The 2023 MDPI Annual Report has
been released!
 
14 pages, 4951 KiB  
Article
Tribological Properties of Aluminium Matrix Syntactic Foams Manufactured with Aluminium Smelter Waste
by Bhavik Mehta and Yuyuan Zhao
Appl. Sci. 2024, 14(10), 4288; https://doi.org/10.3390/app14104288 (registering DOI) - 18 May 2024
Abstract
Aluminium smelter waste (ASW) is a big contributor to landfills, and its recycling has been of great interest. This study investigates the tribological properties of aluminium matrix syntactic foams manufactured using an Al 6082 alloy and ASW. Ball-on-disc tests were conducted under both [...] Read more.
Aluminium smelter waste (ASW) is a big contributor to landfills, and its recycling has been of great interest. This study investigates the tribological properties of aluminium matrix syntactic foams manufactured using an Al 6082 alloy and ASW. Ball-on-disc tests were conducted under both dry and lubricated conditions. Under dry sliding conditions, the coefficient of friction (COF) had an initial sharp increase, followed by a gradual decrease and finally a steady state as the sliding distance increased. The wear surfaces showed the presence of adhesive, abrasive and oxidative wear, with some presence of delamination. Syntactic foams containing small ASW particles led to a decrease in surface roughness, decrease in the average COF and decrease in specific wear. Heating large ASW particles before manufacturing the syntactic foams enhanced overall wear properties because the particles are hardened due to a compositional change. The T6 treatment of the syntactic foams enhanced the wear properties due to the hardening of the Al matrix. The average COF of the ASW syntactic foams was higher than that of the E-sphere syntactic foam, which was predominantly abrasive wear. The specific wear of the ASW syntactic foams can be higher or lower than the E-sphere syntactic foam, depending on the ASW particle size. Under lubricated sliding test conditions, the wear was reduced significantly, and the type changed from predominantly adhesive to predominantly abrasive. The porous ASW particles acted as lubricant reservoirs and provided a constant supply of lubricant, further improving the lubrication effect. Full article
15 pages, 411 KiB  
Article
Prisoners’ Perceptions and Satisfaction with Telepsychiatry Services in Greece and the Effects of Its Use on the Coercion of Mental Healthcare
by Evangelia Karachaliou, Phoebe Douzenis, Fotios Chatzinikolaou, Nikos Pantazis, Sophia Martinaki, Panagiota Bali, Konstantinos Tasios and Athanasios Douzenis
Healthcare 2024, 12(10), 1044; https://doi.org/10.3390/healthcare12101044 (registering DOI) - 18 May 2024
Abstract
Background: Prisoners are often associated with mental health and substance use disorders. Coercive measures are widely used in prison settings. The objective of this study was to compare inmates’ perceptions and satisfaction with telepsychiatry versus face-to-face consultation and the effects of telepsychiatry on [...] Read more.
Background: Prisoners are often associated with mental health and substance use disorders. Coercive measures are widely used in prison settings. The objective of this study was to compare inmates’ perceptions and satisfaction with telepsychiatry versus face-to-face consultation and the effects of telepsychiatry on the use of coercive measures. The sample consisted of 100 male inmates from various backgrounds who had experienced both approaches of services (face to face and telepsychiatry). Method: The data were obtained through an interview where the individuals completed a Demographic Data Questionnaire, a Participant Satisfaction Questionnaire to assess satisfaction with face-to-face psychiatric services, and a Participant Satisfaction Questionnaire to assess their satisfaction with services offered via telepsychiatry. Additionally, calculations of time spent waiting for a face-to-face psychiatric evaluation and time spent in handcuffs and in confined spaces were made before and after the introduction of telepsychiatry. Results: Statistically significant improvements (all p-values < 0.001) were noted in waiting times, support for relapse prevention, follow up, quality of mental health care, quality of care in the management of psychiatric problems and related medication, behavior of psychiatrists, duration of the assessment, sense of comfort, and confidentiality. Telepsychiatry led to the elimination of time spent in handcuffs and in confined spaces (transport vehicles). Conclusion: According to the results of this study, telepsychiatry is an acceptable method of service delivery in correctional facilities and was associated with a reduction of coercive practices. Full article
(This article belongs to the Special Issue Moving Away from Coercion in Mental Health Care)
30 pages, 15928 KiB  
Article
Optimization of Ultrasound-Assisted Extraction of Free Phenolic Compounds and In Vitro Biological Activity from Peach Fruit Using Response Surface Methodology
by Dasha Mihaylova, Margarita Terziyska, Ivelina Desseva, Aneta Popova and Anna Lante
Appl. Sci. 2024, 14(10), 4286; https://doi.org/10.3390/app14104286 (registering DOI) - 18 May 2024
Abstract
In this study, the ultrasonic extraction (UAE) of free phenolic compounds and relative biological activities of the Bulgarian peach variety “Filina” was optimized using chemometric techniques (response surface methodology). A Box–Behnken design was used to reveal the variation in the hydro module, temperature, [...] Read more.
In this study, the ultrasonic extraction (UAE) of free phenolic compounds and relative biological activities of the Bulgarian peach variety “Filina” was optimized using chemometric techniques (response surface methodology). A Box–Behnken design was used to reveal the variation in the hydro module, temperature, duration, and extractant on the total phenolic content, total flavonoid content, antioxidant potential, and inhibitory activity on each yield. The results revealed that the optimal conditions included a hydro module of 20, a duration of 39.33 min, a temperature of 70 °C, and an extractant of 96.64% to retrieve the highest level of bioactive compounds. The calculated parameters were discovered to be following the projected values. Full article
22 pages, 2870 KiB  
Article
Whale Optimization for Cloud–Edge-Offloading Decision-Making for Smart Grid Services
by Gabriel Ioan Arcas, Tudor Cioara and Ionut Anghel
Biomimetics 2024, 9(5), 302; https://doi.org/10.3390/biomimetics9050302 (registering DOI) - 18 May 2024
Abstract
As IoT metering devices become increasingly prevalent, the smart energy grid encounters challenges associated with the transmission of large volumes of data affecting the latency of control services and the secure delivery of energy. Offloading computational work towards the edge is a viable [...] Read more.
As IoT metering devices become increasingly prevalent, the smart energy grid encounters challenges associated with the transmission of large volumes of data affecting the latency of control services and the secure delivery of energy. Offloading computational work towards the edge is a viable option; however, effectively coordinating service execution on edge nodes presents significant challenges due to the vast search space making it difficult to identify optimal decisions within a limited timeframe. In this research paper, we utilize the whale optimization algorithm to decide and select the optimal edge nodes for executing services’ computational tasks. We employ a directed acyclic graph to model dependencies among computational nodes, data network links, smart grid energy assets, and energy network organization, thereby facilitating more efficient navigation within the decision space to identify the optimal solution. The offloading decision variables are represented as a binary vector, which is evaluated using a fitness function considering round-trip time and the correlation between edge-task computational resources. To effectively explore offloading strategies and prevent convergence to suboptimal solutions, we adapt the feedback mechanisms, an inertia weight coefficient, and a nonlinear convergence factor. The evaluation results are promising, demonstrating that the proposed solution can effectively consider both energy and data network constraints while enduring faster decision-making for optimization, with notable improvements in response time and a low average execution time of approximately 0.03 s per iteration. Additionally, on complex computational infrastructures modeled, our solution shows strong features in terms of diversity, fitness evolution, and execution time. Full article
(This article belongs to the Special Issue Biomimetics and Bioinspired Artificial Intelligence Applications)
10 pages, 1248 KiB  
Article
Comparative Genome-Wide Alternative Splicing Analysis between Preadipocytes and Adipocytes
by Zhongyi Hou, Xin Li, Maosheng Xu, Shengbo Meng, Huifen Xu, Ming Li and Hanfang Cai
Genes 2024, 15(5), 640; https://doi.org/10.3390/genes15050640 (registering DOI) - 18 May 2024
Abstract
Alternative splicing is a ubiquitous regulatory mechanism in gene expression that allows a single gene to generate multiple messenger RNAs (mRNAs). Adipocyte development is regulated by many processes, and recent studies have found that splicing factors also play an important role in adipogenic [...] Read more.
Alternative splicing is a ubiquitous regulatory mechanism in gene expression that allows a single gene to generate multiple messenger RNAs (mRNAs). Adipocyte development is regulated by many processes, and recent studies have found that splicing factors also play an important role in adipogenic development. In the present study, we further investigated the differences in selective shearing during different periods of adipocyte differentiation. We identified five alternative splicing types including skipped exon, mutually exclusive exon, Alternative 5’ splice site, Alternative 3’ splice site, and Retained intron, with skipped exons being the most abundant type of selective shearing. In total, 641 differentially expressed selective shearing genes were obtained, enriched in 279 pathways, from which we selected and verified the accuracy of the sequencing results. Overall, RNA-seq revealed changes in the splicing and expression levels of these new candidate genes between precursor adipocytes and adipocytes, suggesting that they may be involved in adipocyte generation and differentiation. Full article
(This article belongs to the Special Issue Research on Genetics and Genomics of Cattle)
9 pages, 826 KiB  
Brief Report
Indulging Curiosity: Preliminary Evidence of an Anxiolytic-like Effect of Castor Oil and Ricinoleic Acid
by Khalin E. Nisbett, Leandro F. Vendruscolo and George F. Koob
Nutrients 2024, 16(10), 1527; https://doi.org/10.3390/nu16101527 (registering DOI) - 18 May 2024
Abstract
In the process of validating the elevated zero maze, a common test of anxiety-like behavior, in our laboratory, we demonstrated an anxiolytic-like effect of castor oil and its primary component, ricinoleic acid. We tested the effects of vehicle and chlordiazepoxide in male mice [...] Read more.
In the process of validating the elevated zero maze, a common test of anxiety-like behavior, in our laboratory, we demonstrated an anxiolytic-like effect of castor oil and its primary component, ricinoleic acid. We tested the effects of vehicle and chlordiazepoxide in male mice in the elevated zero maze following a 30-min pretreatment time. Chlordiazepoxide is a United States Food and Drug Administration-approved drug that was previously shown to exert anxiolytic-like effects in both the elevated zero maze and elevated plus maze. Chlordiazepoxide was administered at doses of 5 or 10 mg/kg. We used 5% polyoxyl 35 castor oil (Kolliphor® EL) and saline as treatment vehicles and found that the effect of chlordiazepoxide on open zone occupancy and open zone entries was blunted when 5% Kolliphor was used as the vehicle. These tests demonstrated that chlordiazepoxide increased open zone occupancy and entries in the elevated zero maze more effectively when saline was used as the treatment vehicle and that Kolliphor dampened the anxiolytic-like effect of chlordiazepoxide when it was used as the treatment vehicle. Notably, 5% Kolliphor alone slightly increased baseline open zone occupancy and entries. Given that Kolliphor is a derivative of castor oil, we next tested the effect of 5% castor oil and 5% ricinoleic acid, which is a major component of castor oil. We found that both castor oil and ricinoleic acid increased open zone occupancy but not entries compared with saline. Altogether, our findings demonstrate that Kolliphor, castor oil, and ricinoleic acid may exert anxiolytic-like effects in male mice in the elevated zero maze. This potential anxiolytic-like effect of castor oil is consistent with its well-established beneficial effects, including anti-inflammatory, antioxidant, antifungal, and pain-relieving properties. Full article
(This article belongs to the Section Phytochemicals and Human Health)
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17 pages, 19375 KiB  
Article
Deep Blind Fault Activity—A Fault Model of Strong Mw 5.5 Earthquake Seismogenic Structures in North China
by Guanshen Liu, Renqi Lu, Dengfa He, Lihua Fang, Yang Zhang, Peng Su and Wei Tao
Remote Sens. 2024, 16(10), 1796; https://doi.org/10.3390/rs16101796 (registering DOI) - 18 May 2024
Abstract
North China is one of the high-risk areas for destructive and strong earthquakes in mainland China and has experienced numerous strong historical earthquakes. An earthquake of magnitude MW 5.5 struck Pingyuan County, Dezhou city, in Shandong Province, China, on 6 August 2023. [...] Read more.
North China is one of the high-risk areas for destructive and strong earthquakes in mainland China and has experienced numerous strong historical earthquakes. An earthquake of magnitude MW 5.5 struck Pingyuan County, Dezhou city, in Shandong Province, China, on 6 August 2023. This earthquake was the strongest in the eastern North China Craton since the 1976 Tangshan earthquake. Since the earthquake did not produce surface ruptures, the seismogenic structure for fault responsible for the Pingyuan MW 5.5 earthquake is still unclear. To reveal the subsurface geological structure near the earthquake epicenter, this study used high-resolution two-dimensional (2D) seismic reflection profiles and constructed a three-dimensional (3D) geometric model of the Tuqiao Fault by interpreting the faults in the seismic reflection profiles. This study further combined focal mechanism solutions, aftershock clusters, and other seismological data to discuss the seismogenic fault of the Pingyuan MW 5.5 earthquake. The results show that the Tuqiao Fault is not the seismogenic fault of the MW 5.5 earthquake. The actual seismogenic structure may be related to the NE-oriented high-angle strike-slip blind fault developed in the basement. We further propose three possible fault models for the strong seismogenic structure in North China to discuss the potential seismotectonics in this region. Full article
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25 pages, 667 KiB  
Review
Food Insecurity in Greece and across the Globe: A Narrative Literature Review
by Emmanouil Alexandros Fotakis, Ioanna Kontele, Milia Tzoutzou, Maria G. Grammatikopoulou, Eirini Arvanitaki, Theodoros N. Sergentanis, Konstantinos Kotrokois, Eleni Kornarou and Tonia Vassilakou
Foods 2024, 13(10), 1579; https://doi.org/10.3390/foods13101579 (registering DOI) - 18 May 2024
Abstract
Food insecurity comprises a major global public health threat, as its effects are detrimental to the mental, physical, and social aspects of the health and well-being of those experiencing it. We performed a narrative literature review on the magnitude of global food insecurity [...] Read more.
Food insecurity comprises a major global public health threat, as its effects are detrimental to the mental, physical, and social aspects of the health and well-being of those experiencing it. We performed a narrative literature review on the magnitude of global food insecurity with a special emphasis on Greece and analyzed the major factors driving food insecurity, taking into consideration also the effect of the COVID-19 pandemic. An electronic search of international literature was conducted in three databases. More than 900 million people worldwide experience severe food insecurity, with future projections showing increasing trends. Within Europe, Eastern and Southern European countries display the highest food insecurity prevalence rates, with Greece reporting a prevalence of moderate or severe food insecurity ranging between 6.6% and 8% for the period 2019–2022. Climate change, war, armed conflicts and economic crises are major underlying drivers of food insecurity. Amidst these drivers, the COVID-19 pandemic had a profound impact on food insecurity levels around the globe, through halting economic growth, disrupting food supply chains and increasing unemployment and poverty. Tackling food insecurity through addressing its key drivers is essential to any progress towards succeeding the Sustainable Development Goal of “Zero Hunger”. Full article
(This article belongs to the Section Food Security and Sustainability)
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14 pages, 1757 KiB  
Article
Antibacterial and Antibiofilm Potential of Ethanolic Extracts of Duguetia vallicola (Annonaceae) against in-Hospital Isolates of Pseudomonas aeruginosa
by Orfa Inés Contreras-Martínez, Daniela Sierra-Quiroz and Alberto Angulo-Ortíz
Plants 2024, 13(10), 1412; https://doi.org/10.3390/plants13101412 (registering DOI) - 18 May 2024
Abstract
Pseudomonas aeruginosa is an opportunistic pathogen that is especially dominant in people with cystic fibrosis; the drug resistance expressed by this pathogen and its capacity for adaptation poses a significant challenge to its treatment and control, thereby increasing morbidity and mortality rates globally. [...] Read more.
Pseudomonas aeruginosa is an opportunistic pathogen that is especially dominant in people with cystic fibrosis; the drug resistance expressed by this pathogen and its capacity for adaptation poses a significant challenge to its treatment and control, thereby increasing morbidity and mortality rates globally. In this sense, the search for new treatment alternatives is imminent today, with products of plant origin being an excellent alternative for use. The objective of this research was to evaluate the antibacterial and antibiofilm potential and to explore the possible effect of ethanolic extracts from the wood and bark of Duguetia vallicola on the cell membrane. Microdilution assays showed the inhibition of bacterial growth by more than 50%, with the lowest concentration (62.5 μg/mL) of both extracts evaluated. Furthermore, we report the ability of both extracts to inhibit mature biofilms, with inhibition percentages between 48.4% and 93.7%. Intracellular material leakage experiments (260/280 nm), extracellular pH measurements, and fluorescence microscopy with acridine orange (AO) and ethidium bromide (EB) showed cell membrane damage. This indicates that the antibacterial action of ethanolic extracts of D. vallicola is associated with damage to the integrity of the cell membrane and consequent death of these pathogens. These results serve as a reference for future studies in establishing the mechanisms of action of these extracts. Full article
(This article belongs to the Special Issue Biological Activities of Plant Extracts 2023)
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18 pages, 10868 KiB  
Article
A Well-Established Gut Microbiota Enhances the Efficiency of Nutrient Metabolism and Improves the Growth Performance of Trachinotus ovatus
by Miao Kong, Wendong Zhao, Cong Wang, Jie Qi, Jinxiang Liu and Quanqi Zhang
Int. J. Mol. Sci. 2024, 25(10), 5525; https://doi.org/10.3390/ijms25105525 (registering DOI) - 18 May 2024
Abstract
The gut microbiota has become an essential component of the host organism and plays a crucial role in the host immune system, metabolism, and physiology. Nevertheless, our comprehension of how the fish gut microbiota contributes to enhancing nutrient utilization in the diet and [...] Read more.
The gut microbiota has become an essential component of the host organism and plays a crucial role in the host immune system, metabolism, and physiology. Nevertheless, our comprehension of how the fish gut microbiota contributes to enhancing nutrient utilization in the diet and improving host growth performance remains unclear. In this study, we employed a comprehensive analysis of the microbiome, metabolome, and transcriptome to analyze intestines of the normal control group and the antibiotic-treated model group of T. ovatus to investigate how the gut microbiota enhances fish growth performance and uncover the underlying mechanisms. First, we found that the growth performance of the control group was significantly higher than that of the antibiotic-treated model under the same feeding conditions. Subsequent multiomics analyses showed that the gut microbiota can improve its own composition by mediating the colonization of some probiotics represented by Lactobacillus in the intestine, improving host metabolic efficiency with proteins and lipids, and also influencing the expression of genes in signaling pathways related to cell proliferation, which together contribute to the improved growth performance of T. ovatus. Our results demonstrated the important contribution of gut microbiota and its underlying molecular mechanisms on the growth performance of T. ovatus. Full article
(This article belongs to the Special Issue Microbial Omics)
15 pages, 2271 KiB  
Article
Pushing the Limit of Photo-Controlled Polymerization: Hyperchromic and Bathochromic Effects
by Zhilei Wang, Zipeng Zhang, Chenyu Wu, Zikuan Wang and Wenjian Liu
Molecules 2024, 29(10), 2377; https://doi.org/10.3390/molecules29102377 (registering DOI) - 18 May 2024
Abstract
The photocatalyst (PC) zinc tetraphenylporphyrin (ZnTPP) is highly efficient for photoinduced electron/energy transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. However, ZnTPP suffers from poor absorbance of orange light by the so-called Q-band of the absorption spectrum (maximum absorption wavelength λmax = 600 [...] Read more.
The photocatalyst (PC) zinc tetraphenylporphyrin (ZnTPP) is highly efficient for photoinduced electron/energy transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. However, ZnTPP suffers from poor absorbance of orange light by the so-called Q-band of the absorption spectrum (maximum absorption wavelength λmax = 600 nm, at which molar extinction coefficient εmax = 1.0×104 L/(mol·cm)), hindering photo-curing applications that entail long light penetration paths. Over the past decade, there has not been any competing candidate in terms of efficiency, despite a myriad of efforts in PC design. By theoretical evaluation, here we rationally introduce a peripheral benzo moiety on each of the pyrrole rings of ZnTPP, giving zinc tetraphenyl tetrabenzoporphyrin (ZnTPTBP). This modification not only enlarges the conjugation length of the system, but also alters the a1u occupied π molecular orbital energy level and breaks the accidental degeneracy between the a1u and a2u orbitals, which is responsible for the low absorption intensity of the Q-band. As a consequence, not only is there a pronounced hyperchromic and bathochromic effect (λmax = 655 nm and εmax = 5.2×104 L/(mol·cm)) of the Q-band, but the hyperchromic effect is achieved without increasing the intensity of the less useful, low wavelength absorption peaks of the PC. Remarkably, this strong 655 nm absorption takes advantage of deep-red (650–700 nm) light, a major component of solar light exhibiting good atmosphere penetration, exploited by the natural PC chlorophyll a as well. Compared with ZnTPP, ZnTPTBP displayed a 49% increase in PET-RAFT polymerization rate with good control, marking a significant leap in the area of photo-controlled polymerization. Full article
(This article belongs to the Special Issue Themed Issue Dedicated to Prof. Bernard Boutevin)
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31 pages, 1604 KiB  
Article
Respiratory Condition Detection Using Audio Analysis and Convolutional Neural Networks Optimized by Modified Metaheuristics
by Nebojsa Bacanin, Luka Jovanovic, Ruxandra Stoean, Catalin Stoean, Miodrag Zivkovic, Milos Antonijevic and Milos Dobrojevic
Axioms 2024, 13(5), 335; https://doi.org/10.3390/axioms13050335 (registering DOI) - 18 May 2024
Abstract
Respiratory conditions have been a focal point in recent medical studies. Early detection and timely treatment are crucial factors in improving patient outcomes for any medical condition. Traditionally, doctors diagnose respiratory conditions through an investigation process that involves listening to the patient’s lungs. [...] Read more.
Respiratory conditions have been a focal point in recent medical studies. Early detection and timely treatment are crucial factors in improving patient outcomes for any medical condition. Traditionally, doctors diagnose respiratory conditions through an investigation process that involves listening to the patient’s lungs. This study explores the potential of combining audio analysis with convolutional neural networks to detect respiratory conditions in patients. Given the significant impact of proper hyperparameter selection on network performance, contemporary optimizers are employed to enhance efficiency. Moreover, a modified algorithm is introduced that is tailored to the specific demands of this study. The proposed approach is validated using a real-world medical dataset and has demonstrated promising results. Two experiments are conducted: the first tasked models with respiratory condition detection when observing mel spectrograms of patients’ breathing patterns, while the second experiment considered the same data format for multiclass classification. Contemporary optimizers are employed to optimize the architecture selection and training parameters of models in both cases. Under identical test conditions, the best models are optimized by the introduced modified metaheuristic, with an accuracy of 0.93 demonstrated for condition detection, and a slightly reduced accuracy of 0.75 for specific condition identification. Full article
(This article belongs to the Special Issue Advances in Parameter-Tuning Techniques for Metaheuristic Algorithms)
21 pages, 345 KiB  
Article
The Operational Risk Disclosure Threshold Effect in the Earnings Management–Sustainability Firm Performance Nexus in Saudi Arabia: A Dynamic Panel Threshold Regression Model
by Faizah Alsulami
Sustainability 2024, 16(10), 4264; https://doi.org/10.3390/su16104264 (registering DOI) - 18 May 2024
Abstract
Although the relationship between earnings management and firm performance has been well explored in the literature, sustainable performance has not yet been examined. Furthermore, the literature has not addressed the issue of nonlinearity between earnings management and firm performance. Therefore, this paper aims [...] Read more.
Although the relationship between earnings management and firm performance has been well explored in the literature, sustainable performance has not yet been examined. Furthermore, the literature has not addressed the issue of nonlinearity between earnings management and firm performance. Therefore, this paper aims to examine the potential nonlinear relationship between earnings management and sustainable firm performance in Saudi Arabia using a sample of 70 listed firms over the 2015–2022 period. Specifically, it investigates the operational risk disclosure threshold effect in the earning management–sustainable firm performance nexus. To do so, the dynamic panel threshold regression model (DPTR) is performed. The result proves that there is a threshold effect of operational risk disclosure in the relationship between earning management and sustainable firm performance. Specifically, the threshold values of operational risk disclosure for the three models are estimated at 6 between the low- and the high-operational-risk-disclosure regimes. In the lower regime, firm performance decreases when earning management increases; however, in the higher regime, firm performance increases when earning management increases. These outcomes support the predictions of agency and positive accounting theories. Full article
34 pages, 2951 KiB  
Perspective
Retinoid Synthesis Regulation by Retinal Cells in Health and Disease
by Massimiliano Andreazzoli, Biancamaria Longoni, Debora Angeloni and Gian Carlo Demontis
Cells 2024, 13(10), 871; https://doi.org/10.3390/cells13100871 (registering DOI) - 18 May 2024
Abstract
Vision starts in retinal photoreceptors when specialized proteins (opsins) sense photons via their covalently bonded vitamin A derivative 11cis retinaldehyde (11cis-RAL). The reaction of non-enzymatic aldehydes with amino groups lacks specificity, and the reaction products may trigger cell damage. However, the reduced synthesis [...] Read more.
Vision starts in retinal photoreceptors when specialized proteins (opsins) sense photons via their covalently bonded vitamin A derivative 11cis retinaldehyde (11cis-RAL). The reaction of non-enzymatic aldehydes with amino groups lacks specificity, and the reaction products may trigger cell damage. However, the reduced synthesis of 11cis-RAL results in photoreceptor demise and suggests the need for careful control over 11cis-RAL handling by retinal cells. This perspective focuses on retinoid(s) synthesis, their control in the adult retina, and their role during retina development. It also explores the potential importance of 9cis vitamin A derivatives in regulating retinoid synthesis and their impact on photoreceptor development and survival. Additionally, recent advancements suggesting the pivotal nature of retinoid synthesis regulation for cone cell viability are discussed. Full article
(This article belongs to the Section Cell Signaling)
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24 pages, 545 KiB  
Article
Neural Architecture Comparison for Bibliographic Reference Segmentation: An Empirical Study
by Rodrigo Cuéllar Hidalgo, Raúl Pinto Elías, Juan Manuel Torres Moreno, Osslan Osiris Vergara Villegas , Gerardo Reyes Salgado and Andrea Magadán Salazar
Data 2024, 9(5), 71; https://doi.org/10.3390/data9050071 (registering DOI) - 18 May 2024
Abstract
In the realm of digital libraries, efficiently managing and accessing scientific publications necessitates automated bibliographic reference segmentation. This study addresses the challenge of accurately segmenting bibliographic references, a task complicated by the varied formats and styles of references. Focusing on the empirical evaluation [...] Read more.
In the realm of digital libraries, efficiently managing and accessing scientific publications necessitates automated bibliographic reference segmentation. This study addresses the challenge of accurately segmenting bibliographic references, a task complicated by the varied formats and styles of references. Focusing on the empirical evaluation of Conditional Random Fields (CRF), Bidirectional Long Short-Term Memory with CRF (BiLSTM + CRF), and Transformer Encoder with CRF (Transformer + CRF) architectures, this research employs Byte Pair Encoding and Character Embeddings for vector representation. The models underwent training on the extensive Giant corpus and subsequent evaluation on the Cora Corpus to ensure a balanced and rigorous comparison, maintaining uniformity across embedding layers, normalization techniques, and Dropout strategies. Results indicate that the BiLSTM + CRF architecture outperforms its counterparts by adeptly handling the syntactic structures prevalent in bibliographic data, achieving an F1-Score of 0.96. This outcome highlights the necessity of aligning model architecture with the specific syntactic demands of bibliographic reference segmentation tasks. Consequently, the study establishes the BiLSTM + CRF model as a superior approach within the current state-of-the-art, offering a robust solution for the challenges faced in digital library management and scholarly communication. Full article
26 pages, 5436 KiB  
Article
High-Caloric Diets in Adolescence Impair Specific GABAergic Subpopulations, Neurogenesis, and Alter Astrocyte Morphology
by Bárbara Mota, Ana Rita Brás, Leonardo Araújo-Andrade, Ana Silva, Pedro A. Pereira, M. Dulce Madeira and Armando Cardoso
Int. J. Mol. Sci. 2024, 25(10), 5524; https://doi.org/10.3390/ijms25105524 (registering DOI) - 18 May 2024
Abstract
We compared the effects of two different high-caloric diets administered to 4-week-old rats for 12 weeks: a diet rich in sugar (30% sucrose) and a cafeteria diet rich in sugar and high-fat foods. We focused on the hippocampus, particularly on the gamma-aminobutyric acid [...] Read more.
We compared the effects of two different high-caloric diets administered to 4-week-old rats for 12 weeks: a diet rich in sugar (30% sucrose) and a cafeteria diet rich in sugar and high-fat foods. We focused on the hippocampus, particularly on the gamma-aminobutyric acid (GABA)ergic system, including the Ca2+-binding proteins parvalbumin (PV), calretinin (CR), calbindin (CB), and the neuropeptides somatostatin (SST) and neuropeptide Y (NPY). We also analyzed the density of cholinergic varicosities, brain-derived neurotrophic factor (BDNF), reelin (RELN), and cyclin-dependent kinase-5 (CDK-5) mRNA levels, and glial fibrillary acidic protein (GFAP) expression. The cafeteria diet reduced PV-positive neurons in the granular layer, hilus, and CA1, as well as NPY-positive neurons in the hilus, without altering other GABAergic populations or overall GABA levels. The high-sugar diet induced a decrease in the number of PV-positive cells in CA3 and an increase in CB-positive cells in the hilus and CA1. No alterations were observed in the cholinergic varicosities. The cafeteria diet also reduced the relative mRNA expression of RELN without significant changes in BDNF and CDK5 levels. The cafeteria diet increased the number but reduced the length of the astrocyte processes. These data highlight the significance of determining the mechanisms mediating the observed effects of these diets and imply that the cognitive impairments previously found might be related to both the neuroinflammation process and the reduction in PV, NPY, and RELN expression in the hippocampal formation. Full article
(This article belongs to the Special Issue Focus on Hippocampus Biology: From Neurophysiology to Dysfunctions)
14 pages, 3904 KiB  
Article
Isolation, Characterization, and Pathogenicity of an Aeromonas veronii Strain Causing Disease in Rhinogobio ventralis
by Xingbing Wu, Baolin Cheng, Mingyang Xue, Nan Jiang, Xuemei Li, Xiaona Hu, Xiaoli Li, Tingbing Zhu, Yongjiu Zhu and Yong Zhou
Fishes 2024, 9(5), 188; https://doi.org/10.3390/fishes9050188 (registering DOI) - 18 May 2024
Abstract
Rhinogobio ventralis is a rare fish found in the Yangtze River in China and has significant ecological and economic value. In this study, a bacterial strain (RV-JZ01) was isolated from the livers of diseased R. ventralis. This isolate was identified as Aeromonas [...] Read more.
Rhinogobio ventralis is a rare fish found in the Yangtze River in China and has significant ecological and economic value. In this study, a bacterial strain (RV-JZ01) was isolated from the livers of diseased R. ventralis. This isolate was identified as Aeromonas veronii based on its morphology, biochemical features and 16S rDNA phylogenetic analysis. The artificial infection of healthy R. ventralis (16 ± 2 cm) with RV-JZ01 resulted in the manifestation of clinical symptoms, in accordance with those of naturally infected fish. The 50% lethal dose (LD50) of RV-JZ01 for R. ventralis was 6.3 × 106 CFU/mL. Histopathological examination revealed various pathological changes in the diseased fish, including intestinal villus swelling and rupture, hepatocyte vacuolization, renal tubular cell nuclear enlargement and pyknosis, and myocardial fiber fracture and atrophy. RV-JZ01 infection significantly reduced the gut flora diversity of R. ventralis, with the relative abundances of Firmicutes and Fusobacteria increasing, and those of the Proteobacteria and Bacteroidetes decreasing. The abundance of Lactobacillus and Streptococcus dramatically increased, and the abundance of Clostridium and Escherichia reduced in the intestinal microbiota of R. ventralis infected with RV-JZ01. Antibiotic sensitivity testing revealed that RV-JZ01 was highly susceptible to 12 antimicrobials, including erythromycin, cefalexin, norfloxacin, furazolidone, sulfonamides, enrofloxacin, doxycycline, piperacillin, florfenicol, gentamicin, and lincomycin. These results contribute to the understanding of pathological alterations in R. ventralis following A. veronii infection, offering valuable data to support the implementation of disease treatment. Full article
(This article belongs to the Special Issue Fish Diseases Diagnostics and Prevention in Aquaculture)
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15 pages, 2267 KiB  
Article
Exacerbated Activation of the NLRP3 Inflammasome in the Placentas from Women Who Developed Chronic Venous Disease during Pregnancy
by María Asunción Sánchez-Gil, Oscar Fraile-Martinez, Cielo García-Montero, Diego De Leon-Oliva, Diego Liviu Boaru, Patricia De Castro-Martinez, Adrían Camacho-Alcázar, Juan A. De León-Luis, Coral Bravo, Raúl Díaz-Pedrero, Laura López-Gonzalez, Julia Bujan, María J. Cancelo, Melchor Álvarez-Mon, Natalio García-Honduvilla, Miguel A. Saez and Miguel A. Ortega
Int. J. Mol. Sci. 2024, 25(10), 5528; https://doi.org/10.3390/ijms25105528 (registering DOI) - 18 May 2024
Abstract
Chronic venous disease (CVD) comprises a spectrum of morphofunctional disorders affecting the venous system, affecting approximately 1 in 3 women during gestation. Emerging evidence highlights diverse maternofetal implications stemming from CVD, particularly impacting the placenta. While systemic inflammation has been associated with pregnancy-related [...] Read more.
Chronic venous disease (CVD) comprises a spectrum of morphofunctional disorders affecting the venous system, affecting approximately 1 in 3 women during gestation. Emerging evidence highlights diverse maternofetal implications stemming from CVD, particularly impacting the placenta. While systemic inflammation has been associated with pregnancy-related CVD, preliminary findings suggest a potential link between this condition and exacerbated inflammation in the placental tissue. Inflammasomes are major orchestrators of immune responses and inflammation in different organs and systems. Notwithstanding the relevance of inflammasomes, specifically the NLRP3 (nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3)- which has been demonstrated in the placentas of women with different obstetric complications, the precise involvement of this component in the placentas of women with CVD remains to be explored. This study employs immunohistochemistry and real-time PCR (RT-qPCR) to examine the gene and protein expression of key components in both canonical and non-canonical pathways of the NLRP3 inflammasome (NLRP3, ASC—apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain—caspase 1, caspase 5, caspase 8, and interleukin 1β) within the placental tissue of women affected by CVD. Our findings reveal a substantial upregulation of these components in CVD-affected placentas, indicating a potential pathophysiological role of the NLRP3 inflammasome in the development of this condition. Subsequent investigations should focus on assessing translational interventions addressing this dysregulation in affected patient populations. Full article
28 pages, 5155 KiB  
Article
Re-Inventories at Two Study Sites in the Oberpfalz (Bavaria, Germany): Increase in RWA Nests, Herb Biodiversity and Dead Wood in Sustainably Managed Forests
by Gabriele M. Berberich and Martin B. Berberich
Sustainability 2024, 16(10), 4265; https://doi.org/10.3390/su16104265 (registering DOI) - 18 May 2024
Abstract
A large-scale re-inventory of red wood ant (RWA; Formica rufa-group) nests and a comparative analysis of their presence/absence data was carried out for the first time in 2023 at two study sites in the Oberpfalz, NE Bavaria, Germany, to investigate the suspected [...] Read more.
A large-scale re-inventory of red wood ant (RWA; Formica rufa-group) nests and a comparative analysis of their presence/absence data was carried out for the first time in 2023 at two study sites in the Oberpfalz, NE Bavaria, Germany, to investigate the suspected decline of nests, their relationship with forestry aspects, and possible changes in spatial distribution due to tectonic activity (GeoBio-Interactions). We inventoried, in two sustainably managed forests, outstanding nest occurrences (5393 nests including 5276 active nests) and an increase of ≈8% over 4–7 years. Our results do not support claims that the number of nests in the Oberpfalz is in sharp decline. RWA preferred mature and medium–mature pine-dominated forests. Standing and downed dead wood did not negatively affect nests. The number of nests with woodpecker cavities and the diversity of the herb layer increased. Nests clusters in NW–SE direction suggest a strong interaction between nest distribution and active tectonics. Our (re-)inventory approach combined with the extensive photo database is a valuable tool for (a) monitoring the entire forest habitat including natural restocking, herb biodiversity, woodpecker cavities, and dead wood in, at, and around nests, and (b) identifying GeoBio-Interactions. Such an approach will lead to more realistic counts of RWA nests and provide scientific evidence of the current situation of nests occurrence. We suggest seven to eight years as an appropriate time interval for re-inventories. Full article
(This article belongs to the Section Sustainable Forestry)
18 pages, 4034 KiB  
Article
A Methodology for Post-Necking Analysis in Isotropic Metals
by Martina Scapin and Marta Beltramo
Metals 2024, 14(5), 593; https://doi.org/10.3390/met14050593 (registering DOI) - 18 May 2024
Abstract
Metallic materials are commonly characterized through tensile tests. For ductile metals, a consistent part of the test occurs after the necking onset. A first estimate of the post-necking behavior could be obtained by extrapolating the mathematical model that fits the pre-necking law. However, [...] Read more.
Metallic materials are commonly characterized through tensile tests. For ductile metals, a consistent part of the test occurs after the necking onset. A first estimate of the post-necking behavior could be obtained by extrapolating the mathematical model that fits the pre-necking law. However, as well known, the accuracy of the predictions would not be guaranteed. Therefore, over the past decades many efforts have been devoted to dealing with the necking phenomenon. The most popular correction formula proposed by Bridgman is an analytical method based on the neck geometry. Despite being widely used, it may not be accurate at large strains due to the assumption of uniform distribution of the equivalent stress and equivalent strain in the specimen minimum cross-section. Starting from Bridgman’s idea and in order to overcome its limitations, the present paper develops an efficient method to calibrate the hardening law of isotropic metallic materials at large strains. The proposed method requires to record the outer contour of the necking zone during the test and to build a dataset of necking deformed shapes. Experimental quasi-static tensile tests were analyzed with the proposed approach, which appears promising when critically compared with other methods. Full article
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11 pages, 1692 KiB  
Article
3-Hydroxyanthranilic Acid Delays Paralysis in Caenorhabditis elegans Models of Amyloid-Beta and Polyglutamine Proteotoxicity
by Bradford T. Hull, Kayla M. Miller, Caroline Corban, Grant Backer, Susan Sheehan, Ron Korstanje and George L. Sutphin
Biomolecules 2024, 14(5), 599; https://doi.org/10.3390/biom14050599 (registering DOI) - 18 May 2024
Abstract
Age is the primary risk factor for neurodegenerative diseases such as Alzheimer’s and Huntington’s disease. Alzheimer’s disease is the most common form of dementia and a leading cause of death in the elderly population of the United States. No effective treatments for these [...] Read more.
Age is the primary risk factor for neurodegenerative diseases such as Alzheimer’s and Huntington’s disease. Alzheimer’s disease is the most common form of dementia and a leading cause of death in the elderly population of the United States. No effective treatments for these diseases currently exist. Identifying effective treatments for Alzheimer’s, Huntington’s, and other neurodegenerative diseases is a major current focus of national scientific resources, and there is a critical need for novel therapeutic strategies. Here, we investigate the potential for targeting the kynurenine pathway metabolite 3-hydroxyanthranilic acid (3HAA) using Caenorhabditis elegans expressing amyloid-beta or a polyglutamine peptide in body wall muscle, modeling the proteotoxicity in Alzheimer’s and Huntington’s disease, respectively. We show that knocking down the enzyme that degrades 3HAA, 3HAA dioxygenase (HAAO), delays the age-associated paralysis in both models. This effect on paralysis was independent of the protein aggregation in the polyglutamine model. We also show that the mechanism of protection against proteotoxicity from HAAO knockdown is mimicked by 3HAA supplementation, supporting elevated 3HAA as the mediating event linking HAAO knockdown to delayed paralysis. This work demonstrates the potential for 3HAA as a targeted therapeutic in neurodegenerative disease, though the mechanism is yet to be explored. Full article
(This article belongs to the Special Issue Molecular Advances in Mechanism and Regulation of Lifespan and Aging)
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15 pages, 15048 KiB  
Article
Pollen and Floral Organ Morphology of 18 Oil-Tea Genotypes and Its Systematic Significance
by Qian Yin, Zhongfei Pan, Yanming Li, Huan Xiong, Joseph Masabni, Deyi Yuan and Feng Zou
Horticulturae 2024, 10(5), 524; https://doi.org/10.3390/horticulturae10050524 (registering DOI) - 18 May 2024
Abstract
Oil-tea belongs to the Camellia genus, an important oil crop in China. However, oil-tea is taxonomically challenging due to its morphological variation, polyploidy, and interspecific hybridization. Therefore, the present study aimed to investigate the flower organs’ morphology and pollen micro-morphology of 18 oil-tea [...] Read more.
Oil-tea belongs to the Camellia genus, an important oil crop in China. However, oil-tea is taxonomically challenging due to its morphological variation, polyploidy, and interspecific hybridization. Therefore, the present study aimed to investigate the flower organs’ morphology and pollen micro-morphology of 18 oil-tea genotypes in detail and discussed their significance for oil-tea taxonomy. The quantitative parameters of flowers were measured using Vernier caliper measurements. Pollen morphology was observed and photographed using scanning electron microscopy (SEM). The results indicated that the flower size varied significantly among the tested oil-tea genotypes, with the corolla diameter ranging from 42.25 μm in C. meiocarpa ‘LP’ to 89.51 μm in C. oleifera ‘ASX09’. The pollen grains of oil-tea are monads and medium grade in pollen size. There were two types of polar views, including triangular or subcircular, with a polar axis length (P) ranging from 27.5 μm in C. oleifera ‘CY67’ to 59.04 μm in C. mairei (H. Lév.) Melch. var. lapidea (Y.C. Wu) Sealy. The equatorial views exhibited oblate, spherical, or oblong shapes, with an equatorial axis length (E) of 21.32 to 41.62 μm. The pollen exine sculpture was perforate, verrucate, and reticulate. The perforation lumina diameter (D) ranged from 0.29 μm in C. magniflora Chang to 1.22 μm in C. yuhsienensis Hu, and the perforation width (W) varied from 0.77 μm in C. osmantha to 1.40 μm in C. gauchowensis ‘HM349’, respectively. Qualitative clustering analysis (Q-type cluster) and principal component analysis (PCA) were conducted using eleven indexes of flower and pollen morphology, and the 18 oil-tea genotypes were classified into three categories. In addition, the correlation analysis showed that there was a significant correlation between pollen size and flower morphology or pollen exine sculpture. These results offer valuable information on the classification and identification of the 18 oil-tea germplasm resources. Full article
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10 pages, 3599 KiB  
Article
Temperature-Dependent Residual Stresses and Thermal Expansion Coefficient of VO2 Thin Films
by Chuen-Lin Tien, Chun-Yu Chiang, Ching-Chiun Wang and Shih-Chin Lin
Inventions 2024, 9(3), 61; https://doi.org/10.3390/inventions9030061 (registering DOI) - 18 May 2024
Abstract
This study aims to investigate the thermomechanical properties of vanadium dioxide (VO2) thin films. A VO2 thin film was simultaneously deposited on B270 and H-K9L glass substrates by electron-beam evaporation with ion-assisted deposition. Based on optical interferometric methods, the thermal–mechanical [...] Read more.
This study aims to investigate the thermomechanical properties of vanadium dioxide (VO2) thin films. A VO2 thin film was simultaneously deposited on B270 and H-K9L glass substrates by electron-beam evaporation with ion-assisted deposition. Based on optical interferometric methods, the thermal–mechanical behavior of and thermal stresses in VO2 films can be determined. An improved Twyman–Green interferometer was used to measure the temperature-dependent residual stress variations of VO2 thin films at different temperatures. This study found that the substrate has a great impact on thermal stress, which is mainly caused by the mismatch in the coefficient of thermal expansion (CTE) of the film and the substrate. By using the dual-substrate method, thermal stresses in VO2 thin films from room temperature to 120 °C can be evaluated. The thermal expansion coefficient is 3.21 × 10−5 °C−1, and the biaxial modulus is 517 GPa. Full article
(This article belongs to the Special Issue Thermodynamic and Technical Analysis for Sustainability (Volume 3))
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