Intestinal homeostasis results from the proper interplay among epithelial cells, the enteric nervous system (ENS), interstitial cells of Cajal (ICCs), smooth muscle cells, the immune system, and the microbiota. The disruption of this balance underpins the onset of gastrointestinal-related diseases. The scarcity of models replicating the intricate interplay between the ENS and the intestinal epithelium highlights the imperative for developing novel methods. We have pioneered a sophisticated tridimensional in vitro technique, coculturing small intestinal organoids with myenteric and submucosal neurons. Notably, we have made significant advances in (1) refining the isolation technique for culturing the myenteric plexus, (2) enhancing the isolation of the submucosal plexus—both yielding mixed cultures of enteric neurons and glial cells from both plexuses, and (3) subsequently co-culturing myenteric and submucosal neurons with small intestinal organoids. This co-culture system establishes neural innervations with intestinal organoids, allowing for the investigation of regulatory interactions in the context of gastrointestinal diseases. Furthermore, we have developed a method for microinjecting the luminal space of small intestinal organoids with fluorescently labeled compounds. This technique possesses broad applicability such as the assessment of intestinal permeability, transcytosis, and immunocytochemical and immunofluorescence applications. This microinjection method could be extended to alternative experimental setups, incorporating bacterial species, or applying treatments to study ENS-small intestinal epithelium interactions. Therefore, this technique serves as a valuable tool for evaluating the intricate interplay between neuronal and intestinal epithelial cells (IECs) and shows great potential for drug screening, gene editing, the development of novel therapies, the modeling of infectious diseases, and significant advances in regenerative medicine. The co-culture establishment process spans twelve days, making it a powerful asset for comprehensive research in this critical field. Full article

Leaf chlorophyll content (LCC) is an important physiological index to evaluate the photosynthetic capacity and growth health of crops. In this investigation, the focus was placed on the chlorophyll content per unit of leaf area (LCC_A) and the chlorophyll content per unit of fresh weight (LCC_W) during the tuber formation phase of potatoes in Northern Shaanxi. Ground-based hyperspectral data were acquired for this purpose to formulate the vegetation index. The correlation coefficient method was used to obtain the “trilateral” parameters with the best correlation between potato LCC_A and LCC_W, empirical vegetation index, any two-band vegetation index constructed after 0–2 fractional differential transformation (step size 0.5), and the parameters with the highest correlation among the three spectral parameters, which were divided into four combinations as model inputs. The prediction models of potato LCC_A and LCC_W were constructed using the support vector machine (SVM), random forest (RF) and back propagation neural network (BPNN) algorithms. The results showed that, compared with the “trilateral” parameter and the empirical vegetation index, the spectral index constructed by the hyperspectral reflectance after differential transformation had a stronger correlation with potato LCC_A and LCC_W. Compared with no treatment, the correlation between spectral index and potato LCC and the prediction accuracy of the model showed a trend of decreasing after initial growth with the increase in differential order. The highest correlation index after 0–2 order differential treatment is DI, and the maximum correlation coefficients are 0.787, 0.798, 0.792, 0.788 and 0.756, respectively. The maximum value of the spectral index correlation coefficient after each order differential treatment corresponds to the red edge or near-infrared band. A comprehensive comparison shows that in the LCC_A and LCC_W estimation models, the RF model has the highest accuracy when combination 3 is used as the input variable. Therefore, it is more recommended to use the LCC_A to estimate the chlorophyll content of crop leaves in the agricultural practices of the potato industry. The results of this study can enhance the scientific understanding and accurate simulation of potato canopy spectral information, provide a theoretical basis for the remote sensing inversion of crop growth, and promote the development of modern precision agriculture. Full article

Exoskeletons are being explored for assisting motion therapy for neurological impairment-related rehabilitation. Soft robotic exoskeletons are gaining more attention for upper-extremity applications due to their simplistic actuation mechanisms and compliant nature. To regain fine motor hand functions, it is desired to have both hand and wrist motions in a coordinated fashion, as most daily living tasks require a combination of both hand and wrist joint motions. However, a soft robotic exoskeleton with hand and wrist motion together is an underdeveloped area. This paper presents a pneumatically actuated soft robotic exoskeleton designed to provide coordinated assistive motion to the hand and wrist joints using PD-based feedback control. The results showed the potential of the exoskeleton to provide flexion/extension rehabilitation exercises and task-oriented rehabilitation practices. Additionally, the results have confirmed that the implemented PD control ensures that the exoskeleton reaches the targeted angular trajectories and velocities. Two modes, full and partial assistance, were successfully tested to verify the ability of the exoskeleton to accommodate varying levels of impairment. Full article

The recognition of the Aerococcus urinae complex (AUC) as an emerging uropathogen has led to growing concerns due to a limited understanding of its disease spectrum and antibiotic resistance profiles. Here, we investigated the prevalence of macrolide resistance within urinary AUC isolates, shedding light on potential genetic mechanisms. Phenotypic testing revealed a high rate of macrolide resistance: 45%, among a total of 189 urinary AUC isolates. Genomic analysis identified integrative and conjugative elements (ICEs) as carriers of the macrolide resistance gene ermA, suggesting horizontal gene transfer as a mechanism of resistance. Furthermore, comparison with publicly available genomes of related pathogens revealed high ICE sequence homogeneity, highlighting the potential for cross-species dissemination of resistance determinants. Understanding mechanisms of resistance is crucial for developing effective surveillance strategies and improving antibiotic use. Furthermore, the findings underscore the importance of considering the broader ecological context of resistance dissemination, emphasizing the need for community-level surveillance to combat the spread of antibiotic resistance within the urinary microbiome. Full article

The main objective of this work is to present a comprehensive and integrated methodology to enhance the resilience of transportation critical infrastructure (TCI), focusing on the interplay between geotechnical assets and the transport network. Societies are greatly dependent on transport infrastructure systems, and as the mobility of passengers and the transport of freight is continuously growing, a disruption due to natural or man-made hazards creates significant impacts and dysfunctionalities on their operation and necessitates response measures to minimize vulnerability and ensure continuous functionality and robustness through resilience. Therefore, resilience quantification allows the design of ad hoc operation action plans before, during, and after a disruption, considering the dynamics of societal, ecological, and technological (SET) environments. The current work focuses on resilience quantification methodologies for TCIs and on the influence of single geotechnical asset (i.e., slope failure) resilience capacity on the overall system (i.e., national road network) resilience. Two case studies of unexpected transport network disruptions that took place in Greece are presented, and resilience metrics and performance indicators are applied to quantify the influence of the recovery stage. Full article

Monolayer (CrN, AlTiN) and bilayer (CrN/AlTiN) coatings are formed on the surface of conventional heat-treated and gas-nitrided X45CrMoV5-3-1 tool steel via Cathodic Arc Physical Vapor Deposition (CAPVD), and the adhesion characteristics and room- and high-temperature wear behavior of the coatings are compared with those of the un-nitrided ones. Scratch tests on the coatings show that the bilayer coating exhibits better adhesion behavior compared to monolayer ones, and the adhesion is further increased in all coatings due to the high load carrying capacity of the diffusion layer formed by the nitriding process. Dry friction tests performed at room temperature reveal that, among ceramic-based coatings, the coating system with a high adhesion has the lowest specific wear rate (0.06 × 10⁻⁶ mm³/N·m), and not only the surface hardness but also the nitriding process is important for reducing this rate. Studies on wear surfaces indicate that the bilayer coating structure has a tendency to remove the surface over a longer period of time. Hot wear tests performed at a temperature (450 °C) corresponding to aluminum extrusion conditions show that high friction coefficient values (>1) are reached due to aluminum transfer from the counterpart material to the surface and failure develops through droplet delamination. Adhesion and tribological tests indicate that the best performance among the systems studied belongs to the steel–CrN/AlTiN system and this performance can be further increased via the nitriding process. Full article

Many countries use low-cost sensors for high-resolution monitoring of particulate matter (PM_2.5 and PM₁₀) to manage public health. To enhance the accuracy of low-cost sensors, studies have been conducted to calibrate them considering environmental variables. Previous studies have considered various variables to calibrate seasonal variations in the PM concentration but have limitations in properly accounting for seasonal variability. This study considered the meridian altitude to account for seasonal variations in the PM concentration. In the PM₁₀ calibration, we considered the calibrated PM_2.5 as a subset of PM₁₀. To validate the proposed methodology, we used the feedforward neural network, support vector machine, generalized additive model, and stepwise linear regression algorithms to analyze the results for different combinations of input variables. The inclusion of the meridian altitude enhanced the accuracy and explanatory power of the calibration model. For PM_2.5, the combination of relative humidity, temperature, and meridian altitude yielded the best performance, with an average R² of 0.93 and root mean square error of 5.6 µg/m³. For PM₁₀, the average mean absolute percentage error decreased from 27.41% to 18.55% when considering the meridian altitude and further decreased to 15.35% when calibrated PM_2.5 was added. Full article

The accuracy of cosmic ray observations by the Large High Altitude Air Shower Observatory Wide Field-of-View Cherenkov/Fluorescence Telescope Array (LHAASO-WFCTA) is influenced by variations in aerosols in the atmosphere. The solar photometer (CE318-T) is extensively utilized within the Aerosol Robotic Network as a highly precise and reliable instrument for aerosol measurements. With this CE318-T 23, 254 sets of valid data samples over 394 days from October 2020 to October 2022 at the LHAASO site were obtained. Data analysis revealed that the baseline Aerosol Optical Depth (AOD) and Ångström Exponent (AE) at 440–870 nm (AE_440–870nm) of the aerosols were calculated to be 0.03 and 1.07, respectively, suggesting that the LHAASO site is among the most pristine regions on Earth. The seasonality of the mean AOD is in the order of spring > summer > autumn = winter. The monthly average maximum of AOD_440nm occurred in April (0.11 ± 0.05) and the minimum was in December (0.03 ± 0.01). The monthly average of AE_440–870nm exhibited slight variations. The seasonal characterization of aerosol types indicated that background aerosol predominated in autumn and winter, which is the optimal period for the absolute calibration of the WFCTA. Additionally, the diurnal daytime variations of AOD and AE across the four seasons are presented. Our analysis also indicates that the potential origins of aerosol over the LHAASO in four seasons were different and the atmospheric aerosols with higher AOD probably originate mainly from Northern Myanmar and Northeast India regions. These results are presented for the first time, providing a detailed analysis of aerosol seasonality and origins, which have not been thoroughly documented before in this region, also enriching the valuable materials on aerosol observation in the Hengduan Mountains and Tibetan Plateau. Full article

Rare earth elements comprise a group of 17 chemically similar elements, which increases the difficulty of separating them by traditional methods. For this reason, hydrometallurgy has been the most used method. However, it is important to evaluate the efficiency of the leaching processes used because, in addition to depending on the operating parameters of the leaching, they also depend on the mineralogical composition of the sample. In the present work, the extraction of Ce and La contained in the ferrocarbonatite mineral from the north of Mexico was studied. For the leaching tests, several leaching agents were used (HCl, H₂SO₄, HNO₃, and H₃PO₄ in different concentrations (0.5 [M], 1 [M], 1.5 [M]) and the temperature was modified to 20, 40, and 60 °C. A maximum recovery of 70% for Ce and La was obtained using HCl 1M in 4 h. The results of the kinetic study of the experiments showed that the best fitting model according to these kinetic models was the SCM controlled by a chemical reaction. Full article

Blockchain technology, initially developed as a decentralized and transparent mechanism for recording transactions, faces significant privacy challenges due to its inherent transparency, exposing sensitive transaction data to all network participants. This study proposes a blockchain privacy protection algorithm that employs a digital mutual trust mechanism integrated with advanced cryptographic techniques to enhance privacy and security in blockchain transactions. The contribution includes the development of a new dynamic Byzantine consensus algorithm within the Practical Byzantine Fault Tolerance framework, incorporating an authorization mechanism from the reputation model and a proof consensus algorithm for robust digital mutual trust. Additionally, the refinement of homomorphic cryptography using the approximate greatest common divisor technique optimizes the encryption process to support complex operations securely. The integration of a smart contract system facilitates automatic and private transaction execution across the blockchain network. Experimental evidence demonstrates the superior performance of the algorithm in handling privacy requests and transaction receipts with reduced delays and increased accuracy, marking a significant improvement over existing methods. Full article

For the first time, an attempt has been made to present, chronologically and in a systematized form, the development of ornithological studies at the National Museum of Natural History in Sofia (Bulgaria) from its foundation in the last decades of the 19th century to the present day. This 135-year period (1889–2024) includes the work of 12 curators of the ornithological collections. Their major contributions and most-significant ornithological publications are also presented. Full article

Existing direct and inverse kinematic models of planar parallel robots assume that the robot’s active joints are all at the bases. However, this approach becomes excessively complex when modeling a planar parallel robot in which the active joints are within one single kinematic chain. To address this problem, our article unveils an alternative for a 3RRR symmetric planar robot modeling technique for the derivation of the robot workspace and the analysis of its direct and inverse kinematics. The workspace was defined using a system of inequalities, and the direct and inverse kinematics models were generated using vectorial analysis and an optimized geometrical approach, respectively. The resulting models are systematically presented and validated. Two final model renditions are delivered supplying a thorough equation analysis and an applicability discussion based on the importance of the robot’s mobile platform orientation. The advantages of this model are discussed in comparison to the traditional modeling approach: whereas conventional techniques require the solution of complex eighth-degree polynomials for the analysis of the active joint configuration of these robots, these models provide an efficient back-of-the-envelope analysis approach that requires the solution of a simple second-degree polynomial. Full article

The possible relationship between Subjective Cognitive Decline (SCD) and dementia needs further investigation. In the present study, we explored the association between specific biomarkers of Alzheimer’s Disease (AD), amyloid-beta 42 (Aβ₄₂) and Tau with the odds of SCD using data from two ongoing studies. In total, 849 cognitively normal (CN) individuals were included in our analyses. Among the participants, 107 had available results regarding cerebrospinal fluid (CSF) Aβ₄₂ and Tau, while 742 had available genetic data to construct polygenic risk scores (PRSs) reflecting their genetic predisposition for CSF Aβ₄₂ and plasma total Tau levels. The associations between AD biomarkers and SCD were tested using logistic regression models adjusted for possible confounders such as age, sex, education, depression, and baseline cognitive test scores. Abnormal values of CSF Aβ₄₂ were related to 2.5-fold higher odds of SCD, while higher polygenic loading for Aβ₄₂ was associated with 1.6-fold higher odds of SCD. CSF Tau, as well as polygenic loading for total Tau, were not associated with SCD. Thus, only cerebral amyloidosis appears to be related to SCD status, either in the form of polygenic risk or actual CSF measurements. The temporal sequence of amyloidosis being followed by tauopathy may partially explain our findings. Full article

Background: The influence of medical and dental factors on jaw clicking within the South Australian population remains unexplored, and there is a lack of research on the impact of occlusal therapy on dynamic jaw movement parameters in this population. Purpose: This study investigated the potential significant associations between specific aspects of patient histories, occlusal therapy, and self-reported or observed jaw clicking in a population from the state of South Australia. Methods: Seventy individuals aged 18 to 65 participated in a cross-sectional study. Data collection included comprehensive medical, social, and dental histories, followed by evaluations of jaw function. Twenty-six out of the seventy individuals reported jaw clicking. Electrognathography assessed maximum mouth opening, surface electromyography evaluated masticatory muscle function, and joint vibration analysis measured individual joint vibration amplitudes and integrals. Logistic regression models analysed overall variable effects, while sub-models focused on predictors related to occlusal therapy, specifically orthodontic intervention. Independent t-tests and Mann–Whitney U tests compared jaw functions between participants who received occlusal therapy and those who did not. Results: The number of third molars extracted, vitamin D deficiency, and self-reported mental health disorders (R² = 0.414, p = 0.048) emerged as significant predictors for jaw clicking. Factors associated with occlusal therapy showed no significant association with jaw clicking (R² = 0.59, p = 0.027). Furthermore, there were no significant differences observed in mouth opening (t-stat = −0.439, p = 0.662), muscle activity, and joint vibration analysis between participants who underwent occlusal therapy and those who did not. Conclusions: Within the selected study population and limited sample size, the number of third molars extracted, vitamin D deficiency, and self-reported mental health disorders were associated with jaw clicking. Full article

E-commerce platforms incorporate reputation systems that allow buyers to rate sellers after transactions. However, existing reputation systems face challenges such as privacy leakage, linkability, and multiple rating attacks. The feedback data can inadvertently expose user information privacy because they reveal the buyers’ identities and preferences, which deters a significant number of users from providing their ratings. Moreover, malicious actors can exploit data analysis and machine learning techniques to mine user privacy from the rating data, posing serious threats to user security and trust. This study introduces ARS-Chain, a pioneering and secure blockchain-driven anonymous reputation-sharing framework tailored for e-commerce platforms. The core of ARS-Chain is a dynamic ring addition mechanism with linkable ring signatures (LRS), where the number of LRS rings is dynamically added in alignment with the evolving purchase list, and LRS link tags are constructed with the LRS rings and item identifiers. Further, a consortium blockchain is introduced to store these anonymous ratings on e-commerce platforms. As a result, ARS-Chain ensures full anonymity while achieving cross-platform reputation sharing, making rating records unlinkable, and effectively countering multiple rating attacks. The experimental results confirm that ARS-Chain significantly enhances user information privacy protection while maintaining system performance, having an important impact on the construction of trust mechanisms for e-commerce platforms. Full article

The olive oil industry recently introduced a novel multi-phase decanter with the “Leopard DMF” series, which gives a by-product called pâté, made up of pulp and olive wastewater with a high content of phenolic substances and without pits. This study aims to create a new culture medium, the Olive Juice Broth (OJB), from DMF pâté, and apply it to select bacteria strains able to survive and degrade the bitter substances normally present in the olive fruit. Thirty-five different bacterial strains of Lactiplantibacillus plantarum from the CREA-IT.PE Collection of Microorganisms were tested. Seven strains characterized by ≥50% growth in OJB (B31, B137, B28, B39, B124, B130, and B51) showed a degradation of the total phenolic content of OJB ≥ 30%. From this set, L. plantarum B51 strain was selected as a starter for table olive production vs. spontaneous fermentation. The selected inoculant effectively reduced the debittering time compared to spontaneous fermentation. Hydroxytyrosol, derived from oleuropein and verbascoside degradation, and tyrosol, derived from ligstroside degradation, were produced faster than during spontaneous fermentation. The OJB medium is confirmed to be useful in selecting bacterial strains resistant to the complex phenolic environment of the olive fruit. Full article

Millions of years of isolation have given Madagascar a unique flora that still reflects some of its relationship with the continents of Africa and India. Here, the complete chloroplast sequence of Beilschmiedia moratii, a tropical tree in Madagascar, was determined. The plastome, with a length of 158,410 bp, was 143 bp and 187 bp smaller than those of two closely related species, B. pierreana and Potameia microphylla, in sub-Saharan Africa and Madagascar with published sequences, respectively. A total of 124 repeats and 114 simple sequence repeats (SSRs) were detected in the plastome of B. moratii. Six highly variable regions, including ndhF, ndhF-rpl32, trnC-petN, pebE-petL, rpl32-trnL, and ycf1, among the three African species were identified and 1151 mutation events, including 14 SVs, 351 indels, and 786 substitutions, were accurately located. There were 634 mutation events between B. moratii and P. microphylla with a mean nucleotide variability (π) value of 0.00279, while there were 827 mutation events between B. moratii and B. pierreana with a mean π value of 0.00385. The Ka/Ks ratios of 86 protein-coding genes in the three African species were less than 1, and the mean value between B. moratii and P. microphylla was 0.184, while the mean value between B. moratii and B. pierreana was 0.286. In this study, the plastid genomes of the three African Beilschmiediineae species were compared for the first time and revealed that B. moratii and P. microphylla from Madagascar were relatively conserved, with low mutation rates and slower evolutionary rates. Full article

Human health is dependent on food safety and, therefore, on the health of farm animals. One of the most significant threats in regard to swine diseases is African swine fever (ASF). Infections caused by porcine circoviruses (PCVs) represent another important swine disease. Due to the ubiquitous nature of PCV2, it is not surprising that this virus has been detected in ASFV-affected pigs. However, recent data indicate that coinfection of PCV3 and ASFV also occurs. It is still unclear whether PCV infection plays a role in ASFV infection, and that subject requires further analysis. The aim of this study was to assess whether PCV3 and PCV4 are present in the wild boar population in Poland (real-time PCR). The analysis was performed on wild boar samples collected for routine ASF surveillance in Poland, between 2018 and 2021. By extension, the obtained data were compared in regard to ASFV presence in these samples, thus investigating the odds of ASFV infection on the grounds of the PCV carrier state in free-ranging Suidae in Poland. In addition, sequencing of PCV3 and phylogenetic analysis were performed, based on a full genome and a capsid gene. In the current study, we demonstrated the high prevalence of PCV3 in the wild boar population in Poland; meanwhile, PCV4 was not detected. The odds of ASFV infection on the grounds of the PCV3 carrier state in free-ranging Suidae in Poland was more than twice as high. Ten full genome sequences of PCV3 were obtained, all of them belonging to clade 3a. The similarity between them was in the range of 98.78–99.80%. Full article

Photosynthetic microbial fuel cells (pMFCs) are hybrid systems that enable simultaneous wastewater treatment under anaerobic conditions and the generation of electricity by utilizing the potential difference in the anaerobic anode chamber and the oxygenated cathode chamber. Dairy wastewater with a concentration of 2000 mg COD/L was treated in the anode of a batch pMFC. In the cathode chamber, Chlorella vulgaris or Arthrospira platensis was cultivated in synthetic medium, and next in diluted effluent from the anode chamber. The highest power density of 91 mW/m² was generated by the pMFC with the cultivation of Arthrospira platensis. Higher values of dissolved oxygen remained during the dark phase in the cathodic medium with Arthrospira platensis cultivation than with Chlorella vulgaris. This depletion of oxygen significantly decreased voltage generation, which during the light phase increased again to the maximum values. The COD removal achieved in the anodic chamber was 87%. The efficiency of nitrogen removal in the cathode chamber during the cultivation of Arthrospira platensis and Chlorella vulgaris was about 78% and 69%, respectively. The efficiency of phosphorus removal in the cathode chamber with the cultivation of Arthrospira plantensis and Chlorella vulgaris was 58% and 43%, respectively. This study has shown that the introduction of Arthrospira platensis into the cathode chamber is more effective than that of Chlorella vulgaris. Full article

We studied the morphostructural features and chemical composition of micron and submicron particles of native gold from brown coals and overcoal sediments of the Yerkovetsky deposit (Zeya-Bureya sedimentary basin, Far East Russia). The samples of coal and host rocks in the form of thin sections, as well as coal particles and grains of native gold obtained during the process of dispersion and the fractionation of loose and crushed samples divided according to size and density, were analyzed using scanning electron microscopy in combination with X-ray microanalysis, involving various visualization modes. It was revealed that native gold is syngenetic with the mineralization of brown coals, and microphases dispersed in the minerals of overcoal loose and sandy-clay sediments were the source of native gold. In coal, gold is accumulated at the stages of formation (alluvial and eolian, including terrigenous and ionogenic subtypes) and the diagenesis of coal deposits (ground-infiltration subtype). A significant part of the mineralization process of coals and the formation of microparticles of native gold was contributed to by the descending water infiltration of polycomponent colloid solutions. During the dehydration of hydroxysiliconized iron-based hydrogels, mineral phases have an unstable composition and floccular structure and contain submicron gold particles. The coatings of all gold microparticles have identical origin and composition. Coal beds that border host rocks are an open system with a constant inflow of the substance, which leads to the gradual formation of polycomponent aggregated particles in micro cavities. Part of the gold in coals occurs as sulfur-bearing complexes dissolved in pore water. The key factor in the migration and deposition of gold in coals is the inorganic substances involved in the processes of coal mineralization. Organic substances play a more passive role and have medium-forming, fractionating (colloid, molecular, and ionic sieves), and accumulation functions. Full article

As a physical mutagen, carbon ion beam (CIB) irradiation can induce high-frequency mutation, which is user-friendly and environment-friendly in plant breeding. In this study, we resequenced eight mutant lines which were screened out from the progeny of the CIB-irradiated dehulled rice seeds. Among these mutants, CIB induced 135,535 variations, which include single base substitutions (SBSs), and small insertion and deletion (InDels). SBSs are the most abundant mutation, and account for 88% of all variations. Single base conversion is the main type of SBS, and the average ratio of transition and transversion is 1.29, and more than half of the InDels are short-segmented mutation (1–2 bp). A total of 69.2% of the SBSs and InDels induced by CIBs occurred in intergenic regions on the genome. Surprisingly, the average mutation frequency in our study is 9.8 × 10⁻⁵/bp and much higher than that of the previous studies, which may result from the relatively high irradiation dosage and the dehulling of seeds for irradiation. By analyzing the mutation of every 1 Mb in the genome of each mutant strain, we found some unusual high-frequency (HF) mutation regions, where SBSs and InDels colocalized. This study revealed the mutation mechanism of dehulled rice seeds by CIB irradiation on the genome level, which will enrich our understanding of the mutation mechanism of CIB radiation and improve mutagenesis efficiency. Full article

Lions (Panthera leo) play a crucial ecological role in shaping and maintaining fragile ecosystems within Africa. Conservation efforts should focus on genetic variability within wild populations when considering reintroduction attempts. We studied two groups of lions from two conservation sites located in Zambia and Zimbabwe to determine their genetic make-up, information that is usually unknown to the sites. In this study, we analysed 17 specimens for cytb and seven microsatellite markers to ascertain family relationships and genetic diversity previously obtained by observational studies. We then produced a standardised haplogroup phylogeny using all available entire mitogenomes, as well as calculating a revised molecular clock. The modern lion lineage diverged ~151 kya and was divided into two subspecies, both containing three distinct haplogroups. We confirm that Panthera leo persica is not a subspecies, but rather a haplogroup of the northern P.l. leo that exited Africa at least ~31 kya. The progenitor to all lions existed ~1.2 Mya, possibly in SE Africa, and later exited Africa and split into the two cave lion lineages ~175 kya. Species demography is correlated to major climactic events. We now have a detailed phylogeny of lion evolution and an idea of their conservation status given the threat of climate change. Full article