KCTD1 plays crucial roles in regulating both the SHH and WNT/β-catenin signaling pathways, which are essential for tooth development. The objective of this study was to investigate if genetic variants in KCTD1 might also be associated with isolated dental anomalies. We clinically and radiographically investigated 362 patients affected with isolated dental anomalies. Whole exome sequencing identified two unrelated families with rare (p.Arg241Gln) or novel (p.Pro243Ser) variants in KCTD1. The variants segregated with the dental anomalies in all nine patients from the two families. Clinical findings of the patients included taurodontism, unseparated roots, long roots, tooth agenesis, a supernumerary tooth, torus palatinus, and torus mandibularis. The role of Kctd1 in root development is supported by our immunohistochemical study showing high expression of Kctd1 in Hertwig epithelial root sheath. The KCTD1 variants in our patients are the first variants found to be located in the C-terminal domain, which might disrupt protein–protein interactions and/or SUMOylation and subsequently result in aberrant WNT-SHH-BMP signaling and isolated dental anomalies. Functional studies on the p.Arg241Gln variant are consistent with an impact on β-catenin levels and canonical WNT signaling. This is the first report of the association of KCTD1 variants and isolated dental anomalies. Full article

Humans have influenced the sediment transport of rivers on a centurial scale. Our goal was to use the rate of lateral channel processes as a proxy to reconstruct sediment budget (SB) changes of a lowland river (Middle Tisza, Hungary) on a historical scale (1838–2017). The gross sediment budget (GSB) refers to the total area of eroded and accumulated surfaces, and the net sediment budget (NSB) indicates the sediment sink or source characteristics. At the beginning (1838–1890), the artificial cut-offs increased the slope and channel erosion, but the eroded sediment deposited in the oxbows, so the reach acted as a sediment sink (NSB: +0.1–0.8 m²/m/y). Then (1890–1929), a quasi-equilibrium state developed (NSB: −0.2 m²/m/y to +0.4 m²/m/y). Later (1929–1976), the bank protections impeded lateral erosion, so the system became a sediment sink again (NSB: +0.1–0.7 m²/m/y). Finally (1976–2017), the erosional processes accelerated due to dam construction and revetment collapses, and now the river is a sediment source (NSB: −0.03 to −0.08 m²/m/y). This study proved that (1) the actual SB could not be projected in the long term, as it was heavily modified, and (2) lateral channel changes could be used as a proxy to estimate long-term SB. Full article

Lysophosphatidic acid (LPA) species, prevalent in the tumor microenvironment (TME), adversely impact various cancers. In ovarian cancer, the 18:0 and 20:4 LPA species are selectively associated with shorter relapse-free survival, indicating distinct effects on cellular signaling networks. Macrophages represent a cell type of high relevance in the TME, but the impact of LPA on these cells remains obscure. Here, we uncovered distinct LPA-species-specific responses in human monocyte-derived macrophages through unbiased phosphoproteomics, with 87 and 161 phosphosites upregulated by 20:4 and 18:0 LPA, respectively, and only 24 shared sites. Specificity was even more pronounced for downregulated phosphosites (163 versus 5 sites). Considering the high levels 20:4 LPA in the TME and its selective association with poor survival, this finding may hold significant implications. Pathway analysis pinpointed RHO/RAC1 GTPase signaling as the predominantly impacted target, including AHRGEF and DOCK guanine exchange factors, ARHGAP GTPase activating proteins, and regulatory protein kinases. Consistent with these findings, exposure to 20:4 resulted in strong alterations to the actin filament network and a consequent enhancement of macrophage migration. Moreover, 20:4 LPA induced p38 phosphorylation, a response not mirrored by 18:0 LPA, whereas the pattern for AKT was reversed. Furthermore, RNA profiling identified genes involved in cholesterol/lipid metabolism as selective targets of 20:4 LPA. These findings imply that the two LPA species cooperatively regulate different pathways to support functions essential for pro-tumorigenic macrophages within the TME. These include cellular survival via AKT activation and migration through RHO/RAC1 and p38 signaling. Full article

In this paper, single-diode model (SDM) and double-diode model (DDM) parameters of the French RTC solar cell and the Photowatt PWP 201 photovoltaic (PV) module were extracted by combining five metaheuristic algorithms with three simulation current calculation methods (i.e., approximation method, Lambert W method and Newton–Raphson method), respectively. It was found that the parameter-extraction accuracies of the Lambert W (LW) method and the Newton–Raphson (NR) method are always approximately equal and higher than that of the approximation method. The best RMSEs (root mean square error) obtained by using the LW or the NR method on the solar cell and the PV module are 7.72986 × 10⁻⁴ and 2.05296 × 10⁻³ for SDM parameter extraction and 6.93709 × 10⁻⁴ and 1.99051 × 10⁻³ for DDM parameter extraction, respectively. The latter may be the highest parameter-extraction accuracy reported on the solar cell and the PV module so far, which is due to the adoption of more reasonable DDM parameter boundaries. Furthermore, the convergence curves of the LW and the NR method basically coincide, with a convergence speed faster than that of the approximation method. The robustness of a parameter-extraction method is mainly determined by the metaheuristic algorithm, but it is also affected by the simulation current calculation method and the parameter-extraction object. In a word, the approximation method is not suitable for application in PV-model parameter extraction because of incorrect estimation of the simulation current and the RMSE, while the LW and NR methods are suitable for the application for accurately calculating the simulation current and RMSE. In terms of saving computation resources and time, the NR method is superior to the LW method. Full article

The identification and characterization of immunogenic tumor antigens are essential for cancer vaccine development. In light of the impracticality of isolating and evaluating each putative antigen individually, in silico prediction algorithms, particularly those utilizing machine learning (ML) approaches, play a pivotal role. These algorithms significantly reduce the experimental workload necessary for discovering vaccine candidates. In this study, we employed six supervised ML methods on a dataset comprising 212 experimentally validated human tumor peptide antigens and an equal number of non-antigenic human peptides to develop models for immunogenicity prediction. These methods encompassed k-nearest neighbor (kNN), linear discriminant analysis (LDA), quadratic discriminant analysis (QDA), support vector machine (SVM), random forest (RF), and extreme gradient boosting (XGBoost). The models underwent validation through internal cross-validation within 10 groups from the training set and were further assessed using an external test set. Remarkably, the kNN model demonstrated superior performance, recognizing 90% of the known immunogens in the test set. The RF model excelled in the identification of non-immunogens, accurately classifying 93% of them in the test set. The three top-performing ML models according to multiple evaluation metrics (SVM, RF, and XGBoost) are to be subsequently integrated into the new version of the VaxiJen server, facilitating tumor antigen prediction through a majority voting mechanism. Full article

Photothermal techniques are infrared remote sensing techniques that have been used for biomedical applications, as well as industrial non-destructive testing (NDT). Machine learning is a branch of artificial intelligence, which includes a set of algorithms for learning from past data and analyzing new data, without being explicitly programmed to do so. In this paper, we first review the latest development of machine learning and its applications in photothermal techniques. Next, we present our latest work on machine learning for data analysis in opto-thermal transient emission radiometry (OTTER), which is a type of photothermal technique that has been extensively used in skin hydration, skin hydration depth profiles, skin pigments, as well as topically applied substances and skin penetration measurements. We have investigated different algorithms, such as random forest regression, gradient boosting regression, support vector machine (SVM) regression, and partial least squares regression, as well as deep learning neural network regression. We first introduce the theoretical background, then illustrate its applications with experimental results. Full article

The wine industry has been witnessing a growth in businesses crafting sustainability-certified wines and in the attention of consumers to sustainability, especially in the United States and Italy. To identify the characteristics of consumers who prefer sustainability-certified wine, this study analysed the relationship between consumers’ demographics, wine buying behaviour, and interest in sustainability-certified wine, focusing on these two countries for comparison. Data were collected through an online survey of US and Italian consumers. Through correspondence analysis, k-modes clustering analysis, and multi-way correspondence analysis, this study revealed a stronger relationship between demographics and interest in sustainability-certified wine among US consumers than Italian consumers. In particular, middle-aged US consumers exhibited a greater interest than seniors. The patterns of connections between consumers’ wine buying behaviour and interest in sustainable wine were similar for the two countries. In particular, consumers who purchase wine weekly had a keen interest, and those who purchase wine sporadically had no or little interest. Furthermore, this study uncovered the intricate relationship among various variables, providing a comprehensive understanding of the association between wine consumer characteristics and their interest in sustainability-certified wine. Full article

The advancement toward sustainable infrastructure presents complex multi-objective optimization (MOO) challenges. This paper expands the current understanding of design frameworks that balance cost, environmental impacts, social factors, and structural integrity. Integrating MOO with multi-criteria decision-making (MCDM), the study targets enhancements in life cycle sustainability for complex engineering projects using precast modular road frames. Three advanced evolutionary algorithms—NSGA-II, NSGA-III, and RVEA—are optimized and deployed to address sustainability objectives under performance constraints. The efficacy of these algorithms is gauged through a comparative analysis, and a robust MCDM approach is applied to nine non-dominated solutions, employing SAW, FUCA, TOPSIS, PROMETHEE, and VIKOR decision-making techniques. An entropy theory-based method ensures systematic, unbiased criteria weighting, augmenting the framework’s capacity to pinpoint designs balancing life cycle sustainability. The results reveal that NSGA-III is the algorithm converging towards the most cost-effective solutions, surpassing NSGA-II and RVEA by 21.11% and 10.07%, respectively, while maintaining balanced environmental and social impacts. The RVEA achieves up to 15.94% greater environmental efficiency than its counterparts. The analysis of non-dominated solutions identifies the $A_4$ design, utilizing 35 MPa concrete and B500S steel, as the most sustainable alternative across 80% of decision-making algorithms. The ranking correlation coefficients above 0.94 demonstrate consistency among decision-making techniques, underscoring the robustness of the integrated MOO and MCDM framework. The results in this paper expand the understanding of the applicability of novel techniques for enhancing engineering practices and advocate for a comprehensive strategy that employs advanced MOO algorithms and MCDM to enhance sustainable infrastructure development. Full article

The results of an experimental and mathematical study into the MmNi_4.2Mn_0.8 compound’s hydrogen storage properties are presented in the present research. Plotting and discussion of the experimental isotherms (P-C-T) for different starting temperatures (288 K, 298 K, 308 K, and 318 K) were carried out first. Then, the enthalpy and entropy of formation (ΔH₀, ΔS₀) were deduced from the plot of van’t Hoff. Following that, the P-C-T were contrasted with a mathematical model developed via statistical physics modeling. The steric and energetic parameters, such as the number of the receiving sites (n₁, n₂), their densities (N_m1, N_m2), and the energy parameters (P₁, P₂) of the system, were calculated thanks to the excellent agreement between the numerical and experimental results. Therefore, plotting and discussing these parameters in relation to temperature preceded their application in determining the amount of hydrogen in each type of site per unit of metal ([H/M]₁, [H/M]₂) as well as for the entire system [H/M] versus temperature and pressure besides the absorption energies associated with each kind of site (ΔE¹, ΔE²) and the thermodynamic functions (free energy, Gibbs energy, and entropy) that control the absorption reaction. Full article

This paper discusses thermal infrared (TIR) remote sensing technology applied to the delineation of geothermal resources, a significant renewable energy source. The technical characteristics and current status of TIR remote sensing is discussed and related to the integration of geological structure, geophysical data, and geochemical analyses. Also discussed are surface temperature inversion algorithms used to delineate anomalous ground-surface temperatures. Unlike traditional geophysical and geochemical exploration methods, remote sensing technology exhibits considerable advantages in terms of convenience and coverage extent. The paper addresses the major challenges and issues associated with using TIR remote sensing technology in geothermal prospecting. Full article

One of the hallmarks of cancer is metabolic reprogramming in tumor cells, and aerobic glycolysis is the primary mechanism by which glucose is quickly transformed into lactate. As one of the primary rate-limiting enzymes, pyruvate kinase (PK) M is engaged in the last phase of aerobic glycolysis. Alternative splicing is a crucial mechanism for protein diversity, and it promotes PKM precursor mRNA splicing to produce PKM2 dominance, resulting in low PKM1 expression. Specific splicing isoforms are produced in various tissues or illness situations, and the post-translational modifications are linked to numerous disorders, including cancers. hnRNPs are one of the main components of the splicing factor families. However, there have been no comprehensive studies on hnRNPs regulating PKM alternative splicing. Therefore, this review focuses on the regulatory network of hnRNPs on PKM pre-mRNA alternative splicing in tumors and clinical drug research. We elucidate the role of alternative splicing in tumor progression, prognosis, and the potential mechanism of abnormal RNA splicing. We also summarize the drug targets retarding tumorous splicing events, which may be critical to improving the specificity and effectiveness of current therapeutic interventions. Full article

It is widely known that all-female fish production holds economic value for aquaculture. Sebastes schlegelii, a preeminent economic species, exhibits a sex dimorphism, with females surpassing males in growth. In this regard, achieving all-female black rockfish production could significantly enhance breeding profitability. In this study, we utilized the widely used male sex-regulating hormone, 17α-methyltestosterone (MT) at three different concentrations (20, 40, and 60 ppm), to produce pseudomales of S. schlegelii for subsequent all-female offspring breeding. Long-term MT administration severely inhibits the growth of S. schlegelii, while short term had no significant impact. Histological analysis confirmed sex reversal at all MT concentrations; however, both medium and higher MT concentrations impaired testis development. MT also influenced sex steroid hormone levels in pseudomales, suppressing E2 while increasing T and 11-KT levels. In addition, a transcriptome analysis revealed that MT down-regulated ovarian-related genes (cyp19a1a and foxl2) while up-regulating male-related genes (amh) in pseudomales. Furthermore, MT modulated the TGF-β signaling and steroid hormone biosynthesis pathways, indicating its crucial role in S. schlegelii sex differentiation. Therefore, the current study provides a method for achieving sexual reversal using MT in S. schlegelii and offers an initial insight into the underlying mechanism of sexual reversal in this species. Full article

Background and Objectives: Long-term outcomes of immediately postoperative rotational malreduction in the axial plane after operative treatment of supracondylar humeral fractures (SCHF) are unknown. This study aimed to investigate the long-term clinical outcomes and associated factors for immediately postoperative rotational malreduction of SCHF. Materials and methods: In this retrospective case–control study, 88 patients who underwent surgery for Gratland type III SCHF were enrolled between January 2012 and January 2020. Among them, 49 patients had immediately postoperative malrotational reduction (rotational malreduction group) and 39 patients had no rotational deformity (control group). To evaluate the associated factors for immediately postoperative rotational malreduction, demographic data, fracture patterns, physical examination signs, and preoperative radiological parameters were analyzed. To compare the clinical outcomes, operation time, range of motion of the elbow, time from operation to full range of motion, and Flynn criteria were evaluated. The Oxford elbow score was used to investigate long-term clinical outcomes for patients five years after operation. Results: The mean age was 5.7 ± 2.3 years and mean follow-up period was 15.7 ± 4.0 months. The rotational malreduction group had significantly more patients with oblique fracture pattern (p = 0.031) and Pucker sign (p = 0.016) and showed a significantly longer operative time (p = 0.029) than the control group. Although there was no significant difference in the range of elbow motion and the Flynn criteria, the Kaplan–Meier survival curve showed a longer time to recover the full range of elbow motion in the rotational malreduction group (p = 0.040). There were no significant differences in the long-term clinical outcomes assessed using the Oxford elbow score (p = 0.684). Conclusions: Oblique fracture pattern and Pucker sign may be associated with immediately postoperative rotational malreduction in the axial plane. Although patients with immediately postoperative rotational malreduction showed favorable results of long-term clinical outcomes, they required more weeks to recover the full range of elbow motion. Full article

Shale oil and gas wells usually experience a rapid decline in production due to their extremely low permeability and strong heterogeneity. As a crucial technique to harness potential and elevate extraction rates in aged wells (formations), refracturing is increasingly employed within oil and gas reservoirs globally. At present, the selection processes for refracturing, both of wells and layers, are somewhat subjective and necessitate considerable field data. However, the status of fracturing technology is difficult to control precisely, and the difference in construction effects is large. In this paper, well selection, formation selection, and the fracturing technology of shale oil and gas refracturing are deeply analyzed, and the technological status and main technical direction of refracturing technology at home and abroad are analyzed and summarized. The applicability, application potential, and main technical challenges of existing technology for different wells are discussed, combined with the field production dynamics. The results show that well and layer selection is the key to the successful application of refracturing technology, and the geological engineering parameters closely related to the remaining reservoir reserves and formation energy should be considered as the screening parameters. General temporary plugging refracturing technology has a low cost and a simple process, but it is difficult to accurately control the location of temporary plugging, and the construction effect is very different. Mechanical isolation refracturing technology permits the exact refurbishment of regions untouched by the initial fracturing. However, it is costly and complex in terms of construction. Consequently, cutting the costs of mechanical isolation refracturing technology stands as a pivotal research direction. Full article

The dermal–epidermal junction (DEJ) is essential for maintaining skin structural integrity and regulating cell survival and proliferation. Thus, DEJ rejuvenation is key for skin revitalization, particularly in age-related DEJ deterioration. Radiofrequency (RF) treatment, known for its ability to enhance collagen fiber production through thermal mechanisms and increase heat shock protein (HSP) expression, has emerged as a promising method for skin rejuvenation. Additionally, RF activates Piezo1, an ion channel implicated in macrophage polarization toward an M2 phenotype and enhanced TGF-β production. This study investigated the impact of RF treatment on HSP47 and HSP90 expression, known stimulators of DEJ protein expression. Furthermore, using in vitro and aged animal skin models, we assessed whether RF-induced Piezo1 activation and the subsequent M2 polarization could counter age-related DEJ changes. The RF treatment of H₂O₂-induced senescent keratinocytes upregulated the expression of HSP47, HSP90, TGF-β, and DEJ proteins, including collagen XVII. Similarly, the RF treatment of senescent macrophages increased Piezo1 and CD206 (M2 marker) expression. Conditioned media from RF-treated senescent macrophages enhanced the expression of TGF-β and DEJ proteins, such as nidogen and collagen IV, in senescent fibroblasts. In aged animal skin, RF treatment increased the expression of HSP47, HSP90, Piezo1, markers associated with M2 polarization, IL-10, and TGF-β. Additionally, RF treatment enhanced DEJ protein expression. Moreover, RF reduced lamina densa replication, disrupted lesions, promoted hemidesmosome formation, and increased epidermal thickness. Overall, RF treatment effectively enhanced DEJ protein expression and mitigated age-related DEJ structural changes by increasing HSP levels and activating Piezo1. Full article

This paper presents a new electric drive-reconfigured on-board charger and initial electromagnetic torque suppression method. This proposed reconfigured on-board charger does not need many components added to the original electric drive system: only a connector is needed, which is easy to add. Specifically, the inverter for propulsion is reconfigured as a buck chopper and a conduction path to match the reconfigured windings. Two of the machine phase windings serve as inductors, while the third phase winding is reutilized as a common-mode inductor. In addition, the initial charging torque is generated at the outset of the charging process, which may cause an instant shock or even rotational movement. In order to prevent vehicle movement, the reason for the charging torque and suppression method were analyzed. Further, predictive control of the model based on mutual inductance analysis was adopted, where the charging torque was directly used as a control object in the cost function. Finally, experimental performances were applied to verify the proposed reconfigured on-board charger under constant current and constant voltage charging. Full article

The unmanned combat system-of-systems (UCSoS) in modern warfare is comprised of various interconnected entities that work together to support mission accomplishment. The soaring number of entities makes the UCSoS fragile and susceptible to triggering cascading effects when exposed to uncertain disturbances such as attacks or failures. Reconfiguring the UCSoS to restore its effectiveness in a self-coordinated and adaptive manner based on the battlefield situation and operational requirements has attracted increasing attention. In this paper, we focus on the UCSoS reconstruction with heterogeneous costs, where the collaboration nodes may have different reconstruction costs. Specifically, we adopt the heterogeneous network to capture the interdependencies among combat entities and propose a more representative metric to evaluate the UCSoS reconstruction effectiveness. Next, we model the combat network reconstruction problem with heterogeneous costs as a nonlinear optimization problem and prove its NP-hardness. Then, we propose an approach called SoS-Restorer, which is based on deep reinforcement learning (DRL), to address the UCSoS reconstruction problem. The results show that SoS-Restorer can quickly generate reconstruction strategies and improve the operational capabilities of the UCSoS by about 20∼60% compared to the baseline algorithm. Furthermore, even when the size of the UCSoS exceeds that of the training data, SoS-Restorer exhibits robust generalization capability and can efficiently produce satisfactory results in real time. Full article

In this paper, a class of two-delay differential equations with coefficient-dependent delay is studied. The distribution of the roots of the eigenequation is discussed, and conditions for the stability of the internal equilibrium and the existence of Hopf bifurcation are obtained. Additionally, using the normal form method and the central manifold theory, the bifurcation direction and the stability for the periodic solution of Hopf bifurcation are calculated. Finally, the correctness of the theory is verified by numerical simulation. Full article

This paper focuses on mission planning and cooperative navigation algorithms for multi-drone systems aimed at LiDAR-based mapping. It aims at demonstrating how multi-UAV cooperation can be used to fulfill LiDAR data georeferencing accuracy requirements, as well as to improve data collection capabilities, e.g., increasing coverage per unit time and point cloud density. These goals are achieved by exploiting the CDGNSS/Vision paradigm and properly defining the formation geometry and the UAV trajectories. The paper provides analytical tools to estimate point density considering different types of scanning LIDAR and to define attitude/pointing requirements. These tools are then used to support centralized cooperation-aware mission planning aimed at complete coverage for different target geometries. The validity of the proposed framework is demonstrated through numerical simulations considering a formation of three vehicles tasked with a powerline inspection mission. The results show that cooperative navigation allows for the reduction of angular and positioning estimation uncertainties, which results in a georeferencing error reduction of an order of magnitude and equal to 16.7 cm in the considered case. Full article

This paper establishes a connection between control theory for partially observed discrete-event systems (DESs) and automated theorem proving (ATP) in the calculus of positively constructed formulas (PCFs). The language of PCFs is a complete first-order language providing a powerful tool for qualitative analysis of dynamical systems. Based on ATP in the PCF calculus, a new technique is suggested for checking observability as a property of formal languages, which is necessary for the existence of supervisory control of DESs. In the case of violation of observability, words causing a conflict can also be extracted with the help of a specially designed PCF. With an example of the problem of path planning by a robot in an unknown environment, we show the application of our approach at one of the levels of a robot control system. The prover Bootfrost developed to facilitate PCF refutation is also presented. The tests show positive results and perspectives for the presented approach. Full article

In the scheduling of hard real-time systems on multicore platforms, significant unpredictability arises from interference caused by shared hardware resources. The objective of this paper is to offer a schedulability analysis for such systems by assuming a general model that introduces interference as a time parameter for each task. The analysis assumes constrained deadlines and is provided for fixed priorities. It is based on worst-case response time analysis, which exists in the literature for monocore systems. We demonstrate that the worst-case response time is an upper bound, and we evaluate our proposal with synthetic loads and execution on a real platform. Full article

This study aimed to compare the effects of 12 weeks of functional strength training combined with aerobic training (TG) and traditional resistance training combined with aerobic training (CG) on the body composition, physical fitness, and movement quality of obese adolescents. Forty participants were randomly assigned to either the TG group (n = 20) or the CG group (n = 20). Each group underwent training five times per week, lasting 120 min each time, over a total period of 12 weeks. All participants followed a strict dietary program. Anthropometric parameters, body composition, physical fitness, and movement quality were evaluated at baseline and after intervention. A two-way repeated measures ANOVA observed a significant interaction between time and group for body mass (p = 0.043), body fat percentage (p = 0.045), body mass index (p = 0.025), neck circumference (p = 0.01), chest circumference (p = 0.027), left-hand grip strength (p = 0.043), right-hand grip strength (p = 0.048), standing broad jump (p = 0.044), and total Functional Movement Screen score (p = 0.003), and the improvement was greater for TG in comparison to CG. TG was found to be more effective than CG in enhancing body composition, physical fitness, and movement quality in obese adolescents. Full article