The complex mechanical system of a rice combine harvester not only has various excitation sources, but also, the vibration transmission path between each working device and the vibration contribution characteristics to the frame are not clear, so it is difficult to perform a reduction vibration design for the sharp vibration of the rice combine harvester frame. Therefore, based on the comparison and improvement of multiple classical transfer path analysis methods, this paper analyzed the vibration transfer characteristics and transfer characteristics of each harvester by the discrete time matrix method and operating path method. In the Experimental section, through the vibration characteristic experiment firstly, this paper obtained the power spectrum variation and the most needed optimized path in the transmission path of each device under each operating condition. Secondly, through frame simulation analysis under the exciting force, we obtained the vibration damping areas that needs to be optimized. Finally, the damping optimization experiment connected with the vibration characteristic experiment, and the excitation force simulation analysis was performed. The results of the damping optimization experiment displayed that the maximum change value of the vibration acceleration of the cutting table decreased from 7.862 m·s⁻² to 3.522 m·s⁻², decreasing by 55.2%, and the peak amplitude of the multipoint test in the cab was 5.4, 5.3, 1.7 and 2.0 μm, respectively, which was significantly reduced, so the optimization effect was significant. This study provides theoretical support for the vibration reduction optimization of a rice combine harvester frame. Full article

It is important to reduce the building load and downsize the heat source equipment capacity during construction or renovation carried out toward the achievement of carbon neutrality by 2050 in Japan. However, this sometimes results in the oversizing of the heat source equipment capacity, despite the fact that designers are engaged in the implementation of safety designs while attempting to balance between cost and risk. This study investigated the simultaneous usage ratio of heating based on occupant behavior in an apartment building with the aim of optimizing this capacity. This ratio was defined as a peak load-based approach rather than simultaneity based on the number of people using the system. First, the analysis was conducted for the heating load characteristics for each dwelling unit and each household composition. The subject of this case study was an apartment building located in Sapporo, Japan. Based on these data, a method for creating the curve of the simultaneous usage ratio to avoid a combinatorial explosion was suggested. As a result, the ratio created for about 200 dwelling units was 53.6% in an apartment building and generally stabilized when the number of dwelling units exceeded 30. Finally, a case study was attempted to analyze the influence of changes in household composition on the ratio. If the method proposed in this study for creating the curve of simultaneous usage ratios were to be applied in not only this case study but also in case studies of non-residential buildings such as offices, new results about the curves of ratios that differ from those of apartment buildings could be obtained. Therefore, this case study provides a methodology for statistically quantifying the simultaneous usage ratio as one of the factors in determining the appropriate heat source equipment capacity in the design stage. Full article

Glioblastoma (GBM) is the most common malignant brain tumor in adults. Despite advancements in treatment, the prognosis for patients with GBM remains poor due to its aggressive nature and resistance to therapy. CRISPR-based genetic screening has emerged as a powerful tool for identifying genes crucial for tumor progression and treatment resistance, offering promising targets for tumor therapy. In this review, we provide an overview of the recent advancements in CRISPR-based genetic screening approaches and their applications in GBM. We highlight how these approaches have been used to uncover the genetic determinants of GBM progression and responsiveness to various therapies. Furthermore, we discuss the ongoing challenges and future directions of CRISPR-based screening methods in advancing GBM research. Full article

Given the crucial role of the personalized management and treatment of hearing loss (HL), etiological investigations are performed early on, and genetic analysis significantly contributes to the determination of most syndromic and nonsyndromic HL cases. Knowing hundreds of syndromic associations with HL, little comprehensive data about HL in genomic disorders due to microdeletion or microduplications of contiguous genes is available. Together with the description of a new patient with a novel 3.7 Mb deletion of the Xq21 critical locus, we propose an unreported literature review about clinical findings in patients and their family members with Xq21 deletion syndrome. We finally propose a comprehensive review of HL in contiguous gene syndromes in order to confirm the role of cytogenomic microarray analysis to investigate the etiology of unexplained HL. Full article

Due to the frequent impact of external risks, scientific tourism risk assessment has become the primary task to be implemented in the process of tourism development. Especially with the development of self-driving travel, cross-regional tourism corridors have become an important tourism carrier. However, compared to traditional fixed-location tourism, cross-regional tourism introduces a more intricate landscape of risks. Therefore, there is a pressing need to assess the tourism risks inherent in these corridors. There are many cross-regional tourism corridors in the Tibetan Plateau, but the natural environment of the Tibetan Plateau brings great risks to these tourism corridors. That is why this study focuses on the Tibetan Plateau’s tourism corridors, employing methodologies such as the Analytic Hierarchy Process, entropy weight method, geographic information systems (GIS) spatial analysis, and others to delve into their tourism risk profiles and the influencing factors. Our findings reveal elevated tourism risks across the Tibetan Plateau’s corridors, notably concentrated along the Yunnan–Tibet Line, north Sichuan–Tibet Line, Xinjiang–Tibet Line, Tangfan Ancient Road, Qinghai–Tibet Line, and south Sichuan–Tibet Line. Furthermore, Geodetector was employed to scrutinize the factors influencing tourism risk within the Tibetan Plateau’s corridors, identifying tourism resource endowment, geographical location, precipitation patterns, and economic foundations as primary influencers. Notably, the interaction between these factors exacerbates the overall tourism risk. These insights significantly contribute to the field of tourism risk research and provide a scientific basis for formulating robust tourism safety management strategies within the Tibetan Plateau region. Full article

Perilla frutescens var. acuta (Lamiaceae) is widely used not only as an oil or a spice, but also as a traditional medicine to treat colds, coughs, fever, and indigestion. As an ongoing effort, luteolin-7-O-diglucuronide (1), apigenin-7-O-diglucuronide (2), and rosmarinic acid (3) isolated from P. frutescens var. acuta were investigated for their anti-adipogenic and thermogenic activities in 3T3-L1 cells. Compound 1 exhibited a strong inhibition against adipocyte differentiation by suppressing the expression of Pparg and Cebpa over 52.0% and 45.0%, respectively. Moreover, 2 inhibited the expression of those genes in a dose-dependent manner [Pparg: 41.7% (5 µM), 62.0% (10 µM), and 81.6% (50 µM); Cebpa: 13.8% (5 µM), 18.4% (10 µM), and 37.2% (50 µM)]. On the other hand, the P. frutescens var. acuta water extract showed moderate thermogenic activities. Compounds 1 and 3 also induced thermogenesis in a dose-dependent manner by stimulating the mRNA expressions of Ucp1, Pgc1a, and Prdm16. Moreover, an LC-MS/MS chromatogram of the extract was acquired using UHPLC-MS² and it was analyzed by feature-based molecular networking (FBMN) and the Progenesis QI software (version 3.0). The chemical profiling of the extract demonstrated that flavonoids and their glycoside derivatives, including those isolated earlier as well as rosmarinic acid, are present in P. frutescens var. acuta. Full article

The gut microbiota constitutes a complex ecosystem that has an important impact on host health. In this study, genetically engineered zebrafish with inducible nitric oxide synthase (iNOS or NOS2) knockout were used as a model to investigate the effects of nos2a/nos2b gene single knockout and nos2 gene double knockout on intestinal microbiome composition and function. Extensive 16S rRNA sequencing revealed substantial changes in microbial diversity and specific taxonomic abundances, yet it did not affect the functional structure of the intestinal tissues. Notably, iNOS-deficient zebrafish demonstrated a decrease in Vibrio species and an increase in Aeromonas species, with more pronounced effects observed in double knockouts. Further transcriptomic analysis of the gut in double iNOS knockout zebrafish indicated significant alterations in immune-related and metabolic pathways, including the complement and PPAR signaling pathways. These findings underscore the crucial interplay between host genetics and gut microbiota, indicating that iNOS plays a key role in modulating the gut microbial ecology, host immune system, and metabolic responses. Full article

Recently, as global climate change and local disturbances such as wildfires continue, long- and short-term changes in the high-latitude vegetation systems have been observed in various studies. Although remote sensing technology using optical satellites has been widely used in understanding vegetation dynamics in high-latitude areas, there has been limited understanding of various landscape changes at different spatiotemporal scales, their mutual relationships, and overall long-term landscape changes. The objective of this study is to devise a change monitoring strategy that can effectively observe landscape changes at different spatiotemporal scales in the boreal ecosystems from temporally sparse time series remote sensing data. We presented a new post-classification-based change analysis scheme and applied it to time series Landsat data for the central Yakutian study area. Spectral variability between time series data has been a major problem in the analysis of changes that make it difficult to distinguish long- and short-term land cover changes from seasonal growth activities. To address this issue effectively, two ideas in the time series classification, such as the stepwise classification and the lateral stacking strategies were implemented in the classification process. The proposed classification results showed consistently higher overall accuracies of more than 90% obtained in all classes throughout the study period. The temporal classification results revealed the distinct spatial and temporal patterns of the land cover changes in central Yakutia. The spatiotemporal distribution of the short-term class illustrated that the ecosystem disturbance caused by fire could be affected by local thermal and hydrological conditions of the active layer as well as climatic conditions. On the other hand, the long-term class changes revealed land cover trajectories that could not be explained by monotonic increase or decrease. To characterize the long-term land cover change patterns, we applied a piecewise linear model with two line segments to areal class changes. During the former half of the study period, which corresponds to the 2000s, the areal expansion of lakes on the eastern Lena River terrace was the dominant feature of the land cover change. On the other hand, the land cover changes in the latter half of the study period, which corresponds to the 2010s, exhibited that lake area decreased, particularly in the thermokarst lowlands close to the Lena and Aldan rivers. In this area, significant forest decline can also be identified during the 2010s. Full article

Street food may be a vehicle of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) to humans. Foods contaminated with ARB entail serious problems or challenges in the fields of medical care, animal husbandry, food industry, and public health worldwide. The objectives of this systematic review were to identify and evaluate scientific reports associated with ARB isolated from various street foods. “Preferred reporting items for systematic reviews and meta-analysis” (PRISMA) guidelines were followed. The bibliographic material covers a period from January 2015 to April 2024. Six electronic scientific databases were searched individually for full-text articles; only those papers that met the inclusion and exclusion criteria were selected. Seventeen papers were included in this systematic review. This study highlighted the wide distribution of ARB resistant to β-lactams and other antibiotics, posing significant health risks to consumers. High resistance levels were observed for antibiotics such as ampicillin, ceftriaxone, and tetracycline, while some antibiotics, such as ceftazidime, clavulanic acid, cefoperazone, cotrimoxazole, doxycycline, doripenem, fosfomycin, vancomycin, and piperacillin-tazobactam, demonstrated 100% susceptibility. The prevalence of ARB in street foods varied between 5.2% and 70.8% among different countries. The multiple resistance of various bacteria, including Escherichia coli, Staphylococcus, Salmonella, and Klebsiella, to multiple classes of antibiotics, as well as environmental factors contributing to the spread of antibiotic resistance (AR), emphasize the urgent need for comprehensive approaches and coordinated efforts to confront antimicrobial resistance (AMR) under the “One Health” paradigm. Full article

This study addresses the challenges of the expanding global online retail market by developing an innovative inventory management strategy that promotes sustainable development. We propose an integrated planning model that utilizes a newly enhanced Parallel Chicken Swarm Optimization (PCSO) algorithm alongside the established Economic Order Quantity (EOQ) model and the (s, S) inventory policy. Our model demonstrates its effectiveness through simulation experiments, showcasing its ability to dynamically adjust inventory thresholds based on daily forecasted demand, which significantly optimizes replenishment strategies. Notably, our model achieves a total cost reduction while maintaining a high service level of 0.761467 and reduces computational time to 82.32 s, highlighting its efficiency and adaptability. The innovation of this research lies in the application of the PCSO algorithm to dynamically manage inventory in real-time, aligning with sustainable inventory management practices. This study significantly contributes to the field by enhancing the economic and environmental sustainability of supply chain operations. Full article

Abstract: Existing studies need to pay more attention to the policy mechanisms of how to promote the development of cultural and creative industries through coordinating multi-stakeholder participation. Based on evolutionary game theory, this study constructs a three-party evolutionary game model to analyze the behavioral patterns of the government, cultural and creative enterprises, and consumers under different support policies. In addition, it takes the Chinese intangible cultural heritage of “Huaihe willow weaving” as a typical case for simulation. The results show that the probability of the system converging to the expected equilibrium point is low after implementing the certification policy, but the probability of the system converging to the expected equilibrium is significantly increased after implementing the incentive policy. Producer subsidies have significant advantages over consumer subsidies. At this stage, the government should consider jointly implementing the “certification + incentives” policy in phases to promote the development of cultural and creative industries. Full article

Physiological disorders impact the yield and quality of marketable fruit in tomato. Puffy fruit caused by cavities inside the locule can be problematic for processing and fresh market quality. In this paper, we used a recombinant inbred line (RIL) and three derived processing tomato populations to map and validate quantitative trait loci (QTLs) for fruit puffiness across environments. Binary interval mapping was used for mapping the incidence of fruit puffiness, and non-parametric interval mapping and parametric composite interval mapping were used for mapping severity. Marker–trait regressions were carried out to validate putative QTLs in subsequent crosses. QTLs were detected on chromosome (Chr) 1, 2, and 4. Only the QTL on Chr 1 was validated in progeny from subsequent crosses. This QTL explained up to 22.5% of the variance in the percentage of puffy fruit, with a significant interaction between loci on Chr 2 and 4, increasing the percentage of puffy fruit by an additional 15%. The allele responsible for puffy fruit on Chr 1 was inherited from parent FG02-188 and was dominant towards increased incidence and severity. Marker-assisted selection (MAS) for the QTL on Chr 1 was as efficient as genomic selection (GS) in reducing the incidence and severity of puffy fruit, despite the potential contribution of other loci. Full article

Agricultural irrigation requires significant consumption of freshwater resources and energy. The integration of photovoltaic power generation into irrigation systems has been extensively investigated in order to save the cost of energy. However, current research often neglects the coupling relationship between photovoltaic power generation and irrigation schemes. This study presented a novel smart integrated photovoltaic pump station system to effectively address the issue associated with water and energy consumption in irrigation. An optimization model was proposed to synchronize the energy consumption of irrigation pump stations with photovoltaic power generation, accurately meeting the irrigation water demand while maximizing solar energy utilization. The optimization model incorporates power balance, grid-connected power, and total water demand as constraints while considering pump speed as the decision variable and aiming to minimize daily operational costs. Finally, a high-standard farmland was used as a case study to validate the efficacy of the optimization strategy through two photovoltaic grid-connected policies—one allowing for the sale of surplus power and the other prohibiting it. An improved dynamic programming method was employed to solve for optimal energy consumption schemes under different water demand conditions; the results were compared against traditional methods, revealing potential cost savings ranging from 6.2% to 30.5%. The optimization model and method propose a new operational concept for the irrigation system with photovoltaic generation, effectively utilizing the distinctive features of both irrigation and photovoltaics to optimize water and energy resources. Full article

The use of validated tools to evaluate the nutritional status of the cancer patient provides guaranteed precision and reliability in their nutritional evaluation, ensuring that the information is accurate and reflects the patient's situation. The aim of this study was to identify the valid and reliable instruments in the evaluation of the nutritional status of cancer patients with a diagnosis of solid tumor undergoing antineoplastic treatment (chemotherapy and/or immunotherapy). A scoping review was conducted to search for original articles published in scientific journals in English, Spanish, or Portuguese in the past five years. In order to identify potentially relevant documents, searches were performed in the following databases: SCOPUS, WOS, CINAHL, MEDLINE, BVS, and PUBMED. DECS-MeSH descriptors and Boolean operators were used. In addition, the Arksey and O’Malley protocol, the Joanne Briggs Institute (JBI) method, and the flow chart of the Preferred Information Elements for Systematic Reviews and Meta-Analyses, known as PRISMA, were followed. The initial search strategy identified a total of 164 references, which were examined successively, leaving a final selection of ten studies. It was found that the most used instrument for nutritional evaluation was the Patient-Generated Subjective Global Assessment (PG-SGA). Other questionnaires also stood out such as the Mini Nutritional Assessment (MNA), the Malnutrition Universal Screening Tool (MUST), the Nutritional Risk Screening (NRS 2002), and the Functional Assessment of Anorexia/Cachexia Therapy (FAACT). The variation in the tools used ranges from subjective assessments to objective measurements, thus underlining the need for a comprehensive and individualized approach. Full article

Encephalitis and acute disseminated encephalomyelitis (ADEM) are often caused or triggered by viruses—but the specific pathogen commonly remains unidentified in routine care. We explored the use of viral metagenomic next-generation sequencing (mNGS) in addition to PCR testing of non-invasive stool samples to see if unbiased testing could potentially increase diagnostic yield. To identify specific clinical cases at the point of care, we took advantage of a previously published digital app allowing instant clinical case classification based on consensus case criteria, the VACC-Tool. This hospital-based prospective digital surveillance program assessed 100 pediatric patients (mean age: 11 years, range: 0.15–17.85; 49% male) with case-confirmed encephalitis and/or ADEM. Analysis of case classification at the point of care revealed that in routine care, 96% of confirmed encephalitis/ADEM cases had been missed. Overall agreement of routine care diagnoses with digital encephalitis/ADEM case classification was <50%. Also in routine care, only 13% of cases held a virus-related diagnosis, i.e., herpesvirus (n = 8) and enterovirus infection (n = 5). Use of mNGS increased the yield of virus detection by 77% (n = 23 virus hits). Specifically, mNGS identified 10 additional virus species beyond herpes- and enteroviruses. Of the additional 23 virus hits detected with mNGS, PCR confirmation was possible post hoc in 14 cases (61%). Linking digital case classification, mNGS, and PCR testing may not be feasible in routine care at this point but may help to provide hints to the pathogenesis of encephalitis/ADEM in childhood, warranting further research and exploration. Full article

(1) The study aims to utilize a reported approach for culturing mesophilic bacteria from a plastic waste environment; (2) The work revived mesophilic microbial population from an aged PET recycling site using a culture-based approach, and determined the purified isolates in genus level in 16S identification; (3) A total of 59 bacterial isolates were obtained, in which microbial species, including Pseudomonas spp, Rhodococcus spp, and Burkholderia spp were identified as abundance. It was observed that the surviving microbes favoured sodium propionate as a short-chain carbon source for growth, rather than the intended plastic substrate, PET. The preference of sodium propionate utilization by several bacterial isolates, including 5601W (detected as Rhodococcus spp.), 5601Y, 7801, and 7802 (detected as Burkholderia spp.), was confirmed through growth curve analysis and cell enumeration conducted in a medium where sodium propionate served as the sole carbon source.; (4) The microbial demonstration revealed the metabolic complex of microbial communities in the environment and indicated the challenges associated with bacterial isolation from environments with accumulated plastic waste. Full article

One of the most pressing challenges associated with SARS treatment is the emergence of new variants that may be transmissible, causing more severe disease or being resistant to the current standard of treatment. This study aimed to identify potential drug candidates from novel imidazole derivatives against SARS-CoV-2 main protease (Mpro), a crucial drug target for treating viral infection, using a computational approach that integrated molecular docking and dynamics simulation. In this study, we utilized AutoDock Vina within the PyRx workspace for molecular docking analysis to explore the inhibitory effects of the compounds on the Mpro, a drug target for SARS-CoV-2. The ADMET properties of these compounds, including absorption, distribution, metabolism, excretion, and toxicity, were evaluated using the SwissADME and ADMETLab servers. Each of the 18 compounds that were tested demonstrated strong binding affinities towards Mpro, with imidazolyl–methanone C10 showing the most significant binding affinity. Moreover, pyridyl–imidazole C5, thiophenyl–imidazole C1, and quinoline–imidazole C14 displayed binding affinities of −8.3, −8.2, and −7.7 Kcal/mol, respectively. These compounds interacted with specific amino acid residues (HIS A:41—CYS A:145) within the Mpro protein. To assess the stability of the ligand with the best binding affinity, molecular dynamics (MD) simulations were conducted using Schrodinger software, which revealed its stability over the simulation period. The study provides valuable insights into the potential of imidazole derivatives as SARS-CoV-2 Mpro inhibitors. All compounds including C10 display promising characteristics and hold potential as drug candidates for SARS-CoV-2. However, further optimization and experimental validation of these compounds are necessary to advance their development as effective therapeutics against viral infections. Full article

The objective of this qualitative investigation is to identify the assistive technology recognized by students with disabilities and to determine the assistive technology (software apps and devices) they require both at university and at home. A total of forty-two students, comprising 20 males and 22 females, were recruited from four different countries (Germany, Greece, Italy, and Spain) for participation in this study. The sample encompassed 10 students with visual impairments, 11 with hearing impairments, 11 with mobility impairments, and 10 with specific learning disabilities. Semi-structured interviews were conducted with the students either online or in person. Content analysis was employed to scrutinize the data obtained from these interviews. The outcomes of this analysis shed light on the assistive technology acknowledged, utilized, or desired by students with disabilities in both academic and domestic settings. The findings from this study carry practical implications for fostering inclusive and accessible education within higher education institutions, benefiting accessibility units/offices staff as well as teaching personnel. Full article

Babesiosis, caused by Babesia ovis, is a major seasonal issue in sheep, particularly in countries like Türkiye with high Rhipicephalus bursa tick populations. Previous studies employing various methods such as microscopy, serology, or molecular techniques have reported different epidemiological data concerning ovine babesiosis. Addressing this knowledge gap, our study employed a combined nested PCR (nPCR)/indirect ELISA (iELISA) approach, analyzing blood samples collected from 414 sheep between April and July 2023 using both techniques. nPCR amplified the 18S ribosomal RNA gene of B. ovis and determined a molecular prevalence of 1.9%. Conversely, serological testing using iELISA targeted the BoSA1 antigen and revealed a significantly higher positivity rate of 59.9% for anti-B. ovis antibodies. The temporary presence of Babesia after recovery reduces nPCR sensitivity, resulting in lower molecular prevalence. However, even if Babesia is not present in the host, anti-B. ovis antibodies remain in the serum for a long time and can be detected serologically. Our study underscores the necessity of concurrently employing molecular and serological methods for an accurate assessment of B. ovis prevalence. It highlights the importance of comprehensive epidemiological approaches for effective disease management in sheep populations. Full article

The four-wheel steering distributed drive vehicle is a novel type of vehicle with independent control over the four-wheel angle and wheel torque. A method for jointly controlling the distribution of the wheel angle and torque is proposed based on this characteristic. Firstly, the two-degrees-of-freedom model and ideal reference model of four-wheel steering vehicle are established; then, the four-wheel steering controller and torque distribution controller are designed. The rear wheel angle is controlled by the feedforward controller and the feedback controller. The feedforward controller takes the side slip angle of the center of mass as the control target, and the feedback controller takes the yaw angle as the control target. Torque is controlled by two control layers, the additional yaw moment of the upper layer is calculated by the vehicle motion state and fuzzy control theory, and the lower layer distributes wheel torque through the road adhesion coefficient and wheel load. Finally, a simulation platform is established to verify the effectiveness of the proposed control algorithm. Full article

This study explores the interrelationship between community attachment in AlUla Heritage City (located in Saudi Arabia) and tourists’ green consumption practices, testing support for green tourism development as a mediator. The old historical city of AlUla, a significant city experiencing ongoing preservation and tourism development, represents an adequate context for exploring the link between community attachment and green tourism practices. This study employs a quantitative approach, including surveys with 328 local residents of AlUla. A structural equation modeling partial least square (PLS-SEM) analysis is conducted to explore the indirect influence of community attachment on tourists’ green consumption through the mediating role of support for green tourism development. The findings indicated a positive path from community attachment in AlUla to tourists’ tendencies toward green consumption behavior. Moreover, the mediating effects of support for green tourism development suggested that a deep sense of community attachment improves tourists’ support for green practices in the tourism sector. This study adds to the extended body of the literature on place attachment and green tourism by emphasizing the significance of community place attachment in stimulating tourists’ green consumption practices. Several practical implications for policymakers seeking to promote green tourism practices in heritage cities like AlUla are explored from the study results. Full article

The burgeoning significance of antiferroelectric (AFE) materials, particularly as viable candidates for electrostatic energy storage capacitors in power electronics, has sparked substantial interest. Among these, lead-free sodium niobate (NaNbO₃) AFE materials are emerging as eco-friendly and promising alternatives to lead-based materials, which pose risks to human health and the environment, attributed to their superior recoverable energy density and dielectric breakdown strength. This review offers an insightful overview of the fundamental principles underlying antiferroelectricity and the applications of AFE materials. It underscores the recent advancements in lead-free NaNbO₃-based materials, focusing on their crystal structures, phase transitions, and innovative strategies devised to tailor their electrostatic energy storage performance. Finally, this review delineates the prevailing challenges and envisages future directions in the realm of NaNbO₃-based electrostatic energy storage capacitors, with the goal of fostering further advancements in this pivotal field. Full article