The 2023 MDPI Annual Report has
been released!
 
25 pages, 2085 KiB  
Article
Size and Shape Selective Classification of Nanoparticles
by Cornelia Damm, Danny Long, Johannes Walter and Wolfgang Peukert
Powders 2024, 3(2), 255-279; https://doi.org/10.3390/powders3020016 - 17 May 2024
Abstract
As nanoparticle syntheses on a large scale usually yield products with broad size and shape distributions, the properties of nanoparticle-based products need to be tuned after synthesis by narrowing the size and shape distributions or via the removal of undesired fractions. The development [...] Read more.
As nanoparticle syntheses on a large scale usually yield products with broad size and shape distributions, the properties of nanoparticle-based products need to be tuned after synthesis by narrowing the size and shape distributions or via the removal of undesired fractions. The development of property-selective classification processes requires a universal framework for the quantitative evaluation of multi-dimensional particle fractionation processes. This framework must be applicable to any property and any particle classification process. We extended the well-known one-dimensional methodology commonly used for describing particle size distributions and fractionation processes to the multi-dimensional case to account for the higher complexity of the property distribution and separation functions. In particular, multi-dimensional lognormal distributions are introduced and applied to diameter and length distributions of gold nanorods. The fractionation of nanorods via centrifugation and by orthogonal centrifugal and electric forces is modeled. Moreover, we demonstrate that analytical ultracentrifugation with a multi-wavelength detector (MWL-AUC) is a fast and very accurate method for the measurement of two-dimensional particle size distributions in suspension. The MWL-AUC method is widely applicable to any class of nanoparticles with size-, shape- or composition-dependent optical properties. In addition, we obtained distributions of the lateral diameter and the number of layers of molybdenum disulfide nanosheets via stepwise centrifugation and spectroscopic evaluation of the size fractions. Full article
13 pages, 472 KiB  
Article
Academic Performance of Excellence: The Impact of Self-Regulated Learning and Academic Time Management Planning
by Abílio Afonso Lourenço and Maria Olímpia Paiva
Knowledge 2024, 4(2), 289-301; https://doi.org/10.3390/knowledge4020016 - 17 May 2024
Abstract
The Program for International Student Assessment highlights the persistent lack of commitment and motivation among students worldwide in their school activities, which are resulting in decreased proficiency levels in reading, mathematics, and science. The magnitude of this phenomenon, with its clear social implications, [...] Read more.
The Program for International Student Assessment highlights the persistent lack of commitment and motivation among students worldwide in their school activities, which are resulting in decreased proficiency levels in reading, mathematics, and science. The magnitude of this phenomenon, with its clear social implications, suggests that we are facing a concerning quest for immediate answers and results. This research focuses on the impact of the relationships between self-regulated learning processes and the planning of time management that is dedicated to school activities on student performance, specifically in the subjects of the Mother Tongue and Mathematics. The instruments used for analysis included the Inventory of Self-Regulated Learning Processes, the Inventory of Time Management Planning, a personal data sheet, and a school data sheet. The sample in this study consisted of 688 students from primary schools in northern Portugal. The results reveal that self-regulated learning has a positive influence on how students plan time management, both in the short and long term. Additionally, a positive and statistically significant relationship is observed between short-term and long-term time management planning and students’ academic performance. This study provides an in-depth perspective on the dynamics between these elements, shedding light on the crucial nuances that shape students’ academic journeys. Full article
12 pages, 37783 KiB  
Article
Microscopic Image Dataset with Segmentation and Detection Labels for Microplastic Analysis in Sewage: Enhancing Research and Environmental Monitoring
by Gwanghee Lee, Jaeheon Jung, Sangjun Moon, Jihyun Jung and Kyoungson Jhang
Microplastics 2024, 3(2), 264-275; https://doi.org/10.3390/microplastics3020016 - 17 May 2024
Abstract
We introduce a novel microscopic image dataset augmented with segmentation and detection labels specifically designed for microplastic analysis in sewage environments. Recognizing the increasing concern over microplastics—particles of synthetic polymers smaller than 5 mm—and their detrimental effects on marine ecosystems and human health, [...] Read more.
We introduce a novel microscopic image dataset augmented with segmentation and detection labels specifically designed for microplastic analysis in sewage environments. Recognizing the increasing concern over microplastics—particles of synthetic polymers smaller than 5 mm—and their detrimental effects on marine ecosystems and human health, our research focuses on enhancing detection and analytical methodologies through advanced computer vision and deep learning techniques. The dataset comprises high-resolution microscopic images of microplastics collected from sewage, meticulously labeled for both segmentation and detection tasks, aiming to facilitate accurate and efficient identification and quantification of microplastic pollution. In addition to dataset development, we present example deep learning models optimized for segmentation and detection of microplastics within complex sewage samples. The models demonstrate significant potential in automating the analysis of microplastic contamination, offering a scalable solution to environmental monitoring challenges. Furthermore, we ensure the accessibility and reproducibility 12 of our research by making the dataset and model codes publicly available, accompanied by detailed 13 documentation on GitHub and LabelBox. Full article
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14 pages, 387 KiB  
Article
Molecular Markers in Melanoma Progression: A Study on the Expression of miRNA Gene Subtypes in Tumoral vs. Benign Nevi
by Mihaela Prodan, Sergiu Costescu, Ahmed Elagez, Sorina Maria Denisa Laitin, Vlad Bloanca, Zorin Crainiceanu, Edward Seclaman, Ana-Olivia Toma, Roxana Manuela Fericean, George Puenea and Gabriel Veniamin Cozma
Curr. Oncol. 2024, 31(5), 2881-2894; https://doi.org/10.3390/curroncol31050220 - 17 May 2024
Abstract
This study investigates the differential expression of miRNA gene subtypes in tumoral versus benign nevi in individuals with melanoma, aiming to identify clinically significant correlations that could serve as reliable markers for assessing tumor stage and progression. Conducted between 2019 and 2022, this [...] Read more.
This study investigates the differential expression of miRNA gene subtypes in tumoral versus benign nevi in individuals with melanoma, aiming to identify clinically significant correlations that could serve as reliable markers for assessing tumor stage and progression. Conducted between 2019 and 2022, this descriptive, quantitative observational research analyzed 90 formalin-fixed paraffin-embedded (FFPE) samples from the Pius Brinzeu County Emergency Clinical Hospital, Timisoara, including 45 samples of advanced-stage melanoma and 45 samples of pigmented nevi. miRNA purification and analysis were performed using the miRNeasy Kit and the Human Cancer PathwayFinder miScript miRNA PCR Array, with statistical analysis (including logistic regression) to determine associations with cancer staging, such as high Breslow index risk, number of mitoses, and vascular invasion. After the analysis and comparison of 180 miRNA gene subtypes, we selected 10 of the most upregulated and 10 most downregulated genes. The results revealed that hsa-miR-133b, hsa-miR-335-5p, hsa-miR-200a-3p, and hsa-miR-885-5p were significantly upregulated in melanoma samples, with fold changes ranging from 1.09 to 1.12. Conversely, hsa-miR-451a and hsa-miR-29b-3p showed notable downregulation in melanoma, with fold changes of 0.90 and 0.92, respectively. Additionally, logistic regression analysis identified hsa-miR-29b-3p (OR = 2.51) and hsa-miR-200a-3p (OR = 2.10) as significantly associated with an increased risk of a high Breslow index, while hsa-miR-127-3p and hsa-miR-451a were associated with a reduced risk. Conclusively, this study underscores the significant alterations in miRNA expression in melanoma compared to benign nevi and highlights the potential of specific miRNAs as biomarkers for melanoma progression. The identification of miRNAs with significant associations to melanoma characteristics suggests their utility in developing non-invasive, cost-effective diagnostic tools and in guiding therapeutic decisions, potentially improving patient outcomes in melanoma management. Full article
(This article belongs to the Special Issue Epidemiology and Risk Factors of Skin Cancer)
31 pages, 2416 KiB  
Article
Investigating and Improving Pedestrian Safety in an Urban Environment of a Low- or Middle-Income Country: A Case Study of Yaoundé, Cameroon
by Steffel Ludivin Tezong Feudjio, Dimitri Tchaheu Tchaheu, Stephen Kome Fondzenyuy, Isaac Ndumbe Jackai II, Davide Shingo Usami and Luca Persia
Future Transp. 2024, 4(2), 548-578; https://doi.org/10.3390/futuretransp4020026 - 17 May 2024
Abstract
In Yaoundé, Cameroon, where walking dominates transport modes, pedestrian safety remains an issue as pedestrians account for a fair share of road traffic casualties, partly due to the lack of walking policies and pedestrian facilities safety data, hindering targeted intervention. This study used [...] Read more.
In Yaoundé, Cameroon, where walking dominates transport modes, pedestrian safety remains an issue as pedestrians account for a fair share of road traffic casualties, partly due to the lack of walking policies and pedestrian facilities safety data, hindering targeted intervention. This study used a pedestrian safety index (PSI) and the Global Walkability Index (GWI) to investigate 12 road segments frequented by diverse pedestrian groups. Indexes were graded from E—lowest to A—highest and analyzed using description and rank correlation. Main safety issues included lack of adequate and accessible sidewalks, bollards, pedestrian crossings, signage, shade, and street lighting. Only one segment (R7) achieved grade C, while the remainder scored D or E, indicating poor pedestrian safety conditions and an unpleasant walking experience. The correlation coefficient (0.69) between the PSI and GWI at a 99% significance level validated the safety assessment, providing confidence in the results. A seven-year (2024–2030) safety strategy is proposed to improve all roads to grade B. This strategy contains several interventions, including engineering improvement, which have been proven effective. This study offers evidence for city officials to improve pedestrian safety and informs walking policies and the implementation of upcoming projects. Future research should quantify the recommendations’ benefits and validate indexes with crash or conflict data. Full article
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19 pages, 3812 KiB  
Article
Evaluation of Tactile and Thermophysiological Comfort in Reusable Surgical Gowns Compared to Disposable Gowns
by Magdalena Georgievska, Abreha Bayrau Nigusse, Benny Malengier, Hasan Riaz Tahir, Charlotte Harding, Sufiyan Derbew Tiku and Lieva Van Langenhove
Textiles 2024, 4(2), 237-255; https://doi.org/10.3390/textiles4020014 - 17 May 2024
Abstract
Though the transition from disposable to reusable surgical gowns holds substantial promise, successful implementation faces challenges. This study investigated tactile and thermophysiological comfort in surgical reusable gowns, comparing them with their disposable counterparts. Parameters such as surface roughness, compression, heat flux, and material [...] Read more.
Though the transition from disposable to reusable surgical gowns holds substantial promise, successful implementation faces challenges. This study investigated tactile and thermophysiological comfort in surgical reusable gowns, comparing them with their disposable counterparts. Parameters such as surface roughness, compression, heat flux, and material rigidity were tested using a Fabric Touch Tester. Additionally, the water vapour permeability and static charge of the gowns were assessed. Thermophysiological comfort of the gowns was evaluated by measuring the temperature and relative humidity (RH) on test subjects during wear trials where they were engaged in an activity that mimics a surgeon’s performance. Skin temperature was monitored using iButton sensors and a thermal camera, and the impact on heart rate during the task was analysed. Following each test, participants provided subjective feedback through a questionnaire. The results indicated that reusable gowns boasted a smoother texture, translating to reduced friction on the skin and better heat transfer compared to the disposable fabrics, as indicated using FTT. They also exhibited higher water vapour permeability compared to their disposable counterparts. The wear trials revealed minimal differences in comfort between disposable and reusable gowns. While performing the activity, an increase in body temperature led to decreased RH, yet this rise did not adversely affect subject comfort, as validated using heart rate and questionnaire survey data. From a comfort point of view, switching from disposable to reusable gowns would not have drawbacks, meaning hospitals should be able to switch provided logistics and costs can be managed. Full article
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16 pages, 1997 KiB  
Article
Feasibility of Using Green Laser for Underwater Infrastructure Monitoring: Case Studies in South Florida
by Rahul Dev Raju, Sudhagar Nagarajan, Madasamy Arockiasamy and Stephen Castillo
Geomatics 2024, 4(2), 173-188; https://doi.org/10.3390/geomatics4020010 - 17 May 2024
Abstract
Scour around bridges present a severe threat to the stability of railroad and highway bridges. Scour needs to be monitored to prevent the bridges from becoming damaged. This research studies the feasibility of using green laser for monitoring the scour around candidate railroad [...] Read more.
Scour around bridges present a severe threat to the stability of railroad and highway bridges. Scour needs to be monitored to prevent the bridges from becoming damaged. This research studies the feasibility of using green laser for monitoring the scour around candidate railroad and highway bridges. The laboratory experiments that provided the basis for using green laser for underwater mapping are also discussed. The results of the laboratory and field experiments demonstrate the feasibility of using green laser for underwater infrastructure monitoring with limitations on the turbidity of water that affects the penetrability of the laser. This method can be used for scour monitoring around offshore structures in shallow water as well as corrosion monitoring of bridges. Full article
26 pages, 5983 KiB  
Article
Geoinformatics-Based Mapping of Environmental Sensitive Areas for Desertification over Satara and Sangli Districts of Maharashtra, India
by Chandra Shekhar Dwivedi, Dishant, Bikash Ranjan Parida, Arvind Chandra Pandey, Ravi Kumar and Navneet Kumar
GeoHazards 2024, 5(2), 415-440; https://doi.org/10.3390/geohazards5020022 - 17 May 2024
Abstract
Desertification processes in arid, semi-arid, and dry sub-humid conditions have been enhanced in recent decades. The geospatial database and associated satellite data can be effectively employed for regional planning to address desertification and land degradation. In this study, the Mediterranean Desertification and Land [...] Read more.
Desertification processes in arid, semi-arid, and dry sub-humid conditions have been enhanced in recent decades. The geospatial database and associated satellite data can be effectively employed for regional planning to address desertification and land degradation. In this study, the Mediterranean Desertification and Land Use (MEDALUS) model has been used to map environmentally sensitive areas due to desertification in the Satara and Sangli districts of Maharashtra, India. This was achieved by combining Landsat-8 multispectral data, Census data, soil data, and climatic variables like temperature, rainfall, and evapotranspiration. The algorithm of MEDALUS is the geometric mean of four indicators, namely soil quality index (SQI), climate quality index (CQI), vegetation quality index (VQI), and socio-economic quality index (SEQI). The findings indicated that the majority of the study area comes under the potential category of desertification (60.32%) followed by fragile (27.87%) and critical (11.81%). Areas with a high propensity for desertification were found over the low to very low climatic quality and moderate to high soil quality including lower socio-economic quality. The lower socio-economic quality is mainly due to high to very high population density (>100 people/km2), low to moderate illiteracy rate (<16%), and low to moderate work participation rate (<50%) that incentivize unsustainable land use practices. The study provides a valuable tool for understanding and managing natural resources. It offers a detailed analysis of the environmental sensitivity of the study area, taking into account various factors like land use, vegetation cover, slope, and soil erosion potential. The developed comprehensive map of the area helps in identifying the most sensitive regions and developing appropriate conservation strategies. The information obtained from the study can be utilized to develop and implement successful measures to prevent or alleviate desertification, which is crucial for sustaining the health of ecosystems and the welfare of local residents. Full article
12 pages, 443 KiB  
Article
Relationship between Maternal Socioeconomic Factors and Preterm Birth in Latvia
by Katrīne Kūkoja, Anita Villeruša and Irisa Zīle-Velika
Medicina 2024, 60(5), 826; https://doi.org/10.3390/medicina60050826 - 17 May 2024
Abstract
Background and Objectives: Worldwide, preterm birth (PTB) stands as the primary cause of mortality among children under 5 years old. Socioeconomic factors significantly impact pregnancy outcomes, influencing both maternal well-being and newborn health. Understanding and addressing these socioeconomic factors is essential for developing [...] Read more.
Background and Objectives: Worldwide, preterm birth (PTB) stands as the primary cause of mortality among children under 5 years old. Socioeconomic factors significantly impact pregnancy outcomes, influencing both maternal well-being and newborn health. Understanding and addressing these socioeconomic factors is essential for developing effective public health interventions and policies aimed at improving pregnancy outcomes. This study aims to analyse the relationship between socioeconomic factors (education level, marital status, place of residence and nationality) and PTB in Latvia, considering mother’s health habits, health status, and pregnancy process. Materials and Methods: A cross-sectional study was conducted using data from the Medical Birth Register (MBR) of Latvia about women with singleton pregnancies in 2022 (n = 15,431). Data analysis, involving crosstabs, chi-square tests, and multivariable binary logistic regression, was performed. Adjusted Odds ratios (aOR) with 95% confidence intervals (CI) were estimated. Results: Lower maternal education was statistically significantly associated with increased odds of PTB. Mothers with education levels below secondary education had over two times higher odds of PTB (aOR = 2.07, p < 0.001, CI 1.58–2.70) and those with secondary or vocational secondary education had one and a half times higher odds (aOR = 1.58, p < 0.001, CI 1.33–1.87) after adjusting for other risk factors. Study results also showed the cumulative effect of socioeconomic risk factors on PTB. Additionally, mothers facing two or three socioeconomic risk factors in Latvia exhibited one and a half times higher odds of PTB (aOR = 1.59, p = 0.021). Conclusions: The study highlights the cumulative impact of socioeconomic risk factors on PTB, with higher maternal education demonstrating the highest protective effect against it. This underscores the importance of education in promoting optimal foetal development. Since the influence of socioeconomic factors on PTB is not a widely studied issue in Latvia, further research is needed to improve understanding of this complex topic. Full article
(This article belongs to the Section Epidemiology & Public Health)
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7 pages, 321 KiB  
Brief Report
Food Insecurity Is Associated with a Higher Risk of Mortality among Colorectal Cancer Survivors
by Jagdish Khubchandani, Srikanta Banerjee, Rafael Gonzales-Lagos and Karen Kopera-Frye
Gastrointest. Disord. 2024, 6(2), 461-467; https://doi.org/10.3390/gidisord6020031 - 17 May 2024
Abstract
Purpose: Food insecurity and colorectal cancer (CRC) are widely prevalent problems in the U.S. However, the long-term effects of food insecurity among people living with CRC are not well explored (e.g., risk of mortality). Methods: Data from the U.S. National Health and Nutrition [...] Read more.
Purpose: Food insecurity and colorectal cancer (CRC) are widely prevalent problems in the U.S. However, the long-term effects of food insecurity among people living with CRC are not well explored (e.g., risk of mortality). Methods: Data from the U.S. National Health and Nutrition Examination Survey (years 1999–2010) were linked with mortality data from the National Death Index up to 31 December 2019. Results: A total of 30,752 adults comprised the analytic sample; 222 were living with CRC and more than a tenth were food-insecure (11.6%). In our adjusted analysis, individuals who were food insecure and had CRC were 4.13 times more likely to die of any cause and 9.57 times more likely to die of cardiovascular diseases (compared to those without CRC and food insecurity). Conclusions: Colorectal cancer is among the top cancers diagnosed in American adults and more than a tenth of adult Americans with CRC live with food insecurity. Given the higher risk of mortality with co-occurring CRC and food insecurity, collaborative healthcare models can help address food insecurity and other social needs of people with CRC, and surveillance measures for food insecurity should be widely implemented across health systems. Full article
(This article belongs to the Special Issue Feature Papers in Gastrointestinal Disorders in 2023-2024)
14 pages, 503 KiB  
Article
Researching Gender and Disasters of Natural Origin: Ethical Challenges
by Sandra Dema Moreno, María Teresa Alonso Moro and Virginia Cocina Díaz
Philosophies 2024, 9(3), 70; https://doi.org/10.3390/philosophies9030070 - 17 May 2024
Abstract
Ethical issues are very relevant in the field of women’s, gender and/or feminist studies. The aim of this article is to highlight the ethical challenges faced by the authors in their research process, with specific reference to two projects on gender and disasters [...] Read more.
Ethical issues are very relevant in the field of women’s, gender and/or feminist studies. The aim of this article is to highlight the ethical challenges faced by the authors in their research process, with specific reference to two projects on gender and disasters in which they have been involved. In general, we try to avoid sexist bias throughout the complete research process, from the definition of the objectives themselves to the methodology design, where we ensure diversity in the selection of participants in order to take into consideration the variety of voices present in society, especially those of women. Also, when developing our research, we take into account the power relationships involved, both between those who participate in the fieldwork and with the researchers themselves. To counteract the effects of such relations, we have considered people’s wellbeing and the humanization of the whole process. Finally, when it comes to the dissemination of the results and their transfer to society at large, we follow the same principles and actively integrate the people involved. Considering these issues benefits the research process and makes the resultant knowledge more ethical and socially useful, in addition to promoting more egalitarian gender relations. Full article
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11 pages, 3185 KiB  
Communication
Occurrence of Four Freshwater Stingrays (Chondrichthyes: Potamotrygoninae) in the Uatumã River Basin, Amazon Region: A Field Study
by Maria Fernanda S. Gomes, Paulo Henrique R. Aride, Maiko Willas S. Ribeiro, Cristiane C. Guimarães, Tiago C. Nóbrega, Rayana M. Paixão, Adriene Núzia A. Santos and Adriano Teixeira de Oliveira
Fishes 2024, 9(5), 186; https://doi.org/10.3390/fishes9050186 - 17 May 2024
Abstract
The Amazon region has the largest diversity of freshwater stingrays; however, there are still places where information about this group is scarce. The present work aims to record the occurrence of freshwater stingrays in the Uatumã river basin, Amazonas, Brazil. For this, collections [...] Read more.
The Amazon region has the largest diversity of freshwater stingrays; however, there are still places where information about this group is scarce. The present work aims to record the occurrence of freshwater stingrays in the Uatumã river basin, Amazonas, Brazil. For this, collections were carried out in three municipalities bathed by the Uatumã River, Presidente Figueiredo, Itapiranga, and São Sebastião do Uatumã. The rays were collected through fishing using a hand net and, after capture, were identified through the pattern of the dorsal and ventral color of the disc, the absence or presence of the labial groove, and the organization of the row of spines on the tail, and were also measured, weighed, and sexed. In total, 69 specimens of Potamotrygonines were captured, 59 of which were from the genus Potamotrygon and ten individuals from the genus Paratrygon. The present study provides preliminary information on the characteristics of freshwater stingray species that may occur in the Uatumã River. Furthermore, new research is necessary to improve the identification of specimens to determine the diversity and elucidate aspects related to the biology of elasmobranchs in the Uatumã River Basin, Brazil. Full article
(This article belongs to the Section Biology and Ecology)
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18 pages, 3424 KiB  
Article
Waiting to Be Discovered? Community Partnerships, the Facilitation of Diverse Memory, and Reflections on Academic Success and Failure
by Chris Kempshall, Catriona Pennell and Felicity Tattersall
Genealogy 2024, 8(2), 62; https://doi.org/10.3390/genealogy8020062 - 17 May 2024
Abstract
Community partnerships, based on ‘the collaborative turn’ in academic research, are an increasingly common framework through which ‘bottom-up’ histories, particularly of diverse and/or more marginalised communities, are being told. This article is about the ‘doing’ of this type of work. It focuses on [...] Read more.
Community partnerships, based on ‘the collaborative turn’ in academic research, are an increasingly common framework through which ‘bottom-up’ histories, particularly of diverse and/or more marginalised communities, are being told. This article is about the ‘doing’ of this type of work. It focuses on the question: what lessons can be made visible when attempted cooperation fails to deliver the outcomes initially hoped for? Firstly, this article outlines the events and activities undertaken by the authors in exploring the ways that ephemera and other objects can be used to understand and transmit the historical experiences of communities often on the periphery of mainstream war commemoration. It will discuss the ways in which connections with these communities were built, with the aim of undertaking several creative writing workshops, leading to a co-produced publication of the participants’ material. Secondly, as part of a broader acknowledgment of the possibility of failure and its benefits, it will explore why some of these creative workshop efforts failed to meet expectations and outline a series of recommendations for other historians and community-orientated projects to consider for future activities. Full article
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15 pages, 2524 KiB  
Article
Jasmonates Play an Important Role in Differential Accumulation of Key Oolong Tea Aromas in Two Tea Varieties (Camellia sinensis)
by Xin-Lei Li, Hui-Li Deng, Yu-Cheng Zheng, Xiang-Rui Kong, Qiu-Sheng Zhong, Xiao-Mei You, Rui-Yang Shan, Zheng-He Lin, Zhi-Hui Chen and Chang-Song Chen
Horticulturae 2024, 10(5), 520; https://doi.org/10.3390/horticulturae10050520 - 17 May 2024
Abstract
Aroma is an important factor in the measurement of the quality and market value of oolong tea. However, it is hard to develop an oolong tea with good aroma quality using unsuitable tea varieties. To explore the key factors of tea varieties in [...] Read more.
Aroma is an important factor in the measurement of the quality and market value of oolong tea. However, it is hard to develop an oolong tea with good aroma quality using unsuitable tea varieties. To explore the key factors of tea varieties in the formation of oolong tea aromas, the fresh leaves of the Chungui variety (CG, suitable for oolong tea, Camellia sinensis (L.) O. Kuntze) and the Fuyun No. 6 variety (F6, unsuitable for oolong tea, Camellia sinensis (L.) O. Kuntze) were harvested and treated by withering and mechanical stress in order. Then, aroma, transcriptome, and jasmonate (JA) contents, and weighted gene co-expression network analysis (WGCNA), of samples were investigated. The contents of characteristic oolong tea aromas, including indole, (E)-β-ocimene, (E)-nerolidol, α-farnesene, and jasmine lactone, were all accumulated in much higher quantities in the CG variety after withering and mechanical stress. Accordingly, the coding genes of aroma formation synthases TSB2, OCS, NES, AFS, and LOX1, and related genes in MVA, MEP, and ALA pathways, were all much more highly activated. These differential reactions are mainly caused by the higher accumulation of jasmonates, especially methyl jasmonate, a type of important plant signal chemical, in CG after mechanical stress. WGCNA analysis indicated 34 different transcription factors from different families are predicted to be involved in this jasmonate-responsive reaction. Full article
(This article belongs to the Section Medicinals, Herbs, and Specialty Crops)
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13 pages, 2067 KiB  
Article
The Effect of Battery Configuration on Dendritic Growth: A Magnetic Resonance Microscopy Study on Symmetric Lithium Cells
by Rok Peklar, Urša Mikac and Igor Serša
Batteries 2024, 10(5), 165; https://doi.org/10.3390/batteries10050165 - 17 May 2024
Abstract
The potential of metallic lithium to become the anode material for next-generation batteries is hampered by significant challenges, chief among which is dendrite growth during battery charging. These dendritic structures not only impair battery performance but also pose safety risks. Among the non-destructive [...] Read more.
The potential of metallic lithium to become the anode material for next-generation batteries is hampered by significant challenges, chief among which is dendrite growth during battery charging. These dendritic structures not only impair battery performance but also pose safety risks. Among the non-destructive analytical techniques in battery research, Magnetic Resonance Imaging (MRI) stands out as a promising tool. However, the direct imaging of lithium by 7Li MRI is limited by its low sensitivity and spatial resolution, making it a less effective way of imaging dendrite growth. Instead, a recently introduced indirect imaging approach which is based on 1H MRI of the electrolyte was used in this study. This method was used to sequentially 3D image and thus monitor the charging process of lithium metal symmetric cells in three different electrical circuits, namely those composed of a single cell, four cells in parallel, and four cells in series. The measured sequential images allowed for the measurement of dendrite growth in each cell using volumetric analysis. The growth results confirmed the theoretical prediction that the growth across cells is uneven in a parallel circuit, and even in a series circuit. The methods presented in this study can also be applied to analyze many other dendrite-related issues in batteries. Full article
(This article belongs to the Special Issue Towards a Smarter Battery Management System)
19 pages, 4074 KiB  
Article
MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-Transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Member Expression
by Estefanía Lozano-Velasco, Carlos Garcia-Padilla, Miguel Carmona-Garcia, Alba Gonzalez-Diaz, Angela Arequipa-Rendon, Amelia E. Aranega and Diego Franco
Non-Coding RNA 2024, 10(3), 32; https://doi.org/10.3390/ncrna10030032 - 17 May 2024
Abstract
Transcriptional regulation constitutes a key step in gene expression regulation. Myocyte enhancer factor 2C (MEF2C) is a transcription factor of the MADS box family involved in the early development of several cell types, including muscle cells. Over the last decade, a novel layer [...] Read more.
Transcriptional regulation constitutes a key step in gene expression regulation. Myocyte enhancer factor 2C (MEF2C) is a transcription factor of the MADS box family involved in the early development of several cell types, including muscle cells. Over the last decade, a novel layer of complexity modulating gene regulation has emerged as non-coding RNAs have been identified, impacting both transcriptional and post-transcriptional regulation. microRNAs represent the most studied and abundantly expressed subtype of small non-coding RNAs, and their functional roles have been widely documented. On the other hand, our knowledge of the transcriptional and post-transcriptional regulatory mechanisms that drive microRNA expression is still incipient. We recently demonstrated that MEF2C is able to transactivate the long, but not short, regulatory element upstream of the miR-23a-miR-27a-miR-24-2 transcriptional start site. However, MEF2C over-expression and silencing, respectively, displayed distinct effects on each of the miR-23a-miR-27a-miR-24-2 mature cluster members without affecting pri-miRNA expression levels, thus supporting additional MEF2C-driven regulatory mechanisms. Within this study, we demonstrated a complex post-transcriptional regulatory mechanism directed by MEF2C in the regulation of miR-23a-miR-27a-miR-24-2 cluster members, distinctly involving different domains of the MEF2C transcription factor and the physical interaction with pre-miRNAs and Ksrp, HnRNPa3 and Ddx17 transcripts. Full article
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17 pages, 6833 KiB  
Data Descriptor
Continuous Wave Measurements Collected in Intermediate Depth throughout the North Sea Storm Season during the RealDune/REFLEX Experiments
by Jantien Rutten, Marion Tissier, Paul van Wiechen, Xinyi Zhang, Sierd de Vries, Ad Reniers and Jan-Willem Mol
Data 2024, 9(5), 70; https://doi.org/10.3390/data9050070 - 17 May 2024
Abstract
High-resolution wave measurements at intermediate water depth are required to improve coastal impact modeling. Specifically, such data sets are desired to calibrate and validate models, and broaden the insight on the boundary conditions that force models. Here, we present a wave data set [...] Read more.
High-resolution wave measurements at intermediate water depth are required to improve coastal impact modeling. Specifically, such data sets are desired to calibrate and validate models, and broaden the insight on the boundary conditions that force models. Here, we present a wave data set collected in the North Sea at three stations in intermediate water depth (6–14 m) during the 2021/2022 storm season as part of the RealDune/REFLEX experiments. Continuous measurements of synchronized surface elevation, velocity and pressure were recorded at 2–4 Hz by Acoustic Doppler Profilers and an Acoustic Doppler Velocimeter for a 5-month duration. Time series were quality-controlled, directional-frequency energy spectra were calculated and common bulk parameters were derived. Measured wave conditions vary from calm to energetic with 0.1–5.0 m sea-swell wave height, 5–16 s mean wave period and W-NNW direction. Nine storms, i.e., wave height beyond 2.5 m for at least six hours, were recorded including the triple storms Dudley, Eunice and Franklin. This unique data set can be used to investigate wave transformation, wave nonlinearity and wave directionality for higher and lower frequencies (e.g., sea-swell and infragravity waves) to compare with theoretical and empirical descriptions. Furthermore, the data can serve to force, calibrate and validate models during storm conditions. Full article
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17 pages, 945 KiB  
Article
Investigation of the Role of Osteoporotic Vertebra Degeneration on the Stability of the Lumbar Spine: In Silico Modelling under Compressive Loading
by Olga Chabarova, Jelena Selivonec and Alicia Menendez Hurtado
Bioengineering 2024, 11(5), 507; https://doi.org/10.3390/bioengineering11050507 - 17 May 2024
Abstract
An evaluation of the impact of osteoporosis on loss of spinal stability, with or without intervertebral disc degeneration, using computational analysis is presented. The research also investigates the correlation between osteoporosis and intervertebral disc degeneration. Three-dimensional finite element models of human lumbar spine [...] Read more.
An evaluation of the impact of osteoporosis on loss of spinal stability, with or without intervertebral disc degeneration, using computational analysis is presented. The research also investigates the correlation between osteoporosis and intervertebral disc degeneration. Three-dimensional finite element models of human lumbar spine segments were used to assess the influence of osteoporosis on spinal stability. Five different models of age-related degeneration were created using various material properties for trabecular bone and intervertebral discs. Calculation results indicate that in a spine with osteoporosis, the deformation of the intervertebral discs can increase by more than 30% when compared to a healthy spine. Thus, intervertebral disc deformation depends not only on the degree of degeneration of the discs themselves, but their deformation is also influenced by the degree of osteoporosis of the vertebrae. Additionally, the load-bearing capacity of the spine can decrease by up to 30% with osteoporosis, regardless of the degree of intervertebral disc deformation. In conclusion, osteoporosis can contribute to intervertebral disc degeneration. Full article
(This article belongs to the Special Issue Recent Development in Spine Biomechanics)
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21 pages, 1386 KiB  
Review
Integration of Ultrasound in Image-Guided Adaptive Brachytherapy in Cancer of the Uterine Cervix
by Elena Manea, Elena Chitoran, Vlad Rotaru, Sinziana Ionescu, Dan Luca, Ciprian Cirimbei, Mihnea Alecu, Cristina Capsa, Bogdan Gafton, Iulian Prutianu, Dragos Serban and Laurentiu Simion
Bioengineering 2024, 11(5), 506; https://doi.org/10.3390/bioengineering11050506 - 17 May 2024
Abstract
Cervical cancer continues to be a public health concern, as it remains the second most common cancer despite screening programs. It is the third most common cause of cancer-related death for women, and the majority of cases happen in developing nations. The standard [...] Read more.
Cervical cancer continues to be a public health concern, as it remains the second most common cancer despite screening programs. It is the third most common cause of cancer-related death for women, and the majority of cases happen in developing nations. The standard treatment for locally advanced cervical cancer involves the use of external beam radiation therapy, along with concurrent chemotherapy, followed by an image-guided adaptive brachytherapy (IGABT) boost. The five-year relative survival rate for European women diagnosed with cervical cancer was 62% between 2000 and 2007. Updated cervical cancer treatment guidelines based on IGABT have been developed by the Gynecological working group, which is composed of the Group Européen de Curiethérapie–European Society for Therapeutic Radiology and Oncology. The therapeutic strategy makes use of three-dimensional imaging, which can be tailored to the target volume and at-risk organs through the use of computed tomography or magnetic resonance imaging. Under anaesthesia, the brachytherapy implantation is carried out. Ultrasonography is utilised to assess the depth of the uterine cavity and to facilitate the dilation of the uterine canal during the application insertion. In this study, we examine data from the international literature regarding the application of ultrasound in cervical cancer brachytherapy. Full article
(This article belongs to the Special Issue Image-Guided Radiation Therapy for Cancer)
24 pages, 7165 KiB  
Article
Subtractive Proteomics and Reverse-Vaccinology Approaches for Novel Drug Target Identification and Chimeric Vaccine Development against Bartonella henselae Strain Houston-1
by Sudais Rahman, Chien-Chun Chiou, Shabir Ahmad, Zia Ul Islam, Tetsuya Tanaka, Abdulaziz Alouffi, Chien-Chin Chen, Mashal M. Almutairi and Abid Ali
Bioengineering 2024, 11(5), 505; https://doi.org/10.3390/bioengineering11050505 - 17 May 2024
Abstract
Bartonella henselae is a Gram-negative bacterium causing a variety of clinical symptoms, ranging from cat-scratch disease to severe systemic infections, and it is primarily transmitted by infected fleas. Its status as an emerging zoonotic pathogen and its capacity to persist within host erythrocytes [...] Read more.
Bartonella henselae is a Gram-negative bacterium causing a variety of clinical symptoms, ranging from cat-scratch disease to severe systemic infections, and it is primarily transmitted by infected fleas. Its status as an emerging zoonotic pathogen and its capacity to persist within host erythrocytes and endothelial cells emphasize its clinical significance. Despite progress in understanding its pathogenesis, limited knowledge exists about the virulence factors and regulatory mechanisms specific to the B. henselae strain Houston-1. Exploring these aspects is crucial for targeted therapeutic strategies against this versatile pathogen. Using reverse-vaccinology-based subtractive proteomics, this research aimed to identify the most antigenic proteins for formulating a multi-epitope vaccine against the B. henselae strain Houston-1. One crucial virulent and antigenic protein, the PAS domain-containing sensor histidine kinase protein, was identified. Subsequently, the identification of B-cell and T-cell epitopes for the specified protein was carried out and the evaluated epitopes were checked for their antigenicity, allergenicity, solubility, MHC binding capability, and toxicity. The filtered epitopes were merged using linkers and an adjuvant to create a multi-epitope vaccine construct. The structure was then refined, with 92.3% of amino acids falling within the allowed regions. Docking of the human receptor (TLR4) with the vaccine construct was performed and demonstrated a binding energy of −1047.2 Kcal/mol with more interactions. Molecular dynamic simulations confirmed the stability of this docked complex, emphasizing the conformation and interactions between the molecules. Further experimental validation is necessary to evaluate its effectiveness against B. henselae. Full article
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13 pages, 1241 KiB  
Article
Discrimination of New and Aged Seeds Based on On-Line Near-Infrared Spectroscopy Technology Combined with Machine Learning
by Yanqiu Zhu, Shuxiang Fan, Min Zuo, Baohua Zhang, Qingzhen Zhu and Jianlei Kong
Foods 2024, 13(10), 1570; https://doi.org/10.3390/foods13101570 - 17 May 2024
Abstract
The harvest year of maize seeds has a significant impact on seed vitality and maize yield. Therefore, it is vital to identify new seeds. In this study, an on-line near-infrared (NIR) spectra collection device (899–1715 nm) was designed and employed for distinguishing maize [...] Read more.
The harvest year of maize seeds has a significant impact on seed vitality and maize yield. Therefore, it is vital to identify new seeds. In this study, an on-line near-infrared (NIR) spectra collection device (899–1715 nm) was designed and employed for distinguishing maize seeds harvested in different years. Compared with least squares support vector machine (LS-SVM), k-nearest neighbor (KNN), and extreme learning machine (ELM), the partial least squares discriminant analysis (PLS-DA) model has the optimal recognition performance for maize seed harvest years. Six different preprocessing methods, including Savitzky–Golay smoothing (SGS), standard normal variate transformation (SNV), multiplicative scatter correction (MSC), Savitzky–Golay 1 derivative (SG-D1), Savitzky–Golay 2 derivative (SG-D2), and normalization (Norm), were used to improve the quality of the spectra. The Monte Carlo cross-validation uninformative variable elimination (MC-UVE), competitive adaptive reweighted sampling (CARS), bootstrapping soft shrinkage (BOSS), successive projections algorithm (SPA), and their combinations were used to obtain effective wavelengths and decrease spectral dimensionality. The MC-UVE-BOSS-PLS-DA model achieved the classification with an accuracy of 88.75% using 93 features based on Norm preprocessed spectral data. This study showed that the self-designed NIR collection system could be used to identify the harvested years of maize seed. Full article
(This article belongs to the Special Issue Applications of Artificial Intelligence in Food Industry)
13 pages, 1278 KiB  
Article
Ultrasound-Assisted Nanoemulsion Loaded with Optimized Antibacterial Essential Oil Blend: A New Approach against Escherichia coli, Staphylococcus aureus, and Salmonella Enteritidis in Trout (Oncorhynchus mykiss) Fillets
by Luiz Torres Neto, Maria Lucia Guerra Monteiro, Bruno Dutra da Silva, Maxsueli Aparecida Moura Machado, Yhan da Silva Mutz and Carlos Adam Conte-Junior
Foods 2024, 13(10), 1569; https://doi.org/10.3390/foods13101569 - 17 May 2024
Abstract
This study aimed to obtain and characterize an oil-in-water nanoemulsion (NE) loaded with an in vitro optimized bactericidal essential oil blend of 50% oregano, 40% thyme, and 10% lemongrass and to evaluate its potential at three different concentrations (0.5%, 1%, and 2%) in [...] Read more.
This study aimed to obtain and characterize an oil-in-water nanoemulsion (NE) loaded with an in vitro optimized bactericidal essential oil blend of 50% oregano, 40% thyme, and 10% lemongrass and to evaluate its potential at three different concentrations (0.5%, 1%, and 2%) in the inactivation of Escherichia coli, Staphylococcus aureus, and Salmonella enterica serotype Enteritidis inoculated in rainbow trout fillets stored at 4 °C for 9 days. Regarding the NE, the nanometric size (<100 nm) with low polydispersion (0.17 ± 0.02) was successfully obtained through ultrasound at 2.09 W/cm2. Considering the three concentrations used, S. Enteritidis was the most susceptible. On the other hand, comparing the concentrations used, the NE at 2% showed better activity, reducing S. Enteritidis, E. coli, and S. aureus by 0.33, 0.20, and 0.73 log CFU/g, respectively, in the trout fillets. Thus, this data indicates that this is a promising eco-friendly alternative to produce safe fish for consumption and reduce public health risks. Full article
(This article belongs to the Section Food Quality and Safety)
15 pages, 7114 KiB  
Article
The Application of Rotary Twist Collecting Actuator Systems for Camellia oleifera Flower Bud Collection
by Qing Zhao, Lijun Li and Zhifeng Yang
Processes 2024, 12(5), 1023; https://doi.org/10.3390/pr12051023 - 17 May 2024
Abstract
Pollen transmission of Camellia oleifera is affected by climate and environment, and the quality of natural pollination is not high, which seriously affects the yield of Camellia oleifera. Artificial pollination is an effective way to solve the low fruit setting rate of [...] Read more.
Pollen transmission of Camellia oleifera is affected by climate and environment, and the quality of natural pollination is not high, which seriously affects the yield of Camellia oleifera. Artificial pollination is an effective way to solve the low fruit setting rate of natural pollination, but the problem to be solved urgently in artificial pollination is the collection of a large amount of pollen. At present, there is no mechanized equipment for Camellia oleifera flower bud collection, so developing an efficient pollen collection device has become a key problem that restricts the high-quality development of Camellia oleifera. In this paper, on the basis of measuring the tensile force, the shearing force, and the torsional moment required to remove Camellia Oleifera flower bud from the branch, which are 8.968 N, 13.94 N, and 0.0178 N·m, respectively, three types of Camellia oleifera flower bud collecting actuators were designed. According to the results of parameter design, feasibility analysis, and dynamic simulation, the power parameters of three types of Camellia oleifera flower bud collecting actuators were obtained. The experiment of collecting Camellia oleifera flower bud was designed, and the collection time, collection rate, and bud breakage rate of the three collecting actuators were compared. The experimental results show that the collection time of the rotary twist-type collecting actuator was 1.57 s, the collection rate was 91%, and the breakage rate was 4.9%, which can realize the efficient and low-loss collection of Camellia oleifera flowers bud, providing a theoretical basis for subsequent research on a Camellia oleifera flower bud collection robot. Full article
(This article belongs to the Section Manufacturing Processes and Systems)
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