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11 pages, 810 KiB

Open AccessArticle

Vaccine Acceptance in Patients with Inflammatory Bowel Disease: Lessons Learned from the COVID-19 Pandemic

by Giada Mastrangeli, Filippo Vernia, Stefano Necozione, Mario Muselli, Sara Frassino, Nicola Cesaro, Giovanni Latella and Leila Fabiani

Vaccines 2024, 12(5), 551; https://doi.org/10.3390/vaccines12050551 (registering DOI) - 18 May 2024

Abstract

Background: Immunomodulating therapies, which are commonly used in patients with Crohn’s disease (CD) and ulcerative colitis (UC), have been linked to an increased risk of contracting opportunistic infectious diseases, the majority of which are preventable through vaccination. Nonetheless, vaccination rates in these patients [...] Read more.

Background: Immunomodulating therapies, which are commonly used in patients with Crohn’s disease (CD) and ulcerative colitis (UC), have been linked to an increased risk of contracting opportunistic infectious diseases, the majority of which are preventable through vaccination. Nonetheless, vaccination rates in these patients are suboptimal, and frequently lower than in the general population. The COVID-19 immunization schedule provided a new scenario for investigating vaccine acceptance in patients with inflammatory bowel disease (IBD), with uncertainty and concerns emerging and the number of subjects receiving the third and fourth doses of the vaccine gradually decreasing. This study investigated IBD patients’ attitudes towards previous COVID-19 vaccine programs and identified the factors that influence their adherence. It considered demographic and disease-related factors as well as the role of gastroenterologists and primary care physicians (PCPs). Methods: Data were collected through a self-completed questionnaire administered to all adult IBD patients (age > 18) who visited the Gastroenterology, Hepatology, and Nutrition division at the University of L’Aquila (Italy) for a regular follow-up between November 2021 and December 2022. Non-IBD gastroenterological outpatients who visited during the same period were included as a control group. Results: A total of 178 patients were included in the analysis. The IBD group consisted of 77 patients, 48.1% with CD and 51.9% with UC; the mean age was 49.5 years and 51.9% were female. Overall, 94.8% of IBD patients had undergone at least one vaccine dose and 79.2% had received two doses, versus 8% of the control group (p < 0.0001). A total of 84.4% of IBD patients reported their propensity towards COVID-19 vaccination, with an average agreement score significantly higher than the controls (p = 0.0044). The trust of IBD patients in the effectiveness of the COVID-19 vaccine (p < 0.0001) and its role in hastening pandemic resolution (p < 0.0001) is strongly related to motivation and propensity. Concerns about the safety of the COVID-19 vaccine in IBD (p = 0.0202) and fear of vaccine-induced flare-ups (p = 0.0192) were reported as the main barriers. No correlation was found between COVID-19 vaccine propensity and clinical features like the type of IBD, years of disease, activity, and ongoing treatment. Regarding the recommendations received from physicians to get vaccinated against COVID-19, IBD patients relied heavily on their gastroenterologists for advice, while the control group relied mainly on their PCPs. Conclusions: The overall positive attitude towards vaccinations reported in our study was better than that observed for other vaccines. The relationship of trust with the gastroenterologist should be used to boost vaccination against other preventable diseases in IBD patients. Our findings add information on the factors influencing vaccine propensity, which can be used to improve current vaccination strategies. Full article

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11 pages, 568 KiB

Open AccessArticle

Outcomes of Budesonide as a Treatment Option for Immune Checkpoint Inhibitor-Related Colitis in Patients with Cancer

by Antonio Pizuorno Machado, Abdullah Salim Shaikh, Alice Saji, Malek Shatila, Isabella Glitza Oliva, Yinghong Wang and Anusha Shirwaikar Thomas

Cancers 2024, 16(10), 1919; https://doi.org/10.3390/cancers16101919 (registering DOI) - 18 May 2024

Abstract

Background: Current treatment guidelines for moderate to severe colitis (IMC) secondary to immune checkpoint inhibitors (ICI) recommend systemic corticosteroids as the primary therapy in conjunction with biologics, namely infliximab and/or vedolizumab. We aimed to explore the efficacy and safety of oral budesonide in [...] Read more.

Background: Current treatment guidelines for moderate to severe colitis (IMC) secondary to immune checkpoint inhibitors (ICI) recommend systemic corticosteroids as the primary therapy in conjunction with biologics, namely infliximab and/or vedolizumab. We aimed to explore the efficacy and safety of oral budesonide in the treatment of IMC. Methods: We performed a retrospective analysis at MD Anderson Cancer Center of adult cancer patients with a confirmed (based on clinical, radiographic and laboratory assessment) diagnosis of IMC between 1 January 2015 and 31 November 2022, treated with budesonide. Data collection included demographics, oncologic history, IMC-related information and outcomes up to 6 months after the last dose of ICI. Results: Our sample (n = 69) comprised primarily of Caucasian (76.8%) females (55.1%). The majority of patients received combination therapy with anti-PD-1/L1 and anti-CTLA-4 (49.3%), and the most common malignancy treated was melanoma (37.6%). The median grade of diarrhea was 3 and of colitis was 2. Of the 50 patients who underwent endoscopic evaluation, a majority had non-ulcerative inflammation (64%) and active colitis on histology (78%). Budesonide was used as primary treatment at onset of IMC in 56.5% patients, as well as a bridging therapy from systemic corticosteroids in 33.3%. Less than half of the patients (44.9%) required additional therapies such as biologics or fecal microbiota transplant. Additionally, 75.3% of patients achieved full remission of IMC and 24.6% had a recurrence of IMC. ICI was resumed in 31.9% of patients and 17.4% received other forms of cancer therapies. Conclusions: Budesonide may be an effective strategy to treat and prevent the recurrence of IMC. The remission rates observed in our analysis with budesonide alone are comparable to systemic corticosteroids. Patients that require an extended duration of steroid exposure and those with moderate to severe colitis may benefit from budesonide given its lower risk of infection and complications. Furthermore, we observe that budesonide may serve as a successful bridge from systemic corticosteroids with subsequent biologic treatment. Larger prospective studies are necessary to determine the role of budesonide as well as its safety profile. Full article

(This article belongs to the Section Cancer Therapy)

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13 pages, 5057 KiB

Open AccessArticle

Utilization of Charcoal Obtained from Woody Biomass in Metallurgical Processes Based on Solid–Gas Reactions

by Christian Dornig, Gustav Hanke and Jürgen Antrekowitsch

Metals 2024, 14(5), 592; https://doi.org/10.3390/met14050592 (registering DOI) - 18 May 2024

Abstract

The high demand for carbon-based products within pyrometallurgy is placing the industry in an increasingly challenging position to meet stringent requirements. To transition away from fossil carbon carriers, biochar emerges as a sustainable and CO₂-neutral alternative, presenting a viable solution without [...] Read more.

The high demand for carbon-based products within pyrometallurgy is placing the industry in an increasingly challenging position to meet stringent requirements. To transition away from fossil carbon carriers, biochar emerges as a sustainable and CO₂-neutral alternative, presenting a viable solution without necessitating fundamental adjustments to plant technology, unlike hydrogen as an alternative reducing agent. Prior investigations have underscored the potential of woody biomass pyrolysis products for CO₂-neutral metallurgy. Nonetheless, it is imperative to recognize that biochar must meet distinct requirements across various metallurgical processes. This paper conducts a comparative analysis between biochar and petroleum coke using thermogravimetric analyses, surface measurements, reactivity assessments, and scanning electron microscopy. Furthermore, the performance in a furnace for simulating the Waelz process, specifically regarding ZnO reduction, is scrutinized. The results illustrate the optical differences between petroleum coke and biochar and the significantly higher reactivity and specific surface area of biochar. When used in solid–gas reactors, it is observed that due to its high reactivity, biochar reacts more vigorously and carbon is completely consumed. However, during the reduction of ZnO, only minor differences were monitored, making biochar comparable to petroleum coke. Therefore, under certain constraints, biochar can be considered a potential substitute for metallurgical solid–gas reactions. Full article

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15 pages, 4539 KiB

Open AccessArticle

Exploring a New Physical Scenario of Virtual Water Molecules in the Application of Measuring Virtual Trees Using Computational Virtual Measurement

by Zhichao Wang, Xiaoning Zhang, Xiaoyuan Zhang, Xinli Pan, Tiantian Ma, Zhongke Feng and Christiane Schmullius

Forests 2024, 15(5), 880; https://doi.org/10.3390/f15050880 (registering DOI) - 18 May 2024

Abstract

Our previous studies discussed the potential of measuring virtual trees using computational virtual measurement (CVM). CVM is a general methodology that employs observational techniques in lieu of mathematical processing. The advantage of CVM lies in its ability to circumvent mathematical assumptions of tree [...] Read more.

Our previous studies discussed the potential of measuring virtual trees using computational virtual measurement (CVM). CVM is a general methodology that employs observational techniques in lieu of mathematical processing. The advantage of CVM lies in its ability to circumvent mathematical assumptions of tree shapes at the algorithmic level. However, due to the current computational limitations of desktop computers, the previously developed CVM application, namely, virtual water displacement (VWD), could only act as a primary theoretical testimonial using an idealized point cloud of a tree. The key problem was that simulating a massive number of virtual water molecules (VMMs) consumed most of the computational resources. As a consequence, an unexpected empirical formula for volume calibration had to be applied to the output measurement results. Aiming to create a more realistic simulation of what occurs when water displacement is used to measure tree volume in the real world, in this study, we developed a new physical scenario for VWMs. This new scenario, namely, a flood area mechanism (FAM), employed footprints of VWMs instead of quantifying VWM counts. Under a FAM, the number of VMMs was reduced to a few from several thousands, making the empirical mathematical process (of the previously developed physical scenario of VWMs) unnecessary. For the same ideal point clouds as those used in our previous studies, the average volume overestimations were found to be 6.29% and 2.26% for three regular objects and two artificial stems, respectively. Consequently, we contend that FAM represents a closer approximation to actual water displacement methods for measuring tree volume in nature. Therefore, we anticipate that the VWD method will eventually utilize the complete tree point cloud with future advancements in computing power. It is necessary to develop methods such as VWD and more CVM applications for future applications starting now. Full article

(This article belongs to the Special Issue Integrated Measurements for Precision Forestry)

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4 pages, 186 KiB

Open AccessEditorial

Editorial for the Special Issue Titled “Design of Polymeric Hydrogels Biomaterials”

by Ana Paula Serro, Maria Vivero-Lopez and Diana C. Silva

Gels 2024, 10(5), 344; https://doi.org/10.3390/gels10050344 (registering DOI) - 18 May 2024

Abstract

Hydrogels have attracted great interest in the biomedical applications field in recent years [...] Full article

(This article belongs to the Special Issue Design of Polymeric Hydrogels Biomaterials)

21 pages, 480 KiB

Open AccessArticle

Reliability and Exploratory Factor Analysis of a Measure of the Psychological Distance from Climate Change

by Alan E. Stewart

Climate 2024, 12(5), 76; https://doi.org/10.3390/cli12050076 (registering DOI) - 18 May 2024

Abstract

Psychological distance from climate change has emerged as an important construct in understanding sustainable behavior and attempts to mitigate and/or adapt to climate change. Yet, few measures exist to assess this construct and little is known about the properties of the existing measures. [...] Read more.

Psychological distance from climate change has emerged as an important construct in understanding sustainable behavior and attempts to mitigate and/or adapt to climate change. Yet, few measures exist to assess this construct and little is known about the properties of the existing measures. In this article, the author conducted two studies of a psychological distance measure developed by Wang and her colleagues. In Study 1, the author assessed the test–retest reliability of the measure over a two-week interval and found the scores to be acceptably stable over time. In Study 2, the author conducted two exploratory factor analyses, using different approaches to the correlation and factor extraction. Similar results were observed for each factor analysis: one factor was related to items that specified greater psychological distance from climate change; a second factor involved items that specified closeness to climate change; and a third involved the geographic/spatial distance from climate change. The author discussed the results and provided recommendations on ways that the measure may be used to research the construct of psychological distance from climate change. Full article

(This article belongs to the Special Issue Anthropogenic Climate Change: Social Science Perspectives - Volume II)

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18 pages, 4546 KiB

Open AccessArticle

Facial Skin Microbiome Composition and Functional Shift with Aging

by Allison Garlet, Valerie Andre-Frei, Nicolas Del Bene, Hunter James Cameron, Anita Samuga, Vimal Rawat, Philipp Ternes and Sabrina Leoty-Okombi

Microorganisms 2024, 12(5), 1021; https://doi.org/10.3390/microorganisms12051021 (registering DOI) - 18 May 2024

Abstract

The change in the skin microbiome as individuals age is only partially known. To provide a better understanding of the impact of aging, whole-genome sequencing analysis was performed on facial skin swabs of 100 healthy female Caucasian volunteers grouped by age and wrinkle [...] Read more.

The change in the skin microbiome as individuals age is only partially known. To provide a better understanding of the impact of aging, whole-genome sequencing analysis was performed on facial skin swabs of 100 healthy female Caucasian volunteers grouped by age and wrinkle grade. Volunteers’ metadata were collected through questionnaires and non-invasive biophysical measurements. A simple model and a biological statistical model were used to show the difference in skin microbiota composition between the two age groups. Taxonomic and non-metric multidimensional scaling analysis showed that the skin microbiome was more diverse in the older group (≥55 yo). There was also a significant decrease in Actinobacteria, namely in Cutibacterium acnes, and an increase in Corynebacterium kroppenstedtii. Some Streptococcus and Staphylococcus species belonging to the Firmicutes phylum and species belonging to the Proteobacteria phylum increased. In the 18–35 yo younger group, the microbiome was characterized by a significantly higher proportion of Cutibacterium acnes and Lactobacillus, most strikingly, Lactobacillus crispatus. The functional analysis using GO terms revealed that the young group has a higher significant expression of genes involved in biological and metabolic processes and in innate skin microbiome protection. The better comprehension of age-related impacts observed will later support the investigation of skin microbiome implications in antiaging protection. Full article

(This article belongs to the Special Issue Microbiota in Human Health and Disease)

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11 pages, 4425 KiB

Open AccessArticle

Boosting the Anti-Infection Ability of Titanium Implants by Coating Polydopamine–Curcumin

by Manlong Chen, Wenyi Yu, Qi Shi, Han Wen, Guojing Li, Yunliang Wang, Tao Wang, Shibin Liu and Tingting Yang

Coatings 2024, 14(5), 640; https://doi.org/10.3390/coatings14050640 (registering DOI) - 18 May 2024

Abstract

To reduce the risk of infection, improving the anti-infection ability of Ti-based implantation has become a very meaningful task. In this work, by employing polydopamine (PDA) as a carrier and curcumin (CUR) as an anti-biotic/inflammatory, a series of Ti-PDA@CURx (x = 0.5, 1.0, [...] Read more.

To reduce the risk of infection, improving the anti-infection ability of Ti-based implantation has become a very meaningful task. In this work, by employing polydopamine (PDA) as a carrier and curcumin (CUR) as an anti-biotic/inflammatory, a series of Ti-PDA@CURx (x = 0.5, 1.0, 1.5, 2.0, 2.5) was successfully fabricated and characterized by scanning electron microscopy, Fourier transform infrared spectrometry, X-ray diffraction, X-ray photoelectron spectroscopy, and the water contact angle, where Ti plates were firmly coated by PDA@CUR. The test result of CUR content shows that the maximum loading of CUR in PDA can reach 0.6506%, where the CUR concentration is 1.5 mg/mL. The antibacterial test results demonstrate that Ti-PDA@CUR-x (x = 0.5, 1.0, 1.5, 2.0, 2.5) exhibit significant antibacterial activities against Escherichia coli and S. aureu, where [email protected] has the highest antibacterial rate of 62.7% against Escherichia coli and 52.6% against S. aureus. The cytotoxicity test shows that Ti-PDA, Ti-PDA@CUR-x (x = 0.5, 1.0, 1.5, 2.0, 2.5) has almost no toxicity. Full article

(This article belongs to the Section Bioactive Coatings and Biointerfaces)

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13 pages, 4966 KiB

Open AccessSystematic Review

H3^K27-Altered Diffuse Glioma of the Spinal Cord in Adult Patients: A Qualitative Systematic Review and Peculiarity of Radiological Findings

by Anna Maria Auricchio, Giovanni Pennisi, Grazia Menna, Alessandro Olivi, Marco Gessi, Gerrit H. Gielen, Simona Gaudino, Nicola Montano and Fabio Papacci

J. Clin. Med. 2024, 13(10), 2972; https://doi.org/10.3390/jcm13102972 (registering DOI) - 18 May 2024

Abstract

Background: Primary spinal cord diffuse gliomas (SpDG) are rare tumors that may harbor, like diffuse intrinsic pontine gliomas (DIPG), H3^K27M mutations. According to the WHO (2021), SpDGs are included in diffuse midline H3K27-altered gliomas, which occur more frequently in adults and show [...] Read more.

Background: Primary spinal cord diffuse gliomas (SpDG) are rare tumors that may harbor, like diffuse intrinsic pontine gliomas (DIPG), H3^K27M mutations. According to the WHO (2021), SpDGs are included in diffuse midline H3K27-altered gliomas, which occur more frequently in adults and show unusual clinical presentation, neuroradiological features, and clinical behavior, which differ from H3 G34-mutant diffuse hemispheric glioma. Currently, homogeneous adult-only case series of SpDG, with complete data and adequate follow-up, are still lacking. Methods: We conducted a qualitative systematic review, focusing exclusively on adult and young adult patients, encompassing all studies reporting cases of primitive, non-metastatic SpDG with H3^K27 mutation. We analyzed the type of treatment administered, survival, follow-up duration, and outcomes. Results: We identified 30 eligible articles published between 1990 and 2023, which collectively reported on 62 adult and young adult patients with primitive SpDG. Postoperative outcomes were assessed based on the duration of follow-up, with outcomes categorized as either survival or mortality. Patients who underwent surgery were followed up for a mean duration of 17.37 months, while those who underwent biopsy had a mean follow-up period of 14.65 months. Among patients who were still alive, the mean follow-up duration was 18.77 months. The radiological presentation of SpDG varies widely, indicating its lack of uniformity. Conclusion: Therefore, we presented a descriptive scenario where SpDG was initially suspected to be a meningioma, but was later revealed to be a malignant SpDG with H3^K27M mutation. Full article

(This article belongs to the Special Issue Targeted Diagnosis and Treatment in Lumbar and Spine Surgeries)

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17 pages, 2591 KiB

Open AccessArticle

Decoding the Effects of High Hydrostatic Pressure and High-Temperature Short-Time Sterilization on the Volatile Aroma Profile of Red Raspberry Juice

by Wentao Zhang, Xuejie Li, Xuzeng Wang, He Li, Xiaojun Liao, Fei Lao, Jihong Wu and Jian Li

Foods 2024, 13(10), 1574; https://doi.org/10.3390/foods13101574 (registering DOI) - 18 May 2024

Abstract

The loss of distinctive aromas due to sterilization significantly hinders efforts to enhance the sensory quality of fruit and vegetable juices. This study aimed to elucidate the impacts of high-hydrostatic pressure (HHP) and high-temperature short-time (HTST) sterilization methods on the loss of C6 [...] Read more.

The loss of distinctive aromas due to sterilization significantly hinders efforts to enhance the sensory quality of fruit and vegetable juices. This study aimed to elucidate the impacts of high-hydrostatic pressure (HHP) and high-temperature short-time (HTST) sterilization methods on the loss of C6 aldehyde aroma-active compounds in red raspberry juice. External standard quantification and quantitative descriptive analysis (QDA) revealed a notable decline in the levels of hexanal and (Z)-3-hexenal following the HHP and HTST treatments (p < 0.05), resulting in a marked attenuation of the grassy aroma characteristic of red raspberry juice. Furthermore, a comprehensive examination of the precursors, pivotal enzymes, intermediates, and downstream aromas within the fatty acid metabolism pathway in different raspberry juice samples indicated that the C6 aldehydes loss induced by HHP and HTST sterilizations was primarily ascribed to the competitive inhibition of β-oxidation and the hindered enzymatic oxidation of fatty acids. These insights suggest that modifying sterilization protocols and enhancing enzymatic stability may help preserve the aroma integrity of raspberry juice. Our findings offer practical guidance for optimizing juice processing techniques to maintain flavor. Full article

(This article belongs to the Section Drinks and Liquid Nutrition)

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15 pages, 669 KiB

Open AccessReview

Genetics of Hypertension: From Monogenic Analysis to GETomics

by Martina Zappa, Michele Golino, Paolo Verdecchia and Fabio Angeli

J. Cardiovasc. Dev. Dis. 2024, 11(5), 154; https://doi.org/10.3390/jcdd11050154 (registering DOI) - 18 May 2024

Abstract

Arterial hypertension is the most frequent cardiovascular risk factor all over the world, and it is one of the leading drivers of the risk of cardiovascular events and death. It is a complex trait influenced by heritable and environmental factors. To date, the [...] Read more.

Arterial hypertension is the most frequent cardiovascular risk factor all over the world, and it is one of the leading drivers of the risk of cardiovascular events and death. It is a complex trait influenced by heritable and environmental factors. To date, the World Health Organization estimates that 1.28 billion adults aged 30–79 years worldwide have arterial hypertension (defined by European guidelines as office systolic blood pressure ≥ 140 mmHg or office diastolic blood pressure ≥ 90 mmHg), and 7.1 million die from this disease. The molecular genetic basis of primary arterial hypertension is the subject of intense research and has recently yielded remarkable progress. In this review, we will discuss the genetics of arterial hypertension. Recent studies have identified over 900 independent loci associated with blood pressure regulation across the genome. Comprehending these mechanisms not only could shed light on the pathogenesis of the disease but also hold the potential for assessing the risk of developing arterial hypertension in the future. In addition, these findings may pave the way for novel drug development and personalized therapeutic strategies. Full article

(This article belongs to the Special Issue Advances in the Diagnosis, Treatment, and Risk Stratification of Hypertension)

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13 pages, 3416 KiB

Open AccessArticle

Long-Term Clinical Outcomes of 3D-Printed Subperiosteal Titanium Implants: A 6-Year Follow-Up

by Neculai Onică, Dana Gabriela Budală, Elena-Raluca Baciu, Cezara Andreea Onică, Gabriela Luminița Gelețu, Alice Murariu, Mihail Balan, Mihaela Pertea and Carmen Stelea

J. Pers. Med. 2024, 14(5), 541; https://doi.org/10.3390/jpm14050541 (registering DOI) - 18 May 2024

Abstract

As an alternative to regenerative therapies, numerous authors have recently proposed bringing back subperiosteal implants. The aim of the study was to present our clinical experience with a subperiosteal jaw implant that needs minimal bone preparation and enables the rapid implantation of prosthetic [...] Read more.

As an alternative to regenerative therapies, numerous authors have recently proposed bringing back subperiosteal implants. The aim of the study was to present our clinical experience with a subperiosteal jaw implant that needs minimal bone preparation and enables the rapid implantation of prosthetic teeth in edentulous, atrophic alveolar bone. The research included 36 complete or partial edentulous patients (61 subperiostal implants) over a period of 6 years. To create the patient-specific subperiostal implants design, DentalCAD 3.0 Galway software (exocad GmbH, Darmstadt, Germany) was used and fabricated with a Mysint 100 (Sisma S.p.A., Piovene Rocchette, Italy) by titanium alloy powder. The results showed that only 9 of the 36 cases were successful at 6-year follow-up, while 27 cases had complications, including exposure of the metal frame (early or delayed), mobility of the device prior to the first 4–6 months, and late mobility due to recurrent infections and progressive structure exposure; 1 case failed for reasons unrelated to the device. This study indicated that the prudent application of fully customized subperiosteal jaw implants is a dependable alternative for the dental rehabilitation of atrophic edentulous cases that necessitate bone grafts for traditional fixed dental implant solutions. Full article

(This article belongs to the Special Issue New Updates in Oral and Maxillofacial Surgery)

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11 pages, 2270 KiB

Open AccessArticle

Competitive Mechanism of Alloying Elements on the Physical Properties of Al₁₀Ti₁₅Ni_x₁Cr_x₂Co_x₃ Alloys through Single-Element and Multi-Element Analysis Methods

by Yu Liu, Lijun Wang, Juangang Zhao, Zhipeng Wang, Ruizhi Zhang, Yuanzhi Wu, Touwen Fan and Pingying Tang

Coatings 2024, 14(5), 639; https://doi.org/10.3390/coatings14050639 (registering DOI) - 18 May 2024

Abstract

Altering the content of an alloying element in alloy materials will inevitably affect the content of other elements, while the effect is frequently disregarded, leading to subsequent negligence of the common influence on the physical properties of alloys. Therefore, the correlation between alloying [...] Read more.

Altering the content of an alloying element in alloy materials will inevitably affect the content of other elements, while the effect is frequently disregarded, leading to subsequent negligence of the common influence on the physical properties of alloys. Therefore, the correlation between alloying elements and physical properties has not been adequately addressed in the existing studies. In response to this problem, the present study focuses on the Al₁₀Ti₁₅Ni_x₁Cr_x₂Co_x₃ alloys and investigates the competitive interplay among Ni, Cr, and Co elements in the formation of physical properties through a single-element (SE) analysis and a multi-element (ME) analysis based on the first principles calculations and the partial least squares (PLS) regression. The values of C₁₁ and C₄₄ generally increase with the incorporation of Ni or Cr content in light of SE analysis, which is contrary to the inclination of ME analysis in predicting the impact of Ni and Cr elements, and the Ni element demonstrates a pronounced negative competitive ability. The overall competitive relationship among the three alloying elements suggests that increasing the content of Ni and Cr does not contribute to enhancing the elastic constants of alloys, and the phenomenon is also observed in the analysis of elastic moduli. The reason is that the SE analysis fails to account for the aforementioned common influence of multiple alloying elements on the physical properties of alloys. Therefore, the integration of SE analysis and ME analysis is more advantageous in elucidating the hidden competitive mechanism among multiple alloying elements, and offering a more robust theoretical framework for the design of alloy materials. Full article

(This article belongs to the Special Issue Microstructure, Mechanical and Tribological Properties of Alloys)

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12 pages, 928 KiB

Open AccessCommunication

Unlocking New Avenues: Solid-State Synthesis of Molecularly Imprinted Polymers

by Bogdan-Cezar Iacob, Andreea Elena Bodoki, Diogo Filipe Da Costa Carvalho, Antonio Augusto Serpa Paulino, Lucian Barbu-Tudoran and Ede Bodoki

Int. J. Mol. Sci. 2024, 25(10), 5504; https://doi.org/10.3390/ijms25105504 (registering DOI) - 18 May 2024

Abstract

Molecularly imprinted polymers (MIPs) are established artificial molecular recognition platforms with tailored selectivity towards a target molecule, whose synthesis and functionality are highly influenced by the nature of the solvent employed in their synthesis. Steps towards the “greenification” of molecular imprinting technology (MIT) [...] Read more.

Molecularly imprinted polymers (MIPs) are established artificial molecular recognition platforms with tailored selectivity towards a target molecule, whose synthesis and functionality are highly influenced by the nature of the solvent employed in their synthesis. Steps towards the “greenification” of molecular imprinting technology (MIT) has already been initiated by the elaboration of green MIT principles; developing MIPs in a solvent-free environment may not only offer an eco-friendly alternative, but could also significantly influence the affinity and expected selectivity of the resulting binding sites. In the current study the first solvent-free mechanochemical synthesis of MIPs via liquid-assisted grinding (LAG) is reported. The successful synthesis of the imprinted polymer was functionally demonstrated by measuring its template rebinding capacity and the selectivity of the molecular recognition process in comparison with the ones obtained by the conventional, non-covalent molecular imprinting process in liquid media. The results demonstrated similar binding capacities towards the template molecule and superior chemoselectivity compared to the solution-based MIP synthesis method. The adoption of green chemistry principles with all their inherent advantages in the synthesis of MIPs may not only be able to alleviate the potential environmental and health concerns associated with their analytical (e.g., selective adsorbents) and biomedical (e.g., drug carriers or reservoirs) applications, but might also offer a conceptual change in molecular imprinting technology. Full article

(This article belongs to the Special Issue Biomimetic Materials Applied in the Analytical and Biomedical Fields)

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13 pages, 276 KiB

Open AccessArticle

Risk Factors for Therapeutic Failure and One-Year Mortality in Patients with Intramedullary Nail-Associated Infection after Trochanteric and Subtrochanteric Hip Fracture Repair

by Bernadette Pfang, Marco A. Villegas García, Antonio Blanco García, Álvaro Auñón Rubio, Jaime Esteban and Joaquín García Cañete

Antibiotics 2024, 13(5), 463; https://doi.org/10.3390/antibiotics13050463 (registering DOI) - 18 May 2024

Abstract

Despite the implications of trochanteric and subtrochanteric intramedullary (IM) nail infection for patients with hip fracture, little is known about risk factors for therapeutic failure and mortality in this population. We performed a retrospective observational analysis including patients diagnosed with trochanteric and subtrochanteric [...] Read more.

Despite the implications of trochanteric and subtrochanteric intramedullary (IM) nail infection for patients with hip fracture, little is known about risk factors for therapeutic failure and mortality in this population. We performed a retrospective observational analysis including patients diagnosed with trochanteric and subtrochanteric IM nail infection at a Spanish academic hospital during a 10-year period, with a minimum follow-up of 22 months. Of 4044 trochanteric and subtrochanteric IM nail implants, we identified 35 cases of infection during the study period (0.87%), 17 of which were chronic infections. Patients with therapeutic failure (n = 10) presented a higher average Charlson Comorbidity Index (CCI) (5.40 vs. 4.21, p 0.015, CI 0.26–2.13) and higher rates of polymicrobial (OR 5.70, p 0.033, CI 1.14–28.33) and multidrug-resistant (OR 7.00, p 0.027, CI 1.24–39.57) infections. Upon multivariate analysis, polymicrobial infection and the presence of multidrug-resistant pathogens were identified as independent risk factors for therapeutic failure. Implant retention was associated with an increased risk of failure in chronic infection and was found to be an independent risk factor for overall one-year mortality in the multivariate analysis. Our study highlights the importance of broad-spectrum empirical antibiotics as initial treatment of trochanteric and subtrochanteric IM nail-associated infection while awaiting microbiological results. It also provides initial evidence for the importance of implant removal in chronic IM-nail infection. Full article

(This article belongs to the Special Issue Antibiotic Therapy in Implant Related Orthopedic Infections)

15 pages, 4067 KiB

Open AccessArticle

Assessing the Effectiveness of Eslicarbazepine Acetate in Reducing Audiogenic Reflex Seizures in the GASH/Sal Model of Epilepsy

by Jaime Gonçalves-Sánchez, Thomas Ramírez-Santos, Dolores E. López, Jesús M. Gonçalves-Estella and Consuelo Sancho

Biomedicines 2024, 12(5), 1121; https://doi.org/10.3390/biomedicines12051121 (registering DOI) - 18 May 2024

Abstract

Eslicarbazepine acetate (ESL) is a third-generation antiepileptic drug indicated as monotherapy for adults with newly diagnosed epilepsy and as adjunctive therapy for the treatment of partial seizures. Our aim was to assess the effectiveness and safety of both acute and repeated ESL administration [...] Read more.

Eslicarbazepine acetate (ESL) is a third-generation antiepileptic drug indicated as monotherapy for adults with newly diagnosed epilepsy and as adjunctive therapy for the treatment of partial seizures. Our aim was to assess the effectiveness and safety of both acute and repeated ESL administration against reflex audiogenic seizures, as shown by the Genetic Audiogenic Seizures Hamster from Salamanca (GASH/Sal). Animals were subject to the intraperitoneal administration of ESL, applying doses of 100, 150 and 200 mg/kg for the acute study, whereas a daily dose of 100 mg/kg was selected for the subchronic study, which lasted 14 days. In both studies, the anticonvulsant effect of the therapy was evaluated using neuroethological methods. To assess the safety of the treatment, behavioral tests were performed, hematological and biochemical liver profiles were obtained, and body weight was monitored. In addition, the ESL levels in blood were measured after the acute administration of a 200 mg/kg dose. Treatment with ESL caused a reduction in seizure severity. No statistically significant differences were detected between the selected doses or between the acute or repeated administration of the drug. To summarize, the intraperitoneal administration of ESL is safe and shows an anticonvulsant effect in the GASH/Sal. Full article

(This article belongs to the Special Issue Advances in Antiepileptic Drugs)

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12 pages, 3210 KiB

Open AccessArticle

Improving Ammonia Emission Model of Urea Fertilizer Fluidized Bed Granulation System Using Particle Swarm Optimization for Sustainable Fertilizer Manufacturing Practice

by Norhidayah Mohamad, Nor Azlina Ab. Aziz, Anith Khairunnisa Ghazali and Mohd Rizal Salleh

Processes 2024, 12(5), 1025; https://doi.org/10.3390/pr12051025 (registering DOI) - 18 May 2024

Abstract

Granulation is an important class of production processes in food, chemical and pharmaceutical manufacturing industries. In urea fertilizer manufacturing, fluidized beds are often used for the granulation system. However, the granulation processes release ammonia to the environment. Ammonia gas can contribute to eutrophication, [...] Read more.

Granulation is an important class of production processes in food, chemical and pharmaceutical manufacturing industries. In urea fertilizer manufacturing, fluidized beds are often used for the granulation system. However, the granulation processes release ammonia to the environment. Ammonia gas can contribute to eutrophication, which is an oversupply of nitrogen and acidification to the ecosystems. Eutrophication may cause major disruptions of aquatic ecosystems. It is estimated that global ammonia emissions from urea fertilizer processes are approximately at 10 to 12 Tg N/year, which represents 23% of overall ammonia released globally. Therefore, accurate modeling of the ammonia emission by the urea fertilizer fluidized bed granulation system is important. It allows for the system to be operated efficiently and within sustainable condition. This research attempts to optimize the model of the system using the particle swarm optimization (PSO) algorithm. The model takes pressure (Mpa), binder feed rate (rpm) and inlet temperature (°C) as the manipulated variables. The PSO searches for the model’s optimal coefficients. The accuracy of the model is measured using mean square error (MSE) between the model’s simulated value and the actual data of ammonia released which is collected from an experiment. The proposed method reduces the MSE to 0.09727, indicating that the model can accurately simulate the actual system. Full article

(This article belongs to the Special Issue Simulation, Modeling, and Decision-Making Processes in Manufacturing Systems and Industrial Engineering)

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12 pages, 749 KiB

Open AccessArticle

Inhibitory Effects on Staphylococcus aureus Sortase A by Aesculus sp. Extracts and Their Toxicity Evaluation

by Octavian Tudorel Olaru, George Mihai Nitulescu, Andreea Miruna Codreanu, Valentina-Andreea Calmuc, Luanne Venables, Maryna van de Venter, Cerasela Elena Gird, Cosmina-Gabriela Duta-Bratu and Georgiana Nitulescu

Plants 2024, 13(10), 1405; https://doi.org/10.3390/plants13101405 (registering DOI) - 18 May 2024

Abstract

A promising strategy for combating bacterial infections involves the development of agents that disarm the virulence factors of pathogenic bacteria, thereby reducing their pathogenicity without inducing direct lethality. Sortase A, a crucial enzyme responsible for anchoring virulence factors to the cell surface of [...] Read more.

A promising strategy for combating bacterial infections involves the development of agents that disarm the virulence factors of pathogenic bacteria, thereby reducing their pathogenicity without inducing direct lethality. Sortase A, a crucial enzyme responsible for anchoring virulence factors to the cell surface of several pathogenic bacteria, has emerged as a possible target for antivirulence strategies. A series of hippocastanum species (Aesculus pavia, A. parviflora, Aesculus x carnea, and A. hippocastanum) were used to prepare ethanol- and water-based extracts for assessing their effect on Staphylococcus aureus sortase A. The extracts were characterized through HPLC analysis, and their polyphenols content was determined using the Folin–Ciocalteu method. The specific toxicity profile was evaluated in Daphnia magna using the median lethal concentration (LC₅₀) and against the fibroblast MRHF cell line. The half maximal inhibitory concentration (IC₅₀) values on sortase A, determined after 30 min of incubation, ranged from 82.70 to 304.31 µg/mL, with the A. pavia water extract exhibiting the highest inhibitory effect. The assessment of the A. pavia water extract on human fibroblasts revealed no significant signs of toxicity, even at a concentration of 500 µg/mL. This reduced toxicity was further validated through the Daphnia assay. These findings highlight the low toxicity and the potential of this extract as a promising source of future development of bacteria antivirulence solutions. Full article

(This article belongs to the Special Issue Antioxidant in Plants - Discovery, Biosynthesis, Regulation and Application)

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16 pages, 4590 KiB

Open AccessArticle

Among Other Tissues, Short-Term Garlic Oral Treatment Incrementally Improves Indicants of Only Pancreatic Islets of Langerhans Histology and Insulin mRNA Transcription and Synthesis in Diabetic Rats

by Amani M. Al-Adsani and Khaled K. Al-Qattan

Biology 2024, 13(5), 355; https://doi.org/10.3390/biology13050355 (registering DOI) - 18 May 2024

Abstract

Background: The source, mRNA transcription, and synthesis of insulin in the pancreas, in addition to the bile duct and liver, in streptozotocin (STZ)-induced diabetic rats (DR) in response to garlic oral treatment are not yet clear. Objective: This study investigated the accumulative effects [...] Read more.

Background: The source, mRNA transcription, and synthesis of insulin in the pancreas, in addition to the bile duct and liver, in streptozotocin (STZ)-induced diabetic rats (DR) in response to garlic oral treatment are not yet clear. Objective: This study investigated the accumulative effects of continued garlic oral treatment on changes in the pancreas, bile duct, and liver with regards to: 1—Insulin mRNA transcription, synthesis, and concentration in relation to changes in serum insulin (SI); 2—Insulinogenic cells insulin intensity and distribution, proliferation, and morphology. Method: Fasting blood glucose (FBG) and insulin concentration in serum and pancreas (PI) and sources and mRNA transcription in the pancreas, bile duct, and liver in normal rats given normal saline (NR-NS) and DR given either NS (DR-NS) or garlic extract (DR-GE) before and after 1, 4, and 8 weeks of oral treatment were examined. Results: Compared to NR-NS, DR-NS showed a significant increase in FBG and reductions in SI and PI and deterioration in islets histology, associated pancreatic insulin numerical intensities, and mRNA transcription. However, compared to DR-NS, the targeted biochemical, histological, and genetic variables of DR-GE were significantly and incrementally improved as garlic treatment continued. Insulin or its indicators were not detected either in the bile duct or the liver in DR-GE. Conclusions: 8 weeks of garlic oral treatment is enough to incrementally restore only pancreatic islets of Langerhans insulin intensity and insulinogenic cells proliferation, morphology, and distribution. These indices were associated with enhanced pancreatic insulin mRNA transcription and synthesis. Eight weeks of garlic treatment were not enough to stimulate insulinogenesis in either the bile duct or the liver. Full article

(This article belongs to the Special Issue Animal Models of Pancreatic Diseases)

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8 pages, 3304 KiB

Open AccessArticle

Novel Highly Efficient Buried Gratings for Selective Coupling of SPP Waves onto Single Interfaces

by Arif Nabizada, Hamed Tari, Alessandro Bile and Eugenio Fazio

Nanomaterials 2024, 14(10), 878; https://doi.org/10.3390/nano14100878 (registering DOI) - 18 May 2024

Abstract

Diffraction gratings have always been used to effectively couple optical radiation within integrated waveguides. This is also valid for plasmonic structures that support Surface Plasmon Polariton (SPP) waves. Traditional gratings usually excite SPP waves at the interface where they are located or, for [...] Read more.

Diffraction gratings have always been used to effectively couple optical radiation within integrated waveguides. This is also valid for plasmonic structures that support Surface Plasmon Polariton (SPP) waves. Traditional gratings usually excite SPP waves at the interface where they are located or, for thin metal nanostrips, at both interfaces. But reducing the thickness of the metal layer in the presence of a grating has the handicap of increasing the tunnelling of light towards the substrate, which means higher losses and reduced coupling efficiency. In this paper, we design and optimize novel gratings buried within the metallic thin films for selective coupling of SPP waves onto individual interfaces. Compared with traditional superficial gratings, the novel buried ones demonstrate higher efficiency and much lower residual tunnelling of light through the coupling structures. Full article

(This article belongs to the Section Theory and Simulation of Nanostructures)

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13 pages, 1163 KiB

Open AccessArticle

Clinical Characteristics, Patterns of Care, and Treatment Outcomes of Radiation-Associated Sarcomas

by Rohit Raj, Han Gil Kim, Menglin Xu, Tyler Roach, David Liebner, David Konieczkowski and Gabriel Tinoco

Cancers 2024, 16(10), 1918; https://doi.org/10.3390/cancers16101918 (registering DOI) - 18 May 2024

Abstract

Radiation-associated sarcomas (RASs) are rare tumors with limited contemporary data to inform prognostication and management. We sought to identify the clinical presentation, patterns of care, and prognostic factors of RASs. RAS patients treated at a single institution from 2015 to 2021 were retrospectively [...] Read more.

Radiation-associated sarcomas (RASs) are rare tumors with limited contemporary data to inform prognostication and management. We sought to identify the clinical presentation, patterns of care, and prognostic factors of RASs. RAS patients treated at a single institution from 2015 to 2021 were retrospectively reviewed for clinicopathologic variables, treatment strategies, and outcomes. Thirty-eight patients were identified with a median follow-up of 30.5 months. The median age at RAS diagnosis was 68.4 years (27.9–85.4), with a median latency from index radiotherapy (RT) of 9.1 years (3.7–46.3). RAS histologies included angiosarcoma (26%), undifferentiated pleomorphic sarcoma (21%), and osteosarcoma (18%). Most were high-grade (76%). Genomic profiling revealed low tumor mutational burden, frequent inactivating TP53 mutations (44%), CDKN2A deletions (26%), and MYC amplifications (22%), particularly in breast angiosarcomas. Of 38 patients, 33 presented with localized disease, 26 of whom were treated with curative intent. Overall, the median progression-free survival (PFS) was 9.5 months (1.4–34.7), and the overall survival (OS) was 11.1 months (0.6–31.6). Patients with localized vs. metastatic RASs had a longer PFS (HR 3.0 [1.1–8.5]; p = 0.03) and OS (HR, 3.0 [1.04–8.68]; p = 0.03). Among localized RAS patients, high grade was associated with shorter OS (HR, 4.6 [1.04–20.30]; p = 0.03) and resection with longer OS (mean 58.8 vs. 6.1 months, HR, 0.1 [0.03–0.28]; p < 0.001). Among patients undergoing resection, negative margins were associated with improved OS (mean 71.0 vs. 15.5 months, HR, 5.1 [1.4–18.2]; p = 0.006). Patients with localized disease, particularly those undergoing R0 resection, demonstrated significantly better outcomes. Novel strategies are urgently needed to improve treatment outcomes in this challenging group of diseases. Full article

(This article belongs to the Section Clinical Research of Cancer)

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7 pages, 2144 KiB

Open AccessCase Report

Anterior Esthetic Restorations with the Stratified Stamp Technique: A Case Report

by Camillo D’Arcangelo, Matteo Buonvivere and Francesco De Angelis

Biomimetics 2024, 9(5), 299; https://doi.org/10.3390/biomimetics9050299 (registering DOI) - 18 May 2024

Abstract

Anterior teeth restoration represents a challenge for dentists, who often rely on the dental technician’s wax-up. The proposed Stratified Stamp Technique (SST) allows for clinically reproducing the wax-up in a quick and easy way. A patient with fractures and discoloration on the upper [...] Read more.

Anterior teeth restoration represents a challenge for dentists, who often rely on the dental technician’s wax-up. The proposed Stratified Stamp Technique (SST) allows for clinically reproducing the wax-up in a quick and easy way. A patient with fractures and discoloration on the upper central incisors was treated with resin-based composite direct restorations. Using SST, a 1 mm thick thermoformed polyethylene-terephthalate-glycol (PETG) template, based on the technician’s wax-up, was produced. Enamel Selective Area Reduction (SAR) was performed to guarantee adequate space for the restorations, and the fracture margins were rounded and finished. Traditional layering procedures according to the five color dimensions of teeth were performed, except for the final labial layer, which was realized with warm composite loaded inside the template and polymerized through it, in order to ensure accurate tooth morphology reproduction. SST offers a reliable method for transferring technician’s wax-up morphology to direct composite restorations in anterior teeth. Compared with other methods, SST allows for better isolation with a rubber dam and permits traditional layering with multiple composite shades, thus leading to satisfactory esthetic outcomes. Full article

(This article belongs to the Special Issue Dentistry and Craniofacial District: The Role of Biomimetics—Second Edition)

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16 pages, 971 KiB

Open AccessReview

Therapeutic Effects of Anti-Inflammatory and Anti-Oxidant Nutritional Supplementation in Retinal Ischemic Diseases

by Deokho Lee, Zhongjie Fu, Ann Hellstrom and Lois E. H. Smith

Int. J. Mol. Sci. 2024, 25(10), 5503; https://doi.org/10.3390/ijms25105503 (registering DOI) - 18 May 2024

Abstract

Appropriate nutrients are essential for cellular function. Dietary components can alter the risk of systemic metabolic diseases, including cardiovascular diseases, cancer, diabetes, and obesity, and can also affect retinal diseases, including age-related macular degeneration, diabetic retinopathy, and glaucoma. Dietary nutrients have been assessed [...] Read more.

Appropriate nutrients are essential for cellular function. Dietary components can alter the risk of systemic metabolic diseases, including cardiovascular diseases, cancer, diabetes, and obesity, and can also affect retinal diseases, including age-related macular degeneration, diabetic retinopathy, and glaucoma. Dietary nutrients have been assessed for the prevention or treatment of retinal ischemic diseases and the diseases of aging. In this article, we review clinical and experimental evidence concerning the potential of some nutritional supplements to prevent or treat retinal ischemic diseases and provide further insights into the therapeutic effects of nutritional supplementation on retinopathies. We will review the roles of nutrients in preventing or protecting against retinal ischemic diseases. Full article

(This article belongs to the Special Issue Emerging Roles of Inflammation in Retinal and Optic Nerve Pathologies)

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