The 2023 MDPI Annual Report has
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15 pages, 6665 KiB  
Article
The Effect of Apple Vinegar Addition on the Quality and Shelf Life of Cooked Sausage during Chilling Storage
by Anna Okoń, Dorota Zielińska, Piotr Szymański, Anna Łepecka, Urszula Siekierko, Katarzyna Neffe-Skocińska, Monika Trząskowska, Katarzyna Kajak-Siemaszko, Barbara Sionek, Marcelina Karbowiak, Danuta Kołożyn-Krajewska and Zbigniew J. Dolatowski
Appl. Sci. 2024, 14(10), 4027; https://doi.org/10.3390/app14104027 - 9 May 2024
Abstract
As more and more consumers are becoming conscious of the safety and taste of meat products, the use of natural additives and innovative processing techniques has gained significant attention. Naturally fermented fruit vinegar is rich in organic acids and antioxidant phenolic compounds. In [...] Read more.
As more and more consumers are becoming conscious of the safety and taste of meat products, the use of natural additives and innovative processing techniques has gained significant attention. Naturally fermented fruit vinegar is rich in organic acids and antioxidant phenolic compounds. In addition, it contains amino acids, minerals, vitamins, and provitamin beta-carotene, and the presence of acetic acid bacteria may have a positive effect on consumer health. The study aimed to assess the impact of different concentrations of apple vinegar addition on the quality of cooked sausage, focusing on physicochemical parameters, including fatty acid profile and oxidative stability, as well as microbiological quality and sensory changes after production and during chilling storage. Four variants of sausage were prepared: C—sausage without apple vinegar; V1—sausage with 1% of apple vinegar; V3—sausage with 3% of apple vinegar; and V5—sausage with 5% of apple vinegar. All of the tests were carried out after production, as well as after 7 and 14 days of refrigeration storage. The addition of apple vinegar decreased the pH value and increased the oxidation-reduction potential and lipid oxidation in the samples V1, V3, and V5. The sausage with the 5% addition of apple vinegar (V5) was characterized by significantly more intensive brightness (parameter L* = 54.67) in comparison to the C sample (parameter L* = 52.78). The sausages that were tested showed good microbiological quality concerning the total number of microorganisms, lactic acid bacteria, and the absence of pathogenic bacteria. The addition of apple vinegar contributed to the reduction in the intensity of the cured meat flavor and the fatty flavor. Therefore, according to the results presented in this work, it can be concluded that 3% of vinegar is the optimal addition, which may be used in the next step of investigation, taking into account color formation abilities as well as microbiological quality and lipid oxidation processes. Full article
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15 pages, 1772 KiB  
Article
Level of Agreement in Subjective Selection of Gingival Colour
by Cristina Gómez-Polo, Javier Montero, Norberto Quispe, Javier Flores-Fraile, Maria Portillo Muñoz and Ana María Martín Casado
Appl. Sci. 2024, 14(10), 4025; https://doi.org/10.3390/app14104025 - 9 May 2024
Abstract
Background and Objectives: Primary outcome: To assess the level of agreement between the objective and subjective methods for recording gingival colour in each area of the gingiva. Secondary outcome: To compare performance of the subjective visual method of gingival colour selection by [...] Read more.
Background and Objectives: Primary outcome: To assess the level of agreement between the objective and subjective methods for recording gingival colour in each area of the gingiva. Secondary outcome: To compare performance of the subjective visual method of gingival colour selection by a male observer and a female observer. Materials and Methods: A chromatic study was conducted on a total of 101 participants, in five gingival zones, from the free gingival margin to the mucogingival line, using a SpectroShade Micro spectrophotometer for the objective method and 21 ad hoc ceramic gingival shade tabs for the subjective method. A man and a woman of the same age, with the same amount of clinical experience in dentistry, selected the tab that most resembled the colour of participants’ gingiva. The “chromatic error” was then assessed by calculating the colour difference (using the Euclidean and CIEDE2000 formulae) between the CIELAB coordinates of the shade tab selected and the objective coordinates of the gingiva. The unweighted Kappa coefficient was used to calculate the level of agreement between observers. Results: For the male observer, the mean chromatic errors varied between ΔEab* 10.3 and 13.1 units, while for the female observer, the mean errors varied between ΔEab* 11.1 and 12.8: these differences were not statistically significant. Similarly, no statistically significant differences were found between the mean chromatic errors for the five gingival zones in either the male operator (p = 0.100) or the female operator (p = 0.093). The minimum level of agreement (unweighted Kappa) between the observers ranged from 0.1 to 0.4. Conclusions: Subjective selection of gingival colour was very inaccurate, by both the male observer and the female observer, for any area of the gingiva, with no differences identified between them. The level of agreement between the observers was low. These findings suggest that gingival colour should not be determined using solely subjective methods, given that the chromatic errors significantly exceeded the clinical acceptability threshold for gingiva (4.1 units for ΔEab* and 2.9 units for ΔE00). Both observers showed a tendency to select gingival shade tabs that were redder and bluer than the objective colours. Full article
(This article belongs to the Section Applied Dentistry and Oral Sciences)
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27 pages, 1645 KiB  
Article
Numerical Simulation and Modeling of Mechano–Electro–Thermal Behavior of Electrical Contact Using COMSOL Multiphysics
by Andrei Andras, Florin Dumitru Popescu, Sorin Mihai Radu, Dragos Pasculescu, Ildiko Brinas, Mirela Ancuta Radu and Daniela (Furdui) Peagu
Appl. Sci. 2024, 14(10), 4026; https://doi.org/10.3390/app14104026 - 9 May 2024
Abstract
Electrical contacts are important circuit components with diverse industrial applications, and their failure can lead to multiple unwanted effects. Hence, the behavior of electrical contacts is a widely studied topic in the scientific literature based on various approaches, tools, and techniques. The present [...] Read more.
Electrical contacts are important circuit components with diverse industrial applications, and their failure can lead to multiple unwanted effects. Hence, the behavior of electrical contacts is a widely studied topic in the scientific literature based on various approaches, tools, and techniques. The present study proposes a new approach to numerical modeling and simulation based on the Holm contact theory, aiming to study the dependence between the electric potential and the temperature within an electrical contact. Structured in five sections, the research was conducted using COMSOL Multiphysics software and its solid-state mechanics, electric current, and heat transfer modules in order to highlight contact behavior from mechanical, electrical and thermal points of view: the von Mises stress, contact force, electric field amplitude, variation of the electrical potential along the current path, temperature gradient, and dependence of temperature along the contact elements edges were obtained by simulation, and are graphically represented. The results show that the temperature increase follows a parabolic curve, and that for values higher than 4 mV of voltage drop, the temperature of the contact increases to 79.25 degrees (and up to 123.81 degrees for 5 mV) over the ambient temperature, thus the integrity of insulation can be compromised. These values are close (10–12%) to the analytically calculated ones, and also in line with research assessed in the literature review. Full article
(This article belongs to the Special Issue Recent Studies on Optimization in Electric Energy Systems)
20 pages, 7536 KiB  
Article
Fault-Tolerant Multiport Converter for Hybrid Distribution Systems: Configuration, Control Principles and Fault Analysis
by Simone Negri, Giovanni Ubezio and Roberto Sebastiano Faranda
Appl. Sci. 2024, 14(10), 4024; https://doi.org/10.3390/app14104024 - 9 May 2024
Abstract
Multiport converters (MCs) are widely adopted in many applications, from renewable energy sources and storage integration to automotive applications and distribution systems. They are used in order to interface different energy sources, storage devices and loads with one single, simple converter topology in [...] Read more.
Multiport converters (MCs) are widely adopted in many applications, from renewable energy sources and storage integration to automotive applications and distribution systems. They are used in order to interface different energy sources, storage devices and loads with one single, simple converter topology in contrast to the traditional approach, which can require different solutions made by two-port converters. MCs allow for a reduction in the number of components and cascaded conversion stages with respect to an equivalent system of two-port converters, resulting in reduced complexity, dimensions and costs, as well as in improved reliability and enhanced efficiency. Nevertheless, some aspects related to the design of MCs are still worth further discussion when MCs are applied to hybrid AC/DC distribution systems. First, most converters are developed for one specific application and are not modular in structure. Furthermore, many of the proposed solutions are not equally suitable for AC and DC applications and they can introduce significant issues in hybrid distribution systems, with earthing management being particularly critical. Even though most available solutions offer satisfying steady-state and dynamic performances, fault behavior is often not considered and the possibility of maintaining controllability during faults is overlooked. Building on these three aspects, in this paper, a new MC for hybrid distribution systems is presented. An innovative circuit topology integrating three-phase AC ports and three-wire DC ports and characterized by a unique connection between the AC neutral wire and the DC midpoint neutral wire is presented. Its control principles and properties during external faults are highlighted, and extensive numerical simulations support the presented discussion. Full article
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20 pages, 21419 KiB  
Article
A New Approach to Detect Hand-Drawn Dashed Lines in Engineering Sketches
by Raquel Plumed, Manuel Contero, Ferran Naya and Pedro Company
Appl. Sci. 2024, 14(10), 4023; https://doi.org/10.3390/app14104023 - 9 May 2024
Abstract
Sketched drawings sometimes include non-solid lines drawn as sets of consecutive strokes. They represent dashed lines, which are useful for various purposes. Recognizing such dashed lines while parsing drawings is reasonably straightforward if they are outlined with a ruler and compass but becomes [...] Read more.
Sketched drawings sometimes include non-solid lines drawn as sets of consecutive strokes. They represent dashed lines, which are useful for various purposes. Recognizing such dashed lines while parsing drawings is reasonably straightforward if they are outlined with a ruler and compass but becomes challenging when they are hand-drawn. The problem is manageable if the strokes are drawn consecutively so we can leverage the entire sequence. However, it becomes more challenging if they are drawn unordered, and/or we do not have access to the sequence (like in batch vectorization). In this paper, we describe a new approach to identify groups of strokes as depicting single hand-drawn dashed lines. The approach does not use sequence information and is tolerant with irregularities and imprecisions of the strokes. Our goal is to identify hidden lines of sketched engineering line-drawings, which would enable the interpretation of line-drawings with hidden edges, which currently cannot be efficiently vectorized. We speculate that other fields like hand-drawn graph interpretation may also benefit from our approach. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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25 pages, 3831 KiB  
Article
Predicting the Spread of a Pandemic Using Machine Learning: A Case Study of COVID-19 in the UAE
by Donthi Sankalpa, Salam Dhou, Michel Pasquier and Assim Sagahyroon
Appl. Sci. 2024, 14(10), 4022; https://doi.org/10.3390/app14104022 - 9 May 2024
Abstract
Pandemics can result in large morbidity and mortality rates that can cause significant adverse effects on the social and economic situations of communities. Monitoring and predicting the spread of pandemics helps the concerned authorities manage the required resources, formulate preventive measures, and control [...] Read more.
Pandemics can result in large morbidity and mortality rates that can cause significant adverse effects on the social and economic situations of communities. Monitoring and predicting the spread of pandemics helps the concerned authorities manage the required resources, formulate preventive measures, and control the spread effectively. In the specific case of COVID-19, the UAE (United Arab Emirates) has undertaken many initiatives, such as surveillance and contact tracing by introducing mobile apps such as Al Hosn, containment of spread by limiting the gathering of people, online schooling and remote work, sanitation drives, and closure of public places. The aim of this paper is to predict the trends occurring in pandemic outbreak, with COVID-19 in the UAE being a specific case study to investigate. In this paper, a predictive modeling approach is proposed to predict the future number of cases based on the recorded history, taking into consideration the enforced policies and provided vaccinations. Machine learning models such as LASSO Regression and Exponential Smoothing, and deep learning models such as LSTM, LSTM-AE, and bi-directional LSTM-AE, are utilized. The dataset used is publicly available from the UAE government, Federal Competitiveness and Statistics Centre (FCSC) and consists of several attributes, such as the numbers of confirmed cases, recovered cases, deaths, tests, and vaccinations. An additional categorical attribute is manually added to the dataset describing whether an event has taken place, such as a national holiday or a sanitization drive, to study the effect of such events on the pandemic trends. Experimental results showed that the Univariate LSTM model with an input of a five-day history of Confirmed Cases achieved the best performance with an RMSE of 275.85, surpassing the current state of the art related to the UAE by over 30%. It was also found that the bi-directional LSTMs performed relatively well. The approach proposed in the paper can be applied to monitor similar infectious disease outbreaks and thus contribute to strengthening the authorities’ preparedness for future pandemics. Full article
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25 pages, 3709 KiB  
Article
Data Acquisition, Processing, and Aggregation in a Low-Cost IoT System for Indoor Environmental Quality Monitoring
by Alberto Barbaro, Pietro Chiavassa, Virginia Isabella Fissore, Antonio Servetti, Erica Raviola, Gustavo Ramírez-Espinosa, Edoardo Giusto, Bartolomeo Montrucchio, Arianna Astolfi and Franco Fiori
Appl. Sci. 2024, 14(10), 4021; https://doi.org/10.3390/app14104021 - 9 May 2024
Abstract
The rapid spread of Internet of Things technologies has enabled a continuous monitoring of indoor environmental quality in office environments by integrating monitoring devices equipped with low-cost sensors and cloud platforms for data storage and visualization. Critical aspects in the development of such [...] Read more.
The rapid spread of Internet of Things technologies has enabled a continuous monitoring of indoor environmental quality in office environments by integrating monitoring devices equipped with low-cost sensors and cloud platforms for data storage and visualization. Critical aspects in the development of such monitoring systems are effective data acquisition, processing, and visualization strategies, which significantly influence the performance of the system both at monitoring device and at cloud platform level. This paper proposes novel strategies to address the challenges in the design of a complete monitoring system for indoor environmental quality. By adopting the proposed solution, one can reduce the data rate transfer between the monitoring devices and the server without loss of information, as well as achieve efficient data storage and aggregation on the server side to minimize retrieval times. Finally, enhanced flexibility in the dashboard for data visualization is obtained, thus enabling graph modifications without extensive coding efforts. The functionality of the developed system was assessed, with the collected data in good agreement with those from other instruments used as references. Full article
(This article belongs to the Special Issue Air Quality in Indoor Environments, 2nd Edition)
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16 pages, 628 KiB  
Article
Enhancing Botnet Detection in Network Security Using Profile Hidden Markov Models
by Rucha Mannikar and Fabio Di Troia
Appl. Sci. 2024, 14(10), 4019; https://doi.org/10.3390/app14104019 - 9 May 2024
Abstract
A botnet is a network of compromised computer systems, or bots, remotely controlled by an attacker through bot controllers. This covert network poses a threat through large-scale cyber attacks, including phishing, distributed denial of service (DDoS), data theft, and server crashes. Botnets often [...] Read more.
A botnet is a network of compromised computer systems, or bots, remotely controlled by an attacker through bot controllers. This covert network poses a threat through large-scale cyber attacks, including phishing, distributed denial of service (DDoS), data theft, and server crashes. Botnets often camouflage their activity by utilizing common internet protocols, such as HTTP and IRC, making their detection challenging. This paper addresses this threat by proposing a method to identify botnets based on distinctive communication patterns between command and control servers and bots. Recognizable traits in botnet behavior, such as coordinated attacks, heartbeat signals, and periodic command distribution, are analyzed. Probabilistic models, specifically Hidden Markov Models (HMMs) and Profile Hidden Markov Models (PHMMs), are employed to learn and identify these activity patterns in network traffic data. This work utilizes publicly available datasets containing a combination of botnet, normal, and background traffic to train and test these models. The comparative analysis reveals that both HMMs and PHMMs are effective in detecting botnets, with PHMMs exhibiting superior accuracy in botnet detection compared to HMMs. Full article
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17 pages, 502 KiB  
Article
Nice for Whom? A Dangerous, Not-So-Nice, Critical Race Love Letter
by G. T. Reyes
Educ. Sci. 2024, 14(5), 508; https://doi.org/10.3390/educsci14050508 - 9 May 2024
Abstract
In this article, I critically analyze and respond to empirical data in the form of racialized discourse—specifically, racist messages sent directly to me as a result of my previously published article entitled, “A Love Letter to Educational Leaders of Color: CREWing UP with [...] Read more.
In this article, I critically analyze and respond to empirical data in the form of racialized discourse—specifically, racist messages sent directly to me as a result of my previously published article entitled, “A Love Letter to Educational Leaders of Color: CREWing UP with Critical Whiteness Studies”. Being informed by a robust racial analysis of acts that reinforce white supremacy, this article will likely be perceived as not nice by those who benefit from and work to protect white supremacy. Likely, I will be the one accused of being hateful, divisive, and even racist. In order to interrogate the weaponization of this conception of “niceness”, my analysis will be driven by Critical Race Hermeneutics with white emotionality and whitelashing used as interpretive lenses. As this article’s engagement with these critical race frameworks poses a threat to those who benefit from racism, this is a dangerous, not-so-nice critical race love letter. Full article
(This article belongs to the Special Issue Niceness, Leadership and Educational Equity)
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13 pages, 3188 KiB  
Article
Longitudinal Assessment of BNT162b2- and mRNA-1273-Induced Anti-SARS-CoV-2 Spike IgG Levels and Avidity Following Three Doses of Vaccination
by Jimmie L. Bullock, Jr., Thomas E. Hickey, Troy J. Kemp, Jordan Metz, Sarah Loftus, Katarzyna Haynesworth, Nicholas Castro, Brian T. Luke, Douglas R. Lowy and Ligia A. Pinto
Vaccines 2024, 12(5), 516; https://doi.org/10.3390/vaccines12050516 - 9 May 2024
Abstract
SARS-CoV-2 vaccination-induced protection against infection is likely to be affected by functional antibody features. To understand the kinetics of antibody responses in healthy individuals after primary series and third vaccine doses, sera from the recipients of the two licensed SARS-CoV-2 mRNA vaccines were [...] Read more.
SARS-CoV-2 vaccination-induced protection against infection is likely to be affected by functional antibody features. To understand the kinetics of antibody responses in healthy individuals after primary series and third vaccine doses, sera from the recipients of the two licensed SARS-CoV-2 mRNA vaccines were assessed for circulating anti-SARS-CoV-2 spike IgG levels and avidity for up to 6 months post-primary series and 9 months after the third dose. Following primary series vaccination, anti-SARS-CoV-2 spike IgG levels declined from months 1 to 6, while avidity increased through month 6, irrespective of the vaccine received. The third dose of either vaccine increased anti-SARS-CoV-2 spike IgG levels and avidity and appeared to enhance antibody level persistence—generating a slower rate of decline in the 3 months following the third dose compared to the decline seen after the primary series alone. The third dose of both vaccines induced significant avidity increases 1 month after vaccination compared to the avidity response 6 months post-primary series vaccination (p ≤ 0.001). A significant difference in avidity responses between the two vaccines was observed 6 months post-third dose, where the BNT162b2 recipients had higher antibody avidity levels compared to the mRNA-1273 recipients (p = 0.020). Full article
(This article belongs to the Special Issue SARS-CoV-2 Variants, Vaccines, and Immune Responses)
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13 pages, 1551 KiB  
Article
Comparative Analysis of Vaccine-Induced Neutralizing Antibodies against the Alpha, Beta, Delta, and Omicron Variants of SARS-CoV-2
by Philipp Girl, Heiner von Buttlar, Enrico Mantel, Markus H. Antwerpen, Roman Wölfel and Katharina Müller
Vaccines 2024, 12(5), 515; https://doi.org/10.3390/vaccines12050515 - 9 May 2024
Abstract
The SARS-CoV-2 virus has infected more than 660 million people and caused nearly seven million deaths worldwide. During the pandemic, a number of SARS-CoV-2 vaccines were rapidly developed, and several are currently licensed for use in Europe. However, the optimization of vaccination regimens [...] Read more.
The SARS-CoV-2 virus has infected more than 660 million people and caused nearly seven million deaths worldwide. During the pandemic, a number of SARS-CoV-2 vaccines were rapidly developed, and several are currently licensed for use in Europe. However, the optimization of vaccination regimens is still ongoing, particularly with regard to booster vaccinations. At the same time, the emergence of new virus variants poses an ongoing challenge to vaccine efficacy. In this study, we focused on a comparative analysis of the neutralization capacity of vaccine-induced antibodies against four different variants of concern (i.e., Alpha, Beta, Delta, and Omicron) after two and three doses of COVID-19 vaccine. We were able to show that both two (prime/boost) and three (prime/boost/boost) vaccinations elicit highly variable levels of neutralizing antibodies. In addition, we did not observe a significant difference in antibody levels after two and three vaccinations. We also observed a significant decrease in the neutralization susceptibility of all but one SARS-CoV-2 variants to vaccine-induced antibodies. In contrast, a SARS-CoV-2 breakthrough infection between the second and third vaccination results in overall higher levels of neutralizing antibodies with a concomitant improved neutralization of all virus variants. Titer levels remained highly variable across the cohort but a common trend was observed. This may be due to the fact that at the time of this study, all licensed vaccines were still based exclusively on wild-type SARS-CoV-2, whereas infections were caused by virus variants. Overall, our data demonstrate the importance of (booster) vaccinations, but at the same time emphasize the need for the continued adaptation of vaccines to induce a protective immune response against virus variants in order to be prepared for future (seasonal) SARS-CoV-2 outbreaks. Full article
(This article belongs to the Special Issue Immune Effectiveness of COVID-19 Vaccines)
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12 pages, 3022 KiB  
Article
Dietary Replacement of Soybean Meal with Zanthoxylum bungeanum Seed Meal on Growth Performance, Blood Parameters, and Nutrient Utilization in Broiler Chickens
by Xing Chen, Yang Li, Aijuan Zheng, Zedong Wang, Xu Wei, Shuzhen Li, Adanan Purba, Zhimin Chen and Guohua Liu
Animals 2024, 14(10), 1420; https://doi.org/10.3390/ani14101420 - 9 May 2024
Abstract
Zanthoxylum bungeanum seed meal (ZBM), a novel plant protein raw material, has shown promising potential in enhancing the growth of broiler chickens as a substitute for soybean meal (SBM) in feed. In the artificial digestive experiment of vitro experiments, the digestibility of ZBM [...] Read more.
Zanthoxylum bungeanum seed meal (ZBM), a novel plant protein raw material, has shown promising potential in enhancing the growth of broiler chickens as a substitute for soybean meal (SBM) in feed. In the artificial digestive experiment of vitro experiments, the digestibility of ZBM and SBM were assessed using the SDS-III Single Stomach Animal Biometric Digestion System. Subsequently, 180 1-day old AA chicks were divided into three groups for in vivo experiments: corn–soybean-meal-based diet (CON group); ZBM replacing 5% soybean meal in the basal diet (ZBM-1 group); ZBM replacing 10% soybean meal in the basal diet (ZBM-2 group). The experiment period lasted for 42 days. Compared to SBM, ZBM demonstrated higher crude protein content, dry matter digestibility, and extracorporeal digestible protein. Compared with the CON group, the broilers in the ZBM-2 group showed improved ADG and ADFI during the 1–21 d, 22–42 d, and 1–42 d periods (p < 0.05). Furthermore, the ZBM groups exhibited significant increases in slaughter performance compared with the CON group (p < 0.05). The substitution of ZBM for SBM also leads to a significant reduction in serum enzyme indicators (p < 0.05). Additionally, the lipoprotein and total cholesterol of the ZBM groups were significantly lower than those of the CON group (p < 0.05). Substituting SBM with ZBM significantly enhances the activity of superoxide dismutase and the content of immunoglobulin G in broiler serum, while reducing the content of malondildehyde (p < 0.05). The ZBM groups showed significantly higher utilization of dry matter, crude protein, and energy compared with the CON group (p < 0.05). In conclusion, the study confirmed that the substitution of SBM with 5–10% ZBM in broiler diets has a significant positive effect on growth, development, antioxidant capacity, immune function, and nutrient utilization. This study not only provides a theoretical foundation for the utilization of ZBM in broiler diets but also offers an effective approach for reducing reliance on soybean meal. Full article
(This article belongs to the Section Animal Nutrition)
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15 pages, 13835 KiB  
Article
Wdr17 Regulates Cell Proliferation, Cell Cycle Progression and Apoptosis in Mouse Spermatocyte Cell Line
by Xin Zhao, Taili Jin, Xi Ji, Qiuyan Zhang, Xianyu Zhang, Zhenfang Wu, Zicong Li and Huaqiang Yang
Animals 2024, 14(10), 1418; https://doi.org/10.3390/ani14101418 - 9 May 2024
Abstract
We identified Wdr17 as a highly expressed gene in pachytene spermatocytes by transcriptomic analysis of mouse testis. Germ cell-deficient infertile mouse models had significantly reduced Wdr17 expression. We performed gene interference and overexpression in the mouse spermatocyte cell line GC-2spd(ts) and investigated how [...] Read more.
We identified Wdr17 as a highly expressed gene in pachytene spermatocytes by transcriptomic analysis of mouse testis. Germ cell-deficient infertile mouse models had significantly reduced Wdr17 expression. We performed gene interference and overexpression in the mouse spermatocyte cell line GC-2spd(ts) and investigated how Wdr17 affects spermatocyte growth and development. Our results showed that Wdr17 suppression significantly decreased cell growth rate and increased cell apoptosis in GC-2spd(ts) cells. Wdr17 suppression also arrested the cell cycle at the G1 phase. On the contrary, Wdr17 overexpression significantly promoted cell proliferation and inhibited cell apoptosis in GC-2spd(ts) cells. More cells were enriched at the S stage with a concomitant reduction of cells at the G1 stage. Wdr17 promotes mouse spermatocyte proliferation by advancing cell cycle progression and inhibiting cell apoptosis, indicating its potential role in regulating spermatogenesis in the mouse. Full article
(This article belongs to the Section Animal Reproduction)
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12 pages, 1564 KiB  
Article
Identification of Copy Number Variations and Selection Signatures in Wannan Spotted Pigs by Whole Genome Sequencing Data: A Preliminary Study
by Wei Zhang, Yao Jiang, Zelan Ni, Mei Zhou, Linqing Liu, Xiaoyu Li, Shiguang Su and Chonglong Wang
Animals 2024, 14(10), 1419; https://doi.org/10.3390/ani14101419 - 9 May 2024
Abstract
Copy number variation (CNV) is an important structural variation used to elucidate complex economic traits. In this study, we sequenced 25 Wannan spotted pigs (WSPs) to detect their CNVs and identify their selection signatures compared with those of 10 Asian wild boars. A [...] Read more.
Copy number variation (CNV) is an important structural variation used to elucidate complex economic traits. In this study, we sequenced 25 Wannan spotted pigs (WSPs) to detect their CNVs and identify their selection signatures compared with those of 10 Asian wild boars. A total of 14,161 CNVs were detected in the WSPs, accounting for 0.72% of the porcine genome. The fixation index (Fst) was used to identify the selection signatures, and 195 CNVs with the top 1% of the Fst value were selected. Eighty genes were identified in the selected CNV regions. Functional GO and KEGG analyses revealed that the genes within these selected CNVs are associated with key traits such as reproduction (GAL3ST1 and SETD2), fatty acid composition (PRKG1, ACACA, ACSL3, UGT8), immune system (LYZ), ear size (WIF1), and feed efficiency (VIPR2). The findings of this study contribute novel insights into the genetic CNVs underlying WSP characteristics and provide essential information for the protection and utilization of WSP populations. Full article
15 pages, 2908 KiB  
Article
Magnetic Resonance Imaging, Computed Tomographic and Radiographic Findings in the Metacarpophalangeal Joints of 31 Warmblood Showjumpers in Full Work and Competing Regularly
by Annamaria Nagy and Sue Dyson
Animals 2024, 14(10), 1417; https://doi.org/10.3390/ani14101417 - 9 May 2024
Abstract
There is a limited description of magnetic resonance imaging (MRI) and no information on computed tomographic (CT) findings in the fetlock of non-lame, non-racing sports horses. This study aimed to document comparative CT, MRI and radiographic findings in the metacarpophalangeal joints of showjumpers [...] Read more.
There is a limited description of magnetic resonance imaging (MRI) and no information on computed tomographic (CT) findings in the fetlock of non-lame, non-racing sports horses. This study aimed to document comparative CT, MRI and radiographic findings in the metacarpophalangeal joints of showjumpers in full work. Clinical and gait assessments, low-field MRI, fan-beam CT and radiographic examinations of both metacarpophalangeal joints were performed on 31 showjumpers. Images were analysed descriptively. In most limbs (53/62, 85.5%), there were CT and MRI changes consistent with densification in the sagittal ridge and/or condyles of the third metacarpal bone (McIII). Hypoattenuation (subchondral bone resorption) was seen in CT reconstructions in the metacarpal condyle dorsoproximally (n = 2) and dorsodistally (n = 1), in the sagittal groove (n = 2) and medial fovea (n = 1) of the proximal phalanx. The McIII resorptive lesions were detected on MR images but not the proximal phalanx lesions. None were identified on radiographs. In conclusion, MRI and CT abnormalities previously associated with lameness were seen in the front fetlocks of showjumpers without relevant lameness. Densification in the sagittal ridge and the metacarpal condyles likely reflects an adaptive change to exercise. Subchondral bone resorption may indicate an early stage of disease; follow-up information is needed to establish its clinical significance. Full article
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16 pages, 222 KiB  
Article
Veterinarians’ Self-Reported Behaviors and Attitudes toward Spectrum of Care Practices
by Emily D. Dolan and Margaret R. Slater
Animals 2024, 14(10), 1416; https://doi.org/10.3390/ani14101416 - 9 May 2024
Abstract
Access to veterinary care for animal owners is an important part of keeping animals healthy and keeping pets and people together whenever that is appropriate. Insufficient financial and other resources to allocate to veterinary care are major barriers for pet owners to receiving [...] Read more.
Access to veterinary care for animal owners is an important part of keeping animals healthy and keeping pets and people together whenever that is appropriate. Insufficient financial and other resources to allocate to veterinary care are major barriers for pet owners to receiving preventative, sick, and emergency services. The veterinary community has begun to incorporate offering a range of diagnostic and treatment options more intentionally in response to clients’ inability to pay and to a lesser extent to mitigate other barriers to care. Many veterinarians are nonetheless oriented toward providing specialized and more sophisticated care based on their training. This study sought to identify the self-reported behaviors, knowledge, attitudes, and beliefs of veterinarians about offering a spectrum of care options (SoC) to clients. The finding that many reported offering SoC is encouraging. However, veterinarians who report comfort and confidence in a variety of aspects of clinical care were most likely to offer SoC. Practitioners in the field for 20 or more years were less likely to offer SoC to clients with financial limitations. Rural veterinarians were more likely to offer SoC to any client compared to urban veterinarians. These results provide a point of reference and potential focus for veterinarians who are not currently offering SoC as well as an exploration of veterinarians’ reported knowledge, behaviors, attitudes, perceptions, and concerns about SoC. Full article
(This article belongs to the Section Companion Animals)
20 pages, 1277 KiB  
Article
How Power Affects Moral Judgments: The Presence of Harm to Life Modifies the Association between Power and Moral Choices
by Mufan Zheng, Ana Guinote and Wei Luo
Soc. Sci. 2024, 13(5), 256; https://doi.org/10.3390/socsci13050256 - 9 May 2024
Abstract
Lammers and Stapel reported that high power increases deontological (rule-based) moral thinking, and low power increases utilitarian (outcome-based) moral thinking. However, the dilemmas were mild and did not involve harm to life. Here, we examined whether the presence or absence of harm to [...] Read more.
Lammers and Stapel reported that high power increases deontological (rule-based) moral thinking, and low power increases utilitarian (outcome-based) moral thinking. However, the dilemmas were mild and did not involve harm to life. Here, we examined whether the presence or absence of harm to life affects the moral decisions of powerholders. To help establish the replicability and validity of the effects of power on moral judgments in the absence of harm to life, we first performed an exact replication of a study conducted by Lammers and Stapel, and this experiment was followed up by a similar study in an organizational context in China (Studies 1 and 2). Studies 3 and 4 investigated whether power and the presence/absence of harm to life interacted with preferences for deontological versus utilitarian moral judgments. Power consistently triggered deontological thinking. However, power differences in moral reasoning only emerged when there was no harm to life. Harm prompted deontological responses among control and powerless individuals, which nullified differences across the power conditions. The findings demarcate the generalizability of the association between power and a moral thinking style. Full article
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14 pages, 349 KiB  
Article
Enhancing Mental Health Predictions: A Gradient Boosted Model for Sri Lankan Camp Refugees
by Indranil Sahoo, Elizabeth Amona, Miriam Kuttikat and David Chan
Soc. Sci. 2024, 13(5), 255; https://doi.org/10.3390/socsci13050255 - 9 May 2024
Abstract
This study explores the mental health challenges encountered by Sri Lankan camp refugees, a population often marginalized in mental health research, and analyzes a range of factors including socio-demographic characteristics, living conditions in camps, and psychological variables. In quantitative mental health research, linear [...] Read more.
This study explores the mental health challenges encountered by Sri Lankan camp refugees, a population often marginalized in mental health research, and analyzes a range of factors including socio-demographic characteristics, living conditions in camps, and psychological variables. In quantitative mental health research, linear regression serves as a conventional approach for assessing the influence of diverse factors on mental health outcomes. However, this method fails to accommodate non-linear relationships between mental health variables and predictors and relies on stringent model assumptions that often do not align with real-world conditions. This study introduces a model-agnostic, advanced machine learning/artificial intelligence (ML/AI) technique, glmboost, as a viable alternative to linear regression. The glmboost algorithm is capable of fitting non-linear prediction models while also conducting variable selection. Moreover, the coefficients obtained from the glmboost model retain the same interpretability as those derived from linear regression. While the glmboost model identifies several key factors including post-migration living difficulties, post-traumatic stress disorder, difficulty in sleeping, poor family functioning, and lower informal support from families as markers of declining mental well-being among the Sri Lankan refugees, the linear regression overlooks vital predictors such as family functioning and family support, highlighting the importance of utilizing advanced ML/AI techniques like glmboost to comprehensively capture complex relationships between predictor variables and mental health outcomes among refugee populations. Thus, by introducing a novel, data-driven approach to mental health risk assessment, this study paves the way for more precise and efficient analyses and interventions in refugee settings, with the potential for improved resource allocation and personalized support, thus revolutionizing mental health service delivery in challenging environments. Additionally, it contributes to the academic discussion on refugee mental health while emphasizing the pivotal role of advanced data analytics in addressing complex health issues within humanitarian contexts. Full article
(This article belongs to the Section International Migration)
26 pages, 46539 KiB  
Article
Thermal Environment Analysis and Optimization for Large Space Buildings with Radiant Cooling Floors: A Case Study of Xianyang International Airport
by Rong Hu, Haolin Wang, Junqi Liang, Xiaoping Li, Wenheng Zheng and Gang Liu
Buildings 2024, 14(5), 1355; https://doi.org/10.3390/buildings14051355 - 9 May 2024
Abstract
Radiant cooling floors combined with ventilation systems have been widely applied in large space buildings. However, there has been a lack of research on system control strategies for their adaptation to weather changes. This study aimed to find control strategies for radiant cooling [...] Read more.
Radiant cooling floors combined with ventilation systems have been widely applied in large space buildings. However, there has been a lack of research on system control strategies for their adaptation to weather changes. This study aimed to find control strategies for radiant cooling floors combined with displacement ventilation systems used in large space buildings in order to achieve energy conservation and environmental improvement. Supply air temperature and cooling surface temperature were determined to be the control variables. It was found that cooling capacity of the combined system and the comfort index, PMV (predicted mean vote), were linear in relation to the supply air temperature and cooling surface temperature. The linear equations regarding cooling capacity and PMV were established separately using environment data, and then the optimal region was determined. A case study on Terminal 3 of Xi’an Xianyang International Airport was conducted. The thermal environment was investigated through on-site measurements, questionnaires, and numerical simulations with CFD (computational fluid dynamics). It was found that supply air temperature and cooling surface temperature had a significant impact on PMV, and less impact on the cooling capacity. Therefore, it was determined that the supply air temperature should be altered first when the indoor temperature exceeds the upper limit, and then the cooling surface temperature should be changed if the indoor environment continues to overheat with the supply air temperature set to 18 °C. Thus, the supply air temperature was kept at 18 °C, and the floor surface temperature was set to be 22 °C on a high-temperature day. The average PMV was 0.87, and the cooling capacity of the combined system was 200 W/(m2·K), according to the CFD simulation. In addition, the surface heat transfer coefficient of the cooling floor was found to be 10.26 W/(m2·K). This research provides important references for the design and operational management of radiant cooling floors in large space buildings. Full article
(This article belongs to the Special Issue Research on Energy Performance in Buildings)
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24 pages, 3998 KiB  
Article
Shaking Table Tests and Numerical Analysis Conducted on an Aluminum Alloy Single-Layer Spherical Reticulated Shell with Fully Welded Connections
by Jiawei Lu, Qiujun Ning, Xiaosong Lu, Fan Yang and Yuanshun Wang
Buildings 2024, 14(5), 1354; https://doi.org/10.3390/buildings14051354 - 9 May 2024
Abstract
Aluminum alloy offers the advantages of being lightweight, high in strength, corrosion-resistant, and easy to process. It has a promising application prospect in large-span space structures, with its primary application form being single-layer reticulated shells. In this study, shaking table tests were conducted [...] Read more.
Aluminum alloy offers the advantages of being lightweight, high in strength, corrosion-resistant, and easy to process. It has a promising application prospect in large-span space structures, with its primary application form being single-layer reticulated shells. In this study, shaking table tests were conducted on a 1/25 scale aluminum alloy single-layer spherical reticulated shell structure. A finite element (FE) model of the reticulated shell structure was established in Ansys. Compared with the experimental results, the deviation in natural frequency, acceleration amplitude, and displacement amplitude was less than 20%, confirming the validity of the model. An extensive analysis of the various rise–span ratios and connection constraints of a single-layer spherical reticulated shell structure was carried out using the proposed FE model. The experimental and simulation results showed that as the rise–span ratio of the aluminum alloy reticulated shell increases, the natural frequency of the reticulated shell structure also increases while the dynamic performance decreases. The connection of the circumferential members changes from a rigid connection to a hinged connection. The natural frequency of the reticulated shell structure is reduced by about 40% while the acceleration and displacement response values are decreased by approximately 15%. Full article
(This article belongs to the Section Building Materials, and Repair & Renovation)
19 pages, 1437 KiB  
Article
Effect of Micro-Cracks on Chloride Ion Diffusion in Concrete Based on Stochastic Aggregate Approach
by Qianfan Yang, Yuching Wu, Peng Zhi and Peng Zhu
Buildings 2024, 14(5), 1353; https://doi.org/10.3390/buildings14051353 - 9 May 2024
Abstract
For concrete structures in offshore areas, chloride ion erosion is one of the main factors affecting durability. It is crucial to evaluate the chloride ion permeability resistance of concrete structures. In this paper, a finite element simulation of the chloride ion diffusion process [...] Read more.
For concrete structures in offshore areas, chloride ion erosion is one of the main factors affecting durability. It is crucial to evaluate the chloride ion permeability resistance of concrete structures. In this paper, a finite element simulation of the chloride ion diffusion process in concrete is conducted. A mass diffusion finite element model based on a random aggregate approach is established to investigate the influences of an aggregate, the interface transition zone, and micro-cracks on the chloride ion diffusion coefficients in concrete. The results show that the mass diffusion finite element analysis based on the exponential function model and the power function model can effectively simulate the chloride ion diffusion process in concrete. In addition, the data reveal that volume fraction and distribution aggregates considerably affect chloride ion diffusivity in concrete. Also, the interface transition zone significantly accelerates chloride ion diffusion in concrete. Moreover, this acceleration effect exceeds the barrier effect of an aggregate. Full article
(This article belongs to the Special Issue Low-Carbon Concrete with Different Sources of Solid Waste)
16 pages, 1924 KiB  
Review
Code Requirements for the Seismic Design of Irregular Elevation RC Structures
by Davi Santos, José Melo and Humberto Varum
Buildings 2024, 14(5), 1351; https://doi.org/10.3390/buildings14051351 - 9 May 2024
Abstract
The recent seismic activity highlights the crucial need to enhance seismic design and safety assessment methods, particularly for irregular structures, in both new and existing constructions. The present study focuses on structural irregularities in elevation for buildings, as the design of structural systems [...] Read more.
The recent seismic activity highlights the crucial need to enhance seismic design and safety assessment methods, particularly for irregular structures, in both new and existing constructions. The present study focuses on structural irregularities in elevation for buildings, as the design of structural systems involves multiple variables that often result in irregularities in many buildings. This work aims to perform a comparative assessment of the criteria adopted for the evaluation of the structural irregularities in elevation present in European and international seismic codes. This paper is structured as follows: Firtsly, it discusses structural irregularities and more specifically the most common types of structural damage due to seismic events. Then, it shows the documented experiences of structural damages in seismic events associated with structural irregularities in China, Italy, Spain, Nepal and Mexico. Additionaly, it discusses the requirements of the standards on irregularities and their limitation in that matter. At the end of this section, the different approaches of each code in irregularities in elevation are compared. All assessed seismic codes addresses the structural irregularity issue, attributing the desired characteristics of a seismic-resistant structure. However, there are considerable development differences between norms, demonstrated on ambiguity of few codes on criteria of vertical irregularies. Full article
(This article belongs to the Special Issue Seismic Design of Building Structures)
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18 pages, 1736 KiB  
Article
Investigation of Surface Modification of Bagasse Fibers: Performance of Asphalt Binders/Mixtures with Bagasse Fibers
by Haiwei Xie, Yixuan Jia, Chunsheng Zhu, Weidong Liu, Zuzhong Li and Zhipeng Huang
Buildings 2024, 14(5), 1352; https://doi.org/10.3390/buildings14051352 - 9 May 2024
Abstract
The influence of surface modification on the properties of bagasse fibers and asphalt binders/mixtures was investigated. Bagasse fibers were modified by single, binary, and ternary methods with hydrochloric acid, sodium hydroxide, and sodium chlorite, respectively. The physical and chemical properties of bagasse fibers [...] Read more.
The influence of surface modification on the properties of bagasse fibers and asphalt binders/mixtures was investigated. Bagasse fibers were modified by single, binary, and ternary methods with hydrochloric acid, sodium hydroxide, and sodium chlorite, respectively. The physical and chemical properties of bagasse fibers were analyzed by scanning electron microscopy, Fourier transform infrared spectroscopy, and an adsorption test, respectively. The rheological properties of asphalt binders with bagasse fibers or lignin fibers were analyzed by the dynamic shear rheometer test and bending beam rheometer test. In addition, the performance of asphalt mixtures with bagasse fibers or lignin fibers were evaluated by a wheel rutting test, bending test at a low temperature, and water stability test, respectively. In conclusion, the hydrophilic functional groups on the fiber surface were partially eliminated by modification, facilitating the degradation of different fiber components. Furthermore, the degree of fibrillation was improved, and more interfaces with asphalt components were formed, thus enhancing the high-temperature deformation resistance of asphalt binders, but slightly impairing its low-temperature performance. Among all modification methods, the ternary composite modification exerted important influences on fiber structure, oil absorption, and rheological properties of asphalt binders, significantly enhancing the performance of asphalt mixtures. Combined with surface modification methods, bagasse fibers would be promising reinforced pavement materials. Full article

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