The 2023 MDPI Annual Report has
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18 pages, 3289 KiB  
Article
Endophytic Fungal Diversity in Hardwickia binata: Bridging the Gap between Traditional and Modern Techniques
by Michael Joe Xavier Sneha, Myithili Thangavel, Israel Mani, Pandy Rajapriya, Nagendraprabhu Ponnuraj and Mohan Pandi
Microbiol. Res. 2024, 15(2), 823-840; https://doi.org/10.3390/microbiolres15020053 (registering DOI) - 16 May 2024
Abstract
Endophytic fungus is crucial for maintaining plant health and defense mechanisms, acting as protective barriers against pathogens, and producing medicinally beneficial bioactive compounds. Genome sequencing and metagenomics have significantly enhanced the understanding of fungal diversity and metabolic capabilities, enabling the identification of new [...] Read more.
Endophytic fungus is crucial for maintaining plant health and defense mechanisms, acting as protective barriers against pathogens, and producing medicinally beneficial bioactive compounds. Genome sequencing and metagenomics have significantly enhanced the understanding of fungal diversity and metabolic capabilities, enabling the identification of new genes and substances. Traditional culture-dependent methods have been complemented by culture-independent techniques, offering a more comprehensive view of fungal diversity. Using both culture-dependent and culture-independent techniques, the present research investigation explored the diversity of endophytic fungi encountered in the foliage of Hardwickia binata. The study examined the topographical characteristics and nutritional content of soil samples collected from the locality of the selected plant sample, H. binata, to better comprehend the effects on the plant’s growth. The balanced nutrient constituted approximately a pH of 7.2, which suggested an alkaline nature and promoted plant development. The ratio of nitrogen, phosphorous, and potassium remained 3:1:1. A total of 25 fungal isolates, categorized into 17 morphotypes, were obtained using the culture-dependent approach; Curvularia and Nigrospora emerged as the most common genera. Furthermore, the prediction of the ITS2 secondary structure supports the identification of species, highlighting a wide variety of fungal species present in H. binata. The culture-independent approach generated 69,570 high-quality sequences, identifying 269 Operational Taxonomic Units (OTUs). The dominant Ascomycota phylum, along with various genera, indicated a rich fungal community associated with H. binata. This study advances the understanding of the endophytic fungus communities that are associated with H. binata and the nature of soil ecology. The findings emphasize the significance of holistic techniques in the study of microbial dynamics within plant systems as well as their implications for ecosystem management and plant health. Full article
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17 pages, 5526 KiB  
Review
Scientific Research on Bioethanol in Brazil: History and Prospects for Sustainable Biofuel
by Adriana Grandis, Janaina da Silva Fortirer, Débora Pagliuso and Marcos S. Buckeridge
Sustainability 2024, 16(10), 4167; https://doi.org/10.3390/su16104167 (registering DOI) - 16 May 2024
Abstract
Despite the recent need for sustainable energy resources, bioenergy gained its spotlight in the 2000s. Sugarcane is a significant crop in terms of sugar and energy capacity, and it can be an alternative energy source to mitigate the effects of climate change. Bioenergy [...] Read more.
Despite the recent need for sustainable energy resources, bioenergy gained its spotlight in the 2000s. Sugarcane is a significant crop in terms of sugar and energy capacity, and it can be an alternative energy source to mitigate the effects of climate change. Bioenergy production from sugarcane in Brazil is one of the most efficient options. This production lends a centrality to biofuels’ importance in confronting climate change effects. The present article reviews the Brazilian history of this crop as a biofuel source, focusing on plants as a biomass. We highlight the historical changes related to scientific, technological, industrial, and environmental advances since the beginning of the 20th century. We describe how creating governmental institutes and disseminating scientific knowledge strengthened public policies that led Brazil to occupy leadership positions in producing, distributing, and using bioenergy throughout the country. The compiled data show the improvements and the new approaches needed to improve ethanol sugarcane use. We performed a bibliometric analysis to evaluate Brazilian science’s contribution to this process compared to other countries. Brazil’s history of science and investment in sugarcane biofuel development for transportation may be divided into two phases: ethanol-only and flex-fuel cars. A third phase is starting, directed to the SAF and ethanol-to-hydrogen era. Full article
(This article belongs to the Special Issue Recycling Biomass for Agriculture and Bioenergy Production)
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18 pages, 10152 KiB  
Article
Characteristics and Sources of CBM Well-Produced Water in the Shouyang Block, China
by Bing Zhang, Gang Wang, Wei Li and Xinglong Jiao
Appl. Sci. 2024, 14(10), 4218; https://doi.org/10.3390/app14104218 (registering DOI) - 16 May 2024
Abstract
The Shouyang Block was selected as the research subject. Comprehensive analysis was conducted using coalbed methane (CBM) well production data, geochemical test data on water produced from the coalbed methane well, and fundamental geological information. The findings reveal the water dynamics in the [...] Read more.
The Shouyang Block was selected as the research subject. Comprehensive analysis was conducted using coalbed methane (CBM) well production data, geochemical test data on water produced from the coalbed methane well, and fundamental geological information. The findings reveal the water dynamics in the Shouyang Block are characterized by weak groundwater runoff or retention in most areas. The groundwater head height exhibits a gradual decrease from the north to south, which is closely associated with the monoclinic structure of the Shouyang Block. Overall, water production is relatively high. As the average water production increases, the average gas production gradually decreases. A concentration of high water production wells is observed in the northern part of the Shouyang Block, which gradually increases towards the southeast direction. A comprehensive analysis was conducted on the factors influencing water production, including total water content of coal seams, coal seam porosity, groundwater stability index, groundwater sealing coefficient, D value of the fracture fractal dimension, fault fractal dimension, and sand–mud ratio. The correlation degree was calculated and ranked in order of magnitude through grey correlation analysis. The order of factors that influence water production, from strongest to weakest, is as follows: sand–mud ratio > porosity > fractal dimension of fault > fracture fractal dimension D value > groundwater sealing coefficient > groundwater stability index > total water content of coal seams. The dissolution amounts of carbonate and sulfate are both small, and the water source may mainly come from the sandstone aquifer. Attention should be paid to the distribution and lithological combination of sandstone aquifers in coal-bearing strata in the future exploration and development process of the Shouyang Block. This will help to avoid the potential influence of fault structures and enable the identification of favorable areas for low water and high gas production. Full article
(This article belongs to the Special Issue Advances in Unconventional Natural Gas: Exploration and Development)
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20 pages, 3941 KiB  
Article
Octoploids Show Enhanced Salt Tolerance through Chromosome Doubling in Switchgrass (Panicum virgatum L.)
by Jiali Ye, Yupu Fan, Hui Zhang, Wenjun Teng, Ke Teng, Juying Wu, Xifeng Fan, Shiwen Wang and Yuesen Yue
Plants 2024, 13(10), 1383; https://doi.org/10.3390/plants13101383 (registering DOI) - 16 May 2024
Abstract
Polyploid plants often exhibit enhanced stress tolerance. Switchgrass is a perennial rhizomatous bunchgrass that is considered ideal for cultivation in marginal lands, including sites with saline soil. In this study, we investigated the physiological responses and transcriptome changes in the octoploid and tetraploid [...] Read more.
Polyploid plants often exhibit enhanced stress tolerance. Switchgrass is a perennial rhizomatous bunchgrass that is considered ideal for cultivation in marginal lands, including sites with saline soil. In this study, we investigated the physiological responses and transcriptome changes in the octoploid and tetraploid of switchgrass (Panicum virgatum L. ‘Alamo’) under salt stress. We found that autoploid 8× switchgrass had enhanced salt tolerance compared with the amphidiploid 4× precursor, as indicated by physiological and phenotypic traits. Octoploids had increased salt tolerance by significant changes to the osmoregulatory and antioxidant systems. The salt-treated 8× Alamo plants showed greater potassium (K+) accumulation and an increase in the K+/Na+ ratio. Root transcriptome analysis for octoploid and tetraploid plants with or without salt stress revealed that 302 upregulated and 546 downregulated differentially expressed genes were enriched in genes involved in plant hormone signal transduction pathways and were specifically associated with the auxin, cytokinin, abscisic acid, and ethylene pathways. Weighted gene co-expression network analysis (WGCNA) detected four significant salt stress-related modules. This study explored the changes in the osmoregulatory system, inorganic ions, antioxidant enzyme system, and the root transcriptome in response to salt stress in 8× and 4× Alamo switchgrass. The results enhance knowledge of the salt tolerance of artificially induced homologous polyploid plants and provide experimental and sequencing data to aid research on the short-term adaptability and breeding of salt-tolerant biofuel plants. Full article
(This article belongs to the Special Issue Mechanism of Drought and Salinity Tolerance in Crops)
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15 pages, 7961 KiB  
Article
Mixed Reality Biopsy Navigation System Utilizing Markerless Needle Tracking and Imaging Data Superimposition
by Michał Trojak, Maciej Stanuch, Marcin Kurzyna, Szymon Darocha and Andrzej Skalski
Cancers 2024, 16(10), 1894; https://doi.org/10.3390/cancers16101894 (registering DOI) - 16 May 2024
Abstract
Exact biopsy planning and careful execution of needle injection is crucial to ensure successful procedure completion as initially intended while minimizing the risk of complications. This study introduces a solution aimed at helping the operator navigate to precisely position the needle in a [...] Read more.
Exact biopsy planning and careful execution of needle injection is crucial to ensure successful procedure completion as initially intended while minimizing the risk of complications. This study introduces a solution aimed at helping the operator navigate to precisely position the needle in a previously planned trajectory utilizing a mixed reality headset. A markerless needle tracking method was developed by integrating deep learning and deterministic computer vision techniques. The system is based on superimposing imaging data onto the patient’s body in order to directly perceive the anatomy and determine a path from the selected injection site to the target location. Four types of tests were conducted to assess the system’s performance: measuring the accuracy of needle pose estimation, determining the distance between injection sites and designated targets, evaluating the efficiency of material collection, and comparing procedure time and number of punctures required with and without the system. These tests, involving both phantoms and physician participation in the latter two, demonstrated the accuracy and usability of the proposed solution. The results showcased a significant improvement, with a reduction in number of punctures needed to reach the target location. The test was successfully completed on the first attempt in 70% of cases, as opposed to only 20% without the system. Additionally, there was a 53% reduction in procedure time, validating the effectiveness of the system. Full article
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15 pages, 778 KiB  
Article
Schēma: A Semantic Puzzle—Some Hermeneutical and Translational Difficulties about Philippians 2:7d
by Teresa Bartolomei
Religions 2024, 15(5), 613; https://doi.org/10.3390/rel15050613 (registering DOI) - 16 May 2024
Abstract
The occurrence of the term σχήμα in Phil 2:7d is analyzed in comparison with two other crucial Pauline occurrences: 1 Cor 7:31 and Phil 3:21 (here as a semanteme included in the verb μετασχηματίσει). This comparative study aims to provide a revision [...] Read more.
The occurrence of the term σχήμα in Phil 2:7d is analyzed in comparison with two other crucial Pauline occurrences: 1 Cor 7:31 and Phil 3:21 (here as a semanteme included in the verb μετασχηματίσει). This comparative study aims to provide a revision of the current interpretation of the word as designating the outward, sensory, accidental appearance in which Christ’s human nature was manifested to those who dealt with him. This traditional reconstruction is unsatisfactory in two respects: (1) it is tributary to a substantialist ontology that identifies corporeality as a mere spatial extension, unrelated to historicity and (2) it is fraught with highly problematic theological, potentially docetic, implications. As an alternative, the term σχήμα is here interpreted within the framework of the great Pauline theology of history: as a temporal–eschatological marker designating the peculiar temporal state of transience and suffering corruptibility inherent in physicality and corporeal life. This change also clarifies the conceptual articulation of σχήμα with the parallel expression μορφὴν δούλου. According to this interpretation, contrary to the prevailing view, the locution “slave form” does not designate ‘the’ or ‘one’ ‘human form’ but the ‘creature form’, as cosmic submission to temporal finitude. Full article
(This article belongs to the Special Issue Current Trends in Pauline Research: Philippians)
19 pages, 10454 KiB  
Article
Simulation and Management Impact Evaluation of Debris Flow in Dashiling Gully Based on FLO-2D Modeling
by Xiamin Jia, Jianguo Lv and Yaolong Luo
Appl. Sci. 2024, 14(10), 4216; https://doi.org/10.3390/app14104216 (registering DOI) - 16 May 2024
Abstract
Dashiling Gully, located in Miyun District, Beijing, exhibits a high susceptibility to debris flow due to its unique geological and topographical characteristics. The area is characterized by well-developed rock joints and fissures, intense weathering, a steep gradient, and a constricted gully morphology. These [...] Read more.
Dashiling Gully, located in Miyun District, Beijing, exhibits a high susceptibility to debris flow due to its unique geological and topographical characteristics. The area is characterized by well-developed rock joints and fissures, intense weathering, a steep gradient, and a constricted gully morphology. These factors contribute to the accumulation of surface water and loose sediment, significantly increasing the risk of debris flow events. Following a comprehensive field geological investigation of Dashiling Gully, key parameters for simulation were obtained, including fluid weight, volume concentration, and rainfall. The formation and development conditions of potential mudslides were analyzed, and numerical simulations were conducted using FLO-2D software (version 2009) to assess scenarios with rainfall probabilities of 1 in 30, 50, and 100 years. The simulations accurately reconstructed the movement velocity, deposition depth, and other critical movement characteristics of mudslides under each rainfall scenario. Using ArcGIS, pre- and post-treatment hazard zoning maps were generated for Dashiling Gully. Furthermore, the efficacy of implementing a retaining wall as a mitigation measure was evaluated through additional numerical simulations. The results indicated that mudslide velocities ranged from 0 to 3 m/s, with deposition depths primarily between 0 and 3 m. The maximum recorded velocity reached 3.5 m/s, corresponding to a peak deposition depth of 4.31 m. Following the implementation of the retaining wall, the maximum deposition depth significantly decreased to 1.9 m, and high-risk zones were eliminated, demonstrating the intervention’s effectiveness. This study provides a rigorous evaluation of mudslide movement characteristics and the impact of mitigation measures within Dashiling Gully. The findings offer valuable insights and serve as a reference for forecasting and mitigating similar mudslide events triggered by heavy rainfall in gully mudslides. Full article
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14 pages, 2678 KiB  
Article
Multi-Objective Synergetic Operation for Cascade Reservoirs in the Upper Yellow River
by Kunhui Hong, Wei Zhang, Aixing Ma, Yucong Wei and Mingxiong Cao
Water 2024, 16(10), 1416; https://doi.org/10.3390/w16101416 (registering DOI) - 16 May 2024
Abstract
The Yellow River, a critical water resource, faces challenges stemming from increasing water demand, which has led to detrimental effects on hydropower generation and ecological balance. This paper will address the complex task of balancing the interests of hydropower generation, water supply, and [...] Read more.
The Yellow River, a critical water resource, faces challenges stemming from increasing water demand, which has led to detrimental effects on hydropower generation and ecological balance. This paper will address the complex task of balancing the interests of hydropower generation, water supply, and ecology within the context of cascade reservoirs, specifically Longyangxia and Liujiaxia reservoirs. Employing a systemic coupling coordination approach, we constructed a multi-objective synergetic model of the upper Yellow River in order to explore synergies and competitions among multiple objectives. The results reveal that there is a weak competitive relationship between hydropower generation and water supply, a strong synergy between hydropower generation and ecology, and a strong competitive relationship between water supply and ecology. The Pareto solution set analysis indicates a considerable percentage (59%, 20%, and 8% in wet, normal, and dry years, respectively) exhibiting excellent coordination. The probability of excellent coordination decreases with diminishing inflow. The optimization scheme with the highest coupling coordination demonstrates significant improvements in power generation, water supply, and ecological benefits in the upper Yellow River without compromising other objectives, fostering the sustainable operation of hydropower generation, water supply, and ecology in the upper Yellow River. Full article
(This article belongs to the Section Water Resources Management, Policy and Governance)
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13 pages, 1803 KiB  
Case Report
Challenging Molecular Diagnosis of Congenital Adrenal Hyperplasia (CAH) Due to 21-Hydroxylase Deficiency: Case Series and Novel Variants of CYP21A2 Gene
by Paola Concolino
Curr. Issues Mol. Biol. 2024, 46(5), 4832-4844; https://doi.org/10.3390/cimb46050291 (registering DOI) - 16 May 2024
Abstract
Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive genetic defects in cortisol synthesis and shows elevated ACTH concentrations, which in turn has downstream effects. The most common variant of CAH, 21-hydroxylase deficiency (21OHD), is the result of pathogenic variants in the [...] Read more.
Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive genetic defects in cortisol synthesis and shows elevated ACTH concentrations, which in turn has downstream effects. The most common variant of CAH, 21-hydroxylase deficiency (21OHD), is the result of pathogenic variants in the CYP21A2 gene and is one of the most common monogenic disorders. However, the genetics of 21OHD is complex and challenging. The CYP21A2 gene is located in the RCCX copy number variation (CNV), a complex, multiallelic, and tandem CNV in the major histocompatibility complex (MHC) class III region on chromosome 6 (band 6p21.3). Here, CYP21A2 and its pseudogene CYP21A1P are located 30 kb apart and share a high nucleotide homology of approximately 98% and 96% in exons and introns, respectively. This high-sequence homology facilitates large structural rearrangements, copy number changes, and gene conversion through intergenic recombination. There is a good genotype–phenotype correlation in 21OHD, and genotyping can be performed to confirm the clinical diagnosis, predict long-term outcomes, and determine genetic counseling. Thus, genotyping in CAH is clinically relevant but the interpretations can be challenging for non-initiated clinicians. Here, there are some concrete examples of how molecular diagnosis can sometimes require the use of multiple molecular strategies. Full article
(This article belongs to the Special Issue Complex Molecular Mechanism of Monogenic Diseases 2.0)
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11 pages, 1042 KiB  
Article
The Association of Serum Uric Acid with Risk of Obstructive Sleep Apnea: The Korean National Health and Nutrition Examination Survey 2019–2021
by Su-Lim Park, Jihye Lim and Ji-Ho Lee
J. Pers. Med. 2024, 14(5), 532; https://doi.org/10.3390/jpm14050532 (registering DOI) - 16 May 2024
Abstract
Upper airway collapse and apneas in obstructive sleep apnea (OSA) induce intermittent hypercapnia and hypoxia, eventually contributing to excessive uric acid production. This study aimed to evaluate the association between hyperuricemia and OSA in the general population via analysis of the eighth KNHANES [...] Read more.
Upper airway collapse and apneas in obstructive sleep apnea (OSA) induce intermittent hypercapnia and hypoxia, eventually contributing to excessive uric acid production. This study aimed to evaluate the association between hyperuricemia and OSA in the general population via analysis of the eighth KNHANES dataset (2019–2021). OSA risk was identified via the STOP-Bang questionnaire, with a score ≥3 indicating high risk. Among 11,981 total participants, 4572 (38.2%) had a high OSA risk. Participants with a high OSA risk had higher uric acid levels compared to those with a low risk (5.5 ± 1.4 mg/dL vs. 4.8 ± 1.2 mg/dL, p < 0.001). Serum uric acid levels were positively correlated with STOP-Bang score (r: 0.317, p < 0.001). Multivariate analysis revealed that hyperuricemia was associated with a high OSA risk after adjusting for confounders (odds ratio: 1.30, 95%CI: 1.11–1.53, p = 0.001). Therefore, serum uric acid levels are significantly higher in those with a high OSA risk and correlate with the risk of OSA. Further, hyperuricemia is an independently associated risk factor for high OSA risk. More research is warranted to evaluate the long-term clinical outcomes of hyperuricemia in OSA and to determine whether treatment targeting hyperuricemia is effective in the clinical course of OSA. Full article
(This article belongs to the Section Clinical Medicine, Cell, and Organism Physiology)
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15 pages, 310 KiB  
Article
Positive-Energy Dirac Particles and Dark Matter
by Eugene Bogomolny
Universe 2024, 10(5), 222; https://doi.org/10.3390/universe10050222 (registering DOI) - 16 May 2024
Abstract
The relativistic positive-energy wave equation proposed by P. Dirac in 1971 is an old but largely forgotten subject. The purpose of this note is to speculate that particles described by this equation (called here Dirac particles) are natural candidates for the dark matter. [...] Read more.
The relativistic positive-energy wave equation proposed by P. Dirac in 1971 is an old but largely forgotten subject. The purpose of this note is to speculate that particles described by this equation (called here Dirac particles) are natural candidates for the dark matter. The reasoning is based on a fact that the internal structure of such particles simply prohibits their interaction with electromagnetic fields (at least with the minimal coupling) which is exactly what is required for dark matter. Dirac particles have quite unusual properties. In particular, they are transformed by an infinite-dimensional representation of the homogeneous Lorentz group, which clearly distinguishes them from all known elementary particles described by finite-dimensional representations and hints to a physics beyond the Standard Model. To clarify the topic, a brief review of the main features of the above-mentioned Dirac equation is given. Full article
(This article belongs to the Section Cosmology)
15 pages, 5191 KiB  
Article
Hypersalinity in Coastal Wetlands and Potential Restoration Solutions, Lake Austin and East Matagorda Bay, Texas, USA
by Rusty A. Feagin, Joshua E. Lerner, Caroline Noyola, Thomas P. Huff, Jake Madewell and Bill Balboa
J. Mar. Sci. Eng. 2024, 12(5), 829; https://doi.org/10.3390/jmse12050829 (registering DOI) - 16 May 2024
Abstract
When droughts occur, freshwater inputs to coastal wetlands can become scarce and hypersalinity can become a problem. In 2023, a severe drought negatively affected a Texas watershed known as Lake Austin that fed a large expanse of wetlands on East Matagorda Bay. To [...] Read more.
When droughts occur, freshwater inputs to coastal wetlands can become scarce and hypersalinity can become a problem. In 2023, a severe drought negatively affected a Texas watershed known as Lake Austin that fed a large expanse of wetlands on East Matagorda Bay. To study the hypersalinity problem in these wetlands, we identified freshwater inflows and mapped vegetation changes over time. We found that from 1943 to 2023, the upper portion of the Lake Austin watershed lost freshwater wetlands to agricultural conversion, and ranged from fresh to brackish, with salinity rapidly rising to a maximum of 31 mS during the summer drought of 2023. The lower portion of the watershed gained saltwater wetlands due to sea level rise, and marshes became hypersaline (64–96 mS) during the 2023 drought, endangering its biota. But after large precipitation events, the entire Lake Austin basin rapidly freshened but then returned to its normal salinities within a week as the tides re-delivered saltwater into its basin. Given current climatic trends, we expect that freshwater inflow will continue to slightly increase for the Lake Austin watershed but also that there will be more extreme periods of episodic drought that negatively affect its wetlands. Accordingly, we assessed several potential restoration actions that would improve freshwater flow and delivery to the Lake Austin coastal wetlands. Full article
(This article belongs to the Section Marine Environmental Science)
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16 pages, 11592 KiB  
Article
Deep-Reinforcement-Learning-Based Collision Avoidance of Autonomous Driving System for Vulnerable Road User Safety
by Haochong Chen, Xincheng Cao, Levent Guvenc and Bilin Aksun-Guvenc
Electronics 2024, 13(10), 1952; https://doi.org/10.3390/electronics13101952 (registering DOI) - 16 May 2024
Abstract
The application of autonomous driving system (ADS) technology can significantly reduce potential accidents involving vulnerable road users (VRUs) due to driver error. This paper proposes a novel hierarchical deep reinforcement learning (DRL) framework for high-performance collision avoidance, which enables the automated driving agent [...] Read more.
The application of autonomous driving system (ADS) technology can significantly reduce potential accidents involving vulnerable road users (VRUs) due to driver error. This paper proposes a novel hierarchical deep reinforcement learning (DRL) framework for high-performance collision avoidance, which enables the automated driving agent to perform collision avoidance maneuvers while maintaining appropriate speeds and acceptable social distancing. The novelty of the DRL method proposed here is its ability to accommodate dynamic obstacle avoidance, which is necessary as pedestrians are moving dynamically in their interactions with nearby ADSs. This is an improvement over existing DRL frameworks that have only been developed and demonstrated for stationary obstacle avoidance problems. The hybrid A* path searching algorithm is first applied to calculate a pre-defined path marked by waypoints, and a low-level path-following controller is used under cases where no VRUs are detected. Upon detection of any VRUs, however, a high-level DRL collision avoidance controller is activated to prompt the vehicle to either decelerate or change its trajectory to prevent potential collisions. The CARLA simulator is used to train the proposed DRL collision avoidance controller, and virtual raw sensor data are utilized to enhance the realism of the simulations. The model-in-the-loop (MIL) methodology is utilized to assess the efficacy of the proposed DRL ADS routine. In comparison to the traditional DRL end-to-end approach, which combines high-level decision making with low-level control, the proposed hierarchical DRL agents demonstrate superior performance. Full article
(This article belongs to the Special Issue Intelligent Technologies for Vehicular Networks)
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16 pages, 8324 KiB  
Article
Temperature Prediction of Icy Lunar Soil Sampling Based on the Discrete Element Method
by Deming Zhao, Tianyi Peng, Weiwei Zhang, He Wang and Jinsheng Cui
Aerospace 2024, 11(5), 400; https://doi.org/10.3390/aerospace11050400 (registering DOI) - 16 May 2024
Abstract
This study is part of the preliminary research for the Chang’e 7 project in China. The Chang’e 7 project plans to drill to penetrate the lunar polar soil and collect lunar soil samples using a spiral groove structure. Ice in the cold environment [...] Read more.
This study is part of the preliminary research for the Chang’e 7 project in China. The Chang’e 7 project plans to drill to penetrate the lunar polar soil and collect lunar soil samples using a spiral groove structure. Ice in the cold environment of the lunar polar region is one of the important targets for sampling. In the vacuum environment of the lunar surface, icy soil samples are sensitive to ambient temperature and prone to solid–gas phase change as the temperature increases. To predict the temperature range of lunar soil samples, this study analyzed the effect of thermal parameters on the temperature rise of lunar soil particles and the drill using discrete element simulation. The parameters included in the thermal effect analysis included the thermal conductivity and specific heat capacity of the drilling tools and lunar soil particles. The simulation showed that the temperature of the icy lunar soil sample in the spiral groove ranged from −127.89 to −160.16 °C within the thermal parameter settings. The magnitude of the value was negatively correlated with the thermal conductivity and specific heat capacity of the lunar soil particles, and it was positively correlated with those of the drilling tools. The temperature variation in the drill bit ranged from −51.21 to −132 °C. The magnitude of the value was positively correlated with the thermal conductivity and specific heat capacity of the lunar soil particles and the thermal conductivity of the drilling tool. Full article
(This article belongs to the Special Issue Space Sampling and Exploration Robotics)
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22 pages, 6004 KiB  
Article
Coupling Global Parameters and Local Flow Optimization of a Pulsed Ejector for Proton Exchange Membrane Fuel Cells
by Chao Li, Baigang Sun and Lingzhi Bao
Sustainability 2024, 16(10), 4170; https://doi.org/10.3390/su16104170 (registering DOI) - 16 May 2024
Abstract
Proton exchange membrane fuel cells (PEMFCs), as an important utilization of hydrogen energy, contribute to the sustainable development of global energy. Pulsed ejectors have a high potential for improving the hydrogen utilization of PEMFCs in the full operating range by circulating unconsumed hydrogen. [...] Read more.
Proton exchange membrane fuel cells (PEMFCs), as an important utilization of hydrogen energy, contribute to the sustainable development of global energy. Pulsed ejectors have a high potential for improving the hydrogen utilization of PEMFCs in the full operating range by circulating unconsumed hydrogen. In this study, a pulsed ejector applied to a 120 kW fuel cell was designed, and the flow characteristics were analysed using computational fluid dynamics (CFD). Based on the data from the CFD model, the global optimization of the ejector was carried out using the Gaussian process regression (GPR) surrogate model and the grey wolf optimization (GWO) algorithm. The local structure was then further optimized using an adjoint method coupling streamlining modification that takes into account the local flow characteristics. The CFD results showed that, under a fixed structure, increasing the pressure difference between the secondary flow and the ejector outlet would promote boundary layer separation, shorten the shockwave chain length, change the effective flow area of the secondary flow, and lower the entrainment ratio (ER). The analytical results from the GPR model indicated significant interactions among the structural parameters. The globally optimized ejector using GPR and GWO improved the hydrogen entrainment ratio from 1.42 to 3.12 at the design point. Furthermore, the results of streamlining local optimization show that the entrainment ratio increased by 1.67% at the design point and increased by up to 3.99% over the full operating range compared to the optimized ejector by global optimization. Full article
(This article belongs to the Special Issue Low-Carbon Transportation)
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17 pages, 258 KiB  
Article
Entrepreneurial Aspirations and Challenges among Business Students: A Qualitative Study
by Anas Al-Fattal
Adm. Sci. 2024, 14(5), 101; https://doi.org/10.3390/admsci14050101 (registering DOI) - 16 May 2024
Abstract
The COVID-19 pandemic has had a profound impact on small businesses, significantly influencing entrepreneurial aspirations and presenting numerous challenges. This calls for additional research into perceptions, intentions, and the challenges faced in this context. This study aims to explore the comprehension of key [...] Read more.
The COVID-19 pandemic has had a profound impact on small businesses, significantly influencing entrepreneurial aspirations and presenting numerous challenges. This calls for additional research into perceptions, intentions, and the challenges faced in this context. This study aims to explore the comprehension of key entrepreneurial concepts among business students in the post-pandemic era. The paper presents an empirical study which employs qualitative in-depth interviews with 34 undergraduate business students from one public university in the Midwest of the United States. The findings reveal a complex view of entrepreneurship that extends beyond traditional business creation, encompassing elements of social innovation and personal fulfillment. Students displayed a generally positive attitude towards entrepreneurship, influenced strongly by their involvement in practical entrepreneurship-related activities and their familial backgrounds. However, they also identified significant barriers, including financial constraints, fear of failure, and a lack of practical experience, which hinder their intentions to pursue entrepreneurial ventures. The study underscores the importance of entrepreneurship education programs incorporating more comprehensive practical experiences, enhancing financial literacy, and providing psychological support to overcome these challenges. These insights contribute to the ongoing discussion on how to effectively support and prepare aspiring entrepreneurs in a changing educational landscape. Full article
(This article belongs to the Special Issue Moving from Entrepreneurial Intention to Behavior)
15 pages, 854 KiB  
Article
Secure and Scalable Internet of Things Model Using Post-Quantum MACsec
by Juhee Choi and Junwon Lee
Appl. Sci. 2024, 14(10), 4215; https://doi.org/10.3390/app14104215 (registering DOI) - 16 May 2024
Abstract
For the secure deployment of network platforms tailored for IoT devices, the encryption of data transmission is equally as crucial as the process of authentication. In this context, we introduce the Secure and Scalable IoT network (SSI) network platform, designed to accommodate a [...] Read more.
For the secure deployment of network platforms tailored for IoT devices, the encryption of data transmission is equally as crucial as the process of authentication. In this context, we introduce the Secure and Scalable IoT network (SSI) network platform, designed to accommodate a diverse range of IoT devices. It provides scalability and implements effective many-to-many and end-to-end encryption across extensive regions. With the emergence of quantum computing, secure public key exchange mechanisms have become important. Among the various post-quantum cryptography (PQC) algorithms assessed, Nth Degree Truncated Polynomial Ring Units (NTRUs) have emerged as an optimally suited PQC algorithm for IoT devices constrained by limited computational capabilities. We have integrated NTRUs with SSI as a lightweight PQC solution. Moreover, SSI-PQM (Post-Quantum MACsec) enhances the SSI’s initial authentication structure to minimize PQC-TLS session attempts and protect the SSI’s important configuration information. When applying TLS with PQC for secret key exchange purposes, it was verified that this approach ensures stable performance in IoT environments. Upon the implementation of our proposed SSI-PQM on Raspberry Pi 3B+ based IoT devices, SSI-PQM exhibited acceptable performance at security levels from 80 to 128 and achieved a minimum speed improvement of 161% over RSA at security levels above 160. It can be concluded that SSI-PQM stands out as an effective Zero Trust-based IoT network platform, demonstrating its viability and efficiency in safeguarding data transmission against potential quantum computing threats. Full article
(This article belongs to the Special Issue Advanced Technologies in Data and Information Security III)
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11 pages, 1030 KiB  
Article
The Expression of TP63 as a Biomarker of Early Recurrence in Resected Esophageal Squamous Cell Carcinoma after Neoadjuvant Chemoradiotherapy
by Chih-Hung Lin, Po-Liang Cheng, Cheng-Yeh Chuang, Yu-Ting Kang, Li-Wen Lee, Tzu-Hung Hsiao and Chung-Ping Hsu
Biomedicines 2024, 12(5), 1101; https://doi.org/10.3390/biomedicines12051101 (registering DOI) - 16 May 2024
Abstract
Esophageal cancer ranks among the ten most common cancers worldwide. Despite the adoption of neoadjuvant concurrent chemoradiotherapy (nCCRT) followed by surgery as the standard treatment approach in recent years, the local recurrence rate remains high. In this study, we employed RNA-seq to investigate [...] Read more.
Esophageal cancer ranks among the ten most common cancers worldwide. Despite the adoption of neoadjuvant concurrent chemoradiotherapy (nCCRT) followed by surgery as the standard treatment approach in recent years, the local recurrence rate remains high. In this study, we employed RNA-seq to investigate distinctive gene expression profiles in esophageal squamous cell carcinoma (ESCC) with or without recurrence following a standard treatment course. Our findings indicate that recurrent ESCC exhibits heightened keratinizing and epidermis development activity compared to non-recurrent ESCC. We identified TP63 as a potential candidate for distinguishing clinical outcomes. Furthermore, immunohistochemistry confirmed the trend of TP63 overexpression in ESCC recurrence. Patients with elevated TP63 expression had poorer overall survival and lower 3-year recurrence-free survival. This study underscores the potential of TP63 as a biomarker for detecting cancer recurrence and suggests its role in guiding future treatment options. Full article
(This article belongs to the Section Cancer Biology and Oncology)
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15 pages, 1554 KiB  
Article
Untangling the Defiant Taxonomy of Physaloptera (Nematoda: Chromadorea: Spirurida: Physalopteridae) Parasites in Reptiles: An Integrative Approach on the Enigmatic P. retusa Suggests Cryptic Speciation
by Lorena Gisela Ailán-Choke, Vanda Lúcia Ferreira, Fernando Paiva, Luiz Eduardo Roland Tavares, Fabiano Paschoal and Felipe Bisaggio Pereira
Taxonomy 2024, 4(2), 326-340; https://doi.org/10.3390/taxonomy4020016 (registering DOI) - 16 May 2024
Abstract
(1) Background: Although Physaloptera retusa is one of the most widespread species infecting reptiles in the Americas, numerous taxonomic problems and little genetic data are associated with it. To clarify the taxonomy of this species, we used an integrative approach. (2) Methods: Physaloptera [...] Read more.
(1) Background: Although Physaloptera retusa is one of the most widespread species infecting reptiles in the Americas, numerous taxonomic problems and little genetic data are associated with it. To clarify the taxonomy of this species, we used an integrative approach. (2) Methods: Physaloptera retusa-infecting Erythrolamprus typhlus (snake) from the Pantanal wetlands, Brazil, was morphologically and genetically characterised (18S and 28S rDNA; COI mtDNA) and compared with conspecific sequences available in GenBank, from parasites of Tupinambis teguixin (lizard), using species delimitation methods. Type specimens of P. liophis were re-evaluated given its morphological similarities with P. retusa. (3) Results: The morphology of the present specimens was equal to that of P. retusa, in which the only difference from P. liophis was the relative position of the vulva. Species delimitation methods were more accurate for the COI dataset; all of them (except ABGD) indicated interspecificity among P. retusa sequences. However, a lack of morphological data or voucher material, associated with the deposited sequences, prevented more assertive conclusions. (4) Conclusions: The present results highlight the importance of a clear association between genetic data and morphology of the isolation source, or at least its adequate vouchering. Moreover, P. retusa may represent a species complex in cryptic speciation, since it is widespread and has low hosts specificity. Full article
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12 pages, 2402 KiB  
Article
One-Step Synthesis of High-Efficiency Oxygen Evolution Reaction Catalyst FeSx(Y/MB) with High Temperature Resistance and Strong Alkali
by Jing Wang, Lingling Feng, Zikang Zhao, Yan Wang, Ying Zhang, Shan Song, Shengwei Sun, Junshuang Zhou and Faming Gao
Catalysts 2024, 14(5), 324; https://doi.org/10.3390/catal14050324 (registering DOI) - 16 May 2024
Abstract
Given the energy crisis and escalating environmental pollution, the imperative for developing clean new energy is evident. Hydrogen has garnered significant attention owing to its clean properties, high energy density, and ease of storage and transportation. This study synthesized four types of catalysts—FeS(DI/MB), [...] Read more.
Given the energy crisis and escalating environmental pollution, the imperative for developing clean new energy is evident. Hydrogen has garnered significant attention owing to its clean properties, high energy density, and ease of storage and transportation. This study synthesized four types of catalysts—FeS(DI/MB), FeS(ET/MB), Fe(DI/MB), and Fe(ET/MB)—using two distinct solution systems: DI/MB and ET/MB. The FeS(DI/MB) catalyst, synthesized using the layered solution system (DI/MB), demonstrates a uniformly distributed and dense nanosheet structure, exhibiting excellent resistance to strong bases and superior catalytic properties. The FeS(DI/MB) electrode showed OER overpotentials of 460 mV and 318 mV in 1 M and 6 M, respectively, at current densities of up to 500 mA cm−2. Under industrial electrolysis test conditions, the FeS(DI/MB) electrode required only 262 mV to achieve a current density of 500 mA cm−2, operating in a high-temperature, strong alkaline environment of 6 M at 60 °C. Furthermore, the FeS(DI/MB) electrode exhibited excellent OER catalytic activity and stability, as evidenced by a 60 h stability test These findings provide valuable insights into the preparation of iron nickel sulfide-based catalysts, and further in-depth and comprehensive exploration is anticipated to yield the excellent catalytic performance of these catalysts in the realm of electrolytic water hydrogen production. Full article
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17 pages, 227 KiB  
Article
Knowledge Assessment Patterns for Distant Education: The Perceived Impact on Grading, Motivation, and Satisfaction
by Tina Beranič, Marjan Heričko and Luka Pavlič
Sustainability 2024, 16(10), 4166; https://doi.org/10.3390/su16104166 (registering DOI) - 16 May 2024
Abstract
With the increase in remote learning, the efficient implementation of distant knowledge assessments has become an essential topic. New challenges have arisen that have had to be adequately addressed and successfully solved. As a response, we introduced an assessment patterns catalogue for distant [...] Read more.
With the increase in remote learning, the efficient implementation of distant knowledge assessments has become an essential topic. New challenges have arisen that have had to be adequately addressed and successfully solved. As a response, we introduced an assessment patterns catalogue for distant education, handling various challenges by proposing possible solutions. The catalogue presents a collection of proven practises targeting knowledge assessment in digital and distant environments. The paper presents the survey results from the final step of catalogue creation. The objective was to verify its suitability and expedite its use, focusing on several aspects of knowledge assessment. We focused on the perceived assessment patterns’ impact on grading objectivity and consistency, as well as students’ motivation and satisfaction with an implemented assessment, explored from the students’ and teachers’ perspectives. We gathered data using a uniform questionnaire distributed between students and teachers, both actively involved in distant knowledge assessment. Detailed data analysis highlighted the patterns with the highest perceived impact on the previously mentioned assessment aspects. We also analysed the top-rated patterns within the pattern categories. The results depict a high overlap between students’ and teachers’ perspectives, wherein patterns like Pentathlon, Statistical Validator, Game Rules, and Bonus Points were perceived as the patterns with the highest impact on grading objectivity and consistency, as well as the patterns with the most significant impact on students’ motivation and satisfaction. Full article
(This article belongs to the Special Issue Sustainable Higher Education: From E-learning to Smart Education)
19 pages, 3601 KiB  
Article
First Pangenome of Corynebacterium rouxii, a Potentially Toxigenic Species of Corynebacterium diphtheriae Complex
by Fernanda Diniz Prates, Max Roberto Batista Araújo, Eduarda Guimarães Sousa, Juliana Nunes Ramos, Marcus Vinícius Canário Viana, Siomar de Castro Soares, Louisy Sanches dos Santos and Vasco Ariston de Carvalho Azevedo
Bacteria 2024, 3(2), 99-117; https://doi.org/10.3390/bacteria3020007 (registering DOI) - 16 May 2024
Abstract
Corynebacterium rouxii is one of the recently described species of the Corynebacterium diphtheriae complex. As this species can potentially infect different hosts and harbor the tox gene, producing diphtheria toxin, we present its first pangenomic analysis in this work. A total of fifteen [...] Read more.
Corynebacterium rouxii is one of the recently described species of the Corynebacterium diphtheriae complex. As this species can potentially infect different hosts and harbor the tox gene, producing diphtheria toxin, we present its first pangenomic analysis in this work. A total of fifteen genomes deposited in online databases were included. After confirming the taxonomic position of the isolates by genomic taxonomy, the genomes were submitted to genomic plasticity, gene synteny, and pangenome prediction analyses. In addition, virulence and antimicrobial resistance genes were investigated. Finally, epidemiological data were obtained through molecular typing, clustering, and phylogenetic analysis. Our data demonstrated genetic diversity within the species with low synteny. However, the gene content is extensively conserved, and the pangenome is composed of 2606 gene families, of which 1916 are in the core genome and 80 are related to unique genes. Prophages, insertion sequences, and genomic islands were found. A type I-E CRISPR-Cas system was also detected. Besides the tox gene, determinants involved in adhesion and iron acquisition and two putative antimicrobial resistance genes were predicted. These findings provide valuable insight about this species’ pathogenicity, evolution, and diversity. In the future, our data can contribute to different areas, including vaccinology and epidemiology. Full article
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15 pages, 14008 KiB  
Article
Improving Measurement Accuracy of Deep Hole Measurement Instruments through Perspective Transformation
by Xiaowei Zhao, Huifu Du and Daguo Yu
Sensors 2024, 24(10), 3158; https://doi.org/10.3390/s24103158 (registering DOI) - 16 May 2024
Abstract
Deep hole measurement is a crucial step in both deep hole machining and deep hole maintenance. Single-camera vision presents promising prospects in deep hole measurement due to its simple structure and low-cost advantages. However, the measurement error caused by the heating of the [...] Read more.
Deep hole measurement is a crucial step in both deep hole machining and deep hole maintenance. Single-camera vision presents promising prospects in deep hole measurement due to its simple structure and low-cost advantages. However, the measurement error caused by the heating of the imaging sensor makes it difficult to achieve the ideal measurement accuracy. To compensate for measurement errors induced by imaging sensor heating, this study proposes an error compensation method for laser and vision-based deep hole measurement instruments. This method predicts the pixel displacement of the entire field of view using the pixel displacement of fixed targets within the camera’s field of view and compensates for measurement errors through a perspective transformation. Theoretical analysis indicates that the perspective projection matrix changes due to the heating of the imaging sensor, which causes the thermally induced measurement error of the camera. By analyzing the displacement of the fixed target point, it is possible to monitor changes in the perspective projection matrix and thus compensate for camera measurement errors. In compensation experiments, using target displacement effectively predicts pixel drift in the pixel coordinate system. After compensation, the pixel error was suppressed from 1.99 pixels to 0.393 pixels. Repetitive measurement tests of the deep hole measurement instrument validate the practicality and reliability of compensating for thermal-induced errors using perspective transformation. Full article
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