The 2023 MDPI Annual Report has
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8 pages, 2678 KiB  
Communication
Mindfulness in Facilitating Pelvic Floor Botulinum Toxin Injection in Women with Chronic Pelvic Pain
by Jacqueline V. Aredo, Hannah K. Tandon, Samin Panahi, Vy T. Phan, Rezvan Ameli, Barbara I. Karp and Pamela Stratton
Toxins 2024, 16(5), 216; https://doi.org/10.3390/toxins16050216 (registering DOI) - 9 May 2024
Abstract
Botulinum toxin (BoNT) injection can safely be done as an office-based procedure, but can be painful itself, especially when injecting pelvic floor muscles to treat chronic pelvic pain (CPP). Mindfulness interventions may reduce procedure-associated acute anxiety and pain. We applied mindfulness techniques to [...] Read more.
Botulinum toxin (BoNT) injection can safely be done as an office-based procedure, but can be painful itself, especially when injecting pelvic floor muscles to treat chronic pelvic pain (CPP). Mindfulness interventions may reduce procedure-associated acute anxiety and pain. We applied mindfulness techniques to increase the tolerability of office-based pelvic floor BoNT injections in women with CPP. Women enrolled in a clinical trial of BoNT for endometriosis-associated CPP were offered a brief, guided mindfulness session before and/or after transvaginal injection. Anxiety, pain, and dysphoria were rated on a 0–10 numerical rating scale (NRS) before and after each mindfulness session. Eight women underwent mindfulness sessions. Five participants had a session before and two after the transvaginal injection. One participant had two sessions: one before and one after separate injections. All six women completing a session prior to injection had at least moderate anxiety, which lessened after the mindfulness session (median NRS change: −3.3/10). All three women reporting injection-associated pain experienced less intense pain following the post-injection session (median NRS change: −3/10). Three women experiencing dysphoria improved after the session (median NRS change: −3/10). A brief, guided mindfulness session may lessen acute pain, anxiety, and dysphoria associated with office-based transvaginal BoNT injection. Full article
(This article belongs to the Special Issue Uses of Botulinum Toxin Injection in Medicine)
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18 pages, 5322 KiB  
Article
Improved Real-Time Models for Object Detection and Instance Segmentation for Agaricus bisporus Segmentation and Localization System Using RGB-D Panoramic Stitching Images
by Chenbo Shi, Yuanzheng Mo, Xiangqun Ren, Jiahao Nie, Chun Zhang, Jin Yuan and Changsheng Zhu
Agriculture 2024, 14(5), 735; https://doi.org/10.3390/agriculture14050735 (registering DOI) - 9 May 2024
Abstract
The segmentation and localization of Agaricus bisporus is a precondition for its automatic harvesting. A. bisporus growth clusters can present challenges for precise localization and segmentation because of adhesion and overlapping. A low-cost image stitching system is presented in this research, utilizing a [...] Read more.
The segmentation and localization of Agaricus bisporus is a precondition for its automatic harvesting. A. bisporus growth clusters can present challenges for precise localization and segmentation because of adhesion and overlapping. A low-cost image stitching system is presented in this research, utilizing a quick stitching method with disparity correction to produce high-precision panoramic dual-modal fusion images. An enhanced technique called Real-Time Models for Object Detection and Instance Segmentation (RTMDet-Ins) is suggested. This approach utilizes SimAM Attention Module’s (SimAM) global attention mechanism and the lightweight feature fusion module Space-to-depth Progressive Asymmetric Feature Pyramid Network (SPD-PAFPN) to improve the detection capabilities for hidden A. bisporus. It efficiently deals with challenges related to intricate segmentation and inaccurate localization in complex obstacles and adhesion scenarios. The technology has been verified by 96 data sets collected on a self-designed fully automatic harvesting robot platform. Statistical analysis shows that the worldwide stitching error is below 2 mm in the area of 1200 mm × 400 mm. The segmentation method demonstrates an overall precision of 98.64%. The planar mean positioning error is merely 0.31%. The method promoted in this research demonstrates improved segmentation and localization accuracy in a challenging harvesting setting, enabling efficient autonomous harvesting of A. bisporus. Full article
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22 pages, 1696 KiB  
Review
A Review of Artificial Intelligence in Breast Imaging
by Dhurgham Al-Karawi, Shakir Al-Zaidi, Khaled Ahmad Helael, Naser Obeidat, Abdulmajeed Mounzer Mouhsen, Tarek Ajam, Bashar A. Alshalabi, Mohamed Salman and Mohammed H. Ahmed
Tomography 2024, 10(5), 705-726; https://doi.org/10.3390/tomography10050055 (registering DOI) - 9 May 2024
Abstract
With the increasing dominance of artificial intelligence (AI) techniques, the important prospects for their application have extended to various medical fields, including domains such as in vitro diagnosis, intelligent rehabilitation, medical imaging, and prognosis. Breast cancer is a common malignancy that critically affects [...] Read more.
With the increasing dominance of artificial intelligence (AI) techniques, the important prospects for their application have extended to various medical fields, including domains such as in vitro diagnosis, intelligent rehabilitation, medical imaging, and prognosis. Breast cancer is a common malignancy that critically affects women’s physical and mental health. Early breast cancer screening—through mammography, ultrasound, or magnetic resonance imaging (MRI)—can substantially improve the prognosis for breast cancer patients. AI applications have shown excellent performance in various image recognition tasks, and their use in breast cancer screening has been explored in numerous studies. This paper introduces relevant AI techniques and their applications in the field of medical imaging of the breast (mammography and ultrasound), specifically in terms of identifying, segmenting, and classifying lesions; assessing breast cancer risk; and improving image quality. Focusing on medical imaging for breast cancer, this paper also reviews related challenges and prospects for AI. Full article
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19 pages, 5028 KiB  
Article
Accuracy of Simscape Solar Cell Block for Modeling a Partially Shaded Photovoltaic Module
by Tihomir Betti, Ante Kristić, Ivan Marasović and Vesna Pekić
Energies 2024, 17(10), 2276; https://doi.org/10.3390/en17102276 (registering DOI) - 9 May 2024
Abstract
With half-cut photovoltaic (PV) modules being the dominant technology on the market, there is an increasing necessity for accurate modeling of this module type. Circuit simulators such as Simulink are widely used to study different topics regarding photovoltaics, often employing a solar cell [...] Read more.
With half-cut photovoltaic (PV) modules being the dominant technology on the market, there is an increasing necessity for accurate modeling of this module type. Circuit simulators such as Simulink are widely used to study different topics regarding photovoltaics, often employing a solar cell block available from the Simcape library. The purpose of this work is to validate this model against measurements for a partially shaded half-cut PV module. Diverse shading scenarios are created by varying the number of shaded substrings, the number of shaded solar cells in the substring, and the shading level. For every shading scenario, the PV module’s I-V curve is measured, along with in-plane irradiance, air temperature, and module temperature. A comprehensive evaluation of simulation accuracy is presented. The results confirm a high accuracy of the model with mean nRMSE values of 2.2% for I-V curves and 2.8% when P-V curves are considered. It is found that the simulation errors tend to increase when increasing the number of shaded substrings. At the same time, no obvious dependency of simulation accuracy on the shading level or the number of shaded solar cells in the substring is found. Full article
(This article belongs to the Special Issue Advances in Solar Systems and Energy Efficiency)
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15 pages, 2189 KiB  
Article
Bulk Free Radical Terpolymerization of Butyl Acrylate, 2-Methylene-1,3-Dioxepane and Vinyl Acetate: Terpolymer Reactivity Ratio Estimation
by Maryam Movafagh, Kelly M. Meek, Alison J. Scott, Alexander Penlidis and Marc A. Dubé
Polymers 2024, 16(10), 1330; https://doi.org/10.3390/polym16101330 (registering DOI) - 9 May 2024
Abstract
This investigation introduces the first estimation of ternary reactivity ratios for a butyl acrylate (BA), 2-methylene-1,3-dioxepane (MDO), and vinyl acetate (VAc) system at 50 °C, with an aim to develop biodegradable pressure-sensitive adhesives (PSAs). In this study, we applied the error-in-variables model (EVM) [...] Read more.
This investigation introduces the first estimation of ternary reactivity ratios for a butyl acrylate (BA), 2-methylene-1,3-dioxepane (MDO), and vinyl acetate (VAc) system at 50 °C, with an aim to develop biodegradable pressure-sensitive adhesives (PSAs). In this study, we applied the error-in-variables model (EVM) to estimate reactivity ratios. The ternary reactivity ratios were found to be r12 = 0.417, r21 = 0.071, r13 = 4.459, r31 = 0.198, r23 = 0.260, and r32 = 55.339 (BA/MDO/VAc 1/2/3), contrasting with their binary counterparts, which are significantly different, indicating the critical need for ternary system analysis to accurately model multicomponent polymerization systems. Through the application of a recast Alfrey–Goldfinger model, this investigation predicts the terpolymer’s instantaneous and cumulative compositions at various conversion levels, based on the ternary reactivity ratios. These predictions not only provide crucial insights into the incorporation of MDO across different initial feed compositions but also offer estimates of the final terpolymer compositions and distributions, underscoring their potential in designing compostable or degradable polymers. Full article
(This article belongs to the Special Issue State-of-the-Art Polymer Science and Technology in Canada)
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22 pages, 15315 KiB  
Article
Study on Wind-Induced Dynamic Response and Statistical Parameters of Skeleton Supported Saddle Membrane Structure in Arching and Vertical Direction
by Ziye Chen, Changjiang Liu, Dong Li, Jian Liu, Xiaowei Deng, Chiyu Luo and Guangen Zhou
Buildings 2024, 14(5), 1339; https://doi.org/10.3390/buildings14051339 (registering DOI) - 9 May 2024
Abstract
Wind tunnel tests and numerical simulations are the mainstream methods to study the wind-induced vibration of structures. However, few articles use statistical parameters to point out the differences and errors of these two research methods in exploring the wind-induced response of membrane structures. [...] Read more.
Wind tunnel tests and numerical simulations are the mainstream methods to study the wind-induced vibration of structures. However, few articles use statistical parameters to point out the differences and errors of these two research methods in exploring the wind-induced response of membrane structures. The displacement vibration of a saddle membrane structure under the action of wind load is studied by wind tunnel tests and numerical simulation, and statistical parameters (mean, range, skewness, and kurtosis) are introduced to analyze and compare the displacement data. The most unfavorable wind direction angle is 0° (arching direction). The error between experiment and simulation is less than 10%. The probability density curve has a good coincidence degree. Both the test and simulation show a certain skewed distribution, indicating that the wind-induced vibration of the membrane does not obey the Gaussian distribution. The displacement response obtained by the test has good stability, while the simulated displacement response has strong discreteness. The difference between the two research methods is quantitatively given by introducing statistical parameters, which is helpful to improve the shortcomings of wind tunnel tests and numerical simulations. Full article
(This article belongs to the Special Issue Wind Load Effects on High-Rise and Long-Span Structures)
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27 pages, 40205 KiB  
Article
Deep Learning-Enhanced Sampling-Based Path Planning for LTL Mission Specifications
by Changmin Baek and Kyunghoon Cho
Sensors 2024, 24(10), 2998; https://doi.org/10.3390/s24102998 (registering DOI) - 9 May 2024
Abstract
The presented paper introduces a novel path planning algorithm designed for generating low-cost trajectories that fulfill mission requirements expressed in Linear Temporal Logic (LTL). The proposed algorithm is particularly effective in environments where cost functions encompass the entire configuration space. A core contribution [...] Read more.
The presented paper introduces a novel path planning algorithm designed for generating low-cost trajectories that fulfill mission requirements expressed in Linear Temporal Logic (LTL). The proposed algorithm is particularly effective in environments where cost functions encompass the entire configuration space. A core contribution of this paper is the presentation of a refined approach to sampling-based path planning algorithms that aligns with the specified mission objectives. This enhancement is achieved through a multi-layered framework approach, enabling a simplified discrete abstraction without relying on mesh decomposition. This abstraction is especially beneficial in complex or high-dimensional environments where mesh decomposition is challenging. The discrete abstraction effectively guides the sampling process, influencing the selection of vertices for extension and target points for steering in each iteration. To further improve efficiency, the algorithm incorporates a deep learning-based extension, utilizing training data to accurately model the optimal trajectory distribution between two points. The effectiveness of the proposed method is demonstrated through simulated tests, which highlight its ability to identify low-cost trajectories that meet specific mission criteria. Comparative analyses also confirm the superiority of the proposed method compared to existing methods. Full article
(This article belongs to the Topic Advances in Mobile Robotics Navigation, 2nd Volume)
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15 pages, 4414 KiB  
Article
Ti3AlC2 MAX Phase Modified Screen-Printed Electrode for the Fabrication of Hydrazine Sensor
by Khursheed Ahmad, Waseem Raza and Rais Ahmad Khan
Micromachines 2024, 15(5), 633; https://doi.org/10.3390/mi15050633 (registering DOI) - 9 May 2024
Abstract
Hydrazine is considered a powerful reducing agent and catalyst, showing diverse applications in agricultural industries, toxic degradation research, and wastewater management. Additionally, hydrazine can trigger some specific reactions when combined with suitable oxidants. Due to its highly polar nature, hydrazine can easily dissolve [...] Read more.
Hydrazine is considered a powerful reducing agent and catalyst, showing diverse applications in agricultural industries, toxic degradation research, and wastewater management. Additionally, hydrazine can trigger some specific reactions when combined with suitable oxidants. Due to its highly polar nature, hydrazine can easily dissolve in alcohol, water, and various other polar solvents. Therefore, it can be extensively utilized in different areas of application and industries such as rocketry and various chemical applications. Despite its beneficial properties, hydrazine is unstable, posing significant risk due to its highly toxic nature. It is extremely hazardous to both human health and the environment. It can cause various illnesses and symptoms such as dizziness, temporary blindness, damage to the central nervous system, and even death when inhaled in sufficient quantities. Therefore, it is highly important to monitor the level of hydrazine to prevent its toxic and hazardous effects on human beings and the environment. In the present study, we discuss the simple fabrication of a disposable cost-effective and eco-friendly hydrazine sensor. We used a screen-printed carbon electrode, i.e., SPCE, as a base for the construction of a hydrazine sensor. The Ti3AlC2 MAX has been used as a suitable and efficient electrode material for the fabrication of disposable hydrazine sensors. We modified the active surface of the SPCE using a drop-casting approach. The resulting Ti3AlC2 MAX modified SPCE (Ti3AlC2@SPCE) has been utilized as an efficient and low-cost hydrazine sensor. Cyclic voltammetry, i.e., CV, and linear sweep voltammetry, viz., LSV, was employed as a sensing technique in this study. The optimization of pH and electrode material loading was conducted. The Ti3AlC2@SPCE exhibited excellent sensing performance toward hydrazine oxidation. A reasonable detection limit (0.01 µM) was achieved for hydrazine sensing. The fabricated sensor also demonstrated a reasonable linear range of 1–50 µM. This work provides the design and fabrication of simple disposable Ti3AlC2@SPCE as a suitable electrode for the determination of hydrazine using LSV technology. Full article
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29 pages, 1895 KiB  
Article
An Efficient Attribute-Based Participant Selecting Scheme with Blockchain for Federated Learning in Smart Cities
by Xiaojun Yin, Haochen Qiu, Xijun Wu and Xinming Zhang
Computers 2024, 13(5), 118; https://doi.org/10.3390/computers13050118 (registering DOI) - 9 May 2024
Abstract
In smart cities, large amounts of multi-source data are generated all the time. A model established via machine learning can mine information from these data and enable many valuable applications. With concerns about data privacy, it is becoming increasingly difficult for the publishers [...] Read more.
In smart cities, large amounts of multi-source data are generated all the time. A model established via machine learning can mine information from these data and enable many valuable applications. With concerns about data privacy, it is becoming increasingly difficult for the publishers of these applications to obtain users’ data, which hinders the previous paradigm of centralized training through collecting data on a large scale. Federated learning is expected to prevent the leakage of private data by allowing users to train models locally. The existing works generally ignore architectures designed in real scenarios. Thus, there still exist some challenges that have not yet been explored in federated learning applied in smart cities, such as avoiding sharing models with improper parties under privacy requirements and designing satisfactory incentive mechanisms. Therefore, we propose an efficient attribute-based participant selecting scheme to ensure that only someone who meets the requirements of the task publisher can participate in training under the premise of high privacy requirements, so as to improve the efficiency and avoid attacks. We further extend our scheme to encourage clients to take part in federated learning and provide an audit mechanism using a consortium blockchain. Finally, we present an in-depth discussion of the proposed scheme by comparing it to different methods. The results show that our scheme can improve the efficiency of federated learning by enabling reliable participant selection and promote the extensive use of federated learning in smart cities. Full article
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13 pages, 5867 KiB  
Article
Determining Riverine Surface Roughness at Fluvial Mesohabitat Level and Its Influence on UAV-Based Thermal Imaging Accuracy
by Johannes Kuhn, Joachim Pander, Luis Habersetzer, Roser Casas-Mulet and Juergen Geist
Remote Sens. 2024, 16(10), 1674; https://doi.org/10.3390/rs16101674 (registering DOI) - 9 May 2024
Abstract
Water surface roughness (SR) is a highly relevant parameter governing data reliability in remote sensing applications, yet lacking appropriate methodology in riverine habitats. In order to assess thermal accuracy linked to SR of thermal imaging derived from an unmanned aerial vehicle (UAV), we [...] Read more.
Water surface roughness (SR) is a highly relevant parameter governing data reliability in remote sensing applications, yet lacking appropriate methodology in riverine habitats. In order to assess thermal accuracy linked to SR of thermal imaging derived from an unmanned aerial vehicle (UAV), we developed the SR Measurement Device (SRMD). The SRMD uses the concept of in situ quantification of wave frequency and wave amplitude. Data of nine installed SRMDs in four different fluvial mesohabitat classes presented a range of 0 to 47 waves per 30 s and an amplitude range of 0 to 6 cm. Even subtle differences between mesohabitat classes run, riffle, and no-/low-flow still and pool areas could be detected with the SRMD. However, SR revealed no significant influence on the accuracy of thermal infrared (TIR) imagery data in our study case. Overall, the presented device expands existing methods of riverine habitat assessments and has the potential to produce highly relevant data of SR for various ecological and technical applications, ranging from remote sensing of surface water and habitat quality characterizations to bank stability and erosion risk assessments. Full article
(This article belongs to the Special Issue Remote Sensing and GIS in Freshwater Environments)
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17 pages, 3863 KiB  
Review
Sixth-Generation (6G) Networks for Improved Machine-to-Machine (M2M) Communication in Industry 4.0
by Izabela Rojek, Piotr Kotlarz, Janusz Dorożyński and Dariusz Mikołajewski
Electronics 2024, 13(10), 1832; https://doi.org/10.3390/electronics13101832 (registering DOI) - 9 May 2024
Abstract
The sixth generation of mobile networks (6G) has the potential to revolutionize the way we communicate, interact, and use information for machine-to-machine (M2M) communication in Industry 4.0 and Industry 5.0, while also improving coverage in places that were previously considered difficult to access [...] Read more.
The sixth generation of mobile networks (6G) has the potential to revolutionize the way we communicate, interact, and use information for machine-to-machine (M2M) communication in Industry 4.0 and Industry 5.0, while also improving coverage in places that were previously considered difficult to access and/or digitally excluded, and supporting more devices and users. The 6G network will have an impact through a combination of many technologies: the Internet of Things (IoT), artificial intelligence/machine learning, virtual and augmented reality, cloud computing, and cyber security. New solutions and architectures and concepts for their use need to be developed to take full advantage of this. This article provides an overview of the challenges in this area and the proposed solutions, taking into account the disruptive technologies that are yet to be developed. Full article
(This article belongs to the Special Issue Smart Communication and Networking in the 6G Era)
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14 pages, 2159 KiB  
Article
Economic Aspects of Zinc Oxide Fertilization in Yam (Dioscorea alata L.) in a Semi-Arid Region of Brazil
by Antônio Lourenço Bezerra, João Everthon da Silva Ribeiro, Ester dos Santos Coêlho, Elania Freire da Silva, Pablo Henrique de Almeida Oliveira, Gisele Lopes dos Santos, Antonio Gideilson Correia da Silva, José Travassos dos Santos Júnior, Ivanice da Silva Santos, Felipe Alves Reis, Lindomar Maria da Silveira, Aurélio Paes Barros Júnior and Adriano do Nascimento Simões
Horticulturae 2024, 10(5), 489; https://doi.org/10.3390/horticulturae10050489 (registering DOI) - 9 May 2024
Abstract
The management and improvement of yam productivity are associated with a good supply of essential nutrients for the growth and development of the crop that has economic viability. This research aimed to evaluate the economic feasibility of foliar fertilization with Zintrac® in [...] Read more.
The management and improvement of yam productivity are associated with a good supply of essential nutrients for the growth and development of the crop that has economic viability. This research aimed to evaluate the economic feasibility of foliar fertilization with Zintrac® in two yam agricultural seasons (2022/2023 and 2023/2024). Therefore, two experiments were conducted at the Rafael Fernandes Experimental Farm, Mossoró, RN, Brazil. The experimental design was in a Latin square design with five treatments of doses of Zintrac® (0, 1, 2, 3, and 4 L ha−1) and five replications. Among the production costs of yams, labor and seed acquisition were the most significant. The highest profitability index was achieved with the dose of 1L Zintrac® ha−1 in the first season and second season, which corresponded to 78.97 and 57.86%. For the first season, increments were observed in all treatments that received zinc doses with increases of 48.70, 31.22, 14.30, and 15.93% for 1, 2, 3, and 4 L of Zintrac® ha−1 compared to the dose of 0 L ha−1. On the other hand, in the second season, there was an increase only in the dose of 1 L ha−1 of Zintrac®, which corresponded to 51.3% in the net yield (ha−1) of the dose of 0 L ha−1. Therefore, foliar zinc oxide fertilization was economically viable for the yam crop, obtaining higher economic indices at the dose of 1 L ha−1. The highest cost for growing yams is using a dose of 4 L ha−1 of Zintrac®, totaling USD 6977.59 (first season) and USD 6868.33 (second season) Full article
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12 pages, 998 KiB  
Article
Carbohydrate Ingestion before Exercise for Individuals with McArdle Disease: Survey Evidence of Implementation and Perception in Real-World Settings
by Sam L. Torrens, Evelyn B. Parr, Craig McNulty, Lynda Ross, Helen MacLaughlin and Robert A. Robergs
Nutrients 2024, 16(10), 1423; https://doi.org/10.3390/nu16101423 (registering DOI) - 9 May 2024
Abstract
In individuals with McArdle disease (IWMD), the ingestion of carbohydrates before exercise has previously been shown in laboratory studies to significantly decrease the exercising symptoms of the condition and increase exercise tolerance during the early stages of exercise. As a result, carbohydrate ingestion [...] Read more.
In individuals with McArdle disease (IWMD), the ingestion of carbohydrates before exercise has previously been shown in laboratory studies to significantly decrease the exercising symptoms of the condition and increase exercise tolerance during the early stages of exercise. As a result, carbohydrate ingestion pre-exercise is currently included in management guidelines, and often advised by medical professionals treating the condition. The aim of the current study was to determine whether positive lab-based results for the ingestion of carbohydrate before exercise in laboratory studies are being effectively translated into practice and produce perceptions of the same positive outcomes in real-world settings (RWS). An online survey method was used to collect responses from 108 IWMD. Data collected on the amount and type of carbohydrate consumed prior to exercise found that most surveyed participants (69.6%) who supplied qualitative data (n = 45) consumed less than the 37 g currently recommended in management guidelines. Survey data also revealed a large variation in the type and amount of carbohydrate ingested when IWMDs are applying carbohydrate ingestion before exercise in RWS. Consistent with these findings, only 17.5% of participants stated that they found carbohydrate ingestion before exercise relieved or minimised their MD symptoms. Results suggest that positive lab-based findings (increased exercise tolerance) of carbohydrate ingestion before exercise are not being effectively translated to RWS for many IWMD. There is a need for improved patient education of IWMD on the application of carbohydrate ingestion before exercise in RWS. Full article
(This article belongs to the Special Issue Diet and Nutrition: Metabolic Diseases)
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19 pages, 4183 KiB  
Article
Deciphering the Therapeutic Role of Lactate in Combating Disuse-Induced Muscle Atrophy: An NMR-Based Metabolomic Study in Mice
by Yu Zhou, Xi Liu, Zhen Qi, Longhe Yang, Caihua Huang and Donghai Lin
Molecules 2024, 29(10), 2216; https://doi.org/10.3390/molecules29102216 (registering DOI) - 9 May 2024
Abstract
Disuse muscle atrophy (DMA) is a significant healthcare challenge characterized by progressive loss of muscle mass and function resulting from prolonged inactivity. The development of effective strategies for muscle recovery is essential. In this study, we established a DMA mouse model through hindlimb [...] Read more.
Disuse muscle atrophy (DMA) is a significant healthcare challenge characterized by progressive loss of muscle mass and function resulting from prolonged inactivity. The development of effective strategies for muscle recovery is essential. In this study, we established a DMA mouse model through hindlimb suspension to evaluate the therapeutic potential of lactate in alleviating the detrimental effects on the gastrocnemius muscle. Using NMR-based metabolomic analysis, we investigated the metabolic changes in DMA-injured gastrocnemius muscles compared to controls and evaluated the beneficial effects of lactate treatment. Our results show that lactate significantly reduced muscle mass loss and improved muscle function by downregulating Murf1 expression, decreasing protein ubiquitination and hydrolysis, and increasing myosin heavy chain levels. Crucially, lactate corrected perturbations in four key metabolic pathways in the DMA gastrocnemius: the biosynthesis of phenylalanine, tyrosine, and tryptophan; phenylalanine metabolism; histidine metabolism; and arginine and proline metabolism. In addition to phenylalanine-related pathways, lactate also plays a role in regulating branched-chain amino acid metabolism and energy metabolism. Notably, lactate treatment normalized the levels of eight essential metabolites in DMA mice, underscoring its potential as a therapeutic agent against the consequences of prolonged inactivity and muscle wasting. This study not only advances our understanding of the therapeutic benefits of lactate but also provides a foundation for novel treatment approaches aimed at metabolic restoration and muscle recovery in conditions of muscle wasting. Full article
(This article belongs to the Section Medicinal Chemistry)
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16 pages, 3626 KiB  
Article
Potential of Subcritical Water Hydrolysis to Valorize Low-Valued Ray-Finned Fish (Labeobarbus nedgia): Effects of Hydrolysis Temperature and Pressurization Agent
by Solomon Abebaw Tadesse, Shimelis Admassu Emire, Pedro Barea, Alba Ester Illera, Rodrigo Melgosa, Sagrario Beltrán and María Teresa Sanz
Foods 2024, 13(10), 1462; https://doi.org/10.3390/foods13101462 (registering DOI) - 9 May 2024
Abstract
Subcritical water (SCW) hydrolysis was applied to valorize the low-valued ray-finned fish (Labeobarbus nedgia) into valuable protein hydrolysates, employing N2 and CO2 as pressurization agents at varying temperatures (140, 160, 180, and 200 °C). The degree of hydrolysis (DH) [...] Read more.
Subcritical water (SCW) hydrolysis was applied to valorize the low-valued ray-finned fish (Labeobarbus nedgia) into valuable protein hydrolysates, employing N2 and CO2 as pressurization agents at varying temperatures (140, 160, 180, and 200 °C). The degree of hydrolysis (DH) and total free amino acid content increased with temperature for both pressurizing agents. The highest DH (54.5 ± 0.4%) and total free amino acid content (210 ± 1 mg/gprot) were observed at 200 °C when CO2 gas was used as the pressurizing agent. Predominantly, glycine and alanine were released for both pressurizing agents. The antioxidant activity, evaluated through three different assays, increased with temperature and was found to be the highest at 200 °C. This study illustrated the advantages of the intensified SCW technology by using CO2 as a pressurization agent in valorizing low-valued ray-finned fish (Labeobarbus nedgia), as animal residue rich in proteins, for the production of valuable protein hydrolysates with a high fraction of valuable free amino acids, which could offer potential applications as a functional ingredient in the food industry. Full article
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18 pages, 533 KiB  
Article
Lactic Acid Bacteria and Bacillus subtilis as Potential Protective Cultures for Biopreservation in the Food Industry
by Nerea Garin-Murguialday, Laura Espina, Raquel Virto and Rafael Pagán
Appl. Sci. 2024, 14(10), 4016; https://doi.org/10.3390/app14104016 (registering DOI) - 9 May 2024
Abstract
The use of bacteria and/or their compounds is an alternative to the use of positive-list additives that the food industry is using as a tool to meet consumer demands for more natural, long-shelf-life, and healthy products, in short, to offer clean label foods. [...] Read more.
The use of bacteria and/or their compounds is an alternative to the use of positive-list additives that the food industry is using as a tool to meet consumer demands for more natural, long-shelf-life, and healthy products, in short, to offer clean label foods. The aim of this study is to investigate the suitability of cell-free supernatants (CFSs) from Qualified Presumption of Safety strains as bioprotective cultures. Out of an initial screening panel of about 200 isolates, strains Pediococcus acidilactici CNTA 1059, Lactiplantibacillus plantarum CNTA 600, Levilactobacillus brevis CNTA 1374, and Bacillus subtilis CNTA 517 demonstrated strong antimicrobial activity against, especially, Gram-positive bacteria. The CFSs of these four strains showed minimum inhibitory concentration values between 0.15% and 5% against Listeria monocytogenes and Lentilactobacillus parabuchneri. None of the four selected strains exhibited acquired resistance to target antibiotics, and the non-toxigenicity of all the CFSs was demonstrated. In the case of the three lactic acid bacteria, the presence of bacteriocin-like inhibitory substances was confirmed following the decline in antimicrobial activity due to treatment with proteases. Regarding B. subtilis, biosynthetic gene clusters for different bacteriocin-like substances, including protease-resistant lipoproteins, were found via whole-genome sequencing. In addition, all of the CFSs exhibited stable antimicrobial activity at a wide range of temperatures (70–121 °C) used for the pasteurization and sterilization of food products, with a loss of antimicrobial activity ranging from 3% to 28%. These results point to the possibility that CFSs from these strains could be used in the food industry as a biocontrol tool to develop new products. Full article
(This article belongs to the Special Issue Natural Products and Bioactive Compounds)
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14 pages, 7940 KiB  
Article
Tension and Shear Behaviour of Basalt Fiber Bio-Composites with Digital Image Correlation and Acoustic Emission Monitoring
by Tomaž Kek, Roman Šturm and Zoran Bergant
Polymers 2024, 16(10), 1331; https://doi.org/10.3390/polym16101331 (registering DOI) - 9 May 2024
Abstract
This research investigates the mechanical behavior and damage evolution in cross-ply basalt fiber composites subjected to different loading modes. A modified Arcan rig for simultaneous acoustic emission (AE) monitoring was designed and manufactured to apply quasi-isotropic shear, combined tensile and shear loading, and [...] Read more.
This research investigates the mechanical behavior and damage evolution in cross-ply basalt fiber composites subjected to different loading modes. A modified Arcan rig for simultaneous acoustic emission (AE) monitoring was designed and manufactured to apply quasi-isotropic shear, combined tensile and shear loading, and pure tensile loading on specimens with a central notch. Digital image correlation (DIC) was applied for high-resolution strain measurements. The measured failure strengths of the bio-composite specimens under different loading angles are presented. The different competing failure mechanisms that contribute to the local reduction in stress concentration are described. Different damage mechanisms trigger elastic waves in the composite, with distinct AE signatures that closely follow the sequence of fracture mechanisms. AE monitoring is employed to capture signals associated with structural damage initiation and progression. The characteristic parameters of AE signals are correlated with crack modes and damage mechanisms. The evolution of AE parameters during the peak load transition is presented, which enables the timely AE detection of the maximum load transition. The combination of DIC and AE monitoring improves understanding of the mechanical response and failure mechanisms in cross-ply basalt fiber composites, offering valuable insights for possible performance monitoring and structural reliability in diverse engineering applications. Full article
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22 pages, 10448 KiB  
Article
Changes in Forest Cover of Municipalities in Poland in 1990–2018
by Dagmara Kociuba, Leszek Gawrysiak and Waldemar Kociuba
Sustainability 2024, 16(10), 3954; https://doi.org/10.3390/su16103954 (registering DOI) - 9 May 2024
Abstract
The study is part of the trend of searching for research methods to demonstrate changes in forest cover at the level of basic units of public administration with greater precision and accuracy. The purpose of the article is to present, for the first [...] Read more.
The study is part of the trend of searching for research methods to demonstrate changes in forest cover at the level of basic units of public administration with greater precision and accuracy. The purpose of the article is to present, for the first time, changes in forest cover of municipalities in Poland from 1990 to 2018 using CORINE Land Cover (CLC) data. The contributions of this study are threefold. Firstly, using GIS and CLC data (3.1. Forests), multivariate analyses of forest cover changes were carried out for 2481 municipalities for the CLC data collection years (1990, 2000, 2006, 2012, 2018), which showed the temporal and spatial dynamics of changes, with a predominance of deforestation in 1990–2000 and 2012–2018, and afforestation in 2000–2006 and 2006–2012. Secondly, the formal, legal and financial rationale for these changes was indicated. The increase in afforestation was a result of financial incentives under the National Program for Increasing Forest Cover and the EU’s Common Agricultural Policy (under Rural Development Programs—RDPs). Deforestation was related to a decrease in the supply of land for afforestation, the competitiveness of subsidies implemented under RDPs, and statutory liberalization of logging. Thirdly, the main discrepancies between the data obtained from CLC and from the public data collected by Statistics Poland (GUS) and the State Forests were indicated, which ranged from −32 kha to +310 kha, corresponding to percentage differences of 2.3% and 1.8%, respectively. This was mainly influenced by the differences in the complexity and updating of data collected for state and private forests, as well as delays in introducing changes to the land register by the public administration. This work contributes significantly to our understanding of the dynamics of forest cover changes in relation to the actual degree of forestation and deforestation, and the determinants of forest transformation in Polish municipalities, as well as demonstrating the new applicability of CLC data and their limitations related mainly to the generalization of forest cover area. Full article
(This article belongs to the Section Sustainable Forestry)
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15 pages, 934 KiB  
Article
Informed Decision-Making with and for People with Dementia—Efficacy of the PRODECIDE Education Program for Legal Representatives: A Randomized Controlled Trial (PRODECIDE-RCT) and Process Evaluation
by Julia Lühnen, Burkhard Haastert and Tanja Richter
Geriatrics 2024, 9(3), 60; https://doi.org/10.3390/geriatrics9030060 (registering DOI) - 9 May 2024
Abstract
Legal representatives take a major role in healthcare decisions with and for people with dementia, but only a minority has a qualification in this field. The aim was to evaluate the efficacy of the PRODECIDE education program for legal representatives. In a prospective [...] Read more.
Legal representatives take a major role in healthcare decisions with and for people with dementia, but only a minority has a qualification in this field. The aim was to evaluate the efficacy of the PRODECIDE education program for legal representatives. In a prospective randomized controlled trial, legal representatives (volunteers and professionals, representing at least one person with dementia) were allocated (1:1 computer-generated block randomization) to the intervention (PRODECIDE education program) and control (standard care) groups. The primary outcome measure was knowledge, operationalized as the understanding of decision-making processes and in setting realistic expectations. Only data entry and analyses were blinded. A process evaluation in a mixed methods design was performed. We enrolled 218 legal representatives, and 216 were included in the primary analysis (intervention n = 109, control n = 107). The percentage of correct answers in the knowledge test post intervention was 69.0% in the intervention and 43.4% in the control group (difference 25.6%; CI 95%, 21.3 to 29.8; p < 0.001). In the comparison of professional and voluntary representatives, professionals had 13.6% (CI 95%, 8.0 to 19.2; p < 0.001) more correct answers. The PRODECIDE education program can improve the knowledge of legal representatives, an important prerequisite for evidence-based, informed decision-making. Full article
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21 pages, 6387 KiB  
Article
Genistein–Aspirin Combination Exerts Cytotoxic and Anti-Migratory Effects in Human Colorectal Cancer Cells
by Claudia Iftode, Stela Iurciuc, Iasmina Marcovici, Ioana Macasoi, Dorina Coricovac, Cristina Dehelean, Sorin Ursoniu, Andreea Rusu and Simona Ardelean
Life 2024, 14(5), 606; https://doi.org/10.3390/life14050606 (registering DOI) - 9 May 2024
Abstract
Colorectal cancer (CRC) is a heterogenous pathology with high incidence and mortality rates globally, but it is also preventable so finding the most promising candidates (natural compounds or repurposed drugs) to be chemopreventive alternatives has become a topic of interest in recent years. [...] Read more.
Colorectal cancer (CRC) is a heterogenous pathology with high incidence and mortality rates globally, but it is also preventable so finding the most promising candidates (natural compounds or repurposed drugs) to be chemopreventive alternatives has become a topic of interest in recent years. The present work aims to elucidate the potential effects of a combination between genistein (GEN), an isoflavone of natural origin, and aspirin (ASA) in CRC prevention/treatment by performing an in vitro evaluation in human colorectal cancer cells (HCT-116) and an in ovo analysis using the chick embryo chorioallantoic membrane (CAM) model. Cell viability was verified by an MTT (migratory potential by scratch) assay, and the expressions of MMP-2 and MMP-9 were analyzed using RT-qPCR. Our results indicated a dose-dependent cytotoxic effect of ASA (2.5 mM) + GEN (10–75 µM) combination characterized by reduced cell viability and morphological changes (actin skeleton reorganization and nuclei deterioration), an inhibition of HCT-116 cells’ migratory potential by down-regulating MMP-2 and MMP-9 mRNA expressions, and an antiangiogenic effect by modifying the vascular network. These promising results raise the possibility of future in-depth investigations regarding the chemopreventive/therapeutical potential of ASA+GEN combination. Full article
(This article belongs to the Special Issue Natural Bioactives: Exploring Their Therapeutic Potential)
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17 pages, 4991 KiB  
Article
MOF-Derived ZrO2-Supported Bimetallic Pd–Ni Catalyst for Selective Hydrogenation of 1,3-Butadiene
by Ying Liu, Lili Liu, Leyuan Wang, Miaoliang Zang, Lei Li and Yunkai Zhang
Molecules 2024, 29(10), 2217; https://doi.org/10.3390/molecules29102217 (registering DOI) - 9 May 2024
Abstract
A series of MOF-derived ZrO2-supported Pd-Ni bimetallic catalysts (PdNi/UiO-67-CTAB(n)-A500) were prepared by co-impregnation and pyrolysis at 500 °C under air atmosphere using UiO-67-CTAB(n) (CTAB: cetyltrimethylammonium bromide; n: the concentration of CTAB; n = 0, 3, 8, 13, 18) as a sacrificial [...] Read more.
A series of MOF-derived ZrO2-supported Pd-Ni bimetallic catalysts (PdNi/UiO-67-CTAB(n)-A500) were prepared by co-impregnation and pyrolysis at 500 °C under air atmosphere using UiO-67-CTAB(n) (CTAB: cetyltrimethylammonium bromide; n: the concentration of CTAB; n = 0, 3, 8, 13, 18) as a sacrificial template. The catalytic activity of PdNi/UiO-66-CTAB(n)-A500 in 1,3-butadiene hydrogenation was found to be dependent on the crystal morphology of the UiO-67 template. The highest activity was observed over the PdNi/UiO-67-CTAB(3)-A500 catalyst which was synthesized using UiO-67-CTAB(3) with uniform octahedral morphology as the template for the 1,3-butadiene selective hydrogenation. The 1,3-butadiene conversion and total butene selectivity were 98.4% and 44.8% at 40 °C within 1 h for the PdNi/UiO-67-CTAB(3)-A500 catalyst, respectively. The catalyst of PdNi/UiO-67-CTAB(3)-A500 can be regenerated in flowing N2 at 200 °C. Carbon deposited on the surface of the catalyst was the main reason for its deactivation. This work is valuable for the high-efficiency bimetallic catalyst’s development on the selective hydrogenation of 1,3-butadiene. Full article
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15 pages, 652 KiB  
Article
Sustainable Sheep and Goat Farming in Arid Regions of Jordan
by Faisal Al-Barakeh, Ashraf Omar Khashroum, Radi A. Tarawneh, Fatima A. Al-Lataifeh, Azzam N. Al-Yacoub, Moammar Dayoub and Khaled Al-Najjar
Ruminants 2024, 4(2), 241-255; https://doi.org/10.3390/ruminants4020017 (registering DOI) - 9 May 2024
Abstract
This study examines the sustainability of livestock farming in Jordan’s arid regions, focusing on smallholder Awassi sheep and Baladi goat farming. It assesses breeders, economic factors, herd productivity, and sustainability, evaluating the vitality of these breeds by examining reproductive success through birth and [...] Read more.
This study examines the sustainability of livestock farming in Jordan’s arid regions, focusing on smallholder Awassi sheep and Baladi goat farming. It assesses breeders, economic factors, herd productivity, and sustainability, evaluating the vitality of these breeds by examining reproductive success through birth and survival rates. In 2021–2022, a total of 53 traditional Awassi sheep and Baladi goat farms in Northern Jordan’s Mafraq Governorate were surveyed using a comprehensive questionnaire to gather data on breeders’ information, farm economics, and herd indicators. Dry seasons in arid lands are tough on sheep and goats, with limited fodder and water availability harming their health and reproductive capacity. While wet seasons bring relief, they can also introduce diseases. Special care for these animals throughout the year is crucial to maintain healthy herds in arid regions. Linear models were employed to analyze sample data, with variance and correlation tests used to explore relationships among variables. Additionally, chi-squared analysis was utilized to assess the impact of male replacement plans on these herds. The main outcomes of this study reveal that breeder indicators pose challenges to the sustainability and growth of Awassi sheep and Baladi goat farming. Conversely, economic indicators suggest a blend of financial stability alongside obstacles related to profitability and resource ownership. Breeder age emerges as a significant factor in livestock herd management, influenced by elements such as experience, resource availability, and adherence to traditional practices. Experience notably influences breeders’ decisions regarding male insemination replacements, likely due to acquired skills. Crop cultivation is shown to reduce fodder costs, thereby enhancing profitability for each young animal sold and consequently increasing the breeders’ returns. Winter conditions elevate animal mortality rates due to cold stress, while other animals within herds contribute to increased kid mortality by facilitating disease transmission. Breeder experience significantly impacts lamb survival, underscoring its importance in ensuring proper care. Increasing ewe proportions within herds enhances lamb production and lowers lamb mortality rates. Effective ram management and yearling lamb sales further contribute to herd health and productivity. A higher proportion of goats within a herd positively correlates with the number of young yearlings sold but inversely affects breeding males and overall kid numbers, indicating a delicate balance that influences herd productivity and mortality rates. Sheep (92.5%) and goat herds (90.6%) demonstrate reproductive success, indicative of superior long-term vitality. While sheep excel in birth rates, survival, and sales, they exhibit lower ewe and lambing percentages. Goat farming demonstrates high birth rates, sales, and overall success but requires particular attention to ensuring kid survival, especially during the winter months. Improving the characteristics of the Awassi sheep and Baladi goat flocks is crucial for ensuring sustainability, achievable through proper care practices in dry lands. In conclusion, despite the challenges, there are promising opportunities to enhance sheep and goat farming in Jordan. Effective management, informed by the breeders’ experience and economics, is crucial. Empowering small-scale herders and implementing targeted interventions, like winter mortality solutions and selective breeding, is vital for sustainable growth. Full article
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13 pages, 1034 KiB  
Article
Modeling an Investment Framework for BMTA Electric Bus Fleet Development
by Sorawit Wanitanukul, Kuskana Kubaha and Roongrojana Songprakorp
World Electr. Veh. J. 2024, 15(5), 206; https://doi.org/10.3390/wevj15050206 (registering DOI) - 9 May 2024
Abstract
In Thailand, diesel buses are notorious for their poor energy efficiency and contribution to air pollution. To combat these issues, battery electric buses (BEBs) have emerged as a promising alternative. However, their high initial costs have posed challenges for fleet management, especially for [...] Read more.
In Thailand, diesel buses are notorious for their poor energy efficiency and contribution to air pollution. To combat these issues, battery electric buses (BEBs) have emerged as a promising alternative. However, their high initial costs have posed challenges for fleet management, especially for agencies such as the Bangkok Mass Transit Authority (BMTA). This study aims to revolutionize BEB fleet management by developing an energy model tailored to the BMTA’s needs. The methodology consists of two crucial steps: analyzing BMTA bus routes and designing fleet management and charging systems. Through this process, the study seeks to determine the maximum number of BEBs that can be operated on each route with the fewest chargers possible. The results reveal exciting possibilities. Within the city bus landscape, two out of five BMTA bus routes show potential for transitioning to BEBs, provided they meet a maximum energy requirement of 200 kWh every two rounds. This analysis identifies routes ripe for BEB adoption while considering the limitations of battery size. In the next step, the study unveils a game-changing strategy: a maximum of 13 BEBs can operate on two routes with just four chargers requiring 150 kW each. This means fewer chargers and more efficient operations. Plus, the charging profile peaks at 600 kW from 4:00 to 8:00 p.m., showing when and where the fleet needs power the most. However, the real eye-opener? Significant energy savings of THB 10.44 million per year compared to diesel buses, with an initial investment cost savings of over 37%. These findings underscore the potential for BEB fleet management to revolutionize public transportation and save money in the long run. However, there is more work to be done. The study highlights the need for real-time passenger considerations, the development of post-service charging strategies, and a deeper dive into total lifetime costs. These areas of improvement promise even greater strides in the future of sustainable urban transportation. Full article
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