Pithiness is one of the physiological diseases of radishes, which is accompanied by the accumulation of reactive oxygen species (ROS) during the sponging of parenchyma tissue in the fleshy roots. A respiratory burst oxidase homolog (Rboh, also known as NADPH oxidase) is a key enzyme that catalyzes the production of ROS in plants. To understand the role of Rboh genes in radish pithiness, herein, 10 RsRboh gene families were identified in the genome of Raphanus sativus using Blastp and Hmmer searching methods and were subjected to basic functional analyses such as phylogenetic tree construction, chromosomal localization, conserved structural domain analysis, and promoter element prediction. The expression profiles of RsRbohs in five stages (Pithiness grade = 0, 1, 2, 3, 4, respectively) of radish pithiness were analyzed. The results showed that 10 RsRbohs expressed different levels during the development of radish pithiness. Except for RsRbohB and RsRbohE, the expression of other members increased and reached the peak at the P2 (Pithiness grade = 2) stage, among which RsRbohD1 showed the highest transcripts. Then, the expression of 40 genes related to RsRbohD1 and pithiness were analyzed. These results can provide a theoretical basis for improving pithiness tolerance in radishes. Full article

Ejector-cleaning devices for abrasive jet machining have various practical applications. The working nozzle is one of the device’s key elements affecting the treated surface quality. There arises the necessity for new approaches to achieving an efficiency increase in abrasive jet equipment nozzles, namely their design improvement and further development of a new, relatively cheap but effective technology for their manufacturing and maintenance. This technology should allow for the high durability of nozzles without being essential for the hardness or wear resistance parameters of the material used for manufacturing. The nozzle should be designed as a long-length perforated insert to allow for radial airflow, forcing the abrasive material (river sand) from the inner walls of the nozzle’s working surface to reduce its friction with the abrasive material. This will result in new wear-out conditions, providing an essential decrease in the wear-out of a nozzle’s working surface. The article aims to develop a more effective design for the working nozzle based on the perforated insert application. The task was set to provide a more detailed experimental and theoretical study of the processes in perforated nozzles to improve their effectiveness. The research resulted in a new design for nozzles with higher efficiency. Full article

Egypt has the potential to generate a significant amount of energy from renewable technologies, in particular solar PV, concentrated solar power (CSP), and onshore and offshore wind. The energy sector is reliant on fossil fuels, particularly natural gas, for electricity production and is at risk of locking itself into a high carbon pathway. Globally, reducing greenhouse gas (GHG) emissions associated with national energy sectors is a target outlined in the UN’s Paris Agreement. To reduce carbon dioxide (CO₂) emissions associated with a higher dependence on fossil fuels, Egypt must consider upscaling renewable energy technologies (RETs) to achieve a clean energy transition (CET). This research modelled six scenarios using clicSAND for OSeMOSYS to identify the technologies and policy target improvements that are needed to upscale RETs within Egypt’s energy sector. The results showed that solar PV and onshore wind are key technologies to be upscaled to contribute towards Egypt’s CET. The optimal renewable target is the International Renewable Energy Agency’s (IRENA) target of 53% of electricity being sourced from RETs by 2030, which will cost USD 16.4 billion more up to 2035 than Egypt’s current Integrated Sustainable Energy Strategy (ISES) target of 42% by 2035; it also saves 732.0 MtCO₂ over the entire modelling period to 2070. Socio-economic barriers to this transition are considered, such as recent discoveries of natural gas reserves combined with a history of energy insecurity, political instability impacting investor confidence, and a lack of international climate funding. The paper concludes with policy recommendations that would enable Egypt to progress towards achieving a CET. Full article

The filling efficiency during the hot embossing process at micro scale is essential for micro-component replication. The presence of the unfilled area is often due to the inadequate behavior law applied to the embossed materials. This research consists of the identification of viscoplastic law (two-layer viscoplastic model) of polymers and the optimization of processing parameters. Mechanical tests have been performed for two polymers at 20 °C and 30 °C above their glass transition temperature. The viscoplastic parameters are characterized based on stress–strain curves from the compression tests. The influences of imposed displacement, temperature, and friction on mold filling are investigated. The processing parameters are optimized to achieving the complete filling of micro cavities. The replication of a micro-structured cavity has been effectuated using this process and the experimental observations validate the results in the simulation, which confirms the efficiency of the proposed numerical approach. Full article

The developments in manufacturing technologies are expected to reduce energy input without compromising product quality. Regarding the material densification process, numerical simulation methods are applied to achieve this goal. In this case, relevant material models are built using functions that describe the variation in mechanical parameters of the material in question due to its deformation. The literature review conducted for this research has revealed a shortage of experimental research methods allowing a determination of the coefficient of friction at low temperatures, approximately 200 K. This article proposes a method for determining the friction coefficient of dry ice sliding against steel. The experimental results were analysed to obtain several functions describing the variation in the coefficient of friction. These functions were then compared using goodness-of-fit indexes. Finally, two functions with similar goodness-of-fit values were chosen. The findings of this research project will complement the already available information and may be used in various research and implementation projects related to the development or improvement of currently used crystallised carbon dioxide conversion processes. Full article

Access control systems are part of the overall protection of objects. It is often the first system with which it is necessary to start the contact system; therefore, it is necessary to ensure its proper functioning. In the event of a malfunction, it can cause downtime in production, and it is triggered by a bad replacement of workers. Access control systems have their own specificities that need to be considered when designing security. Poor selection of access control devices can cause inefficient system functionality, resulting in downtime and loss. Based on experimental testing and related work in access control systems, this paper discusses the possibilities of testing the throughput of access control systems. The manuscript presents the design of a unique test device that can be used in the assessment of the reliability and throughput of access control systems. With the help of tests, we were able to determine the probability of downtime due to inappropriately set time intervals for changing employees on a work shift. Full article

This study was conducted to compare and analyze whether Pilates exercise and yoga exercise help improve the performance of female fencers and prevent injury, and the dynamic balance test (LQ-YBT) and functional movement screening (FMS) test score of the elite adult female fencers were compared and analyzed as evaluation indicators. Participants were randomly classified into Pilates (n = 10) and yoga groups (n = 10), members of which took part in 50 min of exercise (5 min of warm-up, 40 min of main exercise, and 5 min of cool-down) twice weekly for eight weeks. The results obtained from this study were analyzed via independent t-test and 2-way ANOVA. The results were as follows: LQ-YBT measures (reaching distance) increased significantly for both groups, as did FMS scores (deep squat, hurdle step, inline lunge, shoulder mobility, active straight-leg raise, trunk-stability push-up, and rotary stability). These results suggest that Pilates exercise and yoga exercise might be likely effective in improving the performance of adult female fencers and injury prevention by increasing their dynamic balance ability and functional movement. Full article

This study examines the effect of the ethical climate on job satisfaction in the nursing profession and how this effect occurs through trust in colleagues. A survey design was used to collect data from 407 nurses working in hospitals in Mersin City, Turkey. The study used self-report questionnaires to measure the ethical climate, job satisfaction, and trust. The data were analyzed using SPSS 22 software, which incorporated confirmatory factor analysis to assess construct validity and the bootstrap method to examine mediation effects. Ethical protocols were rigorously observed. The study clearly demonstrated a significant positive relationship between the ethical climate and job satisfaction. The trust variable fully mediates the relationship between the ethical climate and job satisfaction. The findings make a strong case for the importance of an ethical climate in nursing, synthesizing findings from the previous literature. The findings of this study demonstrate the necessity of assessing different attitudes and behaviors in order to promote expected behaviors in the unique and intense working environment of nurses. These behaviors may diverge from theoretical predictions. The results of this study also revealed that an ethical climate and positive work attitudes among nurses have a significant effect on job satisfaction, which is an important input for productivity. Full article

This systematic review evaluates the clinical outcomes and molecular predictors of response to pembrolizumab in patients with advanced and metastatic cervical cancer. We adhered to the PRISMA guidelines for systematic reviews, conducting a database search in PubMed, Scopus, and Embase. The eligibility criteria centered on clinical outcomes, including the overall survival (OS), progression-free survival (PFS), and immune-related biomarkers post-pembrolizumab therapy. We included both prospective and retrospective studies that detailed clinical outcomes and molecular characteristics predictive of therapeutic response. Our search yielded six studies involving 846 patients treated with pembrolizumab from 2017 to 2022. The meta-analysis of these studies showed that pembrolizumab, used as monotherapy or in combination with chemotherapy, extended the OS by a weighted median of 10.35 months and the PFS by 8.50 months. The treatment demonstrated a pooled objective response rate (ORR) of 22.39%, although the I² test result of 67.49% showed a high heterogeneity among the studies. Notably, patients with high PD-L1 expression (CPS ≥ 10) experienced improved outcomes in terms of the PFS and OS. The most common complications were fatigue, diarrhea, and immune-related adverse events. Pembrolizumab significantly enhances clinical outcomes in metastatic cervical cancer, particularly among patients with high PD-L1 expression. The drug maintains a good safety profile, reinforcing its treatment potential for patients with advanced and metastatic cervical cancer. Future studies should explore long-term effects and strategies to integrate pembrolizumab optimally into current treatment regimens, aiming to maximize patient benefits and effectively manage side effects. Full article

This study aimed to investigate the effects of the Mediterranean diet (MD), combined with curcumin and resveratrol supplementation, on disease activity, serum inflammatory markers, and quality of life in patients with mild-to-moderate active ulcerative colitis (UC). This study was designed as a prospective multicenter three-arm randomized controlled trial. Participants were randomized to the MD, MD + curcumin, and MD + resveratrol groups. All participants were placed on the MD for 8 weeks. The MD + curcumin group also received 1600 mg/day of curcumin supplementation, whereas the MD + resveratrol group received 500 mg/day of resveratrol supplementation for 8 weeks. Anthropometric measurements, Truelove–Witts Index, Short Form-36, Inflammatory Bowel Disease Questionnaire, Mediterranean Diet Adherence Scale (MEDAS), and laboratory tests were performed at baseline and postintervention. Within-group comparisons showed that MD, MD + curcumin, and MD + resveratrol interventions were effective in reducing disease activity and inflammation and improving quality of life in individuals with UC (p < 0.05). Between-group comparisons revealed no significant difference in all parameters except for the pain subparameter of SF-36 and the MEDAS score (p < 0.05). The MD is an effective and safe intervention to be used in clinical practice in individuals with UC. Full article

Powder deposition is a very important aspect of PBF-based additive manufacturing processes. Discrete Element Method (DEM) is commonly utilized by researchers to examine the physically complex aspects of powder-spreading methods. This work focuses on vibration-assisted doctor blade powder recoating. The aim of this work is to use experiment-verified DEM simulations in combination with Taguchi Design of Experiments (DoE) to identify optimum spreading parameters based on robust layer quality criteria. The verification of the used powder model is performed via angle of repose and angle of avalanche simulation–experiment cross-checking. Then, four criteria, namely layer thickness deviation, surface coverage ratio, surface root-mean-square roughness and true packing density, are defined. It has been proven that the doctor blade’s translational speed plays the most important role in defining the quality of the deposited layer. The true packing density was found to be unaffected by the spreading parameters. The vertical vibration of the doctor blade recoater was found to have a beneficial effect on the quality of the deposited layer. Ultimately, a weighted mean quality criteria analysis is mapped out. Skewness and kurtosis were proven to function as effective indicators of layer quality, showing a linear relation to the weighted means of the defined quality criteria. The specific weights that optimize this linearity were identified. Full article

A novel technique suitable for the propulsion of small unmanned aerial vehicles (UAV) is discussed in this paper. This approach utilizes the rotational energy of airborne gyro rotors and converts it into translational propulsion for the vehicle. The energy conversion is achieved by generating precisely directed centrifugal force pulses through short-duration rotor unbalances. The accurate control of the timing and magnitude of these unbalances is crucial for successful propulsion generation. Our first-order theory of controlled unbalance propulsion (CUP) predicts the potential for achieving high translational accelerations and vehicle velocities up to orbital levels. Power-saving levitation of UAVs can be attained. In this paper, we provide traceable evidence that pulsed centrifugal propulsion is based on well-established laws of physics and can be realized using state-of-the-art technologies. Full article

Studies show that feedback greatly improves student learning outcomes, but achieving this level of personalization at scale is a complex task, especially in the diverse and open environment of Massive Open Online Courses (MOOCs). This research provides a novel method for using cutting-edge artificial intelligence technology to enhance the feedback mechanism in MOOCs. The main goal of this research is to leverage AI’s capabilities to automate and refine the MOOC feedback process, with special emphasis on courses that allow students to learn at their own pace. The combination of LangChain—a cutting-edge framework specifically designed for applications that use language models—with the OpenAI API forms the basis of this work. This integration creates dynamic, scalable, and intelligent environments that can provide students with individualized, insightful feedback. A well-organized assessment rubric directs the feedback system, ensuring that the responses are both tailored to each learner’s unique path and aligned with academic standards and objectives. This initiative uses Generative AI to enhance MOOCs, making them more engaging, responsive, and successful for a diverse, international student body. Beyond mere automation, this technology has the potential to transform fundamentally how learning is supported in digital environments and how feedback is delivered. The initial results demonstrate increased learner satisfaction and progress, thereby validating the effectiveness of personalized feedback powered by AI. Full article

Ganoderma lucidum spore powder, valued for its nutritional and medicinal properties, contains polysaccharides crucial for its efficacy. However, the complex structural nature of these polysaccharides necessitates further investigation to fully realize their potential. This study aimed to investigate the effects of acid heat treatment on Ganoderma lucidum spore polysaccharides (GLSPs) to enhance their properties and application in antitumor activity. The GLSP was obtained via acid heat treatment, concentration, and centrifugal separation. This process led to a notable reduction in polysaccharide molecular weight, increasing water solubility and bioavailability. Analytical techniques including NMR spectroscopy and methylation analysis revealed a polysaccharide composition comprising four distinct monosaccharides, with molecular weights of 3291 Da (Mw) and 3216 Da (Mn). Six different linkage modes were identified, with a molar ratio of 1:5:2:3:4:3. In vivo experiments demonstrated the GLSP’s significant inhibitory effect on the growth of four tumor models (sarcoma S180, Lewis lung cancer, liver cancer H22, and colon cancer C26) in mice, with no observed toxicity. These findings suggest the GLSP’s potential as an antitumor therapeutic agent for clinical use. Full article

Glycyrrhetinic acid (GA) is a saponin compound, isolated from licorice (Glycyrrhiza glabra), which has been wildly explored for its intriguing pharmacological and medicinal effects. GA is a triterpenoid glycoside displaying an array of pharmacological and biological activities, including anti-inflammatory, anti-bacterial, antiviral and antioxidative properties. In this study, we investigated the underlying mechanisms of GA on acne vulgaris through network pharmacology and proteomics. After the intersection of the 154 drug targets and 581 disease targets, 37 therapeutic targets for GA against acne were obtained. A protein–protein interaction (PPI) network analysis highlighted TNF, IL1B, IL6, ESR1, PPARG, NFKB1, STAT3 and TLR4 as key targets of GA against acne, which is further verified by molecular docking. The experimental results showed that GA inhibited lipid synthesis in vitro and in vivo, improved the histopathological damage of skin, prevented mast cell infiltration and decreased the level of pro-inflammatory cytokines, including TNF-α, IL-1β and IL-6. This study indicates that GA may regulate multiple pathways to improve acne symptoms, and the beneficial effects of GA against acne vulgaris might be through the regulation of sebogenesis and inflammatory responses. Full article

In the realm of commercial trade, the appearance quality of wheat is a crucial metric for assessing its value and grading. Traditionally, evaluating wheat appearance quality is a manual process conducted by inspectors, which is time-consuming, laborious, and error-prone. In this research, we developed an intelligent detection system for wheat appearance quality, leveraging state-of-the-art neural network technology for the efficient and standardized assessment of wheat appearance quality. Our system was meticulously crafted, integrating high-performance hardware components and sophisticated software solutions. Central to its functionality is a detection model built upon multi-grained convolutional neural networks. This innovative setup allows for the swift and precise evaluation and categorization of wheat quality. Remarkably, our system achieved an exceptional overall recognition accuracy rate of 99.45% for wheat grain categories, boasting a recognition efficiency that was approximately five times faster than manual recognition processes. This groundbreaking system serves as a valuable tool for assisting inspectors, offering technical support for customs quarantine, grain reserves, and food safety. Full article

Harvested wampee fruit is susceptible to disease, resulting in postharvest losses. Acidic electrolyzed water (AEW), a safe and innovative sterilization technology, plays a role in enhancing disease resistance in harvested produce. In this study, the efficacy of AEW in delaying wampee disease development was assessed, along with its association with disease resistance metabolism. Wampee fruit was treated with AEW (pH 2.5) at different available chlorine concentrations (ACCs) (20, 40, 60, and 80 mg/L) and subsequently stored at 25 °C for 8 days. Results revealed that 40 mg/L ACC in AEW (pH 2.5) was most effective in improving the postharvest quality of wampee fruit. Compared with control wampee fruit, those treated with 40 mg/L ACC in AEW exhibited lower incidence of fruit disease, higher pericarp lignin content, and higher activities of pericarp disease resistance enzymes (DREs), such as cinnamate-4-hydroxylase, phenylalanine ammonia-lyase, chitinase, β-1,3-glucanase, polyphenol oxidase, 4-coumarate CoA ligase, and cinnamyl alcohol dehydrogenase. These results suggested that AEW elevated DRE activities, promoted lignin accumulation, and ultimately enhanced disease resistance, suppressed disease development, and improved storage quality in harvested wampee fruit. Consequently, AEW emerged as a safe technology to mitigate the disease development and enhance the storage quality of harvested wampee fruit. Full article

Sustainable plant production requires less use of synthetic chemicals in plant nutrition and protection. Microbial products are among the most promising substitutes for chemicals. With the increasing popularity and availability of such products, it has become obligatory to use different microbes together. The effect of this has been tested in several studies, but their results have sometimes been contradictory depending on the microbial strains tested and the mode of application. We tested the effect of two commercially available antagonists and Funneliformis mosseae alone and in combination on tomato. Mycorrhizal treatment increased plant growth and yield, both alone and combined with the antagonists; however, mycorrhizal root colonization was not influenced by the antagonist. This treatment also led to a slight decrease in the occurrence of Trichoderma spp. on tomato roots but did not impede the colonization of roots by the applied Trichoderma strain. Our result confirmed that Trichoderma asperellum (T34) and Streptomyces griseoviridis (K61) can be safely combined with arbuscular mycorrhizal fungi (AMF), namely with F. mosseae. Full article

Osteopontin (OPN) is a glycoprotein involved in Th1 and Th17 differentiation, and inflammation and tissue remodeling. OPN is a biomarker of disease activity in patients with autoimmune inflammatory conditions. This study aimed to assess the diagnostic and prognostic value of OPN in interstitial lung diseases (ILDs). Between May 2016 and October 2019, 344 patients with ILD were recruited at the Hospital Universitario Marqués de Valdecilla (Spain) and were prospectively followed-up. This study involved the determination of OPN serum levels by ELISA and OPN RNA expression quantified using qPCR. Six genetic polymorphisms in OPN (rs28357094, rs2853749, rs2853750, rs11728697, rs7695531, and rs1126616) were genotyped using TaqMan assays. OPN serum levels were also assessed in 140 healthy controls. OPN serum levels (median [interquartile range]) were significantly higher in ILD patients than in controls (1.05 [0.75–1.51] ng/mL versus 0.81 [0.65–0.98] ng/mL in healthy controls; p < 0.01). OPN serum levels were inversely correlated with the forced vital capacity. OPN serum levels were also higher in ILD patients who died or underwent lung transplantation when compared with the remaining ILD patients (1.15 [0.80–1.72] ng/mL versus 0.99 [0.66–1.32] ng/mL; p = 0.05). Survival worsened in ILD patients with OPN > 1.03 ng/mL at 1, 3, and 5 years. No statistically significant differences in the genetic frequencies of OPN polymorphisms or the RNA expression were found among the different ILD groups. Elevated levels of OPN in the serum may be a useful indicator in identifying patients with ILD who are more likely to experience poor outcomes. Full article

The orientation-dependent mechanical behaviors of metallic alloys are governed by deformation mechanisms, but the underlying physics remain to be explored. In this work, the mechanical responses along different orientations and behind the mechanisms of BCC-Fe are investigated by performing molecular dynamic simulations. It is found that the mechanical properties of BCC-Fe exhibit apparent anisotropic characteristics. The <100>-oriented BCC-Fe presents a Young’s modulus of E = 147.56 GPa, a strength of σ_y = 10.15 GPa, and a plastic strain of ε_y = 0.084 at the yield point, whereas the <111> orientation presents E = 244.84 GPa, σ_y = 27.57 GPa, and ε_y = 0.21. Based on classical dislocation theory, the reasons for such orientation-dependent mechanical behaviors are analyzed from the perspective of thermo-kinetic synergy upon deformation. It turns out that the anisotropic mechanical responses of BCC-Fe are associated with the magnitude of the thermodynamic driving force (ΔG) and kinetic energy barrier (Q) for dislocation motion, which dominate the corresponding deformation mechanism. Compared with the low ΔG (6.395 GPa) and high Q (11.95 KJ/mol) of the <100>-oriented BCC-Fe dominated by deformation twinning, the <111> orientation governed by dislocation slip presents a high ΔG (17.37 GPa) and low Q (6.45 KJ/mol). Accordingly, the orientation-dependent deformation behaviors of BCC-Fe are derived from the thermo-kinetic synergy for dislocation motion. Full article

Two-dimensional (2D) van der Waals layered materials have been explored in depth. They can be vertically stacked into a 2D heterostructure and represent a fundamental way to explore new physical properties and fabricate high-performance nanodevices. However, the controllable and scaled growth of non-layered quasi-2D materials and their heterostructures is still a great challenge. Here, we report a selective two-step growth method for high-quality single crystalline CrTe/WSe₂ and CrTe/MoS₂ heterostructures by adopting a universal CVD strategy with the assistance of molten salt and mass control. Quasi-2D metallic CrTe was grown on pre-deposited 2D transition metal dichalcogenides (TMDC) under relatively low temperatures. A 2D CrTe/TMDC heterostructure was established to explore the interface’s structure using scanning transmission electron microscopy (STEM), and also demonstrate ferromagnetism in a metal–semiconductor CrTe/TMDC heterostructure. Full article