Background: The aim of this study was to evaluate the short-term efficacy and safety of the Paul Glaucoma Implant (PGI) in pediatric eyes diagnosed with glaucoma following congenital cataract surgery (GFCS). Methods: A retrospective, single-center, descriptive study was conducted on consecutive children diagnosed with GFCS who underwent PGI implantation between July 2022 and November 2023 at the University Medical Center Mainz. The primary outcome measure was the reduction in IOP at the last follow-up visit. Results: Ten eyes of nine children were included in the study. The mean follow-up time was 7.70 ± 4.22 months (4.68–10.72 months). At the end of the study follow-up, the mean (95% CI) reduction in IOP was −14.8 ± 8.73 mmHg (−8.56 to −21.04 mmHg, p < 0.001). At the last follow-up, 30.0% (3/10) of patients achieved an IOP (intraocular pressure) of ≥6 and ≤21 mmHg with a reduction in IOP of ≥25% without treatment, while 90.0% (9/10) achieved this target IOP regardless of glaucoma medication treatment. The mean number of antiglaucoma medications was significantly reduced from 3.50 (IQR = 1) to 2.0 (IQR = 2, p = 0.01), and the visual acuity logMAR improved from 1.26 ± 0.62 to 1.03 ± 0.48 (p = 0.04). Only one eye experienced numerical hypotony (4 mmHg) without choroidal detachment or anterior chamber shallowing within the first 24 h. No other adverse events were observed during the follow-up period. Conclusions: PGI implantation significantly lowered IOP and the number of antiglaucoma eye drops with a favorable safety profile in children diagnosed with GFCS, thereby achieving a high rate of qualified surgical success in the short term. Full article

Integrating wind power plants into the electricity grid poses challenges due to the intermittent nature of wind energy generation. Energy storage systems (ESSs) have shown promise in mitigating the intermittent variability associated with wind power. This paper presents a distributionally robust optimization (DRO) model for sizing energy storage systems to dispatch wind power plants. The variable wind power is formulated as a moment-based ambiguity set. Dispatchability is described by the expected value of the insufficient power of wind power relative to the dispatch command, which is a sum of nonlinear functions and is taken as the optimal index. A deterministic semi-definite positive model is derived to solve the problem effectively. Numerical studies are conducted to demonstrate the effectiveness and advantages of the proposed method. Full article

Unilateral condylar hyperplasia (UCH) is a rare cause of asymmetrical mandibular overgrowth because of the presence of an atypical growth in the affected condyle. SPECT (single-photon emission computed tomography) can easily establish the presence of an atypical, prolonged growth exceeding far beyond normal condylar growth and activity. A CT, CBCT, or LDCT (computed tomography, cone-beam computed tomography, or low-dose computed tomography) can confirm the diagnosis by evaluating the scope of bone overgrowth, mandibular basis/ramus asymmetry, tendency to condylar head enlargement, changes in bone density, and occurrence of differences in condylar head shapes, size, and bone structure. In most cases, a condylectomy is the procedure of choice in growing cases of UCH to remove the pathological condyle and reduce asymmetry levels. Sometimes, the growth is very slow and progressive over time, causing slowly growing asymmetry with similar symptoms to any other mandibular asymmetry, and this causes some troublesome procedures in UCH diagnostics, resulting in patients being underdiagnosed; it can even lead to some relapses in mandibular asymmetry and skeletal malocclusion after previously performed orthodontic and surgical treatment of such discrepancies. When the source of asymmetry is not identified in time, possible inadequate treatment protocols can be used. If any relapse of facial and mandibular asymmetry re-occur, SPECT and CT evaluation are necessary to evaluate if condylar hyperplasia is present and to establish what kind of surgical intervention should be used in each case. Full article

The Qaidam Block, located at the northern Qinghai–Tibet Plateau, is a pivotal area in unraveling the closure time of the Kunlun Ocean basin which might have recorded the transformation process between the Proto-Tethys and Paleo-Tethys Ocean basins. However, the late Triassic position of the Qaidam Block remains enigmatic, largely due to the scarcity of paleomagnetic data essential for quantitatively determining its paleolatitude. The widespread presence of the Elashan formation, particularly along the southern periphery of the Qaidam block, presents good material for conducting paleomagnetic work. Nevertheless, the primary magnetic carriers preserved within the Elashan formation might be influenced by multiple tectonic thermal events, particularly those associated with collisions between southern blocks and the Qaidam Block. Here we present rock magnetism and magnetic fabrics studies to identify the content and composition of magnetic minerals within the Elashan formation. The rock magnetic and petrologic results show that the magnetic carriers in the samples from the Elashan formation are dominated by magnetite with a small amount of goethite, pyrrhotite, and hematite. The results of Anisotropy in Magnetic Susceptibility indicate that the south of the Longwalangku section might not be obviously influenced by the tectonic events. Our results also provided guidance for future paleomagnetic research, emphasizing the importance of conducting further sampling away from adjacent faults, particularly in the southern Longwalangku area. Full article

Siderophores are a class of small molecules renowned for their high iron binding capacity, essential for all life forms requiring iron. This article provides a detailed review of the diverse classifications, and biosynthetic pathways of siderophores, with a particular emphasis on siderophores synthesized via nonribosomal peptide synthetase (NRPS) and non-NRPS pathways. We further explore the secretion mechanisms of siderophores in microbes and plants, and their role in regulating bioavailable iron levels. Beyond biological functions, the applications of siderophores in medicine, agriculture, and environmental sciences are extensively discussed. These applications include biological pest control, disease treatment, ecological pollution remediation, and heavy metal ion removal. Through a comprehensive analysis of the chemical properties and biological activities of siderophores, this paper demonstrates their wide prospects in scientific research and practical applications, while also highlighting current research gaps and potential future directions. Full article

Background: A negative lifestyle is reported to be related to cognitive problems. However, there is little information about this in relation to university students. The objective of the present study was to investigate the association between executive functions (EFs) and lifestyle parameters (i.e., physical activity (PA), sleep duration, screen time (ST), and food habits) among Chilean university students. Methods: This cross-sectional study included a total of 150 university students (94 females and 56 males, aged 21.28 ± 3.15 and 22.18 ± 2.90 years, respectively). Cognitive outcomes were measured using the CogniFit assessment battery. Lifestyle was measured through validated questionnaires. Results: Across the total sample, attention exhibited a positive association with PA h/week (β: 24.34 95% CI: 12.46 to 36.22, p = 0.001). Additionally, coordination was positively associated with PA h/week (β: 15.06 95% CI: 0.62 to 29.50, p < 0.041). PA h/week was positively linked with reasoning (β: 20.34 95% CI: 4.52 to 36.17, p = 0.012) and perception (β: 13.81 95% CI: 4.14 to 23.49, p = 0.005). Moreover, PA h/week was significantly linked to memory (β: 23.01 95% CI: 7.62 to 38.40, p = 0.004). In terms of the EFs, PA h/week showed a positive association with cognitive flexibility (β: 45.60 95% CI: 23.22 to 67.69, p = 0.001). Conclusions: In conclusion, lifestyle (PA h/week) was positively associated with EFs. Therefore, an increase in PA levels among these students should be a target for community- and university-based interventions in order to promote cognitive development such as attention, coordination, reasoning, perception, memory, and cognitive flexibility. Full article

The emergence of combination antiretroviral therapy (cART) has greatly transformed the life expectancy of people living with HIV (PWH). Today, over 76% of the individuals with HIV have access to this life-saving therapy. However, this progress has come with a new challenge: an increase in age-related non-AIDS conditions among patients with HIV. These conditions manifest earlier in PWH than in uninfected individuals, accelerating the aging process. Like PWH, the uninfected aging population experiences immunosenescence marked by an increased proinflammatory environment. This phenomenon is linked to chronic inflammation, driven in part by cellular structures called inflammasomes. Inflammatory signaling pathways activated by HIV-1 infection play a key role in inflammasome formation, suggesting a crucial link between HIV and a chronic inflammatory state. This review outlines the inflammatory processes triggered by HIV-1 infection and aging, with a focus on the inflammasomes. This review also explores current research regarding inflammasomes and potential strategies for targeting inflammasomes to mitigate inflammation. Further research on inflammasome signaling presents a unique opportunity to develop targeted interventions and innovative therapeutic modalities for combating HIV and aging-associated inflammatory processes. Full article

The Jerusalem artichoke (Helianthus tuberosus) is a high-yield crop, and a great source of fermentable sugars, which gives the plant the potential to be used as raw material for economical fuel alcohol production. In this article, the authors focus on the technological aspect of the biofuel manufacturing process and its properties. First, the fuel alcohol manufacturing process is described, afterwards assessing its characteristics such as kinematic viscosity, density and octane number. The amount of fuel alcohol obtained from 10 kg of biomass equals to 0.85 L. Afterwards, the mixtures of gasoline and obtained fuel alcohol are prepared and studied. Optimal alcohol and gasoline mixtures are determined to obtain biofuels with octane ratings of 92, 95 and 98. The kinematic viscosity of obtained mixtures does not differ significantly from its values for pure gasoline. The obtained biofuel mixture with 25% alcohol content yielded a decrease of sulfur content by 38%, an increase of vaporized fuel amount by 17.5% at 70 °C and by 10.5% at a temperature of 100 °C, which improves engine startup time and ensures its stable operation in comparison to pure gasoline. The alcohol obtained can be successfully used as a high-octane additive for gasolines. Full article

Multiple research directions have been proposed to study the information structure of Reddit. One of them is to model inter-subreddit relations but modeling user interactions in the form of a graph. Building upon prior work centered on political subreddits using pre-2020 data, we expand this investigation to include a more extensive dataset spanning 2022 and encompassing diverse topic areas. Employing NLP techniques such as text embeddings, we model subreddit content directly and construct a subreddit graph network based on cosine similarity. Community detection using the Louvain method reveals distinct subreddits and allows the analysis of inter-community connections via previous works’ concepts of “bridges” and “gateways”. Surprisingly, our findings indicate redundancy between bridges and gateways in the utilized dataset. Therefore, we introduce a new concept, “highways”. Highways, representing the most traversed paths between subreddits, unveil insights not captured by previous analyses, underscoring the significance of novel conceptual frameworks in uncovering latent knowledge within Reddit’s online community structures. Full article

This study aimed to compare the impact of CAD/CAM closed systems and open systems on the marginal gap of monolithic zirconia-reinforced lithium silicate (ZLS) ceramic crowns, as both systems are used in everyday dentistry, both chair-side and laboratory. For the closed system, 20 plastic teeth were scanned by a Primescan intra-oral scanner (IOS), and for the open system, the same number of plastic teeth were scanned by Trios 4 IOS. For the closed system, CEREC software was used, and for the open system, EXOCAD software was used. All 40 ZLS crowns were grinded by the same four-axis machine and cemented with Temp-bond, followed by self-adhesive resin cement. For each type of cement, an evaluation of the marginal gap was conducted by scanning electron microscopy (SEM). Before comparisons between the groups, a Kolmogorov–Smirnov test was performed on the study variables showing a normal distribution (p > 0.05). Independent T tests (α = 0.05) and paired-sample T tests (α = 0.05) were used. The independent T test found no significant mean marginal gap differences in the zirconia-reinforced lithium silicate crowns bonded with Temp-bond and scanned by Primescan (28.09 μm ± 3.06) compared to Trios 4 (28.94 μm ± 3.30) (p = 0.401), and there was no significant mean marginal gap differences in zirconia-reinforced lithium silicate crowns bonded with self-adhesive resin cement (Gcem ONE) and scanned by Primescan (46.70 μm ± 3.80) compared to Trios 4 (47.79 μm ± 2.59) (p = 0.295). Paired-sample T tests showed significantly higher mean marginal gaps with Gcem ONE compared to Temp-bond for the total mean marginal gap when scanning with Primescan (p = 0.0005) or Trios 4 (p = 0.0005). In everyday dentistry, both closed systems (Primescan with Cerec) and open systems (Trios 4 with Exocad) can be used to achieve an acceptable (<120 µm) marginal gap for ZLS CELTRA^® DUO single crowns. There is a significant difference between cementation with Temp-bond and Gcem ONE self-adhesive resin cement (p < 0.05). Full article

Amino acids are organic compounds that enter the protein structure, being involved in the proper functioning of the body. The role of amino acids in the onset of autism spectrum disorder (ASD) is yet to be established. Our aim was to identify correlations between urine amino acids and their derivatives and ASD. Methods: We designed a case–control study that consisted of 75 boys and girls, aged between 2 and 12 years. For amino acid profile, we used urine samples that were analyzed using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Results: Descriptive analysis showed higher values for glutamine, hydroxyproline, tyrosine, aspartic acid, and tryptophan and lower values for serine in the autism group than in the control group. Also, we found that boys with autism had higher values than the boys in the control group for serine, threonine, and aspartic acid. For girls from both groups, we did not find statistically significant values. In terms of age groups, we found significantly higher values for histidine, threonine, valine, methionine, aspartic acid, glutamic acid, alpha amino-adipic acid, sarcosine, alanine, and beta-alanine and significantly lower values for proline for both the autism and control groups under 5 years. Conclusions: The findings of this study support the assumption that amino acids may have a role in the expression of ASD. Full article

The sustainability of the food production achieved with the help of irrigation systems and the sustainability of their energy consumption are major challenges of the current century. Pumping systems currently account for approximately 30% of global electrical energy consumption. As electricity prices rise, there is a growing need for technological advancements to enhance energy efficiency and reduce consumption costs effectively. This study focuses on operating centrifugal pumps at variable frequency as an effective means of achieving this goal. Most centrifugal pump manufacturers/providers traditionally assume that pump efficiency remains constant across various operating frequencies, often equating the efficiency at various frequencies to that at the standard frequency (50/60 Hz). In contrast, this paper introduces a new formula for estimating pump efficiency, crucial to precise power consumption determination, particularly in variable-frequency scenarios. The formula parameters are identified by using experimental data acquired from an existing pumping system. The tests and results presented in this paper demonstrate that the proposed formula outperforms the formulas of the current industry standards in accuracy. Practically, the new relation assures enhanced accuracy in estimating pump efficiency and absorbed power, allowing for the design of a more precise model used for control systems synthesis required for operating centrifugal pumps at variable frequency. This research offers a new way of calculating pump efficiency, which could be very useful for industry practitioners seeking to optimize energy consumption in pumping systems. Full article

Crosstalk regulation is widespread in Streptomyces species. Elucidating the influence of a specific regulator on target biosynthetic gene clusters (BGCs) and cell metabolism is crucial for strain improvement through regulatory protein engineering. PteF and PteR are two regulators that control the biosynthesis of filipin, which competes for building blocks with avermectins in Streptomyces avermitilis. However, little is known about the effects of PteF and PteR on avermectin biosynthesis. In this study, we investigated their impact on avermectin biosynthesis and global cell metabolism. The deletion of pteF resulted in a 55.49% avermectin titer improvement, which was 23.08% higher than that observed from pteR deletion, suggesting that PteF plays a more significant role in regulating avermectin biosynthesis, while PteF hardly influences the transcription level of genes in avermectin and other polyketide BGCs. Transcriptome data revealed that PteF exhibited a global regulatory effect. Avermectin production enhancement could be attributed to the repression of the tricarboxylic acid cycle and fatty acid biosynthetic pathway, as well as the enhancement of pathways supplying acyl-CoA precursors. These findings provide new insights into the role of PteF on avermectin biosynthesis and cell metabolism, offering important clues for designing and building efficient metabolic pathways to develop high-yield avermectin-producing strains. Full article

The use of autonomous and unmanned systems continues to increase, with uses spanning from package delivery to simple automation of tasks and from factory usage to defense industries and agricultural applications. With the proliferation of unmanned systems comes the question of how to secure the command-and-control communication links among such devices and their operators. In this work, we look at the use of the Messaging Layer Security (MLS) protocol, designed to support long-lived continuous sessions and group communication with a high degree of security. We build out MAUI—an MLS API for UxS Integration that provides an interface for the secure exchange of data between a ScanEagle unmanned aerial vehicle (UAV) and an unmanned surface vehicle (USV) in a multi-domain ad-hoc network configuration, and experiment on system limits such as the ciphersuite set-up time and message handling rates. The experiments in this work were conducted in virtual and physical environments between the UAV, USV, and a controller device (all of different platforms). Our results demonstrate the viability of capitalizing on MLS’s capabilities to securely and efficiently transmit data for distributed communication among various unmanned system platforms. Full article

Healthcare human resource planning is one of the biggest challenges facing the healthcare systems in many countries. Inadequate decisions in human resource planning can lead to an insufficient number of healthcare professionals then healthcare inequalities. One of the components of resource planning in the healthcare system is long-term data monitoring and the identification of potential trends. Since 1990, the number of physicians in Lithuania has decreased by 15.3% (−2266), but the decrease in the population has led to a 13.61% increase in the number of physicians per 10,000 inhabitants (5.32). During the analyzed period, the largest decrease in the number of physicians workforce by specialty was the number of medical physicians (−73.08%), epidemiology and hygiene (−69.30%), children’s diseases (−49.08%), the most increased number was of family/general practitioners (GPs), geneticists, physical medicine, and rehabilitation specialists. Since 1992, the number of visits to physicians in Lithuania, which has been decreasing for a long time, began increasing, and in 2022 (9.3 visits) it has almost reached the number of visits (9.5) per capita as in 1991. The aim of this research was to collect long-term data from various databases, summarize them, and identify possible trends and the reasons for data changes. The study analyzed data from the Lithuanian healthcare system from the Declaration of Independence of Lithuania to the last 30 years. The data includes or affects the indicators of the healthcare system, changes in population and doctors, the number of visits to doctors, the number of medical students and residents, and data determining inequalities in the healthcare system. Long-term data analysis is useful for developing a model of healthcare human resource planning and for planning healthcare resources. Full article

The gasification of residues into syngas offers a versatile gaseous fuel that can be used to produce heat and power in various applications. However, the application of syngas in engines presents several challenges due to the changes in its composition. Such variations can significantly alter the optimal operational conditions of the engines that are fueled with syngas, resulting in combustion instability, high engine variability, and misfires. In this context, this work presents an experimental investigation conducted on a port-fuel injection spark-ignition optical research engine using three different syngas mixtures, with a particular focus on the effects of CO/H₂ and diluent ratios. A comparative analysis is made against methane, considered as the baseline fuel. The in-cylinder pressure and related parameters are examined as indicators of combustion behavior. Additionally, 2D cycle-resolved digital visualization is employed to trace flame front propagation. Custom image processing techniques are applied to estimate flame speed, displacement, and morphological parameters. The engine runs at a constant speed (900 rpm) and with full throttle like stationary engine applications. The excess air–fuel ratios vary from 1.0 to 1.4 by adjusting the injection time and the spark timing according to the maximum brake torque of the baseline fuel. A thermodynamic analysis revealed notable trends in in-cylinder pressure traces, indicative of differences in combustion evolution and peak pressures among the syngas mixtures and methane. Moreover, the study quantified parameters such as the mass fraction burned, combustion stability (COVIMEP), and fuel conversion efficiency. The analysis provided insights into flame morphology, propagation speed, and distortion under varying conditions, shedding light on the influence of fuel composition and air dilution. Overall, the results contribute to advancing the understanding of syngas combustion behavior in SI engines and hold implications for optimizing engine performance and developing numerical models. Full article

Vitamin D₃ is a steroid hormone that confers anti-tumorigenic properties in prostate cells. Serum vitamin D₃ deficiency has been associated with advanced prostate cancer (PCa), particularly affecting African American (AA) men. Therefore, elucidating the pleiotropic effects of vitamin D on signaling pathways, essential to maintaining non-malignancy, may provide additional drug targets to mitigate disparate outcomes for men with PCa, especially AA men. We conducted RNA sequencing on an AA non-malignant prostate cell line, RC-77N/E, comparing untreated cells to those treated with 10 nM of vitamin D₃ metabolite, 1α,25(OH)₂D₃, at 24 h. Differential gene expression analysis revealed 1601 significant genes affected by 1α,25(OH)₂D₃ treatment. Pathway enrichment analysis predicted 1α,25(OH)₂D_3- mediated repression of prostate cancer, cell proliferation, actin cytoskeletal, and actin-related signaling pathways (p < 0.05). Prioritizing genes with vitamin D response elements and associating expression levels with overall survival (OS) in The Cancer Genome Atlas Prostate Adenocarcinoma (TCGA PRAD) cohort, we identified ANLN (Anillin) and ECT2 (Epithelial Cell Transforming 2) as potential prognostic PCa biomarkers. Both genes were strongly correlated and significantly downregulated by 1α,25(OH)₂D₃ treatment, where low expression was statistically associated with better overall survival outcomes in the TCGA PRAD public cohort. Increased ANLN and ECT2 mRNA gene expression was significantly associated with PCa, and Gleason scores using both the TCGA cohort (p < 0.05) and an AA non-malignant/tumor-matched cohort. Our findings suggest 1α,25(OH)₂D₃ regulation of these biomarkers may be significant for PCa prevention. In addition, 1α,25(OH)₂D₃ could be used as an adjuvant treatment targeting actin cytoskeleton signaling and actin cytoskeleton-related signaling pathways, particularly among AA men. Full article

The generation of high-quality wideband frequency sweeps presents a significant challenge, particularly in modern telecommunication, radar, and measurement systems where miniaturization is paramount. While phase-locked loops (PLLs) have become the dominant technique for signal generation, their application in broadband sweeps necessitates fractional-N operation. This, in turn, degrades phase noise and introduces unwanted spurs. This paper proposes a novel approach for broadband signal generation. By cascading two PLLs and utilizing a coaxial resonator, we achieve a high-performance oscillator that operates without the excessive fractional spurs, maintaining their level below −80 dBc across the entire frequency band. The prototype demonstrates non-degraded phase noise performance, reaching −102 dBc/Hz at 100 kHz offset and −121 dBc/Hz at 1 MHz offset for signals at 10 GHz. Despite significant frequency jumps, our design achieves lock times below 41 µs. These results, supported by theoretical analysis, validate the proposed method’s effectiveness in generating low-noise broadband frequency sweeps, ideal for local oscillator applications. Full article

Breast cancer represents a significant health concern, particularly in Saudi Arabia, where it ranks as the most prevalent cancer type among women. This study focuses on leveraging eXplainable Artificial Intelligence (XAI) techniques to predict benign and malignant breast cancer cases using various clinical and pathological features specific to Saudi Arabian patients. Six distinct models were trained and evaluated based on common performance metrics such as accuracy, precision, recall, F1 score, and AUC-ROC score. To enhance interpretability, Local Interpretable Model-Agnostic Explanations (LIME) and SHapley Additive exPlanations (SHAP) were applied. The analysis identified the Random Forest model as the top performer, achieving an accuracy of 0.72, along with robust precision, recall, F1 score, and AUC-ROC score values. Conversely, the Support Vector Machine model exhibited the poorest performance metrics, indicating its limited predictive capability. Notably, the XAI approaches unveiled variations in the feature importance rankings across models, underscoring the need for further investigation. These findings offer valuable insights into breast cancer diagnosis and machine learning interpretation, aiding healthcare providers in understanding and potentially integrating such technologies into clinical practices. Full article

In the manufacturing sector, tool wear substantially affects product quality and production efficiency. While traditional sequential deep learning models can handle time-series tasks, their neglect of complex temporal relationships in time-series data often leads to errors accumulating in continuous predictions, which reduces their forecasting accuracy for tool wear. For addressing these limitations, the parallel convolutional and recurrent neural networks with attention-modulated residual learning (ParaCRN-AMResNet) model is introduced. Compared with conventional deep learning models, ParaCRN-AMResNet markedly enhances the efficiency and precision of feature extraction from time-series data through its innovative parallel architecture. The model adeptly combines dilated convolution neural network and bidirectional gated recurrent units, effectively addressing distance dependencies and enriching the quantity and dimensions of extracted features. The strength of ParaCRN-AMResNet lies in its refined ability to capture the complex dynamics of time-series data, significantly boosting the model’s accuracy and generalization capability. The model’s efficacy was validated through comprehensive milling experiments and vibration signal analyses, showcasing ParaCRN-AMResNet’s superior performance. In evaluation metrics, the model achieved a MAE of 2.6015, MSE of 15.1921, R² of 0.9897, and MAPE of 2.7997%, conclusively proving its efficiency and accuracy in the precise prediction of tool wear. Full article

Cover crops in no-tillage systems may alter soil mineral N and influence the N fertilizer use efficiency (NFUE) of subsequent maize. The hypothesis of this work is that no-tillage systems with cover crops affect nitrate, ammonium and maize NFUE availability in the Brazilian Cerrado. The objective was to evaluate the cover crop mineralization effect on soil N mineral and maize NFUE in a no-tillage system, with and without N topdressing. The experiment was arranged in a randomized block split-plot design. The plots were represented by cover crops (Cajanus cajan, Crotalaria juncea, Raphanus sativus and Mucuna aterrima). The subplots consisted of the application (WN) or non-application (NN) of N topdressing to maize. The soil was sampled in six layers (up to 60 cm) at the end (April) and at the beginning of the rainy season (November). NH₄⁺ was lower for all cover crops and WN and NN management in April. NO₃⁻ differed between seasons and cover crops in WN and NN. The lignin concentration and N uptake of M. aterrima were the highest compared to other species. The highest NFUE was on R. sativus, showing higher fertilizer dependency. In a no-tillage system with cover crops, the N topdressing fertilization needs to be improved, considering mineralization. Full article

The emergence of CD19-directed chimeric antigen receptor T-cell (CAR-T) therapy has revolutionized the treatment paradigm for R/R B-cell NHLs. However, challenges persist in accurately evaluating treatment response and detecting early relapse, necessitating the exploration of novel biomarkers. Circulating tumor DNA (ctDNA) via liquid biopsy is a non-invasive tool for monitoring therapy efficacy and predicting treatment outcomes in B-NHL following CAR-T therapy. By overcoming the limitations of conventional imaging modalities, ctDNA assessments offer valuable insights into response dynamics, molecular mechanisms of resistance, and early detection of molecular relapse. Integration of ctDNA monitoring into clinical practice holds promise for personalized therapeutic strategies, guiding the development of novel targeted therapies, and enhancing patient outcomes. However, standardization of assay methodologies and consensus on clinical response metrics are imperative to unlock the full potential of ctDNA in the management of B-NHL. Prospective validation of ctDNA in clinical trials is necessary to establish its role as a complementary decision aid. Full article