Completion and final examination comprise the final stages of a doctoral program and represent the culmination of the doctoral candidates’ years of research. In this entry, completion is defined as the writing and submission of a doctoral thesis, and final examination is defined as the viva voce. Over the years, the format and scope of doctoral degrees has expanded and a variety of formats are now offered. In addition to the traditional research-only doctoral degree, professional, practice-based, and new route programs also contain a taught element alongside research. However, the creation of a substantive thesis or practice-based alternative addressing a novel research question is common to all. In contrast, processes and formats of viva voces vary across the globe. These range from private, closed-door defenses to assessed or ritualistic public defense presentations. For both completion and final examination, there are many practical and psychological hurdles that need to be navigated in order for the candidate to attain their doctoral degree. This entry will highlight these aspects as well as provide evidence-based guidance for supervisors in supporting their doctoral candidates through these daunting final stages. Full article

Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most widely utilized pharmaceuticals worldwide. Besides their recognized anti-inflammatory effects, these drugs exhibit various other pleiotropic effects in several cells, including platelets. Within this article, the multifaceted properties of NSAIDs on platelet functions, activation and viability, as well as their interaction(s) with established antiplatelet medications, by hindering several platelet agonists’ pathways and receptors, are thoroughly reviewed. The efficacy and safety of NSAIDs as adjunctive therapies for conditions involving inflammation and platelet activation are also discussed. Emphasis is given to the antiplatelet potential of commonly administered NSAIDs medications, such as ibuprofen, diclofenac, naproxen and ketoprofen, alongside non-opioid analgesic and antipyretic medications like paracetamol. This article delves into their mechanisms of action against different pathways of platelet activation, aggregation and overall platelet functions, highlighting additional health-promoting properties of these anti-inflammatory and analgesic agents, without neglecting the induced by these drugs’ side-effects on platelets’ functionality and thrombocytopenia. Environmental issues emerging from the ever-increased subscription of these drugs are also discussed, along with the need for novel water treatment methodologies for their appropriate elimination from water and wastewater samples. Despite being efficiently eliminated during wastewater treatment processes on occasion, NSAIDs remain prevalent and are found at significant concentrations in water bodies that receive effluents from wastewater treatment plants (WWTPs), since there is no one-size-fits-all solution for removing all contaminants from wastewater, depending on the specific characteristics of the wastewater. Several novel methods have been studied, with adsorption being proposed as a cost-effective and environmentally friendly method for wastewater purification from such drugs. This article also presents limitations and future prospects regarding the observed antiplatelet effects of NSAIDs, as well as the potential of novel derivatives of these compounds, with benefits in other important platelet functions. Full article

The proliferation of the Internet of Things (IoT) propels the continuous demand for compact, low-cost, and high-performance multiband filters. This paper introduces a novel low-profile dual-band bandpass filter (BPF) constructed with a back-to-back coupled pair of shielded folded quarter-mode substrate integrated waveguide (SF-QMSIW) multimode cavities. A hybrid structure is obtained by etching a coplanar waveguide (CPW) coupling line in the folded cavity’s septum layer. It serves multiple functions: generating an additional resonance, providing a separate coupling mechanism for the upper passband, and offering the flexibility to control the passbands’ center frequency ratio. Additionally, the unused second higher-order mode is suppressed by integrating embedded split-ring resonators (ESRRs) with an inter-digital capacitor (IDC) structure into the feed lines. A filter prototype has been fabricated and experimentally tested. The measurements confirmed reliable operation in two passbands having center frequencies 3.6 GHz and 5.8 GHz, and exhibiting 3 dB fractional bandwidths (FBWs) of 6.4% and 5.3%, respectively. Furthermore, the group delay variation within both passbands equals only 0.62 ns and 1.00 ns, respectively. Owing to the second higher-order mode suppression, the filter demonstrated an inter-band rejection exceeding 38 dB, within a compact footprint of 0.71${\lambda }_g^2$ (with ${\lambda }_g$ being the guided wavelength at the lower passband’s center frequency). Full article

Natural fiber composites have been extensively studied for structural applications, with recent exploration into their potential for various uses. This study investigates the impact of chemical treatments on the properties of Brazilian jute woven fabric/polyester resin composites. Sodium hydroxide, hydrogen peroxide, and peracetic acid were utilized to treat the jute fabrics, followed by resin transfer molding (RTM) to form the composites. Evaluation included water absorption, flexural strength, tensile strength, and short-beam strength. The alkaline treatment induced changes in the chemical composition of the fibers’ surface. Chemical treatments resulted in increased flexural and short-beam strength of the composites, with no significant alterations in tensile properties. The hydrogen peroxide treatment exhibited lower water absorption, suggesting its potential as a viable option for enhancing the performance of these composites. Full article

Coxiella burnetii is an obligate intracellular Gram-negative bacterium that causes Q fever, a life-threatening zoonotic disease. C. burnetii replicates within an acidified parasitophorous vacuole derived from the host lysosome. The ability of C. burnetii to replicate and achieve successful intracellular life in the cell cytosol is vastly dependent on the Dot/Icm type 4B secretion system (T4SSB). Although several T4SSB effector proteins have been shown to be important for C. burnetii virulence and intracellular replication, the role of the icmE protein in the host–C. burnetii interaction has not been investigated. In this study, we generated a C. burnetii Nine Mile Phase II (NMII) mutant library and identified 146 transposon mutants with a single transposon insertion. Transposon mutagenesis screening revealed that disruption of icmE gene resulted in the attenuation of C. burnetii NMII virulence in SCID mice. ELISA analysis indicated that the levels of pro-inflammatory cytokines, including interleukin-1β, IFN-γ, TNF-α, and IL-12p70, in serum from Tn::icmE mutant-infected SCID mice were significantly lower than those in serum from wild-type (WT) NMII-infected mice. Additionally, Tn::icmE mutant bacteria were unable to replicate in mouse bone marrow-derived macrophages (MBMDM) and human macrophage-like cells (THP-1). Immunoblotting results showed that the Tn::icmE mutant failed to activate inflammasome components such as IL-1β, caspase 1, and gasdermin-D in THP-1 macrophages. Collectively, these results suggest that the icmE protein may play a vital role in C. burnetii virulence, intracellular replication, and activation of inflammasome mediators during NMII infection. Full article

Oocyte–cumulus cell interaction is essential for oocyte maturation and competence. The bidirectional crosstalk network mediated by gap junctions is fundamental for the metabolic cooperation between these cells. As cumulus cells exhibit a more glycolytic phenotype, they can provide metabolic substrates that the oocyte can use to produce ATP via oxidative phosphorylation. The impairment of mitochondrial activity plays a crucial role in ovarian aging and, thus, in fertility, determining the success or failure of assisted reproductive techniques. This review aims to deepen the knowledge about the electro-metabolic coupling of the cumulus–oocyte complex and to hypothesize a putative role of potassium channel modulators in order to improve fertility, promote intracellular Ca²⁺ influx, and increase the mitochondrial biogenesis and resulting ATP levels in cumulus cells. Full article

The performance of technology companies listed on NASDAQ significantly impacts larger economic trends. Investors need specific information to navigate market volatility and make informed decisions in an increasingly complex marketplace. Furthermore, amidst the ongoing digital revolution, legislators and regulatory agencies must comprehend the operational dynamics of technology companies to develop frameworks that support innovation while maintaining market stability. Our study assesses the impact on the overall operational efficiency of NASDAQ-listed firms from 2011 to 2023, resulting from the interdependence of critical variables such as selling, general, and administrative expenses (SGA), cost of goods and services sold (COGS), and investments in research and development (R&D). Johansen’s cointegration methodology and pairwise Granger causality tests were employed to unveil long-term relationships, equilibrium adjustments, and causal relationships among the considered variables. The results provide critical insights into the strategic management of operational variables by the listed companies. The economic significance of the results obtained underscores the paramount importance of efficiently managing the cost of goods and services sold to achieve superior operating performance among these leading technology firms. Full article

The use of surfactants is crucial in the chemical–mechanical polishing fluid system for silicon wafers. This paper examines the impact of the functional group structure of polyoxyethylene-based nonionic surfactants and the variation in the polyoxyethylene (EO) addition number on the polishing performance of monocrystalline silicon wafers, to achieve the appropriate material removal rate and surface quality. The results demonstrated that the straight-chain structure of fatty alcohol polyoxyethylene ether (AEO-9) exhibited superior performance in wafer polishing compared to octylphenol polyoxyethylene ether (OP-9) and isoprenol polyoxyethylene ether (TPEG) and polyethylene glycol (PEG). By varying the number of EO additions of AEO-type surfactants, this study demonstrated that the polishing performance of monocrystalline silicon wafers was affected by the number of EO additions. The best polishing effect was achieved when the number of EO additions was nine. The mechanism of the role of polyoxyethylene-type nonionic surfactants in silicon wafer polishing was derived through polishing experiments, the contact angle, abrasive particle size analysis, zeta potential measurement, XPS, and other means of characterization. Full article

This study identified the practices of adaptation strategies to climate change in Jimma, Bedelle, Bonga, and Sokorru urban centers using a survey of 384 households, 55 key informant interviews, 4 focus group discussions, and field observations. A cross-sectional study design was employed from 2019 to 2021. The adaptive capacity of municipalities to reduce climate extreme events was rated as poor by the majority (51%), mostly reactive measures (76%). The climate hazards identified in four urban centers were riverine and flash floods, urban heat waves, landslides, and windstorms. The urban households practiced lifestyle modification, reduce paved surfaces, the use of air conditioner, planting trees, and multiple windows. The adaptation strategies practiced by municipalities include the relocation of prone areas, the support of basic amenities, the construction of protection walls, diversion ditches, the clearance of waterways and rivers, greenery, and park development. The adaptation actions were constrained by a lack of awareness, commitment, cooperation and coordination, adaptive capacity, and participation. Gray/physical infrastructures (costly but important) as adaptation actions were hampered by the low municipal capacity. We recommend that urban authorities should incorporate climate change adaptation strategies into urban planning and development proactively to ensure future resilient climate smart urban centers of southwest Ethiopia. Full article

The microbiota is in symbiosis with the human body as a holobiont. Infertility conditions affect the female reproductive tract (FRT) and its resident microbiota. However, a disturbance in homeostasis could influence the FRT and other distal body sites, such as the gastrointestinal tract (GIT). We included 21 patients with endometriosis and other infertility-associated diseases with clinical profiles and biological samples from the FRT (endometrium, endometrial fluid, and vagina), and GIT samples (oral and feces). We performed a 16S rRNA analysis of site-specific microbial communities and estimated diversity metrics. The study found body site-specific microbial patterns in the FRT–GIT. In both study groups, Lactobacillus was the most shared Amplicon Sequence Variant (ASV), a precise identifier of microbial sequences, between endometrial and vagina samples. However, shared Gardnerella and Enterobacteriaceae ASVs were linked to other conditions but not endometriosis. Remarkably, Haemophilus was a specific GIT-shared taxon in endometriosis cases. In conclusion, infertility influences distinctly the FRT and GIT microbiomes, with endometriosis showing unique microbial characteristics. We proposed the concept of ‘female holobiont’ as a community that comprises the host and microbes that must maintain overall homeostasis across all body sites to ensure a woman’s health. Insights into these microbial patterns not only advance our understanding of the pathophysiology of infertility but also open new avenues for developing microbe-based therapeutic interventions aimed at restoring microbial balance, thereby enhancing fertility prospects. Full article

This research aims to develop an efficient and accurate model for simulating tuned liquid dampers (TLDs) with sloped beds. The model, based on nonlinear shallow water equations, is enhanced by introducing new terms tailored to each specific case. It employs the central upwind method and Minmod limiter functions for flux and interface variable assessment, ensuring both high accuracy and reasonable computational cost. While acceleration, slope, and dissipation are treated as explicit sources, an implicit scheme is utilized for dispersion discretization to enhance the model’s stability, resulting in matrix equations. Time discretization uses the fourth-order Runge–Kutta scheme for precision. The performance of the model has been evaluated using several test cases including dam-breaks on flat and inclined beds and run-up and run-down simulations over parabolic beds, which are relevant to sloshing in tanks with sloped beds. It accurately predicts phenomena such as asymmetric sloshing waves, especially in sloped beds, where pronounced waves occur. Dispersion and dissipation terms are crucial for capturing these effects and maintaining stable wave patterns. An initial perturbation method assesses the tank’s natural period and numerical diffusion. Furthermore, the model integrates with a single-degree-of-freedom (SDOF) system to create a TLD model, demonstrating enhanced damping effects with sloped beds. Full article

The urban agglomeration on the north slope of the Tianshan Mountains is a pivotal place in Western China; it is essential for the economic growth of Xinjiang and acts as a critical bridge between China’s interior and the Asia–Europe continent. Due to unique natural conditions, the local population distribution exhibits distinct regional characteristics. This study employs the spatial lag model (SLM) from conventional spatial analysis and the random forest model (RFM) from contemporary machine learning techniques. It integrates traditional geographic data, including land cover data and nighttime light data, with geographical big data, such as POI (points of interest) and OSM (OpenStreetMap), to build a comprehensive indicator database. Subsequently, it simulates the spatial population distribution within the urban agglomeration on the northern slopes of the Tianshan Mountains in 2020. The accuracy of the results is then compared and assessed against the accuracy of other available population raster datasets, and the spatial distribution pattern in 2020 is analyzed. The findings reveal the following: (1) The result of SLM, combined with multi-source data, predicts the population distribution as a relatively uniform and nearly circular structure, with minimal spatial differentiation. (2) The result of RFM, employing multi-source data, better captures the spatial population distribution, resulting in irregular boundaries that are indicative of strong spatial heterogeneity. (3) Both models demonstrate superior accuracy in simulating population distribution. The spatial lag model’s accuracy surpasses that of the GHS and GPW datasets, albeit still trailing behind WorldPop and LandScan. Meanwhile, the random forest model significantly outperforms the four aforementioned population raster datasets. (4) The population spatial pattern in the urban agglomeration on the north slope of the Tianshan Mountains predominantly consists of four distinct circles, illustrating a “one axis, one center, and multiple focal points” distribution characteristic. Combining the random forest model with geographic big data for spatialized population simulation offers robust scientific validity and practicality. It holds potential for broader application within the urban agglomeration on the Tianshan Mountains and across Xinjiang. This study can offer insights for studies on regional population spatial distributions and inform sustainable development strategies for cities and their populations. Full article

Using biomass to develop and obtain environmentally friendly and industrially applicable biomaterials is increasingly attracting global interest. Herein, cellulose nanocrystals (CNCs) and lignin nanoparticles (LNPs) were extracted from Lemna minor L., a freshwater free-floating aquatic species commonly called duckweed. To obtain CNCs and LNPs, two different procedures and biomass treatment processes based on bleaching or on the use of an ionic liquid composed of triethylammonium and sulfuric acid ([TEA][HSO₄]), followed by acid hydrolysis, were carried out. Then, the effects of these treatments in terms of the thermal, morphological, and chemical properties of the CNCs and LNPs were assessed. The resulting nanostructured materials were characterized by using Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) spectroscopy, thermo-gravimetric analysis (TGA), and scanning electron microscopy (SEM). The results showed that the two methodologies applied resulted in both CNCs and LNPs. However, the bleaching-based treatment produced CNCs with a rod-like shape, length of 100–300 nm and width in the range of 10–30 nm, and higher purity than those obtained with ILs that were spherical in shape. In contrast, regarding lignin, IL made it possible to obtain spherical nanoparticles, as in the case of the other treatment, but they were characterized by higher purity and thermal stability. In conclusion, this research highlights the possibility of obtaining nanostructured biopolymers from an invasive aquatic species that is largely available in nature and how it is possible, by modifying experimental procedures, to obtain nanomaterials with different morphological, purity, and thermal resistance characteristics. Full article

Fryns syndrome (FS) is a multiple congenital anomaly syndrome with different multisystemic malformations. These include congenital diaphragmatic hernia, pulmonary hypoplasia, and craniofacial dysmorphic features in combination with malformations of the central nervous system such as agenesis of the corpus callosum, cerebellar hypoplasia, and enlarged ventricles. We present a non-consanguineous northern European family with two recurrent cases of FS: a boy with multiple congenital malformations who died at the age of 2.5 months and a female fetus with a complex developmental disorder with similar features in a following pregnancy. Quad whole exome analysis revealed two likely splicing-affecting disease-causing mutations in the PIGN gene: a synonymous mutation c.2619G>A, p.(Leu873=) in the last nucleotide of exon 29 and a 30 bp-deletion c.996_1023+2del (NM_176787.5) protruding into intron 12, with both mutations in trans configuration in the affected patients. Exon skipping resulting from these two variants was confirmed via RNA sequencing. Our molecular and clinical findings identified compound heterozygosity for two novel splice-affecting variants as the underlying pathomechanism for the development of FS in two patients. Full article

Ventilator-associated pneumonia (VAP) is common in Pediatric Intensive Care Units. Although early detection is crucial, current diagnostic methods are not definitive. This study aimed to identify lung ultrasound (LUS) findings and procalcitonin (PCT) values in pediatric patients with VAP to create a new early diagnosis score combined with the Clinical Pulmonary Infection Score (CPIS), the CPIS-PLUS score. Prospective longitudinal and interventional study. Pediatric patients with suspected VAP were included and classified into VAP or non-VAP groups, based on Centers of Disease Control (CDC) criteria for the final diagnosis. A chest-X-ray (CXR), LUS, and blood test were performed within the first 12 h of admission. CPIS score was calculated. A total of 108 patients with VAP suspicion were included, and VAP was finally diagnosed in 51 (47%) patients. CPIS-PLUS showed high accuracy in VAP diagnosis with a sensitivity (Sn) of 80% (95% CI 65–89%) and specificity (Sp) of 73% (95% CI 54–86%). The area under the curve (AUC) resulted in 0.86 for CPIS-PLUS vs. 0.61 for CPIS. In conclusion, this pilot study showed that CPIS-PLUS could be a potential and reliable tool for VAP early diagnosis in pediatric patients. Internal and external validations are needed to confirm the potential value of this score to facilitate VAP diagnosis in pediatric patients. Full article

Background/Objective: The relationship between heart rate and heart rate variability (HRV) indices has been repeatedly studied in adults but limited data are available on the relationship in paediatric populations. Methods: Continuous 12-lead electrocardiograms were recorded in 1016 healthy children and adolescents (534 females) aged 4 to 19 years during postural manoeuvres with rapid changes between 10-min positions of supine → sitting → standing → supine → standing → sitting → supine. In each position, the averaged RR interval was measured together with four HRV indices, namely the SDNN, RMSSD, quasi-normalised high-frequency components (qnHF), and the proportions of low- and high-frequency components (LF/HF). In each subject, the slope of the linear regression between the repeated HRV measurements and the corresponding RR interval averages was calculated. Results: The intra-subject regression slopes, including their confidence intervals, were related to the age and sex of the subjects. The SDNN/RR, RMSSD/RR, and qnHF/RR slopes were significantly steeper (p < 0.001) and the (LF/HF)/RR slopes were significantly shallower (p < 0.001) in younger children compared to older children and adolescents. Conclusions: The study suggests that sympathetic and vagal influences on heart rate are present in both younger and older children. With advancing age, the sympatho-vagal balance gradually develops and allows the vagal control to suppress the sympathetic drive towards higher heart rates seen in younger age children. Full article

Human defense against infection remains a global topic. In addition to developing novel anti-infection drugs, therapeutic drug delivery strategies are also crucial to achieving a higher efficacy and lower toxicity of these drugs for treatment. The application of hydrogels has been proven to be an effective localized drug delivery approach to treating infections without generating significant systemic adverse effects. The recent emerging dynamic hydrogels further show power as injectable formulations, giving new tools for clinical treatments. In this review, we delve into the potential applications of dynamic hydrogels in antibacterial and antiviral treatments and elaborate on their molecular designs and practical implementations. By outlining the chemical designs underlying these hydrogels, we discuss how the choice of dynamic chemical bonds affects their stimulus responsiveness, self-healing capabilities, and mechanical properties. Afterwards, we focus on how to endow dynamic hydrogels with anti-infection properties. By comparing different drug-loading methods, we highlight the advantages of dynamic chemical bonds in achieving sustained and controlled drug release. Moreover, we also include the design principles and uses of hydrogels that possess inherent anti-infective properties. Furthermore, we explore the design principles and applications of hydrogels with inherent anti-infective properties. Finally, we briefly summarize the current challenges faced by dynamic hydrogels and present a forward-looking vision for their future development. Through this review, we expect to draw more attention to these therapeutic strategies among scientists working with chemistry, materials, as well as pharmaceutics. Full article

Nsp13, a non-structural protein belonging to the coronavirus family 1B (SF1B) helicase, exhibits 5′–3′ polarity-dependent DNA or RNA unwinding using NTPs. Crucially, it serves as a key component of the viral replication–transcription complex (RTC), playing an indispensable role in the coronavirus life cycle and thereby making it a promising target for broad-spectrum antiviral therapies. The imidazole scaffold, known for its antiviral potential, has been proposed as a potential scaffold. In this study, a fluorescence-based assay was designed by labeling dsDNA substrates with a commercial fluorophore and monitoring signal changes upon Nsp13 helicase activity. Optimization and high-throughput screening validated the feasibility of this approach. In accordance with the structural characteristics of ADP, we employed a structural-based design strategy to synthesize three classes of imidazole-based compounds through substitution reaction. Through in vitro activity research, pharmacokinetic parameter analysis, and molecular docking simulation, we identified compounds A16 (IC₅₀ = 1.25 μM) and B3 (IC₅₀ = 0.98 μM) as potential lead antiviral compounds for further targeted drug research. Full article

Digital twins aim to optimize practices implemented in various sectors by bridging the gap between the physical and digital worlds. Focusing on open-field agriculture, livestock farming, and forestry and reviewing the current applications in these domains, this paper reveals the multifaceted roles of digital twins. Diverse key aspects are examined, including digital twin integration and maturity level, means of data acquisition, technological capabilities, and commonly used input and output features. Through the prism of four primary research questions, the state of the art of digital twins, the extent of their achieved integration, and an overview of the critical issues and potential advancements are provided in the landscape of the sectors under consideration. The paper concludes that in spite of the remarkable progress, there is a long way towards achieving full digital twin. Challenges still persist, while the key factor seems to be the integration of expert knowledge from different stakeholders. In light of the constraints identified in the review analysis, a new sector-specific definition for digital twins is also suggested to align with the distinctive characteristics of intricate biotic and abiotic systems. This research is anticipated to serve as a useful reference for stakeholders, enhancing awareness of the considerable benefits associated with digital twins and promoting a more systematic and comprehensive exploration of this transformative topic. Full article

In this article, we consider designs of simple analog artificial neural networks based on adiabatic Josephson cells with a sigmoid activation function. A new approach based on the gradient descent method is developed to adjust the circuit parameters, allowing efficient signal transmission between the network layers. The proposed solution is demonstrated on the example of a system that implements XOR and OR logical operations. Full article

In an era of increasing antibiotic resistance among pathogens, the treatment options for infectious diseases are diminishing. One of the clinical groups especially vulnerable to this threat are patients who are hospitalized in intensive care units due to ventilator-associated pneumonia caused by multidrug-resistant/extensively drug-resistant Gram-negative bacteria. In order to prevent the exhaustion of therapeutic options for this life-threatening condition, there is an urgent need for new pharmaceuticals. Novel β-lactam antibiotics, including combinations of cephalosporins with β-lactamase inhibitors, are proposed as a solution to this escalating problem. The unique mechanism of action, distinctive to this new group of siderophore cephalosporins, can overcome multidrug resistance, which is raising high expectations. In this review, we present the summarized results of clinical trials, in vitro studies, and case studies on the therapeutic efficacy of cefoperazone-sulbactam, ceftolozane-tazobactam, ceftazidime-avibactam, and cefiderocol in the treatment of ventilator-associated pneumonia. We demonstrate that treatment strategies based on siderophore cephalosporins and combinations of β-lactams with β-lactamases inhibitors show comparable or higher clinical efficacy than those used with classic pharmaceuticals, like carbapenems, colistin, or tigecycline, and are often associated with a lower risk of adverse events. Full article

Human cytomegalovirus (CMV) is a common herpesvirus causing lifelong latent infection in most people and is a primary cause of congenital infection worldwide. Given the role of NK cells in the materno-fetal barrier, we investigated peripheral blood NK cell behavior in the context of CMV infection acquired during pregnancy. We analyzed the NK phenotype and CD107a surface mobilization on PBMCs from CMV-transmitting and non-transmitting mothers and newborns with or without congenital infection. NK cells from non-transmitting mothers showed the typical phenotype of CMV-adaptive NK cells, characterized by higher levels of NKG2C, CD57, and KIRs, with reduced NKG2A, compared to transmitting ones. A significantly higher percentage of DNAM-1+, PD-1+, and KIR+NKG2A-CD57+PD-1+ CD56dim cells was found in the non-transmitting group. Accordingly, NK cells from congenital-CMV (cCMV)-infected newborns expressed higher levels of NKG2C and CD57, with reduced NKG2A, compared to non-congenital ones. Furthermore, they showed a significant expansion of CD56dim cells co-expressing NKG2C and CD57 or with a memory-like (KIR+NKG2A-CD57+NKG2C+) phenotype, as well as a significant reduction of the CD57-NKG2C- population. Degranulation assays showed a slightly higher CD107a geomean ratio in NK cells of mothers who were non-transmitting compared to those transmitting the virus. Our findings demonstrate that both CMV-transmitting mothers and cCMV newborns show a specific NK profile. These data can guide studies on predicting virus transmission from mothers and congenital infection in infants. Full article