Laurel wilt, a fungal disease (Harringtonia lauricola T.C. Harr., Fraedrich and Aghayeva) spread by the Asian redbay ambrosia beetle [Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae)], presents an imminent threat to North American members of the Lauraceae family, having caused extensive mortality in several species, especially redbay (Persea borbonia L. Spreng). Varying levels of disease resistance have been recorded in redbay under controlled conditions. To investigate if previously monitored putatively resistant field redbays have influenced the regeneration and survival of conspecifics within the surrounding 0.08 ha, a survey was conducted in 2018-19 and compared to similar data collected years prior (2008-09, 2013) along the coasts of Florida, Georgia, and South Carolina, United States. Plots were originally established at six disease-infested study sites around large redbay (>7.5-cm diameter at breast height (1.37 m) (DBH)) that had survived the initial laurel wilt disease epidemic that began in approximately 2007. In 2018-19, a subset of 61 plots within 16 m of the original “survivor” redbay were recorded and compared to previous surveys. Among the original redbay selected for resistance, 22 of 61 (36%) survivors across all sites were alive in 2018 with survival rates varying from 0 to 70% between survey periods (average mortality 3.6%/year). Trees that died in years since 2008-09 had their plots reclassified as susceptible or “suscepts”. Changes in mean quadratic diameter at 1.37 m of redbay plots near survivors were significantly greater than those near suscepts, and in 2018-19, the average diameter of redbay near survivors was 7.62 cm vs. 4.90 cm for suscepts. The diameter distribution of dead and live redbay in the whole population showed a decrease in live individuals surviving past 8 cm DBH in 2018–2019, but 20 of 22 survivor candidate trees were larger than 8 cm DBH. Regeneration was occurring both clonally and sexually and tended to be greater near suscepts, but midstory resprouts per hectare and understory seedlings interacted significantly with the site and the latter differed between sites. These findings indicate that redbay is regenerating in these ecosystems, and disease resistance may allow for increased average tree size for some individuals, but an upper size threshold of around 8 cm DBH may still exist for much of the population. In addition, the importance of site variables in regeneration was apparent, making either local genetic or environmental effects an important topic for future research. Continuing to monitor these survivors while locating new candidates for disease screenings and breeding, preventing the introductions of new strains of H. lauricola, shedding light on the nature of resistance and its heritability, and initiating outplanting trials with resistant germplasm are instrumental steps in bringing redbay back to prominence in its historical range. Full article

The present study employs X-ray photoelectron spectroscopy (XPS) to analyze plastic samples subjected to degradation processes with the aim to gain insight on the relevant chemical processes and disclose fragmentation mechanisms. Two model plastics, namely polystyrene (PS) and polyethylene (PE), are selected and analyzed before and after artificial UV radiation-triggered weathering, under simulated environmental hydrodynamic conditions, in fresh and marine water for different time intervals. The object of the study is to identify and quantify chemical groups possibly evidencing the occurrence of hydrolysis and oxidation reactions, which are the basis of degradation processes in the environment, determining macroplastic fragmentation. Artificially weathered plastic samples are analyzed also by Raman and FT-IR spectroscopy. Changes in surface chemistry with weathering are revealed by XPS, involving the increase in chemical moieties (hydroxyl, carbonyl, and carboxyl functionalities) which can be correlated with the degradation processes responsible for macroplastic fragmentation. On the other hand, the absence of significant modifications upon plastics weathering evidenced by Raman and FT-IR spectroscopy confirms the importance of investigating plastics surface, which represents the very first part of the materials exposed to degradation agents, thus revealing the power of XPS studies for this purpose. The XPS data on experimentally weathered particles are compared with ones obtained on microplastics collected from real marine environment for investigating the occurring degradation processes. Full article

This study investigates the impact of vapour-phase precursor flow rates—specifically those of trimethylaluminum (TMA) and deionized water (H₂O)—on the deposition of aluminum oxide (Al₂O₃) thin films through atomic layer deposition (ALD). It explores how these flow rates influence film growth kinetics and surface reactions, which are critical components of the ALD process. The research combines experimental techniques with a zero-dimensional theoretical model, designed specifically to simulate the deposition dynamics. This model integrates factors such as surface reactions and gas partial pressures within the ALD chamber. Experimentally, Al₂O₃ films were deposited at varied TMA and H₂O flow rates, with system conductance guiding these rates across different temperature settings. Film properties were rigorously assessed using optical reflectance methods and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. The experimental findings revealed a pronounced correlation between precursor flow rates and film growth. Specifically, at 150 °C, film thickness reached saturation at a TMA flow rate of 60 sccm, while at 200 °C, thickness peaked and then declined with increasing TMA flow above this rate. Notably, higher temperatures generally resulted in thinner films due to increased desorption rates, whereas higher water flow rates consistently produced thicker films, emphasizing the critical role of water vapour in facilitating surface reactions. This integrative approach not only deepens the understanding of deposition mechanics, particularly highlighting how variations in precursor flow rates distinctly affect the process, but also significantly advances operational parameters for ALD. These insights are invaluable for enhancing the application of ALD technologies across diverse sectors, including microelectronics, photovoltaics, and biomedical coatings, effectively bridging the gap between theoretical predictions and empirical results. Full article

Within the realm of organic synthesis, photocatalysis has blossomed since the beginning of the last decade. A plethora of classical reactivities, such as selective oxidation of alcohol and amines, redox radical formation of reactive species in situ, and indirect activation of an organic substrate for cycloaddition by EnT, have been revised in a milder and more sustainable fashion via photocatalysis. However, even though the spark of creativity leads scientists to explore new reactions and reactivities, the urgency of replacing the toxic and critical metals that are involved as catalysts has encouraged chemists to find alternatives in the branch of science called organocatalysis. Unfortunately, replacing metal catalysts with organic analogues can be too expensive sometimes; however, this drawback can be solved by the reutilization of the catalyst if it is heterogeneous. The aim of this review is to present the recent works in the field of heterogeneous photocatalysis, applied to organic synthesis, enabled by continuous flow. In detail, among the heterogeneous catalysts, g-CN, polymeric photoactive materials, and supported molecular catalysts have been discussed within their specific sections, rather than focusing on the types of reactions. Full article

Non-healing claw horn disorders are a serious problem in dairy herds because of the long duration of the disorder and the chronic pain derived from it, seriously affecting animal welfare and causing decreased production and premature culling from the herd. In a clinical trial, 40 cows in 13 herds (12 dairy herds and 1 herd with cow–calf operations) with toe necrosis (TN, 27x) or a non-healing white line disorder (NHWLD, 13x) were treated topically with an unguent-containing zinc sulphate and acetyl acid in combination with a parenteral injection of tilmycosin. An evaluation was conducted 3 months after treatment using locomotion scoring (LS), a clinical observation of the lesion, and the lactation value (the lactation value is the Net Profit of the individual animal divided by the average Net Profit of the entire herd. The mean is 100, so >100 is related to better production (combination of kg milk, %fat, and protein)) (LV) before and after treatment. The mean LS improved significantly from 4.0 (SD: 0.2) before treatment to 1.2 (SD: 0.4) 3 months after treatment (p < 0.001). The clinical presentation showed that all of the cows were cured from horn shoe infection (both TN and NHWLD). The LVs of the treated cows increased significantly from 111.2 (SD: 12.2) to 116.8 (SD: 15.1; p = 0.003). Full article

Metal halide perovskite semiconductors have emerged as promising materials for various optoelectronic applications due to their unique crystal structure and outstanding properties. Among different forms, perovskite nanowires (NWs) offer distinct advantages, including a high aspect ratio, superior crystallinity, excellent light absorption, and carrier transport properties, as well as unique anisotropic luminescence properties. Understanding the formation mechanism and structure–property relationship of perovskite NWs is crucial for exploring their potential in optoelectronic devices. In this study, we successfully synthesized all-inorganic halide perovskite NWs with high aspect ratios and an orthorhombic crystal phase using the hot-injection method with controlled reaction conditions and surface ligands. These NWs exhibit excellent optical and electrical properties. Moreover, precise control over the halogen composition through a simple anion exchange process enables the tuning of the bandgap, leading to fluorescence emission, covering a wide range of colors across the visible spectrum. Consequently, these perovskite NWs hold great potential for efficient energy conversion and catalytic applications in photoelectrocatalysis. Full article

It is demonstrated that the application of piezoelectric sensors to metallic bars and strands can enable determining the status of the integrity of these elements through the spectrum of their acoustic excitations. The voltage output of the piezo, secured to metal bars or strands, is fed to the input of a Fast Fourier Transform analyzer, which allows displaying the spectrum of the excitations from which information on the length, overall quality of the metal, and the presence of defects can be obtained. We show that the analysis, performed on several materials and strands of different lengths, could be useful for cases in which visible inspection and/or direct access to the entire body of the metallic elements is not possible. Applications of our study for testing metallic structures embedded in concrete foundations are reported for construction sites. Full article

Modern medicine has allowed for many advances in neurological and neurodegenerative disease (ND). However, the number of patients suffering from brain diseases is ever increasing and the treatment of brain diseases remains an issue, as drug efficacy is dramatically reduced due to the existence of the unique vascular structure, namely the blood–brain barrier (BBB). Several approaches to enhance drug delivery to the brain have been investigated but many have proven to be unsuccessful due to limited transport or damage induced in the BBB. Alternative approaches to enhance molecular delivery to the brain have been revealed in recent studies through the existence of molecular delivery pathways that regulate the passage of peripheral molecules. In this review, we present recent advancements of the basic research for these delivery pathways as well as examples of promising ventures to overcome the molecular hurdles that will enhance therapeutic interventions in the brain and potentially save the lives of millions of patients. Full article

Bacillus thuringiensis (Bt) secretes the nutritional insecticidal protein Vip3Aa11, which exhibits high toxicity against the fall armyworm (Spodoptera frugiperda). The Bt HD270 extracellular polysaccharide (EPS) enhances the toxicity of Vip3Aa11 protoxin against S. frugiperda by enhancing the attachment of brush border membrane vesicles (BBMVs). However, how EPS-HD270 interacts with Vip3Aa11 protoxin in vivo and the effect of EPS-HD270 on the toxicity of activated Vip3Aa11 toxin are not yet clear. Our results indicated that there is an interaction between mannose, a monosaccharide that composes EPS-HD270, and Vip3Aa11 protoxin, with a dissociation constant of Kd = 16.75 ± 0.95 mmol/L. When EPS-HD270 and Vip3Aa11 protoxin were simultaneously fed to third-instar larvae, laser confocal microscopy observations revealed the co-localization of the two compounds near the midgut wall, which aggravated the damage to BBMVs. EPS-HD270 did not have a synergistic insecticidal effect on the activated Vip3Aa11 protein against S. frugiperda. The activated Vip3Aa11 toxin demonstrated a significantly reduced binding capacity (548.73 ± 82.87 nmol/L) towards EPS-HD270 in comparison to the protoxin (34.96 ± 9.00 nmol/L). Furthermore, this activation diminished the affinity of EPS-HD270 for BBMVs. This study provides important evidence for further elucidating the synergistic insecticidal mechanism between extracellular polysaccharides and Vip3Aa11 protein both in vivo and in vitro. Full article

(1) Background: This scoping review aims to explore the literature on feedback for pharmacy students during experiential learning, with a focus on identifying the modes of delivery of feedback and the perceived impact of feedback on student learning outcomes. (2) Methods: The scoping review was conducted in accordance with the Joanna Briggs Institute (JBI) methodology and reported following the Preferred Reporting Items for Systematic Reviews Extension for Scoping Reviews (PRISMA-ScR) guidelines. PubMed, Web of Science, Embase, EBSCO, ERIC, and ProQuest Central were searched electronically from their inception until the end of February 2023 using a combination of keywords and MeSH terms related to feedback, pharmacy education, and student learning outcomes. Data were synthesized narratively. (3) Results: This review included 13 studies published between 2008 and 2022. Almost half of the included studies were conducted in the USA (n = 6, 46%) and reported the perspective of undergraduate pharmacy students (n = 6, 46%). Verbal feedback was the most common mode of feedback delivery (n = 6, 46%). The enablers of effective feedback included timely feedback (n = 6, 46%), feedback provided in a goal-oriented and objective manner (n = 5, 40%), and student-specific feedback (n = 4, 30%). On the other hand, the most common impediments to feedback efficacy were providing extremely positive feedback and lack of constructive criticism. (4) Conclusions: Our findings highlight the importance of feedback model implementation in pharmacy education and preceptor training programs to ensure effective and quality feedback to pharmacy students. Full article

Background: Non-steroidal anti-inflammatory drugs (NSAIDs) exacerbated respiratory disease (N-ERD) is associated with chronic rhinosinusitis with nasal polyps (CRSwNP), asthma, and NSAID hypersensitivity. An overproduction of leukotrienes characterizes the pathomechanism of the disease. N-ERD patients often report breathing difficulties after consuming alcohol. These symptoms have been observed in patients receiving either aspirin therapy after desensitization (ATAD), therapy with the biologics dupilumab (anti-IL-4Ra antibody) and omalizumab (anti-IgE antibody), or intranasal corticosteroid treatment (INCS). Methods: This retrospective, real-world study assessed the severity of alcohol-related and non-alcohol-related respiratory symptoms in CRSwNP/N-ERD patients 3–6 months after ATAD, biologic (dupilumab or omalizumab), or INCS therapy. A total of 171 patients (98 women and 73 men) were enrolled in the study. All groups received standard INCS therapy. Sixty-three patients were treated with ATAD; 48 received biologics (dupilumab n = 31; omalizumab n = 17); and 60 received INCS only and served as a control group. Alcohol-dependent symptoms and typical CRS symptoms (alcohol-independent) were quantified using visual analog scales (VAS). Results: ATAD and biological therapy significantly reduced VAS scores for alcohol dependence and CRS symptoms. In the control group receiving INCS, only non-alcohol dependent CRS symptoms improved significantly (p < 0.05). The most significant differences in pre/post scores were observed in patients receiving dupilumab, with the most significant improvement in alcohol-dependent and CRS symptoms (dupilumab > omalizumab > ATAD). Conclusions: This real-world study shows that alcohol-related respiratory symptoms are a relevant parameter in CRSwNP/N-ERD patients. Patients benefit more from biologic therapy than from ATAD in terms of their alcohol-related symptoms and other CRS symptoms. Future studies should include placebo-controlled oral alcohol challenge. Full article

Imbibing watermelon seeds in 1 mM sodium tetraborate (Na₂B₄O₇) for 24 h systemically protected plants against foliar infection by Stagonosporopsis cucurbitacearum in detached leaves and under greenhouse conditions. The treatment resulted in both a reduction in the overall percentage of leaf infection as well as in the size of lesions. Studies of the mechanisms by which Na₂B₄O₇ protected watermelon showed that there was no direct effect on the S. cucurbitacearum mycelium growth in vitro. On the other hand, plants raised from seeds primed with Na₂B₄O₇ showed a higher frequency of fluorescent epidermal cells compared to the plants treated with water. This indicates that a higher number of cells expressed the hypersensitive response after Na₂B₄O₇ priming. In addition, there was an increase in peroxidase activity and an enhanced accumulation of a 45 kDa acidic peroxidase isoform during the early stages of infection in plants treated with Na₂B₄O₇ compared to plants treated with water and this was positively correlated to the reduction of leaf infection caused by the pathogen. These results indicate that Na₂B₄O₇ is able to induce systemic resistance in watermelon against S. cucurbitacearum by activating the hypersensitive reaction at penetration sites, increasing peroxidase activity and altering the peroxidase isozyme profile. Although each individual response may only have had a minor effect, their combined effects had a reducing effect on the disease. Full article

Acute kidney injury (AKI) is common following liver transplantation and is associated with liver ischeamia reperfusion (IR) injury. The purpose of this study was to use a mouse model of liver IR injury and AKI to study the role of Neutrophil Gelatinase Associated Lipocalin (NGAL), a biomarker of AKI, in liver IR injury and AKI. We demonstrate an adapted, reproducible model of liver IR injury and AKI in which remote ischemic preconditioning (RIPC) by repeated episodes of hindleg ischemia prior to liver IR reduced the severity of the IR injury. In this model, serum NGAL at 2 h post reperfusion correlated with AKI development early following IR injury. This early rise in serum NGAL was associated with hepatic but not renal upregulation of NGAL mRNA, suggesting NGAL production in the liver but not the kidney in the early phase post liver IR injury. Full article

Antimicrobial stewardship involves a delicate balance between the risk of undertreating individuals and the potential societal burden of overprescribing antimicrobials. This balance is especially crucial in neonatal care. In this observational study, the usefulness of biomarkers of infectious diseases (calprotectin, procalcitonin (PCT), C-reactive protein (CRP), and white blood cells (WBCs) were evaluated in 141 febrile infants aged 28–90 days presenting to an emergency department. Since our focus was on the usefulness of serum calprotectin, this biomarker was not part of clinical decision-making. A significant difference was observed in the levels of all biomarkers, related to final discharge diagnosis and disposition status. The difference in levels related to antibiotic prescription was significant for all biomarkers but WBCs. The performance of calprotectin in the detection of bacterial infections (AUC (95% CI): 0.804 (0.691, 0.916)) was comparable to the performance of both PCT (0.901 (0.823, 0.980)) and CRP (0.859 (0.764, 0.953)) and superior to the WBC count (0.684 (0.544, 0.823)). Procalcitonin and CRP demonstrated a statistically significantly higher specificity relative to calprotectin. In this cohort, antibiotic use did not always correlate to a definite diagnosis of confirmed bacterial infection. The sample size was limited due to associated challenges with recruiting febrile infants. Hence, there is a need for adequate diagnostic tools to help discriminate between various kinds of infections. This study suggests serum calprotectin, procalcitonin, and CRP may serve as valuable biomarkers to differentiate between types of infection, in addition to clinical input and decision-making. Full article

This article presents a methodology for modeling the impact of both internal and external environmental carbon emission factors on the resulting indicators of an international company. This research uses picture fuzzy rough sets to model the impact of factors on the resulting indicators as a research method. The proposed model is based on a dataset that includes the company’s profit, revenue, valuation, share price, and market share from 2012 through 2022. This empirical period is optimal for such a type of modeling. An approach of picture fuzzy rough sets based on the time series of endogenous and exogenous variables can provide an opportunity to analyze and consider the consequences of feedback changes in the systems of which they are a part. The article proposes a valuable framework for understanding the complex relationship between carbon emissions, economic factors, and the performance of international companies. The researchers of this study recommend a discussion that attempts to gain a deeper understanding of the challenges and opportunities that lie ahead for international companies in the context of climate change and technological innovation. Full article

Recombinant adeno-associated viral (AAV) vectors have emerged as prominent gene delivery vehicles for gene therapy. AAV capsid proteins determine tissue specificity and immunogenicity and play important roles in receptor binding, the escape of the virus from the endosome, and the transport of the viral DNA to the nuclei of target cells. Therefore, the comprehensive characterization of AAV capsid proteins is necessary for a better understanding of the vector assembly, stability, and transduction efficiency of AAV gene therapies. Glycosylation is one of the most common post-translational modifications (PTMs) and may affect the tissue tropism of AAV gene therapy. However, there are few studies on the characterization of the N- and O-glycosylation of AAV capsid proteins. In this study, we identified the N- and O-glycosylation sites and forms of AAV9 capsid proteins generated from HEK293 cells using liquid chromatography–tandem mass spectrometry (LC-MS)-based glycopeptide mapping and identified free N-glycans released from AAV9 capsid proteins by PNGase F using hydrophilic interaction (HILIC) LC-MS and HILIC LC-fluorescence detection (FLD) methods. This study demonstrates that AAV9 capsids are sprinkled with sugars, including N- and O-glycans, albeit at low levels. It may provide valuable information for a better understanding of AAV capsids in supporting AAV-based gene therapy development. Full article

Adequate maternal nutrient intake of vitamin B12 is critical to fetal brain development and subsequent neurodevelopmental outcomes. We conducted a scoping review to map the current state of knowledge from human epidemiological studies on the associations between maternal vitamin B12 during pregnancy and children’s brain, cognitive, language, and motor development to identify gaps in the literature and suggest directions for future research. PubMed and OVID MEDLINE were searched. Search terms were vitamin B12, prenatal or maternal, neurodevelopment or cognitive development or brain. Animal studies were excluded. In total, 148 publications were identified, of which 19 met our inclusion criteria: (1) maternal vitamin B12 assessed via a measure of status, dietary intake, supplementation, or deficiency; and (2) an outcome related to brain development or cognitive, language, or motor development in children less than 18 years of age was assessed. This scoping review suggests that evidence supporting a relationship between maternal vitamin B12 during pregnancy and children’s neurodevelopmental outcomes is inconclusive. Further longitudinal research is needed to clarify the effects of maternal vitamin B12 supplementation, status, and intake on children’s brain development and neurodevelopmental outcomes. Full article

This research investigates the performance of small concrete beams that are reinforced with glass fiber-reinforced polymer (GFRP) bars, basalt fiber-reinforced polymer (BFRP) bars, and traditional steel bars. It addresses the limitations of traditional steel reinforcement, emphasizing the need for alternative strategies. Fiber-reinforced polymer (FRP) materials, including GFRP and BFRP, are examined for their mechanical characteristics compared to steel. The experimental program focuses on ultimate load-bearing capacity, deflection, deformation at different load levels, and failure modes. The concrete specimens, prepared according to Eurocode, consist of six small concrete beams measuring 80 × 120 × 1100 mm with varied reinforcements. The study reveals that GFRP-reinforced beams outperform BFRP and steel reinforcements in ultimate load-bearing capacity, showcasing enhanced structural performance. The GFRP-reinforced beams exhibit capacity and resilience characteristics surpassing those of both BFRP and steel, whereas the deflection observed was higher on both fiber-reinforced beams than on the steel-reinforced beams. The examination of failure modes reveals that the concrete beams that were reinforced with FRP bars showed a bending property before failure, while those reinforced with steel broke easily without bending much. This comprehensive research contributes to advancing our understanding of FRP materials’ application in concrete structures, paving the way for further optimization and overcoming limitations in reinforcement materials. Full article

MEK inhibitors (MEKi) represent innovative and promising treatments for managing manifestations of neurofibromatosis type 1 (NF1). To mitigate potential ophthalmic side effects, such as MEKi-associated retinopathy (MEKAR), patients undergoing MEKi therapy routinely receive ophthalmology evaluations. Our study aims to assess the necessity of this regular screening within a predominantly pediatric NF1 population by examining the occurrence of ocular adverse events (OAE). A retrospective study evaluated 45 NF1 patients receiving MEKi. Inclusion criteria included baseline and follow-up examinations following the initiation of MEKi therapy. At each assessment, a comprehensive eye evaluation was performed, comprising a dilated fundus examination, ocular coherence tomography of the macula and nerve fiber layer, and Humphrey visual field testing. Twenty-six patients, with an average age of 13 years (range 2–23 years) and an average follow-up duration of 413 days were included in the analysis. Three different MEKi were used: selumetinib (77%), trametinib (23%), and mirdametinib (4%). None of the patients experienced retinopathy at any point during the study. Some patients had pre-existing optic neuropathies (27%), but no instances of nerve changes occurred after commencing MEKi therapy. Four patients (15%) exhibited symptoms of dry eye, all of which were effectively managed with topical lubrication. Full article

Three-dimensional (3D) printing of polymer materials encompasses a wide range of applications and innovations. Polymer-based 3D printing, also known as additive manufacturing, has gained significant attention due to its versatility, cost-effectiveness, and potential to revolutionize various industries. The current paper focuses on obtaining a durable low-cost rehabilitation knee orthosis. Researchers propose that the entire structure should be obtained using modern equipment within the additive manufacturing domain—3D printing. The researchers focus on determining, through a 3D analysis of the entire 3D model assembly, which parts present a high degree of stress when a kinematic simulation is developed. The entire 3D model of the orthosis starts based on the result obtained from a 3D scanning of the knee joint of a patient, providing a precise fixation, and allowing for direct personalization. Based on the results and identification of the critical parts, there will be used different materials and a combination of 3D printing strategies to validate the physical model of the entire orthosis. For the manufacturing process, the researchers use two types of low-cost fused filament fabrication (FFF), which are easy to find on the worldwide market. The motivation for manufacturing the entire assembly using 3D printing techniques is the short time in which complex shapes can be obtained, which is relevant for the present study. The main purpose of the present research is to advance orthotic technology by developing an innovative knee brace made of 3D-printed polymers that are designed to be lightweight, easy-to-use, and provide comfort and functionality to patients during the rehabilitation process. Full article

Glyphosate, a widely used herbicide, is linked to a plethora of deleterious effects in both clinical and preclinical studies. Nevertheless, the effects of its main metabolite, aminomethylphosphonic acid (AMPA), whose half-life in soil is even longer than that of glyphosate, have been little explored. On this basis, as a first approach, in this work, we report that intraperitoneal (i.p.) administration of AMPA or glyphosate (at 10, 56, and 100 mg/kg) decreased, to a similar extent, plasma cholinesterase (ChE) activity in acutely exposed rats. Moreover, we designed an experimental protocol to analyze and compare the effects of AMPA and glyphosate on human plasma ChE activity; this protocol consisted of adding these compounds to human plasma to subsequently test the effects of this plasma on the contraction to acetylcholine (ACh) in the frog rectus abdominis muscle (an indirect estimate of ChE activity). Accordingly, this muscular contraction to ACh was evaluated before and after pre-incubation of ACh with (i) plasma alone, (ii) plasma with AMPA, and (iii) plasma with glyphosate. Our results indicate that AMPA, like glyphosate, decreased ChE activity in the plasma of rats (when given i.p.) and humans (when added in vitro), suggesting that both xenobiotics may exert similar toxicological effects. Full article

As bioindicators, benthic macroinvertebrates are often used to assess stream quality. Based on standard hydrobiological study techniques, the physicochemical and biological health status of the Missolé stream was assessed. Waters of the Missolé stream were found to be slightly acidic (pH: 6.23–6.26) and well-oxygenated (O₂: 69.80–76.80%), with low values of temperature (T°: 23.60–24° C), turbidity (49.40–88.40 FTU) and mineralized ions (NH₄⁺: 0–1.19 mg/L; NO₂^-: 0–1.61 mg/L; NO₃^-: 0.02–6.80 mg/L). Concerning aquatic invertebrate communities, a total of 489 individuals, grouped in two classes, eight orders and 35 families, all belonging to the phylum Arthropoda, were collected and identified. The class of Insecta was the most diversified, with seven orders and 32 families, while that of Crustacea had only one order and three families. Overall, Insecta accounted for 52.35% of the total abundance, and Decapod Crustacea was 47.65%. The three predominant families were Palaemonidae, Dytiscidae and Atyidae. Shannon and Weaver (H’) and Piélou’s evenness (J) indices were high at all stations and showed a slight decrease from upstream to downstream. In the same vein, the Hilsenhoff Biotic Index (HBI) classified the water quality of the Missolé stream as medium. Overall, this suburban aquatic ecosystem offers moderately favorable living conditions for aquatic biota. Full article