The 2023 MDPI Annual Report has
been released!
 
16 pages, 6070 KiB  
Article
RsRbohD1 Plays a Significant Role in ROS Production during Radish Pithiness Development
by Qiong Gong, Chaonan Wang, Weiqiang Fan, Shuiling Li, Hong Zhang, Zhiyin Huang, Xiaohui Liu, Ziyun Ma, Yong Wang and Bin Zhang
Plants 2024, 13(10), 1386; https://doi.org/10.3390/plants13101386 (registering DOI) - 16 May 2024
Abstract
Pithiness is one of the physiological diseases of radishes, which is accompanied by the accumulation of reactive oxygen species (ROS) during the sponging of parenchyma tissue in the fleshy roots. A respiratory burst oxidase homolog (Rboh, also known as NADPH oxidase) is a [...] Read more.
Pithiness is one of the physiological diseases of radishes, which is accompanied by the accumulation of reactive oxygen species (ROS) during the sponging of parenchyma tissue in the fleshy roots. A respiratory burst oxidase homolog (Rboh, also known as NADPH oxidase) is a key enzyme that catalyzes the production of ROS in plants. To understand the role of Rboh genes in radish pithiness, herein, 10 RsRboh gene families were identified in the genome of Raphanus sativus using Blastp and Hmmer searching methods and were subjected to basic functional analyses such as phylogenetic tree construction, chromosomal localization, conserved structural domain analysis, and promoter element prediction. The expression profiles of RsRbohs in five stages (Pithiness grade = 0, 1, 2, 3, 4, respectively) of radish pithiness were analyzed. The results showed that 10 RsRbohs expressed different levels during the development of radish pithiness. Except for RsRbohB and RsRbohE, the expression of other members increased and reached the peak at the P2 (Pithiness grade = 2) stage, among which RsRbohD1 showed the highest transcripts. Then, the expression of 40 genes related to RsRbohD1 and pithiness were analyzed. These results can provide a theoretical basis for improving pithiness tolerance in radishes. Full article
(This article belongs to the Special Issue The Growth and Development of Vegetable Crops)
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19 pages, 2976 KiB  
Article
Long-Term Energy System Modelling for a Clean Energy Transition in Egypt’s Energy Sector
by Anna Gibson, Zen Makuch, Rudolf Yeganyan, Naomi Tan, Carla Cannone and Mark Howells
Energies 2024, 17(10), 2397; https://doi.org/10.3390/en17102397 (registering DOI) - 16 May 2024
Abstract
Egypt has the potential to generate a significant amount of energy from renewable technologies, in particular solar PV, concentrated solar power (CSP), and onshore and offshore wind. The energy sector is reliant on fossil fuels, particularly natural gas, for electricity production and is [...] Read more.
Egypt has the potential to generate a significant amount of energy from renewable technologies, in particular solar PV, concentrated solar power (CSP), and onshore and offshore wind. The energy sector is reliant on fossil fuels, particularly natural gas, for electricity production and is at risk of locking itself into a high carbon pathway. Globally, reducing greenhouse gas (GHG) emissions associated with national energy sectors is a target outlined in the UN’s Paris Agreement. To reduce carbon dioxide (CO2) emissions associated with a higher dependence on fossil fuels, Egypt must consider upscaling renewable energy technologies (RETs) to achieve a clean energy transition (CET). This research modelled six scenarios using clicSAND for OSeMOSYS to identify the technologies and policy target improvements that are needed to upscale RETs within Egypt’s energy sector. The results showed that solar PV and onshore wind are key technologies to be upscaled to contribute towards Egypt’s CET. The optimal renewable target is the International Renewable Energy Agency’s (IRENA) target of 53% of electricity being sourced from RETs by 2030, which will cost USD 16.4 billion more up to 2035 than Egypt’s current Integrated Sustainable Energy Strategy (ISES) target of 42% by 2035; it also saves 732.0 MtCO2 over the entire modelling period to 2070. Socio-economic barriers to this transition are considered, such as recent discoveries of natural gas reserves combined with a history of energy insecurity, political instability impacting investor confidence, and a lack of international climate funding. The paper concludes with policy recommendations that would enable Egypt to progress towards achieving a CET. Full article
(This article belongs to the Collection Energy Transition towards Carbon Neutrality)
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12 pages, 4568 KiB  
Article
Comparison of the Effects of Pilates and Yoga Exercise on the Dynamic Balancing Ability and Functional Movement of Fencers
by So-Jung Lim, Hyun-Jin Kim, Yong-Soo Kim, Eunkuk Kim, Inyoung Hwang and Ju-Seop Kang
Life 2024, 14(5), 635; https://doi.org/10.3390/life14050635 (registering DOI) - 16 May 2024
Abstract
This study was conducted to compare and analyze whether Pilates exercise and yoga exercise help improve the performance of female fencers and prevent injury, and the dynamic balance test (LQ-YBT) and functional movement screening (FMS) test score of the elite adult female fencers [...] Read more.
This study was conducted to compare and analyze whether Pilates exercise and yoga exercise help improve the performance of female fencers and prevent injury, and the dynamic balance test (LQ-YBT) and functional movement screening (FMS) test score of the elite adult female fencers were compared and analyzed as evaluation indicators. Participants were randomly classified into Pilates (n = 10) and yoga groups (n = 10), members of which took part in 50 min of exercise (5 min of warm-up, 40 min of main exercise, and 5 min of cool-down) twice weekly for eight weeks. The results obtained from this study were analyzed via independent t-test and 2-way ANOVA. The results were as follows: LQ-YBT measures (reaching distance) increased significantly for both groups, as did FMS scores (deep squat, hurdle step, inline lunge, shoulder mobility, active straight-leg raise, trunk-stability push-up, and rotary stability). These results suggest that Pilates exercise and yoga exercise might be likely effective in improving the performance of adult female fencers and injury prevention by increasing their dynamic balance ability and functional movement. Full article
(This article belongs to the Special Issue Effects of Exercise Training on Muscle Function)
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14 pages, 286 KiB  
Article
The Effect of Organizational Ethical Climate on Job Satisfaction among Nurses: The Mediating Role of Trust in Colleagues
by Özlem Köroğlu, İrfan Akkoç, Ömer Turunç, Abdullah Çalışkan, Müzeyyen Çiğdem Akbaş, Cem Karayalçın, Güney Gürsel and Halil Akbaş
Sustainability 2024, 16(10), 4179; https://doi.org/10.3390/su16104179 (registering DOI) - 16 May 2024
Abstract
This study examines the effect of the ethical climate on job satisfaction in the nursing profession and how this effect occurs through trust in colleagues. A survey design was used to collect data from 407 nurses working in hospitals in Mersin City, Turkey. [...] Read more.
This study examines the effect of the ethical climate on job satisfaction in the nursing profession and how this effect occurs through trust in colleagues. A survey design was used to collect data from 407 nurses working in hospitals in Mersin City, Turkey. The study used self-report questionnaires to measure the ethical climate, job satisfaction, and trust. The data were analyzed using SPSS 22 software, which incorporated confirmatory factor analysis to assess construct validity and the bootstrap method to examine mediation effects. Ethical protocols were rigorously observed. The study clearly demonstrated a significant positive relationship between the ethical climate and job satisfaction. The trust variable fully mediates the relationship between the ethical climate and job satisfaction. The findings make a strong case for the importance of an ethical climate in nursing, synthesizing findings from the previous literature. The findings of this study demonstrate the necessity of assessing different attitudes and behaviors in order to promote expected behaviors in the unique and intense working environment of nurses. These behaviors may diverge from theoretical predictions. The results of this study also revealed that an ethical climate and positive work attitudes among nurses have a significant effect on job satisfaction, which is an important input for productivity. Full article
15 pages, 588 KiB  
Systematic Review
Clinical Outcomes and Molecular Predictors of Pembrolizumab (Keytruda) as a PD-1 Immune Checkpoint Inhibitor in Advanced and Metastatic Cervical Cancer: A Systematic Review and Meta-Analysis
by Lavinia Balan, Anca Maria Cimpean, Prashant Sunil Nandarge, Bogdan Sorop, Catalin Balan, Madalina Alexandra Balica, Felix Bratosin, Simona Brasoveanu, Madalina Boruga and Laurentiu Pirtea
Biomedicines 2024, 12(5), 1109; https://doi.org/10.3390/biomedicines12051109 (registering DOI) - 16 May 2024
Abstract
This systematic review evaluates the clinical outcomes and molecular predictors of response to pembrolizumab in patients with advanced and metastatic cervical cancer. We adhered to the PRISMA guidelines for systematic reviews, conducting a database search in PubMed, Scopus, and Embase. The eligibility criteria [...] Read more.
This systematic review evaluates the clinical outcomes and molecular predictors of response to pembrolizumab in patients with advanced and metastatic cervical cancer. We adhered to the PRISMA guidelines for systematic reviews, conducting a database search in PubMed, Scopus, and Embase. The eligibility criteria centered on clinical outcomes, including the overall survival (OS), progression-free survival (PFS), and immune-related biomarkers post-pembrolizumab therapy. We included both prospective and retrospective studies that detailed clinical outcomes and molecular characteristics predictive of therapeutic response. Our search yielded six studies involving 846 patients treated with pembrolizumab from 2017 to 2022. The meta-analysis of these studies showed that pembrolizumab, used as monotherapy or in combination with chemotherapy, extended the OS by a weighted median of 10.35 months and the PFS by 8.50 months. The treatment demonstrated a pooled objective response rate (ORR) of 22.39%, although the I2 test result of 67.49% showed a high heterogeneity among the studies. Notably, patients with high PD-L1 expression (CPS ≥ 10) experienced improved outcomes in terms of the PFS and OS. The most common complications were fatigue, diarrhea, and immune-related adverse events. Pembrolizumab significantly enhances clinical outcomes in metastatic cervical cancer, particularly among patients with high PD-L1 expression. The drug maintains a good safety profile, reinforcing its treatment potential for patients with advanced and metastatic cervical cancer. Future studies should explore long-term effects and strategies to integrate pembrolizumab optimally into current treatment regimens, aiming to maximize patient benefits and effectively manage side effects. Full article
(This article belongs to the Special Issue Drug Resistance and Novel Targets for Cancer Therapy—Second Edition)
18 pages, 1097 KiB  
Article
Effects of Mediterranean Diet, Curcumin, and Resveratrol on Mild-to-Moderate Active Ulcerative Colitis: A Multicenter Randomized Clinical Trial
by Özge Erol Doğan, Kezban Esen Karaca Çelik, Murat Baş, Eyüp Hakan Alan and Yasir Furkan Çağın
Nutrients 2024, 16(10), 1504; https://doi.org/10.3390/nu16101504 (registering DOI) - 16 May 2024
Abstract
This study aimed to investigate the effects of the Mediterranean diet (MD), combined with curcumin and resveratrol supplementation, on disease activity, serum inflammatory markers, and quality of life in patients with mild-to-moderate active ulcerative colitis (UC). This study was designed as a prospective [...] Read more.
This study aimed to investigate the effects of the Mediterranean diet (MD), combined with curcumin and resveratrol supplementation, on disease activity, serum inflammatory markers, and quality of life in patients with mild-to-moderate active ulcerative colitis (UC). This study was designed as a prospective multicenter three-arm randomized controlled trial. Participants were randomized to the MD, MD + curcumin, and MD + resveratrol groups. All participants were placed on the MD for 8 weeks. The MD + curcumin group also received 1600 mg/day of curcumin supplementation, whereas the MD + resveratrol group received 500 mg/day of resveratrol supplementation for 8 weeks. Anthropometric measurements, Truelove–Witts Index, Short Form-36, Inflammatory Bowel Disease Questionnaire, Mediterranean Diet Adherence Scale (MEDAS), and laboratory tests were performed at baseline and postintervention. Within-group comparisons showed that MD, MD + curcumin, and MD + resveratrol interventions were effective in reducing disease activity and inflammation and improving quality of life in individuals with UC (p < 0.05). Between-group comparisons revealed no significant difference in all parameters except for the pain subparameter of SF-36 and the MEDAS score (p < 0.05). The MD is an effective and safe intervention to be used in clinical practice in individuals with UC. Full article
(This article belongs to the Section Phytochemicals and Human Health)
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37 pages, 2887 KiB  
Article
A Study on Powder Spreading Quality in Powder Bed Fusion Processes Using Discrete Element Method Simulation
by Panagiotis Avrampos and George-Christopher Vosniakos
J. Manuf. Mater. Process. 2024, 8(3), 101; https://doi.org/10.3390/jmmp8030101 (registering DOI) - 16 May 2024
Abstract
Powder deposition is a very important aspect of PBF-based additive manufacturing processes. Discrete Element Method (DEM) is commonly utilized by researchers to examine the physically complex aspects of powder-spreading methods. This work focuses on vibration-assisted doctor blade powder recoating. The aim of this [...] Read more.
Powder deposition is a very important aspect of PBF-based additive manufacturing processes. Discrete Element Method (DEM) is commonly utilized by researchers to examine the physically complex aspects of powder-spreading methods. This work focuses on vibration-assisted doctor blade powder recoating. The aim of this work is to use experiment-verified DEM simulations in combination with Taguchi Design of Experiments (DoE) to identify optimum spreading parameters based on robust layer quality criteria. The verification of the used powder model is performed via angle of repose and angle of avalanche simulation–experiment cross-checking. Then, four criteria, namely layer thickness deviation, surface coverage ratio, surface root-mean-square roughness and true packing density, are defined. It has been proven that the doctor blade’s translational speed plays the most important role in defining the quality of the deposited layer. The true packing density was found to be unaffected by the spreading parameters. The vertical vibration of the doctor blade recoater was found to have a beneficial effect on the quality of the deposited layer. Ultimately, a weighted mean quality criteria analysis is mapped out. Skewness and kurtosis were proven to function as effective indicators of layer quality, showing a linear relation to the weighted means of the defined quality criteria. The specific weights that optimize this linearity were identified. Full article
17 pages, 735 KiB  
Article
Pulsed Propulsion of Unmanned Aerial Vehicles by Centrifugal Force Modulation—First-Order Theory and Practicability
by Wolfgang Holzapfel
Appl. Sci. 2024, 14(10), 4229; https://doi.org/10.3390/app14104229 (registering DOI) - 16 May 2024
Abstract
A novel technique suitable for the propulsion of small unmanned aerial vehicles (UAV) is discussed in this paper. This approach utilizes the rotational energy of airborne gyro rotors and converts it into translational propulsion for the vehicle. The energy conversion is achieved by [...] Read more.
A novel technique suitable for the propulsion of small unmanned aerial vehicles (UAV) is discussed in this paper. This approach utilizes the rotational energy of airborne gyro rotors and converts it into translational propulsion for the vehicle. The energy conversion is achieved by generating precisely directed centrifugal force pulses through short-duration rotor unbalances. The accurate control of the timing and magnitude of these unbalances is crucial for successful propulsion generation. Our first-order theory of controlled unbalance propulsion (CUP) predicts the potential for achieving high translational accelerations and vehicle velocities up to orbital levels. Power-saving levitation of UAVs can be attained. In this paper, we provide traceable evidence that pulsed centrifugal propulsion is based on well-established laws of physics and can be realized using state-of-the-art technologies. Full article
(This article belongs to the Section Aerospace Science and Engineering)
13 pages, 433 KiB  
Article
Personalized Feedback in Massive Open Online Courses: Harnessing the Power of LangChain and OpenAI API
by Miguel Morales-Chan, Hector R. Amado-Salvatierra, José Amelio Medina, Roberto Barchino, Rocael Hernández-Rizzardini and António Moreira Teixeira
Electronics 2024, 13(10), 1960; https://doi.org/10.3390/electronics13101960 (registering DOI) - 16 May 2024
Abstract
Studies show that feedback greatly improves student learning outcomes, but achieving this level of personalization at scale is a complex task, especially in the diverse and open environment of Massive Open Online Courses (MOOCs). This research provides a novel method for using cutting-edge [...] Read more.
Studies show that feedback greatly improves student learning outcomes, but achieving this level of personalization at scale is a complex task, especially in the diverse and open environment of Massive Open Online Courses (MOOCs). This research provides a novel method for using cutting-edge artificial intelligence technology to enhance the feedback mechanism in MOOCs. The main goal of this research is to leverage AI’s capabilities to automate and refine the MOOC feedback process, with special emphasis on courses that allow students to learn at their own pace. The combination of LangChain—a cutting-edge framework specifically designed for applications that use language models—with the OpenAI API forms the basis of this work. This integration creates dynamic, scalable, and intelligent environments that can provide students with individualized, insightful feedback. A well-organized assessment rubric directs the feedback system, ensuring that the responses are both tailored to each learner’s unique path and aligned with academic standards and objectives. This initiative uses Generative AI to enhance MOOCs, making them more engaging, responsive, and successful for a diverse, international student body. Beyond mere automation, this technology has the potential to transform fundamentally how learning is supported in digital environments and how feedback is delivered. The initial results demonstrate increased learner satisfaction and progress, thereby validating the effectiveness of personalized feedback powered by AI. Full article
14 pages, 3457 KiB  
Article
Structure Identification of Ganoderma lucidum Spore Polysaccharides and Their Antitumor Activity In Vivo
by Hui-Min Liu, Jun Cheng, Xiao-Yi Wang, Yan Jiang, Jia Ni, Yun Zhang and Wei Wang
Molecules 2024, 29(10), 2348; https://doi.org/10.3390/molecules29102348 (registering DOI) - 16 May 2024
Abstract
Ganoderma lucidum spore powder, valued for its nutritional and medicinal properties, contains polysaccharides crucial for its efficacy. However, the complex structural nature of these polysaccharides necessitates further investigation to fully realize their potential. This study aimed to investigate the effects of acid heat [...] Read more.
Ganoderma lucidum spore powder, valued for its nutritional and medicinal properties, contains polysaccharides crucial for its efficacy. However, the complex structural nature of these polysaccharides necessitates further investigation to fully realize their potential. This study aimed to investigate the effects of acid heat treatment on Ganoderma lucidum spore polysaccharides (GLSPs) to enhance their properties and application in antitumor activity. The GLSP was obtained via acid heat treatment, concentration, and centrifugal separation. This process led to a notable reduction in polysaccharide molecular weight, increasing water solubility and bioavailability. Analytical techniques including NMR spectroscopy and methylation analysis revealed a polysaccharide composition comprising four distinct monosaccharides, with molecular weights of 3291 Da (Mw) and 3216 Da (Mn). Six different linkage modes were identified, with a molar ratio of 1:5:2:3:4:3. In vivo experiments demonstrated the GLSP’s significant inhibitory effect on the growth of four tumor models (sarcoma S180, Lewis lung cancer, liver cancer H22, and colon cancer C26) in mice, with no observed toxicity. These findings suggest the GLSP’s potential as an antitumor therapeutic agent for clinical use. Full article
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17 pages, 4515 KiB  
Article
Efficacy of Glycyrrhetinic Acid in the Treatment of Acne Vulgaris Based on Network Pharmacology and Experimental Validation
by Lingna Xie, Congwei Ma, Xinyu Li, Huixiong Chen, Ping Han, Li Lin, Weiqiang Huang, Menglu Xu, Hailiang Lu and Zhiyun Du
Molecules 2024, 29(10), 2345; https://doi.org/10.3390/molecules29102345 (registering DOI) - 16 May 2024
Abstract
Glycyrrhetinic acid (GA) is a saponin compound, isolated from licorice (Glycyrrhiza glabra), which has been wildly explored for its intriguing pharmacological and medicinal effects. GA is a triterpenoid glycoside displaying an array of pharmacological and biological activities, including anti-inflammatory, anti-bacterial, antiviral and antioxidative [...] Read more.
Glycyrrhetinic acid (GA) is a saponin compound, isolated from licorice (Glycyrrhiza glabra), which has been wildly explored for its intriguing pharmacological and medicinal effects. GA is a triterpenoid glycoside displaying an array of pharmacological and biological activities, including anti-inflammatory, anti-bacterial, antiviral and antioxidative properties. In this study, we investigated the underlying mechanisms of GA on acne vulgaris through network pharmacology and proteomics. After the intersection of the 154 drug targets and 581 disease targets, 37 therapeutic targets for GA against acne were obtained. A protein–protein interaction (PPI) network analysis highlighted TNF, IL1B, IL6, ESR1, PPARG, NFKB1, STAT3 and TLR4 as key targets of GA against acne, which is further verified by molecular docking. The experimental results showed that GA inhibited lipid synthesis in vitro and in vivo, improved the histopathological damage of skin, prevented mast cell infiltration and decreased the level of pro-inflammatory cytokines, including TNF-α, IL-1β and IL-6. This study indicates that GA may regulate multiple pathways to improve acne symptoms, and the beneficial effects of GA against acne vulgaris might be through the regulation of sebogenesis and inflammatory responses. Full article
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12 pages, 6416 KiB  
Article
An Intelligent Detection System for Wheat Appearance Quality
by Junling Liang, Jianpin Chen, Meixuan Zhou, Heng Li, Yiheng Xu, Fei Xu, Liping Yin and Xinyu Chai
Agronomy 2024, 14(5), 1057; https://doi.org/10.3390/agronomy14051057 (registering DOI) - 16 May 2024
Abstract
In the realm of commercial trade, the appearance quality of wheat is a crucial metric for assessing its value and grading. Traditionally, evaluating wheat appearance quality is a manual process conducted by inspectors, which is time-consuming, laborious, and error-prone. In this research, we [...] Read more.
In the realm of commercial trade, the appearance quality of wheat is a crucial metric for assessing its value and grading. Traditionally, evaluating wheat appearance quality is a manual process conducted by inspectors, which is time-consuming, laborious, and error-prone. In this research, we developed an intelligent detection system for wheat appearance quality, leveraging state-of-the-art neural network technology for the efficient and standardized assessment of wheat appearance quality. Our system was meticulously crafted, integrating high-performance hardware components and sophisticated software solutions. Central to its functionality is a detection model built upon multi-grained convolutional neural networks. This innovative setup allows for the swift and precise evaluation and categorization of wheat quality. Remarkably, our system achieved an exceptional overall recognition accuracy rate of 99.45% for wheat grain categories, boasting a recognition efficiency that was approximately five times faster than manual recognition processes. This groundbreaking system serves as a valuable tool for assisting inspectors, offering technical support for customs quarantine, grain reserves, and food safety. Full article
(This article belongs to the Special Issue In-Field Detection and Monitoring Technology in Precision Agriculture)
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14 pages, 731 KiB  
Article
Acidic Electrolyzed Water Maintains the Storage Quality of Postharvest Wampee Fruit by Activating the Disease Resistance
by Yuzhao Lin, Hongbin Chen, Sisi Dong, Yazhen Chen, Xuanjing Jiang and Yihui Chen
Foods 2024, 13(10), 1556; https://doi.org/10.3390/foods13101556 (registering DOI) - 16 May 2024
Abstract
Harvested wampee fruit is susceptible to disease, resulting in postharvest losses. Acidic electrolyzed water (AEW), a safe and innovative sterilization technology, plays a role in enhancing disease resistance in harvested produce. In this study, the efficacy of AEW in delaying wampee disease development [...] Read more.
Harvested wampee fruit is susceptible to disease, resulting in postharvest losses. Acidic electrolyzed water (AEW), a safe and innovative sterilization technology, plays a role in enhancing disease resistance in harvested produce. In this study, the efficacy of AEW in delaying wampee disease development was assessed, along with its association with disease resistance metabolism. Wampee fruit was treated with AEW (pH 2.5) at different available chlorine concentrations (ACCs) (20, 40, 60, and 80 mg/L) and subsequently stored at 25 °C for 8 days. Results revealed that 40 mg/L ACC in AEW (pH 2.5) was most effective in improving the postharvest quality of wampee fruit. Compared with control wampee fruit, those treated with 40 mg/L ACC in AEW exhibited lower incidence of fruit disease, higher pericarp lignin content, and higher activities of pericarp disease resistance enzymes (DREs), such as cinnamate-4-hydroxylase, phenylalanine ammonia-lyase, chitinase, β-1,3-glucanase, polyphenol oxidase, 4-coumarate CoA ligase, and cinnamyl alcohol dehydrogenase. These results suggested that AEW elevated DRE activities, promoted lignin accumulation, and ultimately enhanced disease resistance, suppressed disease development, and improved storage quality in harvested wampee fruit. Consequently, AEW emerged as a safe technology to mitigate the disease development and enhance the storage quality of harvested wampee fruit. Full article
10 pages, 519 KiB  
Communication
The Effect of Combined Application of Biocontrol Microorganisms and Arbuscular Mycorrhizal Fungi on Plant Growth and Yield of Tomato (Solanum lycopersicum L.)
by Alaa Abdulkadhim A. Almuslimawi, Borbála Kuchár, Susana Estefania Araujo Navas, György Turóczi and Katalin Posta
Agriculture 2024, 14(5), 768; https://doi.org/10.3390/agriculture14050768 (registering DOI) - 16 May 2024
Abstract
Sustainable plant production requires less use of synthetic chemicals in plant nutrition and protection. Microbial products are among the most promising substitutes for chemicals. With the increasing popularity and availability of such products, it has become obligatory to use different microbes together. The [...] Read more.
Sustainable plant production requires less use of synthetic chemicals in plant nutrition and protection. Microbial products are among the most promising substitutes for chemicals. With the increasing popularity and availability of such products, it has become obligatory to use different microbes together. The effect of this has been tested in several studies, but their results have sometimes been contradictory depending on the microbial strains tested and the mode of application. We tested the effect of two commercially available antagonists and Funneliformis mosseae alone and in combination on tomato. Mycorrhizal treatment increased plant growth and yield, both alone and combined with the antagonists; however, mycorrhizal root colonization was not influenced by the antagonist. This treatment also led to a slight decrease in the occurrence of Trichoderma spp. on tomato roots but did not impede the colonization of roots by the applied Trichoderma strain. Our result confirmed that Trichoderma asperellum (T34) and Streptomyces griseoviridis (K61) can be safely combined with arbuscular mycorrhizal fungi (AMF), namely with F. mosseae. Full article
(This article belongs to the Special Issue Advanced Research of Rhizosphere Microbial Activity—Series II)
10 pages, 348 KiB  
Article
Osteopontin as a Biomarker in Interstitial Lung Diseases
by David Iturbe-Fernández, Verónica Pulito-Cueto, Víctor M. Mora-Cuesta, Sara Remuzgo-Martínez, Diego J. Ferrer-Pargada, Fernanda Genre, Pilar Alonso-Lecue, Raquel López-Mejías, Belén Atienza-Mateo, Miguel A. González-Gay and José M. Cifrián-Martínez
Biomedicines 2024, 12(5), 1108; https://doi.org/10.3390/biomedicines12051108 (registering DOI) - 16 May 2024
Abstract
Osteopontin (OPN) is a glycoprotein involved in Th1 and Th17 differentiation, and inflammation and tissue remodeling. OPN is a biomarker of disease activity in patients with autoimmune inflammatory conditions. This study aimed to assess the diagnostic and prognostic value of OPN in interstitial [...] Read more.
Osteopontin (OPN) is a glycoprotein involved in Th1 and Th17 differentiation, and inflammation and tissue remodeling. OPN is a biomarker of disease activity in patients with autoimmune inflammatory conditions. This study aimed to assess the diagnostic and prognostic value of OPN in interstitial lung diseases (ILDs). Between May 2016 and October 2019, 344 patients with ILD were recruited at the Hospital Universitario Marqués de Valdecilla (Spain) and were prospectively followed-up. This study involved the determination of OPN serum levels by ELISA and OPN RNA expression quantified using qPCR. Six genetic polymorphisms in OPN (rs28357094, rs2853749, rs2853750, rs11728697, rs7695531, and rs1126616) were genotyped using TaqMan assays. OPN serum levels were also assessed in 140 healthy controls. OPN serum levels (median [interquartile range]) were significantly higher in ILD patients than in controls (1.05 [0.75–1.51] ng/mL versus 0.81 [0.65–0.98] ng/mL in healthy controls; p < 0.01). OPN serum levels were inversely correlated with the forced vital capacity. OPN serum levels were also higher in ILD patients who died or underwent lung transplantation when compared with the remaining ILD patients (1.15 [0.80–1.72] ng/mL versus 0.99 [0.66–1.32] ng/mL; p = 0.05). Survival worsened in ILD patients with OPN > 1.03 ng/mL at 1, 3, and 5 years. No statistically significant differences in the genetic frequencies of OPN polymorphisms or the RNA expression were found among the different ILD groups. Elevated levels of OPN in the serum may be a useful indicator in identifying patients with ILD who are more likely to experience poor outcomes. Full article
(This article belongs to the Section Molecular Genetics and Genetic Diseases)
12 pages, 1201 KiB  
Article
Orientation-Dependent Mechanical Behaviors of BCC-Fe in Light of the Thermo-Kinetic Synergy of Plastic Deformation
by Yu Liu, Jinglian Du, Kunyu Zhang, Kangxu Gao, Haotian Xue, Xiao Fang, Kexing Song and Feng Liu
Materials 2024, 17(10), 2395; https://doi.org/10.3390/ma17102395 (registering DOI) - 16 May 2024
Abstract
The orientation-dependent mechanical behaviors of metallic alloys are governed by deformation mechanisms, but the underlying physics remain to be explored. In this work, the mechanical responses along different orientations and behind the mechanisms of BCC-Fe are investigated by performing molecular dynamic simulations. It [...] Read more.
The orientation-dependent mechanical behaviors of metallic alloys are governed by deformation mechanisms, but the underlying physics remain to be explored. In this work, the mechanical responses along different orientations and behind the mechanisms of BCC-Fe are investigated by performing molecular dynamic simulations. It is found that the mechanical properties of BCC-Fe exhibit apparent anisotropic characteristics. The <100>-oriented BCC-Fe presents a Young’s modulus of E = 147.56 GPa, a strength of σy = 10.15 GPa, and a plastic strain of εy = 0.084 at the yield point, whereas the <111> orientation presents E = 244.84 GPa, σy = 27.57 GPa, and εy = 0.21. Based on classical dislocation theory, the reasons for such orientation-dependent mechanical behaviors are analyzed from the perspective of thermo-kinetic synergy upon deformation. It turns out that the anisotropic mechanical responses of BCC-Fe are associated with the magnitude of the thermodynamic driving force (ΔG) and kinetic energy barrier (Q) for dislocation motion, which dominate the corresponding deformation mechanism. Compared with the low ΔG (6.395 GPa) and high Q (11.95 KJ/mol) of the <100>-oriented BCC-Fe dominated by deformation twinning, the <111> orientation governed by dislocation slip presents a high ΔG (17.37 GPa) and low Q (6.45 KJ/mol). Accordingly, the orientation-dependent deformation behaviors of BCC-Fe are derived from the thermo-kinetic synergy for dislocation motion. Full article
16 pages, 2059 KiB  
Article
Growth of Quasi-Two-Dimensional CrTe Nanoflakes and CrTe/Transition Metal Dichalcogenide Heterostructures
by Dawei Cheng, Jiayi Liu and Bin Wei
Nanomaterials 2024, 14(10), 868; https://doi.org/10.3390/nano14100868 (registering DOI) - 16 May 2024
Abstract
Two-dimensional (2D) van der Waals layered materials have been explored in depth. They can be vertically stacked into a 2D heterostructure and represent a fundamental way to explore new physical properties and fabricate high-performance nanodevices. However, the controllable and scaled growth of non-layered [...] Read more.
Two-dimensional (2D) van der Waals layered materials have been explored in depth. They can be vertically stacked into a 2D heterostructure and represent a fundamental way to explore new physical properties and fabricate high-performance nanodevices. However, the controllable and scaled growth of non-layered quasi-2D materials and their heterostructures is still a great challenge. Here, we report a selective two-step growth method for high-quality single crystalline CrTe/WSe2 and CrTe/MoS2 heterostructures by adopting a universal CVD strategy with the assistance of molten salt and mass control. Quasi-2D metallic CrTe was grown on pre-deposited 2D transition metal dichalcogenides (TMDC) under relatively low temperatures. A 2D CrTe/TMDC heterostructure was established to explore the interface’s structure using scanning transmission electron microscopy (STEM), and also demonstrate ferromagnetism in a metal–semiconductor CrTe/TMDC heterostructure. Full article
(This article belongs to the Special Issue Nano-Structured Thin Films: Growth, Characteristics, and Application)
20 pages, 625 KiB  
Article
Using Optimization Techniques in Grammatical Evolution
by Ioannis G. Tsoulos, Alexandros Tzallas and Evangelos Karvounis
Future Internet 2024, 16(5), 172; https://doi.org/10.3390/fi16050172 (registering DOI) - 16 May 2024
Abstract
The Grammatical Evolution technique has been successfully applied to a wide range of problems in various scientific fields. However, in many cases, techniques that make use of Grammatical Evolution become trapped in local minima of the objective problem and fail to reach the [...] Read more.
The Grammatical Evolution technique has been successfully applied to a wide range of problems in various scientific fields. However, in many cases, techniques that make use of Grammatical Evolution become trapped in local minima of the objective problem and fail to reach the optimal solution. One simple method to tackle such situations is the usage of hybrid techniques, where local minimization algorithms are used in conjunction with the main algorithm. However, Grammatical Evolution is an integer optimization problem and, as a consequence, techniques should be formulated that are applicable to it as well. In the current work, a modified version of the Simulated Annealing algorithm is used as a local optimization procedure in Grammatical Evolution. This approach was tested on the Constructed Neural Networks and a remarkable improvement of the experimental results was shown, both in classification data and in data fitting cases. Full article
29 pages, 974 KiB  
Article
Monitoring the Phenolic and Terpenic Profile of Olives, Olive Oils and By-Products throughout the Production Process
by Lucía López-Salas, Javier Díaz-Moreno, Marco Ciulu, Isabel Borrás-Linares, Rosa Quirantes-Piné and Jesús Lozano-Sánchez
Foods 2024, 13(10), 1555; https://doi.org/10.3390/foods13101555 (registering DOI) - 16 May 2024
Abstract
Olive oil is a food of great importance in the Mediterranean diet and culture. However, during its production, the olive oil industry generates a large amount of waste by-products that can be an important source of bioactive compounds, such as phenolic compounds and [...] Read more.
Olive oil is a food of great importance in the Mediterranean diet and culture. However, during its production, the olive oil industry generates a large amount of waste by-products that can be an important source of bioactive compounds, such as phenolic compounds and terpenes, revalorizing them in the context of the circular economy. Therefore, it is of great interest to study the distribution and abundance of these bioactive compounds in the different by-products. This research is a screening focused on phytochemical analysis, with particular emphasis on the identification and quantification of the phenolic and terpenic fractions. Both the main products of the olive industry (olives, olive paste and produced oil) and the by-products generated throughout the oil production process (leaf, “alpeorujo”, liquid and solid residues generated during decanting commonly named “borras” and washing water) were analyzed. For this purpose, different optimized extraction procedures were performed for each matrix, followed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-TOF/MS) analysis. Although no phenolic alcohols were quantified in the leaf and the presence of secoiridoids was low, this by-product was notable for its flavonoid (720 ± 20 µg/g) and terpene (5000 ± 300 µg/g) contents. “Alpeorujo” presented a complete profile of compounds of interest, being abundant in phenolic alcohols (900 ± 100 µg/g), secoiridoids (4500 ± 500 µg/g) and terpenes (1200 ± 100 µg/g), among others. On the other hand, while the solid residue of the borras was the most abundant in phenolic alcohols (3700 ± 200 µg/g) and secoiridoids (680 ± 20 µg/g), the liquid fraction of this waste was notable for its content of elenolic acid derivatives (1700 ± 100 µg/mL) and phenolic alcohols (3000 ± 300 µg/mL). Furthermore, to our knowledge, this is the first time that the terpene content of this by-product has been monitored, demonstrating that it is an important source of these compounds, especially maslinic acid (120 ± 20 µg/g). Finally, the phytochemical content in wash water was lower than expected, and only elenolic acid derivatives were detected (6 ± 1 µg/mL). The results highlighted the potential of the olive by-products as possible alternative sources of a wide variety of olive bioactive compounds for their revalorization into value-added products. Full article
(This article belongs to the Special Issue Bioactive Compounds, Antioxidants, and Health Benefits—Volume II)
23 pages, 4983 KiB  
Article
Study on Water and Salt Transport Characteristics of Sunflowers under Different Irrigation Amounts in the Yellow River Irrigation Area
by Changfu Tong, Rui He, Jun Wang and Hexiang Zheng
Agronomy 2024, 14(5), 1058; https://doi.org/10.3390/agronomy14051058 (registering DOI) - 16 May 2024
Abstract
The control of irrigation volume is of significant importance in arid regions of northwest China. Particularly, it has a crucial impact on the salinization of shallow groundwater areas. In 2022 and 2023, field experiments were conducted to test three distinct under-membrane irrigation treatments. [...] Read more.
The control of irrigation volume is of significant importance in arid regions of northwest China. Particularly, it has a crucial impact on the salinization of shallow groundwater areas. In 2022 and 2023, field experiments were conducted to test three distinct under-membrane irrigation treatments. These treatments were assigned water quotas of HW (27 mm), MW (22.5 mm), and LW (18 mm). The HYDRUS-2D model was integrated with a field experiment to accurately simulate the dynamic fluctuations of soil water and salt in the sunflower root zone. The model’s performance was assessed and verified using real-field data from 2022 and 2023, and the simulation results closely matched the measured values. This research also used stable hydroxide isotopes to assess the water supply from various soil layers at different time intervals in sunflower plants. The results indicated that the three different levels of irrigation applied under the membrane had a significant impact on soil water content. Specifically, there was a significant difference in soil water content at a depth of 0–40 cm (p < 0.05), while there was little effect on the water content at a depth of 40–60 cm (p > 0.05). After irrigation, the average salt content in the top 0–20 cm of soil decreased by 7.0% compared to the medium and low irrigation levels, and by 10.8% compared to the medium irrigation level. Additionally, the medium irrigation level resulted in a 10.8% decrease in salt content compared to the low irrigation level, and a 4.1% decrease compared to the medium irrigation level. During the same period, the soil salinity levels at depths of 0–20 cm, 20–40 cm, 40–60 cm, and 60–100 cm in the area outside the membrane were measured to be 2.7~4.8 g·kg−1, 2.8~4.0 g·kg−1, 2.7~3.4 g·kg−1, and 1.7~2.6 g·kg−1, respectively. These levels decreased by 13.1~55.5%, 0.7~42.8%, −0.4~16.2%, and −72.7~7.5%, respectively. Following irrigation, the HW treatment mostly absorbed water in the 0–40 cm soil layer, while the MW and LW treatments absorbed water in both the 0–40 cm and 60–80 cm soil levels. The results indicated that the most optimal drip irrigation method beneath the membrane in this location was achieved when the amount of water applied was between 25–30 mm. This method demonstrated a combination of water conservation, high crop yield, and effective salt suppression. Full article
28 pages, 5027 KiB  
Article
Assessing Soil Prediction Distributions for Forest Management Using Digital Soil Mapping
by Gonzalo Gavilán-Acuna, Nicholas C. Coops, Guillermo F. Olmedo, Piotr Tompalski, Dominik Roeser and Andrés Varhola
Soil Syst. 2024, 8(2), 55; https://doi.org/10.3390/soilsystems8020055 (registering DOI) - 16 May 2024
Abstract
Texture, soil organic matter (SOM), and soil depth (SoD) are crucial properties in forest management because they can supply spatial information on forest site productivity and guide fertilizer applications. However, soil properties possess an inherent uncertainty that must be mapped to enhance decision [...] Read more.
Texture, soil organic matter (SOM), and soil depth (SoD) are crucial properties in forest management because they can supply spatial information on forest site productivity and guide fertilizer applications. However, soil properties possess an inherent uncertainty that must be mapped to enhance decision making in management applications. Most digital soil mapping predictions primarily concentrate on the mean of the distribution, often neglecting the estimation of local uncertainty in soil properties. Additionally, there is a noticeable scarcity of practical soil examples to demonstrate the prediction uncertainty for the benefit of forest managers. In this study, following a digital soil mapping (DSM) approach, a Quantile Regression Forest (QRF) model was developed to generate high-resolution maps and their uncertainty regarding the texture, SoD, and SOM, which were expressed as standard deviation (Sd) values. The results showed that the SOM (R2 = 0.61, RMSE = 2.03% and with an average Sd = 50%), SoD (R2 = 0.74 and RMSE = 19.4 cm), clay (R2 = 0.63, RMSE = 10.5% and average Sd = 29%), silt (R2 = 0.59, RMSE = 6.26% and average Sd = 33%), and sand content (R2 = 0.55, RMSE = 9.49% and average Sd = 35%) were accurately estimated for forest plantations in central south Chile. A practical demonstration of precision fertilizer application, utilizing the predictive distribution of SOM, effectively showcased how uncertainty in soil attributes can be leveraged to benefit forest managers. This approach holds potential for optimizing resource allocation and maximizing economic benefits. Full article
(This article belongs to the Special Issue Contemporary Applications of Geostatistics to Soil Studies)
12 pages, 608 KiB  
Article
Incisor and Soft Tissue Characteristics of Adult Bimaxillary Protrusion Patients among Different Skeletal Anteroposterior Classifications
by Thitirat Siangloy and Chairat Charoemratrote
Diagnostics 2024, 14(10), 1031; https://doi.org/10.3390/diagnostics14101031 (registering DOI) - 16 May 2024
Abstract
The objective of this study was to investigate the upper incisors (U1), lower incisors (L1), and soft tissue profiles of bimaxillary protrusion (BM) adult patients among skeletal Class I (BM1), II (BM2) and III (BM3). Understanding these characteristics would be useful for incisor [...] Read more.
The objective of this study was to investigate the upper incisors (U1), lower incisors (L1), and soft tissue profiles of bimaxillary protrusion (BM) adult patients among skeletal Class I (BM1), II (BM2) and III (BM3). Understanding these characteristics would be useful for incisor and lip diagnostics in different skeletal classifications. Fourteen linear and twelve angular variables of the incisors and lips were evaluated in 214 lateral cephalograms (BM1 = 91, BM2 = 84, BM3 = 39). ANOVA and Bonferroni tests compared the measurements. BM1 and BM3 exhibited a greater U1 position and U1 inclination than the norms, while BM2 presented only a greater U1 position than the norms but normal U1 inclination. BM1 and BM3 had a significantly greater U1 position than BM2. BM1 and BM2 demonstrated a greater L1 position and L1 inclination than the norms, whereas BM3 displayed only a greater L1 position than the norms but normal L1 inclination. BM2 had the most anterior L1 position, whereas BM3 had the least anterior position. Only BM2 had a longer anterior dental height (ADH) than the norms, while BM1 and BM3 had a normal ADH and the significantly shortest ADH, respectively. Only BM1 had a normal upper incisor display at rest (U1R), while BM2 and BM3 displayed an increased and decreased U1R, respectively, with significant differences among the three groups. The most significantly protruded upper and lower lips were presented in BM2, but these were exhibited the least in BM3. The most significant acute nasolabial angle (NLA) was found in BM3, whereas BM2 presented the least acute NLA. A normal lip–chin–throat angle (LCTA) was observed in BM1 and BM3, while only BM2 had a greater LCTA than the norms. The most significant obtuse LCTA was found in BM2, while BM3 had the least obtuse LCTA. Therefore, both U1 and L1 in all groups presented protrusion and proclination, except for U1 in BM2, while L1 in BM3 exhibited normal inclination. The ADH and U1R were increased in BM2 but decreased in BM3. The most acute NLA was found in BM3, whereas the least acute was found in BM2. The most obtuse LCTA was in BM2, while the least was in BM3. Full article
(This article belongs to the Section Pathology and Molecular Diagnostics)
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22 pages, 36250 KiB  
Article
Physical and Numerical Experimentation of Water Droplet Collision on a Wall: A Comparison between PLIC and HRIC Schemes for the VOF Transport Equation with High-Speed Imaging
by Bruno Silva de Lima, Martin Sommerfeld and Francisco José de Souza
Fluids 2024, 9(5), 117; https://doi.org/10.3390/fluids9050117 (registering DOI) - 16 May 2024
Abstract
Liquid films are often found in engineering applications with thicknesses ranging from micrometer scales to large scales with a wide range of applications. To optimize such systems, researchers have dedicated themselves to the development of new techniques. To further contribute to this development, [...] Read more.
Liquid films are often found in engineering applications with thicknesses ranging from micrometer scales to large scales with a wide range of applications. To optimize such systems, researchers have dedicated themselves to the development of new techniques. To further contribute to this development, the objective of this work is to present the results of the collision of water droplets on a wall by means of experimentation and numerical simulations. For physical experimentation, an injector is used to generate a chain of water droplets that collide with the opposite wall, forming a liquid film. Images of the droplets were obtained using two high-speed recording cameras. The results for different droplet sizes and impact angles are presented and the relationship between the momentum parameter and non-dimensional pool size was established. Modeling such processes is a common challenge in engineering, with different techniques having their advantages and limitations. The simulations in this work were run using the volume of fluid method, which consists of solving a transport equation for the volume fraction of each considered fluid. A correlation was found between the surface tension to momentum transport ratio, Scd, and the non-dimensional pool size for different droplet sizes and impact angles. Regions where partial depositions were most likely to occur were found via physical experiments. Full article
(This article belongs to the Special Issue Multiphase Flow and Granular Mechanics)

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