Platelets play essential roles in the formation of blood clots by clumping with coagulation factors at the site of vascular injury to stop bleeding; therefore, a reduction in the platelet number or disorder in their function causes bleeding risk. In our research, we developed a method to assess platelet aggregation using an optical approach within a microfluidic chip’s channel by evaluating the size of laser speckles. These speckles, associated with slowed blood flow in the microfluidic channel, had a baseline size of 28.54 ± 0.72 µm in whole blood. Removing platelets from the sample led to a notable decrease in speckle size to 27.04 ± 1.23 µm. Moreover, the addition of an ADP-containing agonist, which activates platelets, resulted in an increased speckle size of 32.89 ± 1.69 µm. This finding may provide a simple optical method via microfluidics that could be utilized to assess platelet functionality in diagnosing bleeding disorders and potentially in monitoring therapies that target platelets. Full article

Background and Objectives: This study explored how nefopam, a non-opioid analgesic in a multimodal regimen, impacts postoperative pain, opioid use, and recovery quality in single-port robot-assisted laparoscopic cholecystectomy (RALC) patients with a parietal pain block, addressing challenges in postoperative pain management. Materials and Methods: Forty patients scheduled for elective single-port RALC were enrolled and randomized to receive either nefopam or normal saline intravenously. Parietal pain relief was provided through a rectus sheath block (RSB). Postoperative pain was assessed using a numeric rating scale (NRS) in the right upper quadrant (RUQ) of the abdomen, at the umbilicus, and at the shoulder. Opioid consumption and recovery quality, measured using the QoR-15K questionnaire, were also recorded. Results: The 40 patients had a mean age of 48.3 years and an average body mass index (BMI) of 26.2 kg/m². There were no significant differences in the pre- or intraoperative variables between groups. Patients receiving nefopam reported significantly lower RUQ pain scores compared to the controls, while the umbilicus and shoulder pain scores were similar. Rescue fentanyl requirements were lower in the nefopam group in both the PACU and ward. The QoR-15K questionnaire scores for nausea and vomiting were better in the nefopam group, but the overall recovery quality scores were comparable between the groups. Conclusions: Nefopam reduces RUQ pain and opioid use post-single-port RALC with a parietal pain block without markedly boosting RSB’s effect on umbilicus or shoulder pain. It may also better manage postoperative nausea and vomiting, underscoring its role in analgesia strategies for this surgery. Full article

Oil transportation becomes much more complicated due to the solidification of paraffins in them at low temperatures and the resulting increase in oil viscosity. To solve this problem, special additives as pour point depressants (PPDs) are used to prevent the agglomeration of paraffin crystals. In this work, 15 PPDs were obtained and tested, consisting of a solution of polyethylene in hexane and also, in some cases, from magnetic nanoparticles (MNPs) extracted from coal fly ash. The most effective result was observed with a mixture of 0.25% polyethylene in hexane and 2% MNPs, which managed to lower the oil’s pour point from 18 °C to −17 °C. Full article

Given that Ecuadorians are one of the largest groups of Hispanics living in New York, they have become a tight community that they now call little Ecuador. Although Ecuadorians living in the diaspora in NYC come from different parts of the country (mostly from the Andean region), they share the same cultural practices they performed in Ecuador that give them the sense of being in their country without bearing the instability and turmoil their country experiences. This shows how the group has fostered a sense of a multifaceted, multidimensional simultaneity between the host country and the motherland. The goal of this paper is to analyze the strategies Ecuadorian migrants use to validate their language and cultural practices to negotiate their identity as a group. Data for this paper come from ethnographic observations, semi-spontaneous conversations, oral interviews with members of the group, along with pictures taken while walking the community and participating in some of their events. Our study reveals that participants hold varying perceptions regarding their linguistic and cultural practices. However, it is noteworthy that they recognize these practices as a manifestation of Ecuadorianness, signifying a sense of solidarity among community members. Full article

Osteoarthritis is a degenerative joint disease caused by the wear and tear of joint cartilage. The definitive and resolving treatment is prosthetic replacement of the articular surface, the demand of which is on the rise for patients with mild to moderate severity. However, a conservative strategy may be considered that aims to reduce and contain pain symptoms by postponing surgical treatment in the case of worsening that can no longer be otherwise controlled. Intra-articular infiltrations, like other therapeutic strategies, are not without complications, and among these the most feared is joint infection, especially in anticipation of future prosthetic replacement. Is important to avoid periprosthetic joint infections because they represent one of the third most common reasons for revision surgery. Using cases found in the literature, the aim of this article is to determine if there is a real correlation between the type of injections, the number of doses injected and the time between infiltrations and the surgical procedure. Full article

This study aimed to determine whether there is a difference between the levels of imagery and mental toughness in the context of sports performance in male and female athletes. A total of 344 track and field athletes, 205 male (59.6%, 23.3 ± 4.0 years) and 139 female (40.4%, 22.9 ± 4.0 years), voluntarily participated in the study. Imagery Inventory and Mental Toughness Inventory in Sport were used as data collection tools in the study. In the evaluation of athletic performance, athletes were asked about their ranks in the years 2020, 2021, and 2022 and were categorized according to the scoring tables specified by the International Association of Athletics Federation. A MANOVA analysis was used to determine whether there was a difference between low (−2% to +5%), medium (+6 to +11%), and high (+12 to +17%) performers among male and female athletes, and a post hoc analysis was used to determine the source of the difference. According to the present findings, there was no significant difference between the imagery and mental toughness levels of athletes with high, medium, and low performance among male athletes. On the contrary, a significant difference was detected between the imagery and mental toughness levels of female athletes with medium and high performances, showing that athletes in the high-performance range had higher levels of imagery (Eta² = 8) and mental toughness (Eta² = 10) than athletes in the medium- and low-performance ranges. The findings of this study show that imagery and emotional intelligence are important factors for sports performance. In this context, coaches and sports psychologists can include these parameters in their training programs to achieve the optimal performance range. Full article

Objectives: Ophthalmic three-dimensional (3D) digital surgery can reproduce high-definition surgical images; however, 3D digital surgery is limited by recording capacities. We examined the relationship between the minimum image quality required to reproduce surgical images and recording capacity. Methods: Patients who underwent simultaneous vitrectomy and cataract surgery by the same surgeon using a 3D digital surgery system at Juntendo University Urayasu Hospital between February and October 2021 were evaluated. Various quality (Q) and frame rate (FR) settings were used for each case. Four vitreous surgeons evaluated the reproducibility of recorded images of macular manipulation for epiretinal membrane (ERM) and macular hole (MH) cases and those of peripheral retinal manipulation for rhegmatogenous retinal detachment (RRD) cases. The video bitrate and minimum settings required to reproduce surgical images and factors affecting surgical image reproducibility were examined. Results: A total of 129 eyes of 129 patients were observed. The minimum image quality required to reproduce surgical images was 11.67 Mbps. The Q and FR for periretinal processing and Q for macular manipulation affected surgical image reproducibility (p = 0.025, p = 0.019, and p = 0.07, respectively). The minimum recording settings required to obtain highly reproducible images were Q = 3 and FR = 40. The total file size for vitrectomy video recordings with these settings was as compact as 3.17 GB for 28 min. Conclusions: During 3D digital surgery, highly reproducible surgical images can be obtained with a small storage capacity using settings of at least Q = 3 and FR = 40. Full article

This research focuses on addressing a significant concern in the aviation industry, which is drag. The primary objective of this project is to achieve drag reduction through the implementation of riblets on a wing featuring the NACA 2412 aerofoil, operating at subsonic speeds. Riblets, with the flow direction on wing surfaces, have demonstrated the potential to effectively decrease drag in diverse applications. This investigation includes computational analysis within the ANSYS Workbench framework, employing a polyhedral mesh model. The scope of this research encompasses the analysis of both a conventional wing and a modified wing with riblets. A comparative analysis is conducted to assess variations in drag values between the two configurations. Parameters, including geometry, dimensions, and riblet placement at varying angles of attack, are explored to comprehend their impact on drag reduction. Notably, 15.6% and 23% reductions in drag were identified at a 16-degree angle of attack with midspan and three-riblet models, separately. The computational mesh and method were validated using appropriate techniques. Full article

We assessed the effect of non-protein thiols (NPSH), reduced glutathione (GSH) and n-acetylcysteine (NAC), on resin shear bond strength (SBS) to hydrogen peroxide (H₂O₂)-treated dentin, and their effects on the characteristics of dentin in comparison to ascorbic acid (AA) and sodium thiosulfate (STS). H₂O₂-treated dentin was conditioned with 5% AA, GSH, NAC, or STS applied for 1 or 5 min. The positive control group received H₂O₂ without antioxidant application, and the first negative control group received distilled water (DW). The specimens received resin bonding immediately after treatment except for the second negative control group (delayed bonding). Microhardness, roughness, and topography were studied. The SBS values of all antioxidants were statistically greater than the positive control group (p < 0.05); however, NAC and AA applied for 1 min demonstrated the highest values, which were comparable to delayed bonding. All treatments removed the smear layer except DW, H₂O₂, and STS. The negative effect of H₂O₂ on resin–dentin bonding was mitigated by the application of the antioxidants; however, their efficiencies were dependent on the antioxidant type and time of application. NAC was more effective in optimizing resin bonding to bleached dentin compared to GSH at 1 min application and STS at both application times but was comparable to AA. Negligible negative effects on the substrate’s roughness and microhardness were detected. The antioxidant properties of the agent and its capacity to remove the smear layer are the processes underpinning the ability of a certain antioxidant to reverse the effect of H₂O₂ on bonding. Full article

Although the incidence of infections in orthopedic surgeries, including periprosthetic surgeries, remains low at approximately 1–2%, the number of surgeries and the incidence of drug-resistant bacteria is increasing. The cost and morbidity associated with revision surgeries are huge. More effective drug combinations and delivery methods are urgently needed. In this paper, three anti-infective drugs (vancomycin, rifampicin, and silver sulfadiazine) have been jointly and effectively electrospun in thin (0.1 mm) flexible nanofiber mats of either poly (methyl methacrylate) (PMMA) or poly (lactic-co-glycolic acid) (PLGA). The inclusion of poly (ethylene glycol) (PEG) enabled optimal drug release with a reduced water contact angle for wetting. The controlled release of these three agents from 20% PEG (w/w to polymer)-blended PMMA or PLGA nanofiber mats may allow for the prophylactical prevention of implant-related infections or provide methods to treat orthopedic infections at the time of revision surgeries. These combinations of drugs provide excellent additive or synergistic antibiotic action against a broader spectrum of bacteria than each drug alone. Full article

Selection into a County Age Group (CAG; under 10–18) programme is the first step for young aspiring cricketers on their journey to achieving professional status. Recognising that the British South Asian (BSA) community represents 30% of those who play recreational cricket compared to less than 5% of those who are selected to play at the professional level in England and Wales, it is important to better understand the characteristics of selected and non-selected players based on ethnicity to identify potential sociocultural differences during selection. Thus, the purpose of this study was to investigate the multidimensional factors that differentiated between selected and non-selected adolescent male cricketers as well as between White British (WB) and BSA selected players into a CAG programme. A total of 82 male participants aged between 14 and 17 years were included (mean = 15.3 ± 1.1 years: selected n = 33 and non-selected n = 49: WB n = 34, BSA n = 44, Other n = 4). In total, 104 characteristics were measured over nine tests, which were subsequently placed into five overarching factors: (a) physiological and anthropometrical, (b) perceptual–cognitive expertise, (c) psychological, (d) participation history, and (e) socio-cultural influences. A Bayesian binomial regression was performed in rSTAN using a weak normal prior to highlight differentiators in selection as well as differences between WB and BSA selected players. The results highlighted that athleticism, wellbeing and cohesion, the number of older brothers, and being born in birth quarters two and three were positively correlated with player selection into a CAG. Conversely, increases in psychological scores, a greater number of younger brothers and older sisters, as well as antisocial behaviour resulted in a reduced chance of player selection. Finally, several developmental factors (i.e., athleticism, wellbeing and cohesion, psychological distress, and levels of anti-social behaviour) differed based on ethnicity. These exploratory findings serve as a useful opening to highlight there are important differences to consider based on selection and ethnicity in CAG cricket. Full article

A 23-month-old neutered male dog of unknown ancestry presented with a history of progressive neurological signs that included anxiety, cognitive impairment, tremors, seizure activity, ataxia, and pronounced visual impairment. The clinical signs were accompanied by global brain atrophy. Due to progression in the severity of disease signs, the dog was euthanized at 26 months of age. An examination of the tissues collected at necropsy revealed dramatic intracellular accumulations of autofluorescent inclusions in the brain, retina, and cardiac muscle. The inclusions were immunopositive for subunit c of mitochondrial ATP synthase, and their ultrastructural appearances were similar to those of lysosomal storage bodies that accumulate in some neuronal ceroid lipofuscinosis (NCL) diseases. The dog also exhibited widespread neuroinflammation. Based on these findings, the dog was deemed likely to have suffered from a form of NCL. A whole genome sequence analysis of the proband’s DNA revealed a homozygous C to T substitution that altered the intron 3–exon 4 splice site of CLN6. Other mutations in CLN6 cause NCL diseases in humans and animals, including dogs. The CLN6 protein was undetectable with immunolabeling in the tissues of the proband. Based on the clinical history, fluorescence and electron-microscopy, immunohistochemistry, and molecular genetic findings, the disorder in this dog was classified as an NCL resulting from the absence of the CLN6 protein. Screening the dog’s genome for a panel of breed-specific polymorphisms indicated that its ancestry included numerous breeds, with no single breed predominating. This suggests that the CLN6 disease variant is likely to be present in other mixed-breed dogs and at least some ancestral breeds, although it is likely to be rare since other cases have not been reported to date. Full article

Sociolinguistic frameworks of race have not been widely applied to non-Latinx Spanish learners in the United States. Consequently, there is limited insight into the impact of race on different learners’ use of Spanish in their communities, including the local or national raciolinguistic dynamics between Latinxs and non-Latinxs that inform these outcomes. This article examines ethnographic interviews of women recounting interactions and experiences of using Spanish in different U.S. contexts. In their accounts, I identify three racialized sociolinguistic processes central to their Spanish learning journeys, which I title racialized positionality, racialized embodiment, and indexical fields of racialization. These processes highlight, respectively, (1) the raciolinguistic dynamics enacted by Spanish language usage in the U.S. by non-Latinxs, (2) the sociolinguistic input a learner comes across as raciogendered subjects, and (3) the social meaning they ascribe to racialized terms and discourses in Spanish. As critical approaches to Spanish language learning and pedagogy continue to emerge, more research is warranted on these three processes to trace the impact of race on Spanish language learning and use. Full article

Al_0.6CoFeNi₂V_0.5 high entropy alloy was successfully designed and prepared via the nonconsumable arc-melting process, and it was annealed at 600 °C, 800 °C, and 1000 °C for 4 h. Its microstructure and mechanical properties were studied. The as-cast alloy consisted of FCC and BCC phases, and no phase transformation occurred during annealing at 600 °C. Hard Al₃V-type metal compounds precipitated during annealing at 800 °C, and BCC particles precipitated in the FCC matrix during annealing at 1000 °C. After annealing, the strength and hardness of Al_0.6CoFeNi₂V_0.5 high-entropy alloy both showed a decreasing trend, because the annealing process eliminated the internal stress in this alloy. However, as the annealing temperature increased, the strength and hardness of the Al_0.6CoFeNi₂V_0.5 high-entropy alloy samples gradually increased. This is because the hard Al₃V metal compounds precipitated when the annealing temperature was 800 °C, which produced the “second phase strengthening” effect. At 1000 °C, the larger volume fraction of the hard and fine BCC phase (21.81%) diffusely precipitated; the precipitation of this BCC phase not only produced a “second phase strengthening” effect, which also resulted in “solid solution strengthening”, ultimately exhibiting enhanced hardness and strength. These findings have important theoretical reference value for the study of the microstructure and mechanical properties of high-entropy alloys. And, this study plays a significant role in promoting the research and development of new component materials that bear compressive loads, such as columns in large factory buildings, supports for cranes, and clamping bolts for rolling mills in practical mechanical engineering. Full article

Adolescents with attention deficit hyperactivity disorder (ADHD) face significant driving challenges due to deficits in attention and executive functioning, elevating their road risks. Previous interventions targeting driving safety among this cohort have typically addressed isolated aspects (e.g., cognitive or behavioral factors) or relied on uniform solutions. However, these approaches often overlook this population’s diverse needs. This study introduces the “Drive-Fun” innovative intervention (DFI), aimed at enhancing driving skills among this vulnerable population. The intervention was tested in a pilot study including 30 adolescents aged 15–18, comparing three groups: DFI, an educational intervention, and a control group with no treatment. Assessments included a driving simulator, EEG, and Tobii Pro Glasses 2. Evaluation was conducted pre- and post-intervention and at a 3-month follow-up. Results indicated that the DFI group significantly improved in the simulated driving performance, attentional effort, and focused gaze time. The findings underscore that holistic strategies with personalized, comprehensive approaches for adolescents with ADHD are particularly effective in improving driving performance. These outcomes not only affirm the feasibility of the DFI but also highlight the critical role of sensor technologies in accurately measuring and enhancing simulator driving performance in adolescents with ADHD. Outcomes suggest a promising direction for future research and application. Full article

The objectives of this paper are to investigate the trade-offs between a physically constrained neural network and a deep, convolutional neural network and to design a combined ML approach (“VarioCNN”). Our solution is provided in the framework of a cyberinfrastructure that includes a +newly designed ML software, GEOCLASS-image (v1.0), modern high-resolution satellite image data sets (Maxar WorldView data), and instructions/descriptions that may facilitate solving similar spatial classification problems. Combining the advantages of the physically-driven connectionist-geostatistical classification method with those of an efficient CNN, VarioCNN provides a means for rapid and efficient extraction of complex geophysical information from submeter resolution satellite imagery. A retraining loop overcomes the difficulties of creating a labeled training data set. Computational analyses and developments are centered on a specific, but generalizable, geophysical problem: The classification of crevasse types that form during the surge of a glacier system. A surge is a glacial catastrophe, an acceleration of a glacier to typically 100–200 times its normal velocity. GEOCLASS-image is applied to study the current (2016-2024) surge in the Negribreen Glacier System, Svalbard. The geophysical result is a description of the structural evolution and expansion of the surge, based on crevasse types that capture ice deformation in six simplified classes. Full article

The aim of the article is to inventory gestures related to mockery, insult, attracting good luck, or warding off bad luck that a group of informants from Barcelona have performed. The data come from the application of the survey from the Atlas de Gestos, whose task is to collect gestures from the Pan-Hispanic context to describe the gestural repertoire of each territory and conduct comparative studies. The results, interpreted based on social factors (gender, age, and level of instruction), confirm the wide range of gestures for different functions (10 for mockery, 11 for insult and attracting good luck, and 9 for warding off bad luck). Regarding differences based on social variables, the results allow for indicating trends, namely, while the most used gesture to express mockery is sticking out the tongue, young people prefer the gesture of pointing and laughing, and informants over 55 years old only prefer laughing. Concerning insults, the use of peineta is widespread, although differences in behavior between men and women are observed. As for attracting good luck, Barcelona informants opt for crossing fingers, although older generations use gestures with more religious connotations, such as clasping hands. Finally, to ward off bad luck, Barcelona locals mention the gesture of crossing made with the index fingers of each hand. Full article

The Mediterranean diet well known for its beneficial health effects, including mood enhancement, is characterised by the relatively high consumption of extra virgin olive oil (EVOO), which is rich in bioactive phenolic compounds. Over 200 phenolic compounds have been associated with Olea europaea, and of these, only a relatively small fraction have been characterised. Utilising the OliveNet^TM library, phenolic compounds were investigated as potential inhibitors of the epigenetic modifier lysine-specific demethylase 1 (LSD1). Furthermore, the compounds were screened for inhibition of the structurally similar monoamine oxidases (MAOs) which are directly implicated in the pathophysiology of depression. Molecular docking highlighted that olive phenolics interact with the active site of LSD1 and MAOs. Protein–peptide docking was also performed to evaluate the interaction of the histone H3 peptide with LSD1, in the presence of ligands bound to the substrate-binding cavity. To validate the in silico studies, the inhibitory activity of phenolic compounds was compared to the clinically approved inhibitor tranylcypromine. Our findings indicate that olive phenolics inhibit LSD1 and the MAOs in vitro. Using a cell culture model system with corticosteroid-stimulated human BJ fibroblast cells, the results demonstrate the attenuation of dexamethasone- and hydrocortisone-induced MAO activity by phenolic compounds. The findings were further corroborated using human embryonic stem cell (hESC)-derived neurons stimulated with all-trans retinoic acid. Overall, the results indicate the inhibition of flavin adenine dinucleotide (FAD)-dependent amine oxidases by olive phenolics. More generally, our findings further support at least a partial mechanism accounting for the antidepressant effects associated with EVOO and the Mediterranean diet. Full article

Accurately identifying the Bottom Simulating Reflector (BSR) is a crucial and fundamental task in seismic exploration and the interpretation of gas hydrates in marine areas. During our seismic interpretation and inference work on a gas hydrate survey in the Qiongdongnan Sea area, we encountered a phenomenon that closely resembled the seismic reflection characteristics of BSR. By comparing and identifying various geological phenomena, we have determined that this unique seismic reflection phenomenon is, in fact, the reflection of the depositional bottom interface known as “mass transport deposits (MTDs)” as described by previous researchers. The physical properties of the MTDs developed on the shallow surface of the seafloor are similar to those of gas hydrate reservoirs in the seismic exploration of marine areas, particularly in the northern South China Sea’s Qiongdongnan Sea area. Due to the lack of active neotectonic movement in the area, most identified BSR reflection occurrences are parallel to the seafloor. Consequently, during the process of seismic interpretation of BSR in the Qiongdongnan Sea area, it may be confused with the bottom boundary reflection interface of MTDs. Accurately identifying MTDs’ sedimentary bodies in gas hydrate exploration activities in this area would greatly enhance the accurate identification of BSR and support the refined evaluation of gas hydrate resources. In this paper, the structural characteristics of MTDs are compared with the reflection characteristics of seismic profiles, the reflectors are identified as MTDs rather than BSR through analysis and interpretation, and the possible mechanism of hydrate accumulation in this region is discussed. Full article

Artificial insemination in queen honey bees is the only tool that provides complete control over mating for research and breeding purposes, making it essential in genetic improvement and conservation programs in this species. The aims of this study were to characterize drone semen bacterial loads by culture-dependent and independent methods and to describe their variation depending on the method of semen collection, the colony and the apiary. In the first experiment, the bacterial loads of semen collected from the seminal vesicles or from ejaculates was studied using culture-dependent methods. The collection method had a significant influence on the overall bacterial count in semen. Out of the 42 semen samples analyzed, 26 (61.9%) tested positive for bacterial isolation. This encompassed the entirety of samples obtained from the seminal vesicles (21 of 21), whereas only 23.8% of those derived from ejaculates (5 out of 21) showed bacterial isolation. In the second experiment, next-generation sequencing techniques were used to describe the microbiome of ejaculated drone semen for the first time. The most abundant phyla were Proteobacteria, Firmicutes, Bacteroidota and Actinobacteriota, while the most abundant genera were Lactobacillus, Staphylococcus, Prevotella, Alloprevotella and Streptococcus. The results showed that the apiary had a significant effect on the community structure composition and abundance of the seminal microbiota, and significative differences in abundance were observed for the genera Sphingomonas, Methylobacterium-Methylorubrum, Bifidobacterium and Alloprevotella. Significant differences were also observed in the richness of the microbiota between apiaries and colonies. Full article

Transformational leadership has been proposed as an approach that can inspire effective change. How this is manifest in schools is understudied in Irish primary schools, which have undergone significant change in recent years. The focus of this qualitative research study was primary school and system leaders’ knowledge of transformational school leadership, perceived benefits, limitations, and feasibility, and how transformational school leadership actually manifests in practice. Participants were recruited using purposive sampling. In-depth interviews were carried out with principals, deputy and assistant principals, and former school inspectors, with the interviews aligned to the following research questions: (1) How can we characterize school and system leaders’ knowledge, understanding and perceptions regarding the feasibility of transformational school leadership? (2) How do transformational school-leadership behaviours manifest in primary-school settings? Data analysis yielded the following themes and sub-themes: (1) Understanding of transformational school leadership: (i) transformation, change and growth, (ii) relationships, (iii) vision, mission, and goals, (iv) leading; (2) Perceptions of feasibility of transformational school leadership: (i) realism, (ii) people and relationships, (iii) practical challenges; (3) Benefits of transformational school leadership: (i) aspiration, (ii) culture, (iii) motivation and modelling, (iv) school community, (v) delivering quality learning; (4) Limitations of transformational school leadership: (i) personality, (ii) pressure, (iii) slow process, (iv) unexpected variables; (5) Manifestations of transformational school-leadership: (i) idealised influence, (ii) inspirational motivation, (iii) individualised consideration, (iv) intellectual stimulation, (v) school development, (vi) improving curricular offerings. Participants’ positive disposition to transformational school leadership was encouraging and suggests the need for further research, specifically to examine potential synergies between transformational and distributed school leadership. Full article

We investigated the pharmacokinetic pathway of berberine and its metabolites in vitro, in Caco-2 cells, and in human participants following the administration of dihydroberberine (DHB) and micellar berberine (LipoMicel^®, LMB) formulations. A pilot trial involving nine healthy volunteers was conducted over a 24 h period; blood samples were collected and subjected to Ultra High-Performance Liquid Chromatography–High Resolution Mass Spectrometry (UHPLC-HRMS) analyses to quantify the concentrations of berberine and its metabolites. Pharmacokinetic correlations indicated that berberrubine and thalifendine follow distinct metabolic pathways. Additionally, jatrorrhizine sulfate appeared to undergo metabolism differently compared to the other sulfated metabolites. Moreover, berberrubine glucuronide likely has a unique metabolic pathway distinct from other glucuronides. The human trial revealed significantly higher blood concentrations of berberine metabolites in participants of the DHB treatment group compared to the LMB treatment group—except for berberrubine glucuronide, which was only detected in the LMB treatment group. Similarly, results from in vitro investigations showed significant differences in berberine metabolite profiles between DHB and LMB. Dihydroberberine, dihydroxy-berberrubine/thalifendine and jatrorrhizine sulfate were detected in LMB-treated cells, but not in DHB-treated cells; thalifendine and jatrorrhizine-glucuronide were detected in DHB-treated cells only. While DHB treatment provided higher blood concentrations of berberine and most berberine metabolites, both in vitro (Caco-2 cells) and in vivo human studies showed that treatment with LMB resulted in a higher proportion of unmetabolized berberine compared to DHB. These findings suggest potential clinical implications that merit further investigation in future large-scale trials. Full article