The 2023 MDPI Annual Report has
been released!
 
13 pages, 325 KiB  
Article
Quality of Life in Patients with Chronic Low Back Pain and Differences by Sex: A Longitudinal Study
by Xavier Pericot-Mozo, Rosa Suñer-Soler, Glòria Reig-Garcia, Josefina Patiño-Masó, Miquel Sitjar-Suñer, Afra Masià-Plana and Carme Bertran-Noguer
J. Pers. Med. 2024, 14(5), 496; https://doi.org/10.3390/jpm14050496 (registering DOI) - 08 May 2024
Abstract
Background: The experience of chronic low back pain has a significant impact on the quality of life of affected people, resulting in difficulties in performing basic activities of daily living. Aim: To study the perceived quality of life of people affected by chronic [...] Read more.
Background: The experience of chronic low back pain has a significant impact on the quality of life of affected people, resulting in difficulties in performing basic activities of daily living. Aim: To study the perceived quality of life of people affected by chronic low back pain and the associated factors by sex. Methods: A prospective, longitudinal and observational design was used. Results: A total of 129 people (58.1% women) with chronic low back pain were studied. The mean pain intensity scores were of moderate severity (6.42 points), with a modest improvement at follow-up (6.17 points). Epidural nerve blocks were the most effective therapeutic intervention in reducing the intensity of pain. Participants described a negative perception of their health with regard to quality of life, with low scores for the two constructs both at baseline (health index, 0.444; perception of health, 38.76 points) and at follow-up (health index, 0.447; perception of health, 40.43 points). Participants had severe functional limitation scores (50.79 points). The results were significantly better among men. There was an inverse relationship between the average pain intensity (β = −0.304; p < 0.001), functional limitation (β = −0.466; p < 0.001) and mental health (β = −0.565; p < 0.001) and quality of life. Conclusions: The chronification of low back pain complicates people’s biopsychosocial adaptation to life. There is a longitudinal inverse association between pain and functional limitation and health-related quality of life. Full article
(This article belongs to the Special Issue How to Undertake Personalized Assessments and Cures for Pain)
12 pages, 1486 KiB  
Article
Assessment of the Quality and Readability of Information Provided by ChatGPT in Relation to the Use of Platelet-Rich Plasma Therapy for Osteoarthritis
by Stephen Fahy, Marcel Niemann, Peter Böhm, Tobias Winkler and Stephan Oehme
J. Pers. Med. 2024, 14(5), 495; https://doi.org/10.3390/jpm14050495 (registering DOI) - 08 May 2024
Abstract
Objective: This study aimed to evaluate the quality and readability of information generated by ChatGPT versions 3.5 and 4 concerning platelet-rich plasma (PRP) therapy in the management of knee osteoarthritis (OA), exploring whether large language models (LLMs) could play a significant role [...] Read more.
Objective: This study aimed to evaluate the quality and readability of information generated by ChatGPT versions 3.5 and 4 concerning platelet-rich plasma (PRP) therapy in the management of knee osteoarthritis (OA), exploring whether large language models (LLMs) could play a significant role in patient education. Design: A total of 23 common patient queries regarding the role of PRP therapy in knee OA management were presented to ChatGPT versions 3.5 and 4. The quality of the responses was assessed using the DISCERN criteria, and readability was evaluated using six established assessment tools. Results: Both ChatGPT versions 3.5 and 4 produced moderate quality information. The quality of information provided by ChatGPT version 4 was significantly better than version 3.5, with mean DISCERN scores of 48.74 and 44.59, respectively. Both models scored highly with respect to response relevance and had a consistent emphasis on the importance of shared decision making. However, both versions produced content significantly above the recommended 8th grade reading level for patient education materials (PEMs), with mean reading grade levels (RGLs) of 17.18 for ChatGPT version 3.5 and 16.36 for ChatGPT version 4, indicating a potential barrier to their utility in patient education. Conclusions: While ChatGPT versions 3.5 and 4 both demonstrated the capability to generate information of moderate quality regarding the role of PRP therapy for knee OA, the readability of the content remains a significant barrier to widespread usage, exceeding the recommended reading levels for PEMs. Although ChatGPT version 4 showed improvements in quality and source citation, future iterations must focus on producing more accessible content to serve as a viable resource in patient education. Collaboration between healthcare providers, patient organizations, and AI developers is crucial to ensure the generation of high quality, peer reviewed, and easily understandable information that supports informed healthcare decisions. Full article
(This article belongs to the Special Issue Personalized Medicine in Orthopaedics, 2nd Edition)
Show Figures

Figure 1

17 pages, 4659 KiB  
Article
Green-Powered Electric Public Mobility: Integrating Urban and Interurban Routes—A Case Study Analysis
by Alessandro Franco, Giovanni Lutzemberger, Marco Giorgio Bevilacqua, Francesco Giuseppe Quilici and Matilde Vezzani
Electricity 2024, 5(2), 254-270; https://doi.org/10.3390/electricity5020013 (registering DOI) - 08 May 2024
Abstract
This article proposes a particular strategy to proceed with a progressive electrification of public transport systems in cities. Starting from a bus operation model, the possible electrification of two routes is analyzed, one urban and another extra-urban in the city of Pisa. An [...] Read more.
This article proposes a particular strategy to proceed with a progressive electrification of public transport systems in cities. Starting from a bus operation model, the possible electrification of two routes is analyzed, one urban and another extra-urban in the city of Pisa. An estimate is made of the energy uses associated with certain operating modes. The maximum level of consumption is estimated at approximately 280 kWh per day per bus for the urban route and excluding some special days, less than 215 kWh per day for the extra-urban route, for which a hybrid bus is proposed. Starting from an estimate of the daily consumption for the management of the two routes, the sizing of a photovoltaic (PV) plant distributed on some modular shelters which serves to power the same routes, is carried out. The resulting system has a power of the order of 190–200 kW. The modular solution is also outlined, and an installation is proposed. The analyzed case lends itself to being easily replicated. Full article
(This article belongs to the Topic Integration of Renewable Energy)
Show Figures

Figure 1

17 pages, 2006 KiB  
Article
Does Active or Informative Messaging Result in Greater Conservation Engagement?
by Lily T. Maynard, Jennifer R. Torchalski, Zachariah J. Gezon, Karlisa A. Callwood, M. Andrew Stamper, Mandi W. Schook and Claire Martin
Conservation 2024, 4(2), 236-252; https://doi.org/10.3390/conservation4020016 (registering DOI) - 08 May 2024
Abstract
Strategic communication can motivate target audiences to take conservation action. Yet, whether audiences are motivated by more information or more influential visuals is unclear. Using online surveys, we compared different visual communication strategies using text, graphics, and photographs for encouraging parrotfish-friendly conservation behaviors [...] Read more.
Strategic communication can motivate target audiences to take conservation action. Yet, whether audiences are motivated by more information or more influential visuals is unclear. Using online surveys, we compared different visual communication strategies using text, graphics, and photographs for encouraging parrotfish-friendly conservation behaviors to see which one yields greater emotion, interest, and intended action. Experiment 1 explored whether a scientific-oriented poster would be more or less effective in promoting conservation behaviors than a graphical poster using social marketing techniques. Experiment 2 contrasted the two posters with a photograph and graphic icons without text against a blank control. Results revealed how engaging visuals can inspire reactions and behavioral intentions. The posters both elicited positive reactions, but with less text and content to process, the social marketing poster more efficiently inspired the desired reactions. This work highlights that conservation communications can strategically use psychology and graphics to efficiently inspire desired actions. Full article
Show Figures

Figure 1

10 pages, 1658 KiB  
Article
Intelligent Ship Scheduling and Path Planning Method for Maritime Emergency Rescue
by Wen Ying, Zhaohui Wang, Hui Li, Sheng Du and Man Zhao
Algorithms 2024, 17(5), 197; https://doi.org/10.3390/a17050197 (registering DOI) - 08 May 2024
Abstract
Intelligent ship navigation scheduling and planning is of great significance for ensuring the safety of maritime production and life and promoting the development of the marine economy. In this paper, an intelligent ship scheduling and path planning method is proposed for a practical [...] Read more.
Intelligent ship navigation scheduling and planning is of great significance for ensuring the safety of maritime production and life and promoting the development of the marine economy. In this paper, an intelligent ship scheduling and path planning method is proposed for a practical application scenario wherein the emergency rescue center receives rescue messages and dispatches emergency rescue ships to the incident area for rescue. Firstly, the large-scale sailing route of the task ship is pre-planned in the voyage planning stage by using the improved A* algorithm. Secondly, the full-coverage path planning algorithm is used to plan the ship’s search route in the regional search stage by updating the ship’s navigation route in real time. In order to verify the effectiveness of the proposed algorithm, comparative experiments were carried out with the conventional algorithm in the two operation stages of rushing to the incident sea area and regional search and rescue. The experimental results show that the proposed algorithm can adapt to emergency search and rescue tasks in the complex setting of the sea area and can effectively improve the efficiency of the operation, ensure the safety of the operation process, and provide a more intelligent and efficient solution for the planning of maritime emergency rescue tasks. Full article
Show Figures

Figure 1

13 pages, 9090 KiB  
Article
Discriminating Glioblastoma from Normal Brain Tissue In Vivo Using Optical Coherence Tomography and Angiography: A Texture and Microvascular Analysis Approach
by Trung Nguyễn-Hoàng, Tai-Ang Wang, Chia-Heng Wu and Meng-Tsan Tsai
Photonics 2024, 11(5), 435; https://doi.org/10.3390/photonics11050435 (registering DOI) - 08 May 2024
Abstract
Brain tumors arise from abnormal cell growth in the brain. Glioblastoma, the most common and aggressive type, poses significant challenges for identification during surgery. The primary goal of this study is to identify and differentiate normal brain tissue from glioblastoma tissue using optical [...] Read more.
Brain tumors arise from abnormal cell growth in the brain. Glioblastoma, the most common and aggressive type, poses significant challenges for identification during surgery. The primary goal of this study is to identify and differentiate normal brain tissue from glioblastoma tissue using optical coherence tomography (OCT) and OCT angiography (OCTA). These techniques offer a non-invasive way to analyze the morphological and microvascular alternations associated with glioblastoma in an animal model. To monitor the changes in morphology and vascular distribution of brain tissue as glioblastoma tumors grow, time-series OCT and OCTA results were collected for comparison. Texture analysis of OCT images was proposed using the gray-level co-occurrence matrix (GLCM), from which homogeneity and variance were calculated as discriminative parameters. Additionally, OCTA was used to assess microvascular characteristics, including vessel diameter, density, and fractal dimension. The findings demonstrate that the proposed methods can effectively distinguish between normal and cancerous brain tissue in vivo. Full article
(This article belongs to the Special Issue Photonics: 10th Anniversary)
Show Figures

Figure 1

16 pages, 4577 KiB  
Article
Human versus Artificial Intelligence: Validation of a Deep Learning Model for Retinal Layer and Fluid Segmentation in Optical Coherence Tomography Images from Patients with Age-Related Macular Degeneration
by Mariana Miranda, Joana Santos-Oliveira, Ana Maria Mendonça, Vânia Sousa, Tânia Melo and Ângela Carneiro
Diagnostics 2024, 14(10), 975; https://doi.org/10.3390/diagnostics14100975 (registering DOI) - 08 May 2024
Abstract
Artificial intelligence (AI) models have received considerable attention in recent years for their ability to identify optical coherence tomography (OCT) biomarkers with clinical diagnostic potential and predict disease progression. This study aims to externally validate a deep learning (DL) algorithm by comparing its [...] Read more.
Artificial intelligence (AI) models have received considerable attention in recent years for their ability to identify optical coherence tomography (OCT) biomarkers with clinical diagnostic potential and predict disease progression. This study aims to externally validate a deep learning (DL) algorithm by comparing its segmentation of retinal layers and fluid with a gold-standard method for manually adjusting the automatic segmentation of the Heidelberg Spectralis HRA + OCT software Version 6.16.8.0. A total of sixty OCT images of healthy subjects and patients with intermediate and exudative age-related macular degeneration (AMD) were included. A quantitative analysis of the retinal thickness and fluid area was performed, and the discrepancy between these methods was investigated. The results showed a moderate-to-strong correlation between the metrics extracted by both software types, in all the groups, and an overall near-perfect area overlap was observed, except for in the inner segment ellipsoid (ISE) layer. The DL system detected a significant difference in the outer retinal thickness across disease stages and accurately identified fluid in exudative cases. In more diseased eyes, there was significantly more disagreement between these methods. This DL system appears to be a reliable method for accessing important OCT biomarkers in AMD. However, further accuracy testing should be conducted to confirm its validity in real-world settings to ultimately aid ophthalmologists in OCT imaging management and guide timely treatment approaches. Full article
(This article belongs to the Special Issue Artificial Intelligence in Eye Disease—3rd Edition)
Show Figures

Figure 1

14 pages, 1756 KiB  
Article
Diagnostic and Prognostic Value of Plasma GFAP in Sporadic Creutzfeldt–Jakob Disease in the Clinical Setting of Rapidly Progressive Dementia
by Giuseppe Mario Bentivenga, Simone Baiardi, Andrea Mastrangelo, Corrado Zenesini, Angela Mammana, Marcello Rossi, Barbara Polischi, Sabina Capellari and Piero Parchi
Int. J. Mol. Sci. 2024, 25(10), 5106; https://doi.org/10.3390/ijms25105106 (registering DOI) - 08 May 2024
Abstract
The diagnostic and prognostic value of plasma glial fibrillary acidic protein (pl-GFAP) in sporadic Creutzfeldt–Jakob disease (sCJD) has never been assessed in the clinical setting of rapidly progressive dementia (RPD). Using commercially available immunoassays, we assayed the plasma levels of GFAP, tau (pl-tau), [...] Read more.
The diagnostic and prognostic value of plasma glial fibrillary acidic protein (pl-GFAP) in sporadic Creutzfeldt–Jakob disease (sCJD) has never been assessed in the clinical setting of rapidly progressive dementia (RPD). Using commercially available immunoassays, we assayed the plasma levels of GFAP, tau (pl-tau), and neurofilament light chain (pl-NfL) and the CSF total tau (t-tau), 14-3-3, NfL, phospho-tau181 (p-tau), and amyloid-beta isoforms 42 (Aβ42) and 40 (Aβ40) in sCJD (n = 132) and non-prion RPD (np-RPD) (n = 94) patients, and healthy controls (HC) (n = 54). We also measured the CSF GFAP in 67 sCJD patients. Pl-GFAP was significantly elevated in the sCJD compared to the np-RPD and HC groups and affected by the sCJD subtype. Its diagnostic accuracy (area under the curve (AUC) 0.760) in discriminating sCJD from np-RPD was higher than the plasma and CSF NfL (AUCs of 0.596 and 0.663) but inferior to the 14-3-3, t-tau, and pl-tau (AUCs of 0.875, 0.918, and 0.805). Pl-GFAP showed no association with sCJD survival after adjusting for known prognostic factors. Additionally, pl-GFAP levels were associated with 14-3-3, pl-tau, and pl-NfL but not with CSF GFAP, Aβ42/Aβ40, and p-tau. The diagnostic and prognostic value of pl-GFAP is inferior to established neurodegeneration biomarkers. Nonetheless, pl-GFAP noninvasively detects neuroinflammation and neurodegeneration in sCJD, warranting potential applications in disease monitoring. Full article
Show Figures

Figure 1

13 pages, 10814 KiB  
Article
Dental Implants with External Hex Inclined Shoulder in Full-Arch Immediate Loading Rehabilitations of the Maxilla
by Francesco Bagnasco, Paolo Pesce, Domenico Baldi, Francesco Motta, Francesco Pera, Nicola De Angelis and Maria Menini
Dent. J. 2024, 12(5), 131; https://doi.org/10.3390/dj12050131 (registering DOI) - 08 May 2024
Abstract
Background: Coaxial implants with an inclined neck might overcome some problems related to angulation of the implant axis when using tilted implants. Therefore, the aim of the present work was to conduct a narrative review of the current literature and to present a [...] Read more.
Background: Coaxial implants with an inclined neck might overcome some problems related to angulation of the implant axis when using tilted implants. Therefore, the aim of the present work was to conduct a narrative review of the current literature and to present a case series comparing traditional and coaxial external hex implants in full-arch immediate loading rehabilitations of the maxilla. Methods: A total of 13 external hex tapered implants (Southern Implants) was inserted in the upper jaw of 3 patients. Each patient received two tilted implants in distal sites. In one randomly selected quadrant, the tilted implant was a standard implant, while a Co-Axis® implant with a 24° inclination of the implant shoulder was inserted on the other hemi-arch. Straight conical abutments were screwed on coaxial implants while multiunit abutments of appropriate inclination were screwed as needed on the other implants to correct their axes. Peri-implant bone level was recorded radiographically at T0 (delivery of the immediate loading prosthesis), and at 3, 6, 12, and 24 months of healing and then annually. Plaque index, probing depth, and bleeding on probing were also evaluated. Cumulative implant survival rate (CSR) was calculated, and biological or technical complications were recorded as well as the operator satisfaction towards the use of coaxial implants. Results: The preliminary data collected did not show significant differences in peri-implant tissues health and maintenance over time between the two implant types. No implants failed, and both implant types proved to be favorable for full-arch rehabilitation using tilted implants. Coaxial implants facilitated the prosthodontic procedures. However, a learning curve is required in order to optimize their insertion. Conclusions: Both implants proved to be reliable and suitable for achieving clinical success in full-arch immediate loading rehabilitations, but further research with longer follow-up and larger sample size is needed to confirm these preliminary outcomes. Full article
(This article belongs to the Special Issue Advanced Research on Oral Cancer and Dental Implants)
Show Figures

Figure 1

19 pages, 4893 KiB  
Review
Effects of Multidimensional Carbon-Based Nanomaterials on the Low-Carbon and High-Performance Cementitious Composites: A Critical Review
by Xiumei Gao, Wujun Fang, Weiwen Li, Peng Wang, Kashan Khan, Yihong Tang and Teng Wang
Materials 2024, 17(10), 2196; https://doi.org/10.3390/ma17102196 (registering DOI) - 08 May 2024
Abstract
Cementitious composites are ubiquitous in construction, and more and more research is focused on improving mechanical properties and environmental effects. However, the jury is still out on which material can achieve low-carbon and high-performance cementitious composites. This article compares the mechanical and environmental [...] Read more.
Cementitious composites are ubiquitous in construction, and more and more research is focused on improving mechanical properties and environmental effects. However, the jury is still out on which material can achieve low-carbon and high-performance cementitious composites. This article compares the mechanical and environmental performance of zero-dimensional fullerenes, one-dimensional carbon nanotubes (CNTs), two-dimensional graphene oxide (GO), and three-dimensional nano-graphite platelets (NGPs) on cementitious composites. The literature review shows that two-dimensional (2D) GO has the best mechanical and environmental performance, followed by 3D NGPs, 1D CNTs, and 0D fullerenes. Specifically, GO stands out for its lower energy consumption (120–140 MJ/kg) and CO2 emissions (0.17 kg/kg). When the optimal dosage (0.01–0.05 wt%) of GO is selected, due to its high specific surface area and strong adhesion to the matrix, the compressive strength of the cementitious composites is improved by nearly 50%. This study will help engineers and researchers better utilize carbon-based nanomaterials and provide guidance and direction for future research in related fields. Full article
(This article belongs to the Section Construction and Building Materials)
Show Figures

Figure 1

15 pages, 1052 KiB  
Article
Older Adults’ Continuance Intentions for Online Physical Exercise Classes
by Francisca Taveira and Belem Barbosa
Behav. Sci. 2024, 14(5), 393; https://doi.org/10.3390/bs14050393 (registering DOI) - 08 May 2024
Abstract
During the COVID-19 pandemic, lockdowns and social distancing measures drove the shift from in-person to online physical exercise classes, leading individuals to explore these digital alternatives. Guided by the Expectation-Confirmation Model, this article examines older adults’ intentions to continue using online physical exercise [...] Read more.
During the COVID-19 pandemic, lockdowns and social distancing measures drove the shift from in-person to online physical exercise classes, leading individuals to explore these digital alternatives. Guided by the Expectation-Confirmation Model, this article examines older adults’ intentions to continue using online physical exercise classes. Semi-structured interviews were conducted with 17 adults aged 65 and older who had participated in online physical exercise classes during the pandemic. Transcripts were subject to thematic analysis using the NVivo software program. The results indicate that older adults recognize the usefulness of online physical exercise classes because of their ability to enhance their health and well-being. Their initial expectations were surpassed, and they were generally satisfied with the experience. However, in-person classes remained preferred due to their enhanced benefits. They also felt that the adoption of online classes was involuntary; instead of an autonomous decision guided by their needs and preferences, this was a viable solution imposed by the lockdown. Therefore, their continuance intentions are limited to specific conditions, namely a new lockdown or other physical impediments. Still, considering the flexibility that online physical exercise classes offer, accommodating time and physical constraints, participants highlighted the advantages of a hybrid approach for those who may face challenges attending in-person classes. Based on the findings, this article proposes that ECM provides a relevant, yet insufficient, framework for explaining older adults’ continuance intentions for online physical exercise classes, suggesting the inclusion of additional explaining factors: perceived usefulness of non-technological alternatives, necessary conditions, and self-determination. Full article
(This article belongs to the Special Issue Bridging Behavioral Sciences and Sports Sciences)
Show Figures

Figure 1

14 pages, 3678 KiB  
Article
Normalized Clinical Severity Scores Reveal a Correlation between X Chromosome Inactivation and Disease Severity in Rett Syndrome
by Jonathan K. Merritt, Xiaolan Fang, Raymond C. Caylor, Steven A. Skinner, Michael J. Friez, Alan K. Percy and Jeffrey L. Neul
Genes 2024, 15(5), 594; https://doi.org/10.3390/genes15050594 (registering DOI) - 08 May 2024
Abstract
Rett Syndrome (RTT) is a severe neurodevelopmental disorder predominately diagnosed in females and primarily caused by pathogenic variants in the X-linked gene Methyl-CpG Binding Protein 2 (MECP2). Most often, the disease causing the MECP2 allele resides on the paternal X chromosome [...] Read more.
Rett Syndrome (RTT) is a severe neurodevelopmental disorder predominately diagnosed in females and primarily caused by pathogenic variants in the X-linked gene Methyl-CpG Binding Protein 2 (MECP2). Most often, the disease causing the MECP2 allele resides on the paternal X chromosome while a healthy copy is maintained on the maternal X chromosome with inactivation (XCI), resulting in mosaic expression of one allele in each cell. Preferential inactivation of the paternal X chromosome is theorized to result in reduced disease severity; however, establishing such a correlation is complicated by known MECP2 genotype effects and an age-dependent increase in severity. To mitigate these confounding factors, we developed an age- and genotype-normalized measure of RTT severity by modeling longitudinal data collected in the US Rett Syndrome Natural History Study. This model accurately reflected individual increase in severity with age and preserved group-level genotype specific differences in severity, allowing for the creation of a normalized clinical severity score. Applying this normalized score to a RTT XCI dataset revealed that XCI influence on disease severity depends on MECP2 genotype with a correlation between XCI and severity observed only in individuals with MECP2 variants associated with increased clinical severity. This normalized measure of RTT severity provides the opportunity for future discovery of additional factors contributing to disease severity that may be masked by age and genotype effects. Full article
(This article belongs to the Section Human Genomics and Genetic Diseases)
Show Figures

Figure 1

13 pages, 1953 KiB  
Article
Screening for Antibiotic Resistance Genes in Bacteria and the Presence of Heavy Metals in the Upstream and Downstream Areas of the Wadi Hanifah Valley in Riyadh, Saudi Arabia
by Norah M. Al-Otaibi, Bassam Alsulaiman, Fahad M. Alreshoodi, Lenah E. Mukhtar, Sulaiman M. Alajel, Norah M. Binsaeedan and Fahad M. Alshabrmi
Antibiotics 2024, 13(5), 426; https://doi.org/10.3390/antibiotics13050426 (registering DOI) - 08 May 2024
Abstract
Valley surface water is considered a focal public health concern owing to the presence of multi-drug-resistant bacteria. The distribution of antimicrobial resistance (AMR) bacteria in the surface water is affected by the presence of multiple factors, including antibiotics coming from wastewater discharge or [...] Read more.
Valley surface water is considered a focal public health concern owing to the presence of multi-drug-resistant bacteria. The distribution of antimicrobial resistance (AMR) bacteria in the surface water is affected by the presence of multiple factors, including antibiotics coming from wastewater discharge or other contaminant sources such as pharmaceuticals, biocides, and heavy metals. Furthermore, there is evidence suggesting that high levels of antibiotic resistance genes (ARGs) can be transferred within bacterial communities under the influence of heavy metal stress. Hence, the primary aim of this study is to investigate the presence of heavy metals and bacterial ARGs in upstream as well as downstream locations of Wadi Hanifah Valley in Riyadh, Saudi Arabia. Sample collection was conducted at eighteen surface water sites within the valley in total. The selection of ARGs was associated with the most common antibiotics, including β-lactam, tetracycline, erythromycin, gentamicin, sulphonamide, chloramphenicol, vancomycin, trimethoprim, and colistin antibiotics, which were detected qualitatively using polymerase chain reaction (PCR) technology. The tested antibiotic resistance genes (ARGs) included (blaNDM-1 (for the antibiotic class Beta-lactamases), mecA (methicillin-resistant Staphylococcus aureus), tet(M) and tet(B) (for the antibiotic class Tetracycline), ampC (for the antibiotic class Beta-lactamases), vanA (for the antibiotic class vancomycin), mcr-1 (for the antibiotic class colistin), erm(B) (for the antibiotic class erythromycin), aac6′-Ie-aph2-Ia (for the antibiotic class Gentamicin), sulII (for the antibiotic class sulphonamide), catII (for the antibiotic class Chlorophincol), and dfrA1 (for the antibiotic class trimethoprim). Moreover, an assessment of the levels of heavy metals such as lithium (Li), beryllium (Be), chromium (Cr), cobalt (Co), arsenic (As), cadmium (Cd), tin (Sn), mercury (Hg), and lead (Pb) was conducted by using inductively coupled plasma mass spectrometry (ICPMS). According to our findings, the concentrations of sulphonamide, erythromycin, and chloramphenicol ARGs (erm(B), sulII, and catII) were observed to be the most elevated. Conversely, two ARGs, namely mecA and mcr-1, were not detected in the samples. Moreover, our data illustrated a significant rise in ARGs in the bacteria of water samples from the upstream sites as compared with the water samples from the downstream sites of Wadi Hanifah Valley. The mean concentration of Li, Be, Cr, Co, As, Cd, Sn, Hg, and Pb in the water samples was estimated to be 37.25 µg/L, 0.02 µg/L, 0.56 µg/L,0.32 µg/L, 0.93 µg/L, 0.01 µg/L, 200.4 µg/L, 0.027 µg/L, and 0.26 µg/L, respectively, for the selected 18 sites. Furthermore, it was revealed that the concentrations of the screened heavy metals in the water samples collected from various sites did not surpass the maximum limits set by the World Health Organization (WHO). In conclusion, this study offers a concise overview of the presence of heavy metals and ARGs in water samples obtained from the Wadi Hanifah Valley in Riyadh, KSA. Such findings will contribute to the ongoing monitoring and future risk assessment of ARGs spread in surface water. Full article
Show Figures

Figure 1

15 pages, 2600 KiB  
Article
Application of Opuntia ficus-indica Mucilage and Aloe Gel-Based Edible Coating to Enhance Postharvest Quality and Microbiological Aspects of Fresh Figs (Ficus carica L.)
by Giuseppe Sortino, Eugenia Guccione, Fabrizio Giuseppe Casales, Maria Lucia Valeria de Chiara, Roberta Passafiume, Alessandra Gallotta and Alessio Allegra
Horticulturae 2024, 10(5), 482; https://doi.org/10.3390/horticulturae10050482 (registering DOI) - 08 May 2024
Abstract
Fig is a widespread crop in southern Italy, highly valued for its sweet flavor. However, its consumption as a fresh product is limited to three to four days after harvest because of its high susceptibility to quality loss and microbial contamination. The combined [...] Read more.
Fig is a widespread crop in southern Italy, highly valued for its sweet flavor. However, its consumption as a fresh product is limited to three to four days after harvest because of its high susceptibility to quality loss and microbial contamination. The combined use of low temperature and a modified atmosphere is the traditional preservation method. However, several studies have shown that the use of Aloe arborescens or vera and O. ficus-indica (OFI) mucilage as an edible coating could reduce the microbial load and water loss, respectively. Therefore, our study aimed to evaluate the synergistic effects of Aloe gel (AG) and O. ficus-indica mucilage (OM) on the quality and safety of two fig cultivars, ‘San Giovanni’ and ‘Melanzana’, during cold storage at 4 °C. The main results showed the effectiveness of edible coatings on both fig cultivars. An AG coating significantly reduced the microbial load, while the OM treatment showed the ability to preserve firmness and reduce weight loss. In addition, the combined OM + AG treatment showed the same effects as the individual coating formulations, also improving visual appearance. Thus, the use of the synergetic coating formulation could be a natural way to reduce the microbial load, extending fresh fig fruit’s shelf life. Full article
(This article belongs to the Section Postharvest Biology, Quality, Safety, and Technology)
Show Figures

Figure 1

19 pages, 4457 KiB  
Article
Contributions to the Knowledge of Nemognathinae (Coleoptera: Meloidae) from China
by Shaopeng Wang, Yinuo Sun, Liang Lü and Zhao Pan
Insects 2024, 15(5), 338; https://doi.org/10.3390/insects15050338 (registering DOI) - 08 May 2024
Abstract
Despite being the most widespread blister beetle subfamily, Nemognathinae is unfairly understudied in China. In this study, a new genus and species, Sinostenoria yangi Pan, from northern China is described and illustrated. The antennae, elytra, hind wings, and claws of the new genus [...] Read more.
Despite being the most widespread blister beetle subfamily, Nemognathinae is unfairly understudied in China. In this study, a new genus and species, Sinostenoria yangi Pan, from northern China is described and illustrated. The antennae, elytra, hind wings, and claws of the new genus form a truly unique set of characteristics never observed in other genera of Nemognathini Laporte de Castelnau, 1840. Three species from China are newly recorded and illustrated: Megatrachelus sibiricus (Tauscher, 1812), Zonitomorpha dollei (Fairmaire 1889), and Stenodera djakonovi Aksentjev, 1978. The genus Oreomeloe Tan, 1981, is transferred from the tribe Meloini Gyllenhal, 1910, to Nemognathini based on an examination of the types. Aiming to test the morphology-based placement of the new genus, we conducted molecular phylogenetic analyses using two mitochondrial (COI, 16S) and three nuclear markers (28S, CAD, ITS2). The results confirm our tribal assignment of the new genus and support a clade that consists of Sinostenoriagen. n., Longizonitis Pan and Bologna, 2018, Stenoria cf. grandiceps, and Ctenopus cf. persicus. Full article
(This article belongs to the Section Insect Systematics, Phylogeny and Evolution)
Show Figures

Figure 1

18 pages, 14379 KiB  
Article
Exploring Ecological Management Plans for Typical Systems in Arid Areas from the Perspective of Ecosystem Service Value Evolution
by Litang Yao, Xuebin Zhang, Jiale Yu, Yanni Liu, Hucheng Du and Xuehong Li
Systems 2024, 12(5), 166; https://doi.org/10.3390/systems12050166 (registering DOI) - 08 May 2024
Abstract
Ecological management zoning plays a significant role in optimizing resource utilization, improving ecosystem service function, and promoting coordinated regional development. Taking Hexi Corridor as a representative region of the Mountain–Oasis–Desert composite system in arid regions of Asia, this study analyzed the spatial and [...] Read more.
Ecological management zoning plays a significant role in optimizing resource utilization, improving ecosystem service function, and promoting coordinated regional development. Taking Hexi Corridor as a representative region of the Mountain–Oasis–Desert composite system in arid regions of Asia, this study analyzed the spatial and temporal evolution of ecosystem service values and explored the influencing mechanism based on the optimal parameters-based geographical detector model. We have comprehensively divided ecological management zones and proposed corresponding control strategies. The results show that (1) the Hexi Corridor is characterized by regional differentiation, which is composed of three systems: The southern mountain system, central oasis system, and northern desert system. The mountain system is mainly composed of forestland and grassland, the oasis system is mainly composed of cropland, and the desert system is mainly composed of unused land. The conversion of land use mainly involves the conversion of unused land to cropland and grassland, while grassland is mainly converted to cropland. (2) The ecosystem service value of the Hexi Corridor increased significantly and demonstrated agglomeration characteristics in space. The highest value areas are mainly distributed in the southern mountain, with higher value and medium areas mainly distributed in the central oasis, and the lowest value areas are mainly located in the northern desert. (3) Socio-economic factors greatly influence the spatial differentiation of ecosystem service values in the Hexi Corridor, with natural environmental factors having less impact. Additionally, the internal interaction of natural environmental factors is the most significant. (4) The Hexi Corridor is divided into three ecological management zones: Ecological function protection zone, ecological and agricultural coordinated development zone, and ecological and urbanization coordinated development zone. This research has important reference value for global ecological management in arid regions. Full article
Show Figures

Figure 1

10 pages, 755 KiB  
Article
The Value of Adding Exosome-Based Prostate Intelliscore to Multiparametric Magnetic Resonance Imaging in Prostate Biopsy: A Retrospective Analysis
by Noah King, Jacob Lang, Sree Jambunathan, Conner Lombardi, Barbara Saltzman, Nadiminty Nagalakshmi and Puneet Sindhwani
Uro 2024, 4(2), 50-59; https://doi.org/10.3390/uro4020005 (registering DOI) - 08 May 2024
Abstract
Introduction: Currently, there is limited evidence for the relationship of Exosome-based Prostate Intelliscore (EPI) and multiparametric magnetic resonance imaging (mpMRI) in stratifying risk for clinically significant prostate cancer. Using a retrospective cohort study design, we sought to characterize the relationship between these two [...] Read more.
Introduction: Currently, there is limited evidence for the relationship of Exosome-based Prostate Intelliscore (EPI) and multiparametric magnetic resonance imaging (mpMRI) in stratifying risk for clinically significant prostate cancer. Using a retrospective cohort study design, we sought to characterize the relationship between these two noninvasive metrics and prostate biopsy outcome. Methods: Data were collected via electronic medical record for all patients who underwent EPI testing from 1 January 2019 to 3 February 2022 and had available medical records at a single mid-western university medical center. Positive test result was defined as >15.6 for EPI, ≥3 PI-RADS score and ≥3 + 4 Gleason Score for biopsy findings. Utility of EPI, mpMRI and combined use was characterized through calculation of sensitivity, specificity, positive predictive value, negative predictive value, and ROC analysis. Results: A total of 226 patients were identified as receiving EPI testing for risk stratification of clinically significant prostate cancer. Sensitivity for EPI was 91%, mpMRI was 90%, and the highest was combined use at 96%. With ROC analysis, AUC for EPI alone was 0.57 (95% CI, 0.47–0.67) and 0.78 (95% CI, 0.70–0.87) for mpMRI alone. With prior positive EPI result, AUC for combined use with mpMRI was 0.80 (95% CI, 0.71–0.89). Further subgroup analysis resulted in increased AUC values of EPI 0.67 (95% CI, 0.48–0.87), mpMRI 0.90 (95% CI, 0.76–1.0), and combined 0.90 (95% CI, 0.75–1.0) in the African American population. Discussion: We observed that the combined use of EPI and mpMRI led to an avoided biopsy in 43% of patients. Using both parameters increased the overall sensitivity and diagnostic accuracy in detecting clinically significant prostate cancer. The best test performance was observed in the African American cohort. Identifying optimal noninvasive tools to assess risk for prostate cancer is crucial to providing accurate and cost-effective care. Future study should utilize a prospective study design to further support the combined use of these metrics. Full article
(This article belongs to the Special Issue Urologic Oncology: Analysis and Treatment)
Show Figures

Figure 1

16 pages, 5988 KiB  
Review
Economic and Social Impact of Huanglongbing on the Mexico Citrus Industry: A Review and Future Perspectives
by Hernán Villar-Luna, María Elena Santos-Cervantes, Edgar Antonio Rodríguez-Negrete, Jesús Méndez-Lozano and Norma Elena Leyva-López
Horticulturae 2024, 10(5), 481; https://doi.org/10.3390/horticulturae10050481 (registering DOI) - 08 May 2024
Abstract
The citrus industry is of great importance in Mexico, with an estimated production value of USD 2.4 billion and the potential to generate up to 28 million employees per year. Approximately 69,000 Mexican families depend on this sector. However, it is under serious [...] Read more.
The citrus industry is of great importance in Mexico, with an estimated production value of USD 2.4 billion and the potential to generate up to 28 million employees per year. Approximately 69,000 Mexican families depend on this sector. However, it is under serious threat from the disease known as Huanglongbing (HLB). The rapid spread of this disease has caused significant economic losses, impacted the livelihoods of citrus growers, and led to substantial job losses. Currently, HLB is not under control in Mexico, and as the management strategies applied have proven to be ineffective, yields have not been recovered to pre-HLB levels, and production costs have significantly increased. Therefore, it remains the most destructive citrus disease. This review paper describes the current situation of the disease, its economic and social impact, and the measures adopted for its management in Mexico. Future perspectives highlight advances in research based on new biotechnological tools as an eco-friendly management alternative. Practical suggestions to address HLB are also mentioned in our analysis. Full article
(This article belongs to the Special Issue The Diagnosis, Management, and Epidemiology of Plant Diseases)
Show Figures

Figure 1

11 pages, 1230 KiB  
Review
Targeted Therapy of Multiple Sclerosis: A Case for Antigen-Specific Tregs
by Yiya Zhong and Hans J. Stauss
Cells 2024, 13(10), 797; https://doi.org/10.3390/cells13100797 (registering DOI) - 08 May 2024
Abstract
Multiple sclerosis is an autoinflammatory condition that results in damage to myelinated neurons in affected patients. While disease-modifying treatments have been successful in slowing the progression of relapsing–remitting disease, most patients still progress to secondary progressive disease that is largely unresponsive to disease-modifying [...] Read more.
Multiple sclerosis is an autoinflammatory condition that results in damage to myelinated neurons in affected patients. While disease-modifying treatments have been successful in slowing the progression of relapsing–remitting disease, most patients still progress to secondary progressive disease that is largely unresponsive to disease-modifying treatments. Similarly, there is currently no effective treatment for patients with primary progressive MS. Innate and adaptive immune cells in the CNS play a critical role in initiating an autoimmune attack and in maintaining the chronic inflammation that drives disease progression. In this review, we will focus on recent insights into the role of T cells with regulatory function in suppressing the progression of MS, and, more importantly, in promoting the remyelination and repair of MS lesions in the CNS. We will discuss the exciting potential to genetically reprogram regulatory T cells to achieve immune suppression and enhance repair locally at sites of tissue damage, while retaining a fully competent immune system outside the CNS. In the future, reprogramed regulatory T cells with defined specificity and function may provide life medicines that can persist in patients and achieve lasting disease suppression after one cycle of treatment. Full article
(This article belongs to the Special Issue Advances in Cellular and Molecular Treatment of Autoimmune Diseases)
Show Figures

Figure 1

15 pages, 1694 KiB  
Article
Introducing a Novel Concept for an Integrated Demolition Waste Recycling Center and the Establishment of a Stakeholder Network: A Case Study from Germany
by Magdalena Zabek, Pauline Jegen and Lillith Kreiss
Sustainability 2024, 16(10), 3916; https://doi.org/10.3390/su16103916 (registering DOI) - 08 May 2024
Abstract
Using recycled aggregates has many positive environmental impacts because of the conservation of natural resources and minimization of waste. The use of recycled aggregates in downcycling processes is already common in Germany, whereas utilizing them to produce high-quality recycled concrete is rarely applied [...] Read more.
Using recycled aggregates has many positive environmental impacts because of the conservation of natural resources and minimization of waste. The use of recycled aggregates in downcycling processes is already common in Germany, whereas utilizing them to produce high-quality recycled concrete is rarely applied in practice. The reasons behind this lag have been investigated based on surveys and interviews with stakeholders. Miscommunication and missing information were identified in all stakeholder groups. Therefore, establishing a robust network and facilitating knowledge transfer by specifying the demand for recycled aggregates in the case study region have been considered as prerequisites. Therefore, the paper presents a novel concept of a stakeholder network for an integrated construction and demolition waste center. The conceptualization integrates the recycling companies and construction product manufacturers in one venue with research, service, and educational divisions. The design of the facilities is based on calculations regarding future construction activities and the demand for concrete production. The proposed concept aims to supply the region in the west of Germany with high-quality recycled products while also establishing a robust network that offers benefits in terms of logistical optimization and knowledge transfer. Full article
Show Figures

Figure 1

16 pages, 7426 KiB  
Article
Elasto-Plastic Analysis of Two-Way Reinforced Concrete Slabs Strengthened with Carbon Fiber Reinforced Polymer Laminates
by Zahraa Saleem Sharhan and Majid Movahedi Rad
Computation 2024, 12(5), 93; https://doi.org/10.3390/computation12050093 (registering DOI) - 08 May 2024
Abstract
This study explores a technique for enhancing the punching strength of reinforced concrete (RC) flat slabs, namely carbon fiber reinforced polymer (CFRP). Four large-scale RC flat slabs were fabricated, to assess the efficacy of this strengthening method. One slab served as a reference [...] Read more.
This study explores a technique for enhancing the punching strength of reinforced concrete (RC) flat slabs, namely carbon fiber reinforced polymer (CFRP). Four large-scale RC flat slabs were fabricated, to assess the efficacy of this strengthening method. One slab served as a reference and the three other specimens were strengthened with CFRP, as a method of external strengthening. These slabs, featuring identical overall dimensions and flexural steel reinforcement, underwent testing until failure, under the influence of concentrated patch loads. A concrete plastic damage constitutive model (CDP) was developed and employed to examine the strength of two-way RC slabs. Additionally, to enhance the strength of existing RC slabs, carbon fiber reinforced polymer (CFRP) strips are affixed to the tension surface of the sections. The research begins with the calibration of a numerical model, based on data from laboratory tests. The objective of this study is to constrain the plastic behavior of two-way RC slabs reinforced with CFRP, with a focus on establishing an optimal elasto-plastic analysis, aimed at controlling concrete damage plasticity using CFRP, and employing a plastic limit load multiplier. Subsequently, a series of numerical simulations, incorporating different variables, are conducted to investigate shear behavior. The numerical results indicate that an increase in the strengthening ratio has a significant impact on shear strength. Finite element simulations are carried out using Abaqus CAE®/2018. Full article
(This article belongs to the Section Computational Engineering)
Show Figures

Figure 1

7 pages, 1898 KiB  
Communication
Synthesis and Structure of a Coordination Polymer of Ni(II) with 2-(4-Bromophenoxy)acetohydrazide
by Оlena Martsynko, Mariia Nesterkina, Оlena Finik, Kyrylo Tsymbaliuk, Viktoriya Dyakonenko, Svitlana Shishkina and Iryna Kravchenko
Molbank 2024, 2024(2), M1819; https://doi.org/10.3390/M1819 (registering DOI) - 08 May 2024
Abstract
The coordination compound [NiCl2L(2-PrOH)]n (where L is 2-(4-bromophenoxy)acetohydrazide; 2-PrOH is isopropanol) was obtained for the first time. The complex was characterized by means of elemental analyses, molar conductance, thermogravimetric analysis, IR spectroscopy, and single crystal X-ray diffraction analysis. It was [...] Read more.
The coordination compound [NiCl2L(2-PrOH)]n (where L is 2-(4-bromophenoxy)acetohydrazide; 2-PrOH is isopropanol) was obtained for the first time. The complex was characterized by means of elemental analyses, molar conductance, thermogravimetric analysis, IR spectroscopy, and single crystal X-ray diffraction analysis. It was determined that the coordination compound exhibits a polymeric structure. The complexing agent is six-coordinated, and its distorted octahedron forms due to the bidentate coordination of 2-(4-bromophenoxy)acetohydrazide through the carbonyl oxygen atom and the amine nitrogen. The oxygen of the molecule of isopropanol, the chlorine atom, and two chlorine atoms serve as bridges between two metal atoms. Full article
Show Figures

Figure 1

14 pages, 5622 KiB  
Article
Identification of Damping of Spruce Wood (Picea abies) under Various Levels of Moisture Content Using Time-Scale Decomposition
by Miran Merhar
Polymers 2024, 16(10), 1313; https://doi.org/10.3390/polym16101313 (registering DOI) - 08 May 2024
Abstract
The damping of spruce wood is analysed at different moisture content levels for the first three vibration modes of tangentially and radially vibrating samples. Two methods were used to determine the damping. The first was the vibration envelope fitting as an improved version [...] Read more.
The damping of spruce wood is analysed at different moisture content levels for the first three vibration modes of tangentially and radially vibrating samples. Two methods were used to determine the damping. The first was the vibration envelope fitting as an improved version of the well-known logarithmic decrement, and the second was the newer and recently increasingly used wavelet transform. Both methods showed that the damping of spruce wood first decreases and then increases with moisture content, with the damping in the first vibration mode being about 9% higher in the radial direction than in the tangential direction. In the second and third vibration modes, the damping in the tangential direction was higher than in the radial direction by about 10% and 8.8%, respectively. The measured damping factors from the envelope fitting had, on average, 15.9% higher values than those from the wavelet transform. It can be concluded from the results that the wavelet transform is more accurate for determining the damping factor, as it enables the decoupling of multi-degree of freedom systems if mode coupling is present. Full article
(This article belongs to the Special Issue Advances in Wood-Based Materials and Wood Polymer Composites)
Show Figures

Figure 1

Open Access Journals

Browse by Indexing Browse by Subject Selected Journals
Back to TopTop