upper airway organs, but lower replication in the lungs and lower mortality rates. The shift in cellular tropism and towards lower pathogenicity of Omicron was hypothesized to correlate with a lower toll-like receptor (TLR) activation, although the underlying molecular mechanisms remained undefined. In silico analyses presented here indicate that the Omicron spike protein has a lower potency to induce dimerization of TLR4/MD-2 compared to wild type virus despite a comparable binding activity to TLR4. A model illustrating the molecular consequences of the different potencies of the Omicron spike protein vs. wild-type spike protein for TLR4 activation is presented. Further analyses indicate a clear tendency for decreasing TLR4 dimerization potential during SARS-CoV-2 evolution via Alpha to Gamma to Delta to Omicron variants. Full article

Electron beam–powder bed fusion (PBF-EB) is an additive manufacturing process that utilizes an electron beam as the heat source to enable material fusion. However, the use of a charge-carrying heat source can sometimes result in sudden powder explosions, usually referred to as “Smoke”, which can lead to process instability or termination. This experimental study investigated the initiation and propagation of Smoke using in situ high-speed synchrotron radiography. The results reveal two key mechanisms for Smoke evolution. In the first step, the beam–powder bed interaction creates electrically isolated particles in the atmosphere. Subsequently, these isolated particles get charged either by direct irradiation by the beam or indirectly by back-scattered electrons. These particles are accelerated by electric repulsion, and new particles in the atmosphere are produced when they impinge on the powder bed. This is the onset of the avalanche process known as Smoke. Based on this understanding, the dependence of Smoke on process parameters such as beam returning time, beam diameter, etc., can be rationalized. Full article

Prostate cancer is frequently diagnosed and the leading cause of death in men worldwide. Prostate-specific antigen (PSA) blood tests and biopsies are the primary methods for diagnosing prostate cancer; however, their accuracy is less than 50%. Therefore, there is a need to develop diagnostic tests that minimize patient discomfort during examination and adequate biomarkers that are more accurate, sensitive, and specific for the detection of prostate cancer. This study investigated the application of metabolomics to identify biomarkers in prostate cancer biofluids. In addition, changes in prostate cancer metabolite levels induced by chrysin, a natural anticancer compound, were evaluated and compared with those in non-treated prostate cancer cells. Gas chromatography-mass spectrometry (GC-MS)-based metabolomic profiling was performed to investigate the differences in metabolic alterations among prostate cancer, normal prostate, and chrysin-treated prostate cancer cells. Pairwise comparisons of the extracellular fluid metabolomes were performed using principal component analysis (PCA), partial least squares–discriminant analysis (PLS-DA), and Student’s t-test. The results revealed significantly different patterns among the metabolite groups, including alcohols, amino acids, carboxylic acids, organic acids, sugars, and urea. The RWPE-1- and chrysin-treated PC-3 (PC-3 Chr) cell groups showed similar tendencies for 23 metabolites, while the groups showed significant differences from the PC-3 group. Most amino acids showed higher concentrations in PC-3 cells than in the normal cell line RWPE-1 cells and PC-3 Chr cells. Our results revealed that GC-MS might be an effective diagnostic tool to detect prostate cancer and contribute to finding new tumor markers for prostate cancer as the basis for new ideas. Full article

Nanoplastics, created by the fragmentation of larger plastic debris, are a serious pollutant posing substantial environmental and health risks. Here, we developed a polystyrene nanoparticle (PS-NP) exposure model during mice pregnancy to explore their effects on embryonic development. We found that exposure to 30 nm PS-NPs during pregnancy resulted in reduced mice placental weight and abnormal embryonic development. Subsequently, our transcriptomic dissection unveiled differential expression in 102 genes under PS-NP exposure and the p38 MAPK pathway emerged as being significantly altered in KEGG pathway mapping. Our findings also included a reduction in the thickness of the trophoblastic layer in the placenta, diminished cell invasion capabilities, and an over-abundance of immature red cells in the blood vessels of the mice. In addition, we validated our findings through the human trophoblastic cell line, HTR-8/SVneo (HTR). PS-NPs induced a drop in the vitality and migration capacities of HTR cells and suppressed the p38 MAPK signaling pathway. This research highlights the embryotoxic effects of nanoplastics on mice, while the verification results from the HTR cells suggest that there could also be certain impacts on the human trophoblast layer, indicating a need for further exploration in this area. Full article

α-CD:N₂O “host-guest” type complexes were formed by a simple solid–gas reaction (N₂O sorption into α-CD) under different gas pressures and temperatures. The new N₂O inclusion method applied in the present study was compared with the already known technique based on the crystallization of clathrates from a water solution of α-CD saturated with N₂O. A maximum storage capacity of 4.5 wt.% N₂O was achieved when charging the cyclodextrin from a gas phase. The amount of included gas decreases to 1.3 wt.% when the complex is stored in air at 1 atm and room temperature, analogous to that achieved by the crystallization of α-CD:N₂O. Furthermore, it was shown that the external coordination of N₂O to either the upper or lower rim of α-CD without hydration water displacement is the preferred mode of binding, due to hydrogen bonds with neighboring -OH groups from the host macrocycle and three of the hydration water molecules nearby. The capacity of α-CD to store N₂O and the thermal stability of the α-CD:N₂O complex demonstrated promising applications of these types of complexes in food and beverages. Full article

The construction industry, business models, and smart cities are recognized as pivotal domains with profound implications for fostering sustainability, prompting extensive research endeavors. However, there remains a dearth of interdisciplinary integration within this sphere aimed at fostering sustainable development. Nevertheless, current studies suggest that research in this area could provide theoretical and practical guidance for the sustainable transformation of society and make a positive contribution to the realization of the Sustainable Development Goals (SDGs). Therefore, this paper aims to utilize an innovative mixed research approach combining macro-quantitative bibliometric analysis with subsequent micro-qualitative content examination based on the SDGs to explore the relationship between BIM and smart cities in promoting a sustainability-oriented business model, which provides a comprehensive understanding of the overall situation and development of research topics in the field and contributes to the improvement of the SDGs. The results show that, during the last 13 years (from the year 2011 to 2023), the period from the year 2011 to 2016 was the initial stage of the field, followed by a rapid growth after the year 2018, of which “BIM”, “Smart City”, “Business Model”, “Building Life Cycle”, “Urban Management”, and “Business Model Innovation” are the keywords representing the current research hotspots. The circular economy model that has been developed since 2021 has contributed to life cycle stages, including “briefing stages” and “procurement stages”. As such, the “whole life cycle”, “strategic urban planning frameworks”, and “sustainable business models” have become future research trends, whilst real-world applications such as “smart tourism”, “e-government”, and “green building” have emerged. Further, the key partnerships of “city managers”, “corporate enterprises”, and “public participation” for smart cities contribute to the achievement of SDGs 8 and 17 in terms of integrating urban information technology and urban infrastructure, policy regulation, knowledge-sharing, improving economic efficiency, and promoting sustainable economic growth. Full article

Fibrillin-1 and fibrillin-2, encoded by FBN1 and FBN2, respectively, play significant roles in elastic fiber assembly, with pathogenic variants causing a diverse group of connective tissue disorders such as Marfan syndrome (MFS) and congenital contractural arachnodactyly (CCD). Different genomic variations may lead to heterogeneous phenotypic features and functional consequences. Recent high-throughput sequencing modalities have allowed detection of novel variants that may guide the care for patients and inform the genetic counseling for their families. We performed clinical phenotyping for two newborn infants with complex congenital heart defects. For genetic investigations, we employed next-generation sequencing strategies including whole-genome Single-Nucleotide Polymorphism (SNP) microarray for infant A with valvular insufficiency, aortic sinus dilatation, hydronephrosis, and dysmorphic features, and Trio whole-exome sequencing (WES) for infant B with dextro-transposition of the great arteries (D-TGA) and both parents. Infant A is a term male with neonatal marfanoid features, left-sided hydronephrosis, and complex congenital heart defects including tricuspid regurgitation, aortic sinus dilatation, patent foramen ovale, patent ductus arteriosus, mitral regurgitation, tricuspid regurgitation, aortic regurgitation, and pulmonary sinus dilatation. He developed severe persistent pulmonary hypertension and worsening acute hypercapnic hypoxemic respiratory failure, and subsequently expired on day of life (DOL) 10 after compassionate extubation. Cytogenomic whole-genome SNP microarray analysis revealed a deletion within the FBN1 gene spanning exons 7–30, which overlapped with the exon deletion hotspot region associated with neonatal Marfan syndrome. Infant B is a term male prenatally diagnosed with isolated D-TGA. He required balloon atrial septostomy on DOL 0 and subsequent atrial switch operation, atrial septal defect repair, and patent ductus arteriosus ligation on DOL 5. Trio-WES revealed compound heterozygous c.518C>T and c.8230T>G variants in the FBN2 gene. Zygosity analysis confirmed each of the variants was inherited from one of the parents who were healthy heterozygous carriers. Since his cardiac repair at birth, he has been growing and developing well without any further hospitalization. Our study highlights novel FBN1/FBN2 variants and signifies the phenotype–genotype association in two infants affected with complex congenital heart defects with and without dysmorphic features. These findings speak to the importance of next-generation high-throughput genomics for novel variant detection and the phenotypic variability associated with FBN1/FBN2 variants, particularly in the neonatal period, which may significantly impact clinical care and family counseling. Full article

Machine learning (ML) algorithms have been widely used to improve the performance of telecommunications systems, including reconfigurable intelligent surface (RIS)-assisted wireless communication systems. The RIS can be considered a key part of the backbone of sixth-generation (6G) communication mainly due to its electromagnetic properties for controlling the propagation of the signals in the wireless channel. The ML-optimized (RIS)-assisted wireless communication systems can be an effective alternative to mitigate the degradation suffered by the signal in the wireless channel, providing significant advantages in the system’s performance. However, the variety of approaches, system configurations, and channel conditions make it difficult to determine the best technique or group of techniques for effectively implementing an optimal solution. This paper presents a comprehensive review of the reported frameworks in the literature that apply ML and RISs to improve the overall performance of the wireless communication system. This paper compares the ML strategies that can be used to address the RIS-assisted system design. The systems are classified according to the ML method, the databases used, the implementation complexity, and the reported performance gains. Finally, we shed light on the challenges and opportunities in designing and implementing future RIS-assisted wireless communication systems based on ML strategies. Full article

Background/Objectives: Population aging is rapidly increasing, and the importance of preventive medicine has been stressed. Health checkups, diet, and exercise are of paramount importance. This study aimed to evaluate the effectiveness of a personalized dual-task intervention that combined exercise with cognitive tasks in improving physical and cognitive functions among independently living older individuals. Methods: Participants aged >65 years who were mostly independent in their activities of daily living were divided into two groups. The group receiving the 20 min robot-assisted session was compared with the group receiving traditional functional restoration training. This randomized trial assessed the impact of this intervention on the 30 s chair stand test score and Montreal Cognitive Assessment—Japanese version score of the participants. Results: Both scores significantly improved in the intervention group, indicating enhanced lower-limb function and cognitive capabilities. Conclusions: These findings suggest that integrating cognitive tasks with physical exercise can stand as an effective strategy to improve overall well-being in older people, offering valuable insights for designing comprehensive preventive health programs tailored to this demographic. Full article

In 1926, a New York Times article described the cultural and ethnic flows in south Brazil as a “Melting Pot”. The report predicted that German Brazilians, tied to their ethnoracial origin, would soon be Brazilianized. The study of congregational song practices offers insight into the relationship between migration, race, culture, and ethnicity. Moreover, investigating Brazilian Lutheran singing practices helps us understand how the New York Times’ prediction unfolded on the ground. This paper examines the Brazilian Lutheran hymnal Livro de Canto, published in 2017, and displays how Brazil’s ethnoracial diversity is manifested and negotiated in the Lutheran context, both musically and theologically. By interviewing members of the hymnal committee and investigating how they dealt with Brazil’s ethnoraciality in the context of the hymnal compilation, this paper demonstrates ways denominations and churchgoers negotiate theological, cultural, musical, and ethnoracial identities through congregational singing. More importantly, it showcases how Brazilian Lutheran church music practices inform broader social conversations around racism, nationalism, Blackness, and Brazilianness. Full article

Respiratory syncytial virus (RSV) bronchiolitis remains a significant global health burden, particularly in newborns and infants during their first year of life. The quest for an effective preventive strategy against RSV has long been sought, and recent developments have shown promise in the form of nirsevimab, a monoclonal antibody specifically designed for RSV prophylaxis. Valle d’Aosta was the first Italian region to propose universal prophylaxis with nirsevimab for newborns and infants in their first epidemic season as early as 2023–2024. This study describes the effectiveness and safety of the universal prevention program of RSV bronchiolitis using the monoclonal antibody nirsevimab in children resident in Valle d’Aosta born during the 2023–2024 epidemic season. There were 556 neonates born from 1 May 2023 to 15 February 2024. The risk of hospitalization for RSV bronchiolitis in 2023–2024 was 3.2%, compared to 7% in the 2022–2023 epidemic season (p < 0.001). After the start of the prophylaxis campaign with nirsevimab, the risk of hospitalization was 8.3% in the sample of infants who did not adhere to the prophylaxis, while no child in the sample of those treated (p < 0.001) was hospitalized for bronchiolitis. Few mild transient side effects were reported. This study shows the efficacy and safety of universal prophylaxis with nirsevimab in neonates, making Valle d’Aosta the first Italian region to offer universal prophylaxis to newborns without risk factors for RSV complications. Future research could further explore its long-term impact and cost-effectiveness. Full article

As the economic burden associated with vision loss and ocular damage continues to rise, there is a need to explore novel treatment strategies. Extracellular vesicles (EVs) are enriched with various biological cargo, and there is abundant literature supporting the reparative and immunomodulatory properties of stem cell EVs across a broad range of pathologies. However, one area that requires further attention is the reparative effects of stem cell EVs in the context of ocular damage. Additionally, most of the literature focuses on EVs isolated from primary stem cells; the use of EVs isolated from human telomerase reverse transcriptase (hTERT)-immortalized stem cells has not been thoroughly examined. Using our large-scale EV-manufacturing platform, we reproducibly manufactured EVs from hTERT-immortalized mesenchymal stem cells (MSCs) and employed various methods to characterize and profile their associated cargo. We also utilized well-established cell-based assays to compare the effects of these EVs on both healthy and damaged retinal pigment epithelial cells. To the best of our knowledge, this is the first study to establish proof of concept for reproducible, large-scale manufacturing of hTERT-immortalized MSC EVs and to investigate their potential reparative properties against damaged retinal cells. The results from our studies confirm that hTERT-immortalized MSC EVs exert reparative effects in vitro that are similar to those observed in primary MSC EVs. Therefore, hTERT-immortalized MSCs may represent a more consistent and reproducible platform than primary MSCs for generating EVs with therapeutic potential. Full article

National parks play a crucial role in the worldwide system of natural conservation, significantly influencing land utilization, the sustainability of local livelihoods, and the environmental integrity of nearby regions. In this study, the coupled coordination model and ArcGIS tools are applied to examine the temporal and spatial progression of the coordination of the system of land, the environment, and residents’ livelihoods (the LEL system). The construction of the LEL system and the use of natural geographic and socio-economic data are the main innovations of this research. The findings indicate that over the last 15 years, Wuyishan National Park has witnessed growth in its ecological and agricultural areas alongside a decline in its water-based ecological zones. The creation of the national park has obviously promoted the coordination of the LEL systems in nearby areas and has a significant spatial spillover effect. Consequently, for the upcoming development of Wuyishan National Park, it is crucial to modify the framework of the agricultural sector, enhance local public infrastructure, and bolster the safeguarding of water ecological areas. Full article

Perfluorooctanoic acid (PFOA) is a globally prevalent contaminant of concern recognised for its persistence and detrimental effects on both wildlife and humans. While PFOA has been established as a disruptor of thyroid function, limited data exist regarding its impact on thyroid morphology. The kidney of the common carp (Cyprinus carpio) harbours numerous thyroid follicles, rendering it a valuable biomarker organ for investigating PFOA-induced thyroid alterations. Renal tissue slides, stained with the Alcian blue/PAS method, were examined from carp in three experimental groups: unexposed, exposed to 200 ng L⁻¹, and exposed to 2 mg L⁻¹ of PFOA over 56 days. Thyroid follicle colloids were segmented, and related morphometric parameters, including perimeter, area, and shape descriptors, were obtained. Statistical analyses revealed significant reductions in thyroid follicle colloid perimeter and area in the 200 ng L⁻¹ PFOA group compared to the unexposed and 2 mg L⁻¹ PFOA groups. Additionally, the fish exposed to PFOA exhibited a significantly higher follicle count compared to the unexposed fish. These findings collectively suggest that PFOA induces thyroid folliculogenesis, emphasising its impact on thyroid morphology even at an environmentally relevant concentration (200 ng L⁻¹). Full article

In this article, deployable cylindrical vaults of reciprocal linkages with frustoconical ends are analysed. Deployable cylindrical vaults with quadrangular scissors modules have low stiffness in the longitudinal direction, which requires the use of stiffening bars after deployment. The truncated cone-shaped ends improve their stiffness but do not prevent bracing. However, if reciprocal knots are used, the mesh performance improves considerably. This article studies the design conditions of these vaults and their resistance to gravity, wind suction and wind loads in the transverse and longitudinal directions. We also study the different resistance behaviour of the mesh, depending on whether the roof is supported on the upper or lower joints. In all cases, model tests are carried out to check the validity of the proposed solutions. Both theoretical calculations and experimental tests demonstrate the viability and effectiveness of this type of structure. Full article

The impact of romantic relationships during the adolescent period has received significantly less attention in the literature compared to the influence of romantic relationships during adulthood. Specifically, how these influences may differ between females and males. As such, the current study uses four waves of data from the Pathways to Desistance study to examine how elements of romantic relationships, including relationship quality, monitoring, and antisocial influence, affect adolescent delinquent behaviors by sex. Results indicated several important findings regarding the desistance effects of romantic relationship quality, monitoring, and antisocial influence on adolescent violent and non-violent offending, and these varied widely by sex. Limitations, future research, and policy recommendations are discussed. Full article

This white paper explores the detectability of intermediate-mass black holes (IMBHs) wandering in the Milky Way (MW) and massive local galaxies, with a particular emphasis on the role of AXIS. IMBHs, ranging within ${10}^{3-6}{\mathrm{M}}_{\odot }$, are commonly found at the centers of dwarf galaxies and may exist, yet undiscovered, in the MW. By using model spectra for advection-dominated accretion flows (ADAFs), we calculated the expected fluxes emitted by a population of wandering IMBHs with masses of ${10}^5{\mathrm{M}}_{\odot }$ in various MW environments and extrapolated our results to massive local galaxies. Around $40\%$ of the potential population of wandering IMBHs in the MW can be detected in an AXIS deep field. We proposed criteria to aid with selecting IMBH candidates using already available optical surveys. We also showed that IMBHs wandering in >200 galaxies within 10 Mpc can be easily detected with AXIS when passing within dense galactic environments (e.g., molecular clouds and cold neutral medium). In summary, we highlighted the potential X-ray detectability of wandering IMBHs in local galaxies and provided insights for guiding future surveys. Detecting wandering IMBHs is crucial for understanding their demographics and evolution and the merging history of galaxies. This white paper is part of a series commissioned for the AXIS Probe Concept Mission; additional AXIS white papers can be found at the AXIS website, with a mission overview here. Full article

This study examines the relationship between the business sentiment of Japanese companies regarding promising or potential countries for investment and macroeconomic statistics, such as economic or population growth in Thailand, using data from the Survey Report on Overseas Business Operations by Japanese Manufacturing Companies from 1992 to 2022. Although investing in Thailand has been popular among Japanese companies since the late 1980s, it has seemingly become relatively inactive in recent years. The present study’s results are summarized as follows: First, the business sentiment of Japanese companies has some relationships with relatively short-term economic growth and the business cycle in the short run. Second, business sentiment depends on long-term trends, and this stance may have changed after 2020. Third, other elements, such as minimum wage or fewer young people, do not necessarily have a relationship with business sentiment. Although more studies including capital accumulation or the global value chain should be conducted, improving the sentiments of Japanese businesspersons is desirable. Full article

Cryogenic electron tomography (cryoET) is a powerful tool in structural biology, enabling detailed 3D imaging of biological specimens at a resolution of nanometers. Despite its potential, cryoET faces challenges such as the missing wedge problem, which limits reconstruction quality due to incomplete data collection angles. Recently, supervised deep learning methods leveraging convolutional neural networks (CNNs) have considerably addressed this issue; however, their pretraining requirements render them susceptible to inaccuracies and artifacts, particularly when representative training data is scarce. To overcome these limitations, we introduce a proof-of-concept unsupervised learning approach using coordinate networks (CNs) that optimizes network weights directly against input projections. This eliminates the need for pretraining, reducing reconstruction runtime by 3–20× compared to supervised methods. Our in silico results show improved shape completion and reduction of missing wedge artifacts, assessed through several voxel-based image quality metrics in real space and a novel directional Fourier Shell Correlation (FSC) metric. Our study illuminates benefits and considerations of both supervised and unsupervised approaches, guiding the development of improved reconstruction strategies. Full article