The 2023 MDPI Annual Report has
been released!
 
19 pages, 3104 KiB  
Article
Assessing Consumer Implications of Reduced Salmon Supply and Environmental Impact in North America
by Sylvain Charlebois, Keshava Pallavi Gone, Swati Saxena, Stefanie Colombo and Bibhuti Sarker
Sustainability 2024, 16(9), 3629; https://doi.org/10.3390/su16093629 (registering DOI) - 26 Apr 2024
Abstract
This study investigates the impact of the Canadian government’s decision to reduce the supply of farm-raised salmon in British Columbia (BC) on domestic prices, the level of imports, and the environment. By drawing upon data from diverse sources, this study employs the SARIMAX [...] Read more.
This study investigates the impact of the Canadian government’s decision to reduce the supply of farm-raised salmon in British Columbia (BC) on domestic prices, the level of imports, and the environment. By drawing upon data from diverse sources, this study employs the SARIMAX model to forecast future trends in salmon prices up to 2026. The forecasted results reveal that retail salmon prices will exhibit greater unpredictability and a predicted price increase of over CAD 30 per kilogram by 2026. In addition, increased consumption of imported salmon due to BC farm closure is expected to contribute to heightened carbon emissions and result in job losses within rural and indigenous communities. In short, BC salmon farm closure carries profound consequences for both the environment and market dynamics. Full article
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22 pages, 4327 KiB  
Article
Channels of Evolution: Unveiling Evolutionary Patterns in Diatom Ca2+ Signalling
by Eleanor A. Murphy, Friedrich H. Kleiner, Katherine E. Helliwell and Glen L. Wheeler
Plants 2024, 13(9), 1207; https://doi.org/10.3390/plants13091207 (registering DOI) - 26 Apr 2024
Abstract
Diatoms are important primary producers in marine and freshwater environments, but little is known about the signalling mechanisms they use to detect changes in their environment. All eukaryotic organisms use Ca2+ signalling to perceive and respond to environmental stimuli, employing a range [...] Read more.
Diatoms are important primary producers in marine and freshwater environments, but little is known about the signalling mechanisms they use to detect changes in their environment. All eukaryotic organisms use Ca2+ signalling to perceive and respond to environmental stimuli, employing a range of Ca2+-permeable ion channels to facilitate the movement of Ca2+ across cellular membranes. We investigated the distribution of different families of Ca2+ channels in diatom genomes, with comparison to other members of the stramenopile lineage. The four-domain voltage-gated Ca2+ channels (Cav) are present in some centric diatoms but almost completely absent in pennate diatoms, whereas single-domain voltage-gated EukCatA channels were found in all diatoms. Glutamate receptors (GLRs) and pentameric ligand-gated ion channels (pLGICs) also appear to have been lost in several pennate species. Transient receptor potential (TRP) channels are present in all diatoms, but have not undergone the significant expansion seen in brown algae. All diatom species analysed lacked the mitochondrial uniporter (MCU), a highly conserved channel type found in many eukaryotes, including several stramenopile lineages. These results highlight the unique Ca2+-signalling toolkit of diatoms and indicate that evolutionary gains or losses of different Ca2+ channels may contribute to differences in cellular-signalling mechanisms between species. Full article
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28 pages, 1375 KiB  
Review
Microbial Preservation and Contamination Control in the Baking Industry
by Alane Beatriz Vermelho, Jean Vinícius Moreira, Athayde Neves Junior, Claudia Ramos da Silva, Veronica da Silva Cardoso and Ingrid Teixeira Akamine
Fermentation 2024, 10(5), 231; https://doi.org/10.3390/fermentation10050231 (registering DOI) - 26 Apr 2024
Abstract
The required processes and steps for making bread include technological and innovative concepts. The current trend is the use of less toxic compounds and green methods. Besides lactic acid bacteria and yeast, other microorganisms with unique properties, such as enzymes, new aromas and [...] Read more.
The required processes and steps for making bread include technological and innovative concepts. The current trend is the use of less toxic compounds and green methods. Besides lactic acid bacteria and yeast, other microorganisms with unique properties, such as enzymes, new aromas and flavors, exopolysaccharides, and vitamins, among other compounds with beneficial properties, could be added to bread manufacture, improving bread quality and health effects for the consumers. The preservation of microbial cultures and starters is crucial in bread-making. New encapsulation methods, cryoprotectants, spray-drying, fluidized bed drying, and vacuum drying are employed for microorganism cultures that will be used as starters or biological additives in fermentation. A development is observed in the antimicrobial methods used as bread preservatives, and studies with plant extracts and essential oils have been proposed and introduced, replacing chemical agents, such as propionate, within the clean-label bread formulations concept. Baking science is a growing research line that incorporates innovative methods, biological additives, new methods, and processes focusing on microbiological protection. Full article
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20 pages, 3089 KiB  
Article
Tannin-Tolerant Saccharomyces cerevisiae Isolated from Traditional Fermented Tea Leaf (Miang) and Application in Fruit Wine Fermentation Using Longan Juice Mixed with Seed Extract as Substrate
by Somsay Phovisay, Pratthana Kodchasee, Aliyu Dantani Abdullahi, Nang Nwet Noon Kham, Kridsada Unban, Apinun Kanpiengjai, Chalermpong Saenjum, Kalidas Shetty and Chartchai Khanongnuch
Foods 2024, 13(9), 1335; https://doi.org/10.3390/foods13091335 (registering DOI) - 26 Apr 2024
Abstract
This study focused on isolating tannin-tolerant yeasts from Miang, a fermented tea leaf product collected from northern Laos PDR, and investigating related food applications. From 43 Miang samples, six yeast isolates capable of ethanol production were obtained, with five isolates showing growth on [...] Read more.
This study focused on isolating tannin-tolerant yeasts from Miang, a fermented tea leaf product collected from northern Laos PDR, and investigating related food applications. From 43 Miang samples, six yeast isolates capable of ethanol production were obtained, with five isolates showing growth on YPD agar containing 4% (w/v) tannic acid. Molecular identification revealed three isolates as Saccharomyces cerevisiae (B5-1, B5-2, and C6-3), along with Candida tropicalis and Kazachstania humilis. Due to safety considerations, only Saccharomyces spp. were selected for further tannic acid tolerance study to advance food applications. Tannic acid at 1% (w/v) significantly influenced ethanol fermentation in all S. cerevisiae isolates. Notably, B5-2 and C6-3 showed high ethanol fermentation efficiency (2.5% w/v), while others were strongly inhibited. The application of tannin-tolerant yeasts in longan fruit wine (LFW) fermentation with longan seed extract (LSE) supplementation as a source of tannin revealed that C6-3 had the best efficacy for LFW fermentation. C6-3 showed promising efficacy, particularly with LSE supplementation, enhancing phenolic compounds, antioxidant activity, and inhibiting α-glucosidase activity, indicating potential antidiabetic properties. These findings underscore the potential of tannin-tolerant S. cerevisiae C6-3 for fermenting beverages from tannin-rich substrates like LSE, with implications for functional foods and nutraceuticals promoting health benefits. Full article
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27 pages, 4220 KiB  
Review
ADAR Family Proteins: A Structural Review
by Carolyn N. Ashley, Emmanuel Broni and Whelton A. Miller III
Curr. Issues Mol. Biol. 2024, 46(5), 3919-3945; https://doi.org/10.3390/cimb46050243 (registering DOI) - 26 Apr 2024
Abstract
This review aims to highlight the structures of ADAR proteins that have been crucial in the discernment of their functions and are relevant to future therapeutic development. ADAR proteins can correct or diversify genetic information, underscoring their pivotal contribution to protein diversity and [...] Read more.
This review aims to highlight the structures of ADAR proteins that have been crucial in the discernment of their functions and are relevant to future therapeutic development. ADAR proteins can correct or diversify genetic information, underscoring their pivotal contribution to protein diversity and the sophistication of neuronal networks. ADAR proteins have numerous functions in RNA editing independent roles and through the mechanisms of A-I RNA editing that continue to be revealed. Provided is a detailed examination of the ADAR family members—ADAR1, ADAR2, and ADAR3—each characterized by distinct isoforms that offer both structural diversity and functional variability, significantly affecting RNA editing mechanisms and exhibiting tissue-specific regulatory patterns, highlighting their shared features, such as double-stranded RNA binding domains (dsRBD) and a catalytic deaminase domain (CDD). Moreover, it explores ADARs’ extensive roles in immunity, RNA interference, and disease modulation, demonstrating their ambivalent nature in both the advancement and inhibition of diseases. Through this comprehensive analysis, the review seeks to underline the potential of targeting ADAR proteins in therapeutic strategies, urging continued investigation into their biological mechanisms and health implications. Full article
(This article belongs to the Special Issue Structure and Function of Proteins: From Bioinformatics Insights)
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25 pages, 34046 KiB  
Article
Learning to Execute Timed-Temporal-Logic Navigation Tasks under Input Constraints in Obstacle-Cluttered Environments
by Fotios C. Tolis, Panagiotis S. Trakas, Taxiarchis-Foivos Blounas, Christos K. Verginis and Charalampos P. Bechlioulis
Robotics 2024, 13(5), 65; https://doi.org/10.3390/robotics13050065 (registering DOI) - 26 Apr 2024
Abstract
This study focuses on addressing the problem of motion planning within workspaces cluttered with obstacles while considering temporal and input constraints. These specifications can encapsulate intricate high-level objectives involving both temporal and spatial constraints. The existing literature lacks the ability to fulfill time [...] Read more.
This study focuses on addressing the problem of motion planning within workspaces cluttered with obstacles while considering temporal and input constraints. These specifications can encapsulate intricate high-level objectives involving both temporal and spatial constraints. The existing literature lacks the ability to fulfill time specifications while simultaneously managing input-saturation constraints. The proposed approach introduces a hybrid three-component control algorithm designed to learn the safe execution of a high-level specification expressed as a timed temporal logic formula across predefined regions of interest in the workspace. The first component encompasses a motion controller enabling secure navigation within the minimum allowable time interval dictated by input constraints, facilitating the abstraction of the robot’s motion as a timed transition system between regions of interest. The second component utilizes formal verification and convex optimization techniques to derive an optimal high-level timed plan over the mentioned transition system, ensuring adherence to the agent’s specification. However, the necessary navigation times and associated costs among regions are initially unknown. Consequently, the algorithm’s third component iteratively adjusts the transition system and computes new plans as the agent navigates, acquiring updated information about required time intervals and associated navigation costs. The effectiveness of the proposed scheme is demonstrated through both simulation and experimental studies. Full article
(This article belongs to the Special Issue Motion Trajectory Prediction for Mobile Robots)
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12 pages, 2411 KiB  
Review
Heat/Cold Stress and Methods to Mitigate Its Detrimental Impact on Pork and Poultry Meat: A Review
by Tomasz Lesiów and Youling L. Xiong
Foods 2024, 13(9), 1333; https://doi.org/10.3390/foods13091333 (registering DOI) - 26 Apr 2024
Abstract
This paper aims to provide an updated review and current understanding of the impact of extreme temperatures—focusing on heat stress (HS)—on the quality of pork and poultry meat, particularly amidst an unprecedented global rise in environmental temperatures. Acute or chronic HS can lead [...] Read more.
This paper aims to provide an updated review and current understanding of the impact of extreme temperatures—focusing on heat stress (HS)—on the quality of pork and poultry meat, particularly amidst an unprecedented global rise in environmental temperatures. Acute or chronic HS can lead to the development of pale, soft, and exudative (PSE) meat during short transportation or of dark, firm, and dry (DFD) meat associated with long transportation and seasonal changes in pork and poultry meat. While HS is more likely to result in PSE meat, cold stress (CS) is more commonly linked to the development of DFD meat. Methods aimed at mitigating the effects of HS include showering (water sprinkling/misting) during transport, as well as control and adequate ventilation rates in the truck, which not only improve animal welfare but also reduce mortality and the incidence of PSE meat. To mitigate CS, bedding on trailers and closing the tracks’ curtains (insulation) are viable strategies. Ongoing efforts to minimize meat quality deterioration due to HS or CS must prioritize the welfare of the livestock and focus on the scaleup of laboratory testing to commercial applications. Full article
(This article belongs to the Section Meat)
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19 pages, 12273 KiB  
Article
Study on the Mechanical Properties of a Carbon-Fiber/Glass-Fiber Hybrid Foam Sandwich Structure
by Yingqiang Cai, Xiaolong Wang, Fenglin Ouyang, Qinglin Chen, Zhaoyi Zhu, Kuan Fan and Fan Ding
Materials 2024, 17(9), 2023; https://doi.org/10.3390/ma17092023 (registering DOI) - 26 Apr 2024
Abstract
Considering the different structural strength requirements of different parts of fiberglass yachts, carbon fiber/glass fiber hybrid reinforcement can be applied to the skins of sandwich panels in special areas. This paper designs and prepares 12 foam sandwich panel samples composed of pure carbon [...] Read more.
Considering the different structural strength requirements of different parts of fiberglass yachts, carbon fiber/glass fiber hybrid reinforcement can be applied to the skins of sandwich panels in special areas. This paper designs and prepares 12 foam sandwich panel samples composed of pure carbon fiber, a carbon fiber/glass fiber hybrid, pure glass fiber skin, and PVC and SAN foam sandwich, with reference to the layup structure of the outer panel of a fiberglass yacht. Through a comparative analysis of low-speed impact experiments, edge compression experiments, and short beam three-point bending experiments, we seek the optimal carbon fiber/glass fiber hybrid layup design scheme for local structures to guide production. The results show that a reasonable hybrid carbon fiber layup in fiberglass skin can effectively reduce the low-speed impact damage of the sandwich structure, reduce edge compression damage, and improve the bending and compression resistance of sandwich structure. The impact resistance, compression resistance, and shear resistance of the SAN sandwich structure are stronger than the PVC sandwich structure. The carbon fiber/glass fiber hybrid SAN foam sandwich structure can be used for the local structural reinforcement of special parts such as the bow, side, and main deck of fiberglass yachts. Full article
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13 pages, 596 KiB  
Article
Adolescent Social Anxiety, School Satisfaction, Family Emotional Support, and School Absenteeism: Findings from Young-HUNT3 and Norwegian National Education Data
by Malik D. Halidu and Yasuhiro Kotera
J. Clin. Med. 2024, 13(9), 2547; https://doi.org/10.3390/jcm13092547 (registering DOI) - 26 Apr 2024
Abstract
Background: Adolescents grappling with social anxiety may experience poor school satisfaction, resorting to school-related avoidance behaviors, exemplified by absenteeism, as a coping mechanism. Understanding the role of family support in alleviating the adverse effects of social anxiety on school satisfaction is imperative [...] Read more.
Background: Adolescents grappling with social anxiety may experience poor school satisfaction, resorting to school-related avoidance behaviors, exemplified by absenteeism, as a coping mechanism. Understanding the role of family support in alleviating the adverse effects of social anxiety on school satisfaction is imperative for fostering supportive educational settings. Although there is literature regarding how school satisfaction promotes positive adolescent outcomes, empirical knowledge on the interrelation between social anxiety, school satisfaction, and family emotional support is limited. This study investigates the association between social anxiety, family emotional support, school satisfaction, and school absenteeism within the theoretical framework of the stage-environment fit theory to offer insight into how family emotional support can moderate the influence of social anxiety on school-related outcomes. Methods: Utilizing a population-based sample of 1861 upper secondary school pupils from the Trøndelag Young Health study “Young-HUNT3 study”, we employed an index of moderated mediation to examine the role of family emotional support in moderating the association between social anxiety and school-related avoidance behavior related to school satisfaction. Results: Family emotional support had moderated mediation association for school absenteeism (β = 0.128, 95% CI 0.019, 0.278) and extracurricular activity (β = −0.003, 95% CI −0.008, −0.000). Conclusions: This urges further investigation into the specific mechanisms and individual differences influencing these relationships, aiming to deepen our understanding of adolescents’ experiences and inform comprehensive strategies for promoting their well-being within school communities. Full article
(This article belongs to the Special Issue Personalized Medicine in Children and Adolescent Psychology)
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19 pages, 7894 KiB  
Article
Lysyl Oxidase in Ectopic Cardiovascular Calcification: Role of Oxidative Stress
by Carme Ballester-Servera, Judith Alonso, Laia Cañes, Paula Vázquez-Sufuentes, Ana B. García-Redondo, Cristina Rodríguez and José Martínez-González
Antioxidants 2024, 13(5), 523; https://doi.org/10.3390/antiox13050523 (registering DOI) - 26 Apr 2024
Abstract
Lysyl oxidase (LOX)-mediated extracellular matrix crosslinking modulates calcification in atherosclerosis and aortic valve disease; however, this enzyme also induces oxidative stress. We addressed the contribution of LOX-dependent oxidative stress to cardiovascular calcification. LOX is upregulated in human-calcified atherosclerotic lesions and atheromas from atherosclerosis-challenged [...] Read more.
Lysyl oxidase (LOX)-mediated extracellular matrix crosslinking modulates calcification in atherosclerosis and aortic valve disease; however, this enzyme also induces oxidative stress. We addressed the contribution of LOX-dependent oxidative stress to cardiovascular calcification. LOX is upregulated in human-calcified atherosclerotic lesions and atheromas from atherosclerosis-challenged LOX transgenic mice (TgLOXVSMC) and colocalized with a marker of oxidative stress (8-oxo-deoxyguanosine) in vascular smooth muscle cells (VSMCs). Similarly, in calcific aortic valves, high LOX expression was detected in valvular interstitial cells (VICs) positive for 8-oxo-deoxyguanosine, while LOX and LOXL2 expression correlated with osteogenic markers (SPP1 and RUNX2) and NOX2. In human VICs, mito-TEMPO and TEMPOL attenuated the increase in superoxide anion levels and the mineralization induced by osteogenic media (OM). Likewise, in OM-exposed VICs, β-aminopropionitrile (a LOX inhibitor) ameliorated both oxidative stress and calcification. Gain- and loss-of-function approaches in VICs demonstrated that while LOX silencing negatively modulates oxidative stress and calcification induced by OM, lentiviral LOX overexpression exacerbated oxidative stress and VIC calcification, effects that were prevented by mito-TEMPO, TEMPOL, and β-aminopropionitrile. Our data indicate that LOX-induced oxidative stress participates in the procalcifying effects of LOX activity in ectopic cardiovascular calcification, and highlight the multifaceted role played by LOX isoenzymes in cardiovascular diseases. Full article
(This article belongs to the Special Issue Oxidative Stress and Atherosclerosis)
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13 pages, 3411 KiB  
Review
Gaining Insights into Key Structural Hotspots within the Allosteric Binding Pockets of Protein Kinases
by Swapnil P. Bhujbal, Joonhong Jun, Haebeen Park, Jihyun Moon, Kyungbae Min and Jung-Mi Hah
Int. J. Mol. Sci. 2024, 25(9), 4725; https://doi.org/10.3390/ijms25094725 (registering DOI) - 26 Apr 2024
Abstract
Protein kinases are essential regulators of cell function and represent one of the largest and most diverse protein families. They are particularly influential in signal transduction and coordinating complex processes like the cell cycle. Out of the 518 human protein kinases identified, 478 [...] Read more.
Protein kinases are essential regulators of cell function and represent one of the largest and most diverse protein families. They are particularly influential in signal transduction and coordinating complex processes like the cell cycle. Out of the 518 human protein kinases identified, 478 are part of a single superfamily sharing catalytic domains that are related in sequence. The dysregulation of protein kinases due to certain mutations has been associated with various diseases, including cancer. Although most of the protein kinase inhibitors identified as type I or type II primarily target the ATP-binding pockets of kinases, the structural and sequential resemblances among these pockets pose a significant challenge for selective inhibition. Therefore, targeting allosteric pockets that are beside highly conserved ATP pockets has emerged as a promising strategy to prevail current limitations, such as poor selectivity and drug resistance. In this article, we compared the binding pockets of various protein kinases for which allosteric (type III) inhibitors have already been developed. Additionally, understanding the structure and shape of existing ligands could aid in identifying key interaction sites within the allosteric pockets of kinases. This comprehensive review aims to facilitate the design of more effective and selective allosteric inhibitors. Full article
(This article belongs to the Special Issue Protein Kinase in Disease)
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11 pages, 4826 KiB  
Communication
The ARGOS Instrument for Stratospheric Aerosol Measurements
by Matthew T. DeLand, Matthew G. Kowalewski, Peter R. Colarco and Luis Ramos-Izquierdo
Remote Sens. 2024, 16(9), 1531; https://doi.org/10.3390/rs16091531 (registering DOI) - 26 Apr 2024
Abstract
Atmospheric aerosols represent an important component of the Earth’s climate system because they can contribute both positive and negative forcing to the energy budget. We are developing the Aerosol Radiometer for Global Observations of the Stratosphere (ARGOS) instrument to provide improved measurements of [...] Read more.
Atmospheric aerosols represent an important component of the Earth’s climate system because they can contribute both positive and negative forcing to the energy budget. We are developing the Aerosol Radiometer for Global Observations of the Stratosphere (ARGOS) instrument to provide improved measurements of stratospheric aerosols in a compact package. ARGOS makes limb scattering measurements from space in eight directions simultaneously, using two near-IR wavelengths for each viewing direction. The combination of forward and backward scattering views along the orbit track gives additional information to constrain the aerosol phase function and size distribution. Cross-track views provide expanded spatial coverage. ARGOS will have a demonstration flight through a hosted payload provider in the fall of 2024. The instrument has completed pre-launch environmental testing and radiometric characterization tests. The hosted payload approach offers advantages in size, weight, and power margins for instrument design compared to other approaches, with significant benefits in terms of reducing infrastructure requirements for the instrument team. Full article
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26 pages, 7233 KiB  
Article
Multiscale Modeling and Characterization of Graphene Epoxy Nanocomposite
by Collins Ekeowa and SD Jacob Muthu
Polymers 2024, 16(9), 1209; https://doi.org/10.3390/polym16091209 (registering DOI) - 26 Apr 2024
Abstract
This study aims to characterize graphene epoxy nanocomposite properties using multiscale modeling. Molecular dynamics was used to study the nanocomposite at the nanoscale and finite element analysis at the macroscale to complete the multiscale modeling. The coupling of these two scales was carried [...] Read more.
This study aims to characterize graphene epoxy nanocomposite properties using multiscale modeling. Molecular dynamics was used to study the nanocomposite at the nanoscale and finite element analysis at the macroscale to complete the multiscale modeling. The coupling of these two scales was carried out using the Irving–Kirkwood averaging method. First, the functionalization of graphene was carried and 6% grafted graphene was selected based on Young’s modulus and the tensile strength of the grafted graphene sheet. Functionalized graphene with weight fractions of 1.8, 3.7, and 5.6 wt.% were reinforced with epoxy polymer to form a graphene epoxy nanocomposite. The results showed that the graphene with 3.7 wt.% achieved the highest modulus. Subsequently, a functionalized graphene sheet with an epoxy matrix was developed to obtain the interphase properties using the MD modeling technique. The normal and shear forces at the interphase region of the graphene epoxy nanocomposite were investigated using a traction-separation test to analyze the mechanical properties including Young’s modulus and traction forces. The mean stiffness of numerically tested samples with 1.8, 3.7, and 5.6 wt.% graphene and the stiffness obtained from experimental results from the literature were compared. The experimental results are lower than the multiscale model results because the experiments cannot replicate the molecular-scale behavior. However, a similar trend could be observed for the addition of up to 3.7 wt.% graphene. This demonstrated that the graphene with 3.7 wt.% shows improved interphase properties. The macroscale properties of the graphene epoxy nanocomposite models with 1.8 and 3.7 wt.% were comparatively higher. Full article
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20 pages, 16732 KiB  
Article
Mesoscopic Analysis of Fatigue Damage Development in Asphalt Mixture Based on Modified Burgers Contact Algorithm in Discrete Element Modeling
by Mingqiao Zhou and Wei Cao
Materials 2024, 17(9), 2025; https://doi.org/10.3390/ma17092025 (registering DOI) - 26 Apr 2024
Abstract
This study is aimed at examining the mesoscopic mechanical response and crack development characteristics of asphalt mixtures using the three-dimensional discrete element approach via particle flow code (PFC). The material is considered an assembly of three phases of aggregate, mortar, and voids, for [...] Read more.
This study is aimed at examining the mesoscopic mechanical response and crack development characteristics of asphalt mixtures using the three-dimensional discrete element approach via particle flow code (PFC). The material is considered an assembly of three phases of aggregate, mortar, and voids, for which three types of contact are identified and described using a modified Burgers model allowing for bond failure and crack formation at contact. The laboratory splitting test is conducted to determine the contact parameters and to provide the basis for selecting three different load levels used in the indirect tensile fatigue test and simulation. The reliability of the simulation is verified by comparing the fatigue lives and dissipated energies against those from the test. Under cyclic loading, the internal tensile and compressive force chains vary dynamically as a response to the cyclic loading; both are initially concentrated beneath the top loading strip and then extend downward along the loading line. The compressive chains are oriented roughly vertically and develop an elliptic shape as damage grows, while the tensile chains are mostly horizontal and become denser. An analysis based on the histories of the numbers of different contact types indicates that damage mainly originates from bond failures among the aggregate particles and at the aggregate–mortar interfaces. In terms of location, cracking is initiated below the loading point (consistent with observations from the force chains) and propagates downward and laterally, leading to the macrocrack along the vertical diameter. The findings provide a mesoscopic understanding of the fatigue damage initiation and propagation in asphalt mixture. Full article
(This article belongs to the Special Issue Asphalt Mixtures and Pavements Design (2nd Edition))
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10 pages, 816 KiB  
Article
BRCA1/2 Testing Landscape in Ovarian Cancer: A Nationwide, Real-World Data Study
by Lieke Lanjouw, Joost Bart, Marian J. E. Mourits, Stefan M. Willems, Annemieke H. van der Hout, Arja ter Elst and Geertruida H. de Bock
Cancers 2024, 16(9), 1682; https://doi.org/10.3390/cancers16091682 (registering DOI) - 26 Apr 2024
Abstract
Analyzing BRCA1/2 tumor pathogenic variants (TPVs) in epithelial tubal/ovarian cancers (EOCs) has become an essential part of the diagnostic workflow in many centers to guide treatment options and genetic cascade testing. However, there is no standardization of testing procedures, including techniques, gene assays, [...] Read more.
Analyzing BRCA1/2 tumor pathogenic variants (TPVs) in epithelial tubal/ovarian cancers (EOCs) has become an essential part of the diagnostic workflow in many centers to guide treatment options and genetic cascade testing. However, there is no standardization of testing procedures, including techniques, gene assays, or sequencers used, and data on the execution of tumor tests remains scarce. Therefore, we evaluated characteristics of BRCA1/2 tumor testing in advanced-stage EOC with real-world national data. Pathology reports of patients diagnosed with EOC in 2019 in the Netherlands were obtained from the Dutch Pathology Registry (PALGA), and data regarding histological subtype and BRCA1/2 tumor tests were extracted. A total of 999 patients with advanced-stage EOC were included. Tumor tests were performed for 502 patients (50.2%) and BRCA1/2 TPVs were detected in 14.7%. Of all tests, 48.6% used hybrid capture techniques and 26.5% used PCR-based techniques. More than half of the tests (55.0%) analyzed other genes in addition to BRCA1/2. Overall, this study highlights the heterogeneity in the execution of BRCA1/2 tumor tests. Despite a lack of evidence of quality differences, we emphasize that adequate reporting and internal and external quality monitors are essential for the high-quality implementation and execution of reliable BRCA1/2 tumor testing, which is crucial for identifying all patients with BRCA1/2 TPVs. Full article
(This article belongs to the Section Cancer Pathophysiology)
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16 pages, 245 KiB  
Article
Evidence-Based Reconstruction of Memories of a Disdainful Hookup: Identifying Risk Factors and Preventing Further Victimization
by Lidia Puigvert, Ramon Flecha and Sandra Racionero-Plaza
Behav. Sci. 2024, 14(5), 367; https://doi.org/10.3390/bs14050367 (registering DOI) - 26 Apr 2024
Abstract
Autobiographical memories of close relationships have been shown to have strong influence in health and life. Yet, there is no research published about longitudinal memory reconstruction of violent sporadic relationships while reading and discussing scientific evidence on gender violence victimization. This article presents [...] Read more.
Autobiographical memories of close relationships have been shown to have strong influence in health and life. Yet, there is no research published about longitudinal memory reconstruction of violent sporadic relationships while reading and discussing scientific evidence on gender violence victimization. This article presents a novel case of the reconstruction throughout time of the memory of a disdainful hookup experienced by a young woman. The victim’s diary and an interview were the sources of data collection. The analytical categories were developed in dialogue with the participant. The results indicate that, as the subject learned scientific evidence on gender violence in sporadic relationships, she progressively recalled details of the episode that she had self-censored before, became aware of the very violent nature of the hookup, rejected the relationship, and freed her desire for satisfactory romantic relationships. Full article
23 pages, 32421 KiB  
Article
R-LRBPNet: A Lightweight SAR Image Oriented Ship Detection and Classification Method
by Gui Gao, Yuhao Chen, Zhuo Feng, Chuan Zhang, Dingfeng Duan, Hengchao Li and Xi Zhang
Remote Sens. 2024, 16(9), 1533; https://doi.org/10.3390/rs16091533 (registering DOI) - 26 Apr 2024
Abstract
Synthetic Aperture Radar (SAR) has the advantage of continuous observation throughout the day and in all weather conditions, and is used in a wide range of military and civil applications. Among these, the detection of ships at sea is an important research topic. [...] Read more.
Synthetic Aperture Radar (SAR) has the advantage of continuous observation throughout the day and in all weather conditions, and is used in a wide range of military and civil applications. Among these, the detection of ships at sea is an important research topic. Ships in SAR images are characterized by dense alignment, an arbitrary orientation and multiple scales. The existing detection algorithms are unable to solve these problems effectively. To address these issues, A YOLOV8-based oriented ship detection and classification method using SAR imaging with lightweight receptor field feature convolution, bottleneck transformers and a probabilistic intersection-over-union network (R-LRBPNet) is proposed in this paper. First, a CSP bottleneck with two bottleneck transformer (C2fBT) modules based on bottleneck transformers is proposed; this is an improved feature fusion module that integrates the global spatial features of bottleneck transformers and the rich channel features of C2f. This effectively reduces the negative impact of densely arranged scenarios. Second, we propose an angle decoupling module. This module uses probabilistic intersection-over-union (ProbIoU) and distribution focal loss (DFL) methods to compute the rotated intersection-over-union (RIoU), which effectively alleviates the problem of angle regression and the imbalance between angle regression and other regression tasks. Third, the lightweight receptive field feature convolution (LRFConv) is designed to replace the conventional convolution in the neck. This module can dynamically adjust the receptive field according to the target scale and calculate the feature pixel weights based on the input feature map. Through this module, the network can efficiently extract details and important information about ships to improve the classification performance of the ship. We conducted extensive experiments on the complex scene SAR dataset SRSDD and SSDD+. The experimental results show that R-LRBPNet has only 6.8 MB of model memory, which can achieve 78.2% detection accuracy, 64.2% recall, a 70.51 F1-Score and 71.85% mAP on the SRSDD dataset. Full article
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19 pages, 246 KiB  
Article
Enhancing COVID-19 Vaccine Uptake among Tribal Communities: A Case Study on Program Implementation Experiences from Jharkhand and Chhattisgarh States, India
by Ankita Meghani, Manjula Sharma, Tanya Singh, Sourav Ghosh Dastidar, Veena Dhawan, Natasha Kanagat, Anil Gupta, Anumegha Bhatnagar, Kapil Singh, Jessica C. Shearer and Gopal Krishna Soni
Vaccines 2024, 12(5), 463; https://doi.org/10.3390/vaccines12050463 (registering DOI) - 26 Apr 2024
Abstract
Tribal populations in India have health care challenges marked by limited access due to geographical distance, historical isolation, cultural differences, and low social stratification, and that result in weaker health indicators compared to the general population. During the pandemic, Tribal districts consistently reported [...] Read more.
Tribal populations in India have health care challenges marked by limited access due to geographical distance, historical isolation, cultural differences, and low social stratification, and that result in weaker health indicators compared to the general population. During the pandemic, Tribal districts consistently reported lower COVID-19 vaccination coverage than non-Tribal districts. We assessed the MOMENTUM Routine Immunization Transformation and Equity (the project) strategy, which aimed to increase access to and uptake of COVID-19 vaccines among Tribal populations in Chhattisgarh and Jharkhand using the reach, effectiveness, adoption, implementation, and maintenance framework. We designed a qualitative explanatory case study and conducted 90 focus group discussions and in-depth interviews with Tribal populations, community-based nongovernmental organizations that worked with district health authorities to implement the interventions, and other stakeholders such as government and community groups. The active involvement of community leaders, targeted counseling, community gatherings, and door-to-door visits appeared to increase vaccine awareness and assuage concerns about its safety and efficacy. Key adaptations such as conducting evening vaccine awareness activities, holding vaccine sessions at flexible times and sites, and modifying messaging for booster doses appeared to encourage vaccine uptake among Tribal populations. While we used project resources to mitigate financial and supply constraints where they arose, sustaining long-term uptake of project interventions appears dependent on continued funding and ongoing political support. Full article
(This article belongs to the Special Issue Inequality in Immunization 2024)
19 pages, 3969 KiB  
Article
Cytokine CCL9 Mediates Oncogenic KRAS-Induced Pancreatic Acinar-to-Ductal Metaplasia by Promoting Reactive Oxygen Species and Metalloproteinases
by Geou-Yarh Liou, Crystal J. Byrd, Peter Storz and Justin K. Messex
Int. J. Mol. Sci. 2024, 25(9), 4726; https://doi.org/10.3390/ijms25094726 (registering DOI) - 26 Apr 2024
Abstract
Pancreatic ductal adenocarcinoma (PDAC) can originate from acinar-to-ductal metaplasia (ADM). Pancreatic acini harboring oncogenic Kras mutations are transdifferentiated to a duct-like phenotype that further progresses to become pancreatic intraepithelial neoplasia (PanIN) lesions, giving rise to PDAC. Although ADM formation is frequently observed in [...] Read more.
Pancreatic ductal adenocarcinoma (PDAC) can originate from acinar-to-ductal metaplasia (ADM). Pancreatic acini harboring oncogenic Kras mutations are transdifferentiated to a duct-like phenotype that further progresses to become pancreatic intraepithelial neoplasia (PanIN) lesions, giving rise to PDAC. Although ADM formation is frequently observed in KrasG12D transgenic mouse models of PDAC, the exact mechanisms of how oncogenic KrasG12D regulates this process remain an enigma. Herein, we revealed a new downstream target of oncogenic Kras, cytokine CCL9, during ADM formation. Higher levels of CCL9 and its receptors, CCR1 and CCR3, were detected in ADM regions of the pancreas in p48cre:KrasG12D mice and human PDAC patients. Knockdown of CCL9 in KrasG12D-expressed pancreatic acini reduced KrasG12D-induced ADM in a 3D organoid culture system. Moreover, exogenously added recombinant CCL9 and overexpression of CCL9 in primary pancreatic acini induced pancreatic ADM. We also showed that, functioning as a downstream target of KrasG12D, CCL9 promoted pancreatic ADM through upregulation of the intracellular levels of reactive oxygen species (ROS) and metalloproteinases (MMPs), including MMP14, MMP3 and MMP2. Blockade of MMPs via its generic inhibitor GM6001 or knockdown of specific MMP such as MMP14 and MMP3 decreased CCL9-induced pancreatic ADM. In p48cre:KrasG12D transgenic mice, blockade of CCL9 through its specific neutralizing antibody attenuated pancreatic ADM structures and PanIN lesion formation. Furthermore, it also diminished infiltrating macrophages and expression of MMP14, MMP3 and MMP2 in the ADM areas. Altogether, our results provide novel mechanistic insight into how oncogenic Kras enhances pancreatic ADM through its new downstream target molecule, CCL9, to initiate PDAC. Full article
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16 pages, 1268 KiB  
Article
Performance of PBFT Consensus under Voting by Groups
by Vojislav B. Mišić, Jelena Mišić and Xiaolin Chang
Blockchains 2024, 2(2), 134-149; https://doi.org/10.3390/blockchains2020007 (registering DOI) - 26 Apr 2024
Abstract
Practical Byzantine Fault Tolerance (PBFT) is the protocol of choice for many applications that require distributed consensus between a number of participant nodes. While PBFT assumes a single voting committee, many applications recognize different groups of participants that need to reach a consensus [...] Read more.
Practical Byzantine Fault Tolerance (PBFT) is the protocol of choice for many applications that require distributed consensus between a number of participant nodes. While PBFT assumes a single voting committee, many applications recognize different groups of participants that need to reach a consensus separately before accepting a proposal. To this end, we propose to count the votes by separate groups or committees of participating nodes, instead of all together as in the original PBFT. We then investigate the performance impact of this approach on the mean time to accept a data block and the number of nodes involved in making the final decision. Our results indicate that the proposed solutions impose a slight performance penalty which may be countermanded by reducing the quorum numbers needed in different subsets of the original committee. Full article
(This article belongs to the Special Issue Feature Papers in Blockchains)
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13 pages, 8848 KiB  
Article
Optical Camera Communications in Healthcare: A Wearable LED Transmitter Evaluation during Indoor Physical Exercise
by Eleni Niarchou, Vicente Matus, Jose Rabadan, Victor Guerra and Rafael Perez-Jimenez
Sensors 2024, 24(9), 2766; https://doi.org/10.3390/s24092766 (registering DOI) - 26 Apr 2024
Abstract
This paper presents an experimental evaluation of a wearable light-emitting diode (LED) transmitter in an optical camera communications (OCC) system. The evaluation is conducted under conditions of controlled user movement during indoor physical exercise, encompassing both mild and intense exercise scenarios. We introduce [...] Read more.
This paper presents an experimental evaluation of a wearable light-emitting diode (LED) transmitter in an optical camera communications (OCC) system. The evaluation is conducted under conditions of controlled user movement during indoor physical exercise, encompassing both mild and intense exercise scenarios. We introduce an image processing algorithm designed to identify a template signal transmitted by the LED and detected within the image. To enhance this process, we utilize the dynamics of controlled exercise-induced motion to limit the tracking process to a smaller region within the image. We demonstrate the feasibility of detecting the transmitting source within the frames, and thus limit the tracking process to a smaller region within the image, achieving an reduction of 87.3% for mild exercise and 79.0% for intense exercise. Full article
(This article belongs to the Special Issue Recent Trends and Advances in Telecommunications and Sensing)
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30 pages, 3179 KiB  
Article
Explaining Misinformation Detection Using Large Language Models
by Vishnu S. Pendyala and Christopher E. Hall
Electronics 2024, 13(9), 1673; https://doi.org/10.3390/electronics13091673 (registering DOI) - 26 Apr 2024
Abstract
Large language models (LLMs) are a compressed repository of a vast corpus of valuable information on which they are trained. Therefore, this work hypothesizes that LLMs such as Llama, Orca, Falcon, and Mistral can be used for misinformation detection by making them cross-check [...] Read more.
Large language models (LLMs) are a compressed repository of a vast corpus of valuable information on which they are trained. Therefore, this work hypothesizes that LLMs such as Llama, Orca, Falcon, and Mistral can be used for misinformation detection by making them cross-check new information with the repository on which they are trained. Accordingly, this paper describes the findings from the investigation of the abilities of LLMs in detecting misinformation on multiple datasets. The results are interpreted using explainable AI techniques such as Local Interpretable Model-Agnostic Explanations (LIME), SHapley Additive exPlanations (SHAP), and Integrated Gradients. The LLMs themselves are also asked to explain their classification. These complementary approaches aid in better understanding the inner workings of misinformation detection using LLMs and lead to conclusions about their effectiveness at the task. The methodology is generic and nothing specific is assumed for any of the LLMs, so the conclusions apply generally. Primarily, when it comes to misinformation detection, the experiments show that the LLMs are limited by the data on which they are trained. Full article
(This article belongs to the Special Issue Advanced Natural Language Processing Technology and Applications)
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15 pages, 1869 KiB  
Article
Fabrication, Structural Characterization, and Photon Attenuation Efficiency Investigation of Polymer-Based Composites
by Sitah F. Alanazi, Norah M. Alotaibi, Mohammed Alsuhybani, Nassar Alnassar, Fahad I. Almasoud and Mansour Almurayshid
Polymers 2024, 16(9), 1212; https://doi.org/10.3390/polym16091212 (registering DOI) - 26 Apr 2024
Abstract
Experiments have assessed various polymer composites for radiation shielding in diverse applications. These composites are lighter and non-toxic when compared to lead (Pb), making them particularly effective in diagnostic imaging for shielding against low-energy photons. This study demonstrates the fabrication of four composites [...] Read more.
Experiments have assessed various polymer composites for radiation shielding in diverse applications. These composites are lighter and non-toxic when compared to lead (Pb), making them particularly effective in diagnostic imaging for shielding against low-energy photons. This study demonstrates the fabrication of four composites by combining a base material, specifically a high-density polyethylene (HDPE) polymer, with 10% and 20% silicon (Si) and silicon carbide (SiC), respectively. Additionally, 5% molybdenum (Mo) was incorporated into the composites as a heavy metal element. The composites obtained were fabricated into 20 disks with a uniform thickness of 2 mm each. Discs were exposed to radiation from a low-energy X-ray source (32.5–64.5 keV). The chemical and physical properties of composites were assessed. The shielding ability of samples was evaluated by determining the linear and mass attenuation coefficients (μ and μm), radiation protection efficiency (RPE), half-value layer (HVL), and mean free path (MFP). According to our findings, supplementing HDPE with additives improved the attenuation of beams. The μm values showed that composite X-ray shielding characteristics were enhanced with filler concentration for both Si and SiC. Polymer composites with micro-molecule fillers shelter X-rays better than polymers, especially at low energy. The HVL and MFB values of the filler are lower than those of the pure HDPE sample, indicating that less thickness is needed to shield at the appropriate energy. HC-20 blocked 92% of the incident beam at 32.5 keV. This study found that increasing the composite sample thickness or polymer filler percentage could shield against low-energy radiation. Full article
(This article belongs to the Special Issue Polymer Materials for Energy, Environment and Radiation Shielding)
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