The 2023 MDPI Annual Report has
been released!
 
33 pages, 2586 KiB  
Article
A Computational Model for the Simulation of Prepulse Inhibition and Its Modulation by Cortical and Subcortical Units
by Thiago Ohno Bezerra, Antonio C. Roque and Cristiane Salum
Brain Sci. 2024, 14(5), 502; https://doi.org/10.3390/brainsci14050502 (registering DOI) - 15 May 2024
Abstract
The sensorimotor gating is a nervous system function that modulates the acoustic startle response (ASR). Prepulse inhibition (PPI) phenomenon is an operational measure of sensorimotor gating, defined as the reduction of ASR when a high intensity sound (pulse) is preceded in milliseconds by [...] Read more.
The sensorimotor gating is a nervous system function that modulates the acoustic startle response (ASR). Prepulse inhibition (PPI) phenomenon is an operational measure of sensorimotor gating, defined as the reduction of ASR when a high intensity sound (pulse) is preceded in milliseconds by a weaker stimulus (prepulse). Brainstem nuclei are associated with the mediation of ASR and PPI, whereas cortical and subcortical regions are associated with their modulation. However, it is still unclear how the modulatory units can influence PPI. In the present work, we developed a computational model of a neural circuit involved in the mediation (brainstem units) and modulation (cortical and subcortical units) of ASR and PPI. The activities of all units were modeled by the leaky-integrator formalism for neural population. The model reproduces basic features of PPI observed in experiments, such as the effects of changes in interstimulus interval, prepulse intensity, and habituation of ASR. The simulation of GABAergic and dopaminergic drugs impaired PPI by their effects over subcortical units activity. The results show that subcortical units constitute a central hub for PPI modulation. The presented computational model offers a valuable tool to investigate the neurobiology associated with disorder-related impairments in PPI. Full article
12 pages, 3422 KiB  
Article
Tuning Structure and Properties of a Ferromagnetic Organic Semiconductor via a Magnetic Field-Modified Reduction Process
by Han Zhou, Zaitian Cheng, Zhiqiang Ai, Xinyao Li, Lin Hu and Fapei Zhang
Magnetochemistry 2024, 10(5), 34; https://doi.org/10.3390/magnetochemistry10050034 (registering DOI) - 15 May 2024
Abstract
The development of novel synthesis and assembly strategies is critical to achieving a ferromagnetic organic semiconductor with high Curie temperature. In this study, we report a high magnetic field (HMF)-modified solvothermal approach for the reduction in neutral perylene diimide (PDI) into the dianion [...] Read more.
The development of novel synthesis and assembly strategies is critical to achieving a ferromagnetic organic semiconductor with high Curie temperature. In this study, we report a high magnetic field (HMF)-modified solvothermal approach for the reduction in neutral perylene diimide (PDI) into the dianion species to prepare the PDI magnets comprising radical anions after subsequent oxidation processes. The PDI materials, assembled from the dianion solution by an HMF-modified reduction, exhibit a smaller crystallite size and an enlarged distance of the π-π stacking in the PDI aggregates. Furthermore, the PDI magnets obtained from the process under a 9T field reveal weakened ferromagnetism and the rapid degradation of electrical conductivity compared to those prepared without a magnetic field. Based on spectral and structural characterizations, such performance deterioration originates from the enhanced instability of the radical anions exposed to air, as well as the decreased crystallinity for the radical PDIs synthesized from the HMF-modified reduction process. This work demonstrates that magnetic fields offer an effective way in the material synthesis process to manipulate the structure and magnetic properties of the radical-based organic magnets. Full article
(This article belongs to the Special Issue Recent Progress of Magnetic Field Effect on Catalysts)
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18 pages, 3559 KiB  
Article
Novel Metric for Non-Invasive Beat-to-Beat Blood Pressure Measurements Demonstrates Physiological Blood Pressure Fluctuations during Pregnancy
by David Zimmermann, Hagen Malberg and Martin Schmidt
Sensors 2024, 24(10), 3151; https://doi.org/10.3390/s24103151 (registering DOI) - 15 May 2024
Abstract
Beat-to-beat (B2B) variability in biomedical signals has been shown to have high diagnostic power in the treatment of various cardiovascular and autonomic disorders. In recent years, new techniques and devices have been developed to enable non-invasive blood pressure (BP) measurements. In this work, [...] Read more.
Beat-to-beat (B2B) variability in biomedical signals has been shown to have high diagnostic power in the treatment of various cardiovascular and autonomic disorders. In recent years, new techniques and devices have been developed to enable non-invasive blood pressure (BP) measurements. In this work, we aim to establish the concept of two-dimensional signal warping, an approved method from ECG signal processing, for non-invasive continuous BP signals. To this end, we introduce a novel BP-specific beat annotation algorithm and a B2B-BP fluctuation (B2B-BPF) metric novel for BP measurements that considers the entire BP waveform. In addition to careful validation with synthetic data, we applied the generated analysis pipeline to non-invasive continuous BP signals of 44 healthy pregnant women (30.9 ± 5.7 years) between the 21st and 30th week of gestation (WOG). In line with established variability metrics, a significant increase (p < 0.05) in B2B-BPF can be observed with advancing WOGs. Our processing pipeline enables robust extraction of B2B-BPF, demonstrates the influence of various factors such as increasing WOG or exercise on blood pressure during pregnancy, and indicates the potential of novel non-invasive biosignal sensing techniques in diagnostics. The results represent B2B-BP changes in healthy pregnant women and allow for future comparison with those signals acquired from women with hypertensive disorders. Full article
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14 pages, 743 KiB  
Perspective
An Overview of Tools and Challenges for Safety Evaluation and Exposure Assessment in Industry 4.0
by Spyridon Damilos, Stratos Saliakas, Dimitris Karasavvas and Elias P. Koumoulos
Appl. Sci. 2024, 14(10), 4207; https://doi.org/10.3390/app14104207 (registering DOI) - 15 May 2024
Abstract
Airborne pollutants pose a significant threat in the occupational workplace resulting in adverse health effects. Within the Industry 4.0 environment, new systems and technologies have been investigated for risk management and as health and safety smart tools. The use of predictive algorithms via [...] Read more.
Airborne pollutants pose a significant threat in the occupational workplace resulting in adverse health effects. Within the Industry 4.0 environment, new systems and technologies have been investigated for risk management and as health and safety smart tools. The use of predictive algorithms via artificial intelligence (AI) and machine learning (ML) tools, real-time data exchange via the Internet of Things (IoT), cloud computing, and digital twin (DT) simulation provide innovative solutions for accident prevention and risk mitigation. Additionally, the use of smart sensors, wearable devices and virtual (VR) and augmented reality (AR) platforms can support the training of employees in safety practices and signal the alarming concentrations of airborne hazards, providing support in designing safety strategies and hazard control options. Current reviews outline the drawbacks and challenges of these technologies, including the elevated stress levels of employees, cyber-security, data handling, and privacy concerns, while highlighting limitations. Future research should focus on the ethics, policies, and regulatory aspects of these technologies. This perspective puts together the advances and challenges of Industry 4.0 innovations in terms of occupational safety and exposure assessment, aiding in understanding the full potential of these technologies and supporting their application in industrial manufacturing environments. Full article
(This article belongs to the Special Issue The Future of Manufacturing and Industry 4.0)
29 pages, 544 KiB  
Systematic Review
The Impact of Role Models and Mentors on the Mental and Physical Wellbeing of Sexual and Gender Minorities
by Jason Cottle, Anna L. Drozdik and Katharine A. Rimes
Behav. Sci. 2024, 14(5), 417; https://doi.org/10.3390/bs14050417 (registering DOI) - 15 May 2024
Abstract
Sexual and gender minorities (SGMs) experience a higher mental health burden compared to their cisgender, heterosexual counterparts. Role models and mentors are important for wellbeing and development; however, little evidence exists exploring their impact on SGM people. This systematic scoping review identifies their [...] Read more.
Sexual and gender minorities (SGMs) experience a higher mental health burden compared to their cisgender, heterosexual counterparts. Role models and mentors are important for wellbeing and development; however, little evidence exists exploring their impact on SGM people. This systematic scoping review identifies their association with mental and physical wellbeing. Eight databases (Medline, Embase, Cochrane CENTRAL, ERIC, Science Citation Index, Scopus, EPub and PsychInfo) were searched for eligible publications from 2000 to 2022. Two researchers identified studies, extracted data, completed quality appraisals using CASP checklists, and grouped data into outcomes relating to role model impact. From 501 citations, 12 studies (n = 1468 SGM people aged 15–63 years) were included. Positive role models and mentors encouraged identity acceptance through destigmatisation and positive affirmation, increased SGMs’ psychological wellbeing through improved psychological safety and self-confidence and improved their sexual health knowledge. Potential role models and mentors displaying negative behaviours could cause stigmatisation, as well as reduce identity acceptance and psychological safety. Information regarding the perceived influence of role models and mentors on substance abuse and other physical health outcomes was limited. SGMs report greater benefits from relationships with others of shared minority status, providing incentives to match mentees with role models and mentors who share or empathise with their experiences of marginalization. Full article
(This article belongs to the Special Issue Contemporary Perspectives on LGBTQ Psychology and Health)
17 pages, 712 KiB  
Article
Effects of Sustainable Rice Management on the Behavior and Bioefficacy of Bispyribac-Sodium: A Medium-Term Study
by Antonio López-Piñeiro, Luis Vicente, Damián Fernández-Rodríguez, Ángel Albarrán, José Manuel Rato Nunes and David Peña
Sustainability 2024, 16(10), 4157; https://doi.org/10.3390/su16104157 (registering DOI) - 15 May 2024
Abstract
The practices (tillage and flooding) used for rice crops are unsustainable, especially in areas characterized by water shortages, such as the Mediterranean region. Therefore, it is necessary to develop sustainable methods in order to ensure the viability of rice production. However, it is [...] Read more.
The practices (tillage and flooding) used for rice crops are unsustainable, especially in areas characterized by water shortages, such as the Mediterranean region. Therefore, it is necessary to develop sustainable methods in order to ensure the viability of rice production. However, it is essential to understand the effects that alternative management can have on herbicide behavior. In this context, this paper describes the first field experiment conducted to evaluate the medium-term effects of different agricultural practices on the fate of bispyribac sodium (BPS). Thus, the treatments were as follows: tillage and flooding (TF), tillage and sprinkler (TS), and no-tillage and sprinkler (NTS). In addition, “alperujo” compost (AC) from olive mill waste was used in each treatment (TF-AC, TS-AC, and NTS-AC). The AC was applied only once in 2015 when the TS and NTS treatments were implemented. The AC amendment increased the adsorption of BPS and its irreversibility, thereby decreasing the BPS leaching capacity. Furthermore, throughout this study, the BPS persistence was up to 1.85 times greater with sprinklers than in the flooding condition, which could explain the high values of BPS effectiveness (increased by a factor of 1.45 on average) found with sprinklers. Therefore, the implementation of sprinklers in combination with AC can be considered a sustainable strategy for Mediterranean rice production, at least in the medium term, as it reduces BPS water pollution and increases its weed control efficiency. Full article
(This article belongs to the Section Sustainable Agriculture)
11 pages, 401 KiB  
Article
Fuzzy Classification Approach to Select Learning Objects Based on Learning Styles in Intelligent E-Learning Systems
by Ibtissam Azzi, Abdelhay Radouane, Loubna Laaouina, Adil Jeghal, Ali Yahyaouy and Hamid Tairi
Informatics 2024, 11(2), 29; https://doi.org/10.3390/informatics11020029 (registering DOI) - 15 May 2024
Abstract
In e-learning systems, even though the automatic detection of learning styles is considered the key element in the adaptation process, it does not represent the main goal of this process at all. Indeed, to accomplish the task of adaptation, it is also necessary [...] Read more.
In e-learning systems, even though the automatic detection of learning styles is considered the key element in the adaptation process, it does not represent the main goal of this process at all. Indeed, to accomplish the task of adaptation, it is also necessary to be able to automatically select the learning objects according to the detected styles. The classification techniques are the most used techniques to automatically select the learning objects by processing data derived from learning object metadata. By using these classification techniques, considerable results are obtained via several approaches and consist of mapping the learning objects into different teaching strategies and then mapping these strategies into the identified learning styles. However, these approaches have some limitations related to robustness. Indeed, a common feature of these approaches is that they do not directly map learning object metadata elements to learning style dimensions. Moreover, they do not consider the fuzzy nature of learning objects. Indeed, any learning object can be suitable for different learning styles at varying degrees of suitability. This highlights the need to find a way to remedy this shortcoming. Our work is part of the automatic selection of learning objects. So, we will propose an approach that uses the fuzzy classification technique to select learning objects based on learning styles. In this approach, the metadata of each learning object that complies with the Institute of Electrical and Electronics Engineers (IEEE) standard are stored in a database as an Extensible Markup Language (XML) file. The Fuzzy C Means algorithm is used, on one hand, to assign fuzzy suitability rates to the stored learning objects and, on the other hand, to cluster them into the Felder and Silverman learning styles model categories. The experiment results show the performance of our approach. Full article
17 pages, 2659 KiB  
Article
Modulation of Gut Microbial Community and Metabolism by Bacillus licheniformis HD173 Promotes the Growth of Nursery Piglets Model
by Jiaxuan Li, Cheng Tian, Shuaifei Feng, Wei Cheng, Shiyu Tao, Changchun Li, Yuncai Xiao and Hong Wei
Nutrients 2024, 16(10), 1497; https://doi.org/10.3390/nu16101497 (registering DOI) - 15 May 2024
Abstract
Maintaining the balance and stability of the gut microbiota is crucial for the gut health and growth development of humans and animals. Bacillus licheniformis (B. licheniformis) has been reported to be beneficial to the gut health of humans and [...] Read more.
Maintaining the balance and stability of the gut microbiota is crucial for the gut health and growth development of humans and animals. Bacillus licheniformis (B. licheniformis) has been reported to be beneficial to the gut health of humans and animals, whereas the probiotic effects of a new strain, B. licheniformis HD173, remain uncertain. In this study, nursery piglets were utilized as animal models to investigate the extensive impact of B. licheniformis HD173 on gut microbiota, metabolites, and host health. The major findings were that this probiotic enhanced the growth performance and improved the health status of the nursery piglets. Specifically, it reduced the level of pro-inflammatory cytokines IL-1β and TNF-α in the serum while increasing the level of IL-10 and SOD. In the gut, B. licheniformis HD173 reduced the abundance of pathogenic bacteria such as Mycoplasma, Vibrio, and Vibrio metschnikovii, while it increased the abundance of butyrate-producing bacteria, including Oscillospira, Coprococcus, and Roseburia faecis, leading to an enhanced production of butyric acid. Furthermore, B. licheniformis HD173 effectively improved the gut metabolic status, enabling the gut microbiota to provide the host with stronger metabolic abilities for nutrients. In summary, these findings provide scientific evidence for the utilization of B. licheniformis HD173 in the development and production of probiotic products for maintaining gut health in humans and animals. Full article
(This article belongs to the Section Prebiotics and Probiotics)
14 pages, 1074 KiB  
Article
Investigation of High-Speed Rubbing Behavior of GH4169 Superalloy with SiC/SiC Composites
by Zhaoguo Mi, Kanghe Jiang, Yicheng Yang, Zhenhua Cheng, Weihua Yang and Zhigang Sun
Aerospace 2024, 11(5), 397; https://doi.org/10.3390/aerospace11050397 (registering DOI) - 15 May 2024
Abstract
The silicon carbide fiber-reinforced silicon carbide matrix (SiC/SiC), ceramic matrix composite (CMC) and nickel-based superalloy GH4169 can be utilized in high-temperature applications due to their high-temperature performance. The SiC/SiC composites are commonly used in turbine outer rings, where they encounter friction and wear [...] Read more.
The silicon carbide fiber-reinforced silicon carbide matrix (SiC/SiC), ceramic matrix composite (CMC) and nickel-based superalloy GH4169 can be utilized in high-temperature applications due to their high-temperature performance. The SiC/SiC composites are commonly used in turbine outer rings, where they encounter friction and wear against the turbine blades. This high-speed rubbing occurs frequently in aircraft engines and steam turbines. To investigate the tribological behavior of these materials, rubbing experiments were conducted between the SiC/SiC and the GH4169 superalloy. The experiments involved varying the blade tip speeds ranging from 100 m/s to 350 m/s and incursion rates from 5 μm/s to 50 μm/s at room temperature. Additionally, experiments were conducted at high temperatures to compare the tribological behavior under ambient conditions. The results indicated that the GH4169 superalloy exhibited abrasive furrow wear during rubbing at both room temperature and high temperature. Furthermore, at elevated temperatures, some of the GH4169 superalloy adhered to the surface of the SiC/SiC. The analysis of the experiments conducted at ambient temperatures revealed that the friction coefficient increased with higher blade tip velocities (100~350 m/s). However, the coefficient was lower at high temperatures compared to room temperature. Furthermore, significant temperature increases were observed during rubbing at room temperature, whereas minimal temperature changes were detected on the rubbing surface at high temperatures. Full article
13 pages, 2995 KiB  
Article
Ovarian Transcriptome Analysis Reveals the Mechanism of Translucent Eggshell Formation
by Ying Ma, Yuxing Luo, Wen Li, Dehe Wang and Zhonghua Ning
Animals 2024, 14(10), 1477; https://doi.org/10.3390/ani14101477 (registering DOI) - 15 May 2024
Abstract
The presence of translucent eggshells is a type of egg quality issue that impacts egg sales. While many researchers have studied them, the exact mechanisms behind their formation remain unclear. In this study, we conducted a transcriptomic differential expression analysis of the isthmus [...] Read more.
The presence of translucent eggshells is a type of egg quality issue that impacts egg sales. While many researchers have studied them, the exact mechanisms behind their formation remain unclear. In this study, we conducted a transcriptomic differential expression analysis of the isthmus region of the oviduct in both normal egg- and translucent egg-laying hens. The analysis revealed that differentially expressed gene pathways were predominantly concentrated in the synthesis, modification, and transport of eggshell membrane proteins, particularly collagen proteins, which provide structural support. These findings suggest that variations in the physical structure of the eggshell membrane, resulting from changes in its chemical composition, are the fundamental cause of translucent eggshell formation. This research provides a theoretical reference for reducing the occurrence of translucent eggs. Full article
(This article belongs to the Section Poultry)
9 pages, 1214 KiB  
Article
Iris Pigmented Lesions and Risk of Cutaneous Melanoma: Case–Control Study in Lithuania
by Lukas Šemeklis, Laura Kapitanovaitė, Grinvydas Butrimas, Kamilija Briedė, Augustė Dubinskaitė, Reda Žemaitienė and Skaidra Valiukevičienė
J. Pers. Med. 2024, 14(5), 530; https://doi.org/10.3390/jpm14050530 (registering DOI) - 15 May 2024
Abstract
The global incidence of cutaneous melanoma (CM) is rising, necessitating early detection and identification of risk factors across different populations. A case–control study with 180 patients with primary diagnosed CM and 182 healthy controls was conducted. Participants underwent ophthalmic and skin examinations, where [...] Read more.
The global incidence of cutaneous melanoma (CM) is rising, necessitating early detection and identification of risk factors across different populations. A case–control study with 180 patients with primary diagnosed CM and 182 healthy controls was conducted. Participants underwent ophthalmic and skin examinations, where the identification and counting of common melanocytic nevi (CMN) and atypical melanocytic nevi (AMN) was performed. During ophthalmic examination, high-resolution slit lamp iris images were taken. Images were categorized according to iris periphery, collaret, and freckles. There was no difference in iris periphery and collaret color between groups. However, blue/grey iris periphery and blue collaret with or without freckles were the most common patterns. The presence of pigmented iris lesions and 2–5 mm and ≥5 mm in diameter CMNs was strongly associated with CM risk. The evidence from this study indicates that blue or grey periphery and blue collaret iris pattern with iris freckles are 2.74 times higher in the CM group than controls. Further research is needed to explore iris patterns’ association with CM risk in diverse populations. Full article
(This article belongs to the Section Epidemiology)
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17 pages, 277 KiB  
Article
International Diversification and Stock-Price Crash Risk
by Alireza Askarzadeh, Mostafa Kanaanitorshizi, Maryam Tabarhosseini and Dana Amiri
Int. J. Financial Stud. 2024, 12(2), 47; https://doi.org/10.3390/ijfs12020047 (registering DOI) - 15 May 2024
Abstract
Despite the recent proliferation of research on internationalization, little attention has been paid to understanding the reasons behind the decrease in firm value accompanying international expansion. By delving into the underlying mechanisms and applying the concept of agency theory to a sample of [...] Read more.
Despite the recent proliferation of research on internationalization, little attention has been paid to understanding the reasons behind the decrease in firm value accompanying international expansion. By delving into the underlying mechanisms and applying the concept of agency theory to a sample of US firms spanning from 2000 to 2022, we posit that an increased level of information asymmetry in internationally diversified firms incentivizes managers to prioritize their own interests. To protect their careers, CEOs of internationally diversified firms often suppress bad news. This behavior can lead to the accumulation of negative news and heighten the risk of a stock-price crash. Furthermore, we propose that higher levels of international experience, enhanced monitoring effectiveness, and efficient investment practices will negatively moderate the positive relationship between internationalization and stock-price crash risk. Full article
12 pages, 518 KiB  
Article
Safety and Efficacy of High-Dose Chemotherapy with TreoMel 200 vs. TreoMel 140 in Acute Myeloid Leukemia Patients Undergoing Autologous Stem Cell Transplantation
by Matthias Eggimann, Dilara Akhoundova, Henning Nilius, Michèle Hoffmann, Michael Hayoz, Yolanda Aebi, Carlo R. Largiadèr, Michael Daskalakis, Ulrike Bacher and Thomas Pabst
Cancers 2024, 16(10), 1887; https://doi.org/10.3390/cancers16101887 (registering DOI) - 15 May 2024
Abstract
(1) Background: Treosulfan and melphalan (TreoMel)-based high-dose chemotherapy (HDCT) has shown promising safety and efficacy as a conditioning regimen for acute myeloid leukemia (AML) patients undergoing autologous stem cell transplantation (ASCT). However, despite intensive first-line induction treatment and upfront consolidation with HDCT and [...] Read more.
(1) Background: Treosulfan and melphalan (TreoMel)-based high-dose chemotherapy (HDCT) has shown promising safety and efficacy as a conditioning regimen for acute myeloid leukemia (AML) patients undergoing autologous stem cell transplantation (ASCT). However, despite intensive first-line induction treatment and upfront consolidation with HDCT and ASCT, AML relapse rates are still high, and further efforts are needed to improve patient outcomes. The aim of this study was to compare two melphalan dose schedules in regard to the safety of TreoMel HDCT and patient outcomes. (2) Methods: We retrospectively analyzed the safety and efficacy of two melphalan dose schedules combined with standard-dose treosulfan in AML patients undergoing HDCT and ASCT at the University Hospital of Bern, Switzerland, between August 2019 and August 2023. Patients received treosulfan 42 g/m2 combined with either melphalan 140 mg/m2 (TreoMel 140) or melphalan 200 mg/m2 (TreoMel 200). Co-primary endpoints were progression-free survival (PFS), overall survival (OS), as well as safety profile. (3) Results: We included a total of 51 AML patients: 31 (60.8%) received TreoMel 140 and 20 (39.2%) TreoMel 200. The patients’ basal characteristics were comparable between both cohorts. No significant differences in the duration of hospitalization or the adverse event profile were identified. There were no statistically significant differences in relapse (0.45 vs. 0.30, p = 0.381) and mortality rates (0.42 vs. 0.15, p = 0.064) between the melphalan 140 mg/m2 and 200 mg/m2 cohorts, nor for PFS (HR: 0.81, 95% CI: 0.29–2.28, p = 0.70) or OS (HR: 0.70, 95% CI: 0.19–2.57, p = 0.59) for the TreoMel 140 vs. TreoMel 200 cohort. (4) Conclusions: A higher dose of melphalan (TreoMel 200) was well tolerated overall. No statistically significant differences for patient outcomes could be observed, possibly due to the relatively small patient cohort and the short follow-up. A longer follow-up and prospective randomized studies would be required to confirm the safety profile and clinical benefit. Full article
(This article belongs to the Special Issue 2nd Edition of New Therapeutic Strategies for Acute Myeloid Leukemia)
50 pages, 652 KiB  
Article
Non-Negative Decomposition of Multivariate Information: From Minimum to Blackwell-Specific Information
by Tobias Mages, Elli Anastasiadi and Christian Rohner
Entropy 2024, 26(5), 424; https://doi.org/10.3390/e26050424 (registering DOI) - 15 May 2024
Abstract
Partial information decompositions (PIDs) aim to categorize how a set of source variables provides information about a target variable redundantly, uniquely, or synergetically. The original proposal for such an analysis used a lattice-based approach and gained significant attention. However, finding a suitable underlying [...] Read more.
Partial information decompositions (PIDs) aim to categorize how a set of source variables provides information about a target variable redundantly, uniquely, or synergetically. The original proposal for such an analysis used a lattice-based approach and gained significant attention. However, finding a suitable underlying decomposition measure is still an open research question at an arbitrary number of discrete random variables. This work proposes a solution with a non-negative PID that satisfies an inclusion–exclusion relation for any f-information measure. The decomposition is constructed from a pointwise perspective of the target variable to take advantage of the equivalence between the Blackwell and zonogon order in this setting. Zonogons are the Neyman–Pearson region for an indicator variable of each target state, and f-information is the expected value of quantifying its boundary. We prove that the proposed decomposition satisfies the desired axioms and guarantees non-negative partial information results. Moreover, we demonstrate how the obtained decomposition can be transformed between different decomposition lattices and that it directly provides a non-negative decomposition of Rényi-information at a transformed inclusion–exclusion relation. Finally, we highlight that the decomposition behaves differently depending on the information measure used and how it can be used for tracing partial information flows through Markov chains. Full article
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8 pages, 837 KiB  
Communication
Enhanced Copper Bonding Interfaces by Quenching to Form Wrinkled Surfaces
by Tsan-Feng Lu, Yu-Ting Yen, Pei-Wen Wang, Yuan-Fu Cheng, Cheng-Hsiang Chen and YewChung Sermon Wu
Nanomaterials 2024, 14(10), 861; https://doi.org/10.3390/nano14100861 (registering DOI) - 15 May 2024
Abstract
For decades, Moore’s Law has been approaching its limits, posing a huge challenge for further downsizing to nanometer dimensions. A promising avenue to replace Moore’s Law lies in three-dimensional integrated circuits, where Cu–Cu bonding plays a critical role. However, the atomic diffusion rate [...] Read more.
For decades, Moore’s Law has been approaching its limits, posing a huge challenge for further downsizing to nanometer dimensions. A promising avenue to replace Moore’s Law lies in three-dimensional integrated circuits, where Cu–Cu bonding plays a critical role. However, the atomic diffusion rate is notably low at temperatures below 300 °C, resulting in a distinct weak bonding interface, which leads to reliability issues. In this study, a quenching treatment of the Cu film surface was investigated. During the quenching treatment, strain energy was induced due to the variation in thermal expansion coefficients between the Si substrate and the Cu film, resulting in a wrinkled surface morphology on the Cu film. Grain growth was observed at the Cu–Cu bonding interface following bonding at 300 °C for 2 and 4 h. Remarkably, these procedures effectively eliminated the bonding interface. Full article
(This article belongs to the Special Issue Nano Surface Engineering)
13 pages, 955 KiB  
Article
Eminent Antimicrobial Peptide Resistance in Zymomonas mobilis: A Novel Advantage of Intrinsically Uncoupled Energetics
by Reinis Rutkis, Zane Lasa, Marta Rubina, Inese Strazdina and Uldis Kalnenieks
Antibiotics 2024, 13(5), 451; https://doi.org/10.3390/antibiotics13050451 (registering DOI) - 15 May 2024
Abstract
Relative to several model bacteria, the ethanologenic bacterium Zymomonas mobilis is shown here to have elevated resistance to exogenous antimicrobial peptides (AMPs)—with regard to both peptide bulk concentration in the medium and the numbers of peptide molecules per cell. By monitoring the integration [...] Read more.
Relative to several model bacteria, the ethanologenic bacterium Zymomonas mobilis is shown here to have elevated resistance to exogenous antimicrobial peptides (AMPs)—with regard to both peptide bulk concentration in the medium and the numbers of peptide molecules per cell. By monitoring the integration of AMPs in the bacterial cell membrane and observing the resulting effect on membrane energy coupling, it is concluded that the membranotropic effects of the tested AMPs in Z. mobilis and in Escherichia coli are comparable. The advantage of Z. mobilis over E. coli apparently results from its uncoupled mode of energy metabolism that, in contrast to E. coli, does not rely on oxidative phosphorylation, and hence, is less vulnerable to the disruption of its energy-coupling membrane by AMPs. It is concluded that the high resistance to antimicrobial peptides (AMPs) observed in Z. mobilis not only proves crucial for its survival in its natural environment but also offers a promising platform for AMP production and sheds light on potential strategies for novel resistance development in clinical settings. Full article
(This article belongs to the Section Antimicrobial Peptides)
18 pages, 2092 KiB  
Article
Paralytic Shellfish Toxins in Mollusks from Galicia Analyzed by a Fast Refined AOAC 2005.06 Method: Toxicity, Toxin Profile, and Inter-Specific, Spatial, and Seasonal Variations
by Juan Blanco, Juan Pablo Lamas, Fabiola Arévalo, Jorge Correa, Tamara Rodríguez-Cabo and Ángeles Moroño
Toxins 2024, 16(5), 230; https://doi.org/10.3390/toxins16050230 (registering DOI) - 15 May 2024
Abstract
Paralytic shellfish poisoning is an important concern for mollusk fisheries, aquaculture, and public health. In Galicia, NW Iberian Peninsula, such toxicity has been monitored for a long time using mouse bioassay. Therefore, little information exists about the precise toxin analogues and their possible [...] Read more.
Paralytic shellfish poisoning is an important concern for mollusk fisheries, aquaculture, and public health. In Galicia, NW Iberian Peninsula, such toxicity has been monitored for a long time using mouse bioassay. Therefore, little information exists about the precise toxin analogues and their possible transformations in diverse mollusk species and environments. After the change in the European PSP reference method, a refinement of the Lawrence method was developed, achieving a 75% reduction in chromatogram run time. Since the beginning of 2021, when this refinement Lawrence method was accredited under the norm UNE-EN ISO/IEC 17025, it has been used in the area to determine the toxin profiles and to estimate PSP toxicity in more than 4500 samples. In this study, we have summarized three years of monitoring results, including interspecific, seasonal, and geographical variability of PSP toxicity and toxin profile. PSP was detected in more than half of the samples analyzed (55%), but only 4.4% of the determinations were above the EU regulatory limit. GTX1,4 was the pair of STX analogs that produced the highest toxicities, but GTX2,3 was found in most samples, mainly due to the reduction of GTX1,4 but also by the higher sensitivity of the method for this pair of analogs. STX seems to be mainly a product of biotransformation from GTX2,3. The studied species (twelve bivalves and one gastropod) accumulated and transformed PSP toxins to a different extent, with most of them showing similar profiles except for Spisula solida and Haliotis tuberculata. Two seasonal peaks of toxicity were found: one in spring-early summer and another in autumn, with slightly different toxin profiles during outbreaks in relation to the toxicity during valleys. In general, both the total toxicity and toxin profiles of the southernmost locations were different from those in the northern part of the Atlantic coast and the Cantabrian Sea, but this general pattern is modified by the PSP history of some specific locations. Full article
15 pages, 2366 KiB  
Article
Investigating the Spatial Accessibility and Coverage of the Pediatric COVID-19 Vaccine: An Ecologic Study of Regional Health Data
by Amin Bemanian and Jonathan F. Mosser
Vaccines 2024, 12(5), 545; https://doi.org/10.3390/vaccines12050545 (registering DOI) - 15 May 2024
Abstract
The COVID-19 pandemic presented the unique challenge of having to deliver novel vaccines during a public health crisis. For pediatric patients, it was further complicated by the delayed timeline for authorizing the vaccine and the differences in dosing/products depending on the patient’s age. [...] Read more.
The COVID-19 pandemic presented the unique challenge of having to deliver novel vaccines during a public health crisis. For pediatric patients, it was further complicated by the delayed timeline for authorizing the vaccine and the differences in dosing/products depending on the patient’s age. This paper investigates the relationship between the spatial accessibility and uptake of the COVID-19 vaccine in King County, WA, USA. Public data for COVID-19 vaccine sites were used to calculate spatial accessibility using an enhanced two-step floating catchment area (E2SFCA) technique. Spatial regression analyses were performed to look at the relationship between spatial accessibility and ZIP-code-level vaccination rates. The relationships of these data with other socioeconomic and demographic variables were calculated as well. Higher rates of vaccine accessibility and vaccine coverage were found in adolescent (12- to 17-year-old) individuals relative to school-age (5- to 11-year-old) individuals. Vaccine accessibility was positively associated with coverage in both age groups in the univariable analysis. This relationship was affected by neighborhood educational attainment. This paper demonstrates how measures such as E2SFCA can be used to calculate the accessibility of the COVID-19 vaccine in a region and provides insight into some of the ecological factors that affect COVID-19 vaccination rates. Full article
(This article belongs to the Section COVID-19 Vaccines and Vaccination)
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17 pages, 1665 KiB  
Article
The Biological Impact of Some Phosphonic and Phosphinic Acid Derivatives on Human Osteosarcoma
by Zakzak Khaled, Gheorghe Ilia, Claudia Watz, Ioana Macașoi, George Drăghici, Vasile Simulescu, Petru Eugen Merghes, Narcis Ion Varan, Cristina Adriana Dehelean, Lavinia Vlaia and Laurențiu Sima
Curr. Issues Mol. Biol. 2024, 46(5), 4815-4831; https://doi.org/10.3390/cimb46050290 (registering DOI) - 15 May 2024
Abstract
Osteosarcoma malignancy currently represents a major health problem; therefore, the need for new therapy approaches is of great interest. In this regard, the current study aims to evaluate the anti-neoplastic potential of a newly developed phosphinic acid derivative (2-carboxyethylphenylphosphinic acid) and, subsequently, to [...] Read more.
Osteosarcoma malignancy currently represents a major health problem; therefore, the need for new therapy approaches is of great interest. In this regard, the current study aims to evaluate the anti-neoplastic potential of a newly developed phosphinic acid derivative (2-carboxyethylphenylphosphinic acid) and, subsequently, to outline its pharmaco-toxicological profile by employing two different in vitro human cell cultures (keratinocytes—HaCaT—and osteosarcoma SAOS-2 cells), employing different techniques (MTT assay, cell morphology assessment, LDH assay, Hoechst staining and RT-PCR). Additionally, the results obtained are compared with three commercially available phosphorus-containing compounds (P1, P2, P3). The results recorded for the newly developed compound (P4) revealed good biocompatibility (cell viability of 77%) when concentrations up to 5 mM were used on HaCaT cells for 24 h. Also, the HaCaT cultures showed no significant morphological alterations or gene modulation, thus achieving a biosafety profile even superior to some of the commercial products tested herein. Moreover, in terms of anti-osteosarcoma activity, 2-carboxyethylphenylphosphinic acid expressed promising activity on SAOS-2 monolayers, the cells showing viability of only 55%, as well as apoptosis features and important gene expression modulation, especially Bid downregulation. Therefore, the newly developed compound should be considered a promising candidate for further in vitro and in vivo research related to osteosarcoma therapy. Full article
18 pages, 1019 KiB  
Article
Evaluation of Alternative Sources of Proteins and Other Nutrients with Potential Applications in Fish Nutrition
by George-Cătălin Muntean, Dorina Simedru, Paul Uiuiu, Claudiu Tanaselia, Oana Cadar, Anca Becze and Aurelia Coroian
Molecules 2024, 29(10), 2332; https://doi.org/10.3390/molecules29102332 (registering DOI) - 15 May 2024
Abstract
The European Union’s (EU) agricultural self-sufficiency is challenged by its reliance on imported plant proteins, particularly soy from the Americas, contributing to deforestation and greenhouse gas emissions. Addressing the EU’s protein deficit, this study evaluates alternative protein sources for aquaculture, focusing on their [...] Read more.
The European Union’s (EU) agricultural self-sufficiency is challenged by its reliance on imported plant proteins, particularly soy from the Americas, contributing to deforestation and greenhouse gas emissions. Addressing the EU’s protein deficit, this study evaluates alternative protein sources for aquaculture, focusing on their nutritional value, elemental content, and polycyclic aromatic hydrocarbons (PAHs). Protein flours from gastropods (Helix pomatia, Arion lusitanicus, Arion vulgaris) and their hepatopancreas, along with plant-based proteins from food industry by-products (oilcakes, coffee grounds, spent brewer’s yeast), were analyzed. Results revealed that snail flour contained the highest protein content at 59.09%, significantly outperforming hepatopancreas flour at 42.26%. Plant-based proteins demonstrated substantial nutritional value, with coffee grounds flour exhibiting a remarkable protein content of 71.8% and spent brewer’s yeast flour at 57.9%. Elemental analysis indicated high levels of essential minerals such as magnesium in hepatopancreas flour (5719.10 mg/kg) and calcium in slug flour (48,640.11 mg/kg). However, cadmium levels in hepatopancreas flour (11.45 mg/kg) necessitate caution due to potential health risks. PAH concentrations were low across all samples, with the highest total PAH content observed in hepatopancreas flour at 0.0353 µg/kg, suggesting minimal risk of PAH-related toxicity. The analysis of plant-based protein sources, particularly oilcakes derived from sunflower, hemp, flax, and pumpkin seeds, revealed that these by-products not only exhibit high protein contents but present a promising avenue for enhancing the nutritional quality of feed. This study underscores the potential of utilizing gastropod and plant-based by-products as sustainable and nutritionally adequate alternatives to conventional feeds in aquaculture, contributing to the EU’s environmental sustainability goals. Full article
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11 pages, 2358 KiB  
Review
Humidification during Invasive and Non-Invasive Ventilation: A Starting Tool Kit for Correct Setting
by Riccardo Re, Sergio Lassola, Silvia De Rosa and Giacomo Bellani
Med. Sci. 2024, 12(2), 26; https://doi.org/10.3390/medsci12020026 (registering DOI) - 15 May 2024
Abstract
The humidification process of medical gases plays a crucial role in both invasive and non-invasive ventilation, aiming to mitigate the complications arising from bronchial dryness. While passive humidification systems (HME) and active humidification systems are prevalent in routine clinical practice, there is a [...] Read more.
The humidification process of medical gases plays a crucial role in both invasive and non-invasive ventilation, aiming to mitigate the complications arising from bronchial dryness. While passive humidification systems (HME) and active humidification systems are prevalent in routine clinical practice, there is a pressing need for further evaluation of their significance. Additionally, there is often an incomplete understanding of the operational mechanisms of these devices. The current review explores the historical evolution of gas conditioning in clinical practice, from early prototypes to contemporary active and passive humidification systems. It also discusses the physiological principles underlying humidity regulation and provides practical guidance for optimizing humidification parameters in both invasive and non-invasive ventilation modalities. The aim of this review is to elucidate the intricate interplay between temperature, humidity, and patient comfort, emphasizing the importance of individualized approaches to gas conditioning. Full article
(This article belongs to the Section Critical Care Medicine)
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18 pages, 1347 KiB  
Article
Design and Test of Automatic Feeding Device for Substrate Filling
by Haibing Pan, Qinchao Xu, Xiulan Bao, Ran Zhou, Jian Zhang, Cheng Shan and Shanjun Li
Appl. Sci. 2024, 14(10), 4206; https://doi.org/10.3390/app14104206 (registering DOI) - 15 May 2024
Abstract
An automatic feeding device for substrate filling was designed to address the challenges of difficult feeding and low efficiency in the citrus seedling pot filling and transferring machine. The device comprises a framework, tracks, overturning frame, drive system, etc. In order to ensure [...] Read more.
An automatic feeding device for substrate filling was designed to address the challenges of difficult feeding and low efficiency in the citrus seedling pot filling and transferring machine. The device comprises a framework, tracks, overturning frame, drive system, etc. In order to ensure optimal performance, the frame’s turning angle was set at a minimum of 110° and the angle between the frame’s horizontal plane and slope was determined to be 120°. Following optimization, the number and intensity of sudden changes in velocity, angular velocity, and thrust were reduced, thereby prolonging the device’s service life. The prototype test demonstrated that the device has an average feeding time of 9.86 s, is capable of raising 0.14 m3 of substrate in a single cycle, and has a handcart turning angle of 111°. Furthermore, no residual substrate remained in the handcart, and the handcart fixing mechanism operated correctly. The torque measurement results of the motor output shaft were found to be consistent with the simulation results in ADAMS, with a maximum force difference of only 298 N. The simulation was found to be accurate, with an error rate of only 3.67%. This model can be utilized as a dependable reference for the optimization of the design of the automatic feeding device. Full article
15 pages, 4331 KiB  
Article
Microstructural Characterization of AlCrCuFeMnNi Complex Concentrated Alloy Prepared by Pressureless Sintering
by Tiago Silva and Augusto Lopes
Materials 2024, 17(10), 2378; https://doi.org/10.3390/ma17102378 (registering DOI) - 15 May 2024
Abstract
A significant and increasing number of studies have been dedicated to complex concentrated alloys (CCAs) due to the improved properties that these metallic materials can exhibit. However, while most of these studies employ melting techniques, only a few explore powder metallurgy and pressureless [...] Read more.
A significant and increasing number of studies have been dedicated to complex concentrated alloys (CCAs) due to the improved properties that these metallic materials can exhibit. However, while most of these studies employ melting techniques, only a few explore powder metallurgy and pressureless sintering as production methods. In this work, a microstructural characterization of AlCrCuFeMnNi CCA samples obtained by powder metallurgy and pressureless sintering using mixtures of powders with different compositions was carried out. One batch of samples (B1) was prepared using commercial powders of Al, Cr, Cu, Fe, Mn, and Ni. Another batch (B2) used mixtures of CrFeMn, AlNi, and Cu powders. A third set of samples (B3) was obtained by adding 1% at. of Mg to the B2 powder. The samples were characterized by X-ray diffraction, scanning and transmission electron microscopy, energy dispersive spectroscopy, density measurements, and hardness tests. Thermodynamic calculations were also used to complement the microstructural characterization. All the obtained samples exhibited high relative density and hardness values. However, B3 samples showed a higher hardness, attributed to the finer distribution of oxide particles, which was promoted by the presence of Mg during sintering. These last samples presented a hardness/density ratio of 62 HV/(g cm−3), surpassing that of some martensitic stainless steels and nickel–titanium alloys. Full article
(This article belongs to the Section Metals and Alloys)

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